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comparative analysis of type iii secreted effector genes reflects divergence of acidovorax citrulli strains into three distinct lineages.acidovorax citrulli causes bacterial fruit blotch of cucurbits, a serious economic threat to watermelon (citrullus lanatus) and melon (cucumis melo) production worldwide. based on genetic and biochemical traits, a. citrulli strains have been divided into two distinct groups: group i strains have been mainly isolated from various non-watermelon hosts, while group ii strains have been generally isolated from and are highly virulent on watermelon. the pathogen depends on a functional type iii secre ...201424848275
efficient production of transgenic melon via agrobacterium-mediated transformation.oriental melon (cucumis melo l. var. makuwa) is an important fruit for human consumption. however, this plant species is one of the most recalcitrant to genetic transformation. the lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for oriental melon. in this study, an efficient transgenic production method for agrobacterium-mediated transformation using cotyledon explants of oriental melon was developed. cotyledon explants were pre-cult ...201424841654
left atrium the size of a honeydew melon. 201424792279
new chromone and triglyceride from cucumis melo seeds.re-investigation of the meoh extract of the seeds of cucumis melo l. var. reticulatus (cucurbitaceae) led to the isolation of a new chromone derivative (5,7- dihydroxy-2-[2-(3-methoxy-4-hydroxyphenyl)ethyl]chromone (5) and a triglyceride (1,3-di-(6z,9z)-docosa-6,9-dienoyl-2-(6z) hexacos-6-enoylglycerol (1), together with three known compounds; alpha-spinasterol (2), stigmasta-7,22,25-trien-3-ol (3), and d:b-friedoolean-5-ene-3-beta-ol (4), are reported from this species for the first time. their ...201424689290
assessing transmission of crop diseases by insect vectors in a landscape context.theory indicates that landscape composition affects transmission of vector-borne crop diseases, but few empirical studies have investigated how landscape composition affects plant disease epidemiology. since 2006, bemisia tabaci (gennadius) has vectored the cucurbit yellow stunting disorder virus (cysdv) to cantaloupe and honeydew melons (cucumis melo l.) in the southwestern united states and northern mexico, causing significant reductions in yield of fall melons and increased use of insecticide ...201424665679
characterization of conserved micrornas from five different cucurbit species using computational and experimental analysis.micrornas (mirnas) are ∼21 nt non-coding small rnas which regulate gene expression at the post-transcriptional level in plants and animals. until recently, only limited numbers of mirnas were identified in cucurbitaceae, a large flowering plant family. in this study, 220 potential mirna candidates were identified from five species of cucurbitaceae family using a comparative genome-based computational analysis. a comprehensive bioinformatic analysis of est (expressed sequence tag) and gss (genomi ...201424657600
inducement of mitosis delay by cucurbitacin e, a novel tetracyclic triterpene from climbing stem of cucumis melo l., through gadd45γ in human brain malignant glioma (gbm) 8401 cells.cucurbitacin e (cue) is a natural compound previously shown to have anti-feedant, antioxidant and antitumor activities as well as a potent chemo-preventive action against cancer. the present study investigates its anti-proliferative property using mtt assay; cue demonstrated cytotoxic activity against malignant glioma gbm 8401 cells and induced cell cycle g2/m arrest in these cells. cue-treated cells accumulated in metaphase (cue 2.5-10 μm) as determined using mpm-2 by flow cytometry. we attempt ...201424577085
investigation of a food-borne salmonella oranienburg outbreak in a mexican prison.gastroenteritis outbreaks in prisons represent a public health risk worldwide. identifying and characterizing the etiological agents of gastroenteritis outbreaks in prisons is important for implementing effective prevention and infection control measures. we present the first studied case of a gastroenteritis outbreak in a mexican prison.201424518623
increase in femaleness of three cucurbits by treatment with ethrel, an ethylene releasing compound.the application of ethrel (2-chloroethane phosphonic acid), an ethylene-releasing compound, to monoecious cultivars of cucumber and squash and an andromonoecious cultivar of muskmelon, caused a shift towards femaleness in all three species. the increase in femaleness manifested itself in several symptoms: a decrease in the number of staminate (male) flowers, an increase in the number of pistillate (female) or hermaphrodite (perfect) flowers, and a change in flowering pattern, namely, formation o ...196924515743
phenotypic profiling and gene expression analyses for aromatic and volatile compounds in chamoes (cucumis melo).gotgam chamoe (ggc), a native oriental melon in korea, is known to possess the aroma of a dried persimmon, an agronomic relevance for melon breeding program. the volatile compounds and the transcript levels of aromatic compound genes in cultivar (ohbokggul chamoe [oc]) and ggc were profiled. a total of 62 volatile compounds were identified and quantified. twenty-eight volatile compounds were specific to either the oc or the ggc. the amounts of volatile alcohol, saturated hydrocarbon, and unsatur ...201424515385
correlation and path coefficient analyses in sweet melon (cucumis melo var. aegyptiacus l.) under irrigated and drought conditions.genotypic correlation and path analyses were carried out for growth, yield and fruit quality traits in 13 sweet melon genotypes collected from different places in egypt. seeds of these melon populations were sown under irrigated and drought stress conditions. the analysis of variance for the studied traits showed that the differences among genotypes were highly significant for all studied traits under irrigation and drought stress. under irrigated conditions, total yield per plant was positively ...201324505983
[effects of elevated rhizosphere co2 concentration on the photosynthetic characteristics, yield, and quality of muskmelon].by using aeroponics culture system, this paper studied the effects of elevated rhizosphere co2 concentration on the leaf photosynthesis and the fruit yield and quality of muskmelon during its anthesis-fruiting period. in the fruit development period of muskmelon, as compared with those in the control (350 microl co2 x l (-1)), the leaf chlorophyll content, net photosynthetic rate (pn), stomatal conductance (gs), intercellular co2 concentration (ci), and the maximal photochemical efficiency of ps ...201324483082
