Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| synthesis and structure-activity relationships of novel benzylamine-type antifungals as butenafine-related antimycotics. | benzylamine-type antimycotics like naftifine, butenafine, or terbinafine are a well-known class of antimycotics since the 1980s. the following paper describes the synthesis and biological evaluation of a series of novel benzylamine-type antimycotics characterized by an isooctyl side chain and various substituents at the benzylamine moiety. the compounds were prepared from benzaldehyde derivatives and 2-amino-6-methylheptane by reductive amination with sodium triacetoxyborohydride and subsequent ... | 2017 | 28376264 |
| evaluation of the in vitro activity of isavuconazole and comparator voriconazole against 2635 contemporary clinical candida and aspergillus isolates. | the in vitro activity of isavuconazole was determined for 1677 candida and 958 aspergillus isolates from 2012 to 2014 with voriconazole as comparator. | 2017 | 28373148 |
| changing epidemiology of candidaemia in australia. | knowledge of contemporary epidemiology of candidaemia is essential. we aimed to identify changes since 2004 in incidence, species epidemiology and antifungal susceptibilities of candida spp. causing candidaemia in australia. | 2017 | 28364558 |
| regression analysis and categorical agreement of fluconazole disk zone diameters and minimum inhibitory concentration by broth microdilution of clinical isolates of candida. | rampant use of fluconazole in candida infections has led to predominance of less susceptible non-albicans candida over candida albicans. the aim of the study was to determine if zone diameters around fluconazole disk can be used to estimate the minimum inhibitory concentration (mic) for clinical isolates of candida species and vice versa. categorical agreement between the clinical & laboratory standards institute (clsi) recommended disk diffusion and clsi broth microdilution method was sought fo ... | 2017 | 28363817 |
| distribution and antifungal susceptibility of yeasts isolates from intensive care unit patients. | yeasts frequently colonize non-sterile sites in the body. the aim of the study was to determine distribution in clinical samples and antifungal susceptibility to five antifungals. from january 2013 through june 2015, 800 isolates were obtained from intensive care unit patients. candida albicans (58.9%), candida glabrata (20.4%), candida krusei (8.6%), and candida parapsilosis (3.6%) were the leading species. majority of the c. albicans isolates were susceptible to the fluconazole. elevated voric ... | 2017 | 28361459 |
| risk factors, characteristics, and outcomes of candidemia in an adult intensive care unit in turkey. | thirty-six patients with candidemia and 37 control patients were included consecutively to determine the characteristics of candidemia episodes. the patients with candidemia had higher mortality with higher sequential organ failure assessment scores and frequency of use of a central venous catheter, total parenteral nutrition, and broad-spectrum antibiotics; chronic renal failure with replacement therapy; and longer stay in an intensive care unit. candida albicans was the predominant species fol ... | 2017 | 28359611 |
| exogenous folates stimulate growth and budding of candida glabrata. | folate, vitamin b9, is well recognized as being essential for cell growth. the utilization of folate is common to all cells, but the source of it may be quite different. for example, mammalian cells depend on exogenous uptake of folates, while plants and microbes can synthesize them. there has been little consideration of uptake of folate in microbial cells, and studies on the effects of folates in mammalian cells, where conditions are restricted. this study shows that exogenous folates (folic a ... | 2015 | 28357288 |
| oral mucositis caused by candida glabrata biofilms: failure of the concomitant use of fluconazole and ascorbic acid. | candida glabrata is becoming one of the most prevalent pathogenic yeasts in cases of oral diseases. mucositis is an recurrent oral infection in immunocompromised patients, and the actual guidelines recommend the use of fluconazole (flu) for many cases. however, the azole resistance by c. glabrata is renowned, causing a reduced therapeutic response, especially when it occurs in biofilms. in this study, we performed an in vitro evaluation of an alternative pharmacotherapy for c. glabrata biofilm i ... | 2017 | 28357061 |
| synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid s-esters. | a series of esters of 4-acetyl, 4-trifluoroacetyl- and 4-(3-chloropropionyl)aminobenzenethiosulfoacids (twenty-four compounds) were synthesized and characterized by elemental analysis, (1)h nmr and ir spectroscopy. the antibacterial activity of the novel candidates has been screened using the agar diffusion or serial dilution methods against representative gram-positive (staphylococcus aureus, bacillus subtilis, bacillus mesentericus, mycobacterium sp., mycobacterium luteum), gram-negative (aero ... | 2017 | 28344478 |
| identification of genetic markers of resistance to echinocandins, azoles and 5-fluorocytosine in candida glabrata by next generation sequencing: a feasibility study. | multi-antifungal drug resistance in candida glabrata is increasing. we examined the feasibility of next generation sequencing (ngs) to investigate the presence of antifungal drug resistance markers in c. glabrata. | 2017 | 28344162 |
| identification of a polysaccharide produced by the pyruvate overproducer candida glabrata cctcc m202019. | candida glabrata has great potential for the accumulation of pyruvate as a preferred strain in pyruvate production by fermentation. however, its substrate conversion rate is relatively low. in this study, a novel polysaccharide containing α-1,4-glucosidic bonds was observed accidentally in screening a high-titer pyruvate strain by atmospheric and room temperature plasma mutagenesis of c. glabrata. chemical analysis of the partially purified polysaccharide s4-c10 showed the main components were 1 ... | 2017 | 28343242 |
| epidemiology, species distribution, clinical characteristics and mortality of candidaemia in a tertiary care university hospital in turkey, 2007-2014. | candidaemia still continues to be a serious medical concern and the epidemiology of candidaemia varies according to geographical areas. we aim to determine the incidence, local epidemiology, candida species distribution and crude mortality rates of candidaemia. we retrospectively evaluated candidaemia episodes in between january 2007 and august 2014. we compared demographic, clinical, microbiological findings and mortality rates of episodes caused by candida albicans and non-albicans candida spe ... | 2017 | 28338249 |
| cutaneous septic emboli from candida glabrata in a haematological patient. | 2017 | 28335079 | |
