Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| five-year summary of in vitro activity and resistance mechanisms of linezolid against clinically important gram-positive cocci in the united states from the leader surveillance program (2011 to 2015). | this report describes linezolid susceptibility testing results for 6,741 gram-positive pathogens from 60 u.s. sites collected during 2015 for the leader program. in addition, the report summarizes linezolid in vitro activity, resistance mechanisms, and molecular typing obtained for 2011 to 2015. during 2015, linezolid showed potent activity in testing against staphylococcus aureus, inhibiting >99.9% of 3,031 isolates at ≤2 µg/ml. similarly, linezolid showed coverage against 99.2% of coagulase-ne ... | 2017 | 28483950 |
| association of metal homeostasis and (p)ppgpp regulation in the pathophysiology of enterococcus faecalis. | in enterococcus faecalis, the regulatory nucleotides pppgpp and ppgpp, collectively, (p)ppgpp, are required for growth in blood, survival within macrophages, and virulence. however, a clear understanding of how (p)ppgpp promotes virulence in e. faecalis and other bacterial pathogens is still lacking. in the host, the essential transition metals iron (fe) and manganese (mn) are not readily available to invading pathogens because of a host-driven process called nutritional immunity. considering it ... | 2017 | 28483855 |
| cytoprotective effect of the enzyme-mediated polygallic acid on fibroblast cells under exposure of uv-irradiation. | the poly(gallic acid), produced by laccase-mediated oxidation of gallic acid in aqueous media (ph5.5) to attain a novel material with well-defined molecular structure and high water solubility (500mg/ml at 25°c), has been investigated to understand its potential biological activities. in this regard, a biomedical approach based on cytoprotective effect on human fibroblast cells exposed to uv-irradiation in the presence of the polymer has been demonstrated. the results also shows that 200μg/ml of ... | 2017 | 28482545 |
| use of photo-fenton's reaction by 400-nm led light for endodontic disinfection: a preliminary in vitro study on enterococcus faecalis. | one of the biggest challenges in endodontics is the complete disinfection of root canals. in addition to mechanical preparation, the technique traditionally also involves channel disinfection with other agents such as sodium hypochlorite, hydrogen peroxide, chlorhexidine, or a combination of these. some bacterial species are particularly resistant to eradication. using enterococcus faecalis in this preliminary study, we tested the bactericidal effectiveness of the fenton reaction and the photo-f ... | 2017 | 28482224 |
| daptomycin: new insights into an antibiotic of last resort. | 2017 | 28481142 | |
| studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds. | natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars. | 2017 | 28480436 |
| does the removal of cementum facilitate bacterial penetration into dentinal tubules in vitro? | the importance of an intact layer of cementum on the root surface in preventing bacterial penetration into radicular dentin has not been sufficiently investigated. the aim of this in vitro study was to determine the effect of the absence of cementum from the root surface and the length of the infection period (2 or 4 weeks) on the maximum depth of bacterial penetration and the percentage of sectors lined with bacteria. | 2017 | 28476468 |
| urinary tract infections in postmenopausal women with type 2 diabetes: clinical correlates and quinolone susceptibility. | introduction diabetes mellitus and the postmenopausal period are associated with increased risk of urinary tract infections (utis) in women. however, data on utis in postmenopausal diabetic women are scarce. objectives the aim of this study was to determine the prevalence of utis in postmenopausal women with type 2 diabetes mellitus, identify the potential risk factors, describe the causative pathogens, and assess their susceptibility to quinolones. patients and methods patients were interviewe ... | 2017 | 28475170 |
| a short artificial antimicrobial peptide shows potential to prevent or treat bone infections. | infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. antimicrobial peptides (amps) are naturally occurring peptides and promising candidates for treatment of joint infections. this study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficac ... | 2017 | 28473710 |
| chicken embryo lethality assay for determining the lethal dose and virulence of enterococcus faecalis. | enterococcus faecalis is the major pathogen found in field cases of amyloid arthropathy in chickens. given the need for a better understanding of the virulence mechanisms of the causative strains, the embryo lethality assay (ela) is proposed in the present study as a model to evaluate the virulence of e. faecalis strains, specifically the pathogenic avian strain k923/96, which was previously related with amyloid arthropathy. hence, 0.2 ml of five doses of the cited strain (from 2.5 to 2500 colon ... | 2017 | 28470098 |
| validity of somatic cell count as indicator of pathogen-specific intramammary infections. | the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ... | 2017 | 28470079 |
| evaluation of antibacterial efficacy of photodynamic therapy vs. 2.5% naocl against e. faecalis-infected root canals using real-time pcr technique. | bacteria like e. faecalis can produce intra- and extra-radicular biofilms. theoretically, the adjustable penetration ability of lasers enables better access to root canal system. therefore the aim of the present study was to compare the ability of photoactivated laser and 2.5% naocl irrigation solution to eliminate e. faecalis from the root canals by real-time pcr technique. | 2017 | 28469819 |
