Publications

stereochemical course of hydrolysis catalysed by alpha-l-rhamnosyl and alpha-d-galacturonosyl hydrolases from aspergillus aculeatus.	the stereochemical course of hydrolysis catalysed by four aspergillus aculeatus enzymes acting on alpha-l-rhamnosyl and alpha-d-galacturonosyl linkages in the hairy regions of pectins has been determined using 1h-nmr. exogalacturonase acts with inversion of anomeric configuration (e-->a), shown by the initial release of beta-d-galpa from the non-reducing end of polygalacturonic acid. similarly, rhamnogalacturonan (rg) hydrolase also acts with inversion of anomeric configuration (e-->a) during hy ...	1998	9464254
molecular and phenotypic characterization of aspergillus japonicus and aspergillus aculeatus strains with special regard to their mitochondrial dna polymorphisms.	forty aspergillus japonicus and a. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. the rdnas proved to be invariable (even strains of the species a. aculeatus exhibited the same restriction profile), while the strains could be classified into seven different mtdna rflp groups. hybridisation data suggest that six of these mtdna types have certain common restriction sites, while mtdna type 7, which was exhibited by some a. aculea ...	1997	9442274
evidence that galactanase a from pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which e161 and e270 are the catalytic residues.	a genomic library of pseudomonas fluorescens subsp. cellulosa dna was screened for galactanase-positive recombinants. the nine galactanase positive phage isolated contained the same galactanase gene designated gala. the deduced primary structure of the enzyme (galactanase a; gala) encoded by gala had a mr of 42 130 and exhibited significant sequence identity with a galactanase from aspergillus aculeatus, placing gala in glycosyl hydrolase family 53. the enzyme displayed properties typical of an ...	1997	9398278
genetic immobilization of cellulase on the cell surface of saccharomyces cerevisiae.	we tried genetically to immobilize cellulase protein on the cell surface of the yeast saccharomyces cerevisiae in its active form. a cdna encoding fi-carboxymethylcellulase (cmcase) of the fungus aspergillus aculeatus, with its secretion signal peptide, was fused with the gene encoding the c-terminal half (320 amino acid residues from the c terminus) of yeast alpha-agglutinin a protein involved in mating and covalently anchored to the cell wall. the plasmid constructed containing this fusion gen ...	1997	9390459
cloning and characterization of a rhamnogalacturonan hydrolase gene from botrytis cinerea.	rhamnogalacturonan hydrolase (rgase a) cleaves alpha 1--2 linkages between rhamnosyl and galacturonosyl residues in pectin. a 1.9 kb rgase a cdna clone (bcrhga) was isolated from a b. cinerea cdna library using a pcr-amplified aspergillus aculeatus rgase a probe. it's 1.7 kb open reading frame had 62% identity at the amino acid level with a. aculeatus rgase a. northern blots of b. cinerea total rna probed with bcrhga revealed a 2 kb band, suggesting the cdna clone is full or nearly-full length. ...	1997	9385443
action patterns and mapping of the substrate-binding regions of endo-(1-->5)-alpha-l-arabinanases from aspergillus niger and aspergillus aculeatus.	the substrate binding sites of endo-(1-->5)-alpha-l-arabinanases (ec 3.2.1.99) from aspergillus niger and aspergillus aculeatus were investigated using reduced and regular (1-->5)-alpha-l-arabino-oligosaccharides and high performance anion exchange chromatographic analysis. calculation of bond cleavage frequencies and kcat/k(m) parameters for these substrates enabled the determination of the number of arabinofuranosyl binding subsites and the estimation of the binding affinities of each subsite. ...	1997	9352635
expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance.	expression cloning is a relatively new method for fast and efficient cloning of enzyme genes from fungi that are known to make complex enzyme mixtures. in contrast to traditional cloning methods that are usually dependent on knowledge of at least a partial amino acid sequence in order to synthesize appropriate dna probes or primers, the expression cloning method solely relies on access to reliable and sensitive enzyme assays. a representative expression cdna library is made in saccharomyces cere ...	1997	9299700
purification and characterization of an extracellular beta-glucosidase from the filamentous fungus acremonium persicinum and its probable role in beta-glucan degradation.	a beta-glucosidase from the culture filtrates of the filamentous fungus acremonium persicinum has been purified by (nh4)2so4 precipitation followed by anion-exchange and gel filtration chromatography. sds-page of the purified enzyme gave a single band with an apparent molecular mass of 128 kda. the enzyme is a monomeric protein with an isoelectric point of 4.3 and a ph optimum of 5.5. comparison of the n-terminal amino acid sequence revealed similarities between the a. persicinum enzyme and seve ...	1997	9291624
three neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases.	acetylesterase and cinnamoyl ester hydrolase activities were demonstrated in culture supernatant of the anaerobic ruminal fungus neocallimastix patriciarum. a cdna expression library from n. patriciarum was screened for esterases using beta-naphthyl acetate and a model cinnamoyl ester compound. cdna clones representing four different esterase genes (bnaa-d) were isolated. none of the enzymes had cinnamoyl ester hydrolase activity, but two of the enzymes (bnaa and bnac) had acetylxylan esterase a ...	1997	9274014
identification of a bacterial pectin acetyl esterase in erwinia chrysanthemi 3937.	erwinia chrysanthemi causes soft-rot diseases of various plants by enzymatic degradation of the pectin in plant cell walls. the structural complexity of pectin requires the combined action of several pectinases for its efficient breakdown. three types of pectinases have so far been identified in e. chrysanthemi: two pectin methyl esterases (pema, pemb), a polygalacturonase (pehx), and eight pectate lyases (pela, pelb, pelc, peld, pele, pell, pelz, pelx). we report in this paper the analysis of a ...	1997	9218776
cloning and characterization of two rhamnogalacturonan hydrolase genes from aspergillus niger.	a rhamnogalacturonan hydrolase gene of aspergillus aculeatus was used as a probe for the cloning of two rhamnogalacturonan hydrolase genes of aspergillus niger. the corresponding proteins, rhamnogalacturonan hydrolases a and b, are 78 and 72% identical, respectively, with the a. aculeatus enzyme. in a. niger cultures which were shifted from growth on sucrose to growth on apple pectin as a carbon source, the expression of the rhamnogalacturonan hydrolase a gene (rhga) was transiently induced afte ...	1997	9212401
an overview of the safety evaluation of the thermomyces lanuginosus xylanase enzyme (sp 628) and the aspergillus aculeatus xylanase enzyme (sp 578).	xylanases sp 628 and sp 578 were produced by submerged fermentation of aspergillus oryzae, containing a gene code originating from thermomyces lanuginosus and aspergillus aculeatus, respectively. both enzymes were subject to the same series of toxicological tests to document their safety in use. the enzymes are to be applied as processing aids in the baking industry and in wheat starch separation. neither enzyme was found to be mutagenic in the salmonella typhimurium reverse mutation assay, nor ...	1997	9205568
a putative rhamnogalacturonase required for sexual development of neurospora crassa.	in previous work, the asd-i (ascus development) gene of the filamentous fingus neurospora crassa was identified as a gene expressed preferentially during the sexual cycle and shown to be essential for normal sexual development. the asd-i gene has been sequenced and further characterized. it contains two introns, the first of which is in-frame and inefficiently or differentially spliced. the predicted asd-i protein has extensive homology with rhamnogalacturonase b of aspergillus aculeatus, which ...	1997	9178004
the crystal structure of rhamnogalacturonase a from aspergillus aculeatus: a right-handed parallel beta helix.	pectic substances are the major polysaccharide components of the middle lamella and primary cell wall of dicotyledonous plants. they consist of homogalacturonan 'smooth' regions and highly rhamnified 'hairy' regions of rhamnogalacturonan. the backbone in rhamnogalacturonan-l (rg-l), which is composed of alternating galacturonic acid and rhamnose residues, is the substrate for a new class of enzymes known as rhamnogalacturnoases (rgases). rgase a is a novel enzyme implicated in the enzymatic degr ...	1997	9115442
cloning and sequencing of the cdna encoding beta-glucosidase 1 from aspergillus aculeatus.	a cdna was isolated from an aspergillus aculeatus cdna library using synthetic oligodeoxyribonucleotide mixtures that corresponded to the internal amino acid (aa) sequence of mature beta-glucosidase 1 (bgl1). analysis of the nucleotide sequence of the cloned cdna insert revealed a 2580-bp open reading frame (orf) that encoded a 860-aa protein. the deduced aa sequence of the orf shared sequence similarity with several bgl from other microorganisms.	1996	8964516
stereochemical course of hydrolysis catalyzed by arabinofuranosyl hydrolases.	the stereochemical course of hydrolysis catalyzed by various enzymes acting on arabinofuranosyl linkages has been determined. 1h-nmr analysis of the action of endo-(1-->5)-alpha-l-arabinanases from aspergillus niger and aspergillus aculeatus showed that both hydrolyze linear arabinan with inversion of configuration, and may therefore act via a single displacement mechanism. this is consistent with the a. niger enzyme's classification in glycosyl hydrolase family 43. the catalytic mechanisms of a ...	1996	8946944
