Publications

Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae.	In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia colialdH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield re ...	2012	22055092
[still increasing incidence of mrsa and esbl-producing enterobacteriaceae in society and in hospital-associated cases].	this review describes the recent epidemiology for mrsa and esbl-producing enterobacteriaceae in denmark. mrsa community-associated cases continue to increase whereas hospital associated cases are low and stable. due to an active search and destroy policy secondary transmission is modest. mrsa from pigs is an increasing problem. for esbl producing escherichia coli a considerable multi clonal increase has been seen both in the community and in hospitals. there are indications on food being a signi ...	2011	22053803
reduction of molybdate to molybdenum blue by klebsiella sp. strain hkeem.	a novel molybdate-reducing bacterium, tentatively identified as klebsiella sp. strain hkeem and based on partial 16s rdna gene sequencing and phylogenetic analysis, has been isolated. strain hkeem produced 3 times more molybdenum blue than serratia sp. strain dr.y8; the most potent mo-reducing bacterium isolated to date. molybdate was optimally reduced to molybdenum blue using 4.5 mm phosphate, 80 mm molybdate and using 1% (w/v) fructose as a carbon source. molybdate reduction was optimum at 30 ...	2011	22052341
[pneumonia-causing bacterial pathogens in intensive care patients].	this prospective study aimed at determining etiologic agents causing nosocomial pneumonia in a precisely defined group of patients and resistance of bacterial pathogens to antimicrobial drugs.	2011	22052100
dna, protein binding, cytotoxicity, cellular uptake and antibacterial activities of new palladium(ii) complexes of thiosemicarbazone ligands: effects of substitution on biological activity.	the coordination propensities of 4(n,n')-diethylaminosalicylaldehyde-4(n)-substituted thiosemicarbazones (h(2)l(1-4)) were investigated by reacting with an equimolar amount of [pdcl(2)(pph(3))(2)]. the new complexes were characterized by various spectroscopic techniques. the structure determination of the complexes [pd(deasal-tsc)(pph(3))] (1), [pd(deasal-mtsc)(pph(3))] (2) and [pd(deasal-etsc)(pph(3))] (3) by x-ray crystallography showed that ligands are coordinated in a dibasic tridentate ons ...	2011	22051854
synthesis and kinetic testing of new inhibitors for a metallo-β-lactamase from klebsiella pneumonia and pseudomonas aeruginosa.	there are currently no clinically useful inhibitors against metallo-β-lactamases (mbls), enzymes that confer resistance against a broad spectrum of commonly used antibiotics and that are produced by an increasing number of bacterial pathogens. new pyrrole derivatives were synthesized and assayed for their inhibitory effect on the catalytic activity of the imp-1 mbl from pseudomonas aeruginosa and klebsiella pneumoniae. six compounds tested (3a-3c, 5, 7 and 8) show micromolar inhibition constants ...	2011	22051063
in-vitro activity of three brands of ceftriaxone against different clinical isolates.	ceftriaxone is an extended spectrum cephalosporin that has been used for decades for combating infections caused by wide array of pathogens and prescription of antibiotic is in favor of specific brands without realizing the potency of the brand. the in-vitro activity of three brands of ceftriaxone and various antibiotics against clinical isolates is studied.	2010	22049828
characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants.	this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ...	2012	22047662
[Aspects of the antimicrobial resistence profile in infections with Escherichia coli and Klebsiella pneumoniae in diabetic patients].	Infections in diabetic patient remains an important cause of morbidity and mortality, triggering and maintaining a prolonged metabolic imbalance. Emergence of extented spectrum beta-lactmase (ESBL) in Escherichia coli and Klebsiella pneumoniae is a major concern, because of the atypical manner infection acts in this group of imunodepressed patients and also for the limited therapeutic solutions. For this reason we have evaluated the profile of antimicrobial resistance of these pathogens in both ...	2011	22046785
molecular dissection of an outbreak of carbapenem-resistant enterobacteriaceae reveals intergenus kpc carbapenemase transmission through a promiscuous plasmid.	carbapenem-resistant enterobacteriaceae (cre) have emerged as major causes of health care-associated infections worldwide. this diverse collection of organisms with various resistance mechanisms is associated with increased lengths of hospitalization, costs of care, morbidity, and mortality. the global spread of cre has largely been attributed to dissemination of a dominant strain of klebsiella pneumoniae producing a serine β-lactamase, termed k. pneumoniae carbapenemase (kpc). here we report an ...	2011	22045989
resistance pattern of ciprofloxacin against different pathogens.	ciprofloxacin is a broad-spectrum antibiotic widely prescribed in clinical and hospital settings. the emergence of antimicrobial resistance against effective antibiotics is a global issue. the objective of study is the surveillance of ciprofloxacin against common pathogens.	2010	22043361
prevalence of uropathogens in diabetic patients and their corresponding resistance pattern: results of a survey conducted at diagnostic centers in dhaka, bangladesh.	urinary tract infection is the second most common clinical indication for empirical antibiotic treatment in primary and secondary health care settings. the incidence of diabetes mellitus throughout the world is increasing strikingly and in the long run, it has some major effects on the genitourinary system which makes diabetic patients more liable to urinary tract infection. this study is designed to reveal the distribution of uropathogens in diabetic patients according to age and sex, and corre ...	2010	22043358
in vitro susceptibility test of different clinical isolates against ceftriaxone.	because of the prevailing penicillin resistance in microorganisms, broad spectrum cephalosporins are used empirically specially in developing countries. the aim of this study is to determine the susceptibility pattern of different gram positive and gram negative pathogens against third generation cephalosporin-ceftriaxone to explore the existing effectiveness of this antibiotic.	2010	22043337
