Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| seroprevalence of q fever, brucellosis, and bluetongue in selected provinces in lao people's democratic republic. | this study has determined the proportional seropositivity of two zoonotic diseases, q fever and brucellosis, and bluetongue virus (btv) which is nonzoonotic, in five provinces of lao people's democratic republic (pdr) (loungphabang, luangnumtha, xayaboury, xiengkhouang, and champasak, and vientiane province and vientiane capital). a total of 1,089 samples from buffalo, cattle, pigs, and goats were tested, with seropositivity of btv (96.7%), q fever (1.2%), and brucellosis (0.3%). the results of ... | 2016 | 27430548 |
| [a second branchial arch cyst superinfected by coxiella burnetii]. | 2016 | 27427281 | |
| right on q: genetics begin to unravel coxiella burnetii host cell interactions. | invasion of macrophages and replication within an acidic and degradative phagolysosome-like vacuole are essential for disease pathogenesis by coxiella burnetii, the bacterial agent of human q fever. previous experimental constraints imposed by the obligate intracellular nature of coxiella limited knowledge of pathogen strategies that promote infection. fortunately, new genetic tools facilitated by axenic culture now allow allelic exchange and transposon mutagenesis approaches for virulence gene ... | 2016 | 27418426 |
| age-related risk factors for bacterial aetiology in community-acquired pneumonia. | the objective of this study was to evaluate the effect of age and comorbidities, smoking and alcohol use on microorganisms in patients with community-acquired pneumonia (cap). | 2016 | 27417291 |
| anaplasma marginale and theileria annulata in questing ticks from portugal. | ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ... | 2016 | 27394441 |
| mediterranean spotted fever-like illness in sardinia, italy: a clinical and microbiological study. | rickettsioses represent a group of emerging infectious diseases in europe. climate changes and the anthropization of rural environment have favored vectors' biological cycle and geographic spread. in sardinia, mediterranean spotted fever (msf) is endemic and represents an important public health problem. | 2016 | 27380385 |
| chronic q fever in alberta: a case of coxiella burnetii mycotic aneurysm and concomitant vertebral osteomyelitis. | chronic q fever is a potentially life-threatening infection from the intracellular, gram-negative coxiella burnetii. it presents most commonly as endocarditis or vascular infection in people with underlying cardiac or vascular disease. we discuss a case of a 67-year-old male with coxiella burnetii vascular infection of a perirenal abdominal aortic graft. the patient had a history of an abdominal aortic aneurysm (aaa) repair 5 years earlier. he presented with a 12 × 6 × 8 cm perirenal pseudoaneur ... | 2016 | 27366178 |
| use of 18f-fdg pet/ct for diagnosis of vascular graft infection with spread to sternum caused by coxiella burnetii. | 2016 | 27356964 | |
| [unusual evolution of q fever. case report]. | 2017 | 27345581 | |
| interactions between the coxiella burnetii parasitophorous vacuole and the endoplasmic reticulum involve the host protein orp1l. | coxiella burnetii is a gram-negative intracellular bacterium that forms a large, lysosome-like parasitophorous vacuole (pv) essential for bacterial replication. host membrane lipids are critical for the formation and maintenance of this intracellular niche, yet the mechanisms by which coxiella manipulates host cell lipid metabolism, trafficking and signalling are unknown. oxysterol-binding protein-related protein 1 long (orp1l) is a mammalian lipid-binding protein that plays a dual role in chole ... | 2017 | 27345457 |
| [spleen infarction diagnosed in the health centre … and it turned out to be q fever]. | 2017 | 27344580 | |
| rapid molecular diagnosis of infective aortic valve endocarditis caused by coxiella burnetii. | we describe a case of q-fever endocarditis with severe destruction of the aortic valve with perivalvular abscess formation and cardiac failure. the patient needed urgent operative treatment and postoperative critical care. all specimens sent for microbiological examination were negative. molecular analysis, including fluorescence in situ hybridization of aortic valve tissue combined with pcr and sequencing, led to the correct diagnosis and to appropriate anti-infective treatment. the patient sub ... | 2016 | 27339147 |
| genotypes of coxiella burnetii in wildlife: disentangling the molecular epidemiology of a multi-host pathogen. | evidences point to a relevant role of wildlife in the ecology of coxiella burnetii worldwide. the lack of information on c. burnetii genotypes in wildlife prevents tracing-back clinical animal and human q fever cases with potential wildlife origin. to compare c. burnetii genotypes circulating in wildlife, livestock and humans, 107 samples from red deer, european wild rabbit, racoon, small mammals, goat and sheep were genotyped by polymerase chain reaction and reverse line blot hybridization. gen ... | 2016 | 27336914 |
| nuclear trafficking of the anti-apoptotic coxiella burnetii effector protein ankg requires binding to p32 and importin-α1. | the obligate intracellular bacterium coxiella burnetii causes the zoonotic disease q-fever. coxiella pathogenesis depends on a functional type iv secretion system (t4ss). the t4ss effector ankg inhibits pathogen-induced host cell apoptosis, which is believed to be important for the establishment of a persistent infection. however, the mode of action of ankg is not fully understood. we have previously demonstrated that binding of ankg to p32 is crucial for migration of ankg into the nucleus and t ... | 2017 | 27328359 |
| the type iv secretion system effector protein cira stimulates the gtpase activity of rhoa and is required for virulence in a mouse model of coxiella burnetii infection. | coxiella burnetii, the etiological agent of q fever in humans, is an intracellular pathogen that replicates in an acidified parasitophorous vacuole derived from host lysosomes. generation of this replicative compartment requires effectors delivered into the host cell by the dot/icm type ivb secretion system. several effectors crucial for c. burnetii intracellular replication have been identified, but the host pathways coopted by these essential effectors are poorly defined, and very little is kn ... | 2016 | 27324482 |
