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identification and evidence of positive selection upon resistance gene analogs in cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as fusarium oxysporum f. sp. vasinfectum (fov) causing severe wilt disease. resistance gene analogs (rgas) are the largest class of potential resistance (r) genes depicting highly conserved domains and structures in plants. additionally, rgas are pivotal components of breeding projects towards host disease resistance, serving as useful functional mark ...201627729728
a raf-like mapkkk gene, ghraf19, negatively regulates tolerance to drought and salt and positively regulates resistance to cold stress by modulating reactive oxygen species in cotton.mitogen-activated protein kinase kinase kinases (mapkkks) function at the top level of mapk cascades and play important roles in plant development and stress responses. although mapkkks comprise the largest family in the mapk cascades, very few raf-like mapkkks have been functionally identified, especially in the economically important crop cotton. in this study, a raf-like mapkkk gene, ghraf19, was characterized for the first time in cotton. our data show that the expression of ghraf19 was inhi ...201627717463
characterization of eleven monosomic alien addition lines added from gossypium anomalum to gossypium hirsutum using improved gish and ssr markers.gossypium anomalum (bb genome) possesses the desirable characteristics of drought tolerance, resistance to diseases and insect pests, and the potential for high quality fibers. however, it is difficult to transfer the genes associated with these desirable traits into cultivated cotton (g. hirsutum, aadd genome). monosomic alien addition lines (maals) can be used as a bridge to transfer desired genes from wild species into g. hirsutum. in cotton, however, the high number and smaller size of the c ...201627717331
ghabf2, a bzip transcription factor, confers drought and salinity tolerance in cotton (gossypium hirsutum l.).the bzip transcription factor (tf) act as an important regulator for the abscisic acid (aba) mediated abiotic stresses signaling pathways in plants. here, we reported the cloning and characterization of ghabf2, encoding for typical cotton bzip tf. overexpression of ghabf2 significantly improved drought and salt stress tolerance both in arabidopsis and cotton. however, silencing of ghabf2 made transgenic cotton sensitive to peg osmotic and salt stress. expression of ghabf2 was induced by drought ...201627713524
na(+) compartmentalization related to salinity stress tolerance in upland cotton (gossypium hirsutum) seedlings.the capacity for ion compartmentalization among different tissues and cells is the key mechanism regulating salt tolerance in plants. in this study, we investigated the ion compartmentalization capacity of two upland cotton genotypes with different salt tolerances under salt shock at the tissue, cell and molecular levels. we found that the leaf glandular trichome could secrete more salt ions in the salt-tolerant genotype than in the sensitive genotype, demonstrating the excretion of ions from ti ...201627698468
genome-wide identification and expression analysis of stress-associated proteins (saps) containing a20/an1 zinc finger in cotton.stress-associated proteins (saps) containing the a20/an1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. nevertheless, few studies have focused on the sap gene family in cotton. to explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of saps in tetraploid gossypium hirsutum l. tm-1 (ad1). a total of 37 genes encoding saps were identified, 36 of which were duplicated in the ...201627681253
ghcam7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production.calcium signaling regulates many developmental processes in plants. calmodulin (cam) is one of the most conserved calcium sensors and has a flexible conformation in eukaryotes. the molecular functions of cam are unknown in cotton, which is a major source of natural fiber. in this study, a gossypium hirsutum l.cam7-like gene was isolated from upland cotton. bioinformatics analysis indicated that the ghcam7-like gene was highly conserved as compared with arabidopsis atcam7. the ghcam7-like gene sh ...201627669397
impact of insect management on population dynamics and insecticide resistance of tarnished plant bug (hemiptera: miridae).while transgenic plants targeting lepidopteran and coleopteran insects have been available for almost 20 yr, there are no transgenic crops that target hemipteran insects such as tarnished plant bug, lygus lineolaris (palisot de beauvois), though at least one company lists potential products in advanced stages of development. a resistance management model for the u.s. mid-south was developed to aid in resistance risk assessments for transgenic crops targeting l. lineolaris, and validated against ...201627651293
genome-wide identification and functional analysis of the tify gene family in response to drought in cotton.jasmonates control many aspects of plant biological processes. they are important for regulating plant responses to various biotic and abiotic stresses, including drought, which is one of the most serious threats to sustainable agricultural production. however, little is known regarding how jasmonate zim-domain (jaz) proteins mediate jasmonic acid signals to improve stress tolerance in cotton. this represents the first comprehensive comparative study of tify transcription factors in both diploid ...201627640194
histone modifications define expression bias of homoeologous genomes in allotetraploid cotton.histone modifications regulate gene expression in eukaryotes, but their roles in gene expression changes in interspecific hybrids or allotetraploids are poorly understood. histone modifications can be mapped by immunostaining of metaphase chromosomes at the single cell level and/or by chromatin immunoprecipitation-sequencing (chip-seq) for analyzing individual genes. here, we comparatively analyzed immunostained metaphase chromosomes and chip-seq of individual genes, which revealed a chromatin b ...201627637746
ghmcs1, the cotton orthologue of human grim-19, is a subunit of mitochondrial complex i and associated with cotton fibre growth.grim-19 (gene associated with retinoid-interferon-induced mortality 19) is a subunit of mitochondrial respiratory complex i in mammalian systems, and it has been demonstrated to be a multifunctional protein involved in the cell cycle, cell motility and innate immunity. however, little is known about the molecular functions of its homologues in plants. here, we characterised ghmcs1, an orthologue of human grim-19 from cotton (gossypium hirsutum l.), and found that it was essential for maintaining ...201627632161
enhanced plant growth promoting role of phycomolecules coated zinc oxide nanoparticles with p supplementation in cotton (gossypium hirsutum l.).this report focuses on application of zinc oxide nanoparticles (znonps) carrying phycomolecule ligands as a novel plant growth promoter aimed at increasing the crop productivity. the present investigation examined the effect of znonps on plant growth characteristics, and associated biochemical changes in cotton (gossypium hirsutum l.) following growth in a range of concentrations (25-200 mg l(-l) znonps) in combination with 100 mm p in a hydroponic system. treated plants registered an increase i ...201727622847