evolution to carbapenem-hydrolyzing activity in noncarbapenemase class d β-lactamase oxa-10 by rational protein design.	class d β-lactamases with carbapenemase activity are emerging as carbapenem-resistance determinants in gram-negative bacterial pathogens, mostly acinetobacter baumannii and klebsiella pneumoniae. carbapenemase activity is an unusual feature among class d β-lactamases, and the structural elements responsible for this activity remain unclear. based on structural and molecular dynamics data, we previously hypothesized a potential role of the residues located in the short-loop connecting strands β5 ...	2011	22042844
Enhancement of pH stability and activity of glycerol dehydratase from Klebsiella pneumoniae by rational design.	Glycerol dehydratase (GDHt) is a key and rate-limiting enzyme in the pathway of 1,3-propanediol (1,3-PD) synthesis. The improvement of GDHt's stability and enzymatic activity is desirable for the biosynthesis of 1,3-PD. The gldABC gene encoding GDHt of Klebsiella pneumoniae was cloned and expressed in Escherichia coli XL10-Gold, and the mutation sites of GDHt were obtained through prediction by PoPMuSiC program. Consequently, two mutants (KpG60 and KpG525) were developed by rational design throu ...	2011	22042303
screening method for detection of immediate amino acid decarboxylases--producing bacteria implicated in food poisoning.	assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. in this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. møller decarboxylase base broth (mdb) with or without 1% of a known amino acid were added to wells of a 96 well-microtitre plate. the wells were inoculated with escherichia coli, klebsiella pneumoniae, acinetobacter anitr ...	2011	22041756
In vitro activity of avibactam (NXL104) in combination with ß-lactams against Gram-negative bacteria, including OXA-48 ß-lactamase-producing Klebsiella pneumoniae.	The objective of this study was to investigate the in vitro antibacterial activity of avibactam (formerly NXL104) in combination with imipenem, cefepime or ceftazidime against Gram-negative bacteria. Bacterial isolates included: Pseudomonas aeruginosa harbouring PER-1 ß-lactamase (n=14); Acinetobacter baumannii harbouring PER-1, OXA-51 and OXA-58 (n=20); carbapenem-non-susceptible Klebsiella pneumoniae (n=25) and Escherichia coli (n=1) harbouring OXA-48; carbapenem-non-susceptible E. coli (n=1) ...	2012	22041508
clinical characteristics of urosepsis caused by extended-spectrum beta-lactamase-producing escherichia coli or klebsiella pneumonia and their emergence in the community.	background: the purpose of this study is to delineate clinical characteristics of urosepsis caused by extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae (esbl-ek) in different clinical settings, with an emphasis on community-acquired infections. methods: a retrospective study was conducted at two medical centers in taiwan. from may 2002 to august 2007, clinical data of adults with urosepsis caused by esbl-ek were collected. patients were categorized into three ...	2011	22041167
response changes as gram neg hais rise.		2011	22039619
hypervirulent k. pneumoniae secretes more and more active iron-acquisition molecules than "classical" k. pneumoniae thereby enhancing its virulence.	a new hypervirulent (hypermucoviscous) clinical variant of klebsiella pneumoniae (hvkp) has emerged over the last decade. our goal is to identify new mechanisms, which increase the virulence hvkp compared to "classic" k. pneumoniae (ckp).	2011	22039542
hepg2 cells infected with klebsiella pneumoniae show dna laddering at apoptotic and necrotic stages.	virulent klebasiella pneumoniae (kp) inflicts severe liver abscesses in infected patients. this study investigated how the bacterial infection affected cell survival at the molecular level, in a cultured cell model. a strain of kp highly virulent in mice was isolated from a patient with liver abscess, and was used to infect hepg2 cells. the infected cells were examined for their viability, dna fragmentation, and proteins involved in apoptosis or necrosis. we found that the infection decreased th ...	2011	22038342
Thermal treatment of the bacteriophage lysate of Klebsiella pneumoniae B5055 as a step for the purification of capsular depolymerase enzyme.	The lytic bacteriophage which produces the hydrolase enzyme capable of depolymerizing exopolysaccharide of Klebsiella pneumoniae and of other bacteria was isolated earlier. In this study, a simple method of depolymerase purification from the phage lysate by dissociating the enzyme from the phage particle was developed. The bacteriophage showed a relatively smaller plaque size surrounded by a wide halo indicating a depolymerase action on the capsular polysaccharide of K. pneumoniae B5055. The dep ...	2012	22036659
clinical characteristics and outcomes of patients with extended-spectrum β-lactamase-producing bacteremias in the emergency department.	extended-spectrum β-lactamase (esbl)-producing bacteria have been spreading from hospitals to communities. despite this, there are limited emergency department (ed) patient-based studies about these bacteremias. a retrospective matched case-control study with a ratio of 1:3 was conducted at a university hospital. the case group consisted of patients aged >16 years with esbl-producing bacteremias in the ed. patients matched for age and sex with non-esbl-producing bacteremias were sampled as the c ...	2011	22033790
Occurrence of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae isolates.	Adequate detection of the production of carbapenemase in Enterobacteriaceae isolates is crucial for infection control measures and the appropriate choice of antimicrobial therapy. In this study, we investigated the frequency of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae clinical isolates by the modified Hodge test (MHT). Three hundred and one E. coli and K. pneumoniae clinical isolates were investigated. All pro ...	2011	22031829
