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deciphering the landscape of host barriers to listeria monocytogenes infection.listeria monocytogenes is a common food-borne pathogen that can disseminate from the intestine and infect multiple organs. here, we used sequence tag-based analysis of microbial populations (stamp) to investigate lmonocytogenes population dynamics during infection. we created a genetically barcoded library of murinized lmonocytogenes and then used deep sequencing to track the pathogen's dissemination routes and quantify its founding population (nb) sizes in different organs. we found that the pa ...201728559314
inhibitor-assisted high-pressure inactivation of bacteria in skim milk.the combined inactivation effects of high hydrostatic pressure (hhp) and antimicrobial compounds (potassium sorbate and ε-polylysine [ε-pl]) on 4 different bacterial strains present in skim milk and the effect of these treatments on milk quality were investigated in this study. hhp treatment at 500 mpa for 5 min reduced the populations of escherichia coli, salmonella enterica typhimurium, listeria monocytogenes, and staphylococcus aureus from 6.5 log colony-forming units (cfus) or higher to less ...201728556370
whole genome and core genome multilocus sequence typing and single nucleotide polymorphism analyses of listeria monocytogenes associated with an outbreak linked to cheese, united states, 2013.epidemiological findings of a listeriosis outbreak in 2013 implicated hispanic-style cheese produced by company a, and pulsed-field gel electrophoresis (pfge) and whole genome sequencing (wgs) were performed on clinical isolates and representative isolates collected from company a cheese and environmental samples during the investigation. the results strengthened the evidence for cheese as the vehicle. surveillance sampling and wgs three months later revealed that the equipment purchased by comp ...201728550058
incorporating zataria multiflora boiss. essential oil and sodium bentonite nano-clay open a new perspective to use zein films as bioactive packaging materials.active zein films with different levels of zataria multiflora boiss. essential oil were produced successfully. to enhance properties of this biopolymer for food packaging applications, sodium bentonite clay was used at two levels (2 and 4%). the results indicated that the addition of z. multiflora boiss. essential oil caused a reduction in tensile strength and young's modulus and slight increase in the percent of elongation at break of the films. maximum solubility in water and water vapor perme ...201728549407
erratum: uncovering listeria monocytogenes hypervirulence by harnessing its biodiversity. 201728546578
the absence of tnf permits myeloid arginase 1 expression in experimental l. monocytogenes infection.during an immune response inflammatory macrophages with their wide variety of effector mechanisms including the expression of inducible nitric oxide synthase play an important part in the defense against invading pathogens. the inflammatory phenotype requires the presence of tnf which suppresses alternative activation. in the bacterial listeria monocytogenes infection model inflammatory macrophages are crucial for protection. after infection, tnf-deficient hosts have a similar number of splenic ...201728545808
down regulation of macrophage ifngr1 exacerbates systemic l. monocytogenes infection.interferons (ifns) target macrophages to regulate inflammation and resistance to microbial infections. the type ii ifn (ifnγ) acts on a cell surface receptor (ifngr) to promote gene expression that enhance macrophage inflammatory and anti-microbial activity. type i ifns can dampen macrophage responsiveness to ifnγ and are associated with increased susceptibility to numerous bacterial infections. the precise mechanisms responsible for these effects remain unclear. type i ifns silence macrophage i ...201728542482
invasive listeria monocytogenes gastroenteritis leading to stupor, bacteremia, fever, and diarrhea: a rare life-threatening condition.listeria monocytogenes is a gram-positive, rod-shaped organism that can cause serious infections such as meningitis, invasive gastroenteritis, and endocarditis. every year 1600 people in the united states are affected, with significant mortality of 260 people annually. listeria gastroenteritis has the third highest mortality rate among all the food-borne infection. invasive listeriosis most commonly affect pregnant women, those in extremes of ages, people with comorbid diseases, and people with ...201728540315
listeriosis during pregnancy.listeriosis is a rare foodborne illness caused by listeria monocytogenes. it can be transmitted by consuming contaminated ready-to-eat food, long shelf-life products, deli meats, and soft cheeses. listeria has a predilection to affect immunocompromised patients, elderly people, pregnant women and neonates. in particular, pregnant women are at ~18 times greater risk of infection than general population due to specific pregnancy-related suppressed cell-mediated immunity and placental tropism of l. ...201728536811
production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays.in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ...201728536016
surfactin-loaded polyvinyl alcohol (pva) nanofibers alters adhesion of listeria monocytogenes to polystyrene.surfactin-loaded polyvinyl alcohol (pva) nanofibers were spun using gravity electrospinning. scanning electron microscopy (sem) images showed that nanofibers spun with surfactin are free from bead formation and uniform in diameter. the average nanofiber diameters were decreased (273±39nm, 259±39nm and 217±33nm) with increasing levels of surfactin (0.5, 1.0 and 1.5%, w/v) into pva (10%, w/v). the 10% (w/v) pva had average fiber diameter of 303±33nm. atomic force microscopy (afm) analysis showed t ...201728532029
getting "inside" type i ifns: type i ifns in intracellular bacterial infections.type i interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. discovered in the context of viral infections, type i ifns are now known to have myriad effects in infectious and autoimmune disease settings. type i ifn signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. as such, the repercussions ...201728529959
unusual case of spondylodiscitis due to listeria monocytogenes. 201628529845
listeria monocytogenes bacteraemia over an 11-year period: clinical and epidemiologic characteristics in the south area of madrid. 201728527675