| role of surface energy and nano-roughness in the removal efficiency of bacterial contamination by nonwoven wipes from frequently touched surfaces. | healthcare associated infections (hcais) are responsible for substantial patient morbidity, mortality and economic cost. infection control strategies for reducing rates of transmission include the use of nonwoven wipes to remove pathogenic bacteria from frequently touched surfaces. wiping is a dynamic process that involves physicochemical mechanisms to detach and transfer bacteria to fibre surfaces within the wipe. the purpose of this study was to determine the extent to which systematic changes ... | 2017 | 28469734 |
| molecular mechanism of quorum-sensing in enterococcus faecalis: its role in virulence and therapeutic approaches. | quorum-sensing systems control major virulence determinants in enterococcusfaecalis, which causes nosocomial infections. the e. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. in addition, the e. faecalis fsr regulator system controls the expression of gelatinase, serine protease, and enterocin o16. the cytolysin and fsr virulence factor systems are linked to enterococcal diseases that affect the hea ... | 2017 | 28467378 |
| self assembled snowball-like hybrid nanostructures comprising viburnum opulus l. extract and metal ions for antimicrobial and catalytic applications. | herein, we report the production of novel organic-inorganic nanobio-catalytic and antimicrobial agents called "nanosnowball" (nsbs) with a rational design and elucidate the increase in the catalytic and antimicrobial activities. the nsbs resemble to guelder rose were formed of the extract of viburnum opulus (vo) as the organic component and copper (ii) ions (cu(2+)) as the inorganic component. the effects of the vo extract and cu(2+) concentrations on the morphology of nsbs were systematically e ... | 2017 | 28465062 |
| total staphylococci as performance surrogate for greywater treatment. | faecal indicator bacteria (fib) are commonly used as water quality indicators; implying faecal contamination and therefore the potential presence of pathogenic enteric bacteria, viruses, and protozoa. hence in wastewater treatment, the most commonly used treatment process measures (surrogates) are total coliforms, faecal coliforms, escherichia coli (e. coli), and enterococci. however, greywater potentially contains skin pathogens unrelated to faecal load, and e. coli and other fib may grow withi ... | 2017 | 28462431 |
| applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) can be used to identify pathogens in blood culture samples. however, sample pretreatment is needed for direct identification of microbes in blood culture bottles. conventional protocols are complex and time-consuming. therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. using representative ba ... | 2017 | 28461023 |
| bacterial diversity of the gastric content of preterm infants during their first month of life at the hospital. | studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. the aim of this work is to describe the bacterial communities inhabiting the gastric content (gc) of preterm neonates. for that purpose, gc samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. samples were analyzed by using both culture-dependent and -independent techniques. the former allowed the isolatio ... | 2017 | 28459051 |
| transcriptomics analysis reveals putative genes involved in biofilm formation and biofilm-associated drug resistance of enterococcus faecalis. | enterococcus faecalis is a gram-positive bacterium associated with endodontic infections and is capable of forming biofilms that can confer drug resistance to the bacterium, resulting in treatment failure. current knowledge on e. faecalis drug resistance is of a limited and conflicting nature. the present study examined the genetic basis of e. faecalis biofilm formation and drug resistance using a rna sequencing (rna-seq)-based transcriptome approach. | 2017 | 28457636 |
| high-purity nisin alone or in combination with sodium hypochlorite is effective against planktonic and biofilm populations of enterococcus faecalis. | nisin, a broad-spectrum bacteriocin, has recently been highlighted for its biomedical applications. to date, no studies have examined the antimicrobial and antibiofilm properties of high-purity (>95%) nisin (nisin zp) on enterococcus faecalis and biofilms formed by this species. we hypothesize that nisin can inhibit e. faecalis and reduce biofilm biomass, and combinations of nisin and sodium hypochlorite (naocl) will enhance the antibiofilm properties against e. faecalis biofilms. | 2017 | 28457635 |
| enzymes required for maltodextrin catabolism in enterococcus faecalis exhibit novel activities. | maltose and maltodextrins are formed during the degradation of starch or glycogen. maltodextrins are composed of a mixture of maltooligosaccharides formed by α-1,4- but also some α-1,6-linked glucosyl residues. the α-1,6-linked glucosyl residues are derived from branching points in the polysaccharides. in enterococcus faecalis, maltotriose is mainly transported and phosphorylated by a phosphoenolpyruvate:carbohydrate phosphotransferase system. the formed maltotriose-6″-phosphate is intracellular ... | 2017 | 28455338 |
| response surface method optimization of a novel hypericin formulation in p123 micelles for colorectal cancer and antimicrobial photodynamic therapy. | the photodynamic properties of hypericin (hyp) may be used as an alternative treatment for malignancies of the lower gastrointestinal tract and for the prevention of surgical-site infection; however, its use in photodynamic therapy has been limited because of its poor hydrosolubility. therefore, in order to improve its water solubility and its photodynamic effect, hyp was encapsulated in pluronic p123 (p123) and the photodynamic effects against intestinal and epidermal bacteria and against two l ... | 2017 | 28454049 |
| recurrence of deep surgical site infection in cerebral palsy after spinal fusion is rare. | retrospective review of prospective registry. | 2017 | 28449964 |