| prevalence of rickettsia species in dermacentor variabilis ticks from ontario, canada. | relatively little is known about the prevalence of rickettsial species in dermacentor ticks in eastern canada. in this study, dermacentor ticks from the province of ontario, canada, were tested for the presence of spotted fever group rickettsial (sfgr) species, coxiella burnetii and francisella tularensis. rickettsia rickettsii was not detected in any ticks tested, but r. montanensis was detected at a prevalence of 2.2% in d. variabilis (17/778). two other sfgr species, r. parkeri and candidatus ... | 2016 | 27318438 |
| atypical aetiology in patients hospitalised with community-acquired pneumonia is associated with age, gender and season; a data-analysis on four dutch cohorts. | microorganisms causing community-acquired pneumonia (cap) can be categorised into viral, typical and atypical (legionella species, coxiella burnetii, mycoplasma pneumoniae, and chlamydia species). extensive microbiological testing to identify the causative microorganism is not standardly recommended, and empiric treatment does not always cover atypical pathogens. in order to optimize epidemiologic knowledge of cap and to improve empiric antibiotic choice, we investigated whether atypical microor ... | 2016 | 27317257 |
| genotyping and axenic growth of coxiella burnetii isolates found in the united states environment. | coxiella burnetii is a gram-negative bacterium that is the etiologic agent of the zoonotic disease q fever. common reservoirs of c. burnetii include sheep, goats, and cattle. these animals shed c. burnetii into the environment, and humans are infected by inhalation of aerosols. a survey of 1622 environmental samples taken across the united states in 2006-2008 found that 23.8% of the samples contained c. burnetii dna. to identify the strains circulating in the u.s. environment, dna from these env ... | 2016 | 27304166 |
| murine alveolar macrophages are highly susceptible to replication of coxiella burnetii phase ii in vitro. | coxiella burnetii is a gram-negative bacterium that causes q fever in humans. q fever is an atypical pneumonia transmitted through inhalation of contaminated aerosols. in mammalian lungs, c. burnetii infects and replicates in several cell types, including alveolar macrophages (ams). the innate immunity and signaling pathways operating during infection are still poorly understood, in part because of the lack of relevant host cell models for infection in vitro in the study described here, we inves ... | 2016 | 27297388 |
| chronic recurrent multifocal q fever osteomyelitis in children: an emerging clinical challenge. | clinical disease caused by coxiella burnetii occurs infrequently in children. chronic q fever is particularly uncommon and endocarditis is rarely seen. a small number of cases of q fever osteomyelitis have been described but the pathophysiology is not well understood and optimal treatment is unknown. | 2016 | 27294309 |
| novel genotypes of coxiella burnetii identified in isolates from australian q fever patients. | coxiella burnetii, the causative agent of q fever, was first discovered in australia in 1937. however, little is known about the strains of c. burnetii present in this country. in this study, six published genotyping methods were applied to 42 isolates from australian patients with acute (n=39) and chronic (n=3) q fever. all the isolates contained the plasmid qprs and lacked the acute disease antigen a (adaa) gene. two methods of genotyping based on single nucleotide polymorphisms (snps) also fa ... | 2016 | 27283085 |
| community-acquired pneumonia related to intracellular pathogens. | community-acquired pneumonia (cap) is associated with high rates of morbidity and mortality worldwide; the annual incidence of cap among adults in europe has ranged from 1.5 to 1.7 per 1000 population. intracellular bacteria are common causes of cap. however, there is considerable variation in the reported incidence between countries and change over time. the intracellular pathogens that are well established as causes of pneumonia are legionella pneumophila, mycoplasma pneumoniae, chlamydophila ... | 2016 | 27276986 |
| febrile patients admitted to remote hospitals in northeastern kenya: seroprevalence, risk factors and a clinical prediction tool for q-fever. | q fever in kenya is poorly reported and its surveillance is highly neglected. standard empiric treatment for febrile patients admitted to hospitals is antimalarials or penicillin-based antibiotics, which have no activity against coxiella burnetii. this study aimed to assess the seroprevalence and the predisposing risk factors for q fever infection in febrile patients from a pastoralist population, and derive a model for clinical prediction of febrile patients with acute q fever. | 2016 | 27260261 |
| the first serological study of coxiella burnetii among pregnant women in iran. | q fever is a zoonotic disease caused by coxiella burnetii. there is no information about this disease in pregnant women in iran. the aim of this study was to investigate the seroprevalence of c. burnetii infection among pregnant women in southwestern (ahvaz) and northern (parsabad) iran and further to comparison its prevalence in normal and abnormal pregnancies. | 2016 | 27252922 |
| correction for sandoz et al., complementation of arginine auxotrophy for genetic transformation of coxiella burnetii by use of a defined axenic medium. | 2016 | 27247311 | |
| sternoclavicular joint infection caused by coxiella burnetii: a case report. | few cases of q fever osteoarticular infection have been reported, with chronic osteomyelitis as the most common manifestation of q fever osteoarticular infection. here we present the case of a sternoclavicular joint infection caused by coxiella burnetii and localized by positron emission tomography scanning. | 2016 | 27246557 |
| detection and genotyping of coxiella burnetii and coxiella-like bacteria in horses in south korea. | coxiella burnetii and coxiella-like bacteria (clb) are genetically and ecologically distinct despite some genetic similarities. furthermore, clb are exceptionally diverse and widespread in ticks, but rarely detected in domestic animals. since coxiella bacteria can be transmitted from infected horses by inhalation or by coming in contact with ticks during activities such as horseback riding, it is necessary to study their prevalence. to the best of our knowledge, this is the first large-scale nat ... | 2016 | 27244230 |
