Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| quorum sensing-controlled evr regulates a conserved cryptic pigment biosynthetic cluster and a novel phenomycin-like locus in the plant pathogen, pectobacterium carotovorum. | pectobacterium carotovorum scri193 is a phytopathogenic gram-negative bacterium. in this study, we have identified a novel cryptic pigment biosynthetic locus in p. carotovorum scri193 which we have called the pectobacterium orange pigment (pop) cluster. the pop cluster is flanked by two trna genes and contains genes that encode non-ribosomal peptide synthases and polyketide synthase and produces a negatively charged polar orange pigment. orange pigment production is activated when an adjacent tr ... | 2010 | 20192973 |
| complete genome sequence of the fire blight pathogen erwinia amylovora cfbp 1430 and comparison to other erwinia spp. | fire blight, caused by the enterobacterium erwinia amylovora, is a devastating disease of rosaceous plants that has global economic importance for apple and pear production and trade. the complete genome of e. amylovora cfbp 1430 was sequenced, annotated, and compared with the genomes of other erwinia spp. several singleton and shared features of the e. amylovora cfbp 1430 genome were identified that offer a first view into evolutionary aspects within the genus erwinia. comparative genomics iden ... | 2010 | 20192826 |
| the lycopene cyclase crty from pantoea ananatis (formerly erwinia uredovora) catalyzes an fadred-dependent non-redox reaction. | the cyclization of lycopene generates provitamin a carotenoids such as beta-carotene and paves the way toward the formation of cyclic xanthophylls playing distinct roles in photosynthesis and as precursors for regulatory molecules in plants and animals. the biochemistry of lycopene cyclization has been enigmatic, as the previously proposed acid-base catalysis conflicted with the possibility of redox catalysis as predicted by the presence of a dinucleotide binding site. we show that reduced fad i ... | 2010 | 20178989 |
| frequency and diversity of small cryptic plasmids in the genus rahnella. | rahnella is a widely distributed genus belonging to the enterobacteriaceae and frequently present on vegetables. although rahnella has interesting agro-economical and industrial properties and several strains possess antibiotic resistances and toxin genes which might spread within microbial communities, little is known about plasmids of this genus. thus, we isolated a number of rahnella strains and investigated their complements of small plasmids. | 2010 | 20170524 |
| synthesis of amino acid conjugates to 2-imino-3-methylene-5-carboxypyrrolidine and 2-imino-3-methylene-6-carboxypiperidine. | the four stereomers of 2-imino-3-methylene-5-l(carboxy-l-valyl)pyrrolidine, a bacterial metabolite that is inhibitory to the fire blight bacterium erwinia amylovora, were synthesised and compared for antibacterial activity. several alternative amino acid conjugates with l,l-stereochemistry were also prepared, and the synthesis was extended to 3-methylenepiperidine-6-l-carboxylic acid and a selection of 2-imino-3-methylenepiperidine-6-l-carboxy-l-amino acid conjugates. all synthetic amino acid co ... | 2010 | 20167485 |
| floral transmission of erwinia tracheiphila by cucumber beetles in a wild cucurbita pepo. | cucumber beetles, acalymma vittatum (f.) and diabrotica undecipunctata howardi (barber), are specialist herbivores of cucurbits and the vector of erwinia tracheiphila (e.f. smith) holland, the causative agent of wilt disease. cucumber beetles transmit e. tracheiphila when infected frass falls onto leaf wounds at the site of beetle feeding. we show that e. tracheiphila also can be transmitted via the floral nectaries of cucurbita pepo ssp. texana l. andres (texas gourd). under field conditions, w ... | 2010 | 20146850 |
| ion conduction in ligand-gated ion channels: brownian dynamics studies of four recent crystal structures. | four x-ray crystal structures of prokaryotic homologs of ligand-gated ion channels have recently been determined: elic from erwinia chrysanthemi, two structures of a proton-activated channel from gloebacter violaceus (glic1 and glic2) and that of the e221a mutant (glic1m). the availability of numerous structures of channels in this family allows for aspects of channel gating and ion conduction to be examined. here, we determine the likely conduction states of the four structures as well as iv cu ... | 2010 | 20141753 |
| effect of concentration and substrate flow rate on isomaltulose production from sucrose by erwinia sp. cells immobilized in calcium-alginate using packed bed reactor. | isomaltulose was obtained from sucrose solution by immobilized cells of erwinia sp. d12 using a batch and a continuous process. parameters for sucrose conversion into isomaltulose were evaluated using both experimental design and response surface methodology. erwinia sp. d12 cells were immobilized in different alginates, and the influence of substrate flow rate and concentration parameters to produce isomaltulose from sucrose were observed. response surface methodology demonstrated that packed b ... | 2010 | 20135241 |
| hpaxm from xanthomonas citri subsp. malvacearum is a novel harpin with two heptads for hypersensitive response. | a novel harpin-like protein, hpaxm, was described from cotton leaf blight bacteria, xanthomonas. citri subsp. malvacearum. the hpaxm was found to be localized between hrp2 and hrcc. a phylogenetic analysis of the complete amino acid sequence or solely the 13 highly conserved residues h2n-sekqldqlltqli-cooh in the n-terminal alpha-helix indicates that hpaxm is evolutionarily closer to hpagxag and hpaxac than to hpa1xoo and hpa1xoc. a synthesized peptide containing two heptads, 39-ldqlltq-limallq- ... | 2010 | 20134233 |
| complete genome sequence of the plant pathogen erwinia amylovora strain atcc 49946. | erwinia amylovora causes the economically important disease fire blight that affects rosaceous plants, especially pear and apple. here we report the complete genome sequence and annotation of strain atcc 49946. the analysis of the sequence and its comparison with sequenced genomes of closely related enterobacteria revealed signs of pathoadaptation to rosaceous hosts. | 2010 | 20118253 |
| the erwinia amylovora phopq system is involved in resistance to antimicrobial peptide and suppresses gene expression of two novel type iii secretion systems. | the phopq system is a pleiotropic two-component signal transduction system that controls many pathogenic properties in several mammalian and plant pathogens. three different cues have been demonstrated to activate the phopq system including a mild acidic ph, antimicrobial peptides, and low mg(2+). in this study, our results showed that phopq mutants were more resistant to strong acidic conditions (ph 4.5 or 5) than that of the wild-type (wt) strain, suggesting that this system in erwinia amylovo ... | 2010 | 20116983 |
| secretion and translocation signals and dspb/f-binding domains in the type iii effector dspa/e of erwinia amylovora. | dspa/e is a type iii effector of erwinia amylovora, the bacterial pathogen that causes fire blight disease in roseaceous plants. this effector is indispensable for disease development, and it is translocated into plant cells. a dspa/e-specific chaperone, dspb/f, is necessary for dspa/e secretion and possibly for its translocation. in this work, dspb/f-binding sites and secretion and translocation signals in the dspa/e protein were determined. based on yeast two-hybrid assays, dspb/f was found to ... | 2010 | 20110301 |
