Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| adhesion of periodontal bacteria to titanium, and titanium alloy powders. | in this study, the adhesion of radioactively labeled actinomyces viscosus (a. viscosus), actinobacillus actinomicetemcomitans (aa) and porphyromonas gingivalis (p. gingivalis) to titanium (ti) and ti-6-al-4v alloy (ti-alloy) coated with albumin or human saliva was investigated. all the tested bacteria displayed greater attachment to ti-alloy than to ti. p. gingivalis exhibited less adhesion to ti and ti-alloy than did the other bacterial strains. adhesion of a. viscosus and aa was greatly reduce ... | 1998 | 9663033 |
| the efficacy of antimicrobial mouth rinses in oral health care. | there is growing public recognition of the importance of oral health, as symbolized by the theme. "oral health for a healthy life" proposed for the 1994 world health day. in this report, the efficacy of antimicrobial mouth rinses, mainly listerine, was reviewed by three investigators who are working as a microbiologist, a microbiologist, a dentist, and a dental hygienist participating in oral health care. listerine, an antimicrobial mouth rinse, completely killed microorganisms in 10 to 30 secon ... | 1998 | 9663026 |
| binding properties of streptococcal glucosyltransferases for hydroxyapatite, saliva-coated hydroxyapatite, and bacterial surfaces. | the binding specificities of streptococcus glucosyltransferase (gtf) b, c and d for hydroxyapatite (ha), saliva-coated hydroxyapatite (sha), and bacterial surfaces were examined. for ha beads the following values were obtained: (k = affinity; n = number of binding sites) gtfb, k = 46 x 10(5) ml/mumol, n = 0.65 x 10(-6) mumol/m2; gtfc, k = 86 x 10(5) ml/mumol, n = 4.42 x 10(-6) mumol/m2.; gtfd, k = 100 x 10(5) ml/mumol, n = 0.83 x 10(-6) mumol/m2. for sha beads, the following values were obtained ... | 1998 | 9602288 |
| antibacterial activity of dentine primer containing mdpb after curing. | a monomer methacryloyloxydodecylpyridinium bromide (mdpb) has antibacterial activity before polymerization. furthermore, the antibacterial agent is immobilized by the polymerization of mdpb and the resin-based material incorporating mdpb is able to show an antibacterial effect even after being cured. the purpose of this study was to investigate the antibacterial effect of a dentine primer containing mdpb after curing. | 1998 | 9594480 |
| the influence of dietary sugars and starch on the establishment of streptococcus mutans and actinomyces viscosus in dental plaque of specific pathogen-free rats. | the establishment of s. mutans together with a. viscosus was investigated in dental plaque of specific pathogen-free (spf) rats fed different carbohydrate diets. two tanzanian s.mutans strains mm3 and mm24 and one tanzanian a. viscosus strain mm13 were used for this purpose. the basic diet consisting of 32% skim-milk, 7% yeast extract and 1% soy bean oil was supplemented with either 10% lactose and 50% corn flour, or 10% glucose and 50% corn flour, or 60% amylum or 60% wheat flour. s. mutans and ... | 1993 | 9590873 |
| characteristics of systemic antibody responses of nonhuman primates to cell envelope and cell wall antigens from periodontal pathogens. | the immune response of the primate, macaca fascicularis, to cell envelope (cea) or cell wall (cwa) antigens of several periodontal pathogens was examined to develop a strategy to interfere with ligature-induced periodontitis. animals were parenterally immunized with cea of either porphyromonas gingivalis, prevotella intermedia or a combination of cea/cwa of campylobacter rectus, fusobacterium nucleatum and actinomyces viscosus. serum samples were taken every 2-4 weeks over a 4-month period, whic ... | 1997 | 9467388 |
| evidence for serum immunoglobulin g (igg) antibody responses in macaca fascicularis identified by monoclonal antibodies to human igg subclasses. | this investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin g (igg) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, macaca fascicularis. we subsequently utilized this cross-reactivity to document the characteristics of igg subclass antibody responses in m. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tet ... | 1997 | 9467387 |
| successful treatment of primary actinomyces viscosus endocarditis with third-generation cephalosporins. | 1998 | 9455552 | |
| anaerobic killing of oral streptococci by reduced, transition metal cations. | reduced, transition metal cations commonly enhance oxidative damage to cells caused by hydroperoxides formed as a result of oxygen metabolism or added externally. as expected, the cations fe2+ and cu+ enhanced killing of streptococcus mutans gs-5 by hydroperoxides. however, unexpectedly, they also induced lethal damage under fully anaerobic conditions in a glove box with no exposure to o2 or hydroperoxides from initial treatment with the cations. sensitivities to anaerobic killing by fe2+ varied ... | 1998 | 9435058 |
| lymph node abscess due to actinomyces viscosus in a cat. | in a four-year-old male cat, a subcutaneous phyma about 3.5 cm in diameter was surgically removed from the left inframandibular region. histopathologically, the phyma was found to be the swollen medial retropharyngeal lymph node containing an actinomycotic abscess. the filamentous organisms in the abscess stained positively by the gram's, grocott's and periodic acid-schiff methods, and were negative by the ziehl-neelsen method. by the immunoperoxidase method, the organisms were specifically iden ... | 1997 | 9409531 |
| clinical and microbiological effects of minocycline-loaded microcapsules in adult periodontitis. | clinical and microbiological effects of subgingival delivery of 10% minocycline-loaded (mc), bioabsorbable microcapsules were examined in 15 adult periodontitis patients. patients received oral hygiene instruction 2 weeks prior to the study. at baseline (day 0) all teeth received supragingival scaling (sc); 2 quadrants received no further treatment and 1 quadrant received subgingival scaling and root planning (srp). in the fourth quadrant, the tooth with the deepest probing sites (at least 1 sit ... | 1997 | 9407404 |
| an immunohistochemical study on the localization of porphyromonas gingivalis, campylobacter rectus and actinomyces viscosus in human periodontal pockets. | the localization and distribution of porphyromonas gingivalis, campylobacter rectus and actinomyces viscosus were studied in human periodontal pockets. after obtaining voluntary consent from 9 patients, 12 teeth and their surrounding periodontal tissue with advanced adult periodontitis were extracted carefully so as not to change the structure of the periodontal pockets. the specimens were processed into serial sections. one of the sections was stained with brown & brenn-modified gram stain to o ... | 1997 | 9401932 |
| enzymatic removal and disinfection of bacterial biofilms. | model biofilms of staphylococcus aureus, staphylococcus epidermidis, pseudomonas fluorescens, and pseudomonas aeruginosa were made on steel and polypropylene substrata. plaque-resembling biofilms of streptococcus mutans, actinomyces viscosus, and fusobacterium nucleatum were made on saliva-coated hydroxyapatite. the activity of enzymes against bacterial cells in biofilm was measured by fluorescence microscopy and an indirect conductance test in which evolution of carbon dioxide was measured. glu ... | 1997 | 9293025 |