metabolic engineering of cottonseed oil biosynthesis pathway via rna interference.cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (vitamine) as well as unsaturated fatty acid. however, the cottonseed oil content of cultivated cotton (gossypium hirsutum) is only around 20%. in this study, we modified the accumulation of oils by the down-regulation of phosphoenolpyruvate carboxylase 1 (ghpepc1) via rna interference in transgenic cotton plants. the qrt-pcr and enzyme activity ass ...201627620452
saccharibacillus endophyticus sp. nov., an endophyte of cotton.a gram-positive-staining, facultatively aerobic, endospore-forming bacterial strain, isolated from the stem tissue of cotton (gossypium hirsutum) was subjected to a detailed taxonomic study using a polyphasic approach. based on 16s rrna gene sequence similarity comparisons, strain jm-1350t grouped into the genus saccharibacillus, and was most closely related to the type strain of saccharibacillus sacchari (96.5 % 16s rrna gene sequence similarity), followed by the type strains of saccharibacillu ...201627613514
genome-wide characterization and expression analysis of myb transcription factors in gossypium hirsutum.myb family proteins are one of the most abundant transcription factors in the cotton plant and play diverse roles in cotton growth and evolution. previously, few studies have been conducted in upland cotton, gossypium hirsutum. the recent release of the g. hirsutum genome sequence provides a great opportunity to identify and characterize the entire upland cotton myb protein family.201627613381
identification of chromosome segment substitution lines of gossypium barbadense introgressed in g. hirsutum and quantitative trait locus mapping for fiber quality and yield traits.chromosome segment substitution lines mbi9804, mbi9855, mbi9752, and mbi9134, which were obtained by advanced backcrossing and continuously inbreeding from an interspecific cross between ccri36, a cultivar of upland cotton (gossypium hirsutum) as the recurrent parent, and hai1, a cultivar of sea island cotton (g. barbadense) as the donor parent, were used to construct a multiple parent population of (mbi9804×mbi9855)×(mbi9752×mbi9134). the segregating generations of double-crossed f1 and f2 and ...201627603312
lightweight males of podisus nigrispinus (heteroptera: pentatomidae) neglect lightweight females due low reproductive fitness.sexual choice by male stink bugs is important because females that experience food shortages lay fewer eggs with lower viability compared with well-fed females. in this study, we investigated whether podisus nigrispinus (dallas) (heteroptera: pentatomidae) males fed with a low-quality diet during its nymphal stage show selectivity for sexual partners resulting in high-quality progeny. lightweight males and females were obtained from nymphs fed weekly with tenebrio molitor l. (coleoptera: tenebri ...201627599098
cloning and functional analysis of the promoter of an ascorbate oxidase gene from gossypium hirsutum.apoplastic ascorbate oxidase (ao) plays significant roles in plant cell growth. however, the mechanism of underlying the transcriptional regulation of ao in gossypium hirsutum remains unclear. here, we obtained a 1,920-bp promoter sequence from the gossypium hirsutum ascorbate oxidase (ghao1) gene, and this ghao1 promoter included a number of known cis-elements. promoter activity analysis in overexpressing pghao1::gfp-gus tobacco (nicotiana benthamiana) showed that the ghao1 promoter exhibited h ...201627597995
identification and characterization of the ghhsp20 gene family in gossypium hirsutum.in higher plants, heat shock protein 20 (hsp20) plays crucial roles in growth, development and responses to abiotic stresses. in this study, 94 ghhsp20 genes were identified in g. hirsutum, and these genes were phylogenetically clustered into 14 subfamilies. out of these, 73 paralogous gene pairs remained in conserved positions on segmental duplicated blocks and only 14 genes clustered into seven tandem duplication event regions. transcriptome analysis showed that 82 ghhsp20 genes were expressed ...201627580529
identification of favorable snp alleles and candidate genes for traits related to early maturity via gwas in upland cotton.early maturity is one of the most important and complex agronomic traits in upland cotton (gossypium hirsutum l). to dissect the genetic architecture of this agronomically important trait, a population consisting of 355 upland cotton germplasm accessions was genotyped using the specific-locus amplified fragment sequencing (slaf-seq) approach, of which a subset of 185 lines representative of the diversity among the accessions was phenotypically characterized for six early maturity traits in four ...201627576450
the ghtt2_a07 gene is linked to the brown colour and natural flame retardancy phenotypes of lc1 cotton (gossypium hirsutum l.) fibres.some naturally coloured brown cotton fibres from accessions of gossypium hirsutum l. can be used to make textiles with enhanced flame retardancy (fr). several independent brown fibre loci have been identified and mapped to chromosomes, but the underlying genes have not yet been identified, and the mechanism of lint fibre fr is not yet fully understood. in this study, we show that both the brown colour and enhanced fr of the lc1 lint colour locus are linked to a 1.4mb inversion on chromosome a07 ...201627567364
insights into interspecific hybridization events in allotetraploid cotton formation from characterization of a gene-regulating leaf shape.the morphology of cotton leaves varies considerably. phenotypes, including okra, sea-island, super-okra, and broad leaf, are controlled by a multiple allele locus, l2 okra leaf (l2°) is an incomplete mutation that alters leaf shape by increasing the length of lobes with deeper sinuses. using a map-based cloning strategy, we cloned the l2 locus gene, which encodes a late meristem identity 1 (lmi1)-like transcription factor (ghokra). silencing ghokra leads to a change in phenotype from okra to bro ...201627558139
characterization and functional analysis of pebp family genes in upland cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is a naturally occurring photoperiod-sensitive perennial plant species. however, sensitivity to the day length was lost during domestication. the phosphatidylethanolamine-binding protein (pebp) gene family, of which three subclades have been identified in angiosperms, functions to promote and suppress flowering in photoperiod pathway. recent evidence indicates that pebp family genes play an important role in generating mobile flowering signals. we isolated h ...201627552108
cotton leaf curl multan virus-derived viral small rnas can target cotton genes to promote viral infection.rna silencing is a conserved mechanism in plants that targets viruses. viral small rnas (vsirnas) can be generated from viral double-stranded rna replicative intermediates within the infected host, or from host rna-dependent rna polymerases activity on viral templates. the abundance and profile of vsirnas in viral infections have been reported previously. however, the involvement of vsirnas during infection of the geminiviridae family member cotton leaf curl virus (clcud), which causes significa ...201627540385