| predisposing factors and outcome of uncommon yeast species-related fungaemia based on an exhaustive surveillance programme (2002-14). | using registry data to compare fungaemia caused by uncommon yeast species (uys; i.e. other than candida albicans , candida glabrata , candida parapsilosis , candida tropicalis and candida krusei ) and c. albicans -related fungaemia can reveal specific predisposing factors of uys with potential impact on treatment strategies. | 2017 | 28333259 |
| water quality and antifungal susceptibility of opportunistic yeast pathogens from rivers. | yeasts from water sources have been associated with diseases ranging from superficial mucosal infections to life threatening diseases. the aim of this study was to determine the water quality as well as diversity and antifungal susceptibility of yeasts from two rivers. yeast levels and physico-chemical parameter data were analyzed by principal component analysis to determine correlations between physico-chemical data and yeast levels. yeast morphotypes were identified by biochemical tests and 26 ... | 2017 | 28333048 |
| metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india. | this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ... | 2016 | 28330225 |
| echinocandins compared to fluconazole for candidemia of a urinary tract source: a propensity score analysis. | whether echinocandins could be used to treat candidemia of a urinary tract source (cuts) is unknown. we aimed to provide current epidemiological information of cuts and to compare echinocandin to fluconazole treatment on cuts outcomes. | 2017 | 28329281 |
| the berkeleylactones, antibiotic macrolides from fungal coculture. | a carefully timed coculture fermentation of penicillium fuscum and p. camembertii/clavigerum yielded eight new 16-membered-ring macrolides, berkeleylactones a-h (1, 4, 6-9, 12, 13), as well as the known antibiotic macrolide a26771b (5), patulin, and citrinin. there was no evidence of the production of the berkeleylactones or a26771b (5) by either fungus when grown as axenic cultures. the structures were deduced from analyses of spectral data, and the absolute configurations of compounds 1 and 9 ... | 2017 | 28326781 |
| antimicrobial percutaneous nephrostomy catheters for the prevention of renal infections: in vitro studies. | purpose - percutaneous nephrostomy (pcn) catheters are the primary method for draining ureters obstructed by malignancy and preventing a decline of the renal function. however, pcn-related infections such as pyelonephritis and urosepsis remain a significant concern. currently no antimicrobial pcns are available for preventing infectious complications. vascular catheters impregnated with minocycline/rifampin (m/r) and m/r with chlorhexidine coating (m/r+chd) have previously demonstrated antimicro ... | 2017 | 28320713 |
| epidemiological and mycological characteristics of candidemia in iran: a systematic review and meta-analysis. | to date, there has been no comprehensive review of the epidemiology, risk factors, species distribution, and outcomes of candidemia in iran. this study aimed to perform a systematic review and meta-analysis of all reported candidemia cases in iran until december 2015. the review process occurred in three steps, namely a literature search, data extraction and statistical analyses. after a comprehensive literature search, we identified 55 cases. the mean age of patients was 46.80±24.30 years (rang ... | 2017 | 28318900 |
| vulvovaginal candidiasis: etiology, symptomatology and risk factors. | to determine epidemiological, clinical and mycological characteristics of vulvovaginal candidiasis (vvc) in tunisian population and to evaluate predisposing factors. | 2017 | 28314677 |
| antifungal drug susceptibility of candida species isolated from hiv-positive patients recruited at a public hospital in são luís, maranhão, brazil. | oropharyngeal candidiasis is the most common fungal infection in hospitalized patients with acquired immune deficiency syndrome (aids). its progression results in invasive infections, which are a significant cause of morbidity and mortality. this study aimed to quickly and accurately identify candida spp. from oral mucosa of aids patients recruited at presidente vargas hospital, in são luís city, brazil and to evaluate the sensitivity profile of these fungi to antifungals by using an automated s ... | 2017 | 28303122 |
| mechanistic insights underlying tolerance to acetic acid stress in vaginal candida glabrata clinical isolates. | during colonization of the vaginal tract candida glabrata cells are challenged with the presence of acetic acid at a low ph, specially when dysbiosis occurs. to avoid exclusion from this niche c. glabrata cells are expected to evolve efficient adaptive responses to cope with this stress; however, these responses remain largely uncharacterized, especially in vaginal strains. in this work a cohort of 18 vaginal strains and 2 laboratory strains (cbs138 and kue100) were phenotyped for their toleranc ... | 2017 | 28293217 |
| binding of candida albicans to human ceacam1 and ceacam6 modulates the inflammatory response of intestinal epithelial cells. | candida albicans colonizes human mucosa, including the gastrointestinal tract, as a commensal. in immunocompromised patients, c. albicans can breach the intestinal epithelial barrier and cause fatal invasive infections. carcinoembryonic antigen-related cell adhesion molecule 1 (ceacam1; cd66a), ceacam5 (cea), and ceacam6 (cd66c) are immunomodulatory receptors expressed on human mucosa and are recruited by bacterial and viral pathogens. here we show for the first time that a fungal pathogen (i.e. ... | 2017 | 28292985 |
| candida krusei form mycelia along agar surfaces towards each other and other candida species. | candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. we noticed another possible interaction on agar surfaces, where c. krusei yeast cells formed mycelia along agar surfaces toward each other. we report here the results of experiments to study this interaction. | 2017 | 28284180 |
| draft genome sequences of candida glabrata isolates 1a, 1b, 2a, 2b, 3a, and 3b. | here, we report the draft genome sequences of six candida glabrata isolates. the isolates were taken from blood samples from patients after recurrent c. glabrata infection. two isolates were taken from each of three patients a minimum 3 months apart. | 2017 | 28280017 |
| disseminated phaeohyphomycosis in a dog. | phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to bipolaris spicifera infection. regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. candida glabrata and fusarium oxysporum were also cultured from multiple sites post-mortem. the dog was treated with fluconazole, itraconazole, t ... | 2017 | 28275534 |