| serological and molecular evidence of q fever in domestic ruminants in bangladesh. | the objective of this study was to know the herd and animal level prevalence of q fever in domestic ruminants in some selected districts in bangladesh. randomly collected 111 bulk milk and 94 sera samples of cattle, sheep, and goats were tested by indirect elisa (ielisa). dna extracted from 23 aborted fetal membranes was analyzed by real time (rt) pcr. the positive cut-off value of ielisa in bulk milk and individual animal sera was ≥30% and ≥40%, respectively. the overall herd level prevalence o ... | 2016 | 27239369 |
| [epidemiologic situation by natural-foci infections in the crimea federal district in 2014-2015]. | analysis of epidemic manifestations of natural-foci infections (nfi), clarification of spectrum of their causative agents, determination of epizootic activity of natural foci in the crimea federal district (kfd). | 2016 | 27228673 |
| coxiella burnetii effector cvpb modulates phosphoinositide metabolism for optimal vacuole development. | the q fever bacterium coxiella burnetii replicates inside host cells within a large coxiella-containing vacuole (ccv) whose biogenesis relies on the dot/icm-dependent secretion of bacterial effectors. several membrane trafficking pathways contribute membranes, proteins, and lipids for ccv biogenesis. these include the endocytic and autophagy pathways, which are characterized by phosphatidylinositol 3-phosphate [pi(3)p]-positive membranes. here we show that the c. burnetii secreted effector coxie ... | 2016 | 27226300 |
| fatigue following acute q-fever: a systematic literature review. | long-term fatigue with detrimental effects on daily functioning often occurs following acute q-fever. following the 2007-2010 q-fever outbreak in the netherlands with over 4000 notified cases, the emphasis on long-term consequences of q-fever increased. the aim of this study was to provide an overview of all relevant available literature, and to identify knowledge gaps regarding the definition, diagnosis, background, description, aetiology, prevention, therapy, and prognosis, of fatigue followin ... | 2016 | 27223465 |
| coxiella burnetii genotypes in iberian wildlife. | to investigate if coxiella burnetii, the causative agent of q fever, genotypes circulating in wildlife are associated with those infecting livestock and humans, multiple-locus variable number tandem-repeat analysis (mlva-6-marker) was carried out over c. burnetii obtained from red deer (cervus elaphus), eurasian wild boar (sus scrofa), european wild rabbit (oryctolagus cuniculus), black rat (rattus rattus), and wood mouse (apodemus sylvaticus). mlva typing was performed by using six variable loc ... | 2016 | 27216529 |
| select public health and communicable disease lessons learned during operations iraqi freedom and enduring freedom. | history has taught us that the threat of communicable diseases to operational readiness should not be underestimated. the unique operational challenges of a decade at war in southwest asia have left us with many new lessons about prevention and mitigation of disease. the successes of military immunization programs demonstrated the successful application of military science to modern combat. historic maladies such as tuberculosis and malaria continue to challenge our army health leadership while ... | 2017 | 27215886 |
| the use of guidelines for lower respiratory tract infections in tanzania: a lesson from kilimanjaro clinicians. | evaluations of the guidelines for the management of lower respiratory tract infections (lrti) sub-saharan africa, particularly in tanzania is scant. | 2017 | 27213093 |
| false detection of coxiella burnetii-what is the risk? | 2016 | 27190242 | |
| first serologic survey of q fever in free-range yaks in china. | the objective of the present investigation was to determine the seroprevalence of coxiella burnetii infection in free-range yaks in china. a total of 552 serum samples were collected from yaks in gansu province, northwest china between april 2013 and january 2014, and antibodies against c. burnetii were evaluated using enzyme-linked immunosorbent assay (elisa). overall, 13.59% (75/552, 95% ci: 10.73-16.45) of the examined animals were positive for c. burnetii antibodies. there was no significant ... | 2015 | 27175178 |
| seroprevalence of q fever (coxiellosis) in small ruminants of two districts in punjab, pakistan. | coxiellosis caused by coxiella burnetii is a cosmopolitan zoonosis, which causes significant losses through abortions and stillbirths in small ruminants. a cross-sectional seroprevalence study was conducted in two major sheep and goat farming districts of punjab (layyah and muzaffargarh), pakistan. in total, 542 small ruminants (271 sheep and goats each) of both sexes (60 males and 482 females) of different age groups from 104 flocks (52 flocks of either species) were randomly selected for the c ... | 2016 | 27172109 |
| psoas abscess and chronic q fever: a contiguous or hematogenous complication? a case report and literature review. | few cases of psoas abscesses (pa) during chronic q fever have been reported, and the route of transmission remains unknown. here, we report a new case and have performed a systematic literature review to determinate the spreading route of this complication. medline, embase and web of science were searched. local spreading was supported by endocarditis exclusion, evidence of vascular infection and absence of distantly infected sites. among 275 retrieved references, 179 were initially rejected, an ... | 2016 | 27167531 |
| the development of lyophilized loop-mediated isothermal amplification reagents for the detection of coxiella burnetii. | coxiella burnetii, the agent causing q fever, is an obligate intracellular bacterium. pcr based diagnostic assays have been developed for detecting c. burnetii dna in cell cultures and clinical samples. pcr requires specialized equipment and extensive end user training, and therefore, it is not suitable for routine work especially in a resource-constrained area. we have developed a loop-mediated isothermal amplification (lamp) assay to detect the presence of c. burnetii in patient samples. this ... | 2016 | 27167168 |