| growth inhibition and antibiofilm potential of ag nanoparticles coated with lectin, an arthropod immune molecule. | lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. here, lectin was purified from the haemolymph of blue swimmer crab portunus pelagicus, using mannose-coupled sepharose cl-4b affinity column chromatography. in sds-page analysis, lectin showed a molecular mass of approximately 155kda. the synthesis of lectin-coated silver nanoparticles (lectin-coated agnps) was confirmed by uv-vis spectroscopy, xrd, fti ... | 2017 | 28441606 |
| synthesis and bioactivity evaluation of novel 2-salicyloylbenzofurans as antibacterial agents. | in order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three gram-(+) strains (methicillin-sensitive staphylococcus aureus (mssa) atcc 29213, methicillin-resistant staphylococcus aureus (mrsa) atcc 43300, and streptococcus faecalis (s. faecalis) atcc 29212) and one gram-(-) strain (escherichia coli (e. coli) atcc 25922). the 2-salicyloylbenzofuran heterocycles were generated b ... | 2017 | 28441350 |
| substance p enhances lactic acid and tyramine production in enterococcus faecalis v583 and promotes its cytotoxic effect on intestinal caco-2/tc7 cells. | enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. this bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. the aim of this study was to evaluate the effect on e. faecalis, of substance p (sp), an antimicrobial peptide th ... | 2017 | 28439299 |
| comparison of linezolid and vancomycin lock solutions with and without heparin against biofilm-producing bacteria. | the activity of linezolid and vancomycin lock solutions against biofilm-producing strains of staphylococcus aureus, s. epidermidis, and enterococcus faecalis was studied. | 2017 | 28438824 |
| comparative evaluation of antimicrobial efficacy of an alternative natural agent for disinfection of toothbrushes. | the aim of this study was to assess the antimicrobial potency of aqueous extract of psidium guajava leaves in two different concentrations as a toothbrush disinfectant against three oral bacterial species. | 2017 | 28435376 |
| comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens. | the objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (tap) and a proton pump inhibitor (ppi) (omeprazole) in combination with calcium hydroxide (ch) against enterococcus faecalis and candida albicans. | 2017 | 28435366 |
| comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection. | this study aims to evaluate and compare the antimicrobial efficacy of three selected root canal irrigants (biopure mtad, metronidazole, aztreonam) against microbes commonly isolated from polymicrobial microbiota of root canal infection. | 2017 | 28435359 |
| dual-targeting small-molecule inhibitors of the staphylococcus aureus fmn riboswitch disrupt riboflavin homeostasis in an infectious setting. | riboswitches are bacterial-specific, broadly conserved, non-coding rna structural elements that control gene expression of numerous metabolic pathways and transport functions essential for cell growth. as such, riboswitch inhibitors represent a new class of potential antibacterial agents. recently, we identified ribocil-c, a highly selective inhibitor of the flavin mononucleotide (fmn) riboswitch that controls expression of de novo riboflavin (rf, vitamin b2) biosynthesis in escherichia coli. he ... | 2017 | 28434876 |
| disinfection of water inoculated with enterococcus faecalis using solar/fe(iii)edds-h2o2 or s2o8(2-) process. | in this study, the activation of h2o2 and persulfate ions induced by solar photolysis of fe(iii)edds complex were investigated in water disinfection, applying solar aops processes. the use of fe(iii)edds complex maintains iron in soluble form until slightly basic ph and so the photolysis is efficient in a large range of ph compatible with natural waters. moreover, for the first time, the impact of photogenerated hydroxyl and sulfate radicals on the inactivation of enterococcus faecalis in water ... | 2017 | 28433695 |
| diversity of integrative and conjugative elements of streptococcus salivarius and their intra- and interspecies transfer. | integrative and conjugative elements (ices) are widespread chromosomal mobile genetic elements which can transfer autonomously by conjugation in bacteria. thirteen ices with a conjugation module closely related to that of icest3 of streptococcus thermophilus were characterized in streptococcus salivarius by whole-genome sequencing. sequence comparison highlighted ice evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of the fda, rpsi, or rpmg gene) ... | 2017 | 28432093 |
| ultraviolet-c light as a means of disinfecting anesthesia workstations. | anesthesia workstations (aws) are a reservoir for pathogenic organisms potentially associated with surgical site infections. this study examined the effectiveness of the tru-d smartuvc device (tru-d llc, nashville, tn) on bioburden reduction (br) on aws. | 2017 | 28431851 |
| [effects of application of vancomycin in the early stage of patients with extremely severe burn]. | objective: to evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. methods: data of 15 patients of kunshan explosion on august 2nd, 2014, admitted to the department of intensive care in our hospital were retrospectively analyzed. the clinical efficacy of continuously intravenous dripping of vancomycin (combined with ... | 2017 | 28427133 |
| correction: chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms. | [this corrects the article doi: 10.1371/journal.pone.0174888.]. | 2017 | 28426772 |
| efficacy of three different lasers on eradication of enterococcus faecalis and candida albicans biofilms in root canal system. | the objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with enterococcus faecalis and candida albicans biofilms. | 2017 | 28426292 |