| isolation of actinomyces viscosus strain ga: characteristics of its cellular biological retardant(s). | actinomyces viscosus strain ga produces an exocellular biological retardant(s) that prevents certain vegetable plants from becoming overgrown. the biological retardant(s) was assayed using the etiolated wheat coleoptile assay, and fractionation of culture supernatant fluid resulted in a partial purification of the retardant(s). the biological retardant(s) was most active around ph 7 in the bioassay and when applied to sterile soil mixture. the biological retardant(s) was tentatively identified a ... | 1997 | 9281860 |
| antimicrobial compounds from ceanothus americanus against oral pathogens. | during the search for antimicrobial compounds from higher plant sources, a methanol extract of ceanothus americanus demonstrated antimicrobial activity against selected oral pathogens. through further bioassay-guided fractionation and purification, three triterpenes (ceanothic acid, 27-hydroxy ceanothic acid and ceanothetric acid) and two flavonoids (maesopsin and maesopsin-6-o-glucoside) were identified. among these, ceanothetric acid and maesopsin-6-o-glucoside were new compounds. ceanothic ac ... | 1997 | 9276981 |
| caries and plaque inhibition in rats by five topically applied dentifrices. | the effectiveness of 5 different brands of dentifrices on caries and plaque were tested in rats in 2 experiments. 10 litters of rats each were infected with streptococcus sobrinus omz-176 and actinomyces viscosus ny1. the rats were offered the diet 2000a containing either 40% of sucrose (study i) or 10% of sucrose (study ii). the following products were tested topically: (1) h2o, (negative control); (2) chlorhexidine diacetate, (positive control); (3) parodontax nf; (4) meridol; (5) colgate tota ... | 1997 | 9249193 |
| [antimicrobial properties of flos lonicerae against oral pathogens]. | the antimicrobial effect of flos lonicerae on oral pathogens was studied. the results showed that 73.9% of the tested pathogens were inhibited at a concentration below 6.25mg/ml. streptococci mutants, actinomyces viscosus and bacteroides melaninogenicus were comparatively more sensitive to flos lonicerae. | 1996 | 9208561 |
| inhibition of a porphyromonas gingivalis colonizing factor between actinomyces viscosus atcc 19246 by monoclonal antibodies against recombinant 40-kda outer-membrane protein. | 1. porphyromonas gingivalis, an important pathogen in human periodontal disease, aggregates with actinomyces viscosus atcc 19246. 2. monoclonal antibodies (mabs) against purified recombinant 40-kda outer-membrane protein (r40-kda, omp) of p. gingivalis 381 inhibited its coaggregation with a. viscosus atcc 19246 in a dose-dependent manner. 3. five mab clones against r40-kda omp were selected. the isotype of the five was igg1. 4. pg-ompa2 inhibited the coaggregation of several strains of p. gingiv ... | 1997 | 9184801 |
| transfection of actinomyces spp. by genomic dna of bacteriophages from human dental plaque. | bacteriophages that produced turbid or clear zones of lysis in strains of actinomyces were isolated from 22 of 124 samples of fresh human dental plaque. all human and nonhuman strains of actinomyces viscosus or actinomyces naeslundii tested in this study were sensitive to infection by one or more of these phages. in contrast, none of the actinomyces odontolyticus, actinomyces israelii, or actinomyces bovis strains tested were susceptible. results of restriction endonuclease analyses indicated th ... | 1997 | 9169205 |
| age-related changes in salivary antibodies to commensal oral and gut biota. | the prevalence of mucosally derived infections appears to increase with age, suggesting dysfunction at the mucosal surfaces. the present investigation was undertaken to examine any age-related changes in secretion rates and concentrations of secretory antibodies in whole and parotid saliva in a healthy adult population. a total of 116 subjects were subdivided into the following age groups: 20-39 years, 40-59 years, 60-79 years and 80 years and over. specific immunoglobulin a (iga), igg and igm a ... | 1997 | 9151645 |
| saliva mediated adherence, aggregation and prevalence in dental plaque of streptococcus mutans, streptococcus sanguis and actinomyces spp, in young and elderly humans. | salivary components in the pellicle mediate bacterial adherence to the tooth. such components may also aggregate bacteria in saliva and prevent them becoming established in dental plaque. in the present study, the adherence and aggregation of streptococcus mutans strain ingbritt, s. sanguis strain 10556 and actinomyces viscosus-strain 19246 mediated by parotid and whole saliva from groups of young and elderly people were examined. significant differences were found between test strains, salivary ... | 1996 | 9134102 |
| experimental model for porphyromonas gingivalis infection in animals. | a virulence model suitable for studying the dynamics of porphyromonas gingivalis infection, including the pathogenicity of p. gingivalis in experimentally induced infections of multiple organs was developed using mouse and hamster. virulence of p. gingivalis strains was expressed contrastingly in subcutaneous (sc) infection in the murine abscess model (mam) and the hamsters abscess model (ham). subcutaneous infection in the mam was characterized by a gravity abscess, spreading from the primary s ... | 1996 | 9110052 |
| incorporation of antibacterial monomer mdpb into dentin primer. | the polymerizable monomer methacryloyloxydodecylpyridinium bromide (mdpb) shows antibacterial activity when immobilized in a resin-based material. in this study, the antibacterial effect of a dentin primer incorporating mdpb was investigated. the influence of incorporation of mdpb on bond strength to dentin and on the curing performance of the adhesive system was also evaluated. experimental primers were prepared by addition of mdpb into a proprietary primer at 1, 2, or 5%. antibacterial effects ... | 1997 | 9109826 |
| anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence. | aiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis. 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. the pulp chamber was opened under aseptic conditions and samples of the root canal content were collected with sterile absorbent paper points, which were placed and dispersed in test tubes containing reduced transport medium rtt. aliquots were dr ... | 1996 | 9028199 |
| plaque ph and microflora of dental plaque on sound and carious root surfaces. | given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ... | 1996 | 9003238 |
| effect of antibodies on the chain length and growth of streptococcus sobrinus. | passive immunization has been suggested as a method to prevent colonization of teeth by mutans streptococci. however, the mechanism of action of antibodies, both polyclonal and monoclonal, is not clear. in this study we investigated the effect of polyclonal antibodies (pabs) and a monoclonal antibody (omvu10) on the chain length and growth of streptococcus sobrinus. during growth in broth s. sobrinus formed significantly longer chains in the presence of pabs in comparison to pre-immune serum (p ... | 1997 | 8955992 |