influence of consumption of probiotics on presence of enterobacteria in the oral cavity.	clinical benefits of probiotics have been clearly reported in different gastrointestinal disorders, many of them caused by enterobacteria. the oral cavity is a port of entry and can be an important reservoir of these microorganisms. this work evaluated whether consumption of probiotics was able to influence the presence of enterobacteria in the oral cavity and the specific secretory response against these microorganisms. saliva samples of healthy individuals were collected and plated in macconke ...	2011	22031052
[antimicrobial resistance pattern for gram-negative uropathogens isolated from hospitalised patients and outpatients in cartagena, 2005-2008].	determining the microbial aetiology spectrum and antibiotic resistance pattern of uropathogens causing urinary tract infections in hospitalised patients and outpatients.	2010	22030688
clinical predictors of pseudomonas aeruginosa or acinetobacter baumannii bacteremia in patients admitted to the ed.	the identification of clinical characteristics that could identify patients at high risk for pseudomonas aeruginosa or acinetobacter baumannii bacteremia would aid clinicians in the appropriate management of these life-threatening conditions, especially in patients admitted to the emergency department (ed) with community-onset infections. to determine clinical risk factors for p aeruginosa or a baumannii bacteremia in patients with community-onset gram-negative bacteremia (gnb), a post hoc analy ...	2011	22030178
aberrant structures of fecal bacterial community in allergic infants profiled by 16s rrna gene pyrosequencing.	we investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. the fecal microbiota in the first 2 months was profiled using the 16s rrna v6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). in the allergic group, genus bacteroides at 1 month and genera propionibacterium and klebsiella at 2 mont ...	2011	22029688
characterization of purified new delhi metallo-β-lactamase-1.	new delhi metallo-β-lactmase-1 (ndm-1) has recently emerged as a global threat because of its ability to confer resistance to almost all clinically used β-lactam antibiotics, its presence within an easily transmissible plasmid bearing a number of other antibiotic resistance determinants, its carriage in a variety of enterobacteria, and its presence in both nosocomial and community-acquired infections. to improve our understanding of the molecular basis of this threat, ndm-1 was purified and char ...	2011	22029287
identification of an antimicrobial peptide from human methionine sulfoxide reductase b3.	human methionine sulfoxide reductase b3a (hmsrb3a) is an endoplasmic reticulum (er) reductase that catalyzes the stereospecific reduction of methionine-r-sulfoxide to methionine in proteins. in this work, we identified an antimicrobial peptide from hmsrb3a protein. the n-terminal er-targeting signal peptide (amino acids 1-31) conferred an antimicrobial effect in escherichia coli cells. sequence and structural analyses showed that the overall positively charged er signal peptide had an argand pro ...	2011	22027001
[In vitro evaluation of bactericidal activity of an over-heated saturated dry atomized steam electro-medical device on inert contaminated surfaces].	Surface disinfection together with cleaning practises and aseptic procedures are established measures for effective prevention of infectious diseases reducing infection risks. In this study, we evaluated the bactericidal effectiveness in vitro of the electro-medical device Sani System, an over-heated saturated dry-steam disinfection system, against predetermined bacterial load dried on inert surfaces. In particular we have tested different materials, representative of operating rooms furnishing ...	2011	22026238
pharmacodynamics of β-lactamase inhibition by nxl104 in combination with ceftaroline: examining organisms with multiple types of β-lactamases.	new broad-spectrum β-lactamases such as kpc enzymes and ctx-m-15 enzymes threaten to markedly reduce the utility of our armamentarium of β-lactam agents, even our most potent drugs, such as carbapenems. nxl104 is a broad-spectrum non-β-lactam β-lactamase inhibitor. in this evaluation, we examined organisms carrying defined β-lactamases and identified doses and schedules of nxl104 in combination with the new cephalosporin ceftaroline, which would maintain good bacterial cell kill and suppress res ...	2012	22024819
Molecular Epidemiology of Ciprofloxacin-Resistant Extended-Spectrum ß-Lactamase-Producing Klebsiella pneumoniae in Taiwan.	Fluoroquinolone resistance in extended-spectrum ß-lactamases (ESBL)-producing isolates results in very few antimicrobial treatment options. In Taiwan's Surveillance of Antimicrobial Resistance (TSAR) III program, 124 (52.8%) cases of ESBL-producing Klebsiella pneumoniae (ESBL-KP) were resistant to ciprofloxacin. The prevalence of plasmid-mediated quinolone resistance (PMQR) determinants and chromosomal quinolone resistance-determining regions (QRDR) of gyrA and parC genes among ESBL-KP isolates ...	2011	22022870
target highlights in casp9: experimental target structures for the critical assessment of techniques for protein structure prediction.	one goal of the casp community wide experiment on the critical assessment of techniques for protein structure prediction is to identify the current state of the art in protein structure prediction and modeling. a fundamental principle of casp is blind prediction on a set of relevant protein targets, that is, the participating computational methods are tested on a common set of experimental target proteins, for which the experimental structures are not known at the time of modeling. therefore, th ...	2011	22020785
Exploring the DNA binding mode of transition metal based biologically active compounds.	Few novel 4-aminoantipyrine derived Schiff bases and their metal complexes were synthesized and characterized. Their structural features and other properties were deduced from the elemental analysis, magnetic susceptibility and molar conductivity as well as from mass, IR, UV-vis, (1)H NMR and EPR spectral studies. The binding of the complexes with CT-DNA was analyzed by electronic absorption spectroscopy, viscosity measurement, and cyclic voltammetry. The interaction of the metal complexes with ...	2012	22020164
