Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| sequence analysis of insecticidal genes from xenorhabdus nematophilus pmfi296. | three strains of xenorhabdus nematophilus showed insecticidal activity when fed to pieris brassicae (cabbage white butterfly) larvae. from one of these strains (x. nematophilus pmfi296) a cosmid genome library was prepared in escherichia coli and screened for oral insecticidal activity. two overlapping cosmid clones were shown to encode insecticidal proteins, which had activity when expressed in e. coli (50% lethal concentration [lc(50)] of 2 to 6 microg of total protein/g of diet). the complete ... | 2001 | 11319082 |
| interaction of xenorhabdus nematophilus (enterobacteriaceae) with the antimicrobial defenses of the house cricket, acheta domesticus. | fifth instar acheta domesticus nymphs exhibited a decline in total hemocyte counts during the first hour of exposure to dead xenorhabdus nematophilus; the bacterial level in the hemolymph also declined during this time. thereafter bacterial numbers in the hemolymph increased as the level of damaged hemocytes increased. the bacteria lowered phenoloxidase activity in vivo by initially reducing the number of hemocytes containing prophenoloxidase and later by inhibiting enzyme activation. preincubat ... | 2000 | 11112374 |
| a new broad-spectrum protease inhibitor from the entomopathogenic bacterium photorhabdus luminescens. | a new protease inhibitor was purified to apparent homogeneity from a culture medium of photorhabdus luminescens by ammonium sulfate precipitation and preparative isoelectric focusing followed by affinity chromatography. ph. luminescens, a bacterium symbiotically associated with the insect-parasitic nematode heterorhabditis bacteriophora, exists in two morphologically distinguishable phases (primary and secondary). it appears that only the secondary-phase bacterium produces this protease inhibito ... | 2000 | 11101672 |
| novel insecticidal toxins from nematode-symbiotic bacteria. | the current strategy of using transgenic crops expressing insecticidal protein toxins is placing increasing emphasis on the discovery of novel toxins, beyond those already derived from the bacterium bacillus thuringiensis. here we review the cloning of four insecticidal toxin complex (tc) encoding genes from a different bacterium photorhabdus luminescens and of similar gene sequences from xenorhabdus nematophilus. both these bacteria occupy the gut of entomopathogenic nematodes and are released ... | 2000 | 10892346 |
| eicosanoids rescue spodoptera exigua infected with xenorhabdus nematophilus, the symbiotic bacteria to the entomopathogenic nematode steinernema carpocapsae. | xenorhabdus nematophilus is a pathogenic bacterium causing insect haemolymph septicemia, which leads to host insect death. to address the fundamental mechanisms underlying this haemolymph septicemia, or the immunodepressive response of the host insects following bacterial infection, we tested a hypothesis that the insect immune-mediating eicosanoid pathway is blocked by inhibitory action of the bacterium. haemocoelic injection of the bacteria into the fifth instar larvae of spodoptera exigua red ... | 2000 | 10891575 |
| inactivation of a novel gene produces a phenotypic variant cell and affects the symbiotic behavior of xenorhabdus nematophilus. | xenorhabdus nematophilus is an insect pathogen that lives in a symbiotic association with a specific entomopathogenic nematode. during prolonged culturing, variant cells arise that are deficient in numerous properties. to understand the genetic mechanism underlying variant cell formation, a transposon mutagenesis approach was taken. three phenotypically similar variant strains of x. nematophilus, each of which contained a single transposon insertion, were isolated. the insertions occurred at dif ... | 2000 | 10742251 |
| interaction of hemocytes and prophenoloxidase system of fifth instar nymphs of acheta domesticus with bacteria. | the hemocytes to which bacteria adhere were defined and the contribution of the prophenoloxidase system of fifth instar nymphs of acheta domesticus to adhesion were examined. the physicochemical parameters affecting hemocyte and phenoloxidase activity were determined. both plasmatocytes and granular cells responded to bacteria, the latter cells entrapping the microorganisms on filopodial extensions. the optimum ph for hemocyte adhesion to glass slides was 6.5, the granular cells being the most s ... | 2000 | 10736521 |