| azathioprine, mercaptopurine, and 5-aminosalicylic acid affect the growth of ibd-associated campylobacter species and other enteric microbes. | campylobacter concisus is a bacterium that is associated with inflammatory bowel disease (ibd). immunosuppressive drugs including azathioprine (aza) and mercaptopurine (mp), and anti-inflammatory drug such as 5-aminosalicylic acid (5-asa) are commonly used to treat patients with ibd. this study aimed to examine the effects of aza, mp, and 5-asa on the growth of ibd-associated bacterial species and to identify bacterial enzymes involved in immunosuppressive drug metabolism. a total of 15 bacteria ... | 2017 | 28424670 |
| antimicrobial photodynamic therapy against endodontic enterococcus faecalis and candida albicans mono and mixed biofilms in the presence of photosensitizers: a comparative study with classical endodontic irrigants. | endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. in this study, it was assessed the efficacy of antimicrobial photodynamic therapy (apdt) with the zn(ii)chlorin e6 methyl ester (zn(ii)e6me) activated by red light against monospecies and mixed biofilms of enterococcus faecalis and candida albicans. the results were compared with the ones obtained with rose bengal (rb), toluidine blue-o (tbo), the synthetic tetracationic porphyri ... | 2017 | 28424663 |
| comprehensive analysis of phospholipids and glycolipids in the opportunistic pathogen enterococcus faecalis. | enterococcus faecalis is a gram-positive, opportunistic, pathogenic bacterium that causes a significant number of antibiotic-resistant infections in hospitalized patients. the development of antibiotic resistance in hospital-associated pathogens is a formidable public health threat. in e. faecalis and other gram-positive pathogens, correlations exist between lipid composition and antibiotic resistance. resistance to the last-resort antibiotic daptomycin is accompanied by a decrease in phosphatid ... | 2017 | 28423018 |
| antimicrobial and antibiofilm activities of mta supplemented with bismuth lipophilic nanoparticles. | the objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (mta) supplemented with bismuth lipophilic nanoparticles (bisbal nps). the antimicrobial activity of the composite mta-bisbal nps was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. the cytotoxicity of mta-bisbal nps was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. mta-bisb ... | 2017 | 28420830 |
| split tasks of asymmetric nucleotide-binding sites in the heterodimeric abc exporter efrcd. | many heterodimeric atp-binding cassette (abc) exporters evolved asymmetric atp-binding sites containing a degenerate site incapable of atp hydrolysis due to noncanonical substitutions in conserved sequence motifs. recent studies revealed that nucleotide binding to the degenerate site stabilizes contacts between the nucleotide-binding domains (nbds) of the inward-facing transporter and regulates atp hydrolysis at the consensus site via allosteric coupling mediated by the d-loops. however, it is u ... | 2017 | 28417533 |
| effect of instrumentation techniques and preparation taper on apical extrusion of bacteria. | the aim of this in vitro study was to evaluate the effects of different root canal instrumentation techniques and preparation tapers on the amount of apically extruded bacteria. | 2017 | 28416303 |
| effect of bacterial biofilm on the osteogenic differentiation of stem cells of apical papilla. | although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. the aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (scap) and (2) the effect of bacterial antigens on the functional differentiation of scap into a mineralizing phenotype. | 2017 | 28416302 |
| nitric oxide charged catheters as a potential strategy for prevention of hospital acquired infections. | catheter-associated hospital-acquired infections (hai's) are caused by biofilm-forming bacteria. using a novel approach, we generated anti-infective barrier on catheters by charging them with nitric oxide (no), a naturally-produced gas molecule. no is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces. | 2017 | 28410367 |
| bacterial epidemiology and antimicrobial resistance profiles of urinary specimens of the elderly. | although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. | 2017 | 28408060 |
| preventive effects of heat-killed enterococcus faecalis strain ec-12 on mouse intestinal tumor development. | establishing effective methods for preventing colorectal cancer by so-called "functional foods" is important because the global burden of colorectal cancer is increasing. enterococcus faecalis strain ec-12 (ec-12), which belongs to the family of lactic acid bacteria, has been shown to exert pleiotropic effects, such as anti-allergy and anti-infectious effects, on mammalian cells. in the present study, we aimed to evaluate the preventive effects of heat-killed ec-12 on intestinal carcinogenesis. ... | 2017 | 28406434 |
| peptidoglycan o-acetylation increases in response to vancomycin treatment in vancomycin-resistant enterococcus faecalis. | vancomycin resistance is conferred upon vancomycin-resistant enterococci (vre) through the replacement of peptidoglycan (pg) stem terminal d-ala-d-ala with d-ala-d-lac. the d-ala-d-lac incorporation can affect both the fitness and virulence of vre. here we comprehensively investigate the changes to pg composition in vancomycin-resistant enterococcus faecalis following the growth in presence of vancomycin using liquid chromatography-mass spectrometry. using high-resolution mass spectrometry, 104 ... | 2017 | 28406232 |
| rna and a cell wall component of enterococcus faecalis ic-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line j774.1. | enterococcus faecalis is a resident lactic acid bacterium in the human intestine. its immunostimulatory action was reported to be enhanced by heat sterilization. to investigate its beneficial actions, we evaluated the ability of 10 e. faecalis strains to induce interleukin-12 (il-12) production in a mouse macrophage cell line, j774.1 and found that the strain, e. faecalis ic-1, had a potent il-12-inducing ability. furthermore, we investigated the underlying mechanism by treating ic-1 cells with ... | 2017 | 28406067 |