production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase pdup mediated biosynthetic pathway in klebsiella pneumoniae.	the pdup gene encodes a propionaldehyde dehydrogenase (pdup) was investigated for the role in 3-hydroxypropionic acid (3-hp) glycerol metabolism in klebsiella pneumoniae. the enzyme assay showed that cell extracts from a pdup mutant strain lacked measurable dehydrogenase activity. additionally, the mutant strain accumulated the cytotoxic intermediate metabolite 3-hydroxypropionaldehyde (3-hpa), causing both cell death and a lower final 3-hp titer. ectopic expression of pdup restored normal cell ...	2012	22018749
isolation of a klebsiella pneumoniae isolate of sequence type 258 producing kpc-2 carbapenemase in korea.	carbapenem-resistant klebsiella pneumoniae isolates producing k. pneumoniae carbapenemases (kpc) were first reported in the usa in 2001, and since then, this infection has been reported in europe, israel, south america, and china. in korea, the first kpc-2-producing k. pneumoniae sequence type (st) 11 strain was detected in 2010. we report the case of a patient with a urinary tract infection caused by kpc-2-producing k. pneumoniae. this is the second report of a kpc-2-producing k. pneumoniae inf ...	2011	22016687
prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase and ampc beta-lactamase in enterobacteriaceae.	we investigated the prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase (esbl) and ampc beta-lactamase in enterobacteriaceae.	2011	22016679
klebsiella pneumoniae type 3 fimbriae agglutinate yeast in a mannose resistant manner.	the ability of bacterial pathogens to express different fimbrial adhesins plays a significant role in virulence. thus, specific detection of fimbrial expression is an important task in virulence characterization and epidemiological studies. most clinical klebsiella pneumoniae isolates express type 1 and type 3 fimbriae characterized by mediating mannose sensitive agglutination of yeast cells, and agglutination of tannic acid treated ox red blood cells (rbcs), respectively. we have observed that ...	2011	22016558
Isolation and identification of antibacterial compound from the leaves of Cassia auriculata.	Antimicrobial properties of medicinal plants and plant parts such as flowers, roots, fruits, seeds and oils are being used to cure some chronic and acute diseases throughout the world. In the present study, an attempt has been made to isolate and identify the antibacterial compound present in the leaves of the Cassia auriculata.	2011	22013726
Carbapenem-resistant Klebsiella pneumoniae associated with a long-term--care facility --- West Virginia, 2009-2011.	On January 27, 2011, a West Virginia county health department was notified of a cluster of carbapenem-resistant Klebsiella pneumoniae (CRKP) cases detected by a local hospital (hospital A). CRKP infections frequently are resistant to a majority of antimicrobial agents and have an increased risk for morbidity and mortality. The West Virginia Bureau for Public Health (WVBPH) conducted field investigations to identify all cases, characterize risk factors for infection, and abstract data for a match ...	2011	22012114
sensitive and specific modified hodge test for kpc and metallo-beta- lactamase detection in pseudomonas aeruginosa by use of a novel indicator strain, klebsiella pneumoniae atcc 700603.	we evaluated the ability of the modified hodge test to discriminate between kpc- and metallo-beta-lactamase (mbl)-producing pseudomonas aeruginosa isolates and carbapenemase nonproducers. with escherichia coli atcc 25922 as the indicator strain, the mht resulted in low sensitivity, specificity, and repeatability. replacing the indicator strain with klebsiella pneumoniae atcc 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeata ...	2011	22012019
[Review of the effectiveness of an empirical antibiotic therapy in suspected ventilator-associated pneumonia].	Ventilator-associated pneumonia (VAP) occurs in approximately 10-20% of mechanically ventilated patients, and is associated with an extremely high mortality rate (up to 70%). The purpose of the study was to determine the susceptibility spectrum of Klebsiella, Pseudomonas and Acinetobacter strains isolated from VAP patients.	2011	22011920
Increasing burden of hospital acquired infections: resistance to cephalosporin antibiotics among klebsiella and Escherichia coli.	The drug susceptibility pattern of klebsiella species and Escherichia coli to cephalosporins was examined in the year 2004 and 2005. A total of 180 klebsiella and 220 E coli in 2004 and 95 klebsiella and 210. E coli in the year 2005, isolated from various clinical specimens were studied. Resistance rates among the klebsiella species, ranged from 50.00%-86.36% and 42.10%-84.21% in 2004 and 2005 respectively. Resistance rates among E coli ranged from 46.66%-90.8% and 50.0%-86.66% in 2004 and 2005 ...	2011	22010583
Necrotic intramuscular chloroma with infection: magnetic resonance imaging features.	We recently experienced the case of an intramuscular chloroma with infection in a 7-year-old boy diagnosed with acute myeloid leukemia. Conventional magnetic resonance imaging (MRI) showed that the lesion mimicked an abscess, but diffusion-weighted imaging showed no diffusion restriction. These results suggested that the interior cystic portion was serous. On histopathological findings, a chloroma was diagnosed on the wall of a mass. Culture of the interior fluid revealed that Klebsiella pneumon ...	2011	22009427
Detection of Citrobacter koseri carrying beta-lactamase KPC-2 in a hospitalised patient, Greece, July 2011.	This report describes the detection of Citrobacter koseri carrying K. pneumoniae carbapenemase (KPC-2) isolated in July 2011 from a Greek patient, who was also colonised by a Klebsiella pneumoniae strain coproducing KPC-2 and Verona integron-encoded metallo-beta-lactamase (VIM)-1.	2011	22008199
[the changing epidemiology of extended spectrum β-lactamase-producing klebsiella pneumoniae].	in the last two decades, klebsiella pneumoniae demonstrated some characteristics of acquisition of plasmids coding extended spectrum β-lactamases (esbl). the review data showed an increase in worldwide prevalence of esbl and a temporal shift in the prevalence of esbl types in k. pneumoniae during this last decade. ctx-m-15 seems to be the predominant esbl type in k. pneumoniae in some parts of the world. the dissemination of several nosocomial ctx-m-15-producing k. pneumoniae clones was reported ...	2011	22008131