| pathogenicity, development, and reproduction of heterorhabditis bacteriophora and steinernema carpocapsae under axenic in vivo conditions. | galleria mellonella larvae cultured axenically were treated with axenic dauer juveniles of heterorhabditis bacteriophora and steinernema carpocapsae. after 3 days s. carpocapsae had killed all insects, with 9.4 +/- 4.3 nematodes per larva. h. bacteriophora were unable to kill g. mellonella, although 13.3 +/- 6.4 nematodes per galleria were found in the hemocoel. invading nematodes of both strains recovered from the dauer stage. h. bacteriophora developed into hermaphrodites with eggs and j1 in t ... | 2000 | 10631058 |
| pathogenicity caused by high virulent and low virulent strains of steinernema carpocapsae to galleria mellonella. | steinernema carpocapsae is an entomopathogenic nematode associated with a symbiotic bacterium, xenorhabdus nematophilus. both components of the complex participate in a pathogenic process in insects. this has raised two questions: how much does each one participate, and what mechanisms are involved? in this paper we compare the virulence of two strains of s. carpocapsae: a high virulent strain (breton) and a low virulent strain (az27), both of which are free of symbiotic bacteria. breton and az2 ... | 2000 | 10631057 |
| flhdc, the flagellar master operon of xenorhabdus nematophilus: requirement for motility, lipolysis, extracellular hemolysis, and full virulence in insects. | xenorhabdus is a major insect pathogen symbiotically associated with nematodes of the family steinernematidae. this motile bacterium displays swarming behavior on suitable media, but a spontaneous loss of motility is observed as part of a phenomenon designated phase variation which involves the loss of stationary-phase products active as antibiotics and potential virulence factors. to investigate the role of one of the transcriptional activators of flagellar genes, flhdc, in motility and virulen ... | 2000 | 10613869 |
| photorhabdus toxins: novel biological insecticides. | following concerns over the potential for insect resistance to insecticidal bacillus thuringiensis toxins expressed in transgenic plants, there has been recent interest in novel biological insecticides. over the past year there has been considerable progress in the cloning of several alternative toxin genes from the bacteria photorhabdus luminescens and xenorhabdus nematophilus. these genes encode large insecticidal toxin complexes with little homology to other known toxins. | 1999 | 10383860 |
| isolation and entomotoxic properties of the xenorhabdus nematophilus f1 lecithinase. | xenorhabdus spp. and photorhabdus spp., entomopathogenic bacteria symbiotically associated with nematodes of the families steinernematidae and heterorhabditidae, respectively, were shown to produce different lipases when they were grown on suitable nutrient agar. substrate specificity studies showed that photorhabdus spp. exhibited a broad lipase activity, while most of the xenorhabdus spp. secreted a specific lecithinase. xenorhabdus spp. occur spontaneously in two variants, phase i and phase i ... | 1998 | 9647801 |
| nematophin, a novel antimicrobial substance produced by xenorhabdus nematophilus (enterobactereaceae). | a new antibiotic, nematophin, was isolated from strain bc1 of xenorhabdus nematophilus and detected in all strains of x. nematophilus studied. its structure is fully established as 3-indoleethyl (3'-methyl-2'-oxo)pentanamide by extensive spectroscopic study. the production of nematophin is affected by the strain type and culture conditions. the compound shows strong in vitro bioactivity against a series of fungal and bacterial species. | 1997 | 9304787 |
