Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| anaplasma phagocytophilum apta modulates erk1/2 signalling. | anaplasma phagocytophilum causes human granulocytic anaplasmosis, one of the most common tick-borne diseases in north america. this unusual obligate intracellular pathogen selectively persists within polymorphonuclear leucocytes. in this study, using the yeast surrogate model we identified an a. phagocytophilum virulence protein, apta (a. phagocytophilum toxin a), that activates mammalian erk1/2 mitogen-activated protein kinase. this activation is important for a. phagocytophilum survival within ... | 2011 | 20716207 |
| associations between coinfection prevalence of borrelia lusitaniae, anaplasma sp., and rickettsia sp. in hard ticks feeding on reptile hosts. | an increasing number of studies reveal that ticks and their hosts are infected with multiple pathogens, suggesting that coinfection might be frequent for both vectors and wild reservoir hosts. whereas the examination of associations between coinfecting pathogen agents in natural host-vector-pathogen systems is a prerequisite for a better understanding of disease maintenance and transmission, the associations between pathogens within vectors or hosts are seldom explicitly examined. we examined th ... | 2011 | 20711724 |
| application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples. | abstract: | 2010 | 20704695 |
| pyrosequencing and characterization of immune response genes from the american dog tick, dermacentor variabilis (l.). | ticks continue to be a threat to animal and human health, and new and novel control strategies are needed for ticks and tick-borne pathogens. the characterization of the tick-pathogen interface and the tick immune response to microbial infections is fundamental toward the formulation of new control strategies for ticks and the pathogens they transmit. our overall hypothesis for this research is that the tick immune system manages the maintenance of pathogens. therefore, discovery of tick immune ... | 2010 | 20698900 |
| effectiveness of rapid diagnostic tests to assess pathogens of fishers (martes pennanti) and gray foxes (urocyon cinereoargenteus). | wildlife managers often need to assess the current health status of wildlife communities before implementation of management actions involving surveillance, reintroductions, or translocations. we estimated the sensitivity and specificity of a commercially available domestic canine rapid diagnostic antigen test for canine parvovirus and a rapid enzyme-linked immunosorbent assay for the detection of antibodies toward anaplasma phagocytophilum on populations of fishers (martes pennanti) and sympatr ... | 2010 | 20688707 |
| the dusky-footed woodrat (neotoma fuscipes) is susceptible to infection by anaplasma phagocytophilum originating from woodrats, horses, and dogs. | the dusky-footed woodrat (neotoma fuscipes) is the putative reservoir for anaplasma phagocytophilum in the western united states based on high prevalence of a. phagocytophilum genetic material detected by polymerase chain reaction (pcr), high antibody prevalence, and infestation with the vector tick ixodes pacificus. following inoculation of nine wild-caught woodrats with woodrat-, canine-, or equine-origin a. phagocytophilum, all became pcr-positive and seroconverted. however, when pcr-positive ... | 2010 | 20688687 |
| experimental infection in lambs with a red deer (cervus elaphus) isolate of anaplasma phagocytophilum. | anaplasma phagocytophilum transmitted by ixodes spp. ticks is the causative agent of tick-borne fever (tbf) in domestic ruminants. tbf is widespread along the coast of southern norway and may cause a severe problem for the sheep industry. red deer (cervus elaphus) are important hosts for ticks and have been found to be infected naturally with a. phagocytophilum. however, it is unclear whether red deer could serve as reservoir hosts for a. phagocytophilum infections in sheep. we infected lambs ex ... | 2010 | 20688686 |
| seasonal prevalence of serum antibodies to whole cell and recombinant antigens of borrelia burgdorferi and anaplasma phagocytophilum in white-tailed deer in connecticut. | whole-blood samples were obtained from 214 white-tailed deer (odocoileus virginianus) representing 44 sites in connecticut (usa) during 1992, 1993, 1996, 1999, and 2000 through 2006. sera were analyzed for total antibodies to whole-cell or recombinant antigens of borrelia burgdorferi sensu stricto and anaplasma phagocytophilum, the respective causative agents of lyme borreliosis and human granulocytic anaplasmosis. deer sera contained antibodies to both bacteria during different seasons and thro ... | 2010 | 20688684 |
| detecting and differentiating theileria sergenti and theileria sinensis in cattle and yaks by pcr based on major piroplasm surface protein (mpsp). | theileria sergenti and theileria sinensis are closely related members of benign theileria species found in cattle and yaks in china. they are morphologically indistinguishable. a polymerase chain reaction (pcr) targeting major piroplasm surface protein of t. sergenti and t. sinensis was developed in this study. the newly developed oligonucleotide primer set was able to specifically amplify the dna of t. sinensis and in conjunction with primers for t. sergenti and these two species could be detec ... | 2010 | 20685208 |
| detection and prevalence of anaplasma phagocytophilum and rickettsia helvetica in ixodes ricinus ticks in seven study areas in sweden. | abstract: | 2010 | 20684755 |
| first case of human granulocytic anaplasmosis from slovakia. | in order to evaluate the potential risks of human granulocytic anaplasmosis in slovakia, blood and serum samples of hunters and foresters from the northern parth of slovakia were tested. we present the first case of hga from slovakia confirmed by nested pcr amplification of the 16s rrna gene fragment of a. phagocytophilum. | 2010 | 20684497 |
| molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan recife (north-eastern brazil). | to identify dna of the main tick-borne pathogens in dogs from recife (brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the veterinary hospital of the universidade federal rural de pernambuco from march 2007 to june 2008. the detection of dna was performed using specific primers. amplicons were analyzed through electrophoresis and sequencing. a phylogenetic tree was constructed using the upgma method, revealing that the sequences were closely related to tho ... | 2010 | 20680344 |
