Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| reducing antibacterial development risk for gsk1322322 by exploring potential human dose regimens in nonclinical efficacy studies using immunocompetent rats. | directly testing proposed clinical dosing regimens in nonclinical studies can reduce risk during the development of novel antibacterial agents. optimal dosing regimens can be identified in animal models by testing recreated human pharmacokinetic profiles. an example of this approach is presented using continuous intravenous infusions of gsk1322322 in immunocompetent rats to evaluate recreated phase 1 human exposures in pneumonia models with streptococcus pneumoniae and haemophilus influenzae and ... | 2017 | 28807913 |
| "broad-spectrum bactericidal activity of a new bioactive grafting material (f18) against clinically important bacterial strains". | infection is the most relevant surgical complication in implant or grafting procedures. osteomyelitis and other chronic conditions pose a constant challenge in the current practice of medicine. in this context, a grafting biomaterial that possesses antibacterial properties combined with bioactivity could have great clinical impact. researchers at the vitreous materials laboratory (lamav-ufscar) recently developed a glass composition, f18, which presents an improved workability range combined wit ... | 2017 | 28807880 |
| influence of oxidative stress on the antibacterial activity of betulin, betulinic acid and ursolic acid. | contribution of reactive oxygen species and oxidative stress in the antibacterial activities of betulin, betulinic acid and ursolic acid against escherichia coli, pseudomonas aeruginosa and staphylococcus aureus was investigated. the minimum inhibitory concentrations of betulin, betulinic acid and ursolic acid against e. coli, p. aeruginosa and s. aureus are 1024-, 256- and 1024-μg/ml; 512-, 256- and 256 μg/ml; 256-, 256- and 64 μg/ml respectively. cell viability of betulin-, betulinic acid- and ... | 2017 | 28807773 |
| axially substituted silicon(iv) phthalocyanine and its quaternized derivative as photosensitizers towards tumor cells and bacterial pathogens. | axially di-(alpha,alpha-diphenyl-4-pyridylmethoxy) silicon(iv) phthalocyanine (3) and its quaternized derivative (3q) were synthesized and tested as photosensitizers against tumor and bacterial cells. these new phthalocyanines were characterized by elemental analysis, and different spectroscopic methods such as ft-ir, uv-vis, maldi-tof and (1)h nmr. the photophysical properties such as absorption and fluorescence, and the photochemical properties such as singlet oxygen generation of both phthalo ... | 2017 | 28807573 |
| antibacterial steroidal alkaloids from holarrhena antidysenteriaca. | two new steroidal alkaloids, isoconkuressine and n-formylconessimine, together with 6 known steroidal alkaloids including conkuressine, conessine, isoconessimine, conimine, conarrhimine, and funtudienine, were isolated from the seeds of holarrhena antidysenteriaca wall.ex a.dc. their intrinsic antibacterial activities and synergistic effects with penicillin and vancomycin were analyzed in methicillin sensitive staphylococcus aureus (mssa) and methicillin resistant staphylococcus aureus (mrsa). t ... | 2017 | 28807228 |
| genotypic characterization of methicillin-resistant staphylococcus aureus isolated from pigs and retail foods in china. | to investigate the genotypic diversity of methicillin-resistant staphylococcus aureus (mrsa) isolated from pigs and retail foods from different geographical areas in china and further to study the routes and rates of transmission of this pathogen from animals to food. | 2017 | 28807097 |
| simultaneous detection of 13 key bacterial respiratory pathogens by combination of multiplex pcr and capillary electrophoresis. | lower respiratory tract infections continue to pose a significant threat to human health. it is important to accurately and rapidly detect respiratory bacteria. to compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (pcr) and capillary electrophoresis (mpce) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including streptococcus pneumoniae, haemophilus influ ... | 2017 | 28807095 |
| mc-media pad sa (sanita-kun sa) for the enumeration of staphylococcus aureus in a variety of foods. | mc-media pad sa (formerly known as sanita-kun sa) is a dry rehydratable film medium for the enumeration of staphylococcus aureus. the performance of the method in a variety of foods was compared with that of iso 6888-1:1999, microbiology of food and animal feeding stuffs - horizontal method for the enumeration of coagulase-positive staphylococci (staphylococcus aureus and other species) - part 1: technique using baird-parker agar medium. the validated matrixes included pastrami, a sliced cooked ... | 2017 | 28807093 |
| incidence, prevalence, and management of mrsa bacteremia across patient populations-a review of recent developments in mrsa management and treatment. | methicillin-resistant staphylococcus aureus (mrsa) infection is still a major global healthcare problem. of concern is s. aureus bacteremia, which exhibits high rates of morbidity and mortality and can cause metastatic or complicated infections such as infective endocarditis or sepsis. mrsa is responsible for most global s. aureus bacteremia cases, and compared with methicillin-sensitive s. aureus, mrsa infection is associated with poorer clinical outcomes. s. aureus virulence is affected by the ... | 2017 | 28807042 |
| monitoring, documenting and reporting the quality of antibiotic use in the netherlands: a pilot study to establish a national antimicrobial stewardship registry. | the dutch working party on antibiotic policy is developing a national antimicrobial stewardship registry. this registry will report both the quality of antibiotic use in hospitals in the netherlands and the stewardship activities employed. it is currently unclear which aspects of the quality of antibiotic use are monitored by antimicrobial stewardship teams (a-teams) and can be used as indicators for the stewardship registry. in this pilot study we aimed to determine which stewardship objectives ... | 2017 | 28806902 |
| surface-adaptive gold nanoparticles with effective adherence and enhanced photothermal ablation of methicillin-resistant staphylococcus aureus biofilm. | biofilms that contribute to the persistent bacterial infections pose serious threats to global public health, mainly due to their resistance to antibiotics penetration and escaping innate immune attacks by phagocytes. here, we report a kind of surface-adaptive gold nanoparticles (aunps) exhibiting (1) a self-adaptive target to the acidic microenvironment of biofilm, (2) an enhanced photothermal ablation of methicillin-resistant staphylococcus aureus (mrsa) biofilm under near-infrared (nir) light ... | 2017 | 28806528 |
| microfluidic co-flow of newtonian and viscoelastic fluids for high-resolution separation of microparticles. | the microfluidic passive control of microparticles largely relies on the hydrodynamic effects of the carrier media such as newtonian fluids and viscoelastic fluids. yet the viscoelastic/newtonian interfacial effect has been scarcely investigated, especially for high-resolution particle separation. here we report a microfluidic co-flow of newtonian (water or pbs) and viscoelastic fluids (peo) for the size-dependent separation of microparticles. the co-flow condition generates a stable viscoelasti ... | 2017 | 28805872 |