chemical composition and antimicrobial activity of the essential oil of anthemis altissima l. var. altissima.	the essential oil obtained from the flowering parts of anthemis altissima l. var. altissima was analysed by gas chromatography and gas chromatography mass spectroscopy. in this study, 34 compounds representing 98.76% of the essential oil were identified. the main components were α-terpineol (26.42%), β-pinene (9.23%), cis-chrysanthenyl acetate (6.30%), globulol (5.36%), n-tricosane (4.41%), terpinen-4-ol (4.08%) and 1,8 cineole (3.84%). antibacterial activities of the essential oil and its two m ...	2011	22007714
defense role of the cocoon in the silk worm bombyx mori l.	silk from the domesticated silk worm bombyx mori procures foreign body response naturally, so it has been utilized as a biomaterial for decades. in india the prime focus of the sericulture industry is to improve silk production with high quality silk. naturally, the silk worm builds its cocoon not only with silk proteins, but also with antimicrobial proteins to avoid infection since the cocoon is non-motile and non-feeding. the aim of the present study is to elucidate the antimicrobial proteins ...	2011	22006381
ndm-1-producing klebsiella pneumoniae in mauritius.		2012	22006002
Nosocomial Outbreak of VIM-1-Producing Klebsiella pneumoniae Isolates of Multilocus Sequence Type 15: Molecular Basis, Clinical Risk Factors, and Outcome.	We study the epidemiology, molecular basis, clinical risk factors, and outcome involved in the clonal dissemination of VIM-1-producing Klebsiella pneumoniae isolates in the hospital setting. All patients infected/colonized by carbapenem-nonsusceptible K. pneumoniae (CNSKP) in 2009 were included. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antibiotic resistance genes were analyzed by PCR and sequencing. Plasmids were studied ...	2012	22005997
green fluorescent protein (gfp)-labeling of enterobacteria associated with fruit flies (diptera: tephritidae) and persistence in their natural host rhagoletis completa cresson.	fruit flies (diptera: tephritidae) are a highly successful, widespread group of insects that cause economic damage in agriculture. data available so far on the composition of the bacterial community associated with their digestive tract indicate that members of enterobacteriaceae are the species most often isolated. bacteria naturally occurring in insect guts may be engineered and used to study the spatial and functional interactions of microbes within the insect system and offer one route to me ...	2011	22004066
a case of gas-forming liver abscess with diabetes mellitus.	a 43-year-old man was admitted to our hospital with right hypochondriac and epigastric pain. an abdominal radiograph showed a large niveau in the right subphrenic space. an abdominal ct scan demonstrated a large liver abscess (diameter, 13 cm) with gas formation. klebsiella pneumoniae cells were isolated from the abscess, and the patient was treated with antibiotics and percutaneous drainage. it is very important to treat gas-forming liver abscess immediately, because subsequent bacteremia and s ...	2011	22001459
antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpod gene.	gram-negative bacteria (gnb) cause common and severe hospital- and community-acquired infections with a high incidence of multidrug resistance (mdr) and mortality. the emergence and spread of mdr-gnb strains limit therapeutic options and highlight the need to develop new therapeutic strategies. in this study, the peptide (rxr)(4)xb- and (kff)(3)k-conjugated peptide nucleic acids (ppnas) were developed to target rpod, which encodes an rna polymerase primary σ(70) that is thought to be essential f ...	2011	22000398
global spread of carbapenemase-producing enterobacteriaceae.	carbapenemases increasingly have been reported in enterobacteriaceae in the past 10 years. klebsiella pneumoniae carbapenemases have been reported in the united states and then worldwide, with a marked endemicity at least in the united states and greece. metallo-enzymes (verona integron-encoded metallo-β-lactamase, imp) also have been reported worldwide, with a higher prevalence in southern europe and asia. carbapenemases of the oxacillinase-48 type have been identified mostly in mediterranean a ...	2011	22000347
recurring klebsiella pneumoniae pyogenic liver abscesses in a resident of san diego, california, due to a k1 strain carrying the virulence plasmid.	we report a diabetic patient who had three episodes of cryptogenic liver abscess due to klebsiella pneumoniae. he was a caucasian who lived in california and had no epidemiological connection to east asia. the isolate from his third episode was a hyperviscous k1 strain that carried the klebsiella virulence plasmid.	2011	21998428
imp-producing carbapenem-resistant klebsiella pneumoniae in the united states.	the emergence and spread of carbapenem-resistant enterobacteriaceae (cre) producing acquired carbapenemases have created a global public health crisis. in the united states, cre producing the klebsiella pneumoniae carbapenemase (kpc) are increasingly common and are endemic in some regions. metallo-β-lactamase (mbl)-producing cre have recently been reported in the united states among patients who received medical care in countries where such organisms are common. here, we describe three carbapene ...	2011	21998425
a threat of the klebsiella pneumoniae carbapenemase-producing strains among transplant recipients.	infections due to klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae are associated with increased therapeutic failure and mortality. our laboratory recognized several strains producing kpc, most of which originated from transplantation ward patients.	2011	21996247
strains of klebsiella pneumoniae producing extended spectrum beta-lactamases, isolated from organ recipients.	the aims of this work were to define the effectiveness of identification of the extended-spectrum beta-lactamases (esbl) phenotype, and to define the genotype of klebsiella pneumoniae β-lactamase.	2011	21996244