effects of parsley extract powder as an alternative for the direct addition of sodium nitrite in the production of mortadella-type sausages - impact on microbiological, physicochemical and sensory aspects.increasing concern about chemical additives in processed meat has led to an increased market of uncured and alternatively cured meat products. however, the use of vegetable extracts or the exclusion of curing salt may increase the risk of greater bacterial growth and alteration of several physicochemical parameters. therefore, in this study mortadella-type sausages, manufactured with 1.07 (v3), 2.14 (v4) and 4.29 (v5) g parsley extract powder/kg sausage meat were produced. these sausage variants ...201728527368
water for agriculture: the convergence of sustainability and safety.agricultural water is a precious and limited resource. increasingly more water types and sources are being explored for use in irrigation within the united states and across the globe. as outlined in this chapter, the produce safety rule (psr) in the food safety and modernization act (fsma) provide irrigation water standards for application of water to fruits and vegetables consumed raw. these rules for production and use of water will continue to develop and be required as the world experiences ...201728527182
impact of acanthamoeba cysts on stress resistance of salmonella enterica serovar typhimurium, yersinia enterocolitica 4/o:3, listeria monocytogenes 1/2a, and escherichia coli o:26.the formation of robust resting cysts enables acanthamoeba to resist harsh environmental conditions. this study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cro ...201728526786
a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay.salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ...201728526589
investigation of optimum ohmic heating conditions for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in apple juice.control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. however, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. this study aims to investigate effective voltage gradients of an ohmic heat ...201728525985
outbreak-related disease burden associated with consumption of unpasteurized cow's milk and cheese, united states, 2009-2014.the growing popularity of unpasteurized milk in the united states raises public health concerns. we estimated outbreak-related illnesses and hospitalizations caused by the consumption of cow's milk and cheese contaminated with shiga toxin-producing escherichia coli, salmonella spp., listeria monocytogenes, and campylobacter spp. using a model relying on publicly available outbreak data. in the united states, outbreaks associated with dairy consumption cause, on average, 760 illnesses/year and 22 ...201728518026
a novel role of listeria monocytogenes membrane vesicles in inhibition of autophagy and cell death.bacterial membrane vesicle (mv) production has been mainly studied in gram-negative species. in this study, we show that listeria monocytogenes, a gram-positive pathogen that causes the food-borne illness listeriosis, produces mvs both in vitro and in vivo. we found that a major virulence factor, the pore-forming hemolysin listeriolysin o (llo), is tightly associated with the mvs, where it resides in an oxidized, inactive state. previous studies have shown that llo may induce cell death and auto ...201728516064
isolation and characterization of listeria phages for control of growth of listeria monocytogenes in milk.in this study, two listeria bacteriophages, lmp1 and lmp7, were isolated from chicken feces as a means of biocontrol of l. monocytogenes. both bacteriophages had a lytic effect on l. monocytogenes atcc 7644, 15313, 19114, and 19115. phages lmp1 and lmp7 were able to inhibit the growth of l. monocytogenes atcc 7644 and 19114 in tryptic soy broth at 10°c and 30°c. nevertheless, lmp1 was more effective than lmp7 at inhibiting l. monocytogenes atcc 19114. on the contrary, lmp7 was more effective tha ...201728515656
assessment of microbial and radioactive contaminations in korean cold duck meats and electron-beam application for quality improvement.animal-origin food products pose serious threat to public food safety due to high microbial loads. the microbial and radioactive contaminations in commercial cold duck meat products were evaluated. ten different lots of commercial samples (c1-c10) were classified based on type and smoking process. all samples were highly contaminated (< 4-7 log cfu/g) with total aerobic bacteria (tab), yeasts and molds (y&m), and 7 samples (c1-c7) were positive for coliforms. furthermore, three samples were cont ...201728515653
unusual listeria monocytogenes peritonitis in peritoneal dialysis patient with liver cirrhosis: a case report and review of literature.spontaneous listeria peritonitis is well described in liver failure, but is uncommon in peritoneal dialysis patients. atypical cases where peritonitis symptoms develop after systemic manifestations are rare and challenging for diagnostic. a 57-year-old peritoneal dialysis patient with history of ethylic cirrhosis was admitted after epileptic seizure. on admission, patient was soporous without signs of peritonitis and meningitis. patient's peritoneal effluent was clear, with normal leukocytes. cr ...201728509139
tetr-dependent gene regulation in intracellular listeria monocytogenes demonstrates the spatiotemporal surface distribution of acta.to enable specific and tightly controlled gene expression both in vitro and during the intracellular lifecycle of the pathogen listeria monocytogenes, a tetr-dependent genetic induction system was developed. highest concentration of cytoplasmic tetr and best repression of teto-controlled genes was obtained by tetr expression from the synthetic promoter pt17 . anhydrotetracycline (atc) as inducer permitted concentration-dependent, fine-tuned expression of genes under control of the teto operator ...201728508453
case-control study shows that neonatal pneumococcal meningitis cannot be distinguished from group b streptococcus cases.streptococcus pneumoniae (s. pneumoniae) is sometimes implicated in neonatal bacterial meningitis. this study described the demographic, clinical and biological features of neonatal s. pneumoniae meningitis and compared pneumococcal and group b streptococcal (gbs) neonatal meningitis.201728508419
limited colonization undermined by inadequate early immune responses defines the dynamics of decidual listeriosis.the bacterial pathogen listeria monocytogenes causes foodborne systemic disease in pregnant women, which can lead to preterm labor, stillbirth, or severe neonatal disease. colonization of the maternal decidua appears to be an initial step in the maternal component of the disease as well as bacterial transmission to the placenta and fetus. host-pathogen interactions in the decidua during this early stage of infection remain poorly understood. here, we assessed the dynamics of l. monocytogenes inf ...201728507070