| a flexible model for the cell wall polysaccharide of streptococcus mitis j22 determined by three-dimensional 13c edited nuclear overhauser effect spectroscopy and 13c-1h long-range coupling constants combined with molecular modeling. | we report on the conformation of a tetrasaccharide fragment in the repeating subunit of the cell wall polysaccharide of streptococcus mitis j22, a receptor for the lectin of actinomyces viscosus t14v in a bacterial coaggregation that is important in the ecological interactions of oral bacteria. although there is considerable overlap of the 1h-nmr signals, some cross peaks can be extracted from conventional two-dimensional nuclear overhauser effect spectroscopy (noesy) data on the polysaccharide. ... | 1996 | 8906970 |
| compounds from syzygium aromaticum possessing growth inhibitory activity against oral pathogens. | a crude meoh extract of syzygium aromaticum (clove) exhibited preferential growth-inhibitory activity against gram-negative anaerobic periodontal oral pathogens, including porphyromonas gingivalis and prevotella intermedia. by means of bioassay-directed chromatographic fractionation, eight active compounds were isolated from this extract and were identified as 5,7-dihydroxy-2-methylchromone 8-c-beta-d-glucopyranoside, biflorin, kaempferol, rhamnocitrin, myricetin, gallic acid, ellagic acid, and ... | 1996 | 8904847 |
| serum antibodies to commensal oral and gut bacteria vary with age. | this study examined the relationship between serum antibody levels to selected bacteria from the commensal oral and gut flora with increased age in a healthy adult population. a total of 116 healthy subjects were studied consisting of the following age groups: 20-39 years (group a), 40-59 years (group b), 60-79 years (group c) and 80+ years (group d). only significantly lower mean igm antibody levels to streptococcus mutans strain guy's serotype c were observed in older age groups (p < 0.001). w ... | 1996 | 8871114 |
| [adherent mechanism of the mutans of actinomyces viscosus t14v]. | three mutans of actionmyces viscous t14v lacking type1 (5951), type 2 (5519) and both types of fimbriae (147) were selected in this study. we compared the ability of their adsorption to the test-tube wall, the result of the cellular coaggregation between these mutant strains and streptococcus sanguis 34, streptococcus sanguis black bova, streptococcus mutans ingbritte, streptococcus sobrinus 6715 and analysed the chemical characterization of cell walls of the three mutans. we found that the spec ... | 1995 | 8762533 |
| effects of actinomyces amphiphile on the fluidity of endothelial cells: a spin label study. | actinomyces amphiphile (aca) is an amphipathic molecule produced by actinomyces viscosus that exhibits several biological activities. the effect of aca on the fluidity and permeability of the plasma membrane in human umbilical vein endothelial cells was analyzed by a spin label method with 5- and 16-stearic acid nitroxide labels (sal). these labels help to visualize the fluidity at the shallow (5-sal) and deep (16-sal) portions of the lipid bilayer. cells were incubated with and without aca (con ... | 1996 | 8708128 |
| oral bacterial attachment to titanium surfaces: a scanning electron microscopy study. | despite the wide use of dental implants, the understanding of the mechanism(s) of bacterial attachment to implant surfaces and of the factors that affect such attachment is limited. in this study, the attachment of oral bacteria--including streptococcus sanguis, actinomyces viscosus, and porphyromonas gingivalis--to titanium (ti) discs with different surface morphology (smooth, grooved, or rough) was examined by scanning electron microscopy (sem). the most bacterial attachment was observed on th ... | 1995 | 8699514 |
| salivacin 140, a novel bacteriocin from lactobacillus salivarius subsp. salicinius t140 active against pathogenic bacteria. | fifteen of 353 environmental isolates of lactic acid bacteria consistently showed activity against listeria monocytogenes, streptococcus mutans, actinomyces viscosus, and/or propionibacterium acnes. strain t140, isolated from the surface of japanese pampas grass leaves and identified as lactobacillus salivarius subsp. salicinius, also had activity against several lactobacillus species, staphylococcus aureus and yersinia enterocolitica. since the antagonistic factor(s) produced by t140 was sensit ... | 1996 | 8695066 |
| enhanced bactericidal activity of arm and hammer dental care. | to compare and contrast antibacterial activities of a baking soda-containing dentifrice, arm and hammer dental care (ahdc) with two fluoride dentifrices without baking soda (crest and colgate). | 1995 | 8695008 |
| construction and preliminary characterization of three hemagglutinin mutants of porphyromonas gingivalis. | targeted insertional mutagenesis was used to construct haga, hagb, and hagc hemagglutinin mutants of porphyromonas gingivalis. pjrd215-derived plasmids containing teta(q)2 and portions of the targeted genes were conjugated into p. gingivalis. interruption of the three loci was confirmed by southern hybridization, sequencing, reverse transcription-pcr, and microtiter hemagglutination assays. no significant differences in hydrophobicity or coadherence to actinomyces viscosus were detected between ... | 1996 | 8606121 |
| inhibition of adherence of actinomyces naeslundii (actinomyces viscosus) t14v-j1 to saliva-treated hydroxyapatite by a monoclonal antibody to type 1 fimbriae. | a monoclonal antibody to actinomyces naeslundii (a. viscosus) t14v-j1 type 1 fimbriae, capable of inhibiting the adherence of this bacterium to salivary proline-rich protein-treated hydroxyapatite, was generated by immunization of swr mice with a. naeslundii 55-19, a strain derived from t14v-j1 that possess only type 1 fimbriae. supernatants of hybridomas were screened for reactivity with purified type 1 fimbriae. an igg monoclonal antibody, 86-49e, blocked the adsorption of the parent strain to ... | 1996 | 8604255 |
| cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis. | the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ... | 1995 | 8602339 |
| microbial induction of dentinal caries in human teeth in vitro. | the ability of lactobacillus casei, streptococcus sobrinus, actinomyces viscosus, and streptococcus salivarius to induce dental caries was determined in vitro. a class i cavity (depth: 2mm) was prepared in extracted human caries-free premolars to make dentin blocks. the blocks were inoculated with these four bacterial strains in a monoinfective fashion and were incubated under anaerobic conditions. in addition to the monoinfection groups, mixed-infection groups of l. casei with s. sobrinus or a. ... | 1995 | 8601764 |