mapping qtls for drought tolerance in an f2:3 population from an inter-specific cross between gossypium tomentosum and gossypium hirsutum.cotton is one of the most important natural fiber crops in the world. its growth and yield is greatly limited by drought. a quantitative trait locus (qtl) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (gossypium spp) using 188 f2:3 lines developed from an inter-specific cross between a wild cotton species, g. tomentosum, and an upland cotton, g. hirsutum (cri-12). a genetic map was constructed using 1295 simple sequence repeat markers, which amp ...201627525919
electron microscopic cytochemistry of cyclopropenoids in cottonseed (gossypium hirsutum l.) tissues.an electron microscopic cytochemical procedure for locating cyclopropenoids (cyp) in oilseeds is proposed. the procedure is based on the fact that metallic silver is produced when agno3 reacts with cyp. since so many other compounds react with agno3, we treated specimens that do and not contain cyp with agno3 and looked for electron-dense stains that were unique to cyp(+) seeds. virtually everything in all seed specimens, regardless of the presence or absence of cyp, was stained. however, cotton ...198727519735
de novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (g. hirsutum l.).plant regeneration via somatic embryogenesis (se) is the key step for genetic improvement of cotton (gossypium hirsutum l.) through genetic engineering mediated by agrobacteria, but the molecular mechanisms underlying se in cotton is still unclear. here, rna-sequencing was used to analyze the genes expressed during se and their expression dynamics using rnas isolated from non-embryogenic callus (nec), embryogenic callus (ec) and somatic embryos (ses). a total of 101, 670 unigenes were de novo as ...201627511192
fine mapping and identification of candidate genes for a qtl affecting meloidogyne incognita reproduction in upland cotton.the southern root-knot nematode (meloidogyne incognita; rkn) is one of the most important economic pests of upland cotton (gossypium hirsutum l.). host plant resistance, the ability of a plant to suppress nematode reproduction, is the most economical, practical, and environmentally sound method to provide protection against this subterranean pest. the resistant line auburn 623rnr and a number of elite breeding lines derived from it remain the most important source of root-knot nematode (rkn) res ...201627503539
the fungal endophyte chaetomium globosum negatively affects both above- and belowground herbivores in cotton.mutualistic plant-endophyte symbioses can benefit plants by increasing host fitness through reductions in herbivory. the fungus, chaetomium globosum strain tamu 520, was previously isolated as an endophyte from cotton (gossypium hirsutum) and can be re-inoculated to systemically colonize cotton plants via seed treatment. we evaluated the potential impacts of the endophyte in cotton on plant parasitic nematodes belowground, along with piercing-sucking and chewing insects aboveground. endophytic c ...201627451418
response and tolerance mechanism of cotton gossypium hirsutum l. to elevated temperature stress: a review.cotton is an important multipurpose crop which is highly sensitive to both biotic and abiotic stresses. proper management of this cash crop requires systematic understanding of various environmental conditions that are vital to yield and quality. high temperature stress can severely affect the viability of pollens and anther indehiscence, which leads to significant yield losses. cotton can respond to withstand adverse environmental condition in several phases among which the accumulation of chem ...201627446165
effect of waterlogging on carbohydrate metabolism and the quality of fiber in cotton (gossypium hirsutum l.).transient waterlogging occurs frequently in the yangtze river and adversely affects cotton fiber quality. however, the carbohydrate metabolic mechanism that affects fiber quality after waterlogging remains undescribed. here, the effects of five waterlogging levels (0, 3, 6, 9, and 12 days) were assessed during flowering and boll formation to characterize the carbohydrates, enzymes and genes that affect the fiber quality of cotton after waterlogging. the cellulose and sucrose contents of cotton f ...201627446110
stability of spatial distributions of stink bugs, boll injury, and ndvi in cotton.a 3-yr study was conducted to determine the degree of aggregation of stink bugs and boll injury in cotton, gossypium hirsutum l., and their spatial association with a multispectral vegetation index (normalized difference vegetation index [ndvi]). using the spatial analysis by distance indices analyses, stink bugs were less frequently aggregated (17% for adults and 4% for nymphs) than boll injury (36%). ndvi values were also significantly aggregated within fields in 19 of 48 analyses (40%), with ...201627436843
ghataf1, a nac transcription factor, confers abiotic and biotic stress responses by regulating phytohormonal signaling networks.dual function of ghataf1 in the responses to salinity stress and verticillium dahliae infection in cotton. nac (nam/ataf1/2/cuc2) is a large plant-specific transcription factor family that plays important roles in the response to abiotic stresses. we previously isolated a cotton nac transcription factor gene, ghataf1, which was up-regulated by aba, cold and salt stresses and classified into afat1/2, a sub-family of nac. here, we report that ghataf1 was also highly induced by meja, sa and vertici ...201627432176
overexpression of ghsarp1 encoding a e3 ligase from cotton reduce the tolerance to salt in transgenic arabidopsis.ubiquitination plays a very important role in the response to abiotic stresses of plant. to identify key regulators of salt stress, a gene ghsarp1(salt-associated ring finger protein)encoding c3h2c3-type e3 ligase, was cloned from cotton. transcription level of ghsarp1 was high in leaf, flower and fiber of 24,27 and 27dpa (days post-anthesis), but low in root and stem. except peg6000 treatment, the expression of ghsarp1 was down-regulated by nacl, cold and aba after being treated for 1 h. ghsarp ...201627402266
high expression of cry1ac protein in cotton (gossypium hirsutum) by combining independent transgenic events that target the protein to cytoplasm and plastids.transgenic cotton was developed using two constructs containing a truncated and codon-modified cry1ac gene (1,848 bp), which was originally characterized from bacillus thuringiensis subspecies kurstaki strain hd73 that encodes a toxin highly effective against many lepidopteran pests. in construct i, the cry1ac gene was cloned under fmvde, a strong constitutively expressing promoter, to express the encoded protein in the cytoplasm. in construct ii, the encoded protein was directed to the plastids ...201627391960
evaluation of brevibacillus brevis as a potential plant growth promoting rhizobacteria for cotton (gossypium hirsutum) crop.the present investigation was undertaken to isolate, screen and evaluate a selected promising pgpr brevibacillus brevis on cotton crop. out of 156 bacterial isolates one of the most promising isolate was analyzed for the various pgp traits. a seed germination analysis was conducted with cotton seeds to evaluate the potential of the isolate to promote plant growth. the bacterial isolate was checked for its growth and survival at high temperatures. the isolate was also analyzed for the pgp traits ...201627386392