identification of regulatory mutants of aspergillus aculeatus affected in rhamnogalacturonan hydrolase expression.	rhamnogalacturonan hydrolase expression in a. aculeatus can be induced by pectin, but also by a combination of two constituent monosaccharides of pectin, rhamnose and galacturonic acid. the rhga promoter was fused to the a. niger glucose oxidase coding sequence and a single copy of the hybrid gene was integrated at the rhga locus in the genome of a. aculeatus. the gene product was subsequently used as reporter in a screening assay for the selection of rhamnogalacturonan hydrolase-overproducing m ...	1996	8929397
pectin methyl esterase from aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme.	seventeen full-length cdnas encoding pectin methyl esterase i (pme i) have been isolated from the filamentous fungus aspergillus aculeatus by expression cloning in yeast. yeast colonies expressing functional pme i were identified on agar plates containing highly esterified pectin, and a cdna encoding pme i was isolated. the deduced amino acid sequence of pme i is highly similar (74% identity) to the pme from aspergillus niger. a full-length cdna encoding pme i was cloned into an aspergillus expr ...	1996	8920970
the backbone of the pectic polysaccharide rhamnogalacturonan i is cleaved by an endohydrolase and an endolyase.	rhamnogalacturonan i (rg-i), a major pectic component of the primary walls of plant cells, is believed to play an important role in determining both the structure and functions of the walls. a more detailed structural description of rg-i is likely to lead to a greater understanding of the biological roles of this polysaccharide. two enzymes secreted by aspergillus aculeatus that have been cloned and expressed in a fungal system (kofod et al., j. biol. chem., 269, 29182-29189, 1994) cleave the rg ...	1995	8720076
rhamnogalacturonase b from aspergillus aculeatus is a rhamnogalacturonan alpha-l-rhamnopyranosyl-(1-->4)-alpha-d-galactopyranosyluronide lyase.	the recently described rhamnogalacturonase b, which is able to degrade ramified hairy regions of pectin, was found to be a rhamnogalacturonan alpha-l-rhamnopyranosyl-(1-->4)-alpha-d-galactopyranosyluronide lyase. the cleavage site and mechanism differ from that of the previously described rhamnogalacturonase a, which is a hydrolase and can now be termed rhamnogalacturonan alpha-d-galactopyranosyluronide-(1-->2)-alpha-l-rhamnopyranosyl hydrolase.	1996	8587995
crystallization and preliminary x-ray diffraction studies of an endoglucanase from aspergillus aculeatus.	most fungal cellulases are found in multiple forms varying in size and substrate specificity. aspergillus aculeatus is known to produce nine cellulolytic enzymes including an endoglucanase (fi cm-cellulase, m(r) = 24,002) as the major component. single crystals of fi cm-cellulase from aspergillus aculeatus have been prepared by sitting-drop vapour diffusion using ammonium sulphate as a precipitant. the cellulase crystals belong to the orthorhombic space group p2(1)2(1)2(1) with unit cell dimensi ...	1994	8057368
isolation and structural characterization of endo-rhamnogalacturonase-generated fragments of the backbone of rhamnogalacturonan i.	a combination of commercially available preparations of aspergillus niger beta-d-galactosidase, endo-alpha-l-arabinanase, alpha-l-arabinosidase, and endo-beta-d-galactanase has been used to generate oligoglycosyl fragments of the backbone of rhamnogalacturonan i (rg-i) that had been isolated from the walls of suspension-cultured sycamore cells. the backbone-cleaving enzyme, which is present in the beta-d-galactosidase preparation, only fragments the rg-i backbone when many of the neutral oligogl ...	1994	8001021
expression cloning, purification and characterization of a beta-1,4-mannanase from aspergillus aculeatus.	a cdna library from the filamentous fungus aspergillus aculeatus was constructed in the yeast expression vector pyes2.0 and used to isolate 57 full length cdna's encoding beta-1,4-mannanase by expression in s. cerevisiae. the positive clones were identified on agar plates containing 0.2% azurine dyed cross-linked mannan by the formation of blue halos around the colonies. all clones represented transcripts of the same mannanase gene (man1). the gene was sub-cloned into an aspergillus expression v ...	1994	7987261
rhamnogalacturonan alpha-l-rhamnopyranohydrolase. a novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin.	two alpha-l-rhamnohydrolases with different substrate specificities were isolated from a commercial preparation produced by aspergillus aculeatus. the first rhamnohydrolase was active toward p-nitrophenyl-alpha-l- rhamnopyranoside, naringin, and hesperidin and was termed p-nitrophenyl-alpha-l-rhamnopyranohydrolase (pnp-rhamnohydrolase). from the data collected, the enzyme seemed specific for the alpha-1,2- or alpha-1,6-linkage to beta-d-glucose. the pnp-rhamnohydrolase had a molecular mass of 87 ...	1994	7972516
cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from aspergillus aculeatus.	two rhamnogalacturonases from the filamentous fungus aspergillus aculeatus have been cloned and characterized. a cdna library from a. aculeatus was constructed, and a novel rhamnogalacturonase b was isolated by expression cloning in yeast. for this purpose a new plate screening assay was developed, specific for the detection of rhamnogalacturonase activity. the rhamnogalacturonase a, known from previous reports, was shown not to be expressed in yeast in an active form. therefore, rhamnogalacturo ...	1994	7961884
expression cloning, purification and characterization of a beta-1,4-galactanase from aspergillus aculeatus.	expression cloning has been used to isolate a cdna encoding beta-1,4-galactanase from the filamentous fungus aspergillus aculeatus. a cdna library was prepared from mycelia, inserted in a yeast expression vector and transformed into saccharomyces cerevisiae. thirteen clones secreting galactanase activity were identified from a screening of approximately 2.5 x 10(4) yeast colonies. all clones expressed transcripts of the same galactanase gene. the cdna was re-cloned in an aspergillus expression v ...	1995	7788716
expression of the cellulase (fi-cmcase) gene of aspergillus aculeatus in saccharomyces cerevisiae.	as a step to breed a saccharomyces cerevisiae strain able to produce ethanol directly from cellulose, we combined cdna for aspergillus aculeatus fi-cmcase (fi-carboxymethyl cellulase) with the gap (glyceraldehyde-3-phosphate dehydrogenase) promoter of s. cerevisiae and used the resultant plasmid, pyec91, to transform s. cerevisiae. the transformed cells produced active fi-cmcase within the cytoplasm. western-blot analysis following sds-polyacrylamide gel electrophoresis demonstrated that the cel ...	1994	7764982
expression of the cellulase (fi-cmcase) gene of aspergillus aculeatus in escherichia coli.	fi-cmcase cdna of aspergillus aculeatus was expressed in escherichia coli by using the tac promoter of e. coli. transformants of e. coli harboring a plasmid phem06 containing mature form fi-cmcase cdna produced fi-cmcase in the cytoplasm of the cells. the enzyme from e. coli cells was purified to yield 56% and it was immunological identical to that of fi-cmcase purified from a. aculeatus.	1993	7764343
molecular cloning and characterization of a rhamnogalacturonan acetylesterase from aspergillus aculeatus. synergism between rhamnogalacturonan degrading enzymes.	a rhamnogalacturonan acetylesterase (rgae) was purified to homogeneity from the filamentous fungus aspergillus aculeatus, and the nh2-terminal amino acid sequence was determined. full-length cdnas encoding the enzyme were isolated from an a. aculeatus cdna library using a polymerase chain reaction-generated product as a probe. the 936-base pair rha1 cdna encodes a 250-residue precursor protein of 26,350 da, including a 17-amino acid signal peptide. the rha1 cdna was overexpressed in aspergillus ...	1995	7592973
cloning and sequencing of cellulase cdna from aspergillus kawachii and its expression in saccharomyces cerevisiae.	the cdna encoding the endo-beta-1,4-glucanase (carboxymethylcellulase; cmcase-i) from aspergillus kawachii ifo 4308 was cloned. nucleotide-sequence analysis of the cloned cdna insert showed a 717-bp open reading frame that encoded a protein of 239 amino-acid residues. the predicted amino-acid sequence of the mature protein had considerable homology with the protein sequence of the fi-cmcase of aspergillus aculeatus. the cdna was introduced into saccharomyces cerevisiae. the expressed enzyme had ...	1995	7586029
cloning, sequence and expression of the gene coding for rhamnogalacturonase of aspergillus aculeatus; a novel pectinolytic enzyme.	rhamnogalacturonase was purified from culture filtrate of aspergillus aculeatus after growth in medium with sugar-beet pulp as carbon source. purified protein was used to raise antibodies in mice and with the antiserum obtained a gene coding for rhamnogalacturonase (rhga) was isolated from a lambda cdna expression library. the cloned rhga gene has an open-reading frame of 1320 base pairs encoding a protein of 440 amino acids with a predicted molecular mass of 45 962 da. the protein contains a po ...	1995	7576553