| development and laboratory evaluation of a lateral flow device (lfd) for the serodiagnosis of theileria annulata infection. | several dna-based and serological tests have been established for the detection of theileria annulata infection, including polymerase chain reaction, reverse line blot and loop-mediated isothermal amplification, indirect enzyme-linked immunosorbent assay (elisa), and competitive elisa. in this study, we have applied knowledge from the development and application of a recombinant protein-based indirect elisa and competitive elisa to establish a rapid test for point-of-care diagnosis of t. annulat ... | 2010 | 20680339 |
| type iv secretion in the obligatory intracellular bacterium anaplasma phagocytophilum. | anaplasma phagocytophilum is an obligatory intracellular bacterium that infects neutrophils, the primary host defence cells. consequent effects of infection on host cells result in a potentially fatal systemic disease called human granulocytic anaplasmosis. despite ongoing reductive genome evolution and deletion of most genes for intermediary metabolism and amino acid biosynthesis, anaplasma has also experienced expansion of genes encoding several components of the type iv secretion (t4s) appara ... | 2010 | 20670295 |
| a novel ehrlichia genotype detected in naturally infected cattle in north america. | during a research investigation to determine if cattle from british columbia (bc), canada were infected with anaplasma marginale or other related rickettsial blood parasites, a novel ehrlichia genotype was revealed. blood from seven bc source cattle was bioassayed by intravenous inoculation into naïve splenectomised calves. additional splenectomised calves were used as uninoculated negative control or a. marginale-inoculated positive control. newly designed sets of primers specific for the msp5 ... | 2010 | 20663613 |
| anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in ixodes scapularis ticks. | anaplasma phagocytophilum, the agent of human anaplasmosis, persists in ticks and mammals. we show that a. phagocytophilum induces the phosphorylation of actin in an ixodes ricinus tick cell line and ixodes scapularis ticks, to alter the ratio of monomeric/filamentous (g/f) actin. a. phagocytophilum-induced actin phosphorylation was dependent on ixodes p21-activated kinase (ipak1)-mediated signaling. a. phagocytophilum stimulated ipak1 activity via the g protein-coupled receptor gbetagamma subun ... | 2010 | 20660616 |
| diagnosis of canine vector-borne diseases in young dogs: a longitudinal study. | canine vector-borne diseases (cvbds) pose a diagnostic challenge, particularly when a dog is coinfected with more than one pathogen. the purpose of this study was to generate information about the diagnosis of cvbds in young dogs following their first exposure to flea, tick, sand fly, louse, and mosquito vectors. from march 2008 to may 2009, 10 purpose-bred young naive beagle dogs and a cohort of 48 mixed-breed dogs living in an area to which cvbd is endemic in southern italy were monitored usin ... | 2010 | 20660218 |
| molecular detection of ehrlichia canis, anaplasma bovis, anaplasma platys, candidatus midichloria mitochondrii and babesia canis vogeli in ticks from israel. | : ticks are vectors of important pathogens of human and animals. therefore, their microbial carriage capacity is constantly being investigated. the aim of this study was to characterize the diversity of domestic animal pathogens in ticks collected from vegetation and the ground, from different parts of israel. non-engorged questing adult ticks were collected from 13 localities. a total of 1196 ticks in 131 pools-83 pools of rhipicephalus turanicus and 48 of rhipicephalus sanguineus (with two to ... | 2011 | 20636417 |
| survival rates of immature ixodes pacificus (acari: ixodidae) ticks estimated using field-placed enclosures. | granulocytic anaplasmosis (ga) and lyme borreliosis are emerging tick-borne diseases caused by infection with anaplasma phagocytophilum and borrelia burgdorferi, respectively, and maintained in rodent-ixodes spp. tick cycles, including i. pacificus in the western u.s. ixodes pacificus has a multiple-year life cycle and b. burgdorferi and a. phagocytophilum are transstadially, but not transovarially, transmitted within ticks, thus ticks function importantly in maintaining infection in nature. in ... | 2010 | 20618646 |
| anaplasma phagocytophilum aph_0032 is expressed late during infection and localizes to the pathogen-occupied vacuolar membrane. | anaplasma phagocytophilum infects neutrophils and myeloid, endothelial, and tick cell lines to reside within a host cell-derived vacuole that is indispensible for its survival. here, we identify aph_0032 as an anaplasma-derived protein that associates with the a. phagocytophilum-occupied vacuolar membrane (avm). aph_0032 is a 66.1 kda acidic protein that electrophoretically migrates with an apparent molecular weight of 130 kda. it contains a predicted transmembrane domain and tandemly arranged d ... | 2010 | 20600793 |
| prevention of endemic canine vector-borne diseases using imidacloprid 10% and permethrin 50% in young dogs: a longitudinal field study. | canine vector-borne diseases (cvbds) are highly prevalent and increasing in distribution worldwide. a longitudinal study was conducted in southern italy to determine the incidence of and protection against cvbd-causing pathogens in dogs treated with a combination of imidacloprid 10% and permethrin 50% (imper). one hundred eleven autochthonous young dogs were divided into group a (n=63) and group b (n=48), both groups containing dogs positive and negative for one or more cvbd-causing pathogens. a ... | 2010 | 20591573 |
| haemorrhagic diathesis in a ram with anaplasma phagocytophilum infection. | a 10-month-old ram with fever, inappetence and haemorrhagic diathesis had petechiae and ecchymoses at various body sites and was infested by ticks. haematological examination revealed pancytopenia, while serum biochemistry indicated hepatic dysfunction. blood smears were negative for ehrlichia spp. and other haemoparasites. paired sera revealed infection by anaplasma phagocytophilum, but testing by polymerase chain reaction was negative. treatment with oxytetracycline was effective. this is the ... | 2011 | 20591440 |
| detection of anaplasma phagocytophilum in wild boar in slovenia. | 2009 | 20584165 | |
| anaplasma phagocytophilum propagation is enhanced in human complement-containing medium. | 2009 | 20584164 | |
| a 4-year study of anaplasma phagocytophilum in portugal. | 2009 | 20584163 | |
| the prevalence of anaplasma phagocytophilum in host-seeking ixodes ricinus ticks in norway. | 2009 | 20584162 | |
| first evidence of anaplasma infection in crete, greece. report of six human cases. | 2009 | 20584160 | |