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
preparation and properties of carbohydrate-based composite films incorporated with cuo nanoparticles.the present study aimed to develop the carbohydrate biopolymer based antimicrobial films for food packaging application. the nanocomposite films of various biopolymers and copper oxide nanoparticles (cuonps) were prepared by solvent casting method. the nanocomposite films were characterized using sem, ftir, xrd, and uv-vis spectroscopy. the thermal stability, uv barrier, water vapor permeability, and antibacterial activity of the composite films were also evaluated. the surface morphology of the ...201728504145
spontaneous bacterial peritonitis due to listeria monocytogenes: always to be remembered. 201728502590
identification of a major listeria monocytogenes outbreak clone linked to soft cheese in northern italy - 2009-2011.molecular subtyping and enhanced surveillance in lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. this cluster grouped 31 isolates that belonged to serotype 1/2a and sequence type 38 (st38) as defined by multilocus sequence typing (mlst).201728499357
mtorc1 regulates mannose-6-phosphate receptor transport and t-cell vulnerability to regulatory t cells by controlling kinesin kif13a.mannose-6-phosphate receptor (m6pr) that facilitates cellular uptake of m6p-bearing proteins, including serine-protease granzyme-b (gzm-b) has an important role in t-cell activation, migration and contraction. however, molecular mechanisms controlling m6pr expression in t cells remain poorly understood. here, we show that m6pr expression on t cells is distinctively controlled by two common γ-chain cytokines interleukin-2 (il-2) and il-7, and the differential m6pr expression is not caused by an a ...201728496990
genome sequences of listeria monocytogenes strains with resistance to arsenic.listeria monocytogenes frequently exhibits resistance to arsenic. we report here the draft genome sequences of eight genetically diverse arsenic-resistant l. monocytogenes strains from human listeriosis and food-associated environments. the availability of these genomes will help elucidate the role of heavy-metal resistance in the ecology of l. monocytogenes.201728495780
complete genome sequences of 12 isolates of listeria monocytogenes belonging to serotypes 1/2a, 1/2b, and 4b obtained from food products and food-processing environments in canada.listeria monocytogenes is the etiological agent for an often fatal foodborne illness known as listeriosis. here, we present the complete genome sequences of 12 l. monocytogenes isolates representing the three most common serotypes of this pathogen (1/2a, 1/2b, and 4b), collected in canada from different food products and environmental sources.201728495767
draft genome sequences of 25 listeria monocytogenes isolates associated with human clinical listeriosis in ireland.listeria monocytogenes is a gram-positive opportunistic pathogen that is the causative agent of listeriosis. here, we report the draft genome sequences of 25 l. monocytogenes strains isolated from patients with clinical listeriosis in the republic of ireland between 2013 and 2015.201728495762
microbial vertical transmission during human pregnancy.congenital infections with pathogens such as zika virus, toxoplasma gondii, listeria monocytogenes, treponema pallidium, parvovirus, hiv, varicella zoster virus, rubella, cytomegalovirus, and herpesviruses are a major cause of morbidity and mortality worldwide. despite the devastating impact of microbial infections on the developing fetus, relatively little is known about how pathogens associated with congenital disease breach the placental barrier to transit vertically during human pregnancy. i ...201728494237
listeria endocarditis: a diagnostic challenge.a 74-year-old hemodialysis patient with a history of an atrial septum defect closure, coronary bypass surgery, and a st. jude aortic prosthetic valve was diagnosed with pneumonia and volume overload. blood cultures were positive for listeria monocytogenes, and amoxicillin was given for 2 weeks. immediately after discontinuation of amoxicillin, fever relapsed. transthoracic and transesophageal echocardiography showed no sign of endocarditis. given the fever relapse and 3 positive minor duke crite ...201728491879
characteristics and outcome of spontaneous bacterial meningitis in patients with cancer compared to patients without cancer.in cancer patients, who are frequently immunocompromised, bacterial meningitis (bm) can be a severe complication, with a different presentation, etiology, and course, compared to patients without cancer. our objective is to compare the characteristics and outcomes of bm in patients with and without cancer. a single-center, prospective observational cohort study, conducted between 1982 and 2012, in a tertiary university hospital in barcelona (spain). the main outcome measure is in-hospital mortal ...201728489800
effects of sorbic acid-chitosan microcapsules as antimicrobial agent on the properties of ethylene vinyl alcohol copolymer film for food packaging.this paper discusses the possibility of using sorbic acid-chitosan microcapsules (s-mps) as an antibacterial component of active ethylene vinyl alcohol copolymer (evoh) film. s-mps with a diameter of approximately 1 to 4 μm showed a sorbic acid loading capacity of 46.5%. addition of s-mps (3%, w/w) increased the tensile strength, haze, oxygen, and water vapor barrier properties, as well as reduced the elongations at break and transmittance of s-mps-evoh (s-mp-evoh) film. antibacterial tests show ...201728488775
analysis of listeria using exogenous volatile organic compound metabolites and their detection by static headspace-multi-capillary column-gas chromatography-ion mobility spectrometry (shs-mcc-gc-ims).listeria monocytogenes is a gram-positive bacterium and an opportunistic food-borne pathogen which poses significant risk to the immune-compromised and pregnant due to the increased likelihood of acquiring infection and potential transmission of infection to the unborn child. conventional methods of analysis suffer from either long turn-around times or lack the ability to discriminate between listeria spp. reliably. this paper investigates an alternative method of detecting listeria spp. using t ...201728484808