| antibiotic resistant airborne bacteria and their multidrug resistance pattern at university teaching referral hospital in south ethiopia. | hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. this study aims at assessing antibiotic resistant airborne bacterial isolates. | 2017 | 28403894 |
| strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic lactobacillus spp. | urinary tract infections (utis), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. probiotic bacteria, especially of the genus lactobacillus, are considered a promising preventive and/or treatment therapy against utis. in order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different lactobacillus strains (lactobacillus salivarius ucm572, l. plantarum clc17 and l. acidophilus 01) in the adheren ... | 2017 | 28402532 |
| bloodstream infections caused by enterococcus spp: a 10-year retrospective analysis at a tertiary hospital in china. | in order to discover the risk factors for 30-day mortality in bloodstream infections (bsi) caused by enterococcus spp. strains, we explored the clinical and therapeutic profile of patients with enterococcus spp. bsi and the characteristics of this condition. a total of 64 patients with bsi caused by enterococcus spp. who were treated in our hospital between 2006 and 2015 were included in the study. the clinical features of patients, microbiology, and 30-day mortality were collected from the elec ... | 2017 | 28397040 |
| enterococcus faecalis bacteriocin entv inhibits hyphal morphogenesis, biofilm formation, and virulence of candida albicans. | enterococcus faecalis, a gram-positive bacterium, and candida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. both species also are among the most important and problematic, opportunistic nosocomial pathogens. surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. we report here the identification of the e. faecalis bacteriocin, entv, produced from the entv (ef ... | 2017 | 28396417 |
| physicochemical characterization of water-soluble chitosan derivatives with singlet oxygen quenching and antibacterial capabilities. | new water-soluble chitosan derivatives (wsch) were obtained by maillard reaction (mr) between glucosamine (ga) with both low and medium molecular weight chitosans (ch). the wsch showed larger solubility than the respective ch, while their deacetylation degree (dd) decreased by approximately 12%. infrared spectroscopy experiments of wsch confirmed the formation of imine bonds after mr with intensified pyranose structure, and sugar molecules as polymer branches. however, a 6-times reduction of the ... | 2017 | 28396271 |
| wound infection secondary to snakebite. | snakebites can produce severe local and systemic septic complications as well as being associated with significant overall morbidity and even mortality. | 2017 | 28395682 |
| stochasticity among antibiotic-resistance profiles of common burn-related pathogens over a six-year period. | one of the most significant contributors to morbidity and death in patients with burns is infection, which accounts for 30%-75% of post-burn fatalities. because of concerns for the development of antibiotic resistance in burn-related pathogens, the aims of this study were to identify antibiotic resistance trends for the four most common burn-related pathogens over a six-year period. | 2017 | 28394746 |
| study on the excision and integration mediated by class 1 integron in enterococcus faecalis. | recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. however, most of the investigations focused on gram-positive bacteria with few exceptions. in this study, enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. a total of eight plasmids were subjected to establish the transformants for excision and integration test. as results showed, positive excis ... | 2017 | 28390978 |
| influence of sucrose on growth and sensitivity of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans to photodynamic therapy. | this study has evaluated the effects of photodynamic inactivation (pdi) using erythrosine as photosensitizer and green light-emitting diode (led) on biofilms of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans. we have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to pdi. biofilms were formed in suspension of 10(6) cells/ml on plates before being grown in broth culture with and without sucrose and incub ... | 2017 | 28389898 |
| prenylated flavonoids from the stems and roots of tripterygium wilfordii. | phytochemical investigation on the stems and roots of tripterygium wilfordii led to the isolation and characterization of three new prenylated flavanones, tripteryols a-c (1-3), along with (±)-5,4'-dihydroxy-2'-methoxy-6',6″-dimethypyraro-(2″,3″:7,8)-6-methyflavanone (4), and ((2s)-5,7,4'-trihydroxy-2'-methoxy-8,5'-di(3-methyl-2-butenyl)-6-methylflavanone (5). structures of the compounds 1-5 were elucidated using spectroscopic techniques, such as uv, ir, nmr (1d and 2d), and hresi-ms. tripteryol ... | 2017 | 28389278 |
| stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish. | intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ... | 2017 | 28389237 |
| a 10-year profile of enterococcal bloodstream infections at a tertiary-care hospital in japan. | the first aim of this study is to characterize the epidemiology of enterococcal bloodstream infections (bsis) at a japanese tertiary-care hospital. the second aim is to identify predictive factors for 30-day mortality. | 2017 | 28385565 |