isolation and identification of the gibberellins of cucumis sativus and cucumis melo.thin-layer chromatography, gas-liquid chromatography, and mass spectrometry were used to identify gibberellins isolated from mature seeds of both cucumis sativus (cucumber) and cucumis melo (muskmelon). the gibberellins were extracted and purified by organic solvent fractionation, paper and thin-layer chromatography, and crystallization. seeds of c. sativus were found to contain gibberellins a1, a3, a4, and a7 with a1 the predominant species. seeds of c. melo contained gibberellins a1 and a3 and ...197224481557
screening suitable reference genes for normalization in reverse transcription quantitative real-time pcr analysis in melon.melon (cucumis melo. l) is not only an economically important cucurbitaceous crop but also an attractive model for studying many biological characteristics. screening appropriate reference genes is essential to reverse transcription quantitative real-time pcr (rt-qpcr), which is key to many studies involving gene expression analysis. in this study, 14 candidate reference genes were selected, and the variations in their expression in roots and leaves of plants subjected to biotic stress, abiotic ...201424475250
an efficient regeneration protocol for agrobacterium-mediated transformation of melon (cucumis melo l.).an efficient selection and plant regeneration protocol for agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. the new agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. the transformation system was maximized to maintain the integrity of melon itself, thus avoiding the limitations of traditional tissue culture methods. the transformation was carried out un ...201424446287
a model for transport of a viral membrane protein through the early secretory pathway: minimal sequence and endoplasmic reticulum lateral mobility requirements.viral movement proteins exploit host endomembranes and the cytoskeleton to move within the cell via routes that, in some cases, are dependent on the secretory pathway. for example, melon necrotic spot virus p7b, a type ii transmembrane protein, leaves the endoplasmic reticulum (er) through the copii-dependent golgi pathway to reach the plasmodesmata. here we investigated the sequence requirements and putative mechanisms governing p7b transport through the early secretory pathway. deletion of eit ...201424438546
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...197624424985
a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ...197624424601
metabolomics in melon: a new opportunity for aroma analysis.cucumis melo fruit is highly valued for its sweet and refreshing flesh, however the flavour and value are also highly influenced by aroma as dictated by volatile organic compounds (vocs). a simple and robust method of sampling vocs on polydimethylsiloxane (pdms) has been developed. contrasting cultivars of c. melo subspecies melo were investigated at commercial maturity: three cultivars of var. cantalupensis group charentais (cv. cézanne, escrito, and dalton) known to exhibit differences in ripe ...201424417788
sensory and instrumental analysis of medium and long shelf-life charentais cantaloupe melons (cucumis melo l.) harvested at different maturities.the flavour profiles of two genotypes of charentais cantaloupe melons (medium shelf-life and long shelf-life), harvested at two distinct maturities (immature and mature fruit), were investigated. dynamic headspace extraction (dhe), solid-phase extraction (spe), gas chromatography-mass spectrometry (gc-ms) and gas chromatography-olfactometry/mass spectrometry (gc-o/ms) were used to determine volatile and semi-volatile compounds. qualitative descriptive analysis (qda) was used to assess the organo ...201424262549
the cucumis plastome: physical map, intrageneric variation and phylogenetic relationships.a restriction map of the cucumis melo l. (melon) plastome was constructed by using several mapping approaches: single and double digestions of the chloroplast dna (chldna) with endonucleases (xhoi, smai, saci and pvuii) and hybridization to heterologous chldna probes and to isolated melon chldna fragments. four plastome-coded genes were located using heterologous probes. the overall organization and gene position of the melon plastome was found to be similar to that of tobacco and other angiospe ...198524247447
in vitro plant regeneration from leaf and cotyledon explants of cucumis melo l.five different genotypes from in vitro as well as greenhouse grown melon plants were shown to be highly responsive for in vitro shoot formation from leaf explants when placed on basic ms medium supplemented with 1 mg/l 6-benzylaminopurine. in addition, a very suitable regeneration system was obtained when cotyledon pieces of mature seeds were incubated on the same culture medium. in this case, the first shoots already appeared after 10 days of incubation, and hundreds of shoots were formed on th ...198924240446
regeneration of plants from leaf explants of cucumis melo cv. pusa sharbati.leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of cucumis melo were cultured on a ms medium supplemented with a range and combination of growth regulators. maximum shoot differentiation from the leaf explants occurred in the combined presence of bap and 2ip at equimolar concentration of 1 μm. regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. for elongation the shoots were transferred to ms+bap [1 μm] ...198824240267
are sucrosyl-oligosaccharides synthesized in mesophyll protoplasts of mature leaves of cucumis melo?biosynthesis of sucrosyl-oligosaccharides (raffinose, stachyose) was traced in source leaves of cucumis melo after (14)c-photoassimilation. the main carbon compound exported was (14)c-labeled stachyose. no oligosaccharide synthesis was detected in young, importing leaves. mesophyll protoplasts, isolated from mature leaves which had previously photosynthesized (14)co2, did not contain (14)c-oligosaccharides but contained [(14)c]-sucrose and (14)c-hexoses. isolated minor-vein-enriched fractions fr ...198624232761
pathway of assimilate transfer between mesophyll cells and minor veins in leaves of cucumis melo l.photoassimilating mature leaves of cucumis melo exported carbon at a rate of 1.7 mg c·dm(-2)·h(-1). radiolabeling with (14)c showed that stachyose and raffinose are the main carbohydrates translocated. autoradiograms indicated that sieve elements of the abaxial phloem of minor veins are the sole conduits for carbon export from mature leaves and carbon import into immature leaflets. sieve elements of the abaxial phloem are associated with intermediary cells which are intimately connected with the ...198724227267
variability and correlations in muskmelon in relation to the cultivation method.six fruit characters have been measured in 23 cultivars of cucumis melo, representing a wide geographical range. plants were grown both in the greenhouse and in the field. when the 23 cultivars were analyzed together, the largest component of variance was found between cultivars under both growth conditions, suggesting the existence of large genetic diversity for all the characters studied. generally, variance between plants within cultivars was less than or equal to variance between fruits with ...198924227250