| therapeutic drug monitoring of asparaginase in the all-bfm 2000 protocol between 2000 and 2007. | on a voluntary basis therapeutic drug monitoring (tdm) was implemented in the all-bfm 2000 protocol for the three currently used asparaginase (asnase) preparations (first line: native escherichia coli asnase; second line: pegylated asnase and third line: erwinia chrysanthemi asnase). | 2010 | 20108339 |
| assessment of inheritance pattern and agronomic performance of transgenic rapeseed having harpin xooc-encoding hrf2 gene. | hrf2 gene is a member of the harpin-encoding gene family of rice-pathogenic bacterium xanthomonas oryzae pv. oryzicola. in our previous studies, we observed that harpin(xooc) could elicit hypersensitive cell death in non-host plants, induce disease and insect resistance in plants, and enhance plant growth. in this study, the rapeseed cultivar, yangyou 4, was genetically engineered via agrobacterium-mediated transformation to express the hrf2 gene. polymerase chain reaction (pcr) and southern blo ... | 2010 | 20107894 |
| regulation of plant glycine decarboxylase by s-nitrosylation and glutathionylation. | mitochondria play an essential role in nitric oxide (no) signal transduction in plants. using the biotin-switch method in conjunction with nano-liquid chromatography and mass spectrometry, we identified 11 candidate proteins that were s-nitrosylated and/or glutathionylated in mitochondria of arabidopsis (arabidopsis thaliana) leaves. these included glycine decarboxylase complex (gdc), a key enzyme of the photorespiratory c(2) cycle in c3 plants. gdc activity was inhibited by s-nitrosoglutathione ... | 2010 | 20089767 |
| molecular cloning and characterization of the novel acidic xylanase xyld from bispora sp. mey-1 that is homologous to family 30 glycosyl hydrolases. | we cloned and sequenced a xylanase gene named xyld from the acidophilic fungus bispora sp. mey-1 and expressed the gene in pichia pastoris. the 1,422-bp full-length complementary dna fragment encoded a 457-amino acid xylanase with a calculated molecular mass of 49.8 kda. the mature protein of xyld showed high sequence similarity to both glycosyl hydrolase (gh) families 5 and 30 but was more homologous to members of gh 30 based on phylogenetic analysis. xyld shared the highest identity (49.9%) wi ... | 2010 | 20077114 |
| [cloning and expressing of a harpin-encoding gene from pseudomonas syringae pv. glycinea]. | methods, objective: we amplified the 1026 bp hrp (hypersensitive response and pathogenicity) gene from pseudomonas syringae pv. glycinea isolate psg12 genomic dna by pcr technique, and then constructed expression vector pgex-hrpz(psg12) with regular molecular cloning operation. the recombinant plasmid was transformed into bl21(de3). recombinant protein was induced by isopropylthio-beta-d-galacgoside (iptg). results: the molecular mass of the fusion protein is 61kda analyzed by sds-page. the prot ... | 2009 | 20069890 |
| [the influence of colonizing methylobacteria on morphogenesis and resistance of sugar beet and white cabbage plants to erwinia carotovora]. | the influence of colonization of sugar beet (beta vulgaris var. saccharifera (alef) krass) and white cabbage (brassica oleracea var. capitata l.) plants by methylotrophic bacteria methylovorus mays on the growth, rooting, and plant resistance to phytopathogen bacteria erwinia carotovora was investigated. the colonization by methylobacteria led to their steady association with the plants which had increased growth speed, root formation and photosynthetic activity. the colonized plants had increas ... | 2009 | 20067151 |
| pantoea gaviniae sp. nov. and pantoea calida sp. nov., isolated from infant formula and an infant formula production environment. | five gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. biochemical tests and partial rpob gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family enterobacteriaceae, closely related to several species of the genera pantoea and erwinia, which indicated a phylogenet ... | 2010 | 20061487 |
| mutations in rpsl that confer streptomycin resistance show pleiotropic effects on virulence and the production of a carbapenem antibiotic in erwinia carotovora. | spontaneous streptomycin-resistant derivatives of erwinia carotovora subsp. carotovora strain attn10 were isolated. sequencing of the rpsl locus (encoding the ribosomal protein s12) showed that each mutant was missense, with a single base change, resulting in the substitution of the wild-type lysine by arginine, threonine or asparagine at codon 43. phenotypic analyses showed that the rpsl mutants could be segregated into two groups: k43r mutants showed reduced production of the beta-lactam secon ... | 2010 | 20056700 |
| genetically pyramiding protease-inhibitor genes for dual broad-spectrum resistance against insect and phytopathogens in transgenic tobacco. | protease inhibitors provide a promising means of engineering plant resistance against attack by insects and pathogens. sporamin (trypsin inhibitor) from sweet potato and cecpi (phytocystatin) from taro were stacked in a binary vector, using pmspoa (a modified sporamin promoter) to drive both genes. transgenic tobacco lines of t0 and t1 generation with varied inhibitory activity against trypsin and papain showed resistance to both insects and phytopathogens. larvae of helicoverpa armigera that in ... | 2010 | 20055959 |
| molecular signature of differential virulence in natural isolates of erwinia amylovora. | abstract erwinia amylovora, the causal agent of fire blight, is considered to be a genetically homogeneous species based on physiological, biochemical, phylogenetic, and genetic analysis. however, e. amylovora strains exhibiting differential virulence are isolated from nature. the exopolysaccharide amylovoran and type iii secretion system (t3ss) are two major yet separate virulence factors in e. amylovora. the objective of this study was to investigate whether there is a correlation between e. a ... | 2010 | 20055653 |
| crystal structure of salt-tolerant glutaminase from micrococcus luteus k-3 in the presence and absence of its product l-glutamate and its activator tris. | glutaminase from micrococcus luteus k-3 [micrococcus glutaminase (mglu); 456 amino acid residues (aa); 48 kda] is a salt-tolerant enzyme. our previous study determined the structure of its major 42-kda fragment. here, using new crystallization conditions, we determined the structures of the intact enzyme in the presence and absence of its product l-glutamate and its activator tris, which activates the enzyme by sixfold. with the exception of a 'lid' part (26-29 aa) and a few other short stretche ... | 2010 | 20050917 |
| comparative genomic and phylogenetic analyses reveal the evolution of the core two-component signal transduction systems in enterobacteria. | the two-component signal transduction system (tcst) consists of a histidine kinase (hk) and a response regulator (rr). tcsts play important roles in sensing and reacting to environmental changes, and in bacterial pathogenesis. previously, we have identified and characterized tcsts in erwinia amylovora, a severe plant enterobacterial pathogen, at genome-wide level. here we conducted a comparative genomic analysis of tcsts in 53 genomes of 16 enterobacterial species. these species include importan ... | 2010 | 20049425 |