etiology and laboratory abnormalities in bacterial meningitis in neonates and young infants.we conducted a retrospective review of electronic medical records of all cases of bacterial meningitis in neonates and young infants at our institution from 2004 to 2014. fifty-six cases were identified. the most common causative organism was group b streptococcus, followed by escherichia coli and then listeria monocytogenes. forty-four of the 56 patients in the study had abnormalities of the blood white blood cell (wbc) count. the most common wbc count abnormalities were leukopenia and elevatio ...201728484584
suppression of autoimmune demyelinating disease by preferential stimulation of cns-specific cd8 t cells using listeria-encoded neuroantigen.cd8 t-cells predominate in cns lesions of ms patients and display oligoclonal expansion. however, the role of myelin-specific cd8 t-cells in disease remains unclear, with studies showing protective and pathogenic roles in eae. we demonstrated a disease-suppressive function for cns-specific cd8 t-cells in a model where the antigen is exogenously administered in vivo and used for in vitro activation. to probe the nature of the cd8 response elicited by endogenously presented myelin antigens in vivo ...201728484224
label-free immuno-sensors for the fast detection of listeria in food.foodborne diseases are a major concern for both food industry and health organizations due to the economic costs and potential threats for human lives. for these reasons, specific regulations impose the research of pathogenic bacteria in food products. nevertheless, current methods, references and alternatives, take up to several days and require many handling steps. in order to improve pathogen detection in food, we developed an immune-sensor, based on surface plasmon resonance imaging (spri) a ...201728478556
two complementary approaches to quantify variability in heat resistance of spores of bacillus subtilis.realistic prediction of microbial inactivation in food requires quantitative information on variability introduced by the microorganisms. bacillus subtilis forms heat resistant spores and in this study the impact of strain variability on spore heat resistance was quantified using 20 strains. in addition, experimental variability was quantified by using technical replicates per heat treatment experiment, and reproduction variability was quantified by using two biologically independent spore crops ...201728478320
a novel typing method for listeria monocytogenes using high-resolution melting analysis (hrma) of tandem repeat regions.listeria monocytogenes, which is responsible for causing food poisoning known as listeriosis, infects humans and animals. widely distributed in the environment, this bacterium is known to contaminate food products after being transmitted to factories via raw materials. to minimize the contamination of products by food pathogens, it is critical to identify and eliminate factory entry routes and pathways for the causative bacteria. high resolution melting analysis (hrma) is a method that takes adv ...201728475927
inll from listeria monocytogenes is involved in biofilm formation and adhesion to mucin.the bacterial etiological agent of listeriosis, listeria monocytogenes, is an opportunistic intracellular foodborne pathogen. the infection cycle of l. monocytogenes is well-characterized and involves several key virulence factors, including internalins a and b. while 35 genes encoding internalins have been identified in l. monocytogenes, less than half of them have been characterized as yet. focusing on lmo2026, it was shown this gene encodes a class i internalin, inll, exhibiting domains poten ...201728473809
isolation and characterization of listeria monocytogenes from the black-headed gull feces in kunming, china.listeria monocytogenes is among the most important foodborne pathogens, which can be isolated from bird feces. the aim of this study was to examine the prevalence, genotypes, virulence factors and drug-resistance characteristics of l. monocytogenes isolated from the black-headed gull in kunming, china. serotypes and virulence genes were identified by polymerase chain reaction. pulsed-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst) methods were used to describe the genetic ...201728473260
listeria monocytogenes meningitis: pcr multiplex diagnosis. 201728473173
comparative genomics of human and non-human listeria monocytogenes sequence type 121 strains.the food-borne pathogen listeria (l.) monocytogenes is able to survive for months and even years in food production environments. strains belonging to sequence type (st)121 are particularly found to be abundant and to persist in food and food production environments. to elucidate genetic determinants characteristic for l. monocytogenes st121, we sequenced the genomes of 14 st121 strains and compared them with currently available l. monocytogenes st121 genomes. in total, we analyzed 70 st121 geno ...201728472116
comparative experimental infection of listeria monocytogenes and listeria ivanovii in bovine trophoblasts.listeria monocytogenes is a gram-positive, facultative intracellular and invasive bacterium that has tropism to the placenta, and causes fetal morbidity and mortality in several mammalian species. while infection with l. monocytogenes and l. ivanovii are known as important causes of abortion and reproductive failure in cattle, the pathogenesis of maternal-fetal listeriosis in this species is poorly known. this study used the bovine chorioallantoic membrane explant model to investigate the kineti ...201728467447
potential of cinnamon oil emulsions as alternative washing solutions of carrots.the objective of this study was to evaluate the potential of cinnamon oil emulsions as alternative washing solutions to improve the microbial safety of carrots. whey protein concentrate (wpc), gum arabic (ga), lecithin, and their combinations were used to prepare cinnamon oil emulsions. the emulsions were characterized for their hydrodynamic diameter (dh) during 7 days of storage and their antimicrobial activity against cocktails of salmonella enterica , escherichia coli o157:h7, and listeria mo ...201728467186
synergistic effect of plantaricin bm-1 combined with physicochemical treatments on the control of listeria monocytogenes in cooked ham.plantaricin bm-1, a typical iia bacteriocin, is produced by lactobacillus plantarum bm-1, which can be isolated from a traditionally fermented chinese meat product. this bacteriocin exhibits perfect thermal stability and broad inhibitory activity against certain foodborne pathogens. in this study, we investigated the effect of plantaricin bm-1 combined with physicochemical treatments (sodium nitrite, heat treatment, ultrahigh pressure technology) on the control of listeria monocytogenes populati ...201728467183