| electroenhanced antimicrobial coating based on conjugated polymers with covalently coupled silver nanoparticles prevents staphylococcus aureus biofilm formation. | the incidence of hospital-acquired infections is to a large extent due to device-associated infections. bacterial attachment and biofilm formation on surfaces of medical devices often act as seeding points of infection. to prevent such infections, coatings based on silver nanoparticles (agnps) are often applied, however with varying clinical success. here, the traditional agnp-based antibacterial technology is reimagined, now forming the base for an electroenhanced antimicrobial coating. to inte ... | 2017 | 28805046 |
| value of the pitt bacteraemia score to predict short-term mortality in staphylococcus aureus bloodstream infection: a validation study. | the widely used pitt bacteraemia score (pbs) has repeatedly been described as a risk factor for short-term mortality in staphylococcus aureus bloodstream infection (bsi), but little is known about its overall predictive performance. we therefore aimed to externally validate the pbs in s. aureus bsis. | 2017 | 28804865 |
| policy implementation for methicillin-resistant staphylococcus aureus in seven european countries: a comparative analysis from 1999 to 2015. | background: policies to reduce methicillin-resistant staphylococcus aureus (mrsa) infections, both healthcare-acquired (ha-mrsa) and livestock-associated (la-mrsa) are implemented europe-wide, but evaluations are difficult for countries yet to implement such policies. a descriptive study was conducted, describing multinational mrsa rates and policy implementation, focusing on mrsa mandatory surveillance. we also investigated antibiotic use and mrsa rates and the use of veterinary antibiotics. me ... | 2017 | 28804601 |
| antibiotic therapy duration for prosthetic joint infections treated by debridement and implant retention (dair): similar long-term remission for 6 weeks as compared to 12 weeks. | the required duration of antibiotic treatment for prosthetic joint infections (pji) with debridement and retention of the implant (dair procedure) is unknown. | 2017 | 28804007 |
| depsidones and an α-pyrone derivative from simpilcillium sp. psu-h41, an endophytic fungus from hevea brasiliensis leaf. | nine previously undescribed depsidones (simplicildones a-i) and one previously undescribed α-pyrone (simplicilopyrone) were isolated from the endophytic fungus simplicillium sp. psu-h41 along with 11 known compounds. their structures were established by extensive spectroscopic analysis. simplicildone a and known botryorhodine c displayed weak antibacterial against staphylococcus aureus with equal mic values of 32 μg/ml. additionally, botryorhodine c was active against methicillin-resistant s. au ... | 2017 | 28803995 |
| the rise of methicillin resistant staphylococcus aureus: now the dominant cause of skin and soft tissue infection in central australia. | this study aimed to examine the epidemiology and treatment outcomes of community-onset purulent staphylococcal skin and soft tissue infections (ssti) in central australia. we performed a prospective observational study of patients hospitalised with community-onset purulent staphylococcal ssti (n = 160). indigenous patients accounted for 78% of cases. patients were predominantly young adults; however, there were high rates of co-morbid disease. community-associated methicillin-resistant staphyloc ... | 2017 | 28803587 |
| [gastrostomy tubes: indications and infectious complications in a tertiary hospital]. | gastrostomy tube is the best option for long-term enteral nutrition. among its limitations, infections represent the most frequent minor complication. our aim is the knowledge of the number and type of gastrostomy tubes and its main indication in our hospital. in addition, prevalence of infectious complications was studied paying attention to the main etiologic agents and their antibiotic susceptibility. | 2017 | 28803460 |
| self-defensive antibiotic-loaded layer-by-layer coatings: imaging of localized bacterial acidification and ph-triggering of antibiotic release. | self-defensive antibiotic-loaded coatings have shown promise in inhibiting growth of pathogenic bacteria adhering to biomaterial implants and devices, but direct proof that their antibacterial release is triggered by bacterially-induced acidification of the immediate environment under buffered conditions remained elusive. here, we demonstrate that staphylococcus aureus and escherichia coli adhering to such coatings generate highly localized acidification, even in buffered conditions, to activate ... | 2017 | 28803214 |
| efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces. | air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ... | 2017 | 28803189 |
| novel poly(l-lactide)/graphene oxide films with improved mechanical flexibility and antibacterial activity. | poly(l-lactic acid) (plla) is a biocompatible polyester derived from renewable sources. it is desirable to reduce its brittleness and introduce antibacterial activity for biomedical applications by using graphene oxide (go) as a structural and antibacterial agent. however, commonly used polymer/go composite synthesis methods, such as physical mixing and covalent functionalization, either cause phase segregation or compromise the intrinsic properties of go. here, a novel approach is demonstrated ... | 2017 | 28803028 |
| short communication: antioxidative and antibacterial activities on staphylococcus aureus and escherichia coli o157:h4 in milk with added ginseng marc extract fermented by lactobacillus plantarum kccm 11613p. | ginseng marc, a by-product of the extraction of fresh ginseng, is known to have bioactive compounds, but is frequently discarded as agriculture waste. the objectives of our study were to assess the antioxidative activity of fermented ginseng marc extract using lactobacillus plantarum kccm 11613p and to evaluate antibacterial activity of fermented milk with added ginseng marc extract during fermentation. after 24 h of fermentation of ginseng marc extract, the viable cell number was increased to 7 ... | 2017 | 28803022 |
| impact of rapid identification of staphylococcus aureus bloodstream infection without antimicrobial stewardship intervention on antibiotic optimization and clinical outcomes. | few studies have evaluated the clinical impact of polymerase chain reaction (pcr) for staphylococcus aureus bloodstream infections in resource-limited settings that lack direct antimicrobial stewardship intervention. this retrospective cohort study compared patients with standard microbiological identification (n=343) to those with additional identification by (pcr) (n=130). time to initiation of optimal therapy was similar between groups but substantially shorter in the pcr group for those infe ... | 2017 | 28802758 |
| presence of modic type 1 change increases risk of postoperative pyogenic discitis following decompression surgery for lumbar canal stenosis. | multicenter retrospective study. | 2017 | 28802716 |
