Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| stats profiling reveals predominantly-activated stat3 in cholangiocarcinoma genesis and progression. | we investigated the aberrant expression of the stat family in humans and liver fluke (opisthorchis viverrini, ov)-induced hamster cholangiocarcinoma (cca) tissues. | 2014 | 25044480 |
| an interleukin-6 receptor polymorphism is associated with opisthorchiasis-linked cholangiocarcinoma risk in thailand. | the cholangiocarcinoma (cca) is a relatively rare cancer worldwide but it is highly prevalent in thailand where the liver fluke, opisthorchis viverrini is endemic. there are reports that interleukin 6 (il-6) may play an important role in the pathogenesis of opisthorchiasis associated cca. functionally, il-6 can act on target cells through its receptor, il-6r, and il-6r polymorphisms may affect the functional activity of il-6 leading to susceptibility to cholangiocarcinogenesis. therefore, we ass ... | 2014 | 25041016 |
| analysis of the population genetics of opisthorchis viverrini sensu lato in the nam ngum river wetland, lao pdr, by multilocus enzyme electrophoresis. | a previous population genetics study of opisthorchis viverrini from a locality in an endemic area in thailand found little genetic variation over time and second intermediate fish host species. since a similar comparative analysis is not available for lao pdr, we conducted a study of o. viverrini from different endemic foci in vientiane province, lao pdr, based on spatial, temporal and fish host species. a total of 620 adult o. viverrini originating from the nam ngum river wetland were analysed ... | 2014 | 25033811 |
| distinct mirna signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke opisthorchis viverrini. | intrahepatic cholangiocarcinoma (icc) is a significant public health problem in east asia, where it is strongly associated with chronic infection by the food-borne parasite opisthorchis viverrini (ov). we report the first comprehensive mirna expression profiling by microarray of the most common histologic grades and subtypes of icc: well differentiated, moderately differentiated, and papillary icc. | 2014 | 25017828 |
| the opisthorchis viverrini genome provides insights into life in the bile duct. | opisthorchiasis is a neglected, tropical disease caused by the carcinogenic asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is linked to malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia. no vaccine is available, and only one drug (praziquantel) is used against the parasite. little is known about o. viverrini biology and the diseases that it causes. here we characterize the draft genome (634.5 mb) and transcriptomes of o. viverrini, elucidate ... | 2014 | 25007141 |
| risk factors for opisthorchis viverrini and minute intestinal fluke infections in lao pdr, 2009-2011. | the purpose of this report is to compare the prevalence of opisthorchis viverrini/minute intestinal fluke (ov/mif) in lao people's democratic republic according to different regions and evaluate the effect of ingestion of raw freshwater fish on the infection. demographic data, behavioral data, and symptoms were obtained, and the kato-katz method was used for the diagnosis of ov/mif. in total, 3,046 individuals were enrolled. the risk factors associated with acquisition of ov/mif were raw fish co ... | 2014 | 24980495 |
| localization of the cystogenous glands of opisthorchis viverrini cercariae. | opisthorchiasis in northeastern thailand is an important etiology of cholangiocarcinoma. to form the infectious stage, free swimming cercariae penetrate cyprinid fish, shed their tails, and then secret a cystic substance to cover their larval stage to form metacercariae in the fish body. we determined the location of the cystogenous glands in opisthorchis viverrini cercariae. the cercariae and metacercariae were obtained from the naturally infected snail host, bithynia siamensis goniomphalos and ... | 2014 | 24968667 |
| first insight into cd59-like molecules of adult fasciola hepatica. | the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ... | 2014 | 24955521 |
| hepatitis b virus infection and intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is a devastating malignant tumor arising from the peripheral intrahepatic bile duct epithelium. the incidence and mortality of icc is markedly increasing over the past two decades worldwide, though the cause for this rise in incidence is unclear, thus intensifying the search for alternative etiological agents and pathogenetic mechanisms. hepatolithiasis, primary sclerosing cholangitis, parasitic infection (opisthorchis viverrini or clonorchis sinensis), fibr ... | 2014 | 24914333 |
| suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, opisthorchis viverrini. | opisthorchiasis and opisthorchis viverrini-associated bile duct cancer represent major public health threats in thailand and laos. the tegument of this food borne fluke plays pivotal roles in parasite metabolism, homeostasis and osmoregulation. excretory/secretory products also pass from the fluke to the biliary environment, products that likely underlie pathogenesis of liver fluke infection. aquaporins (aqps), belong to the major intrinsic protein superfamily of integral plasma membrane channel ... | 2014 | 24885060 |
| effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. | melatonin, a hormone secreted by the pineal gland, works directly and indirectly as a free radical scavenger. its other physiological or pharmacological activities could be dependent or independent of receptors located in different cells, organs, and tissues. in addition to its role in promoting sleep and circadian rhythms regulation, it has important immunomodulatory, antioxidant, and neuroprotective effects suggesting that this indole must be considered as a therapeutic alternative against inf ... | 2014 | 24811367 |
| experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus. | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of barbonymus gonionotus fish on infection success of o. viver ... | 2014 | 24780189 |
| prevalence of haplorchis taichui among humans and fish in luang prabang province, lao pdr. | this study confirmed the prevalence of the intestinal fluke haplorchis taichui (trematoda: heterophyidae) among people and fish in luang prabang province, lao pdr. fecal specimens were collected from 559 riparian people (229 males and 330 females), residing in 4 districts (luang prabang, xieng ngeun, pak ou, and nam bak) and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 64.9%. the positive rate for small trematode eggs (ste), which may include h ... | 2014 | 24754919 |