| a risk score for fluconazole failure among patients with candidemia. | background: this study aimed to develop a prediction model to identify patients with candidemia at high risk of failing fluconazole.methods: adult patients in the us with candidemia who received fluconazole during hospitalization were selected from the cerner health facts® database (04/2004-03/2013). fluconazole failure was defined as switching/adding another antifungal, positive candida culture ≥10 days after fluconazole initiation, or death during hospitalization. patients were randomized into ... | 2017 | 28264843 |
| utilization of size polymorphism in its1 and its2 regions for identification of pathogenic yeast species. | despite the existence of a variety of available yeast-identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. the internal transcribed spacer (its) regions of fungal rrna genes exhibit variable sizes depending on the yeast species. in the present study, fragment size polymorphism (fsp) analysis of the its1 and its2 regions for identification of the clinically most important yeast species was assessed. | 2017 | 28260588 |
| structural analyses of candida albicans sterol 14α-demethylase complexed with azole drugs address the molecular basis of azole-mediated inhibition of fungal sterol biosynthesis. | with some advances in modern medicine (such as cancer chemotherapy, broad exposure to antibiotics, and immunosuppression) the incidence of opportunistic fungal pathogens such as candida albicans has increased. cases of drug resistance among these pathogens have become more frequent, requiring the development of new drugs and a better understanding of the targeted enzymes. sterol 14α-demethylase (cyp51) is a cytochrome p450 enzyme required for biosynthesis of sterols in eukaryotic cells and the m ... | 2017 | 28258218 |
| studying the prevalence, species distribution, and detection of in vitro production of phospholipase from candida isolated from cases of invasive candidiasis. | candida spp. have emerged as successful pathogens both in invasive and mucosal infections. c. albicans is the sixth cause of most common nosocomial infections according to studies by the centers for disease control and prevention. a shift toward non-albicans species has been reported. there is a dearth of knowledge regarding the virulence factors of candida, especially from this part of india. the aim was to study the prevalence of candida, speciate, and determine antifungal sensitivity along wi ... | 2017 | 28250619 |
| in vitro and in vivo antifungal profile of a novel and long acting inhaled azole, pc945, on aspergillus fumigatus infection. | the profile of pc945, a novel triazole antifungal, designed for administration via inhalation, has been assessed in a range of in vitro and in vivo studies. pc945 was characterized as a potent, tight-binding inhibitor of aspergillus fumigatus sterol 14α-demethylase (cyp51a and cyp51b) activity (ic50, 0.23 μm and 0.22 μm, respectively), with characteristic type ii azole binding spectra. against 96 clinically isolated a. fumigatus strains, the mic values of pc945 ranged from 0.032∼>8 μg/ml, whilst ... | 2017 | 28223388 |
| prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant gram-negative bacilli and yeast in intestinal flora of cancer patients in north lebanon. | the emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. the aim of the study is to determine the prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts in cancer patients under chemotherapy. 41 stool samples collected from cancer patients in nini hospital in tripoli, north lebanon have been analyzed. after isolating yeasts and carbapenem-resistant bacteria, a biochemical identification and antimicrobial susceptibility ... | 2017 | 28216021 |
| in vitro combination therapy with isavuconazole against candida spp. | combination therapy may be an alternative therapeutic approach for difficult-to-treat candida infections with the aim of increasing efficacy of antifungal therapy. whether isavuconazole, an extended-spectrum triazole, possesses synergistic activity in combination therapy with echinocandins or polyenes for the treatment of invasive candidiasis has not been studied. we used bliss independence drug interaction analysis and time-kill assays to examine the in vitro interactions of isavuconazole with ... | 2017 | 28204571 |
| [cancer and mycoses and literature review]. | various infectious agents are classical risk factors for cancer including bacteria, viruses and parasites. there is less evidence concerning the implication of fungal infection in carcinogenesis. the role of chronic candida infection in the development of squamous cell carcinoma has been suspected for years. candida sp are more prevalent in potentially malignant disorder and cancer of the oral mucosa. other epidemiological evidence of a link between candida infection and cancer is what is observ ... | 2017 | 28188605 |
| antifungal activity of two root canal sealers against different strains of candida. | microorganisms and microbial products are the main etiologic factors in pulp and periapical diseases. the present study aimed to compare the antifungal activity of two different sealers, ah-26 and mta fillapex against three strains of candida, 24, 48, 72 h and 7 days after mixing. | 2017 | 28179934 |
| the novel oral glucan synthase inhibitor scy-078 shows in vitro activity against sessile and planktonic candida spp. | we studied the antifungal activity of scy-078 (an orally bioavailable 1,3-β -d- glucan synthesis inhibitor), micafungin and fluconazole against the planktonic and sessile forms of 178 candida and non- candida isolates causing fungaemia in patients recently admitted to a large european hospital. | 2017 | 28175309 |
| candida krusei and candida glabrata reduce the filamentation of candida albicans by downregulating expression of hwp1 gene. | pathogenicity of candida albicans is associated with its capacity switch from yeast-like to hyphal growth. the hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. therefore, many investigations have focused on mechanisms that control the morphological transitions of c. albicans. recently, certain studies have showed that non-albicans candida species can reduce the capacity of c. albicans to form biofilms and to develop candidiasis in animal models. then, t ... | 2017 | 28164244 |
| epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity. | this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ... | 2017 | 28163074 |
| fungicidal efficacy of various honeys against fluconazole-resistant candida species isolated from hiv(+) patients with candidiasis. | honey is well known to possess a broad spectrum of activity against medically important organisms. the purpose of this study was to assess the antifungal activity of different honeys against 40 fluconazole (flu) resistant candida species, including candida albicans (c. albicans), candida glabrata, candida krusei and candida tropicalis. | 2017 | 28159362 |