Comparison of bacteria isolated from urinary tract infections in patients on transplant versus urologic wards.	Urinary tract infections (UTI) are the most common hospital infections. Complications include sepsis and shock. Immunosuppressed transplant surgery patients may experience loss of the graft due to UTI. The purpose of this study was to determine the main microorganisms responsible for UTIs among patients of transplant wards compared to urologic wards. Additionally, drug susceptibility profiles of the most frequent microorganisms were analyzed.	2011	21996243
early mortality and retinopathy of prematurity: report of 2 cases.		2011	21995996
turnover and accessibility of a reentrant loop of the na+-glutamate transporter glts are modulated by the central cytoplasmic loop.	glts of escherichia coli is a secondary transporter that catalyzes na+-glutamate symport. the structural model of glts shows two homologous domains with inverted membrane topology that are connected by a central loop that resides in the cytoplasm. each domain contains a reentrant loop structure. accessibility of the cys residues in two glts mutants in which pro351 and asn356 in the reentrant loop in the c-terminal domain were replaced by cys is demonstrated to be sensitive to the catalytic state ...	2011	21995702
klebsiella spp as a 1, 3-propanediol producer - the metabolic engineering approach.	klebsiella spp are one of the best natural producers of 1,3-propanediol (1,3-pd). however, their usage in the biotechnological production of the diol is limited, since the species belong to the second hazard group. nevertheless, multiple advantageous traits of klebsiella spp justify the international effort devoted to develop a biotechnological process of 1,3-pd production with these microorganisms. apart from the process engineering approach aiming at improvement of 1,3-pd production by klebsie ...	2011	21995522
Extended spectrum â-lactamase producing multidrug resistant clinical bacterial isolates at National Public Health Laboratory, Nepal.	Extended Spectrum â-lactamase (ESBL) producing multidrug resistant bacteria complicate therapeutic management and limit treatment options. Therefore, detection of ESBL-producing multidrug resistant (MDR) pathogens has a paramount importance. Between April 2009 and January 2010, a prospective study was carried out in National Public Health Laboratory with an objective to determine the status of ESBL producing MDR bacterial isolates from different clinical samples. Identification of the isolates w ...	2011	21991699
clinical and bacteriological profile of urinary tract infection in children at nepal medical college teaching hospital.	the aim of this study was to study the clinical and bacteriological profile of urinary tract infection (uti) in children. this was a prospective study of 40 culture positive cases of uti in children who attended pediatric outpatient department of nepal medical college teaching hospital. uti was more common in female (65.0%) than in male (35.0%). half were in the age group 1- 5 years. fever was the most common presentation (65.0%) followed by abdominal pain (42.5%), decreased appetite (37.5%) and ...	2011	21991696
trends in nosocomial bloodstream infections in a burn intensive care unit: an eight-year survey.	this study was designed to evaluate the frequency and profile of bloodstream infection (bsi) in a burn intensive care unit (bicu) in tripoli, libya, from 1st january 2000 to 31st december 2007 and to determine the prevalence of different bacteria involved in such infections and their antimicrobial susceptibilities. during the eight-year study period, 995 patients were admitted to the bicu. blood cultures were collected from each septicaemic case and reviewed for age, sex, total body surface area ...	2010	21991204
antimicrobial effect of bee honey in comparison to antibiotics on organisms isolated from infected burns.	despite recent advances in antimicrobial chemotherapy and burn wound management, infection continues to be an important problem in burns. honey is the most famous rediscovered remedy that is used to treat infected wounds and promote healing. the present study aims to evaluate the antimicrobial effect of bee honey on organisms isolated from infected burns in comparison to the antibiotics used in treatment of burn infection, and to evaluate the effects produced when bee honey is added to antibioti ...	2007	21991075
Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiella pneumoniae-specific plasmid associated with a major nosocomial outbreak.	Objectives To characterize the complete sequence, horizontal spread and stability of the CTX-M-15-encoding multiresistance plasmid of a Klebsiella pneumoniae strain involved in a large nosocomial outbreak. Methods The 220 kbp plasmid pUUH239.2 was completely sequenced using 454 technology. The conjugational host range, conjugation frequencies, plasmid stability and fitness cost of plasmid carriage were studied in vitro. Conjugational spread during the outbreak was assessed retrospectively by mu ...	2012	21990049
emergence of klebsiella pneumoniae isolate co-producing ndm-1 with kpc-2 from india.		2012	21990048
Long pentraxin 3 (PTX3) in the light of its structure, mechanism of action and clinical implications.	Pentraxins are a group of evolutionarily conserved ancient proteins. Depending on their structure, pentraxins are divided into short and long pentraxin families. Pentraxin 3 (PTX3) is the prototype of the long pentraxin group. PTX3 synthesis is stimulated by a variety of molecules involved in the inflammatory process. The inflammatory mediator is typically produced at inflammatory sites; however, it can also be released at the sites remote from the original inflammatory insult. Although mainly e ...	2011	21988562
synthesis and biological activity of ethyl 2-(substituted benzylthio)-4-(3'-(ethoxycarbonyl)biphenyl-4-yl)-6-methyl-1,4-dihydropyrimidine-5-carboxylate derivatives.	in the present study, a new series of ethyl 2-(substituted benzylthio)-4-(3'-(ethoxycarbonyl)-biphenyl-4-yl)-6-methyl-1,4-dihydropyrimidine-5-carboxylate derivatives was synthesized. the newly synthesized compounds were characterized by (1) h-nmr, mass and c, h, n analyses. all newly synthesized compounds were screened for their antibacterial (escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes and klebsiella pneumoniae) and antifungal (aspergillus flavus, asp ...	2011	21987259