lentinan from shiitake selectively attenuates aim2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production.lentinan extracted from shiitake (lentinula edodes) is a β-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. in this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloid cells. mouse bone marrow-derived macrophages were treated with lentinan with/without inflammasome triggers, and maturation of interleukin (il)-1β, il-18, or caspase-1 was measured as a readout of inflammasom ...201728465544
a clade of listeria monocytogenes serotype 4b variant strains linked to recent listeriosis outbreaks associated with produce from a defined geographic region in the us.four listeriosis incidences/outbreaks, spanning 19 months, have been linked to listeria monocytogenes serotype 4b variant (4bv) strains. three of these incidents can be linked to a defined geographical region, while the fourth is likely to be linked. in this study, whole genome sequencing (wgs) of strains from these incidents was used for genomic comparisons using two approached. the first was jspecies tetramer, which analyzed tetranucleotide frequency to assess relatedness. the second, the cfsa ...201728464038
formulation and shelf-life of fish burgers served to preschool children.consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. in order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (map). fish species used for trail were trachurus tr ...201728462206
analysis of process factors of dry fermented salami to control listeria monocytogenes.challenge tests are a clear opportunity for manufacturers interested in the evaluation of their management system with the aim to reduce the spread of foodborne pathogens. this is a main concern especially in ready-to-eat food in relation to the risk associated with listeria monocytogenes. for small and medium-scale food industry the manufacturing practices and products formulation are characterised by a wider variability and poor repeatability. the use of ad hoc challenge test and the compariso ...201728462203
host serine/threonine kinases mtor and protein kinase c-α promote inlb-mediated entry of listeria monocytogenes.the bacterial pathogen listeria monocytogenes causes foodborne illnesses resulting in gastroenteritis, meningitis, or abortion. listeria induces its internalization into some human cells through interaction of the bacterial surface protein inlb with the host receptor tyrosine kinase met. inlb-dependent entry requires localized polymerization of the host actin cytoskeleton. the signal transduction pathways that act downstream of met to regulate actin filament assembly or other processes during li ...201728461391
thermal inactivation kinetics of surface contaminating listeria monocytogenes on vacuum-packaged agar surface and ready-to-eat sliced ham and sausage.the aim of this work was to study thermal inactivation kinetics of listeria monocytogenes on vacuum-packaged food surfaces. the kinetics were first determined on model agar systems (bhi agar plates), mimicking cooked meat products, which have the same characteristics (ph, sodium chloride (nacl) or sodium lactate (nal) content and thickness) as the cooked meat products. then, in order to validate how well the thermal inactivation on the model agar system simulated inactivation on real products, i ...201628460987
influence of processing parameters and stress adaptation on the inactivation of listeria monocytogenes by non-thermal atmospheric plasma (ntap).this study evaluated the effectiveness of non-thermal atmospheric plasma (ntap) treatments against listeria. firstly, the impact of gas composition and flow rate on l. monocytogenes and l. innocua (used as a surrogate) inactivation by ntap was monitored. secondly, the influence of stress adaptation (growth under suboptimal conditions, using a wide range of temperatures and media acidified up to ph5.5 with citric, lactic, malic or hydrochloric acid, or short-term exposure to acid, cold or thermal ...201628460960
molecular characterization of silver-stearate nanoparticles (agstnps): a hydrophobic and antimicrobial material against foodborne pathogens.in this study, silver-stearate nanoparticles (agstnps) have been produced from silver nitrate solutions by replacing the nitrate by stearate ions and then reducing by thermal treatment. agstnps were characterized by particle size, zeta-potential, atr-ftir, contact angle and sem (scanning electron microscopy) analyses. the mean particle size and zeta potential of agstnps were determined to be 69.22±7.30nm and +8.02±0.88mv. atr-ftir analysis showed characteristic ir bands of stearate, revealing th ...201528455024
the challenge of challenge testing to monitor listeria monocytogenes growth on ready-to-eat foods in europe by following the european commission (2014) technical guidance document.european regulation (ec) no. 2073/2005 lays down the microbiological criteria for certain microorganisms in foods and the implementing rules to be complied with by food business operators (fbos) in europe when implementing general and specific hygiene measures. in relation to listeria monocytogenes, this regulation covers primarily ready-to-eat (rte) food products, and requires different microbiological criteria depending on the ability of the food product to support growth of l. monocytogenes. ...201528454952
modeling the protective effect of aw and fat content on the high pressure resistance of listeria monocytogenes in dry-cured ham.high pressure processing (hpp) is a promising food preservation technology as an alternative to thermal processing for microbial inactivation. the technological parameters, the type of microorganism, and the food composition can greatly affect the microbicidal potential of hpp against spoilage and pathogenic microorganisms. presently, the number of available models quantifying the influence of food characteristics on the pathogen inactivation is scarce. the aim of this study was to model the ina ...201528454947
genetic and environmental factors influence listeria monocytogenes nisin resistance.listeria monocytogenes nisin resistance increases when first exposed to nacl and other stresses, such as low ph. in addition to environmental stressors, specific genomic elements can confer nisin resistance, such as the stress survival islet (ssi-1). as ssi-1 is variably present among l. monocytogenes strains, we wanted to determine if ssi-1 was associated with salt-induced nisin resistance.201728452154