| in vitro activity of the novel echinocandin cd101 at ph 7 and 4 against candida spp. isolates from patients with vulvovaginal candidiasis. | the novel echinocandin cd101 has stability properties amenable to topical formulation for use in the treatment of acute vulvovaginal candidiasis (vvc) and recurrent vvc (rvvc). cd101 has demonstrated potent antifungal activity at ph 7, but assessment of its activity at the physiological ph of the vaginal environment is needed. | 2017 | 28158577 |
| antifungal activity of lactic acid bacteria strains isolated from natural honey against pathogenic candida species. | the role of lactic acid bacteria (lab) in honey as antifungal activity has received little attention and their mechanism of inhibitory of fungi is not fully understood. in this study, lab were isolated from honey samples from malaysia, libya, saudi arabia, and yemen. twenty-five isolates were confirmed lab by catalase test and gram staining, and were screened for antifungal activity. four lab showed inhibitory activity against candida spp. using the dual agar overlay method. and they were identi ... | 2016 | 28154488 |
| lipid signaling via pkh1/2 regulates fungal co2 sensing through the kinase sch9. | adaptation to alternating co2 concentrations is crucial for all organisms. carbonic anhydrases-metalloenzymes that have been found in all domains of life-enable fixation of scarce co2 by accelerating its conversion to bicarbonate and ensure maintenance of cellular metabolism. in fungi and other eukaryotes, the carbonic anhydrase nce103 has been shown to be essential for growth in air (~0.04% co2). expression of nce103 is regulated in response to co2 availability. in saccharomyces cerevisiae, nce ... | 2017 | 28143980 |
| differential effects of the combination of tyrosol with chlorhexidine gluconate on oral biofilms. | this study assessed the effect of tyrosol and chlorhexidine gluconate in combination against candida albicans, candida glabrata, and streptococcus mutans in the planktonic state or forming biofilms in vitro. | 2017 | 28142218 |
| epidemiological investigation of candida species causing bloodstream infection in paediatric small bowel transplant recipients. | small bowel transplantation (sbt) can be a life-saving medical procedure. however, these recipients experience high risk of bloodstream infections caused by candida. this research aims to characterise the sbt recipient gut microbiota over time following transplantation and investigate the epidemiology of candidaemia in seven paediatric patients. candida species from the recipients' ileum and bloodstream were identified by internal transcribed spacer sequence and distinguished to strain by multil ... | 2017 | 28139856 |
| recombinant 3-hydroxy 3-methyl glutaryl-coa reductase from candida glabrata (rec-cghmgr) obtained by heterologous expression, as a novel therapeutic target model for testing synthetic drugs. | the enzyme 3-hydroxy-3-methyl-glutaryl coa reductase (hmgr) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. this enzyme has three domains: transmembrane, binding, and soluble. in this study, we expressed and purified the soluble fraction of the hmgr enzyme from candida glabrata (cghmgr) in an escherichia coli heterologous system and used it as a model for studying its inhibitory activity. t ... | 2017 | 28138930 |
| cyclodextrin-mediated self-associating chitosan micro-platelets act as a drug booster against candida glabrata mucosal infection in immunocompetent mice. | this study reports design and evaluation of chitosan-based microparticle activity against candida glabrata in vitro and in vivo in immunocompetent mice model artificially maintained in oestrus state. because their flattened shape, chitosan microparticles are called here micro-platelets. they were obtained by self-association of oleoyl chitosan and α-cyclodextrin in water. a mixture of amphotericin b-deoxycholate (fungizone(®), amb-doc) and chitosan micro-platelets gelified with pluronic(®) f127 ... | 2017 | 28130196 |
| azole antifungal resistance in candida albicans and emerging non-albicans candida species. | within the limited antifungal armamentarium, the azole antifungals are the most frequent class used to treat candida infections. azole antifungals such as fluconazole are often preferred treatment for many candida infections as they are inexpensive, exhibit limited toxicity, and are available for oral administration. there is, however, extensive documentation of intrinsic and developed resistance to azole antifungals among several candida species. as the frequency of azole resistant candida isol ... | 2016 | 28127295 |
| [evaluation of pna-fish method for direct identification of candida species in blood culture samples and its potential impact on guidance of antifungal therapy]. | early antifungal therapy has a major influence on survival in candidemia. rapid identification of the species has importance for the treatment, prediction of the species-specific primary resistance and variable antifungal susceptibility. recently, molecular-based methods attempt to reduce the time between the positive signal of a blood culture and identification of the fungus. pna-fish (peptide nucleic acid fluorescence in situ hybridization) assay distinguishes a number of frequently isolated c ... | 2016 | 28124963 |
| in vitro antifungal susceptibility of oral candida isolates from patients suffering from caries and chronic periodontitis. | caries and chronic periodontitis are common oral diseases where a higher candida colonization is reported. antifungal agents could be adjuvant drugs for the therapy of both clinical conditions. the aim of the current study has been to evaluate the in vitro activities of conventional and new antifungal drugs against oral candida isolates from patients suffering from caries and/or chronic periodontitis. in vitro activities of amphotericin b, fluconazole, itraconazole, miconazole, nystatin, posacon ... | 2017 | 28124220 |
| granule-dependent natural killer cell cytotoxicity to fungal pathogens. | natural killer (nk) cells kill or inhibit the growth of a number of fungi including cryptococcus, candida, aspergillus, rhizopus, and paracoccidioides. although many fungi are not dangerous, invasive fungal pathogens, such as cryptococcus neoformans, cause life-threatening disease in individuals with impaired cell-mediated immunity. while there are similarities to cell-mediated killing of tumor cells, there are also important differences. similar to tumor killing, nk cells directly kill fungi in ... | 2016 | 28123389 |
| a high-throughput screening procedure for enhancing pyruvate production in candida glabrata by random mutagenesis. | candida glabrata is one of the most promising pyruvate producers. a series of experiments was conducted to enhance pyruvate production by c. glabrata via metabolic and genetic engineering. here, a novel screening strategy, which combined atmospheric and room temperature plasma-based random mutagenesis and high-throughput screening (hts), was used to screen for high pyruvate-producing mutants that could use cheap industrial raw materials as nitrogen sources. a high-titer pyruvate producer (h6) wa ... | 2017 | 28120126 |