| patterns of trematode infections of opisthorchis viverrini (opisthorchiidae) and haplorchis taichui (heterophyidae) in human populations from two villages in savannakhet province, lao pdr. | the liver fluke, opisthorchis viverrini, and the minute intestinal fluke, haplorchis taichui, are prevalent in many asian countries. this study analysed the patterns of infections of o. viverrini and h. taichui in lahanam and thakhamlien villages (savannakhet province, lao pdr), in two cross-sectional investigations. out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for h. taichui and 65 (31.4%) for o. viverrini. both these ... | 2015 | 24739959 |
| perceived illness drives participation in mass deworming campaigns in laos. | multiple helminth infections are very common in communities of southern laos. preventive chemotherapy in combination with health education is the mainstay of control. we assessed the knowledge, perceptions and practices of rural communities related to endemic helminthiasis and their control during a mass drug administration (mda) campaign. short interviews with household heads (n=192), direct observations and discussions with attendees of mdas, and in-depth interviews with local opinion leaders ... | 2015 | 24713201 |
| rna-seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, opisthorchis viverrini. | bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, opisthorchis viverrini, the leading cause of cholangiocarcinoma (cca) in the greater mekong sub-region of thailand. despite the severe public health impact of opisthorchis-induced cca, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. the examination of differences in gene expression profiling between uninfected and o. viverrini-infected b. siamensis g ... | 2014 | 24676090 |
| intestinal parasitic infections in hiv-infected patients, lao people's democratic republic. | hiv infection is an emerging problem in laos. we conducted the first prospective study on intestinal parasites, including opportunistic protozoa, in newly diagnosed hiv infected patients, with or without diarrhea. the aims were to describe the spectrum of infections, to determine their prevalence and to assess their associations with diarrhea, cd4 cell count, place of residence and living conditions. | 2014 | 24662743 |
| prevalence of helminthic infections among inhabitants of lao pdr. | the prevalence of helminthic infections was surveyed on a total of 6,178 residents (males 2,549 and females 3,629) in 102 villages of 9 provinces in lao pdr between 2007 and 2011 under the project of korea-laos collaborative project for control of foodborne trematode infections in lao pdr. fecal specimens were collected and examined by the kato-katz thick smear and stoll's egg counting techniques. the overall liver/intestinal helminth egg positive rate was 71.9% with a single or mixed infections ... | 2014 | 24623882 |
| zoonotic trematode metacercariae in fish from phnom penh and pursat, cambodia. | a survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in phnom penh and pursat province, cambodia. all collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. in fish from phnom penh, 2 kinds of metacercariae (opisthorchis viverrini and haplorchis yokogawai) were detected. o. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fi ... | 2014 | 24623879 |
| cytokine/chemokine secretion and proteomic identification of upregulated annexin a1 from peripheral blood mononuclear cells cocultured with the liver fluke opisthorchis viverrini. | we investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (pbmcs) cocultured with adult liver flukes (opisthorchis viverrini) for 6 to 24 h. pbmc-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytokines/chemokines in the supernatant were assessed using a cytokine array. exposure to o. viverrini induced increases in secretion of proinflammatory cytokines, costimulating protein, ... | 2014 | 24614660 |
| development of a pcr assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens. | small liver and minute intestinal flukes are highly prevalent in southeast asia. definitive diagnosis of parasite infection is usually achieved parasitologically by finding the fluke eggs in feces. however, their eggs are difficult to differentiate morphologically in fecal samples, even for experienced technicians. the present study developed a pcr assay coupled with dna pyrosequencing for identification of the fish-borne trematodes (fbt), opisthorchis viverrini, clonorchis sinensis, haplorchis ... | 2014 | 24589167 |
| an ecological study of bithynia snails, the first intermediate host of opisthorchis viverrini in northeast thailand. | infection with the food-borne trematodiasis, liver fluke opisthorchis viverrini, is a major public health concern in southeast asia. while epidemiology and parasitic incidence in humans are well studied, ecological information on the o. viverrini intermediate hosts remains limited. this study aimed to investigate the factors affecting the distribution and abundance of the first intermediate host, bithynia siamensis goniomphalos snails. water quality and snails were sampled in 31 sites in muang d ... | 2015 | 24561073 |
| genomic and transcriptional alterations of cholangiocarcinoma. | cholangiocarcinoma (cca) is one of the representative cancers refractory to any therapeutic approach. the incidence of cca is highest in the northeastern part of thailand, where chronic inflammation caused by liver fluke (opisthorchis viverrini: ov) infection is a major etiologic factor. the incidence of cca is also increasing in other countries, including japan. here, we overview the genetic and transcriptional alterations of cca with and without association with ov infection. cca with ov shows ... | 2014 | 24532422 |
| high prevalence of haplorchiasis in nan and lampang provinces, thailand, proven by adult worm recovery from suspected opisthorchiasis cases. | opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in thailand. the annual surveillance reports from nan and lampang provinces, thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. this study investigated whether most cases are actually due to minute intestinal flukes (mif) rather than opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. fifty and 100 cases fr ... | 2013 | 24516289 |
| a cross-sectional study on intestinal parasitic infections in rural communities, northeast thailand. | despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in southeast asia, including thailand. in rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. this study presents a survey of the prevalence of intestinal parasitic infections among the people in rural thailand. the community-based cross-sectional study was conducted in villages in khon ... | 2013 | 24516280 |
| establishment of an allo-transplantable hamster cholangiocarcinoma cell line and its application for in vivo screening of anti-cancer drugs. | opisthorchis viverrini (o. viverrini) is a well-known causative agent of cholangiocarcinoma (cca) in humans. cca is very resistant to chemotherapy and is frequently fatal. to understand the pathogenesis of cca in humans, a rodent model was developed. however, the development of cca in rodents is time-consuming and the xenograft-transplantation model of human cca in immunodeficient mice is costly. therefore, the establishment of an in vivo screening model for o. viverrini-associated cca treatment ... | 2013 | 24516278 |