| cervicofacial actinomyces viscosus infection in a brazilian fila: a case report and literature review. | a five-month-old, male brazilian fila presented with a three-day history of a focal swelling in the left superior palpebra and a focal, subcutaneous swelling over the dorsal cervical region. both lesions initially responded to warm compresses and a two-week course of oral amoxicillin-clavulanic acid therapy. the eyelid swelling recurred after discontinuation of the oral antibiotic therapy. the lesion was progressive and was refractory to trimethoprim-sulfadiazine therapy. culture and sensitivity ... | 1995 | 8581545 |
| experimental root surface caries in hamsters the development of the disease after inoculations of two types of cariogenic bacteria. | the aim of this study was to investigate the development of root surface caries in hamsters fed a high-sucrose diet over a 24-week period after inoculations of two types of cariogenic bacteria. twenty-one day old male golden hamsters (n = 103) were divided into 5 groups. four groups were given diet 2000, and one group was given a stock diet ce-2, of the groups given diet 2000, three groups were infected with actinomyces viscosus atcc 15987 and streptococcus mutans ntcc 10449 separately (av and s ... | 1995 | 8556786 |
| the effect of various fluoride compounds on the development of experimental root surface caries in hamsters. | the aim of this investigation was to evaluate the effect of topical applications of various fluoride compounds on the development of root surface caries in hamsters. male golden hamsters (n = 115) were divided into 7 groups and were given a caries-promoting diet. six groups were infected with actinomyces viscosus atcc 15987; and to 5 infected groups, distilled water (dw) and solutions containing 500 ppmf of acidulated-phosphate fluoride (apf), stannous fluoride (snf2), copper fluoride (cuf2), an ... | 1995 | 8556785 |
| comparison of the distribution of actinomyces in dental plaque on inserted enamel and natural tooth surfaces in periodontal health and disease. | the distribution of actinomyces naeslundii, actinomyces viscosus and actinomyces odontolyticus in healthy and diseased adult populations was studied in 3 different ways. first, supragingival plaque formation at 2 through 72 h was examined in 12 periodontally healthy adults using a removable pre-measured surface of enamel bonded to molars and premolars. second, a cross-sectional examination of the composition of both supragingival and subgingival plaque of unknown age was conducted in 205 adults ... | 1993 | 8510984 |
| dental plaque development on defined streptococcal surfaces. | coaggregations between bacterial species have been widely studied in vitro but not in the mouth. a new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. chips were covered with streptococcus oralis atcc 10557, which coaggregated with many oral species, or streptococcus gordonii s7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. there were no di ... | 1993 | 8510979 |
| sensitisation of cariogenic bacteria to killing by light from a helium-neon laser. | suspensions of the cariogenic bacteria streptococcus mutans, s. sobrinus, lactobacillus casei and actinomyces viscosus were exposed to light from a 7.3-mw helium-neon laser in the presence of toluidine blue o. a substantial killing rate (c. 10(6) cfu) of all four species was achieved with a dye concentration of 50 micrograms/ml and a light energy dose of 33.6 j/cm2. this was achieved in 60 s, an exposure time that is clinically acceptable. exposure to laser light in the absence of the dye did no ... | 1993 | 8510132 |
| induction of inflammatory cytokines by a soluble moiety prepared from an enzyme lysate of actinomyces viscosus cell walls. | a carbohydrate-rich and immunobiologically active component, m1av, was prepared from an m-1 endo-n-acetylmuramidase digest of cell walls of actinomyces viscosus atcc 19246 by cm sephadex c-25 and sephadex g-100 chromatography. m1av stimulated thioglycolate-induced peritoneal macrophages from c3h/hen and c3h/hej mice to release cell-free tumour necrosis factor (tnf) and interleukin (il)-6, and a cell-associated thymocyte activating factor, probably il-1. an intravenous (i.v.) injection of m1av in ... | 1993 | 8510131 |
| [a case of chronic actinomycosis in a carcinomatous lesion]. | a gram positive, catalase-positive actinomyces viscosus strain has been isolated from cervicofascial carcinomatous lesion. identification of this human strain of actinomyces viscosus was carried out with biochemical tests. tests for catalase, indole, nitrate reduction, methyl red reaction acetoin (vp) and gelatin hydrolysis were done by the procedure recommended by the technique described by grencser and slack. carbohydrate fermentation tests were done with three basal media: actinomyces ferment ... | 1993 | 8502184 |
| simultaneous caries induction and calculus formation in rats. | weanling specific pathogen-free osborne-mendel rats were fed a high-calcium, high-phosphorus diet with various levels of sucrose and inoculated with streptococcus sobrinus strain 6715-13wt and actinomyces viscosus strain omz-105 in order to determine whether calculus and caries could develop simultaneously. rats consumed diets designated rc-16-5, rc-16-25, or rc-16-50 which partially replaced the corn starch component with progressively higher levels of sucrose, thus, to 5, 25, or 50% sucrose. i ... | 1993 | 8501282 |
| adhesion of actinomyces isolates to experimental pellicles. | the ability of oral bacteria to adhere to surfaces is associated with their pathogenicity. actinomyces can adhere to pellicle and cells through extracellular fimbriae. research on adhesion of actinomyces has been conducted with use of hydroxyapatite (ha) coated with mammalian-derived salivary constituents, whereas the bacterial-derived components of the acquired pellicle have been largely ignored. the influence of the cell-free bacterial enzyme, glucosyltransferase (gtf), on adhesion of human an ... | 1993 | 8496474 |
| coaggregation of prevotella intermedia with oral actinomyces species. | five strains of prevotella intermedia were examined for their ability to coaggregate with various gram-positive and gram-negative species of oral bacteria. two of the p. intermedia strains coaggregated with selected actinomyces species, p. intermedia 27 with actinomyces viscosus t14v and actinomyces naeslundii atcc 12104, pk606, pk984, and pk947, and p. intermedia 113 with actinomyces odontolyticus wvu 1546 and actinomyces israelii wvu 838. exposure of both prevotella strains but not the actinom ... | 1993 | 8478088 |
| synergistic, growth-inhibitory effects of chlorhexidine and copper combinations on streptococcus mutans, actinomyces viscosus, and actinomyces naeslundii. | the purpose of this investigation was to determine whether chlorhexidine combined with copper would exert a synergistic, growth-inhibitory effect against selected oral bacteria. actinomyces viscosus, actinomyces naeslundii, and streptococcus mutans were all susceptible to chlorhexidine individually, with s. mutans displaying the highest sensitivity. much higher concentrations of copper were needed to achieve growth inhibition of the micro-organisms tested. determination of minimum inhibitory con ... | 1993 | 8423250 |