| complete genome sequence of the fire blight pathogen erwinia pyrifoliae dsm 12163t and comparative genomic insights into plant pathogenicity. | erwinia pyrifoliae is a newly described necrotrophic pathogen, which causes fire blight on asian (nashi) pear and is geographically restricted to eastern asia. relatively little is known about its genetics compared to the closely related main fire blight pathogen e. amylovora. | 2010 | 20047678 |
| identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (malus x domestica) with erwinia amylovora. | the necrogenic enterobacterium, erwinia amylovora is the causal agent of the fire blight (fb) disease in many rosaceae species, including apple and pear. during the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. no resistance mechanism to e. amylovora in host plants has yet been characterized, recent work has identified some molecular events which occur in resistant and/or susceptible host ... | 2010 | 20047654 |
| dapdiamides, tripeptide antibiotics formed by unconventional amide ligases. | construction of a genomic dna library from pantoea agglomerans strain cu0119 and screening against the plant pathogen erwinia amylovora yielded a new family of antibiotics, dapdiamides a-e (1-5). the structures were established through 2d-nmr experiments and mass spectrometry, as well as the synthesis of dapdiamide a (1). transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. the dapdiamide family's promiscuous biosynthetic pathway contains two uncon ... | 2010 | 20041689 |
| the pmrab system in erwinia amylovora renders the pathogen more susceptible to polymyxin b and more resistant to excess iron. | the pmrab system is a two-component regulatory system that responds to extracellular iron and acidic ph. the role of the pmrab system in erwinia amylovora remains unknown so far. our results showed that the pmrab mutants were more resistant to strong acidic conditions than the wild type (wt) strain. the survival rate of the pmrab mutants was much higher than that of wt when treated with polymyxin b. however, pmrab mutants were more sensitive to extracellular iron than wt strain. these results de ... | 2010 | 20034562 |
| dihydrochalcones: implication in resistance to oxidative stress and bioactivities against advanced glycation end-products and vasoconstriction. | flavonoids are a group of polyphenol compounds with known antioxidant activities. among them, dihydrochalcones are mainly found in apple leaves (malus domestica). glycosylated dihydrochalcones were previously found in large amounts in leaves of two genotypes of malus with contrasting resistance to fire blight, a bacterial disease caused by erwinia amylovora. in the present study we demonstrate that soluble polyphenol patterns comprised phloridzin alone or in combination with two additional dihyd ... | 2010 | 20022617 |
| long-range activation of systemic immunity through peptidoglycan diffusion in drosophila. | the systemic immune response of drosophila is known to be induced both by septic injury and by oral infection with certain bacteria, and is characterized by the secretion of antimicrobial peptides (amps) into the haemolymph. to investigate other possible routes of bacterial infection, we deposited erwinia carotovora (ecc15) on various sites of the cuticle and monitored the immune response via expression of the amp gene diptericin. a strong response was observed to deposition on the genital plate ... | 2009 | 20019799 |
| miniprimer pcr assay targeting multiple genes: a new rapid and reliable tool for genotyping pantoea stewartii subsp. stewartii. | development of a 'miniprimer' pcr assay for genotyping pantoea stewartii subsp. stewartii, the causal agent of the stewart's bacterial wilt on maize. | 2010 | 20002575 |
| erwinia asparaginase: coming closer to an understanding of its use in pediatric acute lymphoblastic leukemia? | 2010 | 19967774 | |
| transgenic apple plants overexpressing the lc gene of maize show an altered growth habit and increased resistance to apple scab and fire blight. | transgenic apple plants (malus x domestica cv. 'holsteiner cox') overexpressing the leaf colour (lc) gene from maize (zea mays) exhibit strongly increased production of anthocyanins and flavan-3-ols (catechins, proanthocyanidins). greenhouse plants investigated in this study exhibit altered phenotypes with regard to growth habit and resistance traits. lc-transgenic plants show reduced size, transversal gravitropism of lateral shoots, reduced trichome development, and frequently reduced shoot dia ... | 2010 | 19967387 |
| structure of helicobacter pylori l-asparaginase at 1.4 a resolution. | bacterial l-asparaginases have been used in the treatment of childhood acute lymphoblastic leukaemia for over 30 years. their therapeutic effect is based on their ability to catalyze the conversion of l-asparagine, an essential amino acid in certain tumours, to l-aspartic acid and ammonia. two l-asparaginases, one from escherichia coli and the other from erwinia chrysanthemi, have been widely employed in clinical practice as anti-leukaemia drugs. however, l-asparaginases are also able to cause s ... | 2009 | 19966411 |
| inhibition by chestnut honey of n-acyl-l-homoserine lactones and biofilm formation in erwinia carotovora, yersinia enterocolitica, and aeromonas hydrophila. | bacteria are able to communicate and coordinate certain processes using small secreted signaling molecules called autoinducers. this phenomenon, known as "quorum sensing" (qs), may be essential for the synchronization of virulence factors as well as biofilm development. the interruption of bacterial qs is acknowledged to attenuate virulence and considered to be a potential new therapy to treat infections caused by pathogenic bacteria. n-acyl-l-homoserine lactones (ahls) have been identified as t ... | 2009 | 19950997 |
| emended description of the genus pantoea, description of four species from human clinical samples, pantoea septica sp. nov., pantoea eucrina sp. nov., pantoea brenneri sp. nov. and pantoea conspicua sp. nov., and transfer of pectobacterium cypripedii (hori 1911) brenner et al. 1973 emend. hauben et al. 1998 to the genus as pantoea cypripedii comb. nov. | bacterial strains belonging to dna hybridization groups (hg) ii, iv and v, in the erwinia herbicola-enterobacter agglomerans complex, of brenner et al. [int j syst bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus pantoea, but have never been formally described and classified. additionally, it has been shown in several studies that pectobacterium cypripedii is more closely related to species of pantoea than to those of pectobacterium. in this study, the phylogenetic po ... | 2010 | 19946052 |
| [phytopathogenic bacteria of couch-grass in the crops of wheat]. | bacterialdiseases of weeds in the crops of wheat on the fields of kyiv and vinnytsya regions of ukraine elytrigia repens (l.) nevski agropyrum repens l. were revealed. the following symptoms of bacterial affections: the leaves wither, oval or hatched necrotic spots on green leaves, necroses on the stalks, empty-ears, partial blackening of the ear axes, awns, caryopsises, scales, water-soaked or dark brown with violet shade spots on the rhizomes were found. during the vegetation period bacteria w ... | 2009 | 19938603 |