| a morphological and molecular study of anaplasma phagocytophilum transmission events at the time of ixodes ricinus tick bite. | anaplasma phagocytophilum is the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever (tbf) in ruminants. the bacterium invades and replicates in phagocytes, especially in polymorphonuclear granulocytes. | 2010 | 20565721 |
| prevalence of anaplasma phagocytophilum in fallow deer (dama dama) and feeding ticks from an italy preserve. | up to date, information concerning the anaplasma phagocytophilum infection in fallow deer is scant, therefore, to verify its prevalence in these ungulates serological and pcr screenings were performed on blood of 72 fallow deer hunted in a central-northern italian preserve. molecular analyses were also performed on 90 ticks removed from the animals. a. phagocytophilum infection in fallow deer was confirmed in 20 out 72 by ifa assay and in 11 out 72 by pcr. the sequence obtained revealed a comple ... | 2011 | 20561656 |
| anaplasma phagocytophilum in livestock and small rodents. | to identify the prevalence of anaplasma phagocytophilum in both wild rodents and domestic animals and to make clear the genetic characteristics of the agents from different animals in china, a total of 105 livestock and 159 small rodents were analyzed by real-time-pcr and sequence analysis. the prevalence rate was 6.7% (7/105) and 14.5% (23/159), respectively. the nucleotide sequences of 16s rrna (rrs) from the positive livestock and rodents were identical to each other. the phylogenetic analysi ... | 2010 | 20558015 |
| ehrlichia ewingii infection and exposure rates in dogs from the southcentral united states. | we used pcr and a novel serologic assay to determine infection and exposure rates to ehrlichia ewingii in dogs from an area of northeast oklahoma and northwest arkansas where amblyomma americanum ticks are abundant. of 143 dogs assayed, 13 (9.1%) harbored e. ewingii by pcr and 64 (44.8%) had antibodies to e. ewingii detected using a peptide-based microtiter plate elisa. dogs were more likely (p=0.001) to be positive by pcr if sampled in august (30.8%) but no association was found between seropos ... | 2010 | 20541322 |
| prevalence of tick-borne pathogens in ticks in sicily. | the prevalence of anaplasma, ehrlichia, rickettsia and babesia/theileria species was analysed in questing and feeding adult ticks in sicily. a total of 678 ticks were collected and analysed in this study. of these, 29 were questing ticks and 649 were collected from infested cattle, sheep, goats or dogs. tick species analysed included rhipicephalus bursa, r. turanicus, r. sanguineus, hyalomma lusitanicum, h. marginatum, dermacentor marginatus, ixodes ricinus, r. (boophilus) annulatus and haemaphy ... | 2010 | 20537102 |
| phylogenetics and population genetics of the louse fly, lipoptena mazamae, from arkansas, usa. | louse flies, also known as deer keds (lipoptena mazamae rondani), infest cervids such as white-tailed deer, odocoileus virginianus and vector pathogens such as anaplasma and bartonella schoenbuchensis to cattle and humans, respectively. the population genetic structure of 30 l. mazamae collected from white-tailed deer in four regions of arkansas, u.s.a., designated by county boundaries, was examined using dna sequences of a 259-bp region of the mitochondrial dna rrna 16s gene. of the 259 nucleot ... | 2010 | 20534010 |
| prevalence of bartonella species, rickettsia felis, haemoplasmas and the ehrlichia group in the blood of cats and fleas in eastern australia. | to define the prevalence of bartonella spp., rickettsia felis, mycoplasma haemofelis, 'candidatus mycoplasma haemominutum' (mhm) and 'candidatus mycoplasma turicensis' (mtc) in cats and their fleas in eastern australia. | 2010 | 20529020 |
| anaplasma spp. in wild mammals and ixodes ricinus from the north of spain. | our objectives were to investigate the presence of anaplasma spp. infection in red deer, wild boars, and ixodes ricinus removed from deer surveyed in la rioja, as well as to analyze the presence of anaplasma spp. in i. ricinus from different spanish regions--ours included. a total of 21 deer and 13 wild boar blood samples as well as 295 i. ricinus removed from deer, vegetation, or asymptomatic people were tested by polymerase chain reaction targeting anaplasma spp. 16s rrna gene and groesl heat ... | 2011 | 20528172 |
| the development of a qpcr assay to detect tick (ixodida) dna and its implementation for the study of tick-borne pathogen transmission. | polymerase chain reaction (pcr) analysis is regularly used to detect pathogens within arthropod vectors, but has also been applied to investigate vector dna. this study details a novel highly sensitive quantitative pcr (qpcr) which detects and quantifies dna from ixodes ricinus, the european vector of anaplasma phagocytophilum. by pairing this with a qpcr to detect a. phagocytophilum, valid comparisons of pathogen load can be made between different sized tick-tissue samples. these qpcrs were val ... | 2010 | 20515685 |
| human ehrlichiosis and anaplasmosis. | human ehrlichiosis and anaplasmosis are acute febrile tick-borne diseases caused by various members of the genera ehrlichia and anaplasma (anaplasmataceae). human monocytotropic ehrlichiosis has become one of the most prevalent life-threatening tick-borne disease in the united states. ehrlichiosis and anaplasmosis are becoming more frequently diagnosed as the cause of human infections, as animal reservoirs and tick vectors have increased in number and humans have inhabited areas where reservoir ... | 2010 | 20513551 |
| human anaplasmosis and anaplasma ovis variant. | 2010 | 20507768 | |
| equine granulocytic anaplasmosis in the czech republic. | twelve confirmed cases of equine granulocytic anaplasmosis (ega) and five additional suspected cases, showing a compatible clinical history and specific igg titres of 1280 or above, were recorded in the czech republic during the period 2002 to 2008. the diagnosis was based on clinical signs, the detection of anaplasma phagocytophilum morulae in neutrophils in blood smears, serology and molecular methods. pyrexia (39.8 to 41.3 degrees c), depression, partial or total anorexia, limb oedema and ict ... | 2010 | 20495165 |
| prevalence of selected infectious agents in cats in ireland. | vector-borne bacterial and rickettsial agents and toxoplasma gondii, are common organisms in cats. some are potentially zoonotic or may be transmitted via blood transfusion. the current study investigated the prevalence of these agents in cats from dublin, ireland, for which no published data exists. whole blood (n=116) and sera (n=83) samples were obtained from 121 cats. dna was extracted from blood and assayed using polymerase chain reaction techniques for anaplasma species, bartonella species ... | 2010 | 20472484 |