plant regeneration from cotyledon protoplasts of xinjiang muskmelon.cotyledon protoplasts were isolated from seedlings of xinjiang muskelon (cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified miller medium. high frequency division of the protoplast-derived cells was observed. agarose bead culture with b6s3 tobacco crown gall nurse cells was found most suitable for the cotyledon protoplasts when compared with other culture methods. intact plants were regenerated from the protocalli by a two-step culture procedure with liquid and ...199024226702
agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants.transgenic muskmelon (cucumis melo l.) plants were produced efficiently by inoculating cotyledon explants with agrobacterium tumefaciens strain lba4404 bearing a ti plasmid with the npt ii gene for kanaymcin resistance. after co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. shoot regeneration occurred within 3-5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. morpho ...199024226603
regulation of organogenesis and somatic embryogenesis by auxin in melon, cucumis melo l.various tissues of seeds and seedlings of melon were cultured in vitro to study the effects of auxin concentration on organogenesis and embryogenesis. adventitious shoots and somatic embryos were formed from explants of cotyledons of mature seeds, hypocotyls of seedlings, and leaves and petioles of young plantlets. expanded cotyledons of seedlings formed only adventitious shoots. all tissues responded similarly to the 2,4-d concentration in the media, that is, adventitious shoots were formed at ...199124221584
high presence/absence gene variability in defense-related gene clusters of cucumis melo.changes in the copy number of dna sequences are one of the main mechanisms generating genome variability in eukaryotes. these changes are often related to phenotypic effects such as genetic disorders or novel pathogen resistance. the increasing availability of genome sequences through the application of next-generation massive sequencing technologies has allowed the study of genomic polymorphisms at both the interspecific and intraspecific levels, thus helping to understand how species adapt to ...201324219589
evaluation of restriction fragment length polymorphism in cucumis melo.the objectives of this study were to assess the degree of restriction fragment length polymorphism (rflp) in cucumis melo and to determine interrelationships among cultivated varieties. initial screening of a genomic psti library revealed that approximately 40% of the clones were repetitive. a total of 162 unique and low-copy sequence clones were hybridized to seven diverse accesions of c. melo and a c. sativus cultivar 'pacer' to evaluate rflp variation. of these, 130 probes (80%) detected a po ...199224202522
induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (cucumis melo l.).a procedure leading to the regeneration of whole plants from protoplasts of melon is described. protoplasts were isolated from cotyledons and leaves of plants grown in vitro. after 14 days of culture, average viability and division rates were respectively 60% and 30% for the two organs, considering total initial protoplasts plated. the manipulation of the exogenous auxin / cytokinin balance in regeneration media enabled to direct morphogenesis towards somatic embryogenesis (1 mg·l(-1) 2,4-dichlo ...199224201728
tolerance to a whitefly-transmitted virus causing muskmelon yellows disease in spain.muskmelon yellowing disease was one of the most serious problems affecting muskmelon crops along the south-east coast of spain throughout the 1980s. the causal agent of this disease is a virus that we call muskmelon yellows virus (myv); myv is transmitted by the greenhouse whitefly trialeurodes vaporariorum westwood. it has proven impossible to find sources of resistance to myv within a wide collection of spanish muskmelon landraces and exotic varieties. however, 'nagata kin makuwa' and pi 16137 ...199224201359
stachyose synthesis in mature leaves of cucumis melo. purification and characterization of stachyose synthase (ec 2.4.1.67).galactinol: raffinose-6-galactosyltransferase (ec 2.4.1.67), a stachyose synthase, was extracted from mature leaves of cucumis melo cv. ranjadew and was purified to homogeneity by (nh4)2so4 precipitation, ion-exchange chromatography, gel-filtration and non-denaturing polyacrylamide gel electrophoresis. a specific activity of 516 μkat · mg(-1) and a 160-fold purification was achieved. the ph optimum of the enzyme reaction was found to be 6.8 in sodium-phosphate buffer, and the temperature optimum ...199124194243
differential accumulation of hydroxyproline-rich glycoproteins in bean root nodule cells infected with a wild-type strain or a c4-dicarboxylic acid mutant of rhizobium leguminosarum bv. phaseoli.an antiserum raised against deglycosylated hydroxyproline-rich glycoproteins (hpgps) from melon (cucumis melo l.) was used to study the relationship between rhizobium infection and induction of hrgps in bean (phaseolus vulgaris l.) root nodule cells infected with either the wild-type or a c4-dicarboxylic acid mutant strain of rhizobium leguminosarum bv. phaseoli. in effective nodules, where fixation of atmospheric dinitrogen is taking place, hrgps were found to accumulate mainly in the walls of ...199124194237
agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro).cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ...199424193640
ploidy of somatic embryos and the ability to regenerate plantlets in melon (cucumis melo l.).the number of chromosomes in cells of callus, somatic embryos and regenerated plantlets during somatic embryogenesis were examined in two cultivars of melon (cucumis melo l.). somatic embryos were diploid (50.0%/32.1%), tetraploid (38.5%/57.5%) and octoploid (11.5%/10.4%) whereas in callus cells diploidy (41.9%/43.3%), tetraploidy (27.9%/25.8%), octoploidy (11.6%/15.5%) and a low frequency of other types of ploidy and aneuploidy were observed. mixoploid somatic embryos were not observed. these r ...199424192875
selection of somaclonal variants with low-temperature germinability in melon (cucumis melo l.).plants were regenerated from adventitious buds and somatic embryos (r0) of melon (cucumis melo l.), the cultivar andes. somaclonal variants of melon with low temperature germinability were selected from the progenies (r1) of r0 plants. among 5,618 r1 seeds harvested from 23 r0 plants that were regenerated from adventitious buds 4 seeds germinated after 5 days of culture at 15 °c (selection rate; 0.07%). however, among 374 r2 seeds harvested from 2 r1 plants no seed germinated after 7 days of cul ...199524186622