| complete nucleotide sequence of the actinomyces viscosus t14v sialidase gene: presence of a conserved repeating sequence among strains of actinomyces spp. | the nucleotide sequence of the actinomyces viscosus t14v sialidase gene (nanh) and flanking regions was determined. an open reading frame of 2,703 nucleotides that encodes a predominately hydrophobic protein of 901 amino acids (m(r), 92,871) was identified. the amino acid sequence at the amino terminus of the predicted protein exhibited properties characteristic of a typical leader peptide. five 12-amino-acid units that shared between 33 and 67% sequence identity were noted within the central do ... | 1993 | 8418033 |
| glucose stimulates camp accumulation in the oral bacterium actinomyces viscosus. | actinomyces viscosus t14v, a gram-positive bacterium found in the oral cavity, was found to be insensitive to glucose-mediated catabolite repression. basal levels of beta-galactosidase (18-26 u) were observed at all phases of growth regardless of the culture conditions. further, beta-galactosidase could not be induced with lactose, or with a known inducer of the enzyme, isopropyl-beta-d-thiogalactoside, or with dibutyryl camp. glucose, on the other hand, stimulated camp accumulation in a concent ... | 1993 | 8395889 |
| effect of longovital treatment on development of periodontal disease in rats. | longovital is a herbal tablet with documented immunostimulatory effects in man. in the present study the effect of longovital on development of periodontal disease was investigated in a rat model. fifty-four conventional rats, 5 wk old, were distributed into the following groups: a) untreated, uninfected; b) untreated, infected with actinomyces viscosus and porphyromonas gingivalis wk 8; c) treated with longovital 80 mg x 3/wk, wk 5-14, uninfected; d) treated with longovital 80 mg x 3/wk, wk 5-1 ... | 1993 | 8382835 |
| effect of co-aggregation on the pathogenicity of oral bacteria. | the pathogenicity of oral bacteria was studied by measuring the development of subcutaneous abscesses in mice after infection with actinomyces viscosus and streptococcus mitis either singly or as co-aggregated pairs. heat-treated cells were also tested. the pathogenicity of the co-aggregates was examined in various viable and heat-treated combinations of the two bacterial species. more abscesses were formed by a. viscosus than s. mitis at all the bacterial concentrations tested. also, abscess fo ... | 1993 | 8366516 |
| in vitro demineralization by strains of actinomyces viscosus and streptococcus sobrinus of sound and demineralized root surfaces. | root sections were inoculated with one of two strains of actinomyces viscosus or a strain of streptococcus sobrinus and then incubated for 9 days in trypticase soy broth (tsb) containing 0.25% glucose or tsb supplemented with 0.25% sucrose (tsb+s). lesion progression was measured from microradiographs. one section from each group was examined with a transmission electron microscope. lesion progression was associated with a fall in the ph of the medium. however, lesion progression was not correla ... | 1993 | 8360359 |
| characterization of actinomyces with genomic dna fingerprints and rrna gene probes. | cellular dna from 25 actinomyces naeslundii and actinomyces viscosus strains belonging to the 7 taxonomic clusters of fillery et al. (1978) and several unclustered strains was obtained by enzymatic and n-lauroylsarcosine/guanidine isothiocyanate treatment of whole cells, followed by extraction of the nucleic acid. the dna samples were digested with restriction endonucleases bamhi or pvuii, and agarose gel electrophoresis was used to obtain dna fingerprints. the dna fragments were subjected to so ... | 1993 | 8360358 |
| the effect of arginine on glycolysis by streptococcus mutans and actinomyces viscosus. | 1993 | 8359461 | |
| actinomyces species associated with intrauterine contraceptive devices and pelvic inflammatory disease. | swabs from 112 removed intrauterine contraceptive devices (iucd), endocervical swabs from these women and from 65 women with pelvic inflammatory disease (pid) were studied for actinomyces, using direct fluorescent antibody test and culture. endocervical swabs from 50 control subjects were also studied. actinomyces species could be detected in 23 (20.5 percent) of iucd wearers and 8 (12.3 percent) of patients with pid. in control cases, no actinomyces were detected. the isolation rate using a sel ... | 1993 | 8300169 |
| correlation between thermal sensitivity and microorganisms isolated from deep carious dentin. | the correlation between thermal sensitivity and the microorganisms present in 29 deep carious lesions was studied. the numbers of lactobacilli and total gram-positive rods in the carious lesions were found to be negatively related to the length of pain triggered by cold and heat stimulants. the presence of gram-positive cocci and non-black-pigmented bacteroides were positively associated with both cold and heat sensitivities. black-pigmented bacteroides, streptococcus mutans, and total anaerobic ... | 1993 | 8289023 |
| production and characterization of monoclonal antibodies against bacterial lectin of eikenella corrodens. | a lectin-like substance (ecls) was purified from the eikenella corrodens 1073 cell and monoclonal antibodies were produced against it to confirm the role of ecls in adhesive properties of e. corrodens such as hemagglutination and coaggregation with oral bacteria. four hybridoma clones were selected. two of the antibodies were of the igg1 isotype and the others were of the igg2b isotype. these monoclonal antibodies inhibited both the hemagglutination of e. corrodens and the coaggregation with act ... | 1993 | 8254457 |
| interaction of the salivary glycoprotein ep-gp with the bacterium streptococcus salivarius hb. | the interaction of the human salivary glycoprotein ep-gp with a number of oral bacterial species, following incubation with human whole saliva, has been investigated. ep-gp could be detected with a specific monoclonal antibody, by means of elisa or by electrophoresis in combination with western transfer. the results indicated that ep-gp is bound only by streptococcus salivarius, and not by the other tested strains of bacteria, actinomyces viscosus, a. naeslundii, actinobacillus actinomycetemcomi ... | 1993 | 8254122 |
| antibacterial activity of dentinal bonding agents. | the susceptibility of five bacterial species to seven dentinal bonding agents was examined in vitro. agar diffusion tests using filterpaper disks containing 10 microl each of conditioner, primer, or resin were performed on blood agar and mitis salivarius bacitracin agar. chlorhexidine (0.2%) was used as a positive control. after incubation, zones of inhibited bacterial growth were measured. of all the compounds tested, gluma cleanser and gluma etchant showed the strongest growth inhibition for a ... | 1993 | 8210322 |
| an in vitro model for adhesion of bacteria to human tooth root surfaces. | a model mimicking bacterial colonization of dentine has been developed. it employs uniform particles of pulverized human tooth root tissue incubated with radioactively labelled bacteria. after incubation, the number of attaching bacteria is quantified. attachment of streptococcus mutans ua140, actinomyces viscosus t14, and lactobacillus casei 101 was found to be time dependent and complete within 1-3 h. dissociation constants (kd) of the interactions equalled 2.5 x 10(8) and 1.6 x 10(8) cells/ml ... | 1994 | 8185501 |