| [the characteristic of the colispecific bacteriocins of erwinia carotovora subsp. carotovora ec153]. | it has been shown for the first time that macromolecular carotovoricins from erwinia carotovora subsp. carotovora ec153 strain are active to escherichia coli and posses endonuclease activity. it has been established that 19.1% of strains isolated from the patients are sensitive to these bacteriocins. the type and the form of these particles have been also determined and they show resemblance with phage objects. | 2009 | 19938602 |
| characterization of an endophytic bacterial community associated with eucalyptus spp. | endophytic bacteria were isolated from stems of eucalyptus spp (eucalyptus citriodora, e. grandis, e. urophylla, e. camaldulensis, e. torelliana, e. pellita, and a hybrid of e. grandis and e. urophylla) cultivated at two sites; they were characterized by rapd and amplified rdna restriction analysis (ardra). endophytic bacteria were more frequently isolated from e. grandis and e. pellita. the 76 isolates were identified by 16s rdna sequencing as erwinia/pantoea (45%), agrobacterium sp (21%), curt ... | 2009 | 19937585 |
| the effects of extracellular adenosine 5'-triphosphate on the tobacco proteome. | extracellular adenosine 5'-triphosphate (eatp) is emerging as an important plant signalling compound capable of mobilising intracellular second messengers such as ca(2+), nitric oxide, and reactive oxygen species. however, the downstream molecular targets and the spectrum of physiological processes that eatp regulates are largely unknown. we used exogenous atp and a non-hydrolysable analogue as probes to identify the molecular and physiological effects of eatp-mediated signalling in tobacco. 2-d ... | 2010 | 19899079 |
| transgenic amorphophallus konjac expressing synthesized acyl-homoserine lactonase (aiia) gene exhibit enhanced resistance to soft rot disease. | amorphophallus konjac is an important economic crop widely used in health products and biomaterials. however, this monocotyledonous plant's production is seriously restricted by soft rot disease. some bacillus thuringiensis strains generate an endocellular acyl homoserine lactonase (aiia), which has inhibitory effect on soft rot pathogen through disrupting the signal molecules (n-acylhomoserine lactones, ahl) of their quorum sensing system. the aim of our study is to obtain transgenic a. konjac ... | 2009 | 19898849 |
| novel low-temperature-active phytase from erwinia carotovora var.carotovota accc 10276. | a phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. therefore, this study used a degenerate pcr and tail pcr to clone a phytase gene from erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. the full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kda with a 50% amino acid identity to the klebsiella pneumoniae phytase ... | 2009 | 19884763 |
| unique features of erwinia chrysanthemi (dickeya dadantii) ra3b genes involved in the blue indigoidine production. | erwinia chrysanthemi (ech) ra3b produces a large amount of blue indigoidine. using tn5-induced mutagenesis, three indigoidine-deficient mutants were generated. followed by library screening, a 5.8kb fragment complemented mutants for indigoidine synthesis was cloned. this fragment contains four complete open-reading frames (orfs), pecs, pecm, idga, and idgb, and two partial orfs, argg, and idgc. these genes are nearly identical to those in strain ech3937. primer extension assays demonstrated a cl ... | 2010 | 19879116 |
| linking environmental heterogeneity and reproductive success at single-cell resolution. | individual-based microbial ecology (ibme) is a developing field of study in need of experimental tools to quantify the individual experience and performance of microorganisms in their natural habitats. we describe here the conception and application of a single-cell bioreporter approach with broad utility in ibme. it is based on the dilution of stable green fluorescent protein (gfp) in dividing bacteria. in the absence of de novo synthesis, gfp fluorescence of a daughter cell approximates half o ... | 2010 | 19865185 |
| isolation and characterization of aniline degradation slightly halophilic bacterium, erwinia sp. strain hsa 6. | the isolated strain hsa6 is classified as erwinia amylovora based on 16s rdna sequence and the morphological and physiological properties. strain hsa6 is the first reported e. amylovora in pure culture growing with aniline as sole electron donor and carbon source. the suitable ph for strain hsa6 is wide (from 5 to 11). strain hsa6 is slightly halophilic with growth occurring at 0-10% (v/v) nacl, and the suitable nacl concentration for strain hsa6 is from 0% to 6%. the number of bacteria appeared ... | 2010 | 19850455 |
| identification and characterization of three novel esai/esar quorum-sensing controlled stewartan exopolysaccharide biosynthetic genes in pantoea stewartii ssp. stewartii. | 2009 | 19843225 | |
| harpins and ion channels modulations: many ways to die. | harpins are type three secretion system (ttss) effectors. while few harpins are thought to be translocators of ttss effectors through the host plasma membrane during plant/bacteria interactions, functions of many harpins remain for the moment mysterious. we recently showed that the hrpw(ea) harpin from erwinia amylovora, at subnamolar concentration, was able to decrease defense responses triggered by another harpin from this bacteria, hrpn(ea). this antagonism could be the result of opposed anio ... | 2008 | 19841656 |
| determination and confirmation of nicotinic acid and its analogues and derivates in pear and apple blossoms using high-performance liquid chromatography-diode array-electrospray ionization mass spectrometry. | erwinia amylovora causes fire blight, a serious disease of apple and pear. the bacterial pathogen colonizes the flower stigma and hypanthium, where it multiplies and then invades through natural openings (nectarthodes). e. amylovora requires nicotinic acid as growth factor, and competition for nicotinic acid is being explored as a novel biocontrol strategy. the ability of e. amylovora to substitute nicotinic acid with analogues or derivates as growth factors has not been investigated yet. furthe ... | 2009 | 19835358 |
| kunitz trypsin inhibitor: an antagonist of cell death triggered by phytopathogens and fumonisin b1 in arabidopsis. | programmed cell death (pcd) is a central regulatory process in both plant development and in plant responses to pathogens. pcd requires a coordinate activation of pro-apoptotic factors such as proteases and suppressors inhibiting and modulating these processes. in plants, various caspase-like cysteine proteases as well as serine proteases have been implicated in pcd. here, we show that a serine protease (kunitz trypsin) inhibitor (kti1) of arabidopsis acts as a functional kti when produced in ba ... | 2008 | 19825555 |
| contribution of erwinia amylovora exopolysaccharides amylovoran and levan to biofilm formation: implications in pathogenicity. | erwinia amylovora is a highly virulent, necrogenic, vascular pathogen of rosaceous species that produces the exopolysaccharide amylovoran, a known pathogenicity factor, and levan, a virulence factor. an in vitro crystal violet staining and a bright-field microscopy method were used to demonstrate that e. amylovora is capable of forming a biofilm on solid surfaces. amylovoran and levan production deletion mutants were used to determine that amylovoran was required for biofilm formation and that l ... | 2009 | 19821727 |