| impact of cell wall peptidoglycan o-acetylation on the pathogenesis of staphylococcus aureus in septic arthritis. | staphylococcus aureus (s. aureus) is one of the most common pathogen causing septic arthritis. to colonize the joints and establish septic arthritis this bacterium needs to resist the host innate immune responses. lysozyme secreted by neutrophils and macrophages is an important defense protein present in the joint synovial fluids. s. aureus is known to be resistant to lysozyme due to its peptidoglycan modification by o-acetylation of n-acetyl muramic acid. in this study we have investigated the ... | 2017 | 28802679 |
| postantibiotic effect and postantibiotic sub-mic effect of ltx-109 and mupirocin on staphylococcus aureus blood isolates. | the development of new synthetic antimicrobial peptides like ltx-109 provides a new class of drugs for the treatment of staphylococcus aureus infections. we evaluated ltx-109 and mupirocin for pharmacodynamic parameters against 10 methicillin-resistant s. aureus isolates. the post antibiotic effect (pae) is defined as the length of time that bacterial growth is suppressed following a brief exposure to an antibiotic. we also determined the sub-mic effect (sme) which measures the direct effect of ... | 2017 | 28802058 |
| the key role of glutamate 172 in the mechanism of type ii nadh:quinone oxidoreductase of staphylococcus aureus. | type ii nadh:quinone oxidoreductases (ndh-2s) are membrane bound enzymes that deliver electrons to the respiratory chain by oxidation of nadh and reduction of quinones. in this way, these enzymes also contribute to the regeneration of nad(+), allowing several metabolic pathways to proceed. as for the other members of the two-dinucleotide binding domains flavoprotein (tdbdf) superfamily, the enzymatic mechanism of ndh-2s is still little explored and elusive. in this work we addressed the role of ... | 2017 | 28801048 |
| characterization of a novel endolysin lyssa11 and its utility as a potent biocontrol agent against staphylococcus aureus on food and utensils. | here we show that the lyssa11 endolysin, derived from the virulent staphylococcus aureus phage sa11, has lytic activity against staphylococcal strains. bioinformatics analysis revealed an enzymatically active chap (cysteine, histidine-dependent amidohydrolases/peptidases) domain at the n-terminus of lyssa11 that showed amidase activity. a novel cell wall binding domain (cbd) in the c-terminus could bind to a broad spectrum of staphylococcal cells. the bactericidal activity of lyssa11 was determi ... | 2017 | 28800818 |
| peptide nucleic acid-fluorescence in situ hybridization for detection of staphylococci from endophthalmitis isolates: a proof-of-concept study. | rapid identification of pathogens causing endophthalmitis may improve treatment outcomes through early administration of species-specific medication. the current study reports a new molecular application of peptide nucleic acid-fluorescence in situ hybridization (pna-fish) with staphylococcus-specific molecular pna probes for the potential rapid detection of common pathogens causing endophthalmitis. | 2017 | 28800650 |
| upregulation and identification of antibiotic activity of a marine-derived streptomyces sp. via co-cultures with human pathogens. | marine natural product drug discovery has begun to play an important role in the treatment of disease, with several recently approved drugs. in addition, numerous microbial natural products have been discovered from members of the order actinomycetales, particularly in the genus streptomyces, due to their metabolic diversity for production of biologically active secondary metabolites. however, many secondary metabolites cannot be produced under laboratory conditions because growth conditions in ... | 2017 | 28800088 |
| prevalence and microbiological characteristics of qaca/b-positive methicillin-resistant staphylococcus aureus isolates in a surgical intensive care unit. | the increasing use of chlorhexidine for methicillin-resistant staphylococcus aureus (mrsa) decolonization has raised concerns about the emergence of resistance to or tolerance of this antiseptic. we examined the frequency and characteristics of qaca/b chlorhexidine tolerance genes among mrsa isolates in a surgical intensive care unit (icu) where mrsa-colonized patients are decolonized by chlorhexidine bathing. the mrsa isolates were evaluated for chlorhexidine susceptibility, mupirocin resistanc ... | 2017 | 28799881 |
| naphtho[1,2-b]furan-4,5-dione is a potent anti-mrsa agent against planktonic, biofilm and intracellular bacteria. | naphtho[1,2-b]furan-4,5-dione (n12d) and naphtho[2,3-b]furan-4,9-dione (n23d) are furanonaphthoquinone derivatives from natural resources. we examined the antimicrobial activity of n12d and n23d against drug-resistant staphylococcus aureus. | 2017 | 28799789 |
| morphology visualization of irregular shape bacteria by electron holography and tomography. | in the current work, irregular morphology of staphylococcus aureus bacteria has been visualized by phase retrieval employing off-axis electron holography (eh) and 3d reconstruction electron tomography using high-angle annular dark field scanning transmission electron microscopy (haadf-stem). bacteria interacting with gold nanoparticles (aunp) acquired a shrunken or irregular shape due to air dehydration processing. stem imaging shows the attachment of aunp on the surface of cells and suggests an ... | 2017 | 28799676 |
| bactericidal activity of the ti-13nb-13zr alloy against different species of bacteria related with implant infection. | the ti-6al-4v alloy is one of the most commonly used in orthopedic surgery. despite its advantages, there is an increasing need to use new titanium alloys with no toxic elements and improved biomechanical properties, such as ti-13nb-13zr. prosthetic joint infections (pji) are mainly caused by gram-positive bacteria; however, gram-negative bacteria are a growing problem due to associated multidrug resistance. in this study, the bacterial adherence and viability on the ti-13nb-13zr alloy have been ... | 2017 | 28799523 |
| attaching the nora efflux pump inhibitor inf55 to methylene blue enhances antimicrobial photodynamic inactivation of methicillin-resistant staphylococcus aureus in vitro and in vivo. | antimicrobial photodynamic inactivation (apdi) uses photosensitizers (pss) and harmless visible light to generate reactive oxygen species (ros) and kill microbes. multidrug efflux systems can moderate the phototoxic effects of pss by expelling the compounds from cells. we hypothesized that increasing intracellular concentrations of pss by inhibiting efflux with a covalently attached efflux pump inhibitor (epi) would enhance bacterial cell phototoxicity and reduce exposure of neighboring host cel ... | 2017 | 28799332 |
| chemical sterilization of allograft dermal tissues. | common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. one approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. this combination of processes is an accepted method for production of sterile healthcare products as described in ansi/aami st67:2011. this article describes the validation of the peracetic acid and ethanol- ... | 2017 | 28799106 |