distribution and immunolocalization of stachyose synthase in cucumis melo l.indirect evidence for the site of stachyose biosynthesis has been provided by determining the occurrence and distribution of stachyose, raffinose and galactinol, the donor of the galactosyl moiety for stachyose synthesis, in cucumis melo l. cv. ranjadew. studies of enzyme activities for the synthesis of these sugars and their distribution in different plant organs and isolates has led to the conclusion that stachyose is synthesized mainly in mature leaves and seeds. nevertheless, stachyose-synth ...199124186524
[estimation model for daily transpiration of greenhouse muskmelon in its vegetative growth period].for developing an estimation method of muskmelon transpiration in greenhouse, an estimation model for the daily transpiration of greenhouse muskmelon in its vegetative growth period was established, based on the greenhouse environmental parameters, muskmelon growth and development parameters, and soil moisture parameters. according to the specific environment in greenhouse, the item of aerodynamics in penman-monteith equation was modified, and the greenhouse environmental sub-model suitable for ...201324175525
length polymorphism and homologies of microsatellites in several cucurbitaceae species.the objectives of this research were to assess (1) the degree of simple sequence repeats (ssr) dna length polymorphism in melon (cucumis melo l.) and other species within the cucurbitaceae family and (2) the possibility of utilizing ssrs flanking primers from single species to other genera or species of cucurbitaceae. five melon (ct/ga) n ssrs were isolated from a genomic library. two cucumber (cucumis sativus l.) ssrs were detected through a search of dna sequence databases, one contained a (ct ...199624162541
a genetic map of melon (cucumis melo l.) with rflp, rapd, isozyme, disease resistance and morphological markers.one hundred and ten markers were analysed for linkage in 218 f2 plants derived from two divergent cultivars ('védrantais' and 'songwhan charmi') of cucumis melo (l.). thirty-four rflps, 64 rapds, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cm of the melon genome. rapd and rflp markers detected similar polymorphism levels. rflps were largely due to base substitutions rather than insertion/d ...199624162199
evaluation of levulinic acid and sodium dodecyl sulfate as a sanitizer for use in processing georgia-grown cantaloupes.freshly harvested georgia-grown cantaloupes (cucumis melo l. var. reticulatus cv. athena and atlantis) were spot inoculated with 100 μl of a five-strain mixture of salmonella enterica serovar poona (9 log cfu/ml) at the stem scar and on the netted rind and then subjected to no treatment (control) or a 6-min treatment (tank only) in water, 120 ppm of chlorine (ph 7.0), 1% levulinic acid plus 0.1% sodium dodecyl sulfate (sds; ph 3.0), or 2% levulinic acid plus 0.2% sds (ph 3.0). the log reduction ...201324112578
development of ssr markers by next-generation sequencing of korean landraces of chamoe (cucumis melo var. makuwa).the oriental melon (cucumis melo var. makuwa), called 'chamoe' in korean, is a popular fruit crop cultivated mainly in asia and a high-market value crop in korea. to provide molecular breeding resources for chamoe, we developed and characterized genomic ssr markers from the preliminary illumina read assemblies of gotgam chamoe (one of the major landraces; km) and sw3 (the breeding parent). mononucleotide motifs were the most abundant type of markers, followed by di-, tri-, tetra-, and pentanucle ...201324096890
effect of high oxygen and high carbon dioxide atmosphere packaging on the microbial spoilage and shelf-life of fresh-cut honeydew melon.this study evaluated the potential of modified atmospheres (mas) combining high oxygen (o₂) and high carbon dioxide (co₂) levels to extend the shelf-life of fresh-cut honeydew melon. firstly, the effect of ma on the growth and volatile organic metabolite production of candida sake, leuconostoc mesenteroides and leuconostoc gelidum, which had all been previously isolated from spoiled commercial fresh-cut honeydew melon, was evaluated separately on honeydew melon agar at 7 °c. additionally, the ef ...201324021822
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...201324015567
occurrence of fuligo gyrosa causing slime mold of oriental melon.recently, a severe slime mold infestation affected oriental melon plants in fields in chilgok county, gyeongbuk province, korea. specimens were collected from the fields and examined for identification. a species of myxomycetes, fuligo gyrosa, was identified based on its morphological characteristics. this is the first report that f. gyrosa causes slime mold of oriental melon.200923983540
occurrence of fruit rot of melon caused by sclerotium rolfsii in korea.in 2007 to 2008, a fruit rot of melon (cucumis melo l.) caused by sclerotium rolfsii occurred sporadically in a farmer's vinyl house in jinju city. the symptoms started with watersoaking lesion and progressed into the rotting of the surface of fruit. white mycelial mats appeared on the lesion at the surface of the fruit and a number of sclerotia formed on the fruit near the soil line. the sclerotia were globoid in shape, 1~3 mm in size, and white to brown in color. the hyphal width was measured ...200923983527
genetic and chemical characterization of an ems induced mutation in cucumis melo crtiso gene.in order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'charentais' type melon line that accumulates β-carotene. one mutagenized m2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofi'). hplc analysis revealed that 'yofi' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. the altered carotenoid composition of 'yofi' is associated with a significant change of the ...201323973661
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
taxonomic significance of trichomes micromorphology in cucurbits.studies on trichomes micromorphology using scanning electron microscope (sem) were undertaken in 23 species with one variety under 13 genera of the family cucurbitaceae (viz., benincasa hispida (thunb.) cogn., citrullus lanatus (thunb.) matsum. & nakai, cucumis melo var. agrestis naudin, cucumis sativus l., diplocyclos palmatus (l.) c. jeffrey, edgaria dargeelingensis c.b. clarke, gynostemma burmanicum king ex chakr., gynostemma pentaphyllum (thunb.) makino, gynostemma pubescens (gagnep.) c.y. w ...201123961108
soft rot on cucumis melo var. makuwa caused by rhizopus oryzae.rhizopus oryzae is reported for the first time on cucumis melo l. var. makuwa makino. a detailed description of this korean specimen is given, along with its rdna internal transcribed spacer sequence. on the basis of mycological characteristics and molecular data, the fungus was identified as r. oryzae went & prinsen geerligs.201023956677