| distribution and abundance of opisthorchis viverrini metacercariae in cyprinid fish in northeastern thailand. | to increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of opisthorchis viverrini metacercariae (ov mc) was investigated in freshwater fish obtained from 20 provinces in northeastern thailand between april 2011 and february 2012. a cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. fish, were collected in each of the rainy and w ... | 2013 | 24516277 |
| curcumin prevents bile canalicular alterations in the liver of hamsters infected with opisthorchis viverrini. | opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. little is known about the pathological alterations in bile canaliculi in opisthorchiasis. this study aimed to investigate bile canalicular alterations in o. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. hamsters were infected with o. viverrini and one group of animals was fed with 1% dietary curcumin supplement. animals were examined during t ... | 2013 | 24516276 |
| molecular differentiation of opisthorchis viverrini and clonorchis sinensis eggs by multiplex real-time pcr with high resolution melting analysis. | opisthorchis viverrini and clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in asia. the standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. however, the method has low sensitivity, and eggs of o. viverrini and c. sinensis are difficult to distinguish from each other and from those of some other trematodes. here, we report a multiplex real-time pcr coupled with high resolution melting (hrm) ... | 2013 | 24516275 |
| prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand. | to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region. | 2014 | 24507641 |
| helminth infection in southern laos: high prevalence and low awareness. | helminthiasis is a public health concern in lao people's democratic republic (lao pdr, laos). this study aimed to understand helminth infection and associated risk factors in relation to existing local knowledge, attitudes and practices regarding worm infections in endemic communities. | 2013 | 24499584 |
| analysis of new biomarkers for cholangiocarcinoma. | cholangiocarcinoma is one of the most serious diseases in northeast thailand, where its incidence is reported to be the highest in the world. we tried to develop a new method to detect cholangiocarcinoma in the early stages using serum proteins. we found that after fluorescent labeling of the sugar moiety of serum proteins, a new peak was identified, which might be a promising marker for cholangiocarcinoma. | 2014 | 24446393 |
| histological confirmation of periductal fibrosis from ultrasound diagnosis in cholangiocarcinoma patients. | cholangiocarcinoma (cca) has no specific clinical signs and symptoms and non-specific bio- and tumor-markers in the early disease stage. usually patients present to tertiary care with advanced disease stage. in order to detect early cases of cca that may present as a mass, dilatation of intrahepatic duct or combination, ultrasonography is accepted as a powerful imaging tool. a smaller mass or bile duct segmental dilatation requires further imaging for characterization, including computerized tom ... | 2014 | 24420706 |
| potential targeted therapy for liver fluke associated cholangiocarcinoma. | biliary tree cancer or cholangiocarcinoma (cca) is an unusual subtype of liver cancer with exceptionally poor prognosis. lack of specific symptoms and availability of early diagnostic markers account for late diagnosis of cca. surgical treatment is a gold standard choice but few patients are candidates and local recurrence after surgery is high. benefit of systemic chemotherapy is limited; hence, better treatment options are required. the differences in etiology, anatomical positions and patholo ... | 2014 | 24408866 |
| risk biomarkers for assessment and chemoprevention of liver fluke-associated cholangiocarcinoma. | human liver fluke, opisthorchis viverrini (ov), is the major risk factor of cholangiocarcinoma (cca) in northeastern thailand. our approach focuses on genetic progression and molecular changes in the carcinogenic pathway of liver fluke-associated cca aimed at assessing patients at risk of cca and using chemoprevention as the secondary cancer prevention to reduce the incidence of cca. this review summarizes altered gene expressions, biomolecules and their modification, i.e. dna adducts, oxidized ... | 2014 | 24408859 |
| roles of liver fluke infection as risk factor for cholangiocarcinoma. | several factors are known to be associated with risk of cholangiocarcinoma (cca) and infection with the liver flukes, opisthorchis viverrini and clonorchis sinensis, has often been singled out as the leading risk factor in east and southeast asia. in this review, current knowledge of their biology, life cycle, and pathogenesis of o. viverrini, and its role as a carcinogenic parasite are presented. the trends of age-specific incidence of liver cancer in khon kaen, northeast thailand are considere ... | 2014 | 24408775 |
| retrotransposon ov-rte-1 from the carcinogenic liver fluke opisthorchis viverrini: potential target for dna-based diagnosis. | infections by the fish-borne liver flukes opisthorchis viverrini and clonorchis sinensis can lead to bile duct cancer. these neglected tropical disease pathogens occur in east asia, with o. viverrini primarily in thailand and laos and c. sinensis in cambodia, vietnam, and china. genomic information about these pathogens holds the potential to improve disease treatment and control. transcriptome analysis indicates that mobile genetic elements are active in o. viverrini, including a novel non-long ... | 2014 | 24394447 |
| dna damage in inflammation-related carcinogenesis and cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells and result in oxidative and nitrative dna damage, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in the initiation and/or promotion of inflammation-mediated carcinogen ... | 2013 | 24382987 |
| association between praziquantel and cholangiocarcinoma in patients infected with opisthorchis viverrini: a systematic review and meta-analysis. | the liver fluke, opisthorchis viverrini, and the associated incidence of subsequent cholangiocarcinoma (cca) are still a public health problem in thailand, and praziquantel (pzq) remains the antihelminthic drug of choice for treatment. evidence in hamsters shows that repeated infection and pzq treatments could increase the risk of cca. however, the existing evidence in humans is inconclusive regarding increased risk of cca with frequency of pzq intake. | 2013 | 24377641 |