| functional studies of e. faecalis rnase j2 and its role in virulence and fitness. | post-transcriptional control provides bacterial pathogens a method by which they can rapidly adapt to environmental change. dual exo- and endonucleolytic activities of rnase j enzymes contribute to gram-positive rna processing and decay. first discovered in bacillus subtilis, rnase j1 plays a key role in mrna maturation and degradation, while the function of the paralogue rnase j2 is largely unknown. previously, we discovered that deletion of the enterococcus faecalis rnjb gene significantly att ... | 2017 | 28384222 |
| antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. | adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ... | 2017 | 28377765 |
| poly(methyl methacrylate) surface modification for surfactant-free real-time toxicity assay on droplet microfluidic platform. | microfluidic droplet reactors have many potential uses, from analytical to synthesis. stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. here we show that a silica nanoparticle (sinp) layer coated onto a poly(methyl methacrylate) (pmma) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chain ... | 2017 | 28375597 |
| chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition. | essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ... | 2016 | 28375110 |
| biological activities of undescribed north american lichen species. | lichens provide a large array of compounds with the potential for pharmaceutical development. in the present study, extracts from three previously undescribed north american lichen species were examined for antioxidant, antibacterial and anticancer activities. | 2017 | 28369962 |
| antimicrobial activity and chemical composition of "kpètè-kpètè": a starter of benin traditional beer tchoukoutou. | the aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." to achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. the samples were tested on 29 gram + and - strains by disk diffusion method. the minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. organic ac ... | 2017 | 28367445 |
| vitreous hemorrhage as an early sign of acute bacterial endophthalmitis following intravitreal ranibizumab injection. | to report a case of acute bacterial endophthalmitis after antivascular endothelial growth factor injection with a rare presentation of vitreous hemorrhage. | 2017 | 28365853 |
| phosphotransferase systems in enterococcus faecalis og1rf enhance anti-stress capacity in vitro and in vivo. | phosphotransferase systems are common and essential in bacteria, which are in charge of sugar transportation and phosphorylation. however, phosphotransferase systems were found in recent years to be associated with environmental stress factors. this study investigated the role of the mannose/fructose/sorbose phosphotransferase systems in enterococcus faecalis og1rf in adaption to harsh environments by construction of pts mutants. more than one mannose/fructose/sorbose phosphotransferase system w ... | 2017 | 28365379 |
| [bactericidal effects and smear layer removal of er:yag-laser radiation against enterococcus faecalis in root canals]. | to evaluate the cleaning ability of er:yag laser against enterococcus faecalis in root canals. methods: the single-rooted human teeth were sterilized and inoculated with enterococcus faecalis and were randomly assigned into 2 groups. a group of teeth was irrigated with saline during root canal preparation while another group with naclo. after mechanical preparation, the two groups were randomly divided into 3 subgroups according to the different treatments: samples, laser radiation, and calcium ... | 2017 | 28364108 |
| effects of synbiotics on immunity and disease resistance of narrow-clawed crayfish, astacus leptodactylus leptodactylus (eschscholtz, 1823). | the aim of this study was to evaluate the effects of prebiotics (mannanoligosaccharide and xylooligosaccharide), probiotics (enterococcus faecalis and pediococcus acidilactici) and synbiotics for 126 days on the immune responses, hemolymph indices, antioxidant enzymes, and biological responses after a 48-hour aeromonas hydrophila exposure of sub-adult crayfish (11.45 ± 1.87 g). most antibacterial activities were observed in the shell mucus of crayfish fed a diet containing xylooligosaccharide + ... | 2017 | 28363588 |
| chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms. | propolis obtained from bee hives is a natural substance with antimicrobial properties. it is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of malaysian propolis were prepared. both the extracts displayed antimicrobial and anti-biofilm properties against enterococcus faecalis, a common bacterium associated with hospital-acquired infections. high performance liquid chromatography (hplc) analysis of propolis revealed the presence of flavonoids like kaemp ... | 2017 | 28362873 |
| rapid molecular identification of fecal origin-colonies growing on enterococcus spp.-specific culture methods. | the mei, chromocult(®) enterococci, and m-enterococcus culture-based methods used to assess water quality by the detection of enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of enterococcus spp. and (2) environmental colonies identified by 16s rrna sequencing. then, two specific-rtpcr assays targeting enterococcus spp. and enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. the me ... | 2017 | 28362305 |
| attributable mortality of hospital-acquired bloodstream infections in ireland. | to estimate the attributable mortality of hospital-acquired bloodstream infections (ha-bsi) in ireland. | 2017 | 28359546 |
| prevalence and bacterial isolates of mastitis in dairy farms in selected districts of eastern harrarghe zone, eastern ethiopia. | the study was conducted from november 2015 to april 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of eastern harrarghe zone, eastern ethiopia. the study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, california mastitis test (cmt), and isolation bacterial pathogens using standard techniques. ... | 2017 | 28352648 |