| mathematical model of interaction between bacteriocin-producing lactic acid bacteria and listeria. part 2: bifurcations and applications. | the big challenge for the food industry is the attending to demands for minimally processed foods, avoiding intense heat treatments and reducing the addition of chemical preservatives, but at the same time ensuring microbiological safety of these products. lactic acid bacteria are traditionally used in the production of fermented foods. they are responsible for the production of antimicrobial compounds, such as organic acids and bacteriocins, which are protein compounds with bactericidal effect ... | 2017 | 28799082 |
| prevalence of subclinical mastitis and distribution of pathogens in dairy farms of rubavu and nyabihu districts, rwanda. | a cross-sectional study was conducted from may 2016 to january 2017 in rubavu and nyabihu districts, western rwanda, aiming at estimating the prevalence of subclinical mastitis (scm) and identifying its causative bacteria. management practices and milking procedures were recorded through a questionnaire. 123 crossbreed milking cows from 13 dairy farms were randomly selected and screened for scm using california mastitis test (cmt). composite cmt positive milk samples were processed for bacterial ... | 2017 | 28798952 |
| complete genome sequence of a livestock-associated methicillin-resistant staphylococcus aureus sequence type 5 isolate from the united states. | livestock-associated methicillin-resistant staphylococcus aureus (la-mrsa) may be the largest mrsa reservoir outside the hospital setting. one concern with la-mrsa is the acquisition of novel mobile genetic elements by these isolates. here, we report the complete genome sequence of a swine la-mrsa sequence type 5 isolate from the united states. | 2017 | 28798188 |
| complete genome sequence of livestock-associated methicillin-resistant staphylococcus aureus sequence type 398 isolated from swine in the united states. | methicillin-resistant staphylococcus aureus (mrsa) colonizes and causes disease in many animal species. livestock-associated mrsa (la-mrsa) isolates are represented by isolates of the sequence type 398 (st398). these isolates are considered to be livestock adapted. this report provides the complete genome sequence of one swine-associated la-mrsa st398 isolate from the united states. | 2017 | 28798187 |
| complete genome sequence and annotation of the staphylococcus aureus strain hg001. | staphylococcus aureus is an opportunistic gram-positive pathogen responsible for a wide range of infections from minor skin abscesses to life-threatening diseases. here, we report the draft genome assembly and current annotation of the hg001 strain, a derivative of the rn1 (nct8325) strain with restored rbsu (a positive activator of sigb). | 2017 | 28798184 |
| bioactive mesoporous silica nanostructures with anti-microbial and anti-biofilm properties. | the increasing rate of antibiotic resistant bacteria associated with nosocomial infections in severely ill patients has urged the need for new antibacterial therapies. nanostructured materials represent emerging innovative approaches to controlled delivery of different antimicrobial drugs. delivery systems encapsulating natural compounds with antibacterial effects, such as essential oils have shown a great potential. herein we report the development of sio2 mesoporous nanosystems loaded with euc ... | 2017 | 28797969 |
| impact of oral gut decontamination on staphylococcus aureus colonization in patients undergoing allogeneic hematopoietic stem cell transplantation. | recipients of allogeneic hematopoietic stem cell transplantation (allo-hsct) are severely immunocompromised and at increased risk for infections. in this prospective, observational, single-center study including 110 hsct-recipients, the s. aureus colonization rate was reduced from 12% to 0% (p<0.001) following peri-transplant oral gut decontamination. no invasive s. aureus infections were observed. | 2017 | 28797807 |
| antibiotic susceptibilities and prevalence of methicillin resistant staphylococcus aureus (mrsa) isolated from bovine milk in pakistan. | the study was designed to investigate bovine milk for prevalence of an emerging zoonotic pathogen methicillin resistant staphylococcus aureus (mrsa), and in-vitro therapeutic response of various antibiotics against mrsa. nine hundred (900) milk samples were collected (half from cattle and half from buffalo) from private and public farms located in various tehsils of district faisalabad, using the convenient sampling method. milk samples were put to biochemical identification of staphylococcus au ... | 2017 | 28797802 |
| methyl propiolate and 3-butynone: starting points for synthesis of amphiphilic 1,2,3-triazole peptidomimetics for antimicrobial evaluation. | a library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less ... | 2017 | 28797773 |
| infection control measures and prevalence of multidrug-resistant organisms in non-hospital care settings in northeastern germany: results from a one-day point prevalence study. | much of the existing literature on the epidemiology of multidrug resistant bacterial organisms (mdros) and infection control measures still concentrates on hospital care settings. | 2017 | 28797758 |
| micro-textured films for reducing microbial colonization in a clinical setting. | transmission of microbes in the hospital environment occurs frequently through human interactions with high touch surfaces such as patient beds and over-bed tables. although stringent cleaning routines are implemented as a preventive measure to minimize transmission of microbes, it is desirable to have high touch surfaces made of anti-microbial materials. physical texturing of solid surfaces offers a non-bactericidal approach to control the colonization of such surfaces by microbes. this study i ... | 2017 | 28797757 |
| spinal epidural abscess: a series of 101 cases. | spinal epidural abscesses are uncommon but potentially devastating infections that often elude early diagnosis. an increasing incidence has been suggested; however, few contemporary data are available regarding risk factors and epidemiologic trends over time. | 2017 | 28797646 |
| particle engineering for intracellular delivery of vancomycin to methicillin-resistant staphylococcus aureus (mrsa)-infected macrophages. | methicillin-resistant staphylococcus aureus (mrsa) infection is a serious threat to the public health. mrsa is particularly difficult to treat when it invades host cells and survive inside the cells. although vancomycin is active against mrsa, it does not effectively kill intracellular mrsa due to the molecular size and polarity that limit its cellular uptake. to overcome poor intracellular delivery of vancomycin, we developed a particle formulation (ppzev) based on a blend of polymers with dist ... | 2017 | 28797580 |
| digestive decontamination in burn patients: a systematic review of randomized clinical trials and observational studies. | the objective of this systematic review is to assess the effect of selective digestive decontamination (sdd) or non-absorbable enteral antibiotics (ea) on mortality, the incidence of infection and its adverse effects in burn patients. | 2017 | 28797573 |
| prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients attending a secondary care hospital in zambia. | antimicrobial resistance is an increasing global health problem. very little data on resistance patterns of pathogenic bacteria in low-income countries exist. the aim of this study was to measure the prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients in the resource constraint setting of a secondary care hospital in zambia. nasal and rectal samples from 50 in- and 50 outpatients were collected. patients were randomly selected and informed consent was obtained. nas ... | 2017 | 28797299 |
| bacteriological profiling of diphenylureas as a novel class of antibiotics against methicillin-resistant staphylococcus aureus. | bacterial resistance to antibiotics remains an imposing global public health challenge. of the most serious pathogens, methicillin-resistant staphylococcus aureus (mrsa) is problematic given strains have emerged that exhibit resistance to several antibiotic classes including β-lactams and agents of last resort such as vancomycin. new antibacterial agents composed of unique chemical scaffolds are needed to counter this public health challenge. the present study examines two synthetic diphenylurea ... | 2017 | 28797064 |
| scintigraphic patterns of indium-111 oxine-white blood cell imaging of gram-negative versus gram-positive vertebral osteomyelitis. | the goal of the study was to investigate whether or not gram-negative organisms that secret anti-chemotactic factors caused the non-accumulation pattern of 111in-oxine-labeled white blood cell (111in wbc) scan. | 2017 | 28797001 |
| conjunctival bacteria flora of glaucoma patients during long-term administration of prostaglandin analog drops. | to investigate the effects of the long-term use of prostaglandin analogs for glaucoma treatment on the indigenous flora of the conjunctiva. | 2017 | 28796877 |
| methicillin-resistant staphylococcus aureus in cystic fibrosis: how should it be managed? | methicillin-resistant staphylococcus aureus (mrsa) remains prevalent in people with cystic fibrosis (cf). as chronic infection with worse pulmonary outcomes develops frequently, the organism is concerning to cf providers and patients. this review describes current epidemiology, our understanding of risk factors for mrsa infection, and relevant aspects of treatment with review of new and ongoing trials. | 2017 | 28796008 |
| synergy of a combination of nisin and citric acid against staphylococcus aureus and listeria monocytogenes. | food-borne diseases caused by pathogens, such as staphylococcus aureus and listeria monocytogenes, have long attracted attention globally from researchers, food industries, and food safety authorities. nisin (ns) is the only bacteriocin used worldwide as a generally recognised as safe (gras) food preservative, while citric acid (ca) has an unrestricted use in foods since it has gras status. in this study, synergistic interactions of ns combined with ca against s. aureus and l. monocytogenes were ... | 2017 | 28795907 |
| point-of-care fluorescence imaging predicts the presence of pathogenic bacteria in wounds: a clinical study. | bacteria in chronic wounds are invisible to the naked eye and can lead to delayed wound healing. point-of-care bacterial fluorescence imaging illuminates a wound with 405nm light, triggering bacteria to produce red fluorescence and enabling real-time bacterial localisation. prospective, single-blind clinical trials (clinicaltrials.gov #nct02682069, #nct03091361) were conducted to determine the positive predictive value (ppv) of this red fluorescence for detecting bacteria in chronic wounds. | 2017 | 28795890 |
| cold atmospheric pressure plasmas exhibit antimicrobial properties against critical bacteria and yeast species. | cold atmospheric pressure plasmas (capps) have been used to sterilise implant materials and other thermally unstable medical products and to modify chemical surfaces. this study investigates the antimicrobial effect of the gas and input power used to generate capps on microorganisms causing skin infections, such as staphylococcus aureus, pseudomonas aeruginosa, candida albicans and malassezia pachydermatis. | 2017 | 28795887 |
| development of the first gene expression system for salinicoccus strains with potential application in bioremediation of hypersaline wastewaters. | salinicoccus salsiraiae im408 (=cgmcc13032) is a novel halophilic bacterium that we isolated from the saline soil of da gang oilfield. it tolerates 60 g/l sodium chloride and up to 123 g/l (1.5 m) sodium acetate and has shown a potential application in bioremediation of wastewater with high salt and high chemical oxygen demand (cod). two plasmids, ps408-1 and ps408-2, were identified in s. salsiraiae im408, and the sequences and copy numbers of the plasmids were determined. based on these plasmi ... | 2017 | 28795221 |
| cefazolin potency against methicillin-resistant staphylococcus aureus: a microbiologic assessment in support of a novel drug delivery system for skin and skin structure infections. | despite aggressive medical and surgical management, the resolution of skin and skin structure infections is often difficult due to insufficient host response, reduced drug penetration, and a high prevalence of resistance organisms such as methicillin-resistant staphylococcus aureus (mrsa). as a result of these factors, conventional management often consists of prolonged broad-spectrum systemic antimicrobials. an alternative therapy in development, ultrasonic drug dispersion (udd), uses a subcuta ... | 2017 | 28794647 |
| in vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi. | 1-octen-3-ol, known as mushroom alcohol, is a natural product extracted from fungi and plants. its antimicrobial activities against five common food-related bacteria and two pathogenic fungi were evaluated in this paper, including staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa, fusarium tricinctum and fusarium oxysporum. the results showed that 1-octen-3-ol had a strong antibacterial activity against the tested bacteria, especially ... | 2017 | 28794307 |
| in vitro activities of tedizolid and comparator antimicrobial agents against clinical isolates of staphylococcus aureus collected in 12 countries from 2014 to 2016. | clinical isolates of staphylococcus aureus (n=3929) collected by 54 medical center laboratories in 12 countries in 2014-2016 were tested for in vitro susceptibility to tedizolid, linezolid, and 11 comparators using clinical and laboratory standards institute (clsi) broth microdilution methodology with minimum inhibitory concentrations (mics) interpreted by clsi m100-s26 (2016) criteria. all isolates of s. aureus tested were susceptible to both tedizolid (mic, ≤0.5μg/ml) and linezolid (mic, ≤4μg/ ... | 2017 | 28793964 |
| a comparison of telavancin and vancomycin for treatment of methicillin-resistant staphylococcus aureus infections: a meta-analysis. | the objective of this study was to systematically review and evaluate the efficacy and safety of telavancin and vancomycin in the treatment of infections due to methicillin-resistant staphylococcus aureus (mrsa). | 2017 | 28793957 |