| role of sand lizards in the ecology of lyme and other tick-borne diseases in the netherlands. | abstract: | 2010 | 20470386 |
| a comparison of six primer sets for detection of trypanosoma evansi by polymerase chain reaction in rodents and thai livestock. | to face the worldwide threat of surra caused by trypanosoma evansi, international organizations have stressed the need to evaluate and standardize diagnostic tools. pcr detection of t. evansi has known a great expansion during the last 20 years, but primer sets are often insufficiently assessed and compared. in this work, we compared the performances of six primer pairs-tbr1/2 (masiga et al., 1992), esag6/7 (holland et al., 2001a, b), tepan1/2 (panyim et al., 1993), pmutec f/r (wuyts et al., 199 ... | 2010 | 20466489 |
| detection of anaplasma marginale and a. phagocytophilum in bovine peripheral blood samples by duplex real-time reverse transcriptase pcr assay. | insufficient diagnostic sensitivity and specificity coupled with the potential for cross-reactivity among closely related anaplasma species has made the accurate determination of infection status problematic. a method for the development of simplex and duplex real-time quantitative reverse transcriptase pcr (qrt-pcr) assays for the detection of a. marginale and a. phagocytophilum 16s rrna in plasma-free bovine peripheral blood samples is described. the duplex assay was able to detect as few as 1 ... | 2010 | 20463162 |
| potential infectivity of anaplasma phagocytophilum strains in ixodes ricinus ticks from serbia. | anaplasma phagocytophilum strains from different geographical regions are characterised by diverse potential infectivity for humans and domesticated animals. we investigated the potential pathogenicity of a. phagocytophilum strains in ixodes ricinus ticks from 11 geographically different localities in serbia. sequences obtained in this study showed a high variability of p44 paralogues. some of them, however, formed groups with similarities greater than 86% ('similarity groups'). previous studies ... | 2010 | 20460222 |
| ecological factors characterizing the prevalence of bacterial tick-borne pathogens in ixodes ricinus ticks in pastures and woodlands. | ecological changes are recognized as an important driver behind the emergence of infectious diseases. the prevalence of infection in ticks depends upon ecological factors that are rarely taken into account simultaneously. our objective was to investigate the influences of forest fragmentation, vegetation, adult tick hosts, and habitat on the infection prevalence of three tick-borne bacteria, borrelia burgdorferi sensu lato, anaplasma phagocytophilum, and rickettsia sp. of the spotted fever group ... | 2010 | 20453131 |
| tickborne powassan virus infections among wisconsin residents. | powassan virus (powv) is a tickborne flavivirus that causes a rare but potentially life-threatening illness. the first reported case of powv infection in a wisconsin resident occurred in 2003. enhanced surveillance and testing detected 2 additional cases. | 2010 | 20443328 |
| identification of anaplasma marginale proteins specifically upregulated during colonization of the tick vector. | the transition between infection of the mammalian host and colonization of an arthropod vector is required for the ongoing transmission of a broad array of pathogens, from viruses to protozoa. understanding how this transition is mediated provides opportunities to disrupt transmission through either chemotherapy or immunization. we used an unbiased proteomic screen to identify anaplasma marginale proteins specifically upregulated in the tick compared to the mammalian host. comparative mass spect ... | 2010 | 20439479 |
| estimating seroprevalence and variation to four tick-borne infections and determination of associated risk factors in cattle under traditional mixed farming system in mbeere district, kenya. | a cross-sectional study of serum antibody responses of cattle to tick-borne disease (tbd) parasites (theileria parva, theileria mutans, anaplasma marginale and babesia bigemina) was conducted on traditional smallholder mixed farms in mbeere district in kenya. the objective was to estimate the infections' seroprevalence and variation and identify associated risk factors. a total of 440 cattle in 80 farms, selected by stratified random sampling from the four divisions in the district, were surveye ... | 2010 | 20434227 |
| molecular detection of anaplasma phagocytophilum in the european hedgehog (erinaceus europaeus) and its ticks. | the european hedgehog (erinaceus europaeus) is a common wild mammal in central europe that shares habitats with humans in urban, peri-urban, and rural areas. thus, this species may play a role in human contact with zoonotic diseases. here we report the presence of the pathogen anaplasma phagocytophilum in hedgehogs and their ticks in germany. | 2010 | 20426690 |
| serological study of selected vector-borne diseases in shelter dogs in central spain using point-of-care assays. | we evaluated the prevalence of selected vector-borne diseases in 131 dogs in an animal shelter in central spain using point-of-care assays (snap 4dx and snap leishmania; idexx laboratories, westbrook, me). the snap 4dx detects dirofilaria immitis (di) antigen and antibodies against ehrlichia canis (ec), borrelia burgdorferi (bb), and anaplasma phagocytophylum (aph); the snap leishmania kit detects antibodies against leishmania infantum (li). dogs were classified as healthy or sick based on physi ... | 2010 | 20420531 |
| anaplasma phagocytophilum from rodents and sheep, china. | to characterize the strains of anaplasma phagocytophilum in wild and domestic animals in china, we isolated the organism from rodents and sheep in northeastern china. we isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in balb/c mice and then cell culture in hl60 cells. the 3 isolates were identified by wright-giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16s rrna gene, partial citrate synthase gene, ... | 2010 | 20409364 |
| characterization of anaplasma phagocytophilum and a. ovis infection in a naturally infected sheep flock with poor health condition. | anaplasma species are transmitted by ticks and cause diseases in humans and animals. these pathogens infect sheep, an economically important domestic animal worldwide. the current study was designed to characterize in 200 animals the infection with anaplasma phagocytophilum and anaplasma ovis and the genetic diversity of a. ovis strains collected from a naturally infected sheep flock with poor health condition. sheep had 98% seroprevalence to anaplasma spp. antibodies. pcr results confirmed the ... | 2010 | 20405320 |