| endemicity of opisthorchis viverrini liver flukes, vietnam, 2011-2012. | 2014 | 24377371 | |
| field survey focused on opisthorchis viverrini infection in five provinces of cambodia. | opisthorchiasis is endemic in thailand and lao people's democratic republic and constitutes a major public health problem throughout the mekong basin. although cambodia is located in the mekong basin, the status of o. viverrini infection in that country was not previously clarified. this research was conducted to document the extent and distribution of o. viverrini infection in cambodia. | 2014 | 24342554 |
| histopathological changes in tissues of bithynia siamensis goniomphalos incubated in crude extracts of camellia seed and mangosteen pericarp. | the present study was performed to observe histopathological changes in tissues of bithynia siamensis goniomphalos (gastropoda, bithyniidae) incubated in crude extract solutions of camellia (camellia oleifera) seed and mangosteen (garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. as the positive control, snails incubated in vari ... | 2013 | 24327779 |
| sex differences in opisthorchiosis and the development of cholangiocarcinoma in syrian hamster model. | worldwide, the highest incidence of cholangiocarcinoma (cca) is found in northeast thailand, the endemic area of opisthorchis viverrini infection. cumulated clinical data revealed that the majority of cca patients are men. however, many other types of cancers are more commonly found in women. in this study, we investigated the sex differences in the development of cca, induced by o. viverrini infection and n-nitrosodimethylamine administration, in syrian hamsters. histopathology, liver function ... | 2014 | 24318666 |
| environmental determinants of opisthorchis viverrini prevalence in northeast thailand. | opisthorchis viverrini infection is an important, human, parasitic disease along the middle and lower mekong river of southeast asia. despite vast efforts in epidemiological research, the wide geographical variation in o. viverrini prevalence remains unexplained. to investigate the potential influence of village location, prevalence data recorded during the year 2009 for 90 villages in northeast thailand were analysed by a geographical information systems approach based on a digital elevation mo ... | 2013 | 24258888 |
| dna barcode identification of freshwater snails in the family bithyniidae from thailand. | freshwater snails in the family bithyniidae are the first intermediate host for southeast asian liver fluke (opisthorchis viverrini), the causative agent of opisthorchiasis. unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. this is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. because dna barcoding, t ... | 2013 | 24223896 |
| exome sequencing identifies distinct mutational patterns in liver fluke-related and non-infection-related bile duct cancers. | the impact of different carcinogenic exposures on the specific patterns of somatic mutation in human tumors remains unclear. to address this issue, we profiled 209 cholangiocarcinomas (ccas) from asia and europe, including 108 cases caused by infection with the liver fluke opisthorchis viverrini and 101 cases caused by non-o. viverrini-related etiologies. whole-exome sequencing (n = 15) and prevalence screening (n = 194) identified recurrent somatic mutations in bap1 and arid1a, neither of which ... | 2013 | 24185513 |
| temperature dependence of opisthorchis viverrini infection in first intermediate host snail, bithynia siamensis goniomphalos. | determining of the success of a parasite's infectiveness in its snail host clearly depends on environmental conditions. temperature, one of the most influential factors impinging on metabolism of cold-blooded animals, is believed to be an important factor in parasitic infection in snails. in order to elucidate the influence of temperature, sex and size of snails on infectivity of opisthorchis viverrini to its first intermediate host, bithynia siamensis goniomphalos, 960 snails were divided into ... | 2015 | 24161535 |
| response to manuscript 'is opisthorchis viverrini an avian liver fluke?'. | 2015 | 24160764 | |
| is opisthorchis viverrini an avian liver fluke? | recently, in the journal of helminthology (may 2013), dao et al. reported that opisthorchis viverrini-like flukes were found in the bile duct of domestic ducks in vietnam. they stated that this is the first record of opisthorchis sp. in birds in vietnam. however, three opisthorchis species--o. cheelis, o. longissimus and o. parageminus--in birds in vietnam were described by le in 2000. amongst these, o. parageminus was first reported, by oshmarin in 1970, as a new opisthorchis species found in d ... | 2015 | 24160690 |
| milk fat globule epidermal growth factor 8 serves a novel biomarker of opisthorchiasis-associated cholangiocarcinoma. | milk fat globule epidermal growth factor 8 (mfg-e8) is a pleiotropic secreted glycoprotein to play roles in mediating immune tolerance and homeostasis maintenance and enhancing angiogenesis. to evaluate its value as a biomarker in opisthorchiasis-associated cholangiocarcinoma (cca), the present study investigated mfg-e8 expression kinetics during the tumorigenesis in opisthorchis viverrini infection-induced cca, and demonstrated its expression in the tumor tissues of cca patients and its serum l ... | 2014 | 24122204 |
| a combination of praziquantel and the traditional medicinal plant thunbergia laurifolia on opisthorchis viverrini infection and cholangiocarcinoma in a hamster model. | cholangiocarcinoma (cca) associated by opisthorchis viverrini remains a health problem in southeast asia including thailand. at present, there is still no efficient treatment for cca. thunbergia laurifolia is a traditionally used medicinal plant; its aqueous leave extract possesses the antioxidant activity and anti-inflammatory on hamster opisthorchiasis had been reported previously. here, we demonstrate the combined effects of the t. laurifolia extract plus antihelminthic drug, praziquantel (pz ... | 2013 | 24057695 |
| improved performance and quantitative detection of copro-antigens by a monoclonal antibody based elisa to diagnose human opisthorchiasis. | copro-antigen detection has been advocated as a promising method for diagnosis of opisthorchiasis, particularly in people that harbored light infection or have had recent drug treatment. this study aimed to improve performance of a monoclonal antibody-based enzyme-linked immunosorbent assay (mab-elisa) for detection of opisthorchis viverrini copro-antigen and assess the correlation between copro-antigen and intensity of infection. four different treatment methods of 71 samples from the lawa ende ... | 2013 | 24055716 |