characterization of a dha-1-producing klebsiella pneumoniae strain involved in an outbreak and role of the ampr regulator in virulence.	a clonal strain of klebsiella pneumoniae producing the plasmid-encoded cephalosporinase dha-1 was isolated from four patients admitted to the teaching hospital of clermont-ferrand, france, in 2006. it was responsible for severe infections in three of the patients; the fourth was colonized only in the gastrointestinal tract. the strain had at least two plasmids encoding resistance to antibiotics (quinolones, aminoglycosides, chloramphenicol, sulfonamides, and trimethoprim), as shown by disk diffu ...	2012	21986829
[hospital and community-acquired β-lactamases-producing escherichia coli and klebsiella pneumoniae at hospitals in hermosillo, sonora].	to determine the prevalence of extended-spectrum β-lactamases (esbl)-producing esherichia coli and klebsiella pneumoniae in hospitals of hermosillo, sonora, mexico.	2011	21986791
Recent trends in antibiotic resistance in European ICUs.	Antimicrobial resistance is an emerging problem in ICUs worldwide. As numbers of published results from national/international surveillance studies rise rapidly, the amount of new information may be overwhelming. Therefore, we reviewed recent trends in antibiotic resistance in ICUs across Europe in the past 18 months.	2011	21986462
extended-spectrum β-lactamase producing klebsiella pneumoniae from blood cultures in puducherry, india.		2011	21985825
asymptomatic bacteriuria among an obstetric population in ibadan.	asymptomatic bacteriuria in pregnancy is the major risk factor for symptomatic urinary tract infection during pregnancy. screening and identification of bacteriuria during pregnancy have been recommended.	2011	21984454
Evaluation of doripenem utilization and susceptibilities at a large urban hospital.	Background Bacterial resistance presents a constant challenge in the treatment of hospitalized patients, particularly with Gram-negative infections. Carbapenems have an important role in the treatment of resistant nosocomial organisms. Doripenem, a recently approved carbapenem, has shown efficacy in clinical trials, but there is little published data on utilization in a general patient population. Objective The clinical utilization of doripenem in a general adult inpatient population was evaluat ...	2011	21984226
Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens.	Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate synthase, 2,3-butanediol dehydrogenase, and a LysR-like regulator, respectively. Unlike in Klebsiella sp. or Klebsiella pneumonie and Vibrio sp. or Vibrio cholerae, the gene slaC i ...	2011	21983710
hemoperfusion with polymyxin b in septic shock secondary to klebsiella pneumoniae induced liver abscesses.		2011	21983271
implementation and outcomes of a hospital-wide computerised antimicrobial stewardship programme in a large medical centre in taiwan.	antibiotic stewardship is important to address the problem of antimicrobial resistance, but a practical and sustainable strategy to provide stewardship in a large hospital setting is lacking. we developed a hospital-wide computerised antimicrobial approval system (hcaas) to guide the use of antimicrobial agents in late 2004 in a 3500-bed medical centre in taiwan. the objective of this study was to evaluate the impacts of hcaas on the hospital from 2003 to 2009. following hcaas deployment, the gr ...	2011	21982143
tannin extracts from immature fruits of terminalia chebula fructus retz. promote cutaneous wound healing in rats.	tannins extracted from immature fruits of terminalia chebula fructus retz. are considered as effective components promoting the process of wound healing. the objective of this study is to explore the optimal extraction and purification technology (oept) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.	2011	21982053
1,3-Dihydro-2H-indol-2-ones derivatives: design, synthesis, in vitro antibacterial, antifungal and antitubercular study.	1,3-dihydro-2H-indol-2-ones derivatives are reported to exhibit a wide variety of biodynamic activities such as antituberculer, anti HIV, fungicidal, antibacterial, anticonvulsant. These valid observations led us to synthesize some new indole-2-one derivative. Thus, herein we report synthesis of various 5-substituted-3-[{5-(6-methyl-2-oxo/thioxo-4-phenyl-1,2,3,4 tetrahydro pyrimidin-5-yl)-1,3,4-thiadiazol-2-yl}imino]-1,3-dihydro-2H-indol-2-one derivatives 4a-l using one pot multicomponent-Bigine ...	2011	21981980
[incidence, risk factors and outcome of nosocomial pneumonia patients with central nervous system infections].	pneumonia is the most frequent nosocomial infection in intensive care units. the reported frequency varies with definition, the type of hospital or intensive care units and the population of patients. the incidence ranges from 6.8-27%.	2011	21980657
plasmid typing and genetic context of ampc β-lactamases in enterobacteriaceae lacking inducible chromosomal ampc genes: findings from a spanish hospital 1999-2007.	to gain insights into ampc transmission between bacterial strains.	2012	21980067
risk factors for outbreaks of multidrug-resistant klebsiella pneumoniae in critical burn patients.	the objective of this study is to identify the risk factors related to colonization or infection in an outbreak of multidrug-resistant klebsiella pneumoniae in a burn patient unit. the authors studied the risk factors associated with colonization or infection using a case-control study design involving patients with multidrug resistant k. pneumoniae (n = 26) and controls (n = 50). they describe the outbreak and provide a retrospective analysis that encompasses patient demographics, microbiologic ...	2011	21979841
prevalence of extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae in food-producing animals.	to evaluate the diversity of extended-spectrum β-lactamases (esbl) genes among food-producing animals, 48 isolates of esbl-producing escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. esbl-carrying e. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. one esbl-producing klebsiella pneumoniae was isolated from a broiler. the esbl-positive e. coli i ...	2011	21979457