| variant -and individual dependent nature of persistent anaplasma phagocytophilum infection. | anaplasma phagocytophilum is the causative agent of tick-borne fever in ruminants and human granulocytotropic anaplasmosis (hga). the bacterium is able to survive for several months in immune-competent sheep by modifying important cellular and humoral defence mechanisms. little is known about how different strains of a. phagocytophilum propagate in their natural hosts during persistent infection. | 2010 | 20398321 |
| neutrophils exposed to a. phagocytophilum under shear stress fail to fully activate, polarize, and transmigrate across inflamed endothelium. | anaplasma phagocytophilum is an obligate intracellular bacterium that has evolved mechanisms to hijack polymorphonuclear neutrophil (pmn) receptors and signaling pathways to bind, infect, and multiply within the host cell. e-selectin is upregulated during inflammation and is a requisite endothelial receptor that supports pmn capture, rolling, and activation of integrin-mediated arrest. ligands expressed by pmn that mediate binding to endothelium via e-selectin include sialyl lewis x (sle(x))-exp ... | 2010 | 20392928 |
| imported and travelling dogs as carriers of canine vector-borne pathogens in germany. | abstract: | 2010 | 20377872 |
| experimental infection and co-infection of dogs with anaplasma platys and ehrlichia canis: hematologic, serologic and molecular findings. | abstract: | 2010 | 20377870 |
| comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in korea. | abstract: a serological survey for dirofilaria immitis, anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi infections in rural hunting and urban shelter dogs mainly from southwestern regions of the republic of korea (south korea) was conducted. from a total of 229 wild boar or pheasant hunting dogs, the number of serologically positive dogs for any of the four pathogens was 93 (40.6%). the highest prevalence observed was d. immitis (22.3%), followed by a. phagocytophilum (18.8% ... | 2010 | 20377869 |
| canine babesiosis in northern portugal and molecular characterization of vector-borne co-infections. | abstract: | 2010 | 20377861 |
| anaplasma phagocytophilum and ehrlichia chaffeensis: subversive manipulators of host cells. | anaplasma spp. and ehrlichia spp. cause several emerging human infectious diseases. anaplasma phagocytophilum and ehrlichia chaffeensis are transmitted between mammals by blood-sucking ticks and replicate inside mammalian white blood cells and tick salivary-gland and midgut cells. adaptation to a life in eukaryotic cells and transmission between hosts has been assisted by the deletion of many genes that are present in the genomes of free-living bacteria (including genes required for the biosynth ... | 2010 | 20372158 |
| the efficacy of three chlortetracycline regimens in the treatment of persistent anaplasma marginale infection. | chemosterilization is reported in cattle fed chlortetracycline hydrochloride (ctc) at dosages ranging from 1.1mg/kg for 120 days to 11 mg/kg for 30-60 days. the relationship between plasma ctc drug concentration and carrier clearance has not been described. chronic carrier status was established in 21 steers with a virginia isolate of anaplasma marginale and confirmed by celisa and an a. marginale-specific rt-pcr. four negative, splenectomized steers served as active disease transmission sentine ... | 2010 | 20346598 |
| the anaplasma phagocytophilum-occupied vacuole selectively recruits rab-gtpases that are predominantly associated with recycling endosomes. | anaplasma phagocytophilum is an obligate intracellular bacterium that infects neutrophils to reside within a host cell-derived vacuole. the a. phagocytophilum-occupied vacuole (apv) fails to mature along the endocytic pathway and is non-fusogenic with lysosomes. rab gtpases regulate membrane traffic. to better understand how the bacterium modulates the apv's selective fusogencity, we examined the intracellular localization of 20 green fluorescent protein (gfp) or red fluorescent protein (rfp)-ta ... | 2010 | 20345488 |
| fucosylation enhances colonization of ticks by anaplasma phagocytophilum. | fucosylated structures participate in a wide range of pathological processes in eukaryotes and prokaryotes. the impact of fucose on microbial pathogenesis, however, has been less appreciated in arthropods of medical relevance. thus, we used the tick-borne bacterium anaplasma phagocytophilum- the agent of human granulocytic anaplasmosis to understand these processes. here we show that a. phagocytophilum uses alpha1,3-fucose to colonize ticks. we demonstrate that a. phagocytophilum modulates the e ... | 2010 | 20331643 |
| association of pathogen strain-specific gene transcription and transmission efficiency phenotype of anaplasma marginale. | efficient transmission of pathogens by an arthropod vector is influenced by the ability of the pathogen to replicate and develop infectiousness within the arthropod host. while the basic life cycle of development within and transmission from the arthropod vector are known for many bacterial and protozoan pathogens, the determinants of transmission efficiency are largely unknown and represent a significant gap in our knowledge. the st. maries strain of anaplasma marginale is a high-transmission-e ... | 2010 | 20308303 |
| first described case of human granulocytic anaplasmosis in a patient in eastern austria. | a 64-year-old otherwise healthy patient presented with high fever, thrombocytopenia, elevated liver enzymes and an erythema on the belly. the patient remembered a tick bite four weeks ago when walking with his dog before the specific symptoms started. a meningococcal disease or hematological illness was excluded. the serological results for tick-borne diseases showed a high igg antibody titer for anaplasma phagocytophila. all symptoms and laboratory parameters normalized after one week of hospit ... | 2010 | 20300926 |
| differential expression of genes in salivary glands of male rhipicephalus (boophilus)microplus in response to infection with anaplasma marginale. | bovine anaplasmosis, caused by the rickettsial tick-borne pathogen anaplasma marginale (rickettsiales: anaplasmataceae), is vectored by rhipicephalus (boophilus)microplus in many tropical and subtropical regions of the world. a. marginale undergoes a complex developmental cycle in ticks which results in infection of salivary glands from where the pathogen is transmitted to cattle. in previous studies, we reported modification of gene expression in dermacentor variabilis and cultured ixodes scapu ... | 2010 | 20298599 |
| further observations on the nature of anaplasma. | 1946 | 20244143 | |