| adherence of oral microorganisms to guided tissue membranes: an in vitro study. | microorganisms can adhere and colonize on an exposed guided tissue regeneration (gtr) membrane thus developing a nidus of infection. the purpose of this study was to compare early bacterial adhesion to three different gtr membranes. expanded polytetrafluoroethylene, polyglactin 910, and collagen were used as the test membranes. in part i of this study 15 different oral microbes were used to compare their relative ability to adhere to the membranes. six of the most strongly adherent bacteria (act ... | 1994 | 8164114 |
| relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults. | to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ... | 1994 | 8157769 |
| cellular fatty acid composition and phenotypic and cultural characterization of cdc fermentative coryneform groups 3 and 5. | seventy strains of fermentative, asporogenous, gram-positive coccobacilli or short rods form two closely related groups which have been designated cdc fermentative coryneform groups 3 (32 strains, xylose fermenters) and 5 (38 strains, xylose nonfermenters). the two taxa are otherwise similar to each other phenotypically and culturally and by a distinctive staphylococcus-like odor and by cellular fatty acid (cfa) composition. cdc group 3 and cdc group 5 strains have been isolated from clinical so ... | 1994 | 8051247 |
| transformation of actinomyces spp. by a gram-negative broad-host-range plasmid. | the gram-negative broad-host-range vector pjrd215 was transferred by electroporation into strains of actinomyces viscosus or actinomyces naeslundii at efficiencies which ranged from 10(2) to 10(7) transformants per microgram of plasmid dna. the actinomyces transformants expressed pjrd215-encoded resistance to kanamycin and streptomycin. moreover, the transforming plasmid dna had not undergone any deletions or rearrangements, nor had it integrated into the genomes of these strains. | 1994 | 8021199 |
| [brain abscess complicating dental caries in children]. | brain abscess following dental or periapical infection is rare in childhood. this report describes brain abscesses found in two children with dental caries. case 1.--a 12 year-old boy was admitted because he had suffered from acute meningitis for 3 days. clinical examination showed symptoms of meningitis plus palsy of the right third and fourth cranial nerves and of the left facial nerve, and a defect in the left temporal field. funduscopic examination showed papilledema; ct scan and mri showed ... | 1994 | 7987445 |
| an indirect fluorescent antibody technique for detection of anti-dermatophilus congolensis antibodies in sheep. | an indirect fluorescent antibody (ifa) technique has been developed for detection of anti-dermatophilus antibodies in sheep. sera from 25 bacteriologically confirmed clinically affected sheep and from 10 negative non affected lambs were used. whole cell antigen from brain heart infusion cultures of d. congolensis was used and all sera were tested in the same way for cross-reactivity against antigens obtained from cultures of actinomyces viscosus, micrococcus luteus, nocardia asteroides, and cory ... | 1994 | 7941032 |
| [actinomyces viscosus in subdural empyema]. | the authors describe the finding of actinomyces viscosus in pus from a subdural empyema in a child patient. when searching for the probable primary source of this uncommon infection of the central nervous system the authors identified by cultivation the same bacterial strain in a periapical dental granuloma. bacterial diagnosis and radical surgical and antimicrobial therapy led to normalization of a clinical condition with an initially unfavourable prognosis. | 1994 | 7909500 |
| fimbria damage and removal of adherent bacteria after exposure to acoustic energy. | the physical effects of low-frequency acoustic energy on actinomyces viscosus were studied with electron microscopy to explore both acoustically induced damage to fimbriae on the surface of these bacteria and acoustic removal of bacteria from saliva-treated hydroxyapatite disks. a bacterial suspension was exposed to acoustic energy from a laboratory acoustic generator (50 kpa, 200 hz) and from a new electronic toothbrush, the sonicare. the exposed bacteria were examined with electron microscopy ... | 1993 | 7903443 |
| the antimicrobial activity of prevention mouthrinse. | prevention mouthrinse was designed to serve as a vital supplement to normal oral hygiene procedures. to determine the antimicrobial potency of this mouthrinse, minimal inhibitory concentrations (mics), bactericidal kinetics, and short-term exposure studies were conducted. a spectrum of oral microorganisms was employed in this investigation: streptococcus sanguis, streptococcus anginosus, streptococcus mutans, actinomyces viscosus, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, ei ... | 1993 | 7880467 |
| initial catabolism of sorbitol in actinomyces naeslundii and actinomyces viscosus. | the initial steps of sorbitol catabolism were studied in 4 strains of actinomyces naeslundii and actinomyces viscosus that had been isolated from human dental plaque. cell-free extracts were prepared from cells grown in the presence of either sorbitol, xylitol or glucose. the extracts from all strains grown on sorbitol had nicotinamide adenine dinucleotide-linked dehydrogenase activities for sorbitol and xylitol and reduced nicotinamide adenine dinucleotide-linked reductase activities for fructo ... | 1994 | 7870473 |
| bacterial-protein interactions in the oral cavity. | bacteria in the oral cavity must interact with salivary proteins if they are to survive. such interactions can take several forms, either providing nutrients, a means of adhesion to surfaces, or resulting in aggregation or killing and, therefore, clearance of organisms. recent work has provided an insight into the mechanisms of some of these bacterial-protein interactions, revealing complexity and diversity. for example, the interaction between a putative streptococcus mutans adhesin, p1 (b, i/i ... | 1994 | 7865084 |
| the superoxide dismutase gene, a target for detection and identification of mycobacteria by pcr. | the superoxide dismutase gene has been identified as a target in screening for the presence of mycobacteria on the genus level and differentiating relevant mycobacterial species from one another by pcr. consensus primers deduced from known superoxide dismutase gene sequences allowed the amplification of dnas from a variety of bacteria, fungi, and protozoa. selected amplicons from actinomyces viscosus, corynebacterium diphtheriae, corynebacterium pseudodiphtheriticum, mycobacterium avium, m. fort ... | 1994 | 7852575 |