| a homoserine lactone autoinducer regulates virulence of an insect-pathogenic bacterium, xenorhabdus nematophilus (enterobacteriaceae). | n-beta-hydroxybutanoyl homoserine lactone (hbhl), the autoinducer of the luminescent system of vibrio harveyi, has been identified as the first small compound to restore virulence to avirulent mutants of xenorhabdus nematophilus. hbhl stimulated the level of lipase activity excreted by avirulent x. nematophilus and lowered the phenoloxidase activity in the hemolymph of insects infected with x. nematophilus, parameters that are both associated with insect pathogenesis. moreover, mortality of the ... | 1997 | 9286978 |
| role of the histidine kinase, envz, in the production of outer membrane proteins in the symbiotic-pathogenic bacterium xenorhabdus nematophilus. | we show that inactivation of envz, the gene encoding the histidine kinase sensor protein, envz, of xenorhabdus nematophilus, affected the production of several outer membrane proteins (opns). x. nematophilus produced five major opns during exponential growth. insertional inactivation of envz led to a decrease in the production of opnp, the ompf-like pore-forming protein which constitutes approximately 50% of the total outer membrane protein in x. nematophilus. opna production was also reduced, w ... | 1997 | 9055414 |
| cloning and nucleotide sequence of a flagellin encoding genetic locus from xenorhabdus nematophilus: phase variation leads to differential transcription of two flagellar genes (flicd). | the insect-pathogenic bacterium xenorhabdus undergoes spontaneous phase variation involving a large number of phenotypes. our previous study indicated that phase i variants were motile, whereas phase ii variants of x. nematophilus f1 were nonflagellated cells which did not synthesize flagellin [givaudan a., baghdiguian, s., lanois, a. and boemare, n. (1995) appl. environ. microbiol. 61, 1408-1413]. in order to approach the study of the flagellar switching, a locus containing two orfs from x. nem ... | 1996 | 8996114 |
| phylogenetic relationships of entomopathogenic nematophilic bacteria: xenorhabdus spp. and photorhabdus sp. | phylogenetic relationships of xenorhabdus spp. and photorhabdus sp. were investigated on the basis of 16s rrna gene sequences. xenorhabdus spp. and photorhabdus sp. were grouped together with proteus vulgaris and arsenophonus nasoniae. this group was distant from other members of the family enterobacteriaceae. xenorhabdus japonicus, previously proposed as a new species, was nearly located to xenorhabdus nematophilus. signature nucleotides of x. japonicus were identified that distinguish it other ... | 1996 | 8914266 |
| chitinase activity of xenorhabdus and photorhabdus species, bacterial associates of entomopathogenic nematodes. | xenorhabdus nematophilus (three strains), xenorhabdus bovienii (one strain), and photorhabdus luminescens (one strain) showed both exo- and endochitinase activity using p-nitrophenyl-n-acetyl-beta-d-glucosaminide and p-nitrophenyl-beta-d-n,n',n'-triacetylchitotriose, respectively, as substrates. one to three bands were detected on page gel with glycol chitin after electrophoresis. variation in exo- and endochitinase activity among different species and strains was detected with the strongest act ... | 1996 | 8858906 |
| functional and regulatory analysis of the ompf-like porin, opnp, of the symbiotic bacterium xenorhabdus nematophilus. | the function and novel regulation of opnp of the symbiotic/pathogenic bacterium, xenorhabdus nematophilus was studied. in vitro pore-function analysis of purified opnp indicated that the single-channel-conductance values were similar to that measured for the porin protein, ompf, of esherichia coli. nucleotide sequence analysis revealed that the mature opnp protein contained 348 amino acid residues and shared 55% amino acid sequence identity with ompf. similar to ompf, opnp mapped between asns an ... | 1995 | 8817498 |
| molecular analysis of the two-component genes, ompr and envz, in the symbiotic bacterium xenorhabdus nematophilus. | in escherichia coli the histidine kinase sensor protein, envz, undergoes autophosphorylation and subsequently phosphorylates the regulatory protein, ompr. modulation of the levels of ompr-phosphate controls the differential expression of ompf and ompc. while the phosphotransfer reaction between envz and ompr has been extensively studied, the domains involved in the sensing function of envz are not well understood. we have used a comparative approach to study the sensing function of envz. during ... | 1995 | 8801419 |