| mouse model of coxiella burnetii aerosolization. | coxiella burnetii is mainly transmitted by aerosols and is responsible for multiple-organ lesions. animal models have shown c. burnetii pathogenicity, but long-term outcomes still need to be clarified. we used a whole-body aerosol inhalation exposure system to mimic the natural route of infection in immunocompetent (balb/c) and severe combined immunodeficient (scid) mice. after an initial lung inoculum of 10(4) c. burnetii cells/lung, the outcome, serological response, hematological disorders, a ... | 2016 | 27160294 |
| q fever in french guiana: tip of the iceberg or epidemiological exception? | 2016 | 27149385 | |
| secondary aortoesophageal fistula associated with aneurysmal graft infection by coxiella burnetii. | aortoesophageal fistula is a rare and serious condition that carries a high mortality rate. we present a case of overt gastrointestinal bleeding from an aortoesophageal fistula in a patient with chronic infection of an endovascular prosthesis with coxiella burnetii. | 2016 | 27144194 |
| endemic q fever in new south wales, australia: a case series (2005-2013). | q fever is endemic in australia, and during the period 2005-2013 our laboratory diagnosed 379 cases in new south wales. to evaluate clinical symptoms, epidemiology, mode of diagnosis, antibody profiles, and treatment, a subset of 160 (42%) q fever cases were analyzed in detail following the return of a questionnaire by the patient's doctor and from their laboratory reports. overall, 82% patients were male and predominantly middle aged. the majority of patients (89%) had animal contact among whic ... | 2016 | 27139451 |
| bayesian validation of the indirect immunofluorescence assay and its superiority to the enzyme-linked immunosorbent assay and the complement fixation test for detecting antibodies against coxiella burnetii in goat serum. | although many studies have reported the indirect immunofluorescence assay (ifa) to be more sensitive in detection of antibodies to coxiella burnetii than the complement fixation test (cft), the diagnostic sensitivity (dse) and diagnostic specificity (dsp) of the assay have not been previously established for use in ruminants. this study aimed to validate the ifa by describing the optimization, selection of cutoff titers, repeatability, and reliability as well as the dse and dsp of the assay. bay ... | 2016 | 27122484 |
| serological survey of coxiella burnetii at the wildlife-livestock interface in the eastern pyrenees, spain. | coxiella burnetii is a zoonotic bacterium that infects a wide range of animal species and causes the disease q fever. both wild and domestic ruminants may be relevant in the epidemiology of c. burnetii infection. in order to investigate the significance of the ruminant host community in the alpine and subalpine ecosystems of the eastern pyrenees, northeastern spain, in the epidemiology of q fever, a serological survey was performed on samples from 599 wild and 353 sympatric domestic ruminants. | 2016 | 27121001 |
| q fever: a contemporary case series from a belgian hospital. | q fever is a global zoonosis that can cause both acute and chronic infections in humans through aerogenic transmission. although q fever was discovered already 80 years ago, this infectious disease remains largely unknown. we studied a case series in a belgian tertiary care hospital. | 2016 | 27118360 |
| co-circulation of plasmodium and bacterial dnas in blood of febrile and afebrile children from urban and rural areas in gabon. | malaria is considered to be the most common etiology of fever in sub-saharan africa while bacteremias exist but are under assessed. this study aimed to assess bacteremias and malaria in children from urban and rural areas in gabon. dna extracts from blood samples of 410 febrile and 60 afebrile children were analyzed using quantitative polymerase chain reaction. plasmodium spp. was the microorganism most frequently detected in febrile (78.8%, 323/410) and afebrile (13.3%, 8/60) children, (p < 0.0 ... | 2016 | 27114297 |
| first complete genome sequence of the dutch veterinary coxiella burnetii strain nl3262, originating from the largest global q fever outbreak, and draft genome sequence of its epidemiologically linked chronic human isolate nlhu3345937. | the largest global q fever outbreak occurred in the netherlands during 2007 to 2010. goats and sheep were identified as the major sources of disease. here, we report the first complete genome sequence of italic! coxiella burnetiigoat outbreak strain nl3262 and that of an epidemiologically linked chronic human strain, both having the outbreak-related italic! cbnl01multilocus variable-number tandem-repeat analysis (mlva) genotype. | 2016 | 27103714 |
| disease burden of 32 infectious diseases in the netherlands, 2007-2011. | infectious disease burden estimates provided by a composite health measure give a balanced view of the true impact of a disease on a population, allowing the relative impact of diseases that differ in severity and mortality to be monitored over time. this article presents the first national disease burden estimates for a comprehensive set of 32 infectious diseases in the netherlands. | 2016 | 27097024 |
| seroprevalence of infectious diseases in saiga antelope (saiga tatarica tatarica) in kazakhstan 2012-2014. | 286 serum samples were collected from three sub-populations of saiga in kazakhstan (betpakdala, ustyurt and volga-ural) between 2012 and 2014, and were tested for the presence of antibodies to brucella spp., bluetongue virus, peste des petits ruminants (ppr) virus, akabane virus, schmallenberg virus, chlamydophila, toxoplasma, mycobacterium avium subspecies paratuberculosis and coxiella burnetii (q fever). seropositives to coxiella burnetii of saiga were detected and the adjusted seroprevalence ... | 2016 | 27094147 |
| [varicelliform exanthema during a q fever]. | 2017 | 27093888 | |
| coxiella burnetii dormancy in a fatal ten-year multisystem dysfunctional illness: case report. | in a previous study of a q fever outbreak in birmingham, our group identified a non-infective complex of coxiella burnetii (c.b.) antigens able to survive in the host and provoked aberrant humoral and cell-mediated immunity responses. the study led to recognition of a possible pathogenic link between c.b. infection and subsequent long-term post q fever fatigue syndrome (qfs). this report presents an unusually severe case of c.b. antigen and dna detection in post-mortem specimens from a patient w ... | 2016 | 27091026 |