identification and characterization of the causal organism of gummy stem blight in the muskmelon (cucumis melo l.).gummy stem blight is a major foliar disease of muskmelon (cucumis melo l.). in this study, morphological characteristics and rdna internal transcribed spacer (its) sequences were analyzed to identify the causal organism of this disease. morphological examination of the jeonbuk isolate revealed that the percentage of monoseptal conidia ranged from 0% to 10%, and the average length × width of the conidia was 70 (± 0.96) × 32.0 (± 0.15) µm on potato dextrose agar. the blast analysis showed nucleoti ...201023956648
synthesis of 9-oxononanoic acid, a precursor for biopolymers.polymers based on renewable resources have become increasingly important. the natural functionalization of fats and oils enables an easy access to interesting monomeric building blocks, which in turn transform the derivative biopolymers into high-performance materials. unfortunately, interesting building blocks of medium-chain length are difficult to obtain by traditional chemical means. herein, a biotechnological pathway is established that could provide an environmentally suitable and sustaina ...201323934656
induction of antiviral responses by acibenzolar-s-methyl against cucurbit chlorotic yellows virus in melon.cucurbit chlorotic yellows virus (ccyv) (family closteroviridae, genus crinivirus) is an emerging virus which causes severe diseases on melon (cucumis melo) plants. ccyv-infected melon plants display yellowing, mottling, chlorosis, or chlorotic spots on leaves. to develop a new control strategy, the potential for 1,2,3-benzothiadiazole-7-thiocarboxylic acid-s-methyl-ester (asm) to suppress ccyv infection was evaluated. asm treatment on melon plants greatly increased the expression levels of path ...201323927428
genetic and virulence variability among erwinia tracheiphila strains recovered from different cucurbit hosts.the causal agent of cucurbit bacterial wilt, erwinia tracheiphila, has a wide host range in the family cucurbitaceae, including economically important crops such as muskmelon (cucumis melo), cucumber (c. sativus), and squash (cucurbita spp.). genetic variability of 69 e. tracheiphila strains was investigated by repetitive-element polymerase chain reaction (rep-pcr) using boxa1r and eric1-2 primers. fingerprint profiles revealed significant variability associated with crop host; strains isolated ...201323927426
vision loss following intraocular listeriosis associated with contaminated cantaloupe.intraocular listeriosis, a rare manifestation of invasive listeriosis, has a poor visual prognosis. we report an intraocular listeriosis case related to a multistate outbreak associated with contaminated cantaloupe. increasing awareness of rare listeriosis presentations might facilitate timely diagnosis and treatment, and case reporting can clarify medical and epidemiologic aspects of listeriosis.201323898285
evaluation of an analytical methodology using quechers and gc-sq/ms for the investigation of the level of pesticide residues in brazilian melons.a multiresidue method based on the sample preparation by modified quechers and detection by gas chromatography coupled to single quadruple mass spectrometers (gc-sq/ms) was used for the analysis of 35 multiclass pesticides in melons (cucumis melo inodorus) produced in ceara-brazil. the rates of recovery for pesticides studied were satisfactory (except for the etridiazole), ranging from 85% to 117% with a relative standard deviation (rsd) of less than 15%, at concentrations between 0.05 and 0.20 ...201323871010
the suitability of three galia melon cultivars and different types of cuts for the fresh-cut industry.melons (cucumis melo l.) are widely cultivated in mediterranean countries. for fresh-cut melon elaboration, factors such as type of cultivar and cut have a significant impact on final product quality. the quality parameters from long, medium and short shelf-life galia melon cultivars, cut into different shapes such as slices, trapezoids and cylinders and stored up to 10 days at 5 °c, were evaluated in order to learn the suitability of these cultivars and type of cut for minimal processing.201323860939
diversification and genetic differentiation of cultivated melon inferred from sequence polymorphism in the chloroplast genome.molecular analysis encouraged discovery of genetic diversity and relationships of cultivated melon (cucumis melo l.). we sequenced nine inter- and intra-genic regions of the chloroplast genome, about 5500 bp, using 60 melon accessions and six reference accessions of wild species of cucumis to show intra-specific variation of the chloroplast genome. sequence polymorphisms were detected among melon accessions and other cucumis species, indicating intra-specific diversification of the chloroplast g ...201323853513
assessment of a 1h high-resolution magic angle spinning nmr spectroscopy procedure for free sugars quantification in intact plant tissue.in most plants, sucrose is the primary product of photosynthesis, the transport form of assimilated carbon, and also one of the main factors determining sweetness in fresh fruits. traditional methods for sugar quantification (mainly sucrose, glucose and fructose) require obtaining crude plant extracts, which sometimes involve substantial sample manipulation, making the process time-consuming and increasing the risk of sample degradation. here, we describe and validate a fast method to determine ...201323824526
comparison of different methods for separation of haploid embryo induced through irradiated pollen and their economic analysis in melon (cucumis melo var. inodorus).irradiated pollen technique is the most successful haploidization technique within cucurbitaceae. after harvesting of fruits pollinated with irradiated pollen, classical method called as "inspecting the seeds one by one" is used to find haploid embryos in the seeds. in this study, different methods were used to extract the embryos more easily, quickly, economically, and effectively. "inspecting the seeds one by one" was used as control treatment. other four methods tested were "sowing seeds dire ...201323818825
a simple epidural simulator: a blinded study assessing the 'feel' of loss of resistance in four fruits.complex epidural simulators are now available, but these are expensive and not widely available. simple simulators using fruit have been described before.201323749185
[identification of two fusarium isolates and their crude toxin allelopathic effect on cucumis melo seedlings].eight fungi isolates were obtained from fusarium-infected cucumis melo (melon) plants and their rhizosphere soils. taking melon cultivar 'xitian 1' as test material, the re-inoculation and seed germination experiments were conducted to investigate the pathogenicity and growth inhibition effect of these fungi isolates on melon. through the determination of the induced enzyme activities, resistant substance contents, and cell membrane permeability of potted melon roots, the allelopathic effect of ...201323718002