| synergistic effect of haloduracin and chloramphenicol against clinically important gram-positive bacteria. | the emergence of drug-resistant pathogens has triggered the search for more efficient antimicrobial agents and formulations for treatment of infections. in recent years, combination therapy has become one of the effective clinical practices in treating infections. the present study deals with the effect of haloduracin, a lantibiotic bateriocin and chloramphenicol against clinically important bacteria. the combined use of haloduracin and chloramphenicol resulted in remarkable synergy against a sp ... | 2017 | 28352561 |
| good bugs vs bad bugs: evaluation of inhibitory effect of selected probiotics against enterococcus faecalis. | the main goal of endodontics is the prevention of apical periodontitis. this is due to the presence of persistent pathogenic microorganisms, such as enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. this study explores shifting the established paradigm of endodontic treatment, which has focused on ... | 2017 | 28349910 |
| bacterial colonization in the apical part of extracted human teeth following root-end resection and filling: a confocal laser scanning microscopy study. | the purpose of this study was to evaluate enterococcus faecalis colonization at the apical part of root canals following root-end resection and filling using confocal laser scanning microscopy (clsm). | 2017 | 28349219 |
| primary pyogenic ventriculitis caused by neisseria meningitidis: case report and review of the literature. | background. pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. primary pyogenic ventriculitis is a rare entity and few cases have been described so far. we report the first case of primary pyogenic ventriculitis in an adult caused by neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the english literature. methods. a pubmed search was performed using the terms ependymitis, vent ... | 2017 | 28348798 |
| in vitro evaluation of the antibacterial activity of five sealers used in root canal therapy. | antibacterial activity is one of the important characteristics of an ideal root canal sealer. the aim of this study was to investigate the antibacterial activity of five different sealers against enterococcus faecalis using two different methods. | 2017 | 28348620 |
| examination of enterococcus faecalis toxin-antitoxin system toxin fst function utilizing a pheromone-inducible expression vector with tight repression and broad dynamic range. | tools for regulated gene expression in enterococcus faecalis are extremely limited. in this report, we describe the construction of an expression vector for e. faecalis, designated pcie, utilizing the pq pheromone-responsive promoter of plasmid pcf10. we demonstrate that this promoter is tightly repressed, responds to nanogram quantities of the peptide pheromone, and has a large dynamic range. to demonstrate its utility, the promoter was used to control expression of the toxic peptides of two pa ... | 2017 | 28348028 |
| characterization of vancomycin-resistant enterococcus faecalis and enterococcus faecium isolated from fresh produces and human fecal samples. | increased enterococcal infections in hospitals and multidrug-resistant and vancomycin-resistant enterococci (vre) isolated from humans, animals, and food sources raised public health concern on the presence of vre in multiple sources. we performed a comparative analysis of the antimicrobial resistance and genetics of vre isolates derived from fresh produce and human fecal samples. of 389 enterococcus isolates, 8 fecal and 3 produce isolates were resistant to vancomycin and teicoplanin; all harbo ... | 2017 | 28346839 |
| extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect. | the present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by fourier transform infrared (ft-ir) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid. | 2017 | 28344399 |
| distribution of antimicrobial resistance determinants, virulence-associated factors and crispr loci in isolates of enterococcus faecalis from various settings and genetic lineages. | enterococcus faecalis represents an important factor of hospital-associated infections (hais). the knowledge on its evolution from a commensal to an opportunistic pathogen is still limited, thus we performed a study to characterize distribution of factors that may contribute to this adaptation. using a collection obtained from various settings (hospitalized patients, community carriers, animals, fresh food, sewage, water) we investigated differences in antimicrobial susceptibility, distribution ... | 2017 | 28334141 |
| cutaneous burn injury modulates urinary antimicrobial peptide responses and the urinary microbiome. | characterization of urinary bacterial microbiome and antimicrobial peptides after burn injury to identify potential mechanisms leading to urinary tract infections and associated morbidities in burn patients. | 2017 | 28333758 |
| ecological impact of ciprofloxacin on commensal enterococci in healthy volunteers. | the ecological impact of ciprofloxacin on commensal enterococci is unknown. | 2017 | 28333351 |
| high incidence of virulence factors among clinical enterococcus faecalis isolates in southwestern iran. | over the past two decades, enterococci have emerged as an important agent responsible for hospital acquired infection. several virulence factors contribute to the adherence, colonization, evasion of the host immune response, and pathogenicity and severity of the infection. enterococcus faecalis is the most common and virulent species causing infections in hospitalized patients. the aim of the present study was to examine the prevalence of genes encoding virulence factors and antimicrobial resist ... | 2017 | 28332345 |