structure of exogenous gene integration and event-specific detection in the glyphosate-tolerant transgenic cotton line bg2-7.in this study, the flanking sequence of an inserted fragment conferring glyphosate tolerance on transgenic cotton line bg2-7 was analyzed by thermal asymmetric interlaced polymerase chain reaction (tail-pcr) and standard pcr. the results showed apparent insertion of the exogenous gene into chromosome d10 of the gossypium hirsutum l. genome, as the left and right borders of the inserted fragment are nucleotides 61,962,952 and 61,962,921 of chromosome d10, respectively. in addition, a 31-bp cotton ...201627379683
partial life history of chrysodeixis includens (lepidoptera: noctuidae) on summer hosts.the soybean looper, chrysodeixis includens (walker) (lepidoptera: noctuidae), is a major defoliating pest of soybeans, glycine max (l.) merrill, in louisiana. however, other alternate host crops in the agroecosystem have the potential to impact c. includens populations. life table statistics of c. includens on four host plants were evaluated. c. includens larvae were fed leaves of three cotton gossypium hirsutum l. cultivars 'dp 143 b2rf,' 'dp 174 rf,' and 'phy 485 wrf'; cowpea vigna unguiculata ...201627375294
mining and analysis of snp in response to salinity stress in upland cotton (gossypium hirsutum l.).salinity stress is a major abiotic factor that affects crop output, and as a pioneer crop in saline and alkaline land, salt tolerance study of cotton is particularly important. in our experiment, four salt-tolerance varieties with different salt tolerance indexes including cri35 (65.04%), kanghuanwei164 (56.19%), zhong9807 (55.20%) and cri44 (50.50%), as well as four salt-sensitive cotton varieties including hengmian3 (48.21%), gk50 (40.20%), xinyan96-48 (34.90%), zhongs9612 (24.80%) were used a ...201627355327
genetic effects and heterosis of yield and yield component traits based on gossypium barbadense chromosome segment substitution lines in two gossypium hirsutum backgrounds.we hybridized 10 chromosome segment substitution lines (cssls) each from two cssl populations and produced 50 f1 hybrids according to north carolina design ii. we analyzed the genetic effects and heterosis of yield and yield components in the f1 hybrids and parents in four environments via the additive-dominance genetic model. yield and yield components of the cssls were controlled by combined additive and dominance effects, and lint percentage was mainly controlled by additive effects, but boll ...201627348815
the cotton mitogen-activated protein kinase kinase 3 functions in drought tolerance by regulating stomatal responses and root growth.mitogen-activated protein kinase (mapk) cascades play critical roles in signal transduction processes in eukaryotes. the mapk kinases (mapkks) that link mapkk kinases (mapkkks) and mapks are key components of mapk cascades. however, the intricate regulatory mechanisms that control mapkks under drought stress conditions are not fully understood, especially in cotton (gossypium hirsutum) here, we isolated and characterized the cotton group b mapkk gene ghmkk3 overexpressing ghmkk3 in nicotiana ben ...201627335349
optimal cotton insecticide application termination timing: a meta-analysis.the concept of insecticide termination timing is generally accepted among cotton (gossypium hirsutum) researchers; however, exact timings are often disputed. specifically, there is uncertainty regarding the last economic insecticide application to control fruit-feeding pests including tarnished plant bug (lygus lineolaris (palisot de beauvois)), boll weevil (anthonomus grandis), bollworm (helicoverpa zea), tobacco budworm (heliothis virescens), and cotton fleahopper (pseudatomoscelis seriatus). ...201627329633
ssr-based association mapping of salt tolerance in cotton (gossypium hirsutum l.).the identification of simple sequence repeat (ssr) markers associated with salt tolerance in cotton contributes to molecular assisted selection (mas), which can improve the efficiency of traditional breeding. in this study, 134 samples of upland cotton cultivars were selected. the seedling emergence rates were tested under 0.3% nacl stress. a total of 74 ssr markers were used to scan the genomes of these samples. to identify ssr markers associated with salt tolerance, an association analysis was ...201627323090
trichomes control flower bud shape by linking together young petals.trichomes are widespread in plants and develop from surface cells on different tissues(1). they have many forms and functions, from defensive spines to physical barriers that trap layers of air to insulate against desiccation, but there is growing evidence that trichomes can also have developmental roles in regulating flower structure(2,3). we report here that the trichomes on petals of cotton, gossypium hirsutum l., are essential for correct flower bud shape through a mechanical entanglement of ...201627322517
cotton cytosolic pyruvate kinase ghpk6 participates in fast fiber elongation regulation in a ros-mediated manner.cotton cytosolic pyruvate kinase ghpk6 is preferentially expressed in the late stage of fiber elongation process, transgenic experiments indicated that its expression level was negatively correlated to cell expansion rate. pyruvate kinase (pk) plays vital regulatory roles in rapid cell growth in mammals. however, the function of pk in plant cell growth remains unclear. in allotetraploid upland cotton (gossypium hirsutum l.), a total of 33 pk genes are encoded by the genome. analysis of the trans ...201627316434
coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum).genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ...201627314265
inoculant of arbuscular mycorrhizal fungi (rhizophagus clarus) increase yield of soybean and cotton under field conditions.nutrient availability is an important factor in crop production, and regular addition of chemical fertilizers is the most common practice to improve yield in agrosystems for intensive crop production. the use of some groups of microorganisms that have specific activity providing nutrients to plants is a good alternative, and arbuscular mycorrhizal fungi (amf) enhance plant nutrition by providing especially phosphorus, improving plant growth and increasing crop production. unfortunately, the use ...201627303367
leaf ontogeny strongly influences photosynthetic tolerance to drought and high temperature in gossypium hirsutum.temperature and drought are major abiotic limitations to crop productivity worldwide. while abiotic stress physiology research has focused primarily on fully expanded leaves, no studies have investigated photosynthetic tolerance to concurrent drought and high temperature during leaf ontogeny. to address this, gossypium hirsutum plants were exposed to five irrigation treatments, and two different leaf stages were sampled on three dates during an abnormally dry summer. early in the growing season, ...201627302003
cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis.cotton fibers arise through highly anisotropic expansion of a single seed epidermal cell. we obtained evidence that apical cell wall synthesis occurs through examining the tips of young elongating gossypium hirsutum (gh) and g. barbadense (gb) fibers. we characterized two tip types in gh fiber (hemisphere and tapered), each with distinct apical diameter, central vacuole location, and distribution of cell wall components. the apex of gh hemisphere tips was enriched in homogalacturonan epitopes, i ...201627301434
assessment of bt trait purity in different generations of transgenic cottons.adequate expression of bt (bacillus thuringiensis) toxins and purity of seeds of bt-transgenic cottons are important for controlling bollworms, and thereby increasing the cotton productivity. therefore, we examined the variability in expression of bt toxin proteins in the seeds and in leaves of different cotton (gossypium hirsutum (l.) hybrids (jkch 226, jkch 1947, jkch durga, jkch ishwar, jkch varun kdchh 441 and kdchh 621) expressing bt toxins in f₁ and f₂ generations, using bioassays against ...201627295920
genome-wide analysis of long noncoding rnas and their responses to drought stress in cotton (gossypium hirsutum l.).recent researches on long noncoding rnas (lncrnas) have expanded our horizon of gene regulation and the cellular complexity. however, the number, characteristics and expression patterns of lncrnas remain poorly characterized and how these lncrnas biogenesis are regulated in response to drought stress in cotton are still largely unclear. in the study, using a reproducibility-based rna-sequencing and bioinformatics strategy to analyze the lncrnas of 9 samples under three different environment stre ...201627294517
monopodial and sympodial branching architecture in cotton is differentially regulated by the gossypium hirsutum single flower truss and self-pruning orthologs.domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a day-neutral annual row-crop. residual perennial traits, however, complicate irrigation and crop management, and more determinate architectures are desired. cotton simultaneously maintains robust monopodial indeterminate shoots and sympodial determinate shoots. we questioned if and how the flowering locus t/single flower truss (sft)-like and terminal flower1/self-pruning (sp)-like genes cont ...201627292411
gherf-iib3 regulates the accumulation of jasmonate and leads to enhanced cotton resistance to blight disease.the phytohormone jasmonic acid (ja) and its derivatives, collectively referred to as jasmonates, regulate many developmental processes, but are also involved in the response to numerous abiotic/biotic stresses. thus far, powerful reverse genetic strategies employing perception, signalling or biosynthesis mutants have broadly contributed to our understanding of the role of ja in the plant stress response and development, as has the chemical gain-of-function approach based on exogenous application ...201627291786
independent domestication of two old world cotton species.domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ...201627289095
effects of silicon treatment and inoculation with fusarium oxysporum f. sp. vasinfectum on cellular defences in root tissues of two cotton cultivars.silicon has been shown to enhance the resistance of plants to fungal and bacterial pathogens. here, the effect of potassium silicate was assessed on two cotton (gossypium hirsutum) cultivars subsequently inoculated with fusarium oxysporum f. sp. vasinfectum (fov). sicot 189 is moderately resistant whilst sicot f-1 is the second most resistant commercial cultivar presently available in australia.201627288509
evolution of gene duplication in plants.ancient duplication events and a high rate of retention of extant pairs of duplicate genes have contributed to an abundance of duplicate genes in plant genomes. these duplicates have contributed to the evolution of novel functions, such as the production of floral structures, induction of disease resistance, and adaptation to stress. additionally, recent whole-genome duplications that have occurred in the lineages of several domesticated crop species, including wheat (triticum aestivum), cotton ...201627288366
cotton (gossypium hirsutum l.) boll rotting bacteria vectored by the brown stink bug, euschistus servus (say) (hemiptera: pentatomidae).to determine the capacity of the brown stink bug (bsb) (euschistus servus) to transmit an infective pantoea agglomerans strain sc 1-r into cotton (gossypium hirsutum) bolls.201627261673
a comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a gossypium hirsutum × gossypium barbadense population.upland cotton (gossypium hirsutum l.) is the most important fiber crop, and its lint-yield improvement is impeded due to its narrow genetic base and the lack of understanding of the genetic basis of yield. backcross inbred lines (bils) or near-isogenic lines (nils) in the same genetic background differing in lint yield, developed through advanced backcrossing, provide an important genomic resource to study the molecular genetic basis of lint yield. in the present study, a high-yield (hy) group a ...201627256327
a cotton annexin affects fiber elongation and secondary cell wall biosynthesis associated with ca2+ influx, ros homeostasis, and actin filament reorganization.annexins play pivotal roles in a variety of cellular processes as well as in fiber development; however, the functional mechanisms of their activities are unclear. here, an annexin gene that is preferentially expressed in fibers, ghfannxa, was found to be significantly associated with various cotton (gossypium hirsutum) fiber traits. transgenic analysis demonstrated that ghfannxa affected cotton fiber elongation and was involved in secondary cell wall (scw) biosynthesis. functional studies demon ...201627255486
asiatic cotton can generate similar economic benefits to bt cotton under rainfed conditions.american cotton (gossypium hirsutum l.), transformed with bacillus thuringiensis cry genes (bt g. hirsutum) that confer resistance to lepidopteran pests, is extensively cultivated worldwide. in india, transgenic bt g. hirsutum was commercially released in 2002 and by 2014 95% of farmers had adopted bt g. hirsutum(1). the economic benefits of bt g. hirsutum over non-bt g. hirsutum are well documented and include increase in yields, increase in farmers' net revenue and reduction in pesticide appli ...201527250007
overexpression of cotton ghmpk11 decreases disease resistance through the gibberellin signaling pathway in transgenic nicotiana benthamiana.many changes in development, growth, hormone activity and environmental stimuli responses are mediated by mitogen-activated protein kinase (mapk) cascades. however, in plants, studies on mapks have mainly focused on mpk3, mpk4 and mpk6. here, a novel group b mapk gene, ghmpk11, was isolated from cotton (gossypium hirsutum l.) and characterized. both promoter and expression pattern analyses revealed that ghmpk11 is involved in defense responses and signaling pathways. ghmpk11 overexpression in ni ...201627242882