| combined effects of polymorphisms of dna-repair protein genes and metabolic enzyme genes on the risk of cholangiocarcinoma. | although opisthorchis viverrini is a risk factor for cholangiocarcinoma, not all the infected individuals develop cholangiocarcinoma. we investigated whether the base excision repair enzyme gene polymorphisms with differentiated repair capacities of inflammation-related deoxyribonucleic acid damage may play a key role and such possible effects from those genes may be increased or diminished in co-existence of polymorphisms of metabolic enzymes, including glutathione-s-transferases mu 1 and gluta ... | 2013 | 24049014 |
| increase of exostosin 1 in plasma as a potential biomarker for opisthorchiasis-associated cholangiocarcinoma. | a proteomic-based approach was used to search for potential markers in the plasma of hamsters in which cholangiocarcinoma (cca) was induced by opisthorchis viverrini infection and n-nitrosodimethylamine treatment. the plasma proteins of cca-induced hamsters were resolved by 1-d page, digested by trypsin, and analyzed by lc-ms/ms. from the criteria of protein id scores >15 and an overexpression of at least three times across all time points, 37 proteins were selected. these overexpressed proteins ... | 2014 | 24018821 |
| global status of fish-borne zoonotic trematodiasis in humans. | fishborne zoonotic trematodes (fzt), infecting humans and mammals worldwide, are reviewed and options for control discussed. fifty nine species belonging to 4 families, i.e. opisthorchiidae (12 species), echinostomatidae (10 species), heterophyidae (36 species) and nanophyetidae (1 species) are listed. some trematodes, which are highly pathogenic for humans such as clonorchis sinensis, opisthorchis viverrini, o. felineus are discussed in detail, i.e. infection status in humans in endemic areas, ... | 2013 | 23990419 |
| the zoonotic, fish-borne liver flukes clonorchis sinensis, opisthorchis felineus and opisthorchis viverrini. | clonorchis sinensis, opisthorchis felineus and opisthorchis viverrini are the three most important liver flukes involved in human health, infecting more than 45 million people worldwide. both c. sinensis and o. viverrini, and possibly o. felineus, can induce human cholangiocarcinoma as well as inducing other hepatobiliary pathology. although the life cycles of all three species are similar, only that of o. felineus in europe remains predominantly zoonotic, while o. felineus in asia and c. sinens ... | 2013 | 23978669 |
| carcinogenic liver fluke opisthorchis viverrini oxysterols detected by lc-ms/ms survey of soluble fraction parasite extract. | liquid chromatography in tandem mass spectrometry (lc-ms/ms) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. here, we used lc-ms/ms to investigate in soluble extracts of the adult developmental stage of opisthorchis viverrini from experimentally infected hamsters. using comparisons with known bile acids and the metabolites of estrogens, the lc-ms data indicated the existence of novel oxysterol derivatives ... | 2013 | 23973383 |
| anti-apoptotic phenotypes of cholestan-3β,5α,6β-triol-resistant human cholangiocytes: characteristics contributing to the genesis of cholangiocarcinoma. | the oxysterols cholestan-3β,5α,6β-triol (triol) and 3-keto-cholest-4-ene (3k4) are increased in opisthorchis viverrini-associated hamster cholangiocarcinoma and induce dna damage and apoptosis via a mitochondria-dependent mechanism in mmnk-1 human cholangiocytes. based on these observations, we hypothesized that chronic exposure of cholangiocytes to these pathogenic oxysterols may allow a growth advantage to a subset of these cells through selection for resistance to apoptosis, thereby contribut ... | 2013 | 23959098 |
| infection with the carcinogenic liver fluke opisthorchis viverrini modifies intestinal and biliary microbiome. | opisthorchis viverrini is a fish-borne trematode endemic in east asia. following ingestion, the flukes locate to the biliary tre where chronic infection frequently leads to cholangiocarcinoma (cca). the mechanisms by which o. viverrini infection culminates in cca remain unknown. an unexplored aspect is its influence on the host microbiome. in the hamster, infection with this pathogen reliably leads to cca. genomic dnas of microbiota from colorectal contents and bile of hamsters and from whole o. ... | 2013 | 23925654 |
| risk factors and classifications of hilar cholangiocarcinoma. | cholangiocarcinoma is the second most common primary malignant tumor of the liver. perihilar cholangiocarcinoma or klatskin tumor represents more than 50% of all biliary tract cholangiocarcinomas. a wide range of risk factors have been identified among patients with perihilar cholangiocarcinoma including advanced age, male gender, primary sclerosing cholangitis, choledochal cysts, cholelithiasis, cholecystitis, parasitic infection (opisthorchis viverrini and clonorchis sinensis), inflammatory bo ... | 2013 | 23919107 |
| adult opisthorchis felineus major protein fractions deduced from transcripts: comparison with liver flukes opisthorchis viverrini and clonorchis sinensis. | the epidemiologically important liver flukes opisthorchis felineus, opisthorchis viverrini, and clonorchis sinensis are of interest to health professionals, epidemiologists, pharmacologists, and molecular biologists. recently the transcriptomes of the latter two species were intensively investigated. however our knowledge on molecular biology of o. felineus is scarce. we report the first results of the o. felineus transcriptome analysis. we isolated and annotated a total of 2560 expressed sequen ... | 2013 | 23891942 |
| mri and (1)h mrs evaluation for the serial bile duct changes in hamsters after infection with opisthorchis viverrini. | a 3t mr scanner was used to investigate the relationship between the alteration of bile duct lesions and the hepatic metabolic changes in hamsters infected with opisthorchis viverrini by using 3t mri and (1)h mr spectroscopy. animals were divided into control and infected groups. five normal hamsters were used as control; fifty-five hamsters were infected with o. viverrini to induce bile duct lesions and hepatic metabolic changes. t2-weighted image sequence in three orthogonal planes were conduc ... | 2013 | 23891436 |
| hyperendemicity of haplorchis taichui infection among riparian people in saravane and champasak province, lao pdr. | in this study, we found that haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in saravane and champasak province, lao pdr. fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (mekong river) districts and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 78.8% and 66.4% in saravane and champasak province, respectively. the positive rate for small ... | 2013 | 23864741 |
| does a combination of opisthorchiasis and ethyl alcohol consumption enhance early cholangiofibrosis, the risk of cholangiocarcinoma? | combination of opisthorchis viverrini infection and other factors could drive cholangiocarcinoma (cca) development in southeast asia. however, other cca factors are obscure. alcohol consumption is well known in the risk for several cancers, but there is no report in cca development. therefore, the present study was to clarify whether drinking alcohol increases the liver pathology of opisthorchis viverrini (ov) infection which may be the cca risk. experimental syrian hamsters were divided into tw ... | 2013 | 23807659 |