| optimization of therapeutic drug monitoring of vancomycin in patients with chronic hemodialysis . | to validate a simplified vancomycin monitoring algorithm in patients on chronic hemodialysis who required intravenous vancomycin for at least 3 weeks. | 2017 | 28793955 |
| decontamination of hospital surfaces with multijet cold plasma: a method to enhance infection prevention and control? | objective to evaluate the efficacy of a multijet cold-plasma system and its efficacy in decontaminating 2 surfaces commonly found in hospitals design an in vitro study of common causes of healthcare-acquired infection methods log10 9 cultures of methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, extended spectrum β-lactamase-producing escherichia coli, and acinetobacter baumannii were applied to 5-cm2 sections of stainless steel and mattress. human serum albumin (hsa) ... | 2017 | 28793943 |
| antibacterial modification of kirschner wires with polyluteolin toward methicillin-resistant staphylococcus aureus (mrsa). | in this study we report antibacterial modification of kirschner wires (k-wires) with polyluteolin (pl) toward methicillin-resistant staphylococcus aureus (mrsa). k-wires were modified by immersing them in the luteolin-containing aqueous solution for 24 h. characterizations using scanning electron microscopy and electrochemical methods confirmed the presence of the pl coatings on the k-wires. the pl-coated k-wires were further found to show antibacterial activity toward mrsa and remained unimpair ... | 2015 | 28793478 |
| non-classical protein excretion is boosted by psmα-induced cell leakage. | release of cytoplasmic proteins into the supernatant occurs both in bacteria and eukaryotes. because the underlying mechanism remains unclear, the excretion of cytoplasmic proteins (ecp) has been referred to as "non-classical protein secretion." we show that none of the known specific protein transport systems of gram-positive bacteria are involved in ecp. however, the expression of the cationic and amphipathic α-type phenol-soluble modulins (psms), particularly of psmα2, significantly increase ... | 2017 | 28793253 |
| staphylococcal enterotoxin type r pseudogene presence in staphylococcus aureus reference and outbreak strains. | novel staphylococcal enterotoxins (ses) expressed by staphylococcus aureus strains have been described throughout the years, among these being the se protein ser. to further characterize this toxin, this research used 13 s. aureus strains previously determined to contain the se type r (ser) gene. these s. aureus isolates were evaluated using serological assays for identification of sea-see and pcr for the detection of newly described se and se-like enterotoxin genes seg-seu. pcr-based cloning wa ... | 2017 | 28792378 |
| functional redundancy of division specific penicillin-binding proteins in bacillus subtilis. | bacterial cell division involves the dynamic assembly of a diverse set of proteins that coordinate the invagination of the cell membrane and synthesis of cell wall material to create the new cell poles of the separated daughter cells. penicillin-binding protein pbp 2b is a key cell division protein in bacillus subtilis proposed to have a specific catalytic role in septal wall synthesis. unexpectedly, we find that a catalytically inactive mutant of pbp 2b supports cell division, but in this backg ... | 2017 | 28792086 |
| oxidative damage to pseudomonas aeruginosa atcc 27833 and staphylococcus aureus atcc 24213 induced by cuo-nps. | the cytotoxicity of nanoparticles (nps) and their properties are important issues in nanotechnology research. particularly, nps affect the metabolism of microorganisms due to np interactions with some biomolecules. in order to assess the mechanisms underlying nps toxicity, we studied the damage caused by copper oxide nanoparticles (cuo-nps) on staphylococcus aureus atcc 24213 and pseudomonas aeruginosa atcc 27833. spherical cuo-nps characterized by their diameter (13 ± 3 nm) were synthesized wit ... | 2017 | 28791555 |
| 59 eyes with endogenous endophthalmitis- causes, outcomes and mortality in a danish population between 2000 and 2016. | to study the epidemiology of patients with endogenous endophthalmitis in denmark. | 2017 | 28791473 |
| toxic shock syndrome caused by suture abscess with methicillin-resistant staphylococcus aureus (mrsa) with late onset after caesarean section. | toxic shock syndrome (tss) is a rare but life-threatening multisystem disease known to develop in the early postoperative period after various surgery. we report a rare case in which a patient who underwent caesarean section developed tss caused by methicillin-resistant staphylococcus aureus (mrsa) on the 39th postoperative day. she was treated with debridement because of the possible diagnosis of necrotizing soft tissue infections. culture test from the resected specimen was positive for mrsa. ... | 2017 | 28791216 |
| preconditioning with lipopolysaccharide or lipoteichoic acid protects against staphylococcus aureus mammary infection in mice. | staphylococcus aureus is one of the most causative agents of mastitis and is associated with chronic udder infections. the persistency of the pathogen is believed to be the result of an insufficient triggering of local inflammatory signaling. in this study, the preclinical mastitis model was used, aiming to evaluate if lipopolysaccharide (lps) or lipoteichoic acid (lta) preconditioning could aid the host in more effectively clearing or at least limiting a subsequent s. aureus infection. a protot ... | 2017 | 28791009 |
| modeling the combined effect of pressure and mild heat on the inactivation kinetics of escherichia coli, listeria innocua, and staphylococcus aureus in black tiger shrimp (penaeus monodon). | the high-pressure inactivation of escherichia coli, listeria innocua, and staphylococcus aureus was studied in black tiger shrimp (penaeus monodon). the processing parameters examined included pressure (300 to 600 mpa) and temperature (30 to 50°c). in addition, the pressure-hold period (0 to 15 min) was investigated, thus allowing both single-pulse pressure effects (i.e., zero holding time) and pressure-hold effects to be explored. e. coli was found to be the most sensitive strain to single-puls ... | 2017 | 28790979 |
| antibiotic resistance pattern of isolates from wound and soft tissue infections. | two-hundred and eighty bacterial isolates from wound and soft tissue infections were studied for species identification and antibiotic resistance pattern. amongst them 122 isolates were from community acquired infection and 158 were from nosocomial infections. the common community acquired pathogens were staphylococcus aureus (67.8%) and streptococcus pyogenes (10.7%), whereas staphylococcus aureus (60.1%) and e. coli (8.9%) were common in nosocomial infection. only two anaerobes (cl perfringens ... | 2000 | 28790708 |
| beta-lactam combination therapy for the treatment of staphylococcus aureus and enterococcus species bacteremia: a summary and appraisal of the evidence. | staphylococcal bacteremia and enterococcal bacteremia are prevalent in hospitalized or recently instrumented patients, and are associated with significant morbidity and mortality. they are often difficult to treat due to the pathogenicity of the organisms, poor response to antibiotics, and increasing development of multidrug resistance. therefore, there has been increasing interest in combination therapy for the treatment of these infections. the aim of this review was to summarize and assess th ... | 2017 | 28789974 |