| quantifying the strength of bacterial adhesive interactions with salivary glycoproteins. | we adapted an assay that has been used to estimate the strength of eukaryotic cell-cell and cell-extracellular matrix adhesive interactions (mcclay et al., 1981) to quantify the strength of bacterial (streptococci, fusobacteria, actinomyces) interactions with salivary receptors. bacteria are centrifuged onto human submandibular/sublingual or parotid-saliva-coated microtiter wells. plates are sealed with pressure-sensitive, double-sided tape which allows them to be inverted and centrifuged again. ... | 1995 | 7790599 |
| bacterial adhesion to dental amalgam and three resin composites. | the ability of three oral bacteria to adhere to hydrophobic amalgam (water contact angle 60 degrees) and hydrophobic resin composites (prisma-ap.h 56 degrees. herculite xrv 82 degrees and z100 89 degrees) was compared using an in vitro assay. | 1995 | 7782529 |
| construction and use of integration plasmids to generate site-specific mutations in the actinomyces viscosus t14v chromosome. | stable transformants of actinomyces viscosus t14v carrying heterologous dna were obtained with the aid of integration plasmids. these plasmids contained a kanamycin resistance (kmr) gene flanked by a. viscosus t14v genomic dna, including parts of the type 1 structural fimbrial subunit gene (fimp) on one or both sides of the antibiotic marker. significantly more kmr transformants were obtained with a plasmid carrying longer segments of homologous strain t14v dna. integration of this plasmid into ... | 1995 | 7622214 |
| bacterial adsorption to fetuin and mucin pellicle. | the ability of actinomyces viscosus (a. viscosus) wvu 627 to attach to hydroxyapatite (ha) beads treated with either bovine fetuin or bovine mucin was studied. all preparations used were blocked with bovine serum albumin prior to incubation with [3h] thymidine-labeled a.viscosus cells. the quantity of fetuin or mucin adsorbed on the ha beads was determined by use of [3h] fetuin or [3h] mucin. approximately 6 micrograms protein of [3h] fetuin and 20 micrograms of protein of [3h] mucin adsorbed to ... | 1995 | 7545741 |
| killing of cariogenic bacteria by light from a gallium aluminium arsenide diode laser. | suspensions of streptococcus mutans, s. sobrinus, lactobacillus casei and actinomyces viscosus were exposed to light from a gallium aluminium arsenide laser in the presence of aluminium disulphonated phthalocyanine and the numbers of survivors determined. exposure to the laser light in the absence of the dye, or the dye in the absence of the laser light, had no significant effect on the viability of the organisms. however, a light-dose-related decrease in the viable count of all four target orga ... | 1994 | 7525674 |
| antibacterial action of dental cements: an in vitro study. | the antibacterial activity of seven commercially available dental cements (eugespad, dentical, dycal, expaliner, pr. scell, pr. base cement, pr. lining cement) against 1) bacterial species implicated in carious lesions or in dental plaque (actinomyces israelii atcc 10048, actinomyces viscosus atcc 19246, streptococcus mutans atcc 25175, streptococcus sanguis atcc 10557) and 2) bacterial samples of stimulated saliva was studied, in vitro, using a modification of the method of mccomb and ericson ( ... | 1995 | 7492899 |
| bacteria in human mouths involved in the production and utilization of hydrogen peroxide. | earlier studies have demonstrated that pure cultures of oral streptococci produce hydrogen peroxide but none has found any free peroxide in dental plaque or salivary sediment despite streptococci being major components of their mixed bacterial populations. the absence of peroxide in plaque and sediment could be due to the dominance of its destruction over its formation by bacterial constituents. to identify which of the oral bacteria might be involved in such a possibility, pure cultures of 27 d ... | 1995 | 7487577 |
| the role of the succinate pathway in sorbitol fermentation by oral actinomyces viscosus and actinomyces naeslundii. | the sorbitol fermentation by actinomyces viscosus and actinomyces naeslundii was studied with washed sorbitol-grown cells. the fermentation was followed by titration of acids produced at ph 7.0 under anaerobic conditions. metabolic end-products and intracellular levels of nad, nadh and glycolytic intermediates during the fermentation were also analyzed. cell extracts were examined for certain enzyme activities. bicarbonate was required for acid production from sorbitol and from a mixture of gluc ... | 1994 | 7478761 |
| in vitro modulation of oral bacterial adhesion to saliva-coated hydroxyapatite beads by milk casein derivatives. | bovine caseinate, derivatives of its glycosylated moiety [caseinoglycomacropeptide (cgp)], and caseinophosphopeptides were evaluated as inhibitors of adhesion of oral bacteria to saliva-coated hydroxyapatite beads (s-ha). all milk casein-derived components behaved as potent inhibitors of streptococcus sanguis omz 9 and streptococcus sobrinus omz 176 adhesion to s-ha, whereas neither bovine serum albumin nor polyethyleneglycol were able to interfere with the adhesion of these strains. by contrast ... | 1994 | 7478758 |
| use of the syrian golden hamster for the induction of intraoral abscesses by sutures contaminated with human subgingival plaque. | analysis of normal oral flora in 150 cheek pouches of hamsters (mesocricetus auratus) defined the microbial working environment and demonstrated the absence of human oral black-pigmented bacteria. silk sutures saturated with porphyromonas gingivalis, prevotella intermedia or subgingival plaque were used to close wounds made in hamster's cheek pouches. abscesses were formed when sutures had solitary p. gingivalis or other bacteria mixed with p. gingivalis or when p. intermedia was mixed with othe ... | 1994 | 7478755 |
| growth-inhibitory effect of pyrophosphate on oral bacteria. | the purpose of this investigation was to determine whether pyrophosphate, the anticalculus component of tartar-control dentifrices, exerts antimicrobial activity against oral bacteria commonly found in supragingival plaque. minimal inhibitory concentrations of pyrophosphate were determined for streptococcus sanguis, streptococcus mutans (serotype c), actinomyces viscosus and actinomyces naeslundii. all of the bacteria tested were susceptible to pyrophosphate with identical minimal inhibitory con ... | 1994 | 7478751 |
| infections caused by actinomyces viscosus. | two cases of actinomyces viscosus infection of the lungs were seen in nonimmunosuppressed patients. one patient had a peripheral actinomycotic lung mass resembling a tumor. both patients responded to a long course of penicillin therapy. reports of a. viscosus infections are rare, although the organism colonizes the mouths of most adult humans. only ten cases have previously been described. there is no characteristic of a. viscosus infection that can distinguish it from actinomyces israelii or ac ... | 1981 | 7457420 |
| modulation of immunoreactivity to periodontal disease-associated microorganisms during pregnancy. | the lymphocyte blastogenic response to a panel of antigens and mitogens was assessed in a group of 20 women throughout their pregnancy. in addition, a group of five nonpregnant women was monitored simultaneously to identify variations in response to the same stimulants. the stimulants included orally associated bacterial antigens (streptococcus sanguis, actinomyces viscosus, bacteroides asaccharolyticus, bacteroides melaninogenicus subsp. intermedius, bacteroides [capnocytophaga] ochraceus, and ... | 1980 | 7399691 |