| analysis of polyphenolic compounds in extracts from leaves of some malus domestica cultivars: antiradical and antimicrobial analysis of these extracts. | in this study, methanol, ethyl acetate, water extracts, and precipitate were obtained from leaves of malus domestica cultivars: golden delicious, jonagold, elstar, ligol, and mutsu. antiradical activity of these extracts was measured using the abts(+∙) radical, and antimicrobial activity was measured with the disk-diffusion method. phenolic compounds were measured with the colorimetric method and identified with high performance liquid chromatography (hplc). the highest antiradical activity was ... | 2016 | 28097143 |
| antifungal activity of substituted aurones. | novel antifungals are in high demand as there is a growing resistance to antifungals currently in use. in particular, opportunistic fungal infections caused by candida spp. are on the rise with infections by this genus accounting for the most severe fungal infections following chemotherapy, implantation procedures, and in patients with hiv/aids. a series of simple aurone analogs were synthesized and screened for antifungal activity versus candida spp. several compounds displayed activity at 100μ ... | 2017 | 28094180 |
| repeated applications of photodynamic therapy on candida glabrata biofilms formed in acrylic resin polymerized. | previous studies have been suggested that photodynamic therapy (pdt) can be used as an adjuvant treatment for denture stomatitis. in this study, we evaluated the effects of multiple sessions of pdt on candida glabrata biofilms in specimens of polymerized acrylic resin formed after 5 days. subsequently, four applications of pdt were performed on biofilms in 24-h intervals (days 6-9). also, we evaluated two types of pdt, including application of laser and methylene blue or light-emitting diode (le ... | 2017 | 28091850 |
| pharmacokinetic study of anidulafungin in icu patients with intra-abdominal candidiasis. | only limited pharmacokinetic data are available for anidulafungin in icu patients, especially in patients treated for severe intra-abdominal infection (iai). | 2017 | 28088767 |
| micafungin: a review in the prophylaxis and treatment of invasive candida infections in paediatric patients. | intravenous micafungin (mycamine(®); funguard(®)), an echinocandin, is approved in the eu for the treatment of invasive candidiasis in children (including neonates) and adolescents (<16 years of age) and as prophylaxis against candida infections in patients undergoing haematopoietic stem cell transplantation (hsct) or who are expected to have neutropenia for ≥10 days. this narrative review focuses on the use of micafungin in paediatric indications approved in the eu, which may vary from those ap ... | 2017 | 28083856 |
| scy-078 is fungicidal against candida species in time-kill studies. | scy-078 is an orally bioavailable ß-1,3-glucan synthesis inhibitor (gsi) and the first-in-class of structurally novel triterpene antifungals in clinical development for treating candidemia and invasive candidiasis. in vitro susceptibilities by broth microdilution, antifungal carryover, and time-kill dynamics were determined for three reference (atcc) strains (candida albicans 90028, candida parapsilosis 90018, and candida tropicalis 750), a quality-control (qc) strain (candida krusei 6258), and ... | 2017 | 28069658 |
| utilization of size polymorphism in its1 and its2 regions for identification of pathogenic yeast species. | despite the existence of a variety of available yeast identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. the internal transcribed spacer (its) regions of fungal rrna genes exhibit variable sizes depending on the yeast species. in the present study, fragment size polymorphism (fsp) analysis of the its1 and its2 regions for identification of the clinically most important yeast species was assessed. the its1 and its2 regions of 1 ... | 2017 | 28068213 |
| membrane proteomics analysis of the candida glabrata response to 5-flucytosine: unveiling the role and regulation of the drug efflux transporters cgflr1 and cgflr2. | resistance to 5-flucytosine (5-fc), used as an antifungal drug in combination therapy, compromises its therapeutic action. in this work, the response of the human pathogen candida glabrata to 5-fc was evaluated at the membrane proteome level, using an itraq-based approach. a total of 32 proteins were found to display significant expression changes in the membrane fraction of cells upon exposure to 5-fc, 50% of which under the control of cgpdr1, the major regulator of azole drug resistance. these ... | 2016 | 28066366 |
| fluconazole and echinocandin resistance of candida glabrata correlates better with antifungal drug exposure rather than with msh2 mutator genotype in a french cohort of patients harboring low rates of resistance. | candida glabrata is a major pathogenic yeast in humans that is known to rapidly acquire resistance to triazole and echinocandin antifungal drugs. a mutator genotype (msh2 polymorphism) inducing a mismatch repair defect has been recently proposed to be responsible for resistance acquisition in c. glabrata clinical isolates. our objectives were to evaluate the prevalence of antifungal resistance in a large cohort of patients in saint-louis hospital, paris, france, some of whom were pre-exposed to ... | 2016 | 28066361 |
| widespread inter- and intra-domain horizontal gene transfer of d-amino acid metabolism enzymes in eukaryotes. | analysis of the growing number of available fully-sequenced genomes has shown that horizontal gene transfer (hgt) in eukaryotes is more common than previously thought. it has been proposed that genes with certain functions may be more prone to hgt than others, but we still have a very poor understanding of the selective forces driving eukaryotic hgt. recent work uncovered that d-amino acid racemases have been commonly transferred from bacteria to fungi, but their role in the receiving organisms ... | 2016 | 28066338 |
| pan-azole-resistant candida tropicalis carrying homozygous erg11 mutations at position k143r: a new emerging superbug? | candidaemia is a public health problem mainly in hospitalized individuals worldwide. in brazil, candida albicans is the most prevalent species that causes candidaemia, followed by candida tropicalis and candida parapsilosis . few data on the abundance of antifungal resistance are available for latin america. | 2017 | 28065893 |