cellulolytic enzymes associated with the fruit rots of citrus sinensis caused by aspergillus aculeatus and botryodiplodia theobromae.	botryodiplodia theobromae and aspergillus aculeatus were inoculated in carboxymethylcellulose (cmc) medium and on filter papers. hydrolysis of the cmc medium and degradation of the filter papers were observed, indicating the production of c1 and cx cellulases by the two rot pathogens. the c1 and cx enzymes were also detected in filtrates of rotted orange fruits obtained by infection with the two pathogens. the cellulases could not induce rot development on their own. however, when they were adde ...	1983	6624142
a possible new pathogenic aspergillus isolation and general mycological properties of the fungus.	a species of aspergillus was isolated from vomitus and scrapings of the tongue of a patient with a form of respiratory illness. the fungus has since been identified as aspergillus aculeatus, iizuka. the fungus grew over a wide range of temperatures, the spores appeared to be thermophilic. many local foodstuffs supported the growth of the fungus in culture. ultraviolet light inhibited mycelia growth and sporulation of a. aculeatus. the fungicides brestan, benlate, fundazole and kocide 101 inhibit ...	1984	6099970
intracellular localization of nigeran in the wall of aspergillus aculeatus by autoradiography with the electron microscope.	aspergillus aculeatus mycelium was incubated with [(3)h]glucose under conditions that promote nigeran (mycodextran) accumulation (low ph, in the absence of nitrogen). autoradiography revealed that essentially all the (3)h was localized around the hyphal perimeter. the results strongly support a hyphal wall location for nigeran.	1974	4829925
regulation of nigeran accumulation by aspergillus aculeatus.	nigeran (mycodextran), an unbranched glucan consisting of alternating alpha,1-4 and alpha,1-3 linkages, is accumulated intracellularly by aspergillus aculeatus, under conditions of nitrogen limitation and low ph (optimum ph 5.0). both conditions are necessary. nigeran is synthesized de novo from exogenous glucose and accounts for about 20% of the glucose transported by the mycelium. the polymer is diluted out but not degraded when the mycelium is transferred to fresh medium containing nh(4) (+). ...	1973	4690967
cloning and sequence analysis of a cdna for cellulase (fi-cmcase) from aspergillus aculeatus.	we have cloned and characterized the cdna coding for a major component of cellulase, endoglucanase (fi-cmcase), produced by aspergillus aculeatus. the cdna was isolated from a a. aculeatus cdna library using synthetic oligonucleotide mixtures that correspond to the internal amino acid sequence of the mature fi-cmcase protein. nucleotide sequence analysis of the cloned cdna insert revealed a 711 bp open reading frame that encoded a protein of 237 amino acid residues. the primary structure of fi-c ...	1990	2249253
complete nucleotide sequence of a gene coding for aspergillus aculeatus cellulase (fi-cmcase).		1990	2216782
purification of an aspartic proteinase from aspergillus aculeatus.		1991	1812714
studies on aculeacin. ii. isolation and characterization of aculeacins b, c, d, e, f and g.	six new antibiotics were isolated as the minor components related to aculeacin a from the culture broth of aspergillus aculeatus m-4214 and named as aculeacins b, c, d, e, f and g. their physico-chemical properties were analogous to those of aculeacin a and they showed significant activity against fungi. all of the minor components liberated palmitic acid on alkaline hydrolysis. amino acid analysis showed that threonine and hydroxyproline are common constituents of aculeacins.	1977	863789
secalonic acids d and f are toxic metabolites of aspergillus aculeatus.		1977	830866
biosynthetic relationships among the secalonic acids. isolation of emodin, endocrocin and secalonic acids from pyrenochaeta terrestris and aspergillus aculeatus.	cynodontin, emodin, endocrocin and secalonic acids a, e and g have been isolated from five strains of pyrenochaeta terrestis. aspergillus aculeatus produces emodin, endocrocin and secalonic acids b, d and f. no cynodontin was detected. isolation of emodin in small amounts supports previous evidence that it is an intermediate in secalonic acid biosynthesis. isolation of cynodontin and endocrocin, which are co-produced with secalonic acids in other organisms, suggests that these compounds are form ...	1979	541252
the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.	a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...	1979	471028
studies on aculeacin. i. isolation and characterization of aculeacin a.	aculeacin a, a new antifungal antibiotic was isolated from the mycelial cake of aspergillus aculeatus m-4214. the antibiotic is a white amorphous powder soluble in lower alcohols and hardly soluble in other organic solvents or water. aculeacin a gave palmitic acid and five ninhydrin-positive products including theonine, hydroxyproline upon acid hydrolysis. the antibiotic showed a potent activity against molds and yeasts, but exhibited no antibacterial activity. aculeacin a has relatively low tox ...	1977	324959