| interaction of mutants of xenorhabdus nematophilus (enterobacteriaceae) with antibacterial systems of galleria mellonella larvae (insecta: pyralidae). | xenorhabdus nematophilus mutants that took longer to kill insects than did the wild type were used to determine the relationship of the physicochemical properties and outer membrane composition to bacterial interaction with the antibacterial systems of galleria mellonella larvae and to bacterial virulence. insect serum slowed the growth of the wild-type and mutant bacteria. this was attributed to increased spheroplast formation for the mutants. spheroplast formation was associated with an increa ... | 1994 | 8012904 |
| characterization and environmental regulation of outer membrane proteins in xenorhabdus nematophilus. | we have examined the production of the outer membrane proteins of the primary and secondary forms of xenorhabdus nematophilus during exponential- and stationary-phase growth at different temperatures. the most highly expressed outer membrane protein of x. nematophilus was opnp. the amino acid composition of opnp was very similar to those of the porin proteins ompf and ompc of escherichia coli. n-terminal amino acid sequence analysis revealed that residues 1 to 27 of the mature opnp shared 70 and ... | 1995 | 7887602 |
| purification and characterization of xenorhabdicin, a phage tail-like bacteriocin, from the lysogenic strain f1 of xenorhabdus nematophilus. | xenorhabdicin, the phage tail-like bacteriocins of xenorhabdus nematophilus, and phage head particles, elements produced together after mitomycin induction in x. nematophilus lysogenic strain f1 cultures, were separated by deae chromatography, examined by transmission electron microscopy, and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. electrophoresis of xenorhabdicin showed two major subunits of 43 and 20 kda corresponding to the sheath and the inner core, respec ... | 1995 | 7646048 |
| antimicrobial activity and biosynthesis of indole antibiotics produced by xenorhabdus nematophilus. | we have investigated the mechanism of action and physiology of production of the indole derivative antibiotics produced by the nematode-associated, entomopathogenic bacterium xenorhabdus nematophilus. maximum antibiotic concentration was reached during the late stationary phase of growth, and the antibiotic yield was appreciably enhanced by supplementation with tryptophan. antibiotic biosynthesis apparently involved the removal of the side-chain carboxyl (c-1) carbon of tryptophan. the c-3 methy ... | 1993 | 7510325 |
| relationship of rumen volatile fatty acids to type of grain,sorghum grain processing method and feedlot performance. | 1972 | 5055758 | |
| ultrastructure of achromobacter nematophilus poinar et thomas bacteria. | 1972 | 5052048 | |
| xanthine overdose. | 2016 | 5029168 | |
| [effects of repeated interventions in ear surgery]. | 2016 | 5020044 | |
| preparation of agar wells for antibiotic assay. | 2016 | 4971723 | |
| significance of achromobacter nematophilus poinar and thomas (achromobacteraceae: eubacteriales) in the development of the nematode, dd-136 (neoaplectana sp. steinernematidae). | 1966 | 4960247 | |
| sensitivity of klebsiella, enterobacter, and escherichia coli to cephalothin. | 1969 | 4885058 | |
| [clinical evaluation of gentamicin garamycin in the treatment of postpartum and post-abortion infectious complications]. | 2016 | 4779222 | |
| the use of his-bundle electrocardiography in conduction disturbances of the heart. | 2016 | 4758244 | |
| [preventive vaccinations in allergic patients. 2]. | 2016 | 4755656 | |
| [determining the borderline between stage i-a and stage i-b of carcinoma of the uterine cervix]. | 2016 | 4742615 | |
| the nucleolar organizer of plethodon cinereus cinereus (green). i. location of the nucleolar organizer by in situ nucleic acid hybridization. | 2016 | 4730559 | |
| pseudomyopia as a presenting sign in ocular myasthenia gravis. | 2016 | 4706420 | |
| [papilledema in spinal neoplasms]. | 2015 | 4638163 | |