| fibril-mediated adherence of actinomyces viscosus to saliva-treated hydroxyapatite. | fibril-mediated adherence of actinomyces viscosus strain t14v cells to saliva-treated hydroxyapatite was studied. fibrils were purified by ammonium sulfate precipitation and differential centrifugation from the crude supernatant of whole cells that were sheared by one passage through a french pressure cell. purified fibrils and crude supernatant inhibited strain t14v adherence to saliva-treated hydroxyapatite to similar extents. however, anti-strain t14v serum and antifibril specific antibody co ... | 1980 | 7399676 |
| actinomycosis due to actinomyces viscosus in a young dog. | 1980 | 7376388 | |
| effects of metabolic inhibitors on extracellular fructosyltransferase production in actinomyces viscosus. | extracellular fructosyltransferase (levansucrase; ec 2.4.1.10) production in actinomyces viscosus t14av was demonstrated to occur concomitantly with cellular growth. the inhibition of both cellular ribonucleic acid and protein synthesis resulted in no further accumulation of enzyme activity. the antibiotic sodium clofibrate differentially inhibited the production of fructosyltransferase by strain t14av. furthermore, the antibiotic preferentially inhibited [14c]acetate incorporation into cellular ... | 1981 | 7333676 |
| potential salicylamide antiplaque agents: in vitro antibacterial activity against actinomyces viscosus. | a series of 55 salicylamides, including 3,5-dibromo-, 5-n-alkyl-, and 5-n-acylsalicyloyl derivatives of various anilines, heterocyclic amines, benzylamines, and alkylamines, was synthesized and evaluated for in vitro antibacterial activity against actinomyces viscosus, an adherent oral microorganism implicated in periodontal disease. the in vitro minimum inhibitory concentrations of 15 4'-bromosalicylanilides were found to correlate (r = 0.92) with estimated log d values. several nonhalogenated ... | 1981 | 7328586 |
| purification and characterization of surface fibrils from taxonomically typical actinomyces viscosus wvu627. | fibrils of actinomyces viscosus wvu627 (numerical taxonomy cluster 1) were obtained by homogenization and purified by ultrafiltration, ammonium sulfate precipitations, gel filtration, and ion-exchange chromatography. electron microscopy and resolution of a single band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis attested to the purity of the preparation. purified fibrils were composed mainly of protein; small quantities of carbohydrate and phosphorus were detected. immunoelectrop ... | 1981 | 7275934 |
| lactose-reversible coaggregation between oral actinomycetes and streptococcus sanguis. | freshly isolated strains of oral actinomycetes were obtained from human dental plaque and were tested for the ability to coaggregate with common laboratory stock strains of streptococcus sanguis. strains belonging to the genera actinomyces, arachnia, bifidobacterium, and bacterionema were isolated. only members of the genus actinomyces coaggregated with the streptococci, and only actinomyces viscosus and actinomyces naeslundii exhibited lactose-reversible interactions. a total of 61 strains, con ... | 1981 | 7263074 |
| regulation of extracellular slime production by actinomyces viscosus. | extracellular slime polysaccharides produced two actinomyces viscosus strains, t14v and t14av, were compared. in various media containing glucose, t14av produced abundant extracellular viscous slime polysaccharide, whereas t14v produced lower levels. furthermore, fractionation of these polysaccharides showed that the two extracellular polysaccharides differed in molecular size and net charge. since there was a significant difference in the relative abilities of chemically defined medium and chem ... | 1981 | 7251159 |
| alveolar bone loss in rats after immunization with actinomyces viscosus. | we investigated a possible cause-and-effect relationship between sensitization against actinomyces viscosus nyl and destructive periodontal disease in ric-sprague-dawley rats. germfree rats (66) were either immunized with a. viscosus nyl (day 20) or orally infected with a. viscosus nyl (days 38 and 39) or both. we measured alveolar bone loss in maxillary and mandibular molars, in vitro t-lymphocyte responsiveness, and serum antibody titers. in immunized and monoassociated rats bone loss in both ... | 1981 | 7228410 |
| effect of bacterial aggregation on the adherence of oral streptococci to hydroxyapatite. | several in vitro assay systems to measure the adherence of human dental plaque bacteria to solid surfaces such as teeth, glass, and hydroxyapatite have been published. in many studies a variety of macromolecular solutes have been used to study the adherence process. often these solutes are able to aggregate the test bacterial and thus may alter the outcome of adherence experiments. in this study, the effects of the aggregation of streptococcus sanguis on adherence to spheroidal hydroxyapatite is ... | 1981 | 7228408 |
| influence of growth medium on adsorption of streptococcus mutans, actinomyces viscosus, and actinomyces naeslundii to saliva-treated hydroxyapatite surfaces. | the influence of the growth medium on the ability of strains of streptococcus mutans, actinomyces viscosus and a. naeslundii to attach to saliva-treated hydroxyapatite (s-ha) surfaces was studied. preliminary experiments indicated that cells of each species harvested in lag, log, and early stationary phases of growth adsorbed comparably to s-ha; thus, early stationary phase cells were used in all subsequent assays. strains were grown in chemically defined medium (cdm), in cdm supplemented with g ... | 1981 | 7216480 |
| differences in the adsorptive behavior of human strains of actinomyces viscosus and actinomyces naeslundii to saliva-treated hydroxyapatite surfaces. | human strains of actinomyces viscosus and a. naeslundii differ in the time of their appearance and in their patterns of colonization in the mouth. strains of these organisms were found to differ in their abilities to adsorb to saliva-treated hydroxyapatite (s-ha) surfaces, thought to mimic the teeth, and these differences parallel their patterns of colonizing the dentition. thus, strains of a. viscosus tended to adsorb in higher numbers to hydroxyapatite (ha) treated with saliva of older childre ... | 1981 | 7216448 |
| selective antibacterial properties of lysozyme for oral microorganisms. | the antibacterial properties of lysozyme were investigated with oral microorganisms representing the seven serotypes (a through g) of streptococcus mutans, veillonella alcalescens, and the virulent (v) and avirulent (av) strains of actinomyces viscosus t14. growth of bacteria in defined medium was monitored spectrophotometrically after the addition of various amounts (25 mug to 5 mg/ml) of enzyme. no growth inhibition of v. alcalescens was observed. inhibition of a. viscosus t14(v) and a. viscos ... | 1980 | 7216430 |