insights into clpxp proteolysis: heterooligomerization and partial deactivation enhance chaperone affinity and substrate turnover in listeria monocytogenes.caseinolytic proteases (clpp) are important for recognition and controlled degradation of damaged proteins. while the majority of bacterial organisms utilize only a single clpp, listeria monocytogenes expresses two isoforms (lmclpp1 and lmclpp2). lmclpps assemble into either a lmclpp2 homocomplex or a lmclpp1/2 heterooligomeric complex. the heterocomplex in association with the chaperone clpx, exhibits a boost in proteolytic activity for unknown reasons. here, we use a combined chemical and bioc ...201728451288
structural basis of crispr-spycas9 inhibition by an anti-crispr protein.crispr-cas9 systems are bacterial adaptive immune systems that defend against infection by phages. through the rna-guided endonuclease activity of cas9 they degrade double-stranded dna with a protospacer adjacent motif (pam) and sequences complementary to the guide rna. recently, two anti-crispr proteins (acriia2 and acriia4 from listeria monocytogenes prophages) were identified, both of which inhibit streptococcus pyogenes cas9 (spycas9) and l. monocytogenes cas9 activity in bacteria and human ...201728448066
prevalence, antimicrobial susceptibility and multiplex pcr-serotyping of listeria monocytogenes isolated from humans, foods and livestock in iran.listeria monocytogenes is a foodborne pathogen causing listeriosis, which potentially affects all individuals, especially pregnant women and immunocompromised persons. the present study investigated the prevalence, antimicrobial susceptibility and serotypes distribution of the isolated l. monocytogenes from iran. twenty two (4.97%) of 442 human, food and livestock samples were found to be positive for l. monocytogenes. l. monocytogenes was identified in 8.8% of 125 human samples, 2.99% of 267 fo ...201728445701
survey for listeria monocytogenes in and on ready-to-eat foods from retail establishments in the united states (2010 through 2013): assessing potential changes of pathogen prevalence and levels in a decade.a multiyear interagency listeria monocytogenes market basket survey was undertaken for selected refrigerated ready-to-eat foods purchased at retail in four foodnet sites in the united states. food samples from 16 food categories in six broad groups (seafood, produce, dairy, meat, eggs, and combination foods) were collected weekly at large national chain supermarkets and independent grocery stores in california, maryland, connecticut, and georgia for 100 weeks between december 2010 and march 2013 ...201728437165
short communication: microbiological quality of raw cow milk and its association with herd management practices in northern china.contamination of raw milk with bacterial pathogens is potentially hazardous to human health. the aim of this study was to evaluate the total bacteria count (tbc) and presence of pathogens in raw milk in northern china along with the associated herd management practices. a total of 160 raw milk samples were collected from 80 dairy herds in northern china. all raw milk samples were analyzed for tbc and pathogens by culturing. the results showed that the number of raw milk samples with tbc <2 × 10( ...201728434737
quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections.lysosomes play vital roles in both innate and adaptive immunity. it is widely accepted that lysosomes do not function exclusively as a digestive organelle. it is also involved in the process of immune cells against pathogens. however, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lysosome is another obstacle that needs to be resolved. here, we performed a proteomic study on lysosome ...201728433889
relating physicochemical and microbiological safety indicators during processing of linguiça, a portuguese traditional dry-fermented sausage.linguiça is a portuguese traditional fermented sausage whose microbiological quality and safety can be highly variable. in order to elucidate risk factors and the particularities of the manufacturing technology that explain the between-batch variability in total viable counts (tvc), enterobacteriaceae, staphylococcus aureus and listeria monocytogenes in the product; microbiological and physicochemical characterisation of linguiça at five stages of production (i.e., raw pork meat, mixed with ingr ...201528433317
analysis of the listeria monocytogenes population structure among isolates from 1931 to 2015 in australia.listeriosis remains among the most important bacterial illnesses, with a high associated mortality rate. efforts to control listeriosis require detailed knowledge of the epidemiology of the disease itself, and its etiological bacterium, listeria monocytogenes. in this study we provide an in-depth analysis of the epidemiology of 224 l. monocytogenes isolates from australian clinical and non-clinical sources. non-human sources included meat, dairy, seafood, fruit, and vegetables, along with animal ...201728428781
draft genome sequences of three listeria monocytogenes isolates from foods in china.listeria monocytogenes is a foodborne pathogen of global concern because of the high mortality rate among patients. the draft genome sequences of three l. monocytogenes strains isolated from foods are reported here. the availability of these genomes should provide useful information on the genomic diversity of l. monocytogenes isolated from foods in china.201728428307
unique case report of a chromomycosis and listeria in soft tissue and cerebellar abscesses after kidney transplantation.chromomycosis is a rare mycotic infection encountered in tropical and subtropical regions. the disease presents as a slowly-evolving nodule that can become infected with bacteria. here, we describe a unique association of abscesses caused by a chromomycosis and listeria monocytogenes in a kidney transplant recipient, and didactically expose how the appropriate diagnosis was reached.201728427354
a comparative study of natural antimicrobial delivery systems for microbial safety and quality of fresh-cut lettuce.nanoencapsulation can provide a means to effectively deliver antimicrobial compounds and enhance the safety of fresh produce. however, to date there are no studies which directly compares how different nanoencapsulation systems affect fresh produce safety and quality. this study compared the effects on quality and safety of fresh-cut lettuce treated with free and nanoencapsulated natural antimicrobial, cinnamon bark extract (cbe). a challenge study compared antimicrobial efficacy of 3 different ...201728425569
listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes.we analyzed the pathogenicity of various serotypes of listeria monocytogenes using a balb/c mouse intravenous injection model. the survival rates of mice inoculated with strains ns1/2b (serotype 1/2b), ns3b (serotype 3b) and ns 4b (serotype 4b) were 60, 63.6 and 63.6%, respectively. although the survival rates were similar, the bacterial growth in the liver of ns3b-infected mice was 144.5-fold higher than that in the liver of ns4b-infected mice. histopathological analyses suggest that the ns4b s ...201728420823
listeria monocytogenes meningitis in the netherlands, 1985-2014: a nationwide surveillance study.listeria monocytogenes can cause sepsis and meningitis. we report national surveillance data on l. monocytogenes meningitis in the netherlands, describing incidence changes, genetic epidemiology and fatality rate.201728419853
host pore-forming protein complex neutralizes the acidification of endocytic organelles to counteract intracellular pathogens.many intracellular pathogens invade cells via endocytic organelles and have adapted to the drop in ph along the endocytic pathway. however, the strategy by which the host cell counteracts this pathogen adaptation remains unclear. βγ-cat is an aerolysin-like pore-forming protein and trefoil factor complex in the frog bombina maxima. we report here that βγ-cat, as a host-secreted factor with an intrinsic channel-forming property, is the first example of a molecule that actively neutralizes the aci ...201728419297
diversity of endophytic bacteria in a fern species dryopteris uniformis (makino) makino and evaluation of their antibacterial potential against five foodborne pathogenic bacteria.the fern plant dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. this study was conducted to explore the endophytic bacterial diversity associated with d. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (fpb). among 51 isolated endophytic bacteria (eb), 26 eb were selected based on their morphological characteristics and identified by 16s rrna gene analysis. the distribution of eb was diverse i ...201728418717
application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review.electrolysed oxidising water (e.o. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. at neutral ph this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. e.o. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. the efficacy of e.o. water against pathogenic ba ...201728416883
postchill antimicrobial treatments to control salmonella, listeria, and campylobacter contamination on chicken skin used in ground chicken.ground poultry products are frequently contaminated with foodborne pathogens. with the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. a large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. in this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of per ...201728414258
capability of exopolysaccharide-producing lactobacillus paraplantarum bgcg11 and its non-producing isogenic strain nb1, to counteract the effect of enteropathogens upon the epithelial cell line ht29-mtx.the putative protective role of the exopolysaccharide (eps)-producing lactobacillus paraplantarum bgcg11, and its non-eps-producing isogenic strain nb1, was tested upon ht29-mtx monolayers challenged with seven opportunistic pathogens. the probiotic strain lactobacillus rhamnosus lmg18243 (gg) was used as a reference bacterium. tested lactobacilli were able to efficiently reduce the attachment to ht29-mtx of most pathogens. lb. paraplantarum nb1 and lb. rhamnosus gg were more efficient reducing ...201528411984
comparative diagnostic efficacy of recombinant llo and pi-plc-based elisas for detection of listeriosis in animals.the present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin o (rllo) and phosphatidyl-inositol phospholipase c (rpi-plc). indirect elisa with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. isolation of listeria was attempted from the blood samples using usda-fsis method. on screening o ...201728411150
antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide.brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ...201728407186
antibacterial effects of lactobacillus isolates of curd and human milk origin against food-borne and human pathogens.this study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific pcr and 16s rrna-based sequencing. these strains belonged to five species, lactobacillus casei, l. delbrueckii, l. fermentum, l. plantarum, and l. pentosus. antibacterial activities of cell-free supernatants (cfss) of all the lactobacillus isolates were estim ...201728401466
screening of the two-component-system histidine kinases of listeria monocytogenes egd-e. lias is needed for growth under heat, acid, alkali, osmotic, ethanol and oxidative stresses.to study the role of each two-component system (tcs) histidine kinase (hk) in stress tolerance of listeria monocytogenes egd-e, we monitored the growth of individual hk deletion mutant strains under heat (42.5 °c), acid (ph 5.6), alkali (ph 9.4), osmotic (6% nacl), ethanol (3.5 vol%), and oxidative (5 mm h2o2) stresses. the growth of δlias (δlmo1021) strain was impaired under each stress, with the most notable decrease under heat and osmotic stresses. the δvirs (δlmo1741) strain showed nearly co ...201728400017
pathological findings in the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in northern germany.anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. the present study provides a detailed characterisation of the health status of the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereu ...201728399176
pregnancy-related listeriosis.about one in seven cases of listeriosis occurs in pregnant women, and, although listeriosis is rare, it is the third leading cause of death from food-borne infections. pregnancy-related listeriosis increases the risk for fetal and neonatal mortality by approximately 21%. during pregnancy, infections are more likely to occur in the third trimester (66%) than the first trimester (3%). however, fetal and neonatal adverse effects are less common as gestational age increases or with older gestational ...201728398675