characterization and chemical composition of fatty acids content of watermelon and muskmelon cultivars in saudi arabia using gas chromatography/mass spectroscopy.the growth in the production of biodiesel, which is principally fatty acid methyl esters (fame), has been phenomenal in the last ten years because of the general desire to cut down on the release of greenhouse gases into the atmosphere, and also as a result of the increasing cost of fossil fuels.201323661995
biological and molecular characterization of two distinct tomato strains of cucumber mosaic virus based on complete rna-3 genome and subgroup specific diagnosis.cucumber mosaic virus (cmv) is one of the most important viral pathogen infecting several plant species in india. five isolates of cmv obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in india were analysed based on host-reactions and genome sequence. the majority of the isolates were very similar and only two isolates, tfr-in and tss-in showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (cp) gene. tfr-in was isolated ...201123637513
chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india.bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ...201023637479
[a case report with esophageal foreign body of muskmelon].a male patient, 41 years old, swallowed a chunk of muskmelon because his attention was not centralized. odynophagia and dysphagia were the two main clinical manifestations. the esophagus barium swallow examination showed a foreign body in the upper esophagus. the patient did the rigid esophagoscope examination in the operating room under general anesthesia. there was a chunk of muskmelon in the upper esophagus and could not take it out from the esophagus by using foreign body forceps because the ...201323631144
myrotoxins from a plant pathogenic isolate ofmyrothecium roridum.an isolate ofm roridum (atcc 52485) which is a potent pathogen to muskmelon was shown to produce a series of macrocyclic trichothecenes, the myrotoxins, heretofore found to be produced only by an. isolate ofm roridum which is a pathogen to tomato. the fact that these two isolates are virulent pathogens and both produce the same potent mycotoxins suggests that these novel trichothecenes may play an important role in the pathogenicity of the fungi.199123605653
penetration, post-penetration development, and reproduction of meloidogyne incognita on cucumis melo var. texanus.cucumis melo var. texanus, a wild melon commonly found in the southern united states and two accessions, burleson co. and mx 1230, expressed resistance to meloidogyne incognita in preliminary experiments. to characterize the mechanism of resistance, we evaluated root penetration, post-penetration development, reproduction, and emigration of m. incognita on these two accessions of c. melo var. texanus. additionally, we evaluated 22 accessions of c. melo var. texanus for their reaction against m. ...201323589661
flavour profiles of three novel acidic varieties of muskmelon (cucumis melo l.).novel acidic varieties of muskmelon (cucumis melo l.) are emerging onto the uk market. these melons contain almost twice the amount of citric acid compared to standard melons and are described as 'zesty and fresh'. this study compared the flavour components of three acidic varieties with a standard galia-type melon. the volatile and semivolatile compounds were extracted, using dynamic headspace extraction (dhe) or solid-phase microextraction (spme) and solid phase extraction (spe), respectively, ...201323561221
an integrated approach for flavour quality evaluation in muskmelon (cucumis melo l. reticulatus group) during ripening.numerous and diverse physiological changes occur during fruit ripening and maturity at harvest is one of the key factors influencing the flavour quality of fruits. the effect of ripening on chemical composition, physical parameters and sensory perception of three muskmelon (cucumis melo l. reticulatus group) cultivars was evaluated. significant correlations emerging from this extensive data set are discussed in the context of identifying potential targets for melon sensory quality improvement. a ...201323561094
the application of high-concentration short-time chlorine dioxide treatment for selected specialty crops including roma tomatoes (lycopersicon esculentum), cantaloupes (cucumis melo ssp. melo var. cantaloupensis) and strawberries (fragaria×ananassa).the effects of high-concentration short-time chlorine dioxide (clo2) gas treatment on food-borne pathogens inoculated onto the surface of tomatoes, cantaloupes, and strawberries were studied. produce were spot-inoculated with a mixture of salmonella enterica (serotypes montevideo, javiana and baildon), escherichia coli o157:h7 (serotypes 204 p, edl 933 and c792) or listeria monocytogenes (serotypes scott a, f 5069 and lcdc 81-861), and treated with clo2 gas at 10 mg/l for 180 s. after clo2 gas t ...201323541196
purification, cdna cloning and recombinant protein expression of a phloem lectin-like anti-insect defense protein bplp from the phloem exudate of the wax gourd, benincasa hispida.latex and other exudates in plants contain various proteins that are thought to play important defensive roles against herbivorous insects and pathogens. herein, the defensive effects of phloem exudates against the eri silkworm, samia ricini (saturniidae, lepidoptera) in several cucurbitaceous plants were investigated. it was found that phloem exudates are responsible for the defensive activities of cucurbitaceous plants, such as the wax gourd benincasa hispida and cucumis melo, especially in b. ...201323453909
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...201123431109
the fate of murine norovirus and hepatitis a virus during preparation of fresh produce by cutting and grating.human noroviruses and hepatitis a virus (hav) are commonly associated with outbreaks occurring in restaurant establishments and catered events. food handlers are major contributing factors to foodborne illnesses initiated in the kitchen setting. in this study, transfer of hav and murine norovirus (mnv-1), a human norovirus surrogate, between produce (cucumbers, strawberries, tomatoes, cantaloupes, carrots, and honeydew melons) and common kitchen utensils (graters and knives) was investigated. th ...201223412721
a major latex-like protein is a key factor in crop contamination by persistent organic pollutants.this is the first report, to our knowledge, to reveal important factors by which members of the cucurbitaceae family, such as cucumber (cucumis sativus), watermelon (citrullus lanatus), melon (cucumis melo), pumpkin (cucurbita pepo), squash (c. pepo), and zucchini (c. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides and dioxins. xylem sap of c. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hy ...201323404917