| antimicrobial activity of chitosan-carbon nanotube hydrogels. | in the present study, we have prepared chitosan-carbon nanotube (chitosan-cnt) hydrogels by the freeze-lyophilization method and examined their antimicrobial activity. different concentrations of cnt were used in the preparation of chitosan-cnt hydrogels. these differently concentrated cnt hydrogels were chemically characterized using fourier transform-infrared spectroscopy, scanning electron microscopy and optical microscopy. the porosity of the hydrogels were found to be >94%. dispersion of ch ... | 2014 | 28788658 |
| initial bacterial adhesion on different yttria-stabilized tetragonal zirconia implant surfaces in vitro. | bacterial adhesion to implant biomaterials constitutes a virulence factor leading to biofilm formation, infection and treatment failure. the aim of this study was to examine the initial bacterial adhesion on different implant materials in vitro. four implant biomaterials were incubated with enterococcus faecalis, staphylococcus aureus and candida albicans for 2 h: 3 mol % yttria-stabilized tetragonal zirconia polycrystal surface (b1a), b1a with zirconium oxide (zro₂) coating (b2a), b1a with zirc ... | 2013 | 28788415 |
| nanostructured titanium-10 wt% 45s5 bioglass-ag composite foams for medical applications. | the article presents an investigation on the effectiveness of nanostructured titanium-10 wt% 45s5 bioglass-1 wt% ag composite foams as a novel class of antibacterial materials for medical applications. the ti-based composite foams were prepared by the combination of mechanical alloying and a "space-holder" sintering process. in the first step, the ti-10 wt% 45s5 bioglass-1 wt% ag powder synthesized by mechanical alloying and annealing mixed with 1.0 mm diameter of saccharose crystals was finally ... | 2015 | 28788008 |
| synthesis, characterization, antimicrobial studies and corrosion inhibition potential of 1,8-dimethyl-1,3,6,8,10,13-hexaazacyclotetradecane: experimental and quantum chemical studies. | the macrocylic ligand, 1,8-dimethyl-1,3,6,8,10,13-hexaazacyclotetradecane (mhacd) was synthesized by the demetallation of its freshly synthesized ni(ii) complex (nimhacd). successful synthesis of nimhacd and the free ligand (mhacd) was confirmed by various characterization techniques, including fourier transform infra-red (ft-ir), proton nuclear magnetic resonance (¹h-nmr), carbon-13 nuclear magnetic resonance ((13)c-nmr), ultraviolet-visible (uv-vis), and energy dispersive x-ray (edx) spectrosc ... | 2016 | 28787909 |
| antimicrobial properties and cytocompatibility of plga/ag nanocomposites. | the purpose of this study was to investigate the antimicrobial properties of multifunctional nanocomposites based on poly(dl-lactide-co-glycolide) (plga) and increasing concentration of silver (ag) nanoparticles and their effects on cell viability for biomedical applications. plga nanocomposite films, produced by solvent casting with 1 wt%, 3 wt% and 7 wt% of ag nanoparticles were investigated and surface properties were characterized by atomic force microscopy and contact angle measurements. an ... | 2016 | 28787836 |
| evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and pseudomonas aeruginosa biolfilm. | healthcare acquired infections (hai) pose a great threat in hospital settings and environmental contamination can be attributed to the spread of these. de-contamination and, significantly, prevention of re-contamination of the environment could help in preventing/reducing this threat. goldshield (gs5) is a novel organosilane biocide marketed as a single application product with residual biocidal activity. we tested the hypothesis that gs5 could provide longer-term residual antimicrobial activity ... | 2017 | 28787014 |
| development of ha/ag-nps composite coating from green process for hip applications. | in the present study, biological hydroxyapatite (ha) was obtained from bovine bones through a thermal process. a total of 0% and 1% of silver nanoparticles (ag-nps) synthesized from opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (aps) on a ti6al4v substrate. following this, its antimicrobial efficiency was evaluated against the following bacterial strains: escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa. this was c ... | 2017 | 28786916 |
| high prevalence of clonally diverse spa type t026 staphylococcus aureus contaminating rural eggshells. | the presence of staphylococcus aureus in poultry and poultry products, including eggs, increases its potential to enter the food chain, resulting in foodborne diseases. in this context, eggshell colonization by staphylococci may represent a risk factor. this study aimed to investigate the contamination of rural eggshell by s. aureus and to characterize the key features of the isolated strains. | 2017 | 28786785 |
| cloxacillin induced agranulocytosis: a rare adverse event of a commonly used antibiotic. | cloxacillin, a semisynthetic penicillin is a potent inhibitor of most penicillinase-producing staphylococci. use of high doses of cloxacillin for 6 weeks is recommended for the treatment of infective endocarditis caused by methicillin-susceptible staphylococcus aureus (mssa). here, we report a case of cloxacillin-induced agranulocytosis in a patient treated for mssa native tricuspid valve endocarditis, which was resolved after discontinuation of the antibiotic. this case report highlights a rare ... | 2017 | 28786715 |
| evaluation of metabolism of azo dyes and their effects on staphylococcus aureus metabolome. | dyes containing one or more azo linkages are widely applied in cosmetics, tattooing, food and drinks, pharmaceuticals, printing inks, plastics, leather, as well as paper industries. previously we reported that bacteria living on human skin have the ability to reduce some azo dyes to aromatic amines, which raises potential safety concerns regarding human dermal exposure to azo dyes such as those in tattoo ink and cosmetic colorant formulations. to comprehensively investigate azo dye-induced toxic ... | 2017 | 28786013 |
| saccharopolyspora hattusasensis sp. nov., isolated from soil. | a saccharopolyspora strain, designated cr3506(t), isolated from a soil sample collected from sungurlu, corum, turkey, was examinated using a polyphasic approach. phylogenetic analysis based on an almost-complete 16s rrna gene sequence analysis showed that the strain is closely related to the type strains of saccharopolyspora spinosa nrrl 18395(t) (99.1%), saccharopolyspora phatthalungensis nrrl b-24798(t) (98.4%) and saccharopolyspora shandongensis 88(t) (98.1%); low levels of dna-dna relatednes ... | 2017 | 28785826 |