| prevalence and seasonality of tick-borne pathogens in questing ixodes ricinus ticks from luxembourg. | in europe, ixodid ticks are important arthropod vectors of human and animal pathogens, but comprehensive studies of the prevalence of all relevant pathogens in central europe are scarce. as a result of ecological changes, the incidences of tick-borne infections are expected to increase. in this study, 1,394 nymphal and adult ixodes ricinus ticks sampled monthly during the active season from 33 ecologically distinct collection sites throughout luxembourg were screened for all human tick-borne pat ... | 2010 | 20228110 |
| diagnostic accuracy of methods for detecting anaplasma marginale infection in lactating dairy cattle of puerto rico. | bovine anaplasmosis (ba) is a hemoparasitic disease of great importance in cattle within the tropical and subtropical regions of the world. control programs for ba require accurate diagnostic assays but validation can be challenging because the true disease status of all animals is frequently not known with certainty. the objective of this study was to estimate the accuracy of assays for detection of anaplasma marginale infection in lactating dairy cattle of puerto rico using bayesian methods wi ... | 2010 | 20224076 |
| phylogenetic analysis of the genus anaplasma in southwestern china based on 16s rrna sequence. | to identify the species within the genus anaplasma circulating among ruminants in the southwest of china, we performed the phylogenetic analysis of the 16s rrna gene of two anaplasma isolates from cattle and seven from goats. the two sequences obtained from cattle strains belonged to the a. marginale cluster, whereas the other seven sequences from caprine strains formed two anaplasma spp. clusters, which diverged earlier than the clusters of a. marginale, a. centrale and a. ovis. these results i ... | 2010 | 20219219 |
| anaplasma phagocytophilum aph_1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane. | obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. here we identified aph_1387 as the first a. phagocytophilum-derived protein that associates with the a. phagocytophilum- ... | 2010 | 20212090 |
| [effect of coinfections in ixodidae ticks on transmission of blood microparasites]. | the purpose of this review was to describe and discuss the current spectrum of coinfections in ixodidae ticks and their effects on the transmission of blood microparasites. coinfections with borrelia burgdorferi s. l. and anaplasma phagocytophilum and/or babesia sp. in ticks from poland appear to be common, however, the potential influence on transmission dynamics, the mechanism of genetic variation and the ecology of interactions between pathogens remain poorly understood compared with infectio ... | 2009 | 20209806 |
| an indirect elisa for detection of theileria sergenti antibodies in water buffalo using a recombinant major piroplasm surface protein. | in this study we investigated the prevalence and enzootic potential of theileria spp. in water buffalo in the hubei province in china. an indirect elisa based on a recombinant major piroplasma surface protein was developed. the complete orf of the 33-kda major piroplasma surface protein (p33) was obtained from theileria sergenti genomic dna by pcr, cloned into the pet-28(a) vector and expressed in e. coli as a his-fusion protein. then the recombinant p33 (rp33) was purified and used as the antig ... | 2010 | 20207484 |
| the increasing recognition of rickettsial pathogens in dogs and people. | dogs and people are exposed to and susceptible to infection by many of the same tick-borne bacterial pathogens in the order rickettsiales, including anaplasma phagocytophilum, ehrlichia canis, e. chaffeensis, e. ewingii, rickettsia rickettsii, r. conorii, and other spotted fever group rickettsiae. recent findings include descriptions of novel ehrlichia and rickettsia species, recognition of the occurrence and clinical significance of co-infection, and increasing awareness of rhipicephalus sangui ... | 2010 | 20207197 |
| potential for tick-borne bartonelloses. | as worldwide vectors of human infectious diseases, ticks are considered to be second only to mosquitoes. each tick species has preferred environmental conditions and biotopes that determine its geographic distribution, the pathogens it vectors, and the areas that pose risk for tick-borne diseases. researchers have identified an increasing number of bacterial pathogens that are transmitted by ticks, including anaplasma, borrelia, ehrlichia, and rickettsia spp. recent reports involving humans and ... | 2010 | 20202411 |
| the immunization-induced antibody response to the anaplasma marginale major surface protein 2 and its association with protective immunity. | many vector-borne pathogens evade clearance via rapid variation in their immunogenic surface expressed proteins. this is exemplified by anaplasma marginale, a tick-borne bacterial pathogen that generates major surface protein 2 (msp2) variants to provide for immune escape and allow long-term pathogen persistence. in contrast to persistence following infection, immunization with a surface protein complex, which includes msp2, induces a response that prevents infection upon challenge. we hypothesi ... | 2010 | 20199762 |
| [identification of anaplasma phagocytophilum in small mammals from hengduan mountains of southwest china.]. | to investigate the prevalence of anaplasma phagocytophilum in small mammals from the forest area of hengduan mountains in southwestern china. | 2009 | 20193314 |
| microchip electrophoretic separation for the fast diagnosis of anaplasma phagocytophilum infection in cattle. | we report a diagnostic method for anaplasma phagocytophilum (a. phagocytophilum) infection in cattle using a nested pcr and microchip electrophoresis (me). a. phagocytophilum causes human granulocytic anaplasmosis and granulocytic ehrlichiosis, which are emerging tick-borne zoonotic diseases. nested pcr was used to amplify genomic dna samples extracted from cattle blood. the amplified pcr products were analyzed under a sieving gel matrix of 0.7% poly(ethyleneoxide) (m(r)=8,000,000) in a conventi ... | 2010 | 20175090 |
| anaplasma phagocytophilum ats-1 is imported into host cell mitochondria and interferes with apoptosis induction. | anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, infects human neutrophils and inhibits mitochondria-mediated apoptosis. bacterial factors involved in this process are unknown. in the present study, we screened a genomic dna library of a. phagocytophilum for effectors of the type iv secretion system by a bacterial two-hybrid system, using a. phagocytophilum vird4 as bait. a hypothetical protein was identified as a putative effector, hereby named anaplasmatranslo ... | 2010 | 20174550 |