catabolism of l-methionine in the formation of sulfur and other volatiles in melon (cucumis melo l.) fruit.sulfur-containing aroma volatiles are important contributors to the distinctive aroma of melon and other fruits. melon cultivars and accessions differ in the content of sulfur-containing and other volatiles. l-methionine has been postulated to serve as a precursor of these volatiles. incubation of melon fruit cubes with ¹³c- and ²h-labeled l-methionine revealed two distinct catabolic routes into volatiles. one route apparently involves the action of an l-methionine aminotransferase and preserves ...201323402686
[nondestructive measurement of sugar content of hami melon based on diffuse reflectance hyperspectral imaging technique].the research on nondestructive test for detecting the sugar content of hami melon by the technology of hyperspectral imaging was put forward. the research used the hyperspectral imaging system to get the diffuse reflective spectrum information (400 - 1 000 nm) of anilox class hami melon sugar content, chose effective whole wavelength (500 - 820 nm)to do the modeling regression analysis the sugar content of hami melon. the research compared the correction method of msc and snv, and also compared ...201223387186
snp genotyping in melons: genetic variation, population structure, and linkage disequilibrium.novel sequencing technologies were recently used to generate sequences from multiple melon (cucumis melo l.) genotypes, enabling the in silico identification of large single nucleotide polymorphism (snp) collections. in order to optimize the use of these markers, snp validation and large-scale genotyping are necessary. in this paper, we present the first validated design for a genotyping array with 768 snps that are evenly distributed throughout the melon genome. this customized illumina goldeng ...201323381808
physical removal and transfer of murine norovirus and hepatitis a virus from contaminated produce by scrubbing and peeling.human noroviruses and hepatitis a virus are responsible for numerous outbreaks associated with handling fresh produce. in this study, physical removal of hepatitis a virus and murine norovirus, a human norovirus surrogate, from contaminated produce items (honeydew melons, cantaloupes, carrots, and celery) by scrubbing under running water with a nylon brush or scouring pad and by peeling (carrots and celery) with a peeler was investigated. the degree and extent of utensil contamination with virus ...201323317861
genetic diversity among melon accessions (cucumis melo) from turkey based on ssr markers.melon (cucumis melo) is an important vegetable crop in turkey, where it is grown in many regions; the most widely planted lines are local winter types belonging to the var. inodorous. we examined 81 melon genotypes collected from different provinces of turkey, compared with 15 reference melon genotypes obtained from inra/france, to determine genetic diversity among turkish melons. twenty polymorphic primers were used to generate the ssr markers. pcr amplification was performed and electrophoresi ...201223315810
structural analysis of the alcohol acyltransferase protein family from cucumis melo shows that enzyme activity depends on an essential solvent channel.alcohol acyltransferases (aat) play a key role in ester biosynthesis. in cucumis melo var. cantalupensis, aats are encoded by a gene family of four members (cmaat1-4). cmaat1, cmaat3 and cmaat4 are capable of synthesizing esters, with cmaat1 the most active. cmaat2 is inactive and has an ala268 residue instead of a threonine which is present in all other active aats, although the role of this residue is still unclear. the present work aims to understand the molecular mechanism involved in ester ...201323311868
dna fingerprinting of chinese melon provides evidentiary support of seed quality appraisal.melon, cucumis melo l. is an important vegetable crop worldwide. at present, there are phenomena of homonyms and synonyms present in the melon seed markets of china, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. molecular markers, especially microsatellites or simple sequence repeats (ssrs) are playing increasingly important roles for cultivar identification. the aim of this study was to construct a dna fingerpri ...201223285039
isolation, characterization, and expression analysis of cmmlo2 in muskmelon.the full-length cdna sequence of the mlo gene was cloned via smart-race-pcr from muskmelon (cucumis melo l.), and was designated as cmmlo2 (genbank accession no. fj713542). the gene is 1,710 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. localization analysis in onion epidermal cells showed that cmmlo2-gfp is localized in the plasma membrane. the expression of cmmlo2 gene was analyzed in melon leaf infected with po ...201323238921
identification of early response genes to salt stress in roots of melon (cucumis melo l.) seedlings.in order to better understand the mechanisms by which muskmelons (cucumis melo l.) respond to salt stress, a cdna library was constructed using suppression subtractive hybridization (ssh) from the root tissue of a salt-tolerant melon cultivar, bingxuecui. a total of 339 clones were sequenced from the ssh library, leading to 312 high quality expressed sequence tags (ests), with an average size of 450 bp; representing 262 uni-ests comprising 29 contigs and 233 singletons. blast analysis of the ded ...201323212618
removal of pb2+ from aqueous solution by adsorption on chemically modified muskmelon peel.a cost-effective biosorbent was prepared by a green chemical modification process from muskmelon peel by saponification with alkaline solution of ca(oh)2. its adsorption behavior for lead ions was investigated and found to exhibit excellent adsorption properties. results showed that the optimal equilibrium ph range for 100% adsorption is from 4 up to 6.4. adsorption equilibrium was attained within 10 min. the adsorption process can be described well by langmuir model and pseudo-second-order kine ...201323212270
expression of small rna in aphis gossypii and its potential role in the resistance interaction with melon.the regulatory role of small rnas (srnas) in various biological processes is an active area of investigation; however, there has been limited information available on the role of srnas in plant-insect interactions. this study was designed to identify srnas in cotton-melon aphid (aphis gossypii) during the vat-mediated resistance interaction with melon (cucumis melo).201223173035