| infection with opisthorchis viverrini and use of praziquantel among a working-age population in northeast thailand. | infection with opisthorchis viverrini (ov) due to eating certain traditional freshwater fish dishes is the principal risk factor for cholangiocarcinoma in northeast thailand where the infection is endemic and the incidence of this form of primary liver cancer has been the highest in the world. this paper is the second report of a prospective research project to monitor the impacts of a national liver fluke control programme in a rural community of northeast thailand. a sample of 684 villagers ag ... | 2013 | 23803062 |
| a follow-up study of opisthorchis viverrini infection after the implementation of control program in a rural community, central thailand. | opisthorchis viverrini infection is still one of the public health problems in thailand. our recent cohort study conducted in a rural community in central thailand showed that the incidence rate of o. viverrini infection in 2002-2004 was 21.6/100 person-years. conventional control activities including case diagnosis and treatment, hygienic defecation promotion and health education focusing on avoiding raw fish consumption was implemented. this study aimed to re-assess the status of infection aft ... | 2013 | 23786863 |
| melatonin inhibits cholangiocarcinoma and reduces liver injury in opisthorchis viverrini-infected and n-nitrosodimethylamine-treated hamsters. | the human liver fluke opisthorchis viverrini infection and n-nitrosodimethylamine (ndma) administration induce cholangiocarcinoma (cca) and liver injury in hamsters. melatonin protects against liver injury and reduces the alteration of mitochondrial structure, mitochondrial membrane potential, and mitochondrial pro- and anti-apoptotic pathways in various cancer types. to investigate the chemopreventive effect of melatonin on cca genesis and liver injury, hamsters were treated with a combination ... | 2013 | 23772655 |
| opisthorchis viverrini-like liver fluke in birds from vietnam: morphological variability and rdna/mtdna sequence confirmation. | flukes were found in the bile ducts of domestic ducks (anas platyrhynchos), necropsied in the binh dinh province of central vietnam. following staining, morphological characteristics of the bird flukes were compatible with opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (its2) of the ribosomal dna and cytochrome c oxi ... | 2014 | 23721954 |
| timps of parasitic helminths - a large-scale analysis of high-throughput sequence datasets. | tissue inhibitors of metalloproteases (timps) are a multifunctional family of proteins that orchestrate extracellular matrix turnover, tissue remodelling and other cellular processes. in parasitic helminths, such as hookworms, timps have been proposed to play key roles in the host-parasite interplay, including invasion of and establishment in the vertebrate animal hosts. currently, knowledge of helminth timps is limited to a small number of studies on canine hookworms, whereas no information is ... | 2013 | 23721526 |
| microproteinuria during opisthorchis viverrini infection: a biomarker for advanced renal and hepatobiliary pathologies from chronic opisthorchiasis. | approximately 680 million people are at risk of infection with opisthorchis viverrini (ov) and clonorchis sinensis, with an estimated 10 million infected with ov in southeast asia alone. while opisthorchiasis is associated with hepatobiliary pathologies, such as advanced periductal fibrosis (apf) and cholangiocarcinoma (cca), animal models of ov infection show that immune-complex glomerulonephritis is an important renal pathology that develops simultaneously with hepatobiliary pathologies. a car ... | 2013 | 23717698 |
| use of black carp (mylopharyngodon piceus) in biological control of intermediate host snails of fish-borne zoonotic trematodes in nursery ponds in the red river delta, vietnam. | the risks of fish-borne zoonotic trematodes (fzt) to human health constitute an important problem in vietnam. the infection of humans with these trematodes, such as small liver trematodes (clonorchis sinensis and opisthorchis viverrini), intestinal trematodes (heterophyidae) and others is often thought to be linked to fish culture in areas where the habit of eating raw fish is common. juvenile fish produced in nurseries are often heavily infected with fzt and since fishes are sold to aquaculture ... | 2013 | 23680382 |
| annexin a1: a new immunohistological marker of cholangiocarcinoma. | to evaluate a new immunohistological marker, annexin a1 (anxa1), in cholangiocarcinoma (cca) and hepatocellular carcinoma (hcc). | 2013 | 23674846 |
| tumor-related gene changes in immunosuppressive syrian hamster cholangiocarcinoma. | the results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed syrian hamsters using prednisolone (p); normal syrian hamsters administered n-nitrosodimethylamine (nd); immunosuppressed syrian hamsters administered n-nitrosodimethylamine (ndis); normal syrian hamsters infected with opisthorchis ... | 2013 | 23645518 |
| mechanisms of oxysterol-induced disease: insights from the biliary system. | oxysterols are oxidized species of cholesterol that are derived from exogenous (e.g. dietary) and endogenous (in vivo) sources. oxysterols play critical roles in normal physiologic functions as well as in pathophysiologic processes in a variety of organ systems. this review provides an overview of oxysterol biology from the vantage point of the biliary system. several oxysterols have been identified in human bile in the context of biliary tract infection and inflammation. this finding has led to ... | 2012 | 23630545 |
| diagnosis of fasciola gigantica infection using a monoclonal antibody-based sandwich elisa for detection of circulating cathepsin b3 protease. | a reliable monoclonal antibody (moab)-based sandwich enzyme-linked immunosorbent assay (sandwich elisa) was developed for the detection of circulating cathepsin b3 protease (catb3) in the sera from mice experimentally infected with fasciola gigantica and cattle naturally infected with the same parasite. the moab 2f9 and biotinylated rabbit polyclonal anti-recombinant catb3 antibody were selected due to their high reactivities and specificities to f. gigantica catb3 antigen based on indirect elis ... | 2013 | 23567552 |
| gene expression profiling of intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is ranked as one of the top five causes of cancer-related deaths. icc in thai patients is associated with infection with the liver fluke, opisthorchis viverrini, but the molecular basis for development remains unclear. the present study employed a microarray approach to compare gene expression profiles of iccs and normal liver tissues from the same patients residing in northeast thailand, a region with a high prevalence of liver fluke infection. in icc sampl ... | 2013 | 23534794 |