Comparative study of antimicrobial activity and phytochemical analysis of methanolic and aqueous extracts of the fruit of Emblica officinalis against pathogenic bacteria.	To investigate antimicrobial potential of aqueous (infusions, decoctions) and methanlic extracts (1:2 and 1:5 concentrations) of Emblica officinalis (amla) against seven pathogenic bacteria namely Staphylococcs aureus, Staphylococcus saprophyticus, Escherichia coli, Enterococcus faecalis, Enterococcus cloacae, Proteus vulgaris and Klebsiella pneumoniae.	2011	21977871
Septic arthritis as a presentation of Crohn's disease.	A 42-year-old man with colonic Crohn's disease presented to the orthopaedic department with septic arthritis of the knee with frank intra-articular pus, which persisted despite repeated joint washouts. This coincided with a symptomatic flare of his colonic Crohns. Imaging revealed a fistulous connection from the proximal sigmoid colon into the pelvis, a psoas abscess, myositis of the musculature around the left pelvic girdle and left thigh and two focal collections almost surrounding the distal ...	2011	21977716
a survey on urinary tract infections associated with the three most common uropathogenic bacteria.	objectives: the most common uropathogenic gram negative bacteria are escherichia coli and klebsiella pneumoniae. the purpose of this study was to determine the three most frequent bacterial agents causing urinary tract infections (uti) in patients who referred to central laboratory of dr. shariati hospital, during 2 years (january 2006- december 2007).materials and methods: the registered data were checked and collected by the questionnaires as a retrospective epidemiological survey. then, the c ...	2010	21977133
a prospective study of catheter-related complications in hpn patients.	background & aims: central venous access device (cvad)-related complications such as catheter-related sepsis, occlusion and breakage contribute to both mortality and morbidity in home parenteral nutrition (hpn) patients. prospectively collected data in these patients are scarce. methods: data on all cvad-related complications in four tertiary referral centres in australia and new zealand were prospectively collected between 01/01/2009 and 31/12/2009 together with costs of each episode that requi ...	2011	21974812
european dissemination of a single oxa-48-producing klebsiella pneumoniae clone.	a klebsiella pneumoniae isolate with decreased susceptibility to carbapenems was isolated in april 2011 in a hospital in amsterdam (the netherlands) and later found to be the source of an important outbreak in a rotterdam hospital. the strain, belonging to sequence type (st) 395, carried the bla(oxa-48) gene located onto a c 62-kb conjugative plasmid, together with the extended-spectrum β-lactamase gene bla(ctx-m-15) . it was closely related or identical to other oxa-48-positive klebsiella pneum ...	2011	21973185
in vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria.	silver carbenes may represent novel, broad-spectrum antimicrobial agents that have low toxicity while providing varying chemistry for targeted applications. here, the bactericidal activity of four silver carbene complexes (sccs) with different formulations, including nanoparticles (nps) and micelles, was tested against a panel of clinical strains of bacteria and fungi that are the causative agents of many skin and soft tissue, respiratory, wound, blood, and nosocomial infections.	2012	21972270
duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization.	epidemiology of celiac disease (cd) is increasing. cd mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. compared to healthy individuals, cd patients seemed to be characterized by higher numbers of gram-negative bacteria and lower numbers gram-positive bacteria.	2011	21970810
predictors of in-hospital mortality in elderly patients with bacteraemia admitted to an internal medicine ward.	abstract:	2011	21970460
bacterial isolates in chronic suppurative otitis media: a changing pattern?	the most frequently isolated organism in chronic suppurative otitis media from different parts of the world is pseudomonas aeruginosa. the pattern from this bacteriological study from our region is different. this study was carried out on 97 patients presenting to the outpatient clinic of the national ear care center, kaduna. nigeria from may 2008 to april 2009. the patients were aged between 1 year and 75 years comprising 50) males and 47 females giving a male to female ratio of 1:1. most of th ...	2011	21970268
the disinfecting potential of contact lens soutions used by sultan qaboos university students.	this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman.	2011	21969898
A cautionary case of microbial solidarity: concurrent isolation of VIM-1-producing Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae from an infected wound.		2012	21969331
Synthesis and antimicrobial properties of naphthylamine derivatives having a thiazolidinone moiety.	The aim of this study was to evaluate the influence of pharmacophores having naphthylamine and nitro groups on the antimicrobial (antibacterial and antifungal) activity of thiazolidinone derivatives.	2011	21968886
molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans.	mastitis, inflammation of the mammary gland, can be caused by a wide range of organisms, including gram-negative and gram-positive bacteria, mycoplasmas and algae. many microbial species that are common causes of bovine mastitis, such as escherichia coli, klebsiella pneumoniae, streptococcus agalactiae and staphylococcus aureus also occur as commensals or pathogens of humans whereas other causative species, such as streptococcus uberis, streptococcus dysgalactiae subsp. dysgalactiae or staphyloc ...	2011	21968538
Comparative effectiveness of aminoglycosides, polymyxin B, and tigecycline for clearance of carbapenem-resistant Klebsiella pneumoniae from urine.	Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an increasingly common cause of health care-associated urinary tract infections. Antimicrobials with in vitro activity against CRKP are typically limited to polymyxins, tigecycline, and often, aminoglycosides. We conducted a retrospective cohort study of cases of CRKP bacteriuria at New York-Presbyterian Hospital from January 2005 through June 2010 to compare microbiologic clearance rates based on the use of polymyxin B, tigecycline, or an ami ...	2011	21968368