| the expectations and realities of kidney transplant patients. | 2015 | 4615251 | |
| a universal device for positive end-expiratory pressure (peep). | 2015 | 4585936 | |
| biogenesis of mitochondria. xxv. studies on the mitochondrial genomes of petite mutants of yeast using ethidium bromide as a probe. | 1973 | 4583801 | |
| the mechanism of action of beta-galactosidase. effect of aglycone nature and -deuterium substitution on the hydrolysis of aryl galactosides. | 1. steady-state kinetic parameters for the beta-galactosidase-catalysed hydrolysis of 13 aryl beta-d-galactopyranosides show no simple dependence on aglycone acidity. 2. alpha-deuterium kinetic isotope effects (k(h)/k(d)) for seven of these substrates, measured under steady-state conditions with [s]>>k(m), vary from 1.00 for poor substrates to 1.25 for hydrolysis of the galactosyl-enzyme. 3. methanolysis of the galactosyl-enzyme in 1.5m-methanol increases k(h)/k(d) for degalactosylation, but lea ... | 1973 | 4578762 |
| [history of cataract treatment]. | 2015 | 4575472 | |
| bilateral tubal pregnancy with an unusual complication. | 1973 | 4570658 | |
| phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. | 1972 | 4562620 | |
| current reference works in public health. | 2015 | 4557014 | |
| [functional disorders of the biliary tracts]. | 2015 | 4553777 | |
| biochemical aspects of some pig muscle disorders. | 1972 | 4553607 | |
| [response of the recipient's tissue bed and features of reparative osteogenesis in retransplantation of bone tissue]. | 1972 | 4553209 | |
| [60th birthday of prof. václav trnka, dr. sc]. | 2015 | 4551569 | |
| antibody-dependent lymphocyte-mediated cytotoxicity in man: no requirement for lymphocytes with membrane-bound immunoglobulin. | 1973 | 4542262 | |
| cyclizing compounds. 3. local anesthetic action of n-(omega-haloalkyl)-n-methylaminoaceto-2,6-xylidides. | 1973 | 4542166 | |
| direct coronary revascularization. | 2015 | 4542165 | |
| "uterine" and "luteal" effects of prostaglandins (pg) in rats and guinea pigs as potential abortifacient mechanisms. | 1973 | 4542124 | |
| a new dental superalloy system. 3. microstructure and phase transformations. | 2015 | 4509488 | |
| nursing care study: secondary dementia. | 2015 | 4486325 | |
| [specificity of antibodies in experimental trichinosis of the rat]. | 1974 | 4455270 | |
| pletysmographic and intramedullary pressure measurements before and after tibial osteotomy for osteoarthritis of the knee. | 2015 | 4432738 | |
| effect of prostaglandin f2alpha on the luteal phase of the cycle in nonpregnant women. | 11 healthy nonpregnant female volunteers were treated with (pgf) prostaglandin f2alpha as the tham salt in aqueous solution administratered intravaginally. (hcg) human chorionic gonadotropins was also administered to 9 of these women prior to or following the pg therapy to simulate secreting the phoblast. it was considered that luteolysis occurred if serum progestin declined to preovulatory levels, if there was no increase of serum progestin levels following hcg, and if there was shortening of ... | 1974 | 4429112 |
| [skeletal traction in the treatment of pelvic bone fractures]. | 2015 | 4421797 | |
| on the possible origin and evolution of the genetic code. | 2015 | 4414917 | |
| a hydrogen-bond network at the active site of subtilisin bpn'. | 1970 | 4399039 | |
| effects of steroid and nonsteroid metabolites on enzyme conformation and pyridoxal phosphate binding. | 1969 | 4391266 | |
| [muscarinic receptors of the superior cervical lymph node of the cat]. | 1966 | 4386667 | |
| suppression of herpes simplex virus infection by phosphonoacetic acid. | disodium phosphonoacetate when administered orally or topically to mice experimentally infected with herpes simplex virus was able to significantly reduce the mortality associated with the agent. in addition, this compound was able to reduce herpesvirus lesions on the corneas of infected rabbits. | 1973 | 4356456 |
| investigation on the epidemiological significance of detailed cancer data--preliminary results. | 2015 | 4352667 | |
| effects of photooxidation of histidine-38 on the acetylphosphatase activity of glyceraldehyde-3-phosphate dehydrogenase. | 1973 | 4345586 | |