the burden and epidemiology of community-acquired central nervous system infections: a multinational study.risk assessment of central nervous system (cns) infection patients is of key importance in predicting likely pathogens. however, data are lacking on the epidemiology globally. we performed a multicenter study to understand the burden of community-acquired cns (ca-cns) infections between 2012 and 2014. a total of 2583 patients with ca-cns infections were included from 37 referral centers in 20 countries. of these, 477 (18.5%) patients survived with sequelae and 227 (8.8%) died, and 1879 (72.7%) p ...201728397100
metabolic genetic screens reveal multidimensional regulation of virulence gene expression in listeria monocytogenes and an aminopeptidase that is critical for prfa protein activation.listeria monocytogenes is an environmental saprophyte and intracellular bacterial pathogen. upon invading mammalian cells, the bacterium senses abrupt changes in its metabolic environment, which are rapidly transduced to regulation of virulence gene expression. to explore the relationship between l. monocytogenes metabolism and virulence, we monitored virulence gene expression dynamics across a library of genetic mutants grown under two metabolic conditions known to activate the virulent state: ...201728396325
listeria monocytogenes meningitis complicating rotavirus gastroenteritis in an immunocompetent child.listeria monocytogenes only occasionally causes bacterial meningitis in immunocompetent children. we report a case of l. monocytogenes meningitis associated with rotavirus gastroenteritis. the patient was a previously healthy 20-month-old girl who was admitted because of sustained fever and lethargy after suffering from gastroenteritis for 6 days. the patient's peripheral white blood cell count was 18,600/µl and the c-reactive protein level was 2.44 mg/dl. a stool sample tested positive for rota ...201728392538
a study on prevalence of microbial contamination on the surface of raw salad vegetables.the present work evaluates the microbiological quality of raw salad vegetables (rsv) consumed in dhanbad city, india. a total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. e. coli o157:h7, listeria monocytogenes and salmonella sp. were detected by real-time polymerase chain reaction (qpcr) subsequent to isolation. results showed that al ...201728391475
finding novel antibiotic substances from medicinal plants - antimicrobial properties of nigella sativa directed against multidrug-resistant bacteria.the progressive rise in multidrug-resistant (mdr) bacterial strains poses serious problems in the treatment of infectious diseases. while the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. this rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. the medicinal herb nigella sativa and its derivatives constitute promising candidates. ...201728386474
comparative phenotypic and genotypic analysis of swiss and finnish listeria monocytogenes isolates with respect to benzalkonium chloride resistance.reduced susceptibility of listeria monocytogenes to benzalkonium chloride (bc), a quaternary ammonium compound widely used in food processing and hospital environments, is a growing public health and food safety concern. the minimal inhibitory concentration of bc on 392 l. monocytogenes strains from switzerland (ch) and finland (fin) was determined. within this strain collection, benzalkonium chloride resistance was observed in 12.3% (24/195) of swiss and 10.6% (21/197) of finnish strains. in bo ...201728386248
bacteriocins for control of listeria spp. in food.foodborne outbreaks of listeriosis caused by listeria monocytogenes have contributed to public consciousness about bacterial pathogens involved with foodborne disease. major concerns with l. monocytogenes are its high mortality rate, wide distribution on raw products, growth at low temperatures, and its ability to establish itself in various food-processing environments. these concerns have prompted the examination of novel approaches, including the use of antimicrobial peptides, or bacteriocins ...199628384026
local outbreak of listeria monocytogenes serotype 4b sequence type 6 due to contaminated meat pâté.in january and february 2016, five cases of confirmed and two cases of probable infection due to listeria monocytogenes serotype 4b, sequence type (st) 6 belonging to a single pulsed-field gel electrophoresis pulsotype pattern were registered in a region of southern switzerland. l. monocytogenes was detected in blood samples (four cases) and pleural fluid (one case). furthermore, l. monocytogenes 4b st6 was detected in a stool sample of an asymptomatic person exposed to a common food. forthwith, ...201728379731
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
listeriolysin s is a streptolysin s-like virulence factor that targets exclusively prokaryotic cells in vivo.streptolysin s (sls)-like virulence factors from clinically relevant gram-positive pathogens have been proposed to behave as potent cytotoxins, playing key roles in tissue infection. listeriolysin s (lls) is an sls-like hemolysin/bacteriocin present among listeria monocytogenes strains responsible for human listeriosis outbreaks. as lls cytotoxic activity has been associated with virulence, we investigated the lls-specific contribution to host tissue infection. surprisingly, we first show that l ...201728377528
listeria monocytogenes infection associated with alemtuzumab - - a case for better preventive strategies.the mortality of septicaemia, meningitis and encephalitis caused by listeria monocytogenes is 20-40%. twenty-one cases of invasive listeriosis associated with alemtuzumab, including at least 16 in patients with multiple sclerosis, have been published or reported to the world health organization case safety reports database. three cases were fatal, including at least one patient treated for multiple sclerosis in 2016.201728376817
whey protein isolate/cellulose nanofibre/tio2 nanoparticle/rosemary essential oil nanocomposite film: its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration.the use of biodegradable nanocomposite films in active packaging is of great importance since they can have a controlled release of antimicrobial compounds. this study was conducted to evaluate the efficacy of whey protein isolate (wpi)/cellulose nanofibre (cnf) nanocomposite films containing 1.0% (w/w) titanium dioxide (tio2) and 2.0% (w/v) rosemary essential oil (reo) in preserving the microbial and sensory quality of lamb meat during the storage at 4±1°c. initially, the best concentration of ...201728376399
chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition.essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ...201628375110
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