effects of soil salinity on sucrose metabolism in cotton leaves.this study investigated sucrose metabolism of the youngest fully expanded main-stem leaf (msl) and the subtending leaf of cotton (gossypium hirsutum l.) boll (lscb) of salt-tolerant (ccri-79) and salt-sensitive (simian 3) cultivars and its relationship to boll weight under low, medium and high soil salinity stress in dafeng, china, in 2013 and 2014. the results showed that with increased soil salinity, 1) both the chlorophyll content and net photosynthetic rate (pn) decreased, while the internal ...201627228029
genome-wide comparative analysis of the phospholipase d gene families among allotetraploid cotton and its diploid progenitors.in this study, 40 phospholipase d (pld) genes were identified from allotetraploid cotton gossypium hirsutum, and 20 pld genes were examined in diploid cotton gossypium raimondii. combining with 19 previously identified gossypium arboreum pld genes, a comparative analysis was performed among the pld gene families among allotetraploid and two diploid cottons. based on the orthologous relationships, we found that almost each g. hirsutum pld had a corresponding homolog in the g. arboreum and g. raim ...201627213891
performance of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae) under variable conditions of temperature and mating.mealybugs have strong associations with their host plants due to their limitations for dispersal. thus, environmental conditions and host quality may impact the biological traits of mealybugs. to the best of our knowledge, we are the first to report on the biology of a brazilian population of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae), which has recently been reported to infest cotton in brazil. we evaluated the development and reproductive performance of f. virg ...201427193398
development and validation of a fluorescent multiplexed immunoassay for measurement of transgenic proteins in cotton (gossypium hirsutum).in order to provide farmers with better and more customized alternatives to improve yields, combining multiple genetically modified (gm) traits into a single product (called stacked trait crops) is becoming prevalent. trait protein expression levels are used to characterize new gm products and establish exposure limits, two important components of safety assessment. developing a multiplexed immunoassay capable of measuring all trait proteins in the same sample allows for higher sample throughput ...201627177195
a genetic map between gossypium hirsutum and the brazilian endemic g. mustelinum and its application to qtl mapping.among the seven tetraploid cotton species, little is known about transmission genetics and genome organization in gossypium mustelinum, the species most distant from the source of most cultivated cotton, g. hirsutum in this research, an f2 population was developed from an interspecific cross between g. hirsutum and g. mustelinum (hm). a genetic linkage map was constructed mainly using simple sequence repeat (ssrs) and restriction fragment length polymorphism (rflp) dna markers. the arrangements ...201627172208
the immature fiber mutant phenotype of cotton (gossypium hirsutum) is linked to a 22-bp frame-shift deletion in a mitochondria targeted pentatricopeptide repeat gene.cotton seed trichomes are the most important source of natural fibers globally. the major fiber thickness properties influence the price of the raw material, and the quality of the finished product. the recessive immature fiber (im) gene reduces the degree of fiber cell wall thickening by a process that was previously shown to involve mitochondrial function in allotetraploid gossypium hirsutum here, we present the fine genetic mapping of the im locus, gene expression analysis of annotated protei ...201627172184
genome wide ssr high density genetic map construction from an interspecific cross of gossypium hirsutum × gossypium tomentosum.a high density genetic map was constructed using f2 population derived from an interspecific cross of g. hirsutum × g. tomentosum. the map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cm of cotton genome with an average inter-marker distance of 1.48 cm. the maximum length of chromosome was 218.38 cm and the minimum was 122.09 cm with an average length of 167.90 cm. a sub-genome covers more genetic distance (2189.01 cm) with an average inter loci dist ...201627148280
epistasis together with partial dominance, over-dominance and qtl by environment interactions contribute to yield heterosis in upland cotton.qtl mapping based on backcross and ril populations suggests that epistasis together with partial dominance, over-dominance and their environmental interactions of qtls play an important role in yield heterosis in upland cotton. a backcross population (bc) was constructed to explore the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). for yield and yield components, recombinant inbred line (ril) and bc populations were evaluated simultaneously at three different locations. a t ...201627138784
chlorophyll fluorescence induction kinetics and yield responses in rainfed crops with variable potassium nutrition in k deficient semi-arid alfisols.optimum potassium (k) nutrition in semi-arid regions may help crop plants to overcome constraints in their growth and development such as moisture stress, leading to higher productivity of rainfed crops, thus judicious k management is essential. a study was conducted to evaluate the importance of k nutrition on physiological processes like photosynthesis through chlorophyll a fluorescence and chlorophyll fluorescence induction kinetics (ojip) of rainfed crops viz., maize (zea mays l.), pearl mil ...201627101276
fine mapping and rna-seq unravels candidate genes for a major qtl controlling multiple fiber quality traits at the t1 region in upland cotton.improving fiber quality is a major challenge in cotton breeding, since the molecular basis of fiber quality traits is poorly understood. fine mapping and candidate gene prediction of quantitative trait loci (qtl) controlling cotton fiber quality traits can help to elucidate the molecular basis of fiber quality. in our previous studies, one major qtl controlling multiple fiber quality traits was identified near the t1 locus on chromosome 6 in upland cotton.201627094760
fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (gossypium hirsutum).high-quality cotton fibre equates to a more comfortable textile. fibre length is an important index of fibre quality. hydrogen peroxide (h2o2) acts as a signalling molecule in the regulation of fibre elongation. results from in vitro ovule culture suggest that the alteration of fibre cell h2o2 levels affects fibre development. ascorbate peroxidase (apx) is an important reactive oxygen species (ros) scavenging enzyme, and we found that ghapx1at/dt encoded one member of the previously unrealized g ...201627091877
development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene.cotton leaf curl virus (clcuv) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. the transgenic cotton (gossypium hirsutum cv. coker 310) plants were developed by using βc1 gene in antisense orientation gene driven by cauliflower mosaic virus-35s promoter and nos (nopaline synthase) terminator and mediated by agrobacterium tumefaciens transformation and somatic embryogenesis system. molecular confirmation of the transformants was carried ...201627081361