| thermal stabilization of erwinia chrysanthemi pectin methylesterase a for application in a sugar beet pulp biorefinery. | directed evolution approaches were used to construct a thermally stabilized variant of erwinia chrysanthemi pectin methylesterase a. the final evolved enzyme has four amino acid substitutions that together confer a t(m) value that is approximately 11 degrees c greater than that of the wild-type enzyme, while maintaining near-wild-type kinetic properties. the specific activity, with saturating substrate, of the thermally stabilized enzyme is greater than that of the wild-type enzyme when both are ... | 2009 | 19820151 |
| 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte pantoea agglomerans 48b/90. | the epiphyte pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the enterobacteriaceae, as does the plant pathogen erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. since p. agglomerans efficiently antagonizes phytopathogenic bacteria, the p. agglomerans strain c9-1 is used as a biocontrol agent (blightban c9-1). here we describe the bioassay-guided isolation of a peptide antibiotic that is highly ... | 2009 | 19820144 |
| bacterial chemoattraction towards jasmonate plays a role in the entry of dickeya dadantii through wounded tissues. | jasmonate is a key signalling compound in plant defence that is synthesized in wounded tissues. in this work, we have found that this molecule is also a strong chemoattractant for the phythopathogenic bacteria dickeya dadantii (ex-erwinia chysanthemi). jasmonic acid induced the expression of a subset of bacterial genes possibly involved in virulence/survival in the plant apoplast and bacterial cells pre-treated with jasmonate showed increased virulence in chicory and saintpaulia leaves. we also ... | 2009 | 19818025 |
| genotypic comparison of pantoea agglomerans plant and clinical strains. | pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. however, commercial registration of p. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (bl2) organism due to clinical reports as an opportunistic human pathogen. this study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic ... | 2009 | 19772624 |
| [use of the gene of antimicrobial peptide cecropin p1 for producing marker-free transgenic plants]. | the marker-free transgenic tobacco plants carrying a synthetic gene encoding the antimicrobial peptide cecropin p1 (cecp1) under the control of the cauliflower mosaic virus 35s rna promoter were produced. the binary vector pbm, free of any selective genes of resistance to antibiotics or herbicides intended for selecting transgenic plants, was used for transformation. the transformants were screened on a nonselective medium by detecting cecropin p1 in plant cells according to the antibacterial ac ... | 2009 | 19769295 |
| the olive fly endosymbiont, "candidatus erwinia dacicola," switches from an intracellular existence to an extracellular existence during host insect development. | as polyphagous, holometabolous insects, tephritid fruit flies (diptera: tephritidae) provide a unique habitat for endosymbiotic bacteria, especially those microbes associated with the digestive system. here we examine the endosymbiont of the olive fly [bactrocera oleae (rossi) (diptera: tephritidae)], a tephritid of great economic importance. "candidatus erwinia dacicola" was found in the digestive systems of all life stages of wild olive flies from the southwestern united states. pcr and micros ... | 2009 | 19767463 |
| the dlt operon of bacillus cereus is required for resistance to cationic antimicrobial peptides and for virulence in insects. | the dlt operon encodes proteins that alanylate teichoic acids, the major components of cell walls of gram-positive bacteria. this generates a net positive charge on bacterial cell walls, repulsing positively charged molecules and conferring resistance to animal and human cationic antimicrobial peptides (amps) in gram-positive pathogenic bacteria. amps damage the bacterial membrane and are the most effective components of the humoral immune response against bacteria. we investigated the role of t ... | 2009 | 19767427 |
| biological properties of the chilean native moss sphagnum magellanicum. | an ethanol extract prepared from the gametophyte chilean native moss sphagnum magellanicum was dried out, weighed and dissolved in distilled water. this extract was then assayed for its antibacterial activity against the g(-) bacteria azotobacter vinelandii, erwinia carotovora subsp. carotovora, enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, salmonella typhi, vibrio cholerae, and the g(+) bacteria staphylococcus aureus subsp. aureus, and streptococcus type beta. the growth of ... | 2009 | 19746269 |
| structures of the arm-type binding domains of hpi and hai7 integrases. | the structures of the n-terminal domains of two integrases of closely related but not identical asn tdna-associated genomic islands, yersinia hpi (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an erwinia carotovora genomic island with yet unknown function, hai7, have been resolved. both integrases utilize a novel four-stranded beta-sheet dna-binding motif, in contrast to the known proteins that bind their dna targets by means of three-stranded bet ... | 2009 | 19737930 |
| functional analysis of the n terminus of the erwinia amylovora secreted effector dspa/e reveals features required for secretion, translocation, and binding to the chaperone dspb/f. | dspa/e is a type iii secreted effector protein required for pathogenicity in the apple and pear pathogen erwinia amylovora, and dspb/f is a small chaperone protein involved in dspa/e secretion. while the secretion and translocation signals of many type iii secretion effector proteins in human enteric pathogens have been characterized extensively, relatively little is known about the translocation requirements of many effectors in plant pathogens, including large dspe-like proteins. in this study ... | 2009 | 19737101 |
| catabolism of raffinose, sucrose, and melibiose in erwinia chrysanthemi 3937. | erwinia chrysanthemi (dickeya dadantii) is a plant pathogenic bacterium that has a large capacity to degrade the plant cell wall polysaccharides. the present study reports the metabolic pathways used by e. chrysanthemi to assimilate the oligosaccharides sucrose and raffinose, which are particularly abundant plant sugars. e. chrysanthemi is able to use sucrose, raffinose, or melibiose as a sole carbon source for growth. the two gene clusters scrkyabr and rafrba are necessary for their catabolism. ... | 2009 | 19734309 |
| degradation of pathogen quorum-sensing molecules by soil bacteria: a preventive and curative biological control mechanism. | abstract the plasmid pme6863, carrying the aiia gene from the soil bacterium bacillus sp. a24 that encodes a lactonase enzyme able to degrade n-acyl-homoserine lactones (ahls), was introduced into the rhizosphere isolate pseudomonas fluorescens p3. this strain is not an effective biological control agent against plant pathogens. the transformant p. fluorescens p3/pme6863 acquired the ability to degrade ahls. in planta, p. fluorescens p3/pme6863 significantly reduced potato soft rot caused by erw ... | 2003 | 19719608 |