| in-vitro studies on the susceptibility of ovine strains of anaplasma phagocytophilum to antimicrobial agents and to immune serum. | five strains of anaplasma phagocytophilum, the causative agent of tick-borne fever of sheep and cattle and human granulocytic anaplasmosis, were tested in vitro for their susceptibility to nine antibiotics using the continuous tick cell line ise6. minimum inhibitory concentrations (mics) were evaluated by comparing the percentage of infection of giemsa-stained antibiotic-treated anaplasma phagocytophilum-infected cells with that of untreated controls after 6 days of culture. the minimum bacteric ... | 2010 | 20170925 |
| subolesin expression in response to pathogen infection in ticks. | ticks (acari: ixodidae) are vectors of pathogens worldwide that cause diseases in humans and animals. ticks and pathogens have co-evolved molecular mechanisms that contribute to their mutual development and survival. subolesin was discovered as a tick protective antigen and was subsequently shown to be similar in structure and function to akirins, an evolutionarily conserved group of proteins in insects and vertebrates that controls nf-kb-dependent and independent expression of innate immune res ... | 2010 | 20170494 |
| canine and feline vector-borne diseases in italy: current situation and perspectives. | in italy, dogs and cats are at risk of becoming infected by different vector-borne pathogens, including protozoa, bacteria, and helminths. ticks, fleas, phlebotomine sand flies, and mosquitoes are recognized vectors of pathogens affecting cats and dogs, some of which (e.g., anaplasma phagocytophilum, borrelia burgdorferi, dipylidium caninum, leishmania infantum, dirofilaria immitis, and dirofilaria repens) are of zoonotic concern. recent studies have highlighted the potential of fleas as vectors ... | 2010 | 20145730 |
| molecular detection of babesia spp. and other haemoparasitic infections of cattle in maputo province, mozambique. | molecular detection of babesia species in apparently healthy cattle within an endemic region was carried out in order to determine the prevalence of carriers and the geographical distribution of babesia bigemina and babesia bovis in maputo province, mozambique. samples from 477 animals at 5 localities were analysed using 2 techniques, the semi-nested hot-start pcr and the reverse line blot (rlb) assay. with the semi-nested hot-start pcr, detection of b. bigemina ranged between 30% and 89%, and o ... | 2010 | 20128941 |
| merozoite proteins from babesia sp. bq1 (lintan) as potential antigens for serodiagnosis by elisa. | babesia sp. bq1 (lintan) is a babesia isolated from sheep infested with haemaphysalis qinghaiensis in china, and is closely related to b. motasi based on the 18s rrna gene sequence. in the present study, an elisa was developed with merozoite antigens of babesia sp. bq1 (lintan) (bqma) purified from in vitro culture. when the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95.5%. except for babesia sp. tianzhu, there was no cross- ... | 2010 | 20109252 |
| evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for ehrlichia, anaplasma, spotted fever group rickettsia, bartonella, and borrelia species in canine neurological diseases (109 cases). | vector-transmitted microorganisms in the genera ehrlichia, anaplasma, rickettsia, bartonella, and borrelia are commonly suspected in dogs with meningoencephalomyelitis (mem), but the prevalence of these pathogens in brain tissue and cerebrospinal fluid (csf) of dogs with mem is unknown. | 2010 | 20102497 |
| coinfection of western gray squirrel (sciurus griseus) and other sciurid rodents with borrelia burgdorferi sensu stricto and anaplasma phagocytophilum in california. | overlapping geographic distributions of tick-borne disease agents utilizing the same tick vectors are common, and coinfection of humans, domestic animals, wildlife, and ticks with both borrelia burgdorferi and anaplasma phagocytophilum has been frequently reported. this study was undertaken in order to evaluate the prevalence of both b. burgdorferi sensu stricto (hereinafter referred to as b. burgdorferi) and a. phagocytophilum in several species of sciurid rodents from northern california, usa. ... | 2010 | 20090047 |
| surveys for disease agents in introduced elk in arkansas and kentucky. | surveys for disease agents were conducted in introduced free-ranging elk (cervus elaphus nelsoni) in arkansas and kentucky. elk had been captured in colorado and nebraska and released in arkansas during 1981-1985. from 1997 through 2002 elk were captured in arizona, kansas, north dakota, new mexico, oregon, and utah and released in southeastern kentucky. specimens were collected from 170 hunter-killed elk in arkansas during 1998-2006, and 44 elk in kentucky during 2001-2004. significant findings ... | 2010 | 20090032 |
| detection of anaplasma phagocytophilum in amblyomma flavomaculatum ticks (acari: ixodidae) collected from lizard varanus exanthematicus imported to poland. | adults and nymphs of amblyomma exornatum, a. flavomaculatum, a. latum, amblyomma spp. and hyalomma aegyptium, were collected from savannah monitors, royal python (africa, ghana) and marginated tortoises (europe, greece) imported to poland, in 2004-2007. altogether 345 ticks were examined by polymerase chain reaction for rickettsial agents. none of the tested ticks was positive for the glta gene of rickettsia spp. dna of 16s rrna gene from anaplasma phagocytophilum was amplified and sequenced fro ... | 2010 | 20084536 |
| seroprevalence of borrelia burgdorferi sensu lato and anaplasma phagocytophilum in danish horses. | borrelia burgdorferi sensu lato and anaplasma phagocytophilum are able to infect horses. however, the extend to which danish horses are infected and seroconvert due to these two bacteria is unknown. the aim of the present study was to evaluate the seroprevalence of b. burgdorferi sensu lato and a. phagocytophilum in danish horses. | 2010 | 20082693 |
| [ehrlichiosis/anaplasmosis]. | ehrlichiosis and anaplasmosis are zoonoses caused by bacteria from the family anaplasmataceae, including human and animal pathogens. the human pathogens are ehrlichia chaffeensis, the causative agent of human monocytic ehrlichiosis (hme), anaplasma phagocytophilum, the pathogen causing human granulocytic anaplasmosis (hga), e. ewingii and neorickettsia sennetsu, granulocytotropic and monocytotropic ehrlichia species, respectively. ehrlichia spp. are small, gram-negative, obligate intracellular b ... | 2009 | 20077398 |