phytochemical and in vitro total antioxidant capacity analyses of peel extracts of different cultivars of cucumis melo and citrullus lanatus.cucumis melo linn. (cucurbitaceae) and citrullus lanatus thunb. (cucurbitaceae) are desert vegetables popular for their nutritional value and year round availability. the pulp and seeds of these plants are used for dietary purposes or as medications for certain ailments in the folk medicinal system. peels of the fruit are either wasted or used as feed of grazing animals.201323153254
root transcriptional responses of two melon genotypes with contrasting resistance to monosporascus cannonballus (pollack et uecker) infection.monosporascus cannonballus is the main causal agent of melon vine decline disease. several studies have been carried out mainly focused on the study of the penetration of this pathogen into melon roots, the evaluation of symptoms severity on infected roots, and screening assays for breeding programs. however, a detailed molecular view on the early interaction between m. cannonballus and melon roots in either susceptible or resistant genotypes is lacking. in the present study, we used a melon oli ...201223134692
plectosphaerella species associated with root and collar rots of horticultural crops in southern italy.plectosphaerella cucumerina, most frequently encountered in its plectosporium state, is well known as a pathogen of several plant species causing fruit, root and collar rot, and collapse. it is considered to pose a serious threat to melon (cucumis melo) production in italy. in the present study, an intensive sampling of diseased cucurbits as well as tomato and bell pepper was done and the fungal pathogens present on them were isolated. phylogenetic relationships of the isolates were determined t ...201223105152
clinical efficacy of a novel topical formulation for vitiligo: compared evaluation of different treatment modalities in 149 patients.current vitiligo treatments are not always satisfactory for both patients and dermatologists. recently, combination therapies have been introduced in order to obtain better results and reduce risks in the management of the disease. novel efficacious products are needed to improve the therapeutic possibilities of dermatologists in the respect of safety for the patients. the objective of the present study was to evaluate the effects of a novel topical in a gel formulation containing phenylalanine, ...201323046028
anti-hyperlipidemic activity of cucumis melo fruit peel extracts in high cholesterol diet induced hyperlipidemia in rats.abstract cucumis melo linn. (cucurbitaceae) fruits have been used, traditionally in indian traditional system of medicine, for the treatment of various disorders such as liver tonic, cardioprotective, antidiabetic, antiobesity, etc. the aim of the present study was to investigate the possible anti-hyperlipidemic activity of cucumis melo fruit peel (cmfp) methanolic and aqueous extract in high cholesterol diet induced hyperlipidemia in rats. treatment with cmfp methanolic and aqueous extract show ...201223023565
electronic detection of drechslera sp. fungi in charentais melon ( cucumis melo naudin) using carbon-nanostructure-based sensors.the development of chemical sensor technology in recent years has stimulated an interest regarding the use of characteristic volatiles and odors as a rapid and early indication of deterioration in fruit quality. the fungal infestation by drechslera sp. in melons is a severe problem, and we demonstrate that electronic sensors based on carbon nanostructures are able to detect the presence of these fungi in melon. the responses of sensor conductance g and capacitance c at 27 khz were measured and u ...201223003336
verification of aroma profiles of jiashi muskmelon juice characterized by odor activity value and gas chromatography-olfactometry/detection frequency analysis: aroma reconstitution experiments and omission tests.to verify the aroma profile of jiashi muskmelon previously identified by gas chromatography-olfactometry/detection frequency analysis (gc-o/dfa) and odor activity value (oav) calculation, the synthetic blends of odorants (aroma models) were prepared and then were compared with the original jiashi muskmelon juice aroma using quantitative descriptive analysis (qda) and electronic nose analysis (ena), respectively. qda and ena both indicated that the model solution derived from oav calculation more ...201222970770
comparative morphology of minute intestinal fluke eggs that can occur in human stools in the republic of korea.the egg morphology of minute intestinal flukes (mif) that can occur as human infections in the republic of korea, i.e., metagonimus yokogawai, m. miyatai, m. takahashii, heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus, stictodora fuscata, pygidiopsis summa, and gymnophalloides seoi, was studied in comparison with clonorchis sinensis. the adult worms were obtained from residents of endemic areas, and their intrauterine eggs were studied and measured using light microscopy; t ...201222949747
phloem-mobile aux/iaa transcripts target to the root tip and modify root architecture.in plants, the phloem is the component of the vascular system that delivers nutrients and transmits signals from mature leaves to developing sink tissues. recent studies have identified proteins, mrna, and small rna within the phloem sap of several plant species. it is now of considerable interest to elucidate the biological functions of these potential long-distance signal agents, to further our understanding of how plants coordinate their developmental programs at the whole-plant level. in thi ...201222925478
cholecystolithiasis is associated with clonorchis sinensis infection.the objective of this study was to analyze gallbladder stones for direct evidence of a relationship between clonorchis sinensis infection and gallbladder stones formation.201222905137
high efficiency joint cze determination of sugars and acids in vegetables and fruits.in this work, an improved ce method for the medium-throughput determination of main organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose, glucose and sucrose in several food matrices is described. these compounds have been identified as key components in the taste intensity of fruit and vegetable crops. using a running buffer with 20 mm 2,6-pyridine dicarboxylic acid ph 12.1 and 0.1% hexadimethrine bromide, replacing it every 5 h to avoid ph decrease, and op ...201222887163
crystal structure of cucumisin, a subtilisin-like endoprotease from cucumis melo l.cucumisin is a plant serine protease, isolated as an extracellular glycoprotein from the melon fruit cucumis melo l. var. prince. cucumisin is composed of multiple domain modules, including catalytic, protease-associated, and fibronectin-iii-like domains. the crystal structure of cucumisin was determined by the multiwavelength anomalous dispersion method and refined at 2.75å resolution. a structural homology search indicated that the catalytic domain of cucumisin shares structural similarity wit ...201222841692
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