| porphyromonas gingivalis is the most abundant species detected in coronary and femoral arteries. | an association between oral bacteria and atherosclerosis has been postulated. a limited number of studies have used 16s rna gene sequencing-based metagenomics approaches to identify bacteria at the species level from atherosclerotic plaques in arterial walls. the objective of this study was to establish detailed oral microbiome profiles, at both genus and species level, of clinically healthy coronary and femoral artery tissues from patients with atherosclerosis. tissue specimens were taken from ... | 2017 | 28326156 |
| efficiency of the intestinal bacteria in the degradation of the toxic pesticide, chlorpyrifos. | chlorpyrifos (cp) is the most commonly used pesticide throughout the world. its widespread use in agriculture and its potential toxicity to humans from ingestion of cp contaminated food have raised concerns about its risk to health. human intestinal microflora has the ability to degrade pesticides, but the exact mechanisms involved and the metabolite end-products formed are not well understood. the primary objective of this work was to analyse the in vitro degradation of cp by five model intesti ... | 2013 | 28324568 |
| first canadian case report of kidney transplantation from an hiv-positive donor to an hiv-positive recipient. | kidney transplantation has become standard of care for carefully selected patients living with human immunodeficiency virus (hiv) and end-stage renal disease (esrd) in the highly active antiretroviral therapy (haart) era. american and european prospective cohort studies have reported similar patient and graft survival compared with hiv-negative kidney transplant recipients. despite an increased rate of acute rejection, partially due to drug interactions, hiv immunovirologic parameter generally r ... | 2017 | 28321326 |
| negative allosteric regulation of enterococcus faecalis small alarmone synthetase relq by single-stranded rna. | the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, pathogenicity, and antibiotic tolerance. we show that the tetrameric small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis is a sequence-specific rna-binding protein. relq's enzymatic and rna binding activities are subject to intricate allosteric regulation. (p)ppgpp synthesis i ... | 2017 | 28320944 |
| identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes. | bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ... | 2017 | 28320885 |
| efficacy of telavancin alone and in combination with ampicillin in a rat model of enterococcus faecalis endocarditis. | we first assessed telavancin (tlv) pharmacokinetics after a single subcutaneous dose of 35 mg/kg. the pharmacokinetic data were used to predict a tlv dose that simulates human exposure and the efficacy of tlv was then evaluated using tlv 21 mg/kg q 12 h against enterococcus faecalis og1rf (tlv mic of 0.06 μg/ml) in a rat endocarditis model with an indwelling catheter. therapy was given for 3 days with tlv, daptomycin (dap) or ampicillin (amp) monotherapy and with combinations of tlv plus amp, am ... | 2017 | 28320712 |
| effect of using different vehicles on the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate. | this study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (mta) mixed with aqueous and propylene glycol extracts of arctium lappa l. and casearia sylvestris sw. | 2017 | 28320540 |
| evaluation of the role of probiotics in endodontic treatment: a preliminary study. | the principal goal of endodontics is the prevention of periapical infection. acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as enterococcus faecalis and candida albicans harboring the root canal systems of the teeth. the concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. on the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to eva ... | 2017 | 28316949 |
| safety assessment of commensal enterococci from dogs. | enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ... | 2017 | 28316009 |
| biofilm formation capability of enterococcal strains causing urinary tract infection vis-a-vis colonisation and correlation with enterococcal surface protein gene. | data regarding differences in biofilm formation among urinary isolates of enterococci causing nosocomial infection versus asymptomatic colonisation is lacking. conflicting data are available for the role of enterococcal surface protein (esp) gene in the development of enterococcal biofilms. | 2017 | 28303818 |
| draft genome sequence of a multidrug-resistant strain of enterococcus faecalis, pm01, isolated from the nest of an american bushtit, psaltriparius minimus. | pathogenic microorganisms associated with avian nests may detrimentally impact parental health and nest success for the nest primary users, potentially neighboring avian or terrestrial species, including humans. here, we report the genome sequence of enterococcus faecalis strain pm01, isolated from a failed nest of american bushtits, psaltriparius minimus. | 2017 | 28302771 |
| evaluation of antimicrobial and antifungal efficacy of chitosan as endodontic irrigant against enterococcus faecalis and candida albicans biofilm formed on tooth substrate. | bacterial biofilms formed on the root canal wall are often difficult to remove. this study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against e. faecalis and candida albicans biofilm formed on tooth substrate. | 2017 | 28298975 |
| purulent pericarditis as a complication of bacteraemic enterococcus faecalis urinary tract infection. | purulent pericarditis is a rare clinical entity in the modern antibiotic era. the most common portal of entry is thought to be direct extension from a primary lung source and is usually caused by staphylococcus aureus, streptococcus pneumoniae or haemophilus influenzae we report the case of a man aged 69 years who presented with purulent pericarditis due to enterococcus faecalis likely caused by haematogenous spread from a urinary tract source. urgent pericardiocentesis was vital and restored hi ... | 2017 | 28298381 |