functional characterization of a basic helix-loop-helix (bhlh) transcription factor ghdel65 from cotton (gossypium hirsutum).cotton fiber is proposed to share some similarity with the arabidopsis thaliana leaf trichome, which is regulated by the myb-bhlh-wd40 transcription complex. although several myb transcription factors and wd40 family proteins in cotton have been characterized, little is known about the role of bhlh family proteins in cotton. here, we report that ghdel65, a bhlh protein from cotton (gossypium hirsutum), is a functional homologue of arabidopsis glabra3 (gl3) and enhancer of glabra3 (egl3) in regul ...201627080593
genomic heterozygosity and hybrid breakdown in cotton (gossypium): different traits, different effects.hybrid breakdown has been well documented in various species. relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. but correlations between genomic heterozygosity and vegetative and reproductive traits in cotton interspecific populations have not been studied. in the current study, two reciprocal f2 populations were developed using gossypium hirsutum cv. emian 22 and g. barbadense acc. 3-79 as parents to study hybri ...201627072350
construction of a high-density genetic map by specific locus amplified fragment sequencing (slaf-seq) and its application to quantitative trait loci (qtl) analysis for boll weight in upland cotton (gossypium hirsutum.).upland cotton (gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for the industrial production and everyday use. next-generation sequencing is a powerful method to identify single nucleotide polymorphism markers on a large scale for the construction of a high-density genetic map for quantitative trait loci mapping.201627067834
comparative transmission genetics of introgressed chromatin in gossypium (cotton) polyploids.introgression is widely acknowledged as a potential source of valuable genetic variation, and growing effort is being invested in analysis of interspecific crosses conferring transgressive variation. experimental backcross populations provide an opportunity to study transmission genetics following interspecific hybridization, identifying opportunities and constraints to introgressive crop improvement. the evolutionary consequences of introgression have been addressed at the theoretical level, ho ...201627056931
generation of transcriptome profiling and gene functional analysis in gossypium hirsutum upon verticillium dahliae infection.verticillium wilt caused by the soil-borne fungus verticillium dahliae, is a devastating disease in cotton. to get more candidate genes related to wilt resistance, a normalized, full-length cdna library was used to generate the transcriptome profile from a wilt-resistant gossypium hirsutum variety (zhongzhimian kv3) upon v. dahliae strain v991 infection. total 3486 high-quality ests were focused from randomly selected 4000 clones, which included 3065 singletons and 421 contigs. to categorize the ...201627038549
effects of single and dual species herbivory on the behavioural responses of three thrips species to cotton seedlings.this study investigated the olfactory responses of three thrips species [frankliniella schultzei trybom, f. occidentalis pergrande and thrips tabaci lindeman (thysanoptera: thripidae)] to cotton seedlings [gossypium hirsutum l. (malvales: malvaceae)] simultaneously damaged by different combinations of herbivores. cotton seedlings were damaged by foliar feeding tetranychus urticae koch (trombidiforms: tetranychidae), helicoverpa armigera hübner (lepidoptera: noctuidae), aphis gossypii glover (hem ...201627029603
rnai-mediated resistance against cotton leaf curl disease in elite indian cotton (gossypium hirsutum) cultivar narasimha.cotton leaf curl disease (clcud) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. clcud is a serious threat for the cultivation of cotton (gossypium sp.) and several species in the family malvaceae. in this study, rnai-based approach was applied to generate transgenic cotton (gossypium hirsutum) plants resistant to cotton leaf curl rajasthan virus (clcurv). an intron hairpin (ihp) rnai construct capa ...201627028623
insights into the ecology and evolution of polyploid plants through network analysis.polyploidy is a widespread phenomenon throughout eukaryotes, with important ecological and evolutionary consequences. although genes operate as components of complex pathways and networks, polyploid changes in genes and gene expression have typically been evaluated as either individual genes or as a part of broad-scale analyses. network analysis has been fruitful in associating genomic and other 'omic'-based changes with phenotype for many systems. in polyploid species, network analysis has the ...201627027619
mapping of genes for flower-related traits and qtls for flowering time in an interspecific population of gossypium hirsutum × g. darwinii. 201627019451
genome-wide identification and analysis of the stress-resistance function of the tps (trehalose-6-phosphate synthase) gene family in cotton.trehalose (a-d-glucopyranosyl a-d-glucopyranoside) is a nonreducing disaccharide and is widely distributed in bacteria, fungi, algae, plants and invertebrates. in the study, the identification of trehalose-6-phosphate synthase (tps) genes stress-related in cotton, and the genetic structure analysis and molecular evolution analysis of tpss were conducted with bioinformatics methods, which could lay a foundation for further research of tps functions in cotton.201626993467
to be a flower or fruiting branch: insights revealed by mrna and small rna transcriptomes from different cotton developmental stages.the architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. nulliplex branch is a useful phenotype to study cotton architecture. we used rna sequencing to obtain mrna and mirna profiles from nulliplex- and normal-branch cotton at three developmental stages. the differentially expressed genes (degs) and mirnas were identified that preferentially/specifica ...201626983497
simultaneous overexpression of the hherf2 and pedreb2a genes enhanced tolerances to salt and drought in transgenic cotton.a desert-grown halimodendron halodendron ethylene-responsive element binding factor gene (hherf2), which encodes a 245 amino acids protein containing a conserved ap2/erebp domain, was isolated through the rapid amplification cdna end (race) method. sequence and phylogenetic analysis indicated that hherf2 was classified into the b-2 group of erf subfamily. semiquantitative rt-pcr showed that hherf2 was greatly induced by treatments with high-salt, drought and low temperature in h. halodendron see ...201626972971
critical roles of vacuolar invertase in floral organ development and male and female fertilities are revealed through characterization of ghvin1-rnai cotton plants.seed number and quality are key traits determining plant fitness and crop yield and rely on combined competence in male and female fertilities. sucrose metabolism is central to reproductive success. it remains elusive, though, how individual sucrose metabolic enzymes may regulate the complex reproductive processes. here, by silencing vacuolar invertase (vin) genes in cotton (gossypium hirsutum) reproductive organs, we revealed diverse roles that vin plays in multiple reproductive processes. a se ...201626969720