| activity of a long-acting echinocandin (cd101) and seven comparator antifungal agents tested against a global collection of contemporary invasive fungal isolates in the sentry 2014 antifungal surveillance program. | the activity of cd101 and comparator antifungal agents against 606 invasive fungal isolates collected worldwide during 2014 was evaluated using the clinical and laboratory standards institute (clsi) method. all candida albicans (n = 251), candida tropicalis (n = 51), candida krusei (n = 16), and candida dubliniensis (n = 11) isolates were inhibited by ≤0.12 μg/ml of cd101 and were susceptible or showed wild-type susceptibility to the other echinocandins tested. five c. glabrata isolates (n = 100 ... | 2017 | 28052853 |
| caspofungin mic distribution amongst commonly isolated candida species in a tertiary care centre - an indian experience. | emergence of candida species resistant to amphotericin b and triazole has led to use of echinocandins, mostly caspofungin in the management of invasive candidiasis. there are some published reports of caspofungin resistance in candida species yet no studies on caspofungin susceptibility pattern of candida species exist in indian setup. | 2016 | 28050365 |
| activity of the aqueous extract of schinus terebinthifolius raddi on strains of the candida genus. | objectives to evaluate the antifungal susceptibility profile of the aqueous extract of the bark of schinus terebinthifolius raddi against the strains of the genus candida. methods by using the disk diffusion method, 50 samples of the genus candida (candida albicans; candida krusei; candida glabrata; and candida tropicalis), isolated from patients receiving treatment at hospital santa casa de misericórdia de são paulo, and 1 american type culture collection (atcc) sample of each species were test ... | 2016 | 28038478 |
| effects of aspirin as an anti-inflammatory drug on azole-resistant candida glabrata in vitro. | 2016 | 28028508 | |
| fungicidal effect of thymoquinone involves generation of oxidative stress in candida glabrata. | the antifungal effect of thymoquinone, a component of black seed essential oil, has been studied on different types of fungi. its mechanism of action as an antifungal has not been described yet. this study demonstrates the fungicidal effect of thymoquinone on different candida species with particular emphasis on c. glabrata planktonic cells and biofilms. since cell death was induced via the generation of oxidative stress as evidenced by the abrogation of thymoquinone toxicity in cells incubated ... | 2017 | 28024529 |
| genetic drivers of multidrug resistance in candida glabrata. | both the incidence of invasive fungal infections and rates of multidrug resistance associated with fungal pathogen candida glabrata have increased in recent years. in this perspective, we will discuss the mechanisms underlying the capacity of c. glabrata to rapidly develop resistance to multiple drug classes, including triazoles and echinocandins. we will focus on the extensive genetic diversity among clinical isolates of c. glabrata, which likely enables this yeast to survive multiple stressors ... | 2016 | 28018323 |
| new formyl phloroglucinol meroterpenoids from the leaves of eucalyptus robusta. | seven new formyl phloroglucinol meroterpenoids (fpms), namely eucalrobusones j-p (1-7), as well as three known ones (8-10) were isolated from the leaves of eucalyptus robusta. their structures were elucidated by spectroscopic data analysis, and their absolute configurations were determined by applications of the snatzke's helicity rule and the electron circular dichroism (ecd) calculation. these fpms are diverse in coupling patterns between phloroglucinol and sesquiterpenoid units, forming novel ... | 2016 | 28004790 |
| antimicrobial and antioxidant activities of a new metabolite from quercus incana. | phytochemical investigations of quercus incana led to the isolation of a new catechin derivative quercuschin (1), along with six known compounds: quercetin (2), methyl gallate (3), gallic acid (4), betulinic acid (5), (z)-9-octadecenoic acid methyl ester (6) and β-sitosterol glucoside (7) from the ethyl acetate fraction of methanolic extract of the bark. compound 1 was screened for its antibacterial, antifungal and antioxidant potential. antibacterial and antifungal activities of the compound we ... | 2016 | 28000514 |
| recurrent episodes of candidemia due to candida glabrata, candida tropicalis and candida albicans with acquired echinocandin resistance. | mixed fungal infection and acquired echinocandin resistance of candida spp. remain infrequent. in this study we have reported the case of a patient hospitalized for tuberculosis who experienced multiple infections due to three common candida species (c. albicans, c. glabrata, c. tropicalis). furthermore, consecutive isolates from blood cultures and heart valve were found resistant to azoles (c. tropicalis) and to echinocandin with either novel (c. tropicalis) or previously described (c. albicans ... | 2016 | 27995055 |
| photoinactivation of single and mixed biofilms of candida albicans and non-albicans candida species using phorodithazine(®). | this study evaluated the effectiveness of antimicrobial photodynamic therapy (apdt) mediated by photodithazine(®) (pdz) formulated in hydrogel, in the inactivation of mono and duo-species biofilms of candida albicans, candida glabrata and candida tropicalis. standardized suspensions of each strain were prepared and after biofilm formation, mono-species were treated with 150 and 175mg/l of pdz for 20min (pre-irradiation time), and exposed to led light at a dose of 37.5j/cm(2) (660nm). the duo-spe ... | 2017 | 27988224 |
| a sensitive and a rapid multiplex polymerase chain reaction for the identification of candida species in concentrated oral rinse specimens in patients with diabetes. | oral candidiasis is being frequently recognized in patients with diabetes, and is associated with multiple pathogens including candida albicans, candida parapsilosis, candida glabrata and candida tropicalis. the aim of this study was to evaluate a usefulness of a multiplex polymerase chain reaction as a rapid diagnostic tool for identification of four oral candida pathogens in patients with diabetes. | 2017 | 27960572 |
| invitro antifungal susceptibilities of candida species to liposomal amphotericin b, determined using clsi broth microdilution, and amphotericin b deoxycholate, measured using the etest. | the antifungal susceptibilities of 598 isolates of candida spp. (bloodstream and other sterile sites) to liposomal amphotericin b (l-amb) versus amphotericin b (amb) were determined. mics were calculated using the clinical and laboratory standards institute broth microdilution (m27-a3) method for l-amb and the etest method for amb. the mic50/mic90 (µg ml-1) values for l-amb broth microdilution and amb etest were 0.25/1 and 0.19/0.5, respectively. the overall essential agreement (±2 dilutions) wa ... | 2017 | 27959780 |