| [identification and characterization of non-cultivated forms of enterobacteria erwinia carotovora in continuously incubated cultures]. | to determine overall number as well as number of viable cells in continuously incubated cultures of e. carotovora by methods of confocal microscopy and quantitative pcr-analysis. | 2009 | 19715209 |
| identification of a conserved chromosomal region encoding klebsiella pneumoniae type 1 and type 3 fimbriae and assessment of the role of fimbriae in pathogenicity. | type 3 fimbriae are expressed by most clinical klebsiella pneumoniae isolates and mediate adhesion to host structures in vitro. however, the role of type 3 fimbriae in k. pneumoniae virulence has not been evaluated by use of in vivo infection models. in this study, the type 3 fimbrial gene cluster (mrk) of the clinical isolate c3091 is described in detail. the mrk gene cluster was revealed to be localized in close proximity to the type 1 fimbrial gene cluster. thus, a 20.4-kb fimbria-encoding re ... | 2009 | 19703972 |
| expression of phytoene synthase1 and carotene desaturase crti genes result in an increase in the total carotenoids content in transgenic elite wheat (triticum aestivum l.). | dietary micronutrient deficiencies, such as the lack of vitamin a, are a major source of morbidity and mortality worldwide. carotenoids in food can function as provitamin a in humans, while grains of chinese elite wheat cultivars generally have low carotenoid contents. to increase the carotenoid contents in common wheat endosperm, transgenic wheat has been generated by expressing the maize y1 gene encoding phytoene synthase driven by a endosperm-specific 1dx5 promoter in the elite wheat (triticu ... | 2009 | 19694433 |
| tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute. | the antioxidant and antibacterial activities, and total phenolic contents of rosa damascena mill. flower extracts (absolute, essential oil and hydrosol) were investigated. the chemical compositions of these extracts were analysed by gc-ms. phenylethyl alcohol (78.38%) was found to be the main constituent of rose absolute, while citrenellol and geraniol were the major compounds (>55%) of rose essential oil and hydrosol. tocopherol and carotene levels were determined by high performance liquid chr ... | 2009 | 19688375 |
| expression and functional characterization of two pathogenesis-related protein 10 genes from zea mays. | a novel pr10 gene (zmpr10.1) was isolated from maize and its expression and function were compared with the previous zmpr10. zmpr10.1 shares 89.8% and 85.7% identity to zmpr10 at the nucleotide and amino acid sequence level, respectively. zmpr10 and zmpr10.1 were mainly expressed in root tissue with low expression in other tissues. zmpr10.1 had significantly lower expression than zmpr10 in all tissues examined. the expression of both zmpr10 and zmpr10.1 was induced by most abiotic stresses inclu ... | 2010 | 19682768 |
| exploitation of a new flagellatropic phage of erwinia for positive selection of bacterial mutants attenuated in plant virulence: towards phage therapy. | to isolate and characterize novel bacteriophages for the phytopathogen, erwinia carotovora ssp. atroseptica (eca), and to isolate phage-resistant mutants attenuated in virulence. | 2010 | 19674185 |
| erwinia asparaginase after allergy to e. coli asparaginase in children with acute lymphoblastic leukemia. | escherichia coli asparaginase is an important component of treatment for childhood acute lymphoblastic leukemia (all); however, hypersensitivity develops in up to 30% of patients. we assessed the nadir enzyme activity and tolerability of erwinia asparaginase, an alternative preparation, in e. coli asparaginase-allergic patients. | 2010 | 19672973 |
| biophysical investigations on the aggregation and thermal unfolding of harpin(pss) and identification of leucine-zipper-like motifs in harpins. | harpins are heat-stable, glycine-rich proteins secreted by gram-negative bacteria, which induce hypersensitive response (hr) in non-host plants. in this study, the thermal unfolding and aggregation of harpin(pss) from pseudomonas syringae pv. syringae have been investigated by biophysical approaches. differential scanning calorimetric studies indicate that thermal unfolding of harpin(pss) is a complex process involving three distinct transitions. cd spectroscopy revealed that the secondary struc ... | 2009 | 19665594 |
| improve bioavailability of harpin protein on plant use plga based nanoparticle. | harpins can induce systemic acquired resistance (sar) pathway on scores of non-host plant, provide protection against a range of pathogens. in this study, we demonstrated that applied recombinant harpinz pseudomonas syringae pv. tomato (rhrpz) on tobacco with three kinds of methods: infiltrating from micro-pore into leaf; injecting into petiole, and spraying on leaf, there is great difference in assimilation of protein because of the poor osmosis of tobacco leaves, and with multi-application of ... | 2009 | 19665501 |
| phylogenetic evidence for extensive horizontal gene transfer of type iii secretion system genes among enterobacterial plant pathogens. | this study uses sequences from four genes, which are involved in the formation of the type iii secretion apparatus, to determine the role of horizontal gene transfer in the evolution of virulence genes for the enterobacterial plant pathogens. sequences of erwinia, brenneria, pectobacterium, dickeya and pantoea were compared (a) with one another, (b) with sequences of enterobacterial animal pathogens, and (c) with sequences of plant pathogenic gamma and beta proteobacteria, to evaluate probable p ... | 2009 | 19643761 |
| endophytic bacteria associated with growing shoot tips of banana (musa sp.) cv. grand naine and the affinity of endophytes to the host. | a cultivation-based assessment of endophytic bacteria present in deep-seated shoot tips of banana suckers was made with a view to generate information on the associated organisms, potential endophytic contaminants in tissue-cultured bananas and to assess if the endophytes shared a beneficial relationship with the host. plating the tissue homogenate from the central core of suckers showed colony growth on nutrient agar from just 75% and 42% of the 12 stocks during may and november, respectively ( ... | 2009 | 19633807 |
| mutagenesis and functional characterization of the rna and protein components of the toxin abortive infection and toxin-antitoxin locus of erwinia. | bacteria are constantly challenged by bacteriophage (phage) infection and have developed multiple adaptive resistance mechanisms. these mechanisms include the abortive infection systems, which promote "altruistic suicide" of an infected cell, protecting the clonal population. a cryptic plasmid of erwinia carotovora subsp. atroseptica, peca1039, has been shown to encode an abortive infection system. this highly effective system is active across multiple genera of gram-negative bacteria and agains ... | 2009 | 19633081 |
| evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations. | glycosyl hydrolase family 4 (gh4) is exceptional among the 114 families in this enzyme superfamily. members of gh4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site. of greatest significance is the fact that members of gh4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. by phylogenetic analysis, and from available substrate specificities, we have assigned a majority of the enzymes of gh4 to five subgroups. ... | 2009 | 19625389 |