| [transfer of exotic ticks (acari: ixodida) on reptiles (reptilia) imported to poland]. | in the of period 2003-2007, a total of 382 specimens of reptiles belonging to the following genera were investigated: testudo, iguana, varanus, gongylophis, python, spalerosophis, psammophis. the material for the present study was a collection of reptiles owned by the "animals" ltd from swietochłowice (upper silesia, poland), specialising in import of exotic animals to poland, as well as the reptile collections of private breeders. the reptiles that turned out to be the most heavily infected wit ... | 2009 | 20066784 |
| anaplasma marginale type iv secretion system proteins virb2, virb7, virb11, and vird4 are immunogenic components of a protective bacterial membrane vaccine. | anaplasma and related ehrlichia spp. are important tick-borne, gram-negative bacterial pathogens of livestock and humans that cause acute infection and disease and can persist. immunization of cattle with an anaplasma marginale fraction enriched in outer membranes (om) can provide complete protection against disease and persistent infection. serological responses of om vaccinees to the om proteome previously identified over 20 antigenic proteins, including three type iv secretion system (t4ss) p ... | 2010 | 20065028 |
| [effects of the oxitetracycline in the increase of the packed cell volume of cows milk jugs during outbreak of bovine anaplasmosis]. | this research was performed to study the effects of oxytetracicline l.a., in unique dose, in the reconstitution of the packed cell volume (pcv) from animals clinically affected by anaplasmosis. were utilized 152 holstein frisian cows, of first lactation with age group varying between three and four years old. the animals were classified according to the presence of anaplasma marginale in the circulation and percentage of pcv as: chronic infection (pcve24%) and clinical (pcv<23%) constituting gro ... | 2008 | 20059824 |
| [seasonal dynamics of amblyomma ticks (acari:ixodidae) in an urban park of londrina city, parana, brazil]. | ticks are important to public health due to diseases they transmit to animals and humans and, economic losses they cause to livestock production. among other agents, such as babesia, ehrlichia, anaplasma, the rickettsia are the most important pathogens transmitted by ticks in brazil. worldwide there are about 870 tick species described and, of these, 55 species were already reported in brazil, being the genus amblyomma the most numerous with 33 species. the a. cajennense is the principal tick in ... | 2008 | 20059815 |
| [cross-reaction evaluation of pcr-anaplasma platys positive dogs tested to anaplasma phagocytophilum antibodies by commercial elisa]. | anaplasma platys, agent of canine cyclic thrombocytopenia parasites exclusively dogs platelets. its probable vector is the tick rhipicephalus sanguineus. among the existent diagnostic methods, the most used include: morulae identification in blood smears; antibody detection by indirect immunofluorescence; or dna amplification by polymerase chain reaction (pcr). recently a new commercial elisa (idexx®), capable of detecting a. phagocytophilum antibodies, has been developed. according to the manuf ... | 2008 | 20059806 |
| anaplasma phagocytophilum and ehrlichia chaffeensis type iv secretion and ank proteins. | the obligatory intracellular bacterial pathogens anaplasma and ehrlichia infect leukocytes by hijacking host-cell components and processes. the type iv secretion system is up-regulated during infection. among type iv secretion candidate substrates, an ankyrin repeat protein of anaplasma phagocytophilum, anka, is delivered into the host cytoplasm via a complex that includes vird4. anka is highly tyrosine phosphorylated and binds to the abl interactor 1, shp-1, and nuclear dna fragments. ehrlichia ... | 2010 | 20053580 |
| anaplasma phagocytophilum infection of red foxes (vulpes vulpes). | the investigation of anaplasma phagocytophilum infection covered 111 red foxes from the mazovian province. determination was based on pcr amplification of 16s rrna gene and the portion of msp4 gene coding msp4 major surface protein. the presence of a. phagocytophilum was found in 2.7 % of foxes. | 2009 | 20047265 |
| occurrence of igg antibodies to anaplasma phagocytophilum in humans suspected of lyme borreliosis in eastern slovakia. | human granulocytic anaplasmosis (hga) and lyme borreliosis (lb) are tick-borne and emerging infectious diseases caused by the anaplasma phagocytophilum and borrelia burgdorferi species. in europe, including slovakia, the principal vector of both pathogens is the common tick - ixodes ricinus, in which double infections with these pathogens have been reported. the aim of our study was evidence of igg antibodies against a. phagocytophilum in blood sera of humans with suspects lb from several clinic ... | 2009 | 20047263 |
| phylogenetic analysis of the 16s rrna gene of anaplasma species detected from japanese serows (capricornis crispus). | nineteen blood samples collected from free-living japanese serows, capricornis crispus, between 2006 and 2008 in iwate prefecture were examined for the anaplasma infection by pcr amplification of a part of the 16s rrna gene. ten (52.6%) out of the 19 samples produced a visible band in electrophoresed agarose gels. positive pcr products were subjected to dna sequencing. we found the nucleotide sequences were identical. almost entire length of the 16s rrna gene for a representative stain was then ... | 2009 | 20046041 |
| molecular diagnosis of anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis. | ehrlichioses are important emerging zoonotic tick-borne diseases that can affect both animals and humans. clinical manifestations of ehrlichiosis caused by different members of anaplasmataceae in dogs are similar to each other and to other diseases showing systemic manifestation. the observation of inclusions in white blood cells and in platelets cannot be used to confirm the anaplasmataceae etiologic agent of the disease. in this work we assessed the presence of anaplasmataceae agents in 51 dog ... | 2009 | 20040204 |
| [comparison of nested-pcr with blood smear examination in detection of ehrlichia canis and anaplasma platys in dogs]. | the clinical signs of ehrlichia canis and anaplasma platys infection are similar, and the diagnosis of these pathogens made by stained blood smears is poor due sensibility and specificity. on the other hand, the molecular diagnosis is highly sensitive and specific and nested-pcr have been optimized for accurate diagnosis these pathogens in dogs. at the veterinary teaching hospital, whole-blood samples with edta were obtained from 100 dogs and smears were made from blood samples for evaluation fo ... | 2009 | 20040193 |