| q fever, scrub typhus, and rickettsial diseases in children, kenya, 2011-2012. | to increase knowledge of undifferentiated fevers in kenya, we tested paired serum samples from febrile children in western kenya for antibodies against pathogens increasingly recognized to cause febrile illness in africa. of patients assessed, 8.9%, 22.4%, 1.1%, and 3.6% had enhanced seroreactivity to coxiella burnetii, spotted fever group rickettsiae, typhus group rickettsiae, and scrub typhus group orientiae, respectively. | 2016 | 27088502 |
| molecular survey on zoonotic tick-borne bacteria and chlamydiae in feral pigeons (columba livia domestica). | to determine the presence of zoonotic tick-borne bacteria in feral pigeons (columba livia domestica) from urban areas. | 2016 | 27086148 |
| surgical and antimicrobial management of a thoracic aortic aneurysm due to q fever: a case report and brief review. | coxiella burnetii, the etiologic agent of q fever, has been associated with vascular infection and aneurysm formation. we report the case of a 36-year-old woman from iraq who presented with long-standing malaise as well as vague chest and shoulder discomfort and was found to have a saccular aneurysm of the descending thoracic aorta. serology assays were positive for chronic c burnetii infection. she was treated with successful aneurysm resection and aortic replacement with a rifampin-impregnated ... | 2016 | 27075992 |
| waning population immunity prior to a large q fever epidemic in the south of the netherlands. | historical survey data suggest that the seroprevalence of antibodies against coxiella burnetii in the general population of the netherlands decreased from more than 40% in 1983 to 2·4% in 2007, just before the start of the large 2007-2010 q fever epidemic. to assess whether the sharp decline in seroprevalence was real, we performed a cross-sectional study using historical samples. we tested samples using a contemporary commercial indirect immunofluorescence assay. in plasma samples from the sout ... | 2016 | 27075042 |
| type i interferon counters or promotes coxiella burnetii replication dependent on tissue. | coxiella burnetii is an intracellular pathogen and the cause of q fever. gamma interferon (ifn-γ) is critical for host protection from infection, but a role for type i ifn in c. burnetii infection has not been determined. type i ifn supports host protection from a related pathogen, legionella pneumophila, and we hypothesized that it would be similarly protective in c. burnetii infection. in contrast to our prediction, ifn-α receptor-deficient (ifnar(-/-)) mice were protected from c. burnetii-ind ... | 2016 | 27068091 |
| q fever is an old and neglected zoonotic disease in kenya: a systematic review. | q fever is a neglected zoonosis caused by the bacterium coxiella burnetii. the knowledge of the epidemiology of q fever in kenya is limited with no attention to control and prevention programs. the purpose of this review is to understand the situation of q fever in human and animal populations in kenya in the past 60 years, and help identify future research priorities for the country. | 2016 | 27048480 |
| spread of coxiella burnetii between dairy cattle herds in an enzootic region: modelling contributions of airborne transmission and trade. | q fever, a worldwide zoonotic disease caused by coxiella burnetii, is a looming concern for livestock and public health. epidemiological features of inter-herd transmission of c. burnetii in cattle herds by wind and trade of cows are poorly understood. we present a novel dynamic spatial model describing the inter-herd regional spread of c. burnetii in dairy cattle herds, quantifying the ability of airborne transmission and animal trade in c. burnetii propagation in an enzootic region. among all ... | 2016 | 27048416 |
| q fever endocarditis presenting with superior mesenteric artery embolism and renal infarction. | q fever is a zoonotic disease with a reservoir in mammals, birds, and ticks. acute cases in human beings can be asymptomatic, or they can present with a flu-like illness, pneumonia, or hepatitis. approximately 5% of cases progress to chronic q fever. endocarditis, the most typical manifestation of chronic q fever, is usually associated with small vegetations that occur in patients who have had prior valvular damage or who are immunocompromised. we present what we think is the first reported case ... | 2016 | 27047296 |
| [tick-borne diseases in poland: prevalence and difficulties in diagnostics]. | the article presents an overview of diagnostics of tick-borne diseases in poland, which form one of the most prevalent group of occupational illnesses in the polish area. this is a current issue due to a constantly growing number of tick-borne infections, i.e., lyme borreliosis, tick-borne encephalitis, tularemia, q fever, human granulocytic anaplasmosis and babesiosis. the scale of the problem is well illustrated by the latest reports of the polish national institute of public health - national ... | 2016 | 27044720 |
| modelling the risk of transfusion transmission from travelling donors. | the eufrat (european up-front risk assessment tool) was developed as an online risk assessment tool ( http://eufrattool.ecdc.europa.eu ) to help decision-makers assess the transmission risk of emerging infectious diseases (eid) through blood transfusion. the aim of this study is to extend the methodology developed in the eufrat project to quantify the transfusion transmission (tt) risk from travelling donors. | 2016 | 27038919 |
| the natural history of acute q fever: a prospective australian cohort. | a detailed description of the natural history of acute q fever, caused by infection with coxiella burnetii, aim: : to significantly increase understanding of the illness. | 2016 | 27037328 |
| [usefulness of the early molecular diagnosis of q fever and rickettsial diseases in patients with fever of intermediate duration]. | most cases of fever of intermediate duration (fdi) in spain are associated with infectious diseases (mainly q fever and rickettsia infections). in clinical practice, the causal diagnosis of these entities is based on immunodiagnostic techniques, which are of little help in the early stages. therefore, the aim of this study was to evaluate the usefulness of molecular techniques for the early diagnosis of q fever and rickettsia diseases in patients with fdi. a pcr method was used to detect the pre ... | 2016 | 27026285 |