| characterization of homologs of the small rna sgrs reveals diversity in function. | sgrs is a small rna (srna) that requires the rna chaperone hfq for its function. sgrs is a unique dual-function srna with a base pairing function that regulates mrna targets and an mrna function that allows production of the 43-amino-acid protein sgrt. sgrs is expressed when non-metabolizable sugars accumulate intracellularly (glucose-phosphate stress) and is required to allow escherichia coli cells to recover from stress. in this study, homologs of sgrs were used to complement an e. coli sgrs m ... | 2009 | 19620214 |
| using trap crops for control of acalymma vittatum (coleoptera: chrysomelidae) reduces insecticide use in butternut squash. | striped cucumber beetle, acalymma vittatum f., is the primary insect pest of cucurbit crops in the northeastern united states. adult beetles colonize squash crops from field borders, causing feeding damage at the seedling stage and transmitting bacterial wilt erwinia tracheiphila hauben et al. 1999. conventional control methods rely on insecticide applications to the entire field, but surrounding main crops with a more attractive perimeter could reduce reliance on insecticides. a. cittatum shows ... | 2009 | 19610425 |
| enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin a conversion. | carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. in addition to being potent antioxidants, they also provide the vitamin a precursor beta-carotene. in tomato (solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplasts. how the carotenoid biosynthetic pathway is regulated and what limits total carotenoid accumulation in fruit chromoplasts is not well understood. here, we have introduced the lyc ... | 2009 | 19587100 |
| production of l-dopa and dopamine in recombinant bacteria bearing the vitreoscilla hemoglobin gene. | given the well-established beneficial effects of vitreoscilla hemoglobin (vhb) on heterologous organisms, the potential of this protein for the production of l-dopa and dopamine in two bacteria, citrobacter freundii and erwinia herbicola, was investigated. the constructed recombinants bearing the vhb gene (vgb(+)) had substantially higher levels of cytoplasmic l-dopa (112 mg/l for c. freundii and 97 mg/l for e. herbicola) than their respective hosts (30.4 and 33.8 mg/l) and the vgb(-) control st ... | 2009 | 19585534 |
| design, synthesis and in vitro antibacterial/antifungal evaluation of novel 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7(1-piperazinyl)quinoline-3-carboxylic acid derivatives. | a series of 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7(1-piperazinyl)quinoline-3-carboxylic acid (norfloxacin) derivatives were prepared according to the principle of combinating bioactive substructures and tested for their activities against five plant pathogenic bacteria and three fungi in vitro. the preliminary bioassays indicated that almost all synthesized target compounds retained the antibacterial activities of norfloxacin and had some antifungal activities as carboxylic acid amide compounds. t ... | 2009 | 19560843 |
| bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae). | the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ... | 2009 | 19543937 |
| evaluation of antineoplastic activity of extracellular asparaginase produced by isolated bacillus circulans. | l-asparaginase is an important component in the treatment of acute lymphoblastic leukemia in children. its antineoplastic activity toward malignant cells is due to their characteristic nature in slow synthesis of l-asparagine (asn), which causes starvation for this amino acid, while normal cells are protected from asn starvation due to their ability to produce this amino acid. the relative selectivity with regard to the metabolism of malignant cells forces to look for novel asparaginase with lit ... | 2010 | 19543838 |
| slya, a marr family transcriptional regulator, is essential for virulence in dickeya dadantii 3937. | slya, a marr family transcriptional regulator, controls an assortment of biological functions in several animal-pathogenic bacteria. in order to elucidate the functions of slya in the phytopathogen dickeya dadantii (formerly erwinia chrysanthemi) 3937, a slya gene deletion mutant (denoted deltaslya) was constructed. the mutant exhibited increased sensitivity to sodium hypochlorite, the cationic antimicrobial peptide polymyxin b, and oxidative stress. the mutant showed reduced production of pecta ... | 2009 | 19542281 |
| a dyad of lymphoblastic lysosomal cysteine proteases degrades the antileukemic drug l-asparaginase. | l-asparaginase is a key therapeutic agent for treatment of childhood acute lymphoblastic leukemia (all). there is wide individual variation in pharmacokinetics, and little is known about its metabolism. the mechanisms of therapeutic failure with l-asparaginase remain speculative. here, we now report that 2 lysosomal cysteine proteases present in lymphoblasts are able to degrade l-asparaginase. cathepsin b (ctsb), which is produced constitutively by normal and leukemic cells, degraded asparaginas ... | 2009 | 19509471 |
| phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system. | the aim of the present work was to characterize the heterotrophic bacterial community of a marine recirculating aquaculture system (ras). | 2009 | 19508294 |
| erwinia amylovora strains isolated in romania from outbreaks of fire blight disease: phenotypic characterization. | the fire blight disease was described for the first time in romania, in 1992. since then by continuous spreading, this disease has caused severe damages of the fruit trees production, particularly of the pear and quince orchards in different regions of the country, being advantaged by certain weather conditions (high temperatures and humidity). an epidemiological surveillance of this disease that was spreading over different regions of the country, has been instituted since 2002. during the year ... | 2008 | 19496475 |
| molecular-dynamics simulations of elic-a prokaryotic homologue of the nicotinic acetylcholine receptor. | the ligand-gated ion channel from erwinia chrysanthemi (elic) is a prokaryotic homolog of the eukaryotic nicotinic acetylcholine receptor (nachr) that responds to the binding of neurotransmitter acetylcholine and mediates fast signal transmission. elic is similar to the nachr in its primary sequence and overall subunit organization, but despite their structural similarity, it is not clear whether these two ligand-gated ion channels operate in a similar manner. further, it is not known to what ex ... | 2009 | 19486673 |
| comparative genomics-guided loop-mediated isothermal amplification for characterization of pseudomonas syringae pv. phaseolicola. | to design and evaluate a loop-mediated isothermal amplification (lamp) protocol by combining comparative genomics and bioinformatics for characterization of pseudomonas syringae pv. phaseolicola (psp), the causal agent of halo blight disease of bean (phaseolus vulgaris l.). | 2009 | 19486391 |