| fungal cyp51 inhibitors vt-1161 and vt-1129 exhibit strong in vitro activity against candida glabrata and c. krusei isolates clinically resistant to azole and echinocandin antifungal compounds. | the in vitro activities of fungal cyp51 inhibitors vt-1161 and vt-1129 were determined for candida glabrata (n = 34) and c. krusei (n = 50). c. glabrata isolates were screened for fks gene mutations. all isolates were resistant clinically and/or in vitro to at least one standard antifungal compound. vt-1161 and vt-1129 mics for all isolates were at least 5-fold below achievable human plasma levels for vt-1161. vt-1161 and vt-1129 are promising for the treatment of resistant c. glabrata and c. kr ... | 2017 | 27956419 |
| identification and antifungal susceptibility testing of candida species: a comparison of vitek-2 system with conventional and molecular methods. | candida infection is a major cause of morbidity and mortality in immunocompromised patients; an accurate and early identification is a prerequisite need to be taken as an effective measure for the management of patients. the purpose of this study was to compare the conventional identification of candida species with identification by vitek-2 system and the antifungal susceptibility testing (ast) by broth microdilution method with vitek-2 ast system. | 2017 | 27942193 |
| migration and interaction tracking for quantitative analysis of phagocyte-pathogen confrontation assays. | invasive fungal infections are emerging as a significant health risk for humans. the innate immune system is the first line of defense against invading micro-organisms and involves the recruitment of phagocytes, which engulf and kill pathogens, to the site of infection. to gain a quantitative understanding of the interplay between phagocytes and fungal pathogens, live-cell imaging is a modern approach to monitor the dynamic process of phagocytosis in time and space. however, this requires the pr ... | 2017 | 27940225 |
| synergism between fluconazole and methylene blue-photodynamic therapy against fluconazole-resistant candida strains. | photodynamic therapy (pdt) has been proved to be effective against fungi and it may be employed as a coadjutant to conventional antifungal agents, leading to a more effective microbial control minimising side effects. this work evaluates the combined effect of pdt and fluconazole against resistant candida albicans, candida glabrata and candida krusei. the yeasts were submitted to methylene blue-pdt (mb-pdt) in sub-inhibitory concentrations. in the present work, mb-pdt combined with fluconazole w ... | 2017 | 27934831 |
| in vitro synergism of a water insoluble fraction of uncaria tomentosa combined with fluconazole and terbinafine against resistant non-candida albicans isolates. | uncaria tomentosa d.c. (rubiaceae) has several biological activities, including activity against resistant candida strains. the synergistic interaction with terbinafine or fluconazole can be an important alternative to overcome this resistance. | 2017 | 27931150 |
| an experimental modeling of trinomial bioengineering- crp, rdna, and transporter engineering within single cell factory for maximizing two-phase bioreduction. | a carbonyl reductase (cr) gene from candida glabrata cbs138 has been heterologously expressed in cofactor regenerating e. coli host to convert ethyl-4-chloro-3-oxobutanoate (cobe) into ethyl-4-chloro-3-hydroxybutanoate (chbe). the cr enzyme exhibited marked velocity at substrate concentration as high as 363mm with highest turnover number (112.77±3.95s(-1)). solitary recombineering of such catalytic cell reproduced chbe 161.04g/l per g of dry cell weight (dcw). introduction of combinatorially eng ... | 2017 | 27923567 |
| a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty. | to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka). | 2017 | 27919672 |
| candida species isolated from different body sites and their antifungal susceptibility pattern: cross-analysis of candida albicans and candida glabrata biofilms. | candida species are regular commensal in humans, but-especially in immunocompromised patients-they represent opportunistic pathogens giving rise to systemic infection. the aim of the present work was to isolate and characterize for their antifungal profile candida species from different body sites and to analyze the biofilms produced by c. albicans and c. glabrata isolates. eighty-one strains of candida species from 77 patients were identified. epidemiological study showed that the most isolated ... | 2016 | 27915303 |
| structural and functional elucidation of yeast lanosterol 14α-demethylase in complex with agrochemical antifungals. | azole antifungals, known as demethylase inhibitors (dmis), target sterol 14α-demethylase (cyp51) in the ergosterol biosynthetic pathway of fungal pathogens of both plants and humans. dmis remain the treatment of choice in crop protection against a wide range of fungal phytopathogens that have the potential to reduce crop yields and threaten food security. we used a yeast membrane protein expression system to overexpress recombinant hexahistidine-tagged s. cerevisiae lanosterol 14α-demethylase an ... | 2016 | 27907120 |
| promising therapies for fungal infection based on the study to elucidate mechanisms to cope with stress in candida species. | in recent years, the incidence of fungal infections has been increasing, particularly among patients with immune systems compromised by human immunodeficiency virus infection, organ transplantation, and/or chemotherapy for cancer. current therapies for treating systemic fungal infection have limited effectiveness and have created problems of adverse reactions and drug resistance. these issues therefore motivate us to develop novel antifungals. elucidation of stress response mechanisms and virule ... | 2016 | 27904062 |
| synthesis and antifungal screening of 2-{[1-(5-alkyl/arylalkylpyrazin-2-yl)ethylidene]hydrazono}-1,3-thiazolidin-4-ones. | two novel thiosemicarbazones and eight novel 2-{[1-(5-alkyl/arylalkylpyrazin-2-yl)ethylidene]hydrazono}-1,3-thiazolidin-4-ones were prepared and tested against a panel of eight fungal strains-candida albicans atcc 44859, candida tropicalis 156, candida krusei e 28, candida glabrata 20/i, trichosporon asahii 1188, aspergillus fumigatus 231, lichtheimia corymbifera 272, and trichophyton interdigitale 445. 1,3-thiazolidin-4-ones exhibited activity against all strains, the most potent derivative was ... | 2016 | 27886119 |
| candida glabrata pneumonia in a patient with chronic obstructive pulmonary disease. | pneumonia remains an important cause of morbidity and mortality among infectious diseases. streptococcus pneumoniae and viruses are the most common cause of pneumonia. candidiasis in such patients has been associated with haemodialysis, fungal colonization, exposure to broad-spectrum antibiotics, intensive care unit (icu) hospitalization, and immunocompromised patients. the most common cause of infection is c. albicans. the case presented here is of a 66-year-old male patient diagnosed with c. g ... | 2016 | 27882253 |