| impaired antioxidant enzyme activity and increased dna repair enzyme expression in hamster liver tissues related to cholangiocarcinoma development. | a possible mechanism of liver fluke (opisthorchis viverrini; ov) -associated cholangiocarcinoma (cca) genesis may be imbalance in responses of antioxidant enzymes and/or dna repair enzymes which are the consequence of oxidative/nitrative stress, arising from inflammatory processes. this study aimed to investigate changes in the expression patterns of antioxidant enzymes, including superoxide dismutase 2 (sod2) and catalase (cat), as well as their activities in ov-associated hamster cca tissues. ... | 2012 | 23480773 |
| fishborne trematode metacercariae in luang prabang, khammouane, and saravane province, lao pdr. | fishborne trematode (fbt) metacercariae were investigated in fish from 3 provinces of lao pdr. total 242 freshwater fish of 40 species were collected in local markets of luang prabang (59 fish of 16 species), khammouane (81 fish of 19 species), and saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. four species of metacercariae (opisthorchis viverrini, haplorchis taichui, haplorchis yokogawai, and centrocestus formosanus) were detected. o. viverrini w ... | 2013 | 23467784 |
| prevalence and co-infection of intestinal parasites among thai rural residents at high-risk of developing cholangiocarcinoma: a cross-sectional study in a prospective cohort study. | intestinal parasitic infections (ipis) are still important to the health of thai rural residents. ipis are the cause of many chronic diseases with, for example, opisthorchiasis resulting in progression to cholangiocarcinoma (cca). this cross-sectional study in a prospective cohort study aimed to examine the prevalence and co- infection of intestinal parasites among northeastern thai rural residents, recruited into the khon kaen cohort study (kkcs), and who were residing in areas of high-risk for ... | 2012 | 23464426 |
| alcohol and alkalosis enhance excystation of opisthorchis viverrini metacercariae. | the northeastern region of thailand has long been known as an endemic area of the human liver fluke infection which is caused by opisthorchis viverrini. humans are infected by ingestion of uncooked cyprinoid fish in traditional dishes such as "koi-pla," "pla-som," "pla-jom," and "pla-ra." to date, the prevalence of this parasite infection remains high because of cultural behavior and local beliefs. the popular misunderstanding among people in this area is that alcohol, lemon juice, and fish sauc ... | 2013 | 23460293 |
| overexpression of microrna-21 regulating pdcd4 during tumorigenesis of liver fluke-associated cholangiocarcinoma contributes to tumor growth and metastasis. | microrna, an endogenous noncoding rna modulating gene expression, is a key molecule that by its dysregulation plays roles in inflammatory-driven carcinogenesis. this study aimed to investigate the role of oncomir mir-21 and its target, the programmed cell death 4 (pdcd4) in tumor growth and metastasis of the liver fluke opisthorchis viverrini-associated cholangiocarcinoma (cca). the expression levels of mir-21 and pdcd4 were analyzed using the taqman mirna expression assay and immunohistochemist ... | 2013 | 23417858 |
| [opisthorchiasis: tradition and innovation]. | the purpose of the review: present new data on the causative agent, pathogenesis, diagnosis and treatment of opisthorchiasis. | 2012 | 23402155 |
| proteomic analysis to identify plasma orosomucoid 2 and kinesin 18a as potential biomarkers of cholangiocarcinoma. | proteomic analysis was performed to search for the diagnostic biomarkers of the early stage of cholangiocarcinoma (cca). for this purpose, cca was experimentally induced in hamsters by the combination of n-nitrosodimethylamine (ndma) treatment and opisthorchis viverrini (ov) infection. pooled plasma of normal control, ndma-treated, ov-infected and ov+ndma (on) treated group was separated by 1-d page, and the trypsin-digested bands were analyzed with lc-ms/ms. among 82 overexpressed proteins, the ... | 2012 | 23396253 |
| human contact influences the foraging behaviour and parasite community in long-tailed macaques. | human–wildlife interactions have reached unprecedented levels, and humans are influencing the earth’s ecosystems more rapidly and extensively than ever before. this situation is cause for serious concern, especially since disease interactions between wildlife and humans have been recognized as major conservation threats. in this study, long-tailed macaques, macaca fascicularis, from 2 forest parks located in north-eastern thailand were investigated to determine the influence of habitat modificat ... | 2013 | 23363557 |
| advances with the chinese anthelminthic drug tribendimidine in clinical trials and laboratory investigations. | the anthelminthic drug tribendimidine has been approved by chinese authorities for human use in 2004, and a first comprehensive review was published in acta tropica in 2005. here, we summarise further advances made through additional clinical trials and laboratory investigations. two phase iv trials have been conducted in the people's republic of china, the first one enrolling 1292 adolescents and adults aged 15-70 years and the second one conducted with 899 children aged 4-14 years who were inf ... | 2013 | 23352956 |
| a cross-sectional study on the potential transmission of the carcinogenic liver fluke opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish. | throughout southeast asia and china, eating raw and or partially cooked cyprinid fish causes liver (hepatobiliary) disease and cancer (cholangiocarcinoma) due to fishborne zoonotic trematodes (fzt), in particular clonorchis sinensis and opisthorchis viverrini. the primary source of transmission is by native fish, but aquaculture fish are also reported to have high infective potential. here, a cross-sectional survey of fzt in fish farms was conducted in an endemic area in khon kaen province, thai ... | 2013 | 23320421 |
| opisthorchis viverrini: implications of the systematics of first intermediate hosts, bithynia snail species in thailand and lao pdr. | bithynia snails are important hosts of the human liver fluke, opisthorchis viverrini. allozyme markers were used to examine genetic variation and the systematics of bithynia snails from different geographical localities in thailand and lao pdr. our results show that bithynia siamensis goniomphalos contains two major evolutionary clades with at least nine morphologically similar but genetically distinct "cryptic" species (taxa). clade a consists of snails from the chi and the mun river wetlands, ... | 2013 | 23305887 |