| systems level analysis of two-component signal transduction systems in erwinia amylovora: role in virulence, regulation of amylovoran biosynthesis and swarming motility. | two-component signal transduction systems (tcsts), consisting of a histidine kinase (hk) and a response regulator (rr), represent a major paradigm for signal transduction in prokaryotes. tcsts play critical roles in sensing and responding to environmental conditions, and in bacterial pathogenesis. most tcsts in erwinia amylovora have either not been identified or have not yet been studied. | 2009 | 19470164 |
| the role of respiratory burst oxidase homologues in elicitor-induced stomatal closure and hypersensitive response in nicotiana benthamiana. | active oxygen species (aos) are central components of the defence reactions of plants against pathogens. plant respiratory burst oxidase homologues (rboh) of gp91(phox), a plasma membrane protein of the neutrophil nicotinamide adenine dinucleotide phosphate (nadph) oxidase, play a prominent role in aos production. the role of two rboh from nicotiana benthamiana, nbrboha and nbrbohb that encode plant nadph oxidase in the process of elicitor-induced stomatal closure and hypersensitive cell death i ... | 2009 | 19454596 |
| microbial siderophores exert a subtle role in arabidopsis during infection by manipulating the immune response and the iron status. | siderophores (ferric ion chelators) are secreted by organisms in response to iron deficiency. the pathogenic enterobacterium erwinia chrysanthemi produces two siderophores, achromobactin and chrysobactin (cb), which are required for systemic dissemination in host plants. previous studies have shown that cb is produced in planta and can trigger the up-regulation of the plant ferritin gene atfer1. to further investigate the function of cb during pathogenesis, we analyzed its effect in arabidopsis ... | 2009 | 19448037 |
| rsmc of erwinia carotovora subsp. carotovora negatively controls motility, extracellular protein production, and virulence by binding flhd and modulating transcriptional activity of the master regulator, flhdc. | rsmc and flhdc are global regulators controlling extracellular proteins/enzymes, rsmb rna, motility, and virulence of erwinia carotovora subsp. carotovora. flhdc, the master regulator of flagellar genes, controls these traits by positively regulating gaca, flia, and rsmc and negatively regulating hexa. rsmc, on the other hand, is a negative regulator of extracellular proteins/enzymes, motility, and virulence since the deficiency of rsmc in flhdc(+) strain results in overproduction of extracellul ... | 2009 | 19447906 |
| small rna identification in enterobacteriaceae using synteny and genomic backbone retention ii. | small rnas are bacterial counterparts of noncoding rnas. increasing evidence being added in the literature indicates that these small rnas play major roles in prokaryotes both at the transcriptome and proteome levels. based on comparative genomic studies, we present manually curated small rna regions in 25 recently completed genomes from enterobacteriaceae. the study is a continuation of our earlier work that uses the presence of small rnas sandwiched between specific conserved flanking genes re ... | 2009 | 19445646 |
| first report on the presence of fire blight resistance in linkage group 11 of pyrus ussuriensis maxim. | fire blight, caused by the gram-negative bacterium erwinia amylovora (burrill) winslow et al., is a dangerous disease of pome fruits, including pear. a pear breeding program for fire blight resistance was initiated in 2003 at the department of pomology, warsaw university of life sciences, poland. since several asian species are considered to be potential sources of resistance to fire blight, the susceptible pyrus communis 'doyenne du comice' was crossed with the resistant p. ussuriensis. the f1 ... | 2009 | 19433906 |
| hyperproduction of 3,4-dihydroxyphenyl-l-alanine (l-dopa) using erwinia herbicola cells carrying a mutant transcriptional regulator tyrr. | in the last few decades, enzymatic production of 3,4-dihydroxyphenyl-l-alanine (l-dopa) using tyrosine phenol-lyase (tpl) has been industrialized. this method has an intrinsic problem of tyrosine contamination because tpl is synthesized under tyrosine-induced conditions. herein, we constructed a hyper-l-dopa-producing strain by exploiting a mutant tyrr, an activator of tpl. the highest productivity was obtained for the strain grown under non-induced conditions. it was 30-fold higher than that ob ... | 2009 | 19420686 |
| does distant homology with evf reveal a lipid binding site in bacillus thuringiensis cytolytic toxins? | the cry and cyt classes of insecticidal toxins derived from the sporulating bacterium bacillus thuringiensis are valuable substitutes for synthetic pesticides in agricultural contexts. crystal structures and many biochemical data have provided insights into their molecular mechanisms, generally thought to involve oligomerization and pore formation, but have not localised the site on cyt toxins responsible for selective binding of phospholipids containing unsaturated fatty acids. here, distant ho ... | 2009 | 19409387 |
| construction and analysis of pathogenicity island deletion mutants of erwinia amylovora. | an easy gene-knockout technique, pcr-based one-step inactivation of chromosomal genes, is widely used in escherichia coli and related enterobacteria to construct mutants. in this study, we adapted this technique to construct genomic island and large operon deletion mutants of erwinia amylovora, including the 33.4 kb hrp-type iii secretion (t3ss) pathogenicity island (pai1) and the 15.8 kb amylovoran biosynthesis (ams) operon. deletion of 2 novel t3ss pathogenicity islands (pai2 and pai3) and an ... | 2009 | 19396246 |
| insect frass as a pathway for transmission of bacterial wilt of cucurbits. | insects that vector diseases of plants are of critical concern to agriculture, but relationships between the vectors and pathogens often are poorly understood. in this study, we present research on vector relationships between the striped cucumber beetle, acalymma vittatum (f.) (coleoptera: chrysomelidae), and the pathogen that causes bacterial wilt of cucurbits, erwinia tracheiphila (smith) (enterobacteriales: enterobacteriaceae). we studied how the bacteria were retained in the gut of the beet ... | 2009 | 19389288 |
| the genome and proteome of a virulent escherichia coli o157:h7 bacteriophage closely resembling salmonella phage felix o1. | based upon whole genome and proteome analysis, escherichia coli o157:h7-specific bacteriophage (phage) wv8 belongs to the new myoviral genus, "the felix o1-like viruses" along with salmonella phage felix o1 and erwinia amylovora phage phiea21-4. the genome characteristics of phage wv8 (size 88.49 kb, mol%g+c 38.9, 138 orfs, 23 trnas) are very similar to those of phage felix o1 (86.16 kb, 39.0 mol%g+c, 131 orfs and 22 trnas) and, indeed most of the proteins have their closest homologs within feli ... | 2009 | 19379502 |
| novel succinylated and large-sized osmoregulated periplasmic glucans of pseudomonas syringae pv. syringae. | osmoregulated periplasmic glucans (opgs) are intrinsic components of the gram-negative bacterial envelope and are important for bacterial-host interactions. the opgs of pseudomonas syringae pv. syringae have been known to be highly branched linear glucans ranging from 6 to 13 glucose residues devoid of any substituents, while having backbone structure similar to those of escherichia coli and erwinia chrysanthemi. here, we report for the first time succinylated and large-sized opgs from p. syring ... | 2009 | 19358981 |