| coxiella burnetii infects primary bovine macrophages and limits their host cell response. | although domestic ruminants have long been recognized as the main source of human q fever, little is known about the lifestyle that the obligate intracellular gram-negative bacterium coxiella burnetii adopts in its animal host. because macrophages are considered natural target cells of the pathogen, we established primary bovine monocyte-derived macrophages (mdm) as an in vitro infection model to study reservoir host-pathogen interactions at the cellular level. in addition, bovine alveolar macro ... | 2016 | 27021246 |
| disappearance of fdg uptake on pet scan after antimicrobial therapy could help for the diagnosis of coxiella burnetii spondylodiscitis. | 2016 | 27015880 | |
| treatment and prophylactic strategy for coxiella burnetii infection of aneurysms and vascular grafts: a retrospective cohort study. | coxiella burnetii vascular infections continue to be very severe diseases and no guidelines exist about their prevention. in terms of treatment, the benefit of the surgical removal of infected tissues has been suggested by 1 retrospective study.we present a case of a c burnetii abdominal aortic graft infection for which we observed a dramatic clinical and biological recovery after surgery. we thus performed a retrospective cohort study to evaluate the impact of surgery on survival and serologica ... | 2016 | 27015164 |
| molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria. | in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ... | 2016 | 27012917 |
| lyophilization to improve the sensitivity of qpcr for bacterial dna detection in serum: the q fever paradigm. | quantitative real-time pcr (qpcr) on serum provides significant added value to the diagnosis of q fever, mainly at the acute stage of the disease in seronegative patients and in patients with endocarditis. we evaluated the benefits of coxiella burnetii dna concentration in serum by lyophilization to improve qpcr sensitivity. the detection limit of qpcr was determined by comparing six 10-fold dilutions of serum (calibrated with 104 bacteria ml-1) with and without lyophilization. we also tested, a ... | 2016 | 27008653 |
| serological and molecular evidence of coxiella burnetii in samples from humans and animals in china. | coxiella burnetii is the agent of q fever, a worldwide zoonosis. to add to the available knowledge of the disease in china, c. burnetti infections were investigated in convenience samples from five animal species and humans from yangzhou, jiangsu province, eastern china. | 2016 | 27007523 |
| analysis of seroprevalence against coxiella burnetii in a sample of farm workers in western sicily. | little is known about the development of chronic q fever caused by coxiella burnetii in occupational risk groups and in the general population in italy, as well as in many countries in the world. the aim of this study was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in ... | 2016 | 27007520 |
| the ecological niche of dermacentor marginatus in germany. | the ixodid tick dermacentor marginatus (sulzer, 1776) is endemic throughout southern europe in the range of 33-51 (°) n latitude. in germany, however, d. marginatus was exclusively reported in the rhine valley and adjacent areas. its northern distribution limit near giessen is located at the coordinates 8.32 (°) e/50.65 (°) n. particularly with regard to the causative agents of rickettsioses, tularemia, and q fever, the observed locations as well as the potential distribution of the vector d. ma ... | 2016 | 26993325 |
| detection of coxiella burnetii in ambient air after a large q fever outbreak. | one of the largest q fever outbreaks ever occurred in the netherlands from 2007-2010, with 25 fatalities among 4,026 notified cases. airborne dispersion of coxiella burnetii was suspected but not studied extensively at the time. we investigated temporal and spatial variation of coxiella burnetii in ambient air at residential locations in the most affected area in the netherlands (the south-east), in the year immediately following the outbreak. one-week average ambient particulate matter < 10 μm ... | 2016 | 26991094 |
| risungbinella massiliensis sp. nov., a new member of thermoactinomycetaceae isolated from human gut. | a novel filamentous bacterium, designated gd1(t), was isolated from the gut microbiota of a 38-year-old male who suffered from a coxiella burnetii vascular for which he received multiple a broad-spectrum antibiotic cocktail at the time of the stool collection. the strain was isolated as a part of culturomics study by cultivation on 5 % sheep blood agar in aerobic condition at 28 °c, after 14 days of incubation. strain gd1(t) shows 16s rrna gene sequence similarities of 98.01 % to the type strain ... | 2016 | 26984352 |
| modifications in the glycerophospholipid composition between the coxiella burnetii phase i and phase ii cells suggest an association with phase variation of the bacterium. | glycerophospholipids (gp) extracted from the coxiella burnetii strain nine mile in virulent phase i (nm i) and low virulent phase ii (nm ii) were analyzed by fourier transform ion cyclotron resonance (ft-icr) mass spectrometry (ms) that gave a superior mass resolution and mass accuracy allowing unambiguous peak recognition and precise assignment of ions. we showed that gp present in the pathogen's outer membrane underwent considerable modifications during the phase variation that might be relate ... | 2016 | 26982464 |
| complementation of arginine auxotrophy for genetic transformation of coxiella burnetii by use of a defined axenic medium. | host cell-free (axenic) culture of coxiella burnetii in acidified citrate cysteine medium-2 (accm-2) has provided important opportunities for investigating the biology of this naturally obligate intracellular pathogen and enabled the development of tools for genetic manipulation. however, accm-2 has complex nutrient sources that preclude a detailed study of nutritional factors required for c. burnetii growth. metabolic reconstruction of c. burnetii predicts that the bacterium cannot synthesize a ... | 2016 | 26969695 |
| treatment of an abdominal aortic aneurysm infected by coxiella burnetii using a cryopreserved allograft. | q fever is a worldwide zoonosis caused by an intracellular bacillus named coxiella burnetii (cb) and is a rare cause of vascular infections. we report a case of abdominal aortic aneurysm infected by cb with bilateral paravertebral abscesses and contiguous spondylodiscitis treated by open repair using a cryopreserved allograft and long-term antibiotic therapy by oral doxycycline and oral hydroxychloroquine for a duration of 18 months. twenty months after the operation, the patient had no infectio ... | 2016 | 26968369 |