| bioprospecting marine actinomycetes for multidrug-resistant pathogen control from rameswaram coastal area, tamil nadu, india. | a potent streptomyces bacillaris strain ram25c4 was isolated for controlling methicillin-resistant staphylococcus aureus and multidrug-resistant bacteria such as staphylococcus aureus, acinetobacter baumannii, and pseudomonas aeruginosa. a total of 131 actinomycetes were isolated from the rameswaram coastal region, tamil nadu, india. among 131 actinomycetes, maximum number of actinomycetes (55%) isolated at the distance of 3-6 m from seashore. out of 131 actinomycetes, 85% of the actinomycetes e ... | 2017 | 28785779 |
| staphylococcus aureus esx factors control human dendritic cell functions conditioning th1/th17 response. | the opportunistic pathogen staphylococcus aureus (s. aureus) is a major cause of nosocomial- and community-acquired infections. in addition, many antibiotic-resistant strains are emerging worldwide, thus, there is an urgent unmet need to pinpoint novel therapeutic and prophylactic strategies. in the present study, we characterized the impact of infection with the pandemic methicillin-resistant usa300 s. aureus strain on human primary dendritic cells (dc), key initiators and regulators of immune ... | 2017 | 28785545 |
| a high prevalence of tylosin resistance among staphylococcus aureus strains isolated from bovine mastitis. | the macrolides appear to have considerable effects for treatment of bovine mastitis because of excellent diffusion into the mammary gland, long half-life, low protein binding, high intracellular concentration and lipid solubility. acquired resistance to macrolides in staphylococcus aureus is primarily related to target-site modification through acquisition of an erm gene. in the present study the prevalence of both phenotypic and genotypic tylosin resistance in s. aureus isolates (n = 103) from ... | 2017 | 28785387 |
| impetiginized dyshidrotic eczema. | a 16 years old female patient, affected by atopic dermatitis and rhinoconjunctivitis allergica since childhood, requested a dermatologic consultation for lesions which had appeared after 3 months of local treatment with clobethasole propionate. the histological analysis confirmed the diagnosis of dyshidrotic eczema and the microbiological smears demonstrated a significant infection with staphylococcus aureus. the risk of developing corticosteroids' side-effects depends on the potency of the prod ... | 2017 | 28785355 |
| antiproliferative and antibacterial activities of cirsium scabrum from tunisia. | several cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. in the framework of a previous study conducted on eight extremophile plants from tunisia, we highlighted that the crude methanolic extract of c. scabrum, a not investigated thistle, showed moderate but quite selective cytotoxic activity against the cancerous cell line j774 compared to the noncancerous cell line wi38 (ic50 = 11.53 μ ... | 2017 | 28785293 |
| thrombin-derived host-defense peptides modulate monocyte/macrophage inflammatory responses to gram-negative bacteria. | host-defense peptides play a fundamental role in the innate immune system by modulating inflammatory responses. previously, it was shown that the thrombin derived host-defense peptide gky25 inhibits lps-induced responses of monocytes and macrophages in vitro, ex vivo, and in vivo. in this study, the effect of gky25 on the interaction of monocytes/macrophages with gram-negative bacteria was explored. electron microscopy analysis showed that fibrin slough from non-healing wounds, colonized with st ... | 2017 | 28785265 |
| detection and characterization of staphylococcus aureus and methicillin-resistant s. aureus in foods confiscated in eu borders. | the aim of the study was to evaluate the potential role of the illegal entry of food in ue in the methicillin-resistant s. aureus (mrsa) spread. we studied the prevalence and characteristics of staphylococcus aureus and mrsa isolated from foods of animal origin confiscated from passengers on flights from 45 non-eu countries from 2012 to 2015 by the border authorities at bilbao international airport (spain) and vienna international airport (austria), as well as foods from open markets close to eu ... | 2017 | 28785245 |
| a c1qdc protein (hcc1qdc6) with three tandem c1q domains is involved in immune response of triangle-shell pearl mussel (hyriopsis cumingii). | c1q-domain-containing (c1qdc) proteins are a family of proteins with a globular c1q (gc1q) domain and participate in several immune responses. in this study, a c1qdc gene was identified from the triangle-shell pearl mussel hyriopsis cumingii (designated as hcc1qdc6). this gene has a full-length cdna of 1782 bp and an open reading frame of 1,335 bp that encodes a 444-amino acid polypeptide containing three gc1q domains. hcc1qdc6 contains at least five exons and four introns. the mrna transcripts ... | 2017 | 28785225 |
| genomic surveillance reveals low prevalence of livestock-associated methicillin-resistant staphylococcus aureus in the east of england. | livestock-associated methicillin-resistant staphylococcus aureus (la-mrsa) is an emerging problem in many parts of the world. la-mrsa has been isolated previously from animals and humans in the united kingdom (uk), but the prevalence is unknown. the aim of this study was to determine the prevalence and to describe the molecular epidemiology of la-mrsa isolated in the east of england (broadly cambridge and the surrounding area). we accessed whole genome sequence data for 2,283 mrsa isolates from ... | 2017 | 28785112 |
| clonal differences in staphylococcus aureus bacteraemia-associated mortality. | the bacterium staphylococcus aureus is a major human pathogen for which the emergence of antibiotic resistance is a global public health concern. infection severity, and in particular bacteraemia-associated mortality, has been attributed to several host-related factors, such as age and the presence of comorbidities. the role of the bacterium in infection severity is less well understood, as it is complicated by the multifaceted nature of bacterial virulence, which has so far prevented a robust m ... | 2017 | 28785103 |
| ssaavs containing cassettes encoding sacas9 and guides targeting hepatitis b virus inactivate replication of the virus in cultured cells. | management of infection with hepatitis b virus (hbv) remains a global health problem. persistence of stable covalently closed circular dna (cccdna) during hbv replication is responsible for modest curative efficacy of currently licensed drugs. novel gene editing technologies, such as those based on crispr/cas9, provide the means for permanently disabling cccdna. however, efficient delivery of antiviral sequences to infected hepatocytes is challenging. a limiting factor is the large size of seque ... | 2017 | 28785016 |
| carriage frequency, phenotypic, and genotypic characteristics of methicillin-resistant staphylococcus aureus isolated from dental health-care personnel, patients, and environment. | there is limited data on methicillin-resistant staphylococcus aureus (mrsa) carriage in dental clinics. 1300 specimens from patients, health personnel, and environmental surfaces of a dental clinic in egypt were tested for mrsa. antibiotic susceptibility, biofilm formation, staphylococcal protein a (spa) typing, sccmec typing, and pcr-based assays were used to detect meca, mecc, vana, panton-valentine leukocidin toxin (pvl), and toxic shock syndrome toxin-1 (tst) genes. among 34 meca-positive mr ... | 2017 | 28784993 |