| the current treatment landscape: candidiasis. | the epidemiology of candida species infection has changed over recent decades, influenced by local hospital-related factors, patient predisposing conditions and type of antifungal agents administered. a shift from candida albicans as the predominant pathogen towards an increasing prevalence of the species candida glabrata and candida parapsilosis amongst critically ill patients has been documented. changes in candida species distribution may impact treatment recommendations due to differences in ... | 2016 | 27880665 |
| in vitro exposure to increasing micafungin concentrations easily promotes echinocandin resistance in candida glabrata isolates. | we assessed the in vitro susceptibility of five echinocandin-susceptible candida glabrata isolates after exposure to micafungin. the direct exposure to plates at different micafungin concentrations resulted in the inhibition of growth at 0.062 μg/ml. the progressive exposure was performed on plates using 0.031 μg/ml of micafungin and sequential propagation on plates containing the next 2-fold concentration; the mics of micafungin and anidulafungin increased sequentially, and all the isolates bec ... | 2017 | 27872063 |
| molecular characterization of candida isolates from intensive care unit patients, krakow, poland. | over the last decades, candida species have emerged as important pathogens in immunocompromised patients. nosocomial infections are mainly of endogenous origin. nevertheless, some cases of exogenous candidiasis have also been reported. | 2017 | 27864011 |
| echinocandin resistance in candida species isolates from liver transplant recipients. | liver transplant recipients are at risk of invasive fungal infections, especially candidiasis. echinocandin is recommended as prophylactic treatment but is increasingly associated with resistance. our aim was to assess echinocandin drug resistance in candida spp. isolated from liver transplant recipients treated with this antifungal class. for this, all liver-transplanted patients in a university hospital (créteil, france) between january and june of 2013 and 2015 were included. susceptibilities ... | 2017 | 27855078 |
| a comparative investigation of azole susceptibility in candida isolates from vulvovaginal candidiasis and recurrent vulvovaginal candidiasis patients in ghana. | vulvovaginal candidiasis (vvc) and recurrent vulvovaginal candidiasis (rvvc) affect millions of women and are typically treated with azoles. we know little about azole susceptibility of candida species from vvc versus rvvc patients, and nothing about african isolates. we have investigated the susceptibility of candida isolates from ghana to fluconazole, itraconazole and/or voriconazole. the percentage of candida albicans isolates showing susceptibility was significantly lower in rvvc than vvc pa ... | 2016 | 27838642 |
| molecular detection of resistance to echinocandins. | in the last years, life-threatening fungal diseases have increased significantly, due to the rising number of human individuals susceptible to fungal infections, which are in part complicated by the emergence of antifungal drug-resistant pathogens. among yeasts, candida albicans and candida glabrata are the most common organisms responsible for invasive fungal diseases. the molecular detection of echinocandin resistance in candida species may represent a useful means of monitoring the incidence ... | 2017 | 27837518 |
| t2 magnetic resonance for fungal diagnosis. | rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. t2candida panel is a novel qualitative diagnostic platform that was recently approved by the us food and drug administration (fda) for diagnosis of candidemia with a mean time to species identification of less than 5 h. t2candida panel is performed on the fully automated t2dx instrument in whole blood k2edta specimens and is able to detect ... | 2017 | 27837513 |
| p2x7 from j774 murine macrophages acts as a scavenger receptor for bacteria but not yeast. | we studied the effects of extracellular atp and ca(2+) on uptake of bacteria (staphylococcus aureus or escherichia coli) and live yeast (candida glabrata) by j774 macrophages to determine the role of endogenous p2x7 receptors in phagocytosis. our findings show that phagocytosis of bio-particles coated with s. aureus or e. coli was blocked by atp and the p2x7 receptor agonist bzatp, while yeast phagocytosis was not. a438079, an antagonist of p2x7 receptors, partially reverted the effects of atp o ... | 2016 | 27833023 |
| micafungin versus caspofungin in the treatment of candida glabrata infection: a case report. | micafungin and caspofungin, which are both echinocandins, elicit their antifungal effects by suppressing the synthesis of β-d-glucan, an essential component of fungal cell walls. if micafungin is not effective against a fungal infection, is it unreasonable to switch to caspofungin? | 2016 | 27821139 |
| nkp46 is an nk cell fungicidal pattern recognition receptor. | natural killer (nk) cells are an important contributor to innate host defense because of their role in direct microbial recognition and killing. vitenshtein et al. make an important contribution by demonstrating that nk cells kill candida glabrata using the nk activating receptor, nkp46, which recognizes the epa adhesins. | 2016 | 27816327 |
| the cghaa1-regulon mediates response and tolerance to acetic acid stress in the human pathogen candida glabrata. | to thrive in the acidic vaginal tract, candida glabrata has to cope with high concentrations of acetic acid. the mechanisms underlying c. glabrata tolerance to acetic acid at low ph remain largely uncharacterized. in this work, the essential role of the cghaa1 transcription factor (encoded by orf cagl0l09339g) in the response and tolerance of c. glabrata to acetic acid is demonstrated. transcriptomic analysis showed that cghaa1 regulates, directly or indirectly, the expression of about 75% of th ... | 2017 | 27815348 |
| transcriptional control of drug resistance, virulence and immune system evasion in pathogenic fungi: a cross-species comparison. | transcription factors are key players in the control of the activation or repression of gene expression programs in response to environmental stimuli. the study of regulatory networks taking place in fungal pathogens is a promising research topic that can help in the fight against these pathogens by targeting specific fungal pathways as a whole, instead of targeting more specific effectors of virulence or drug resistance. this review is focused on the analysis of regulatory networks playing a ce ... | 2016 | 27812511 |
| multiplex pcr designed to differentiate species within the candida glabrata complex. | no phenotypic methods are available to unequivocally differentiate species within the candida glabrata complex. | 2017 | 27810263 |