| embolization of a large rapidly growing aortic pseudo-aneurysm not amenable to open or endovascular repair. | to report the case of a rapidly growing aortic false aneurysm because of q fever infection that was managed by embolization. | 2016 | 26968368 |
| coxiella burnetii seroprevalence and risk factors in cattle farmers and farm residents in three northeastern provinces and inner mongolia autonomous region, china. | little is known about coxiella burnetii infection among cattle farmers and farm residents in china. thus, the present study was conducted to detect the seroprevalence of c. burnetii infection and estimate associated risk factors among cattle farmers and farm residents in china. a cross-sectional study was designed, and sera of 362 people living or working on 106 cattle farms were tested for c. burnetii igg and igm antibodies by immunofluorescence assay. overall c. burnetii seroprevalence was 35. ... | 2016 | 26966688 |
| absence of serological evidence of rickettsia spp., bartonella spp., ehrlichia spp. and coxiella burnetii infections in american samoa. | little is known about the epidemiology of zoonotic diseases in american samoa (pacific). a review of literature did not identify any published information on human rickettsia spp., bartonella spp., ehrlichia spp. or coxiella burnetii infections in this country. to determine the presence of these diseases, we conducted a serosurvey of american samoans. the presence of immunoglobulin g antibodies against rickettsia felis, rickettsia typhi, rickettsia conorii, c. burnetii, bartonella henselae, bart ... | 2016 | 26965788 |
| large vessel vasculitis in a patient with acute q-fever: a case report. | q fever is a zoonosis caused by the rickettsial organism coxiella burnetii. infection has an acute course, usually with a self-limited febrile illness and the possibility of the evaluation to a chronic course with endocardial involvement. the presence of autoantibodies and various autoimmune disorders have also been associated with c. burnetii infection. we report a case of acute q fever in which the patient developed large vessel vasculitis. the fdg-pet/ct scan detected inflammation of the thor ... | 2014 | 26952153 |
| molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs. | an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ... | 2016 | 26943995 |
| mixed methods survey of zoonotic disease awareness and practice among animal and human healthcare providers in moshi, tanzania. | zoonoses are common causes of human and livestock illness in tanzania. previous studies have shown that brucellosis, leptospirosis, and q fever account for a large proportion of human febrile illness in northern tanzania, yet they are infrequently diagnosed. we conducted this study to assess awareness and knowledge regarding selected zoonoses among healthcare providers in moshi, tanzania; to determine what diagnostic and treatment protocols are utilized; and obtain insights into contextual facto ... | 2016 | 26943334 |
| a fatal case of disseminated chronic q fever: a case report and brief review of the literature. | chronic q fever is a rare infection, which mainly manifests as endocarditis, infection of vascular prostheses or aortic aneurysms. we present the case of a 74-year-old immunocompromised man with a haematologically disseminated coxiella burnetii infection, which has never been reported before. | 2016 | 26940462 |
| development of liposomal ciprofloxacin to treat lung infections. | except for management of pseudomonas aeruginosa (pa) in cystic fibrosis, there are no approved inhaled antibiotic treatments for any other diseases or for infections from other pathogenic microorganisms such as tuberculosis, non-tuberculous mycobacteria, fungal infections or potential inhaled biowarfare agents including francisella tularensis, yersinia pestis and coxiella burnetii (which cause pneumonic tularemia, plague and q fever, respectively). delivery of an antibiotic formulation via the i ... | 2016 | 26938551 |
| molecular detection of rickettsia, anaplasma, coxiella and francisella bacteria in ticks collected from artiodactyla in thailand. | a total of 79 ticks collected from sambar deer (cervus unicolor), barking deer (muntiacus muntjak) and wild boar (sus scrofa) were examined by pcr for the presence of rickettsia, anaplasma, coxiella, and francisella bacteria. of the 79 ticks, 13% tested positive for rickettsia, 15% tested positive for anaplasma, 4% tested positive for coxiella, and 3% tested positive for francisella. interestingly, triple infection with anaplasma, rickettsia and francisella was determined in a dermacentor auratu ... | 2016 | 26934997 |
| molecular identification of q fever in patients with a suspected diagnosis of dengue in brazil in 2013-2014. | q fever is an important cause of undifferentiated fever that is rarely recognized or reported in brazil. the objective of this study was to look for the presence of coxiella burnetii during a dengue fever outbreak in the municipality of itaboraí, rio de janeiro, brazil, where this bacterium had previously infected humans and domesticated animals. blood samples from clinically suspected dengue fever patients were tested by polymerase chain reaction (pcr) for c. burnetii; the dna was detected in n ... | 2016 | 26928831 |
| [q fever : a rare differential diagnosis of granulomatous disease]. | q fever is a worldwide distributed zoonotic disease with a mostly benign course, which regularly reoccurs in germany. this report is about a patient with sporadic serologically proven q fever, which also showed typical histopathological findings with nonspecific granulomatous hepatitis, usually seen in acute disease. the bone marrow biopsy revealed so-called doughnut granulomas, which are not pathognomonic but a typical finding in q fever. this case report impressively underlines that the histom ... | 2016 | 26919849 |
| transcriptional profiling of coxiella burnetii reveals extensive cell wall remodeling in the small cell variant developmental form. | a hallmark of coxiella burnetii, the bacterial cause of human q fever, is a biphasic developmental cycle that generates biologically, ultrastructurally, and compositionally distinct large cell variant (lcv) and small cell variant (scv) forms. lcvs are replicating, exponential phase forms while scvs are non-replicating, stationary phase forms. the scv has several properties, such as a condensed nucleoid and an unusual cell envelope, suspected of conferring enhanced environmental stability. to ide ... | 2016 | 26909555 |