| prevalence of opisthorchis viverrini infection in nakhon ratchasima province, northeast thailand. | opisthorchis viverrini infection is a serious public-health problem in southeast asia especially in lao pdr and thailand. it is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the major etiology of cholangiocarcinoma. | 2012 | 23244144 |
| molecular characterization of a tetraspanin from the human liver fluke, opisthorchis viverrini. | the human liver fluke, opisthorchis viverrini, is designated as a group 1 carcinogen, and is the major risk factor for cholangiocarcinoma in endemic countries throughout southeast asia. proteins in the excretory-secretory products and tegumental surface membranes of the fluke have been proposed to play pivotal roles in parasite survival in the host, and subsequent pathogenesis. these macromolecules are therefore valid targets for the development of vaccines and new drugs to control the infection ... | 2012 | 23236532 |
| molecular changes in opisthorchis viverrini (southeast asian liver fluke) during the transition from the juvenile to the adult stage. | the southeast asian liver fluke (opisthorchis viverrini) chronically infects and affects tens of millions of people in regions of asia, leading to chronic illness and, importantly, inducing malignant cancer (= cholangiocarcinoma). in spite of this, little is known, at the molecular level, about the parasite itself, its interplay with its hosts or the mechanisms of disease and/or carcinogenesis. | 2012 | 23209858 |
| oxidized alpha-1 antitrypsin as a predictive risk marker of opisthorchiasis-associated cholangiocarcinoma. | the oxidized alpha-1 antitrypsin (ox-a1at) is one modified form of a1at, generated via oxidation at its active site by free radicals released from inflammatory cells which subsequently are unable to inhibit protease enzymes. the presence of ox-a1at in human serum has been used as oxidative stress indicator in many diseases. as oxidative/nitrative damage is one major contributor in opisthorchiasis-driven cholangiocarcinogenesis, we determined a1at and ox-a1at expression in human cholangiocarcinom ... | 2013 | 23188705 |
| α-tocopherol and lipid profiles in plasma and the expression of α-tocopherol-related molecules in the liver of opisthorchis viverrini-infected hamsters. | opisthorchis viverrini infection induces inflammation-mediated oxidative stress and liver injury, which may alter α-tocopherol and lipid metabolism. we investigated plasma α-tocopherol and lipid profiles in hamsters infected with o. viverrini. levels of α-tocopherol, cholesterol, and low-density lipoprotein increased in the acute phase of infection. in the chronic phase, α-tocopherol decreased, while triglyceride and very low-density lipoprotein increased. notably, high-density lipoprotein decre ... | 2013 | 23178928 |
| stage-specific expression and antigenicity of glycoprotein glycans isolated from the human liver fluke, opisthorchis viverrini. | infection by opisthorchis viverrini (liver fluke) is a major public health problem in southeastern asia, resulting in hepatobiliary disease and cholangiocarcinoma. fluke surface glycoconjugates are prominently presented to the host, thereby constituting a crucial immunological interface that can determine the parasite's success in establishing infection. therefore, n- and o-linked glycoprotein glycan profiles of the infective metacercarial stage and of the mature adult were investigated by nanos ... | 2013 | 23174105 |
| community-based cross-sectional study of carcinogenic human liver fluke in elderly from surin province, thailand. | opisthorchis viverrini infection is a serious public-health problem in southeast asia. it is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. | 2012 | 23167329 |
| population genetic structuring in opisthorchis viverrini over various spatial scales in thailand and lao pdr. | khon kaen province in northeast thailand is known as a hot spot for opisthorchiasis in southeast asia. preliminary allozyme and mitochondrial dna haplotype data from within one endemic district in this province (ban phai), indicated substantial genetic variability within opisthorchis viverrini. here, we used microsatellite dna analyses to examine the genetic diversity and population structure of o. viverrini from four geographically close localities in khon kaen province. genotyping based on 12 ... | 2012 | 23166853 |
| production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of fasciola gigantica. | a monoclonal antibody (moab) against recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was produced by hybridoma technique using spleen cells from balb/c mice immunized with rfgsap-2. this moab is an igg1, κ light chain isotype. by immunoblotting and indirect elisa, the moab reacted specifically with rfgsap-2, the natural fgsap-2 at 10kda in whole body (wb) and excretory-secretory (es) fractions of f. gigantica. it did not cross react with antigens in wb fractions from other paras ... | 2013 | 23164839 |
| [role of infectious agents in the emergence of malignant tumors]. | according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ... | 2012 | 23163048 |
| species-specific primers designed from rapd products for bithynia funiculata, the first intermediate host of liver fluke, opisthorchis viverrini, in north thailand. | bithynia funiculata is the first intermediate host of the human liver fluke opisthorchis viverrini in northern thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. as a consequence, we focused on the development of species-specific primers for use as an identification tool. our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by a ... | 2013 | 23157281 |
| centrocestus formosanus (heterophyidae): human infections and the infection source in lao pdr. | in lao people's democratic republic (pdr), we detected 7 patients infected with centrocestus formosanus (1-122 adult specimens) after praziquantel treatment and purgation, together with several other trematode species including opisthorchis viverrini and haplorchis taichui. the patients were all men, 23-42 yr-of-age. three subjects were from vientiane municipality and 1 each were from khammouane, saravane, champassak, and xiengkhouang province. the patients had frequently eaten raw freshwater fi ... | 2013 | 23116489 |
| risk factors for opisthorchis viverrini infection among schoolchildren in lao pdr. | a matched-pair case-control study was conducted to identify factors associated with opisthorchis viverrini infection among primary schoolchildren to develop preventive education. a house-to-house interview of the guardians of 118 children (59 cases and 59 controls) was conducted to collect information about theirs and their children's fish eating habits of 10 locally available fish species. the guardians' knowledge and attitudes about opisthorchis viverrini infection and socio-economic status we ... | 2012 | 23077836 |