| [electrophysiologic, histochemical and immunologic correlates in the central mechanism of audiogenic epilepsy]. | 1972 | 4336346 | |
| a myoglobin-type haemoglobin of chironomus thummi thummi. | 2015 | 4333654 | |
| isolation of heart-resistant clostridium perfringens from healthy cattle. | 1972 | 4333445 | |
| [ilioinguinal syndrome]. | 2015 | 4332489 | |
| [stabilization of n-antigens of enteroviruses]. | 1970 | 4322838 | |
| [the process of maturation of the proximal epiphyses of both bones of the forearm in the female]. | 2014 | 4288884 | |
| [the process of maturation of the proximal epiphyses of both bones of the forearm in the female]. | 2014 | 4288884 | |
| free and conjugated estrogens of an ovarian cystadenoma and granulosa cell tumor. | 2014 | 4284085 | |
| muscle membrane protein kinase in myotonic muscular dystrophy. | 2014 | 4277586 | |
| muscle membrane protein kinase in myotonic muscular dystrophy. | 2014 | 4277586 | |
| studies on the apparent instability of neurospora tryptophan synthase. evidence for protease. | 2014 | 4265723 | |
| chemotherapy of cancer of the colon and rectum. | 2014 | 4260654 | |
| [arthrocele in rheumatic knee joint diseases]. | 2014 | 4234257 | |
| [treatment of eyelid epitheliomas by betatron]. | 2014 | 4219756 | |
| the vaccination of man and other animals against rabies. | 1973 | 4207636 | |
| fluorouracil and radiotherapy in gastrointesinal cancer. | 2014 | 4187834 | |
| hidden antigenic determinant in secretory immunoglobulin a. | 1968 | 4176458 | |
| [detection and identification of bezo-(f)-diazepine-1,4 derivatives in urine]. | 2014 | 4156460 | |
| analgesia produced by clonidine in mice and rats. | 1974 | 4152777 | |
| studies on the therapeutic use of mercurascan (msc). part 2: antiarrhythmogenic and haemodynamic effect of msc in experimental myocardial ischaemia. | 1974 | 4140846 | |
| [cytodiagnosis of tumors of the gastrointestinal system]. | 2014 | 4135741 | |
| tentative immunochemical analysis of the prothrombin molecule. | 2014 | 4122342 | |
| improvement in mental depression with decreased thyrotropin response after administration of thyrotropin-releasing hormone. | 2014 | 4116150 | |
| [development of polyribosomes during maturation of reticulocytes in vivo]. | 1970 | 4098024 | |
| [immunological study of anti-cancer effects of human alpha interferon for advanced renal cell carcinoma]. | 1985 | 4079199 | |
| posttraumatic acalculous cholecystitis. | 2014 | 4068695 | |
| physiological stress and performance changes in response to beginning level orienteering. | 2014 | 4068683 | |
| transdermally administered scopolamine. | 2014 | 4063911 | |
| diurnal rhythms in calcium and phosphate metabolism in rodents and their relations to lighting and feeding schedules. | diurnal fluctuations in serum ca, inorganic phosphorus, mg, and alkaline phosphatase activity and their relations to lighting and feeding schedules were investigated in nocturnal rats and diurnal asiatic chipmunks. the following results were obtained: all parameters in the animals fed ad libitum and adapted to a regulated light-dark cycle (with light from 7.00 to 19.00 h) showed diurnal rhythms; under the same experimental condition, the patterns of the rhythms in the nocturnal rat and the diurn ... | 1985 | 4040208 |
| [hyperbaric oxygenation in combined therapy of viral hepatitis]. | 2012 | 4036041 | |
| [bilateral wrist fractures]. | 2014 | 4017731 | |
| electrophysiological studies of myoclonic jerks provoked by photic stimulation. | a patient with a type of myoclonus produced by somatosensory and photic stimuli was studied electrophysiologically. jerk-locked averaging showed cortical spikes in association with the spontaneous myoclonus. high amplitude somatosensory evoked potentials and high amplitude visual evoked potentials were observed. somatosensory and photic stimuli produced 2 or 3 successive electromyographic (emg) discharges. the time interval from the onset of the preceding emg discharge to that of the following e ... | 2014 | 4013664 |
| [studies on male sexual impotence. report xvii: an attempt to evaluate therapeutic effect on psychogenic impotence by a logarithmic scoring system]. | 2009 | 4010113 |