| highly sensitive loop-mediated isothermal amplification for the detection of leptospira. | leptospirosis is a worldwide zoonosis caused by an infection with the pathogenic species of leptospira. we have developed a loop-mediated isothermal amplification (lamp) assay to detect the dna of leptospira spp. six sets of primers targeting the gene of the subsurface protein, lipl32, were evaluated for their detection sensitivity. the best primer set detected less than 25 copies of lipl32 per reaction of both plasmid dna template and purified leptospiral genomic dna. by combining primers targe ... | 2015 | 26904743 |
| detection of q fever specific antibodies using recombinant antigen in elisa with peroxidase based signal amplification. | currently, the accepted method for q fever serodiagnosis is indirect immunofluorescent antibody assay (ifa) using the whole cell antigen. in this study, we prepared the recombinant antigen of the 27-kda outer membrane protein (com1) which has been shown to be recognized by q fever patient sera. the performance of recombinant com1 was evaluated in elisa by ifa confirmed serum samples. due to the low titers of igg and igm in q fever patients, the standard elisa signals were further amplified by us ... | 2014 | 26904739 |
| development of an ex vivo tissue platform to study the human lung response to coxiella burnetii. | coxiella burnetii is an intracellular bacterial pathogen that causes human q fever, an acute debilitating flu-like illness that can also present as chronic endocarditis. disease typically occurs following inhalation of contaminated aerosols, resulting in an initial pulmonary infection. in human cells, c. burnetii generates a replication niche termed the parasitophorous vacuole (pv) by directing fusion with autophagosomes and lysosomes. c. burnetii requires this lysosomal environment for replicat ... | 2016 | 26902725 |
| epidemiology of brucellosis, q fever and rift valley fever at the human and livestock interface in northern côte d'ivoire. | northern côte d'ivoire is the main livestock breeding zone and has the highest livestock cross-border movements in côte d'ivoire. the aim of this study was to provide updated epidemiological data on three neglected zoonotic diseases, namely brucellosis, q fever and rift valley fever (rvf). we conducted three-stage cross-sectional cluster surveys in livestock and humans between 2012 and 2014 in a random selection of 63 villages and a sample of 633 cattle, 622 small ruminants and 88 people. we adm ... | 2017 | 26899680 |
| a cross sectional study evaluating the prevalence of coxiella burnetii, potential risk factors for infection, and agreement between diagnostic methods in goats in indiana. | coxiella burnetii is the etiologic agent of the zoonotic disease q fever and is considered to be endemic in domestic ruminants. small ruminants in particular are important reservoirs for human infection. serologic and molecular methods are both available for diagnosis of infection with c. burnetii, but there has been little research evaluating the prevalence of this organism in small ruminants outside of the context of clinical disease outbreaks. the objectives of this study were to estimate ser ... | 2016 | 26897246 |
| q fever. | q fever is a significant infectious disease threat to us military personnel deployed in the middle east. its environmental stability, aerosol transmission, and animal reservoir make it a considerable risk for deployed troops due to its potential for weaponization and risk of natural infection. it presents as a flu-like illness that responds promptly to antimicrobial therapy. q fever should be suspected in patients presenting with a compatible febrile illness in an endemic area and especially if ... | 2016 | 26874100 |
| first molecular evidence of coxiella burnetii in patients with atypical pneumonia, india. | 2016 | 26860433 | |
| the contribution of genomics to the study of q fever. | coxiella burnetii is the etiological agent of q fever, a worldwide zoonosis that can result in large outbreaks. the birth of genomics and sequencing of c. burnetii strains has revolutionized many fields of study of this infection. accurate genotyping methods and comparative genomic analysis have enabled description of the diversity of strains around the world and their link with pathogenicity. genomics has also permitted the development of qpcr tools and axenic culture medium, facilitating the d ... | 2016 | 26854360 |
| the key role of experiential uncertainty when dealing with risks: its relationships with demand for regulation and institutional trust. | the results of a survey and an experiment show that experiential uncertainty-people's experience of uncertainty in risk contexts-plays a moderating role in individuals' risk-related demand for government regulation and trust in risk-managing government institutions. first, descriptions of risks were presented to respondents in a survey (n = 1,017) and their reactions to questions about experiential uncertainty, risk perception, and demand for government regulation were measured, as well as level ... | 2016 | 26849482 |
| modulation of the host transcriptome by coxiella burnetii nuclear effector cbu1314. | coxiella burnetii is a gram-negative, obligate intracellular pathogen that directs the formation of a parasitophorous vacuole derived from the host lysosomal network. biogenesis and maintenance of this replicative compartment is dependent on bacterial protein synthesis and results in differential expression of specific host genes. however, the mechanisms by which the pathogen induces changes in the host transcriptome is poorly understood. in the current study we identified a dot/icm secreted eff ... | 2016 | 26827929 |
| [description of a case of severe q fever]. | the paper considers a rare clinical case of severe q fever in a young man with no compromised premorbid background. it describes and analyzes clinical manifestations and laboratory findings with consideration for the current data available in the literature. the issues of the differential diagnosis, laboratory diagnosis, and treatment of q fever are discussed. | 2015 | 26821422 |