structural analysis of gossypium hirsutum fibers grown under greenhouse and hydroponic conditions.cotton is the one of the world's most important crops. like any other crop, cotton growth/development and fiber quality is highly dependent on environmental factors. increasing global weather instability has been negatively impacting its economy. cotton is a crop that exerts an intensive pressure over natural resources (land and water) and demands an overuse of pesticides. thus, the search for alternative cotton culture methods that are pesticide-free (biocotton) and enable customized standard f ...201626965558
data for proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.).cotton is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to study the male sterile mechanisms of ccri9106, histological and itraq-facilitated proteomic analyses of anthers were performed. this data article contains data related to the research article titled itraq-facilitated proteomic profiling of ...201526958592
identification of stable quantitative trait loci (qtls) for fiber quality traits across multiple environments in gossypium hirsutum recombinant inbred line population.the identification of quantitative trait loci (qtls) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (mas). in the present study, we used 28,861 simple sequence repeat (ssr) markers, which included 12,560 gossypium raimondii (d genome) sequence-based ssr markers to identify polymorphism between two upland cotton strains 0-153 and sgk9708. a total of 851 polymorphic primers were finally selected and used to genotype ...201626951621
anthocyanin accumulation enhanced in lc-transgenic cotton under light and increased resistance to bollworm.breeding of naturally colored cotton fiber has been hampered by the limited germplasm, an alternative way is to use transgenic approach to create more germplasm for breeding. here, we report our effort to engineer anthocyanin production in cotton. the maize lc gene, under the control of the constitutive 35s promoter, was introduced into cotton through genetic transformation. our data showed that the expression of the lc gene alone is sufficient to trigger the accumulation of anthocyanin in a var ...201726941851
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress.drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. to determine the effects of drought stress on cotton seedlings, we used tw ...201626941218
transgenic cotton plants expressing cry1ia12 toxin confer resistance to fall armyworm (spodoptera frugiperda) and cotton boll weevil (anthonomus grandis).gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including cry toxins, proteinase inhibitors, and toxic peptides, among others. in the present study, we developed transgenic cotton plants by fertilized floral buds injection (through ...201626925081
antisense expression of gossypium hirsutum udp-glucuronate decarboxylase in arabidopsis leads to changes in cell wall components.udp-glucuronate decarboxylase (udp-xylose synthase; uxs, ec 4.1.1.35) is an essential enzyme of the non-cellulosic polysaccharide biosynthetic pathway. in the present study, using transient expression of fluorescently labeled gossypium hirsutum uxs (ghuxs3) protein in onion epidermal cells, we observed that this protein was distributed in the cytoplasm. the ghuxs3 cdna of cotton was expressed in an antisense orientation in arabidopsis thaliana by agrobacterium tumefaciens-mediated transformation ...201626909959
global analysis of the developmental dynamics of gossypium hirsutum based on strand-specific transcriptome.cotton is an economically important crop that provides both natural fiber and by-products such as oil and protein. its global gene expression could provide insight into the biological processes underlying growth and development, which involve suites of genes expressed with temporal and spatial control by regulatory networks. generally, the goal for cotton breeding is improvement of the fiber; thus, most previous research has focused on identifying genes specific to the fiber. however, seeds may ...201626892265
diversity in betasatellites associated with cotton leaf curl disease during source-to-sink movement through a resistant host.cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. the disease symptoms are induced by dna satellite known as cotton leaf curl multan betasatellite (clcumub), dominant betasatellite in cotton but another betasatellite known as chili leaf curl betasatellite (chlcb) is also found associated with the disease. grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cot ...201626889114
mapping by sequencing in cotton (gossypium hirsutum) line md52ne identified candidate genes for fiber strength and its related quality attributes.three qtl regions controlling three fiber quality traits were validated and further fine-mapped with 27 new single nucleotide polymorphism (snp) markers. transcriptome analysis suggests that receptor-like kinases found within the validated qtls are potential candidate genes responsible for superior fiber strength in cotton line md52ne. fiber strength, length, maturity and fineness determine the market value of cotton fibers and the quality of spun yarn. cotton fiber strength has been recognized ...201626883043
relationship between potassium fertilization and nitrogen metabolism in the leaf subtending the cotton (gossypium hirsutum l.) boll during the boll development stage.the nitrogen (n) metabolism of the leaf subtending the cotton boll (lscb) was studied with two cotton (gossypium hirsutum l.) cultivars (simian 3, low-k tolerant; siza 3, low-k sensitive) under three levels of potassium (k) fertilization (k0: 0 g k2o plant(-1), k1: 4.5 k2o plant(-1) and k2: 9.0 g k2o plant(-1)). the results showed that total dry matter increased by 13.1-27.4% and 11.2-18.5% under k supply for simian 3 and siza 3. boll biomass and boll weight also increased significantly in k1 an ...201626874296
the cotton myb108 forms a positive feedback regulation loop with cml11 and participates in the defense response against verticillium dahliae infection.accumulating evidence indicates that plant myb transcription factors participate in defense against pathogen attack, but their regulatory targets and related signaling processes remain largely unknown. here, we identified a defense-related myb gene (ghmyb108) from upland cotton (gossypium hirsutum) and characterized its functional mechanism. expression of ghmyb108 in cotton plants was induced by verticillium dahliae infection and responded to the application of defense signaling molecules, inclu ...201626873979
h2o2 and aba signaling are responsible for the increased na+ efflux and water uptake in gossypium hirsutum l. roots in the non-saline side under non-uniform root zone salinity.non-uniform root salinity increases the na(+)efflux, water use, and growth of the root in non-saline side, which may be regulated by some form of signaling induced by the high-salinity side. however, the signaling and its specific function have remained unknown. using a split-root system to simulate a non-uniform root zone salinity in gossypium hirsutum l., we showed that the up-regulated expression of sodium efflux-related genes (sos1, sos2, pma1, and pma2) and water uptake-related genes (pip1 ...201626862153
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