Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| zika virus infection and guillain-barré syndrome in three patients from suriname. | we present three patients from suriname who were diagnosed with guillain-barré syndrome (gbs) during the zika virus (zikv) outbreak in this country. one patient had a positive zikv urine real-time rt-pcr (qrt-pcr) result. the other two patients had a negative zikv urine qrt-pcr but a positive virus neutralization test and presence of igg antibodies against zikv in the serum. considering the evidence of a past zikv infection and absence of evidence for recent infections with the most common prece ... | 2016 | 28066317 |
| campylobacter coli infection in pet birds in southern italy. | avian species are considered as the main reservoir of campylobacter spp. however, few data are available on the presence of this microorganism in pet birds. this study was therefore performed to determine the prevalence of campylobacter spp. in pet birds bred in southern italy. faecal samples were collected from 88 cages housing different species of pet birds and examined by bacteriological culture and polymerase chain reaction. a total of 13.6% of the cage samples were positive for campylobacte ... | 2017 | 28061877 |
| differential roles of iron storage proteins in maintaining the iron homeostasis in mycobacterium tuberculosis. | ferritins and bacterioferritins are iron storage proteins that represent key players in iron homeostasis. several organisms possess both forms of ferritins, however, their relative physiological roles are less understood. mycobacterium tuberculosis possesses both ferritin (bfrb) and bacterioferritin (bfra), playing an essential role in its pathogenesis as reported by us earlier. this study provides insights into the role of these two proteins in iron homeostasis by employing m. tuberculosis bfr ... | 2017 | 28060867 |
| insights into the mechanistic basis of plasmid-mediated colistin resistance from crystal structures of the catalytic domain of mcr-1. | the polymixin colistin is a "last line" antibiotic against extensively-resistant gram-negative bacteria. recently, the mcr-1 gene was identified as a plasmid-mediated resistance mechanism in human and animal enterobacteriaceae, with a wide geographical distribution and many producer strains resistant to multiple other antibiotics. mcr-1 encodes a membrane-bound enzyme catalysing phosphoethanolamine transfer onto bacterial lipid a. here we present crystal structures revealing the mcr-1 periplasmi ... | 2017 | 28059088 |
| first case report of myopericarditis linked to campylobacter coli enterocolitis. | campylobacter spp. are a common cause of mostly self-limiting enterocolitis. although rare, pericarditis and myopericarditis have been increasingly documented as complications following campylobacteriosis. such cases have occurred predominantly in younger males, and involved a single causative species, namely campylobacter jejuni. we report the first case of myopericarditis following campylobacter coli enterocolitis, with illness occurring in an immunocompetent middle-aged female. | 2017 | 28056838 |
| role of recombinant dna technology to improve life. | in the past century, the recombinant dna technology was just an imagination that desirable characteristics can be improved in the living bodies by controlling the expressions of target genes. however, in recent era, this field has demonstrated unique impacts in bringing advancement in human life. by virtue of this technology, crucial proteins required for health problems and dietary purposes can be produced safely, affordably, and sufficiently. this technology has multidisciplinary applications ... | 2016 | 28053975 |
| rapid molecular approach for simultaneous detection of salmonella spp., shigella spp., and vibrio cholera. | gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including iran. early detection of the gastrointestinal tract pathogens can be extremely important. the aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (pcr) for rapid and simultaneous detection of salmonella spp., shigella spp., and vibrio cholera. | 2016 | 28053842 |
| rehabilitation of a patient with overlap of acute transverse myelitis and bickerstaff's brainstem encephalitis: a case report. | we report on one patient with bickerstaff's brainstem encephalitis (bbe) and associated flaccid weakness. counter to previous studies with bbe which indicate weakness due to guillain-barre syndrome, our patient's presentation of paraplegia following bbe is consistent with concomitant acute transverse myelitis. her findings of bbe largely resolved, although she remained with t6 american spinal injury association (asia) a paraplegia. motor functional impairment measure scores improved from 20 at a ... | 2016 | 28053734 |
| paprbag: a machine learning approach for the detection of novel pathogens from ngs data. | the reliable detection of novel bacterial pathogens from next-generation sequencing data is a key challenge for microbial diagnostics. current computational tools usually rely on sequence similarity and often fail to detect novel species when closely related genomes are unavailable or missing from the reference database. here we present the machine learning based approach paprbag (pathogenicity prediction for bacterial genomes). paprbag overcomes genetic divergence by training on a wide range of ... | 2017 | 28051068 |
| gut microbiome, surgical complications and probiotics. | the trigger for infectious complications in patients following major abdominal operations is classically attributed to endogenous enteral bacterial translocation, due to the critical condition of the gut. today, extensive gut microbiome analysis has enabled us to understand that almost all "evidence-based" surgical or medical intervention (antibiotics, bowel preparation, opioids, deprivation of nutrition), in addition to stress-released hormones, could affect the relative abundance and diversity ... | 2016 | 28042237 |
| gut microbiome, surgical complications and probiotics. | the trigger for infectious complications in patients following major abdominal operations is classically attributed to endogenous enteral bacterial translocation, due to the critical condition of the gut. today, extensive gut microbiome analysis has enabled us to understand that almost all "evidence-based" surgical or medical intervention (antibiotics, bowel preparation, opioids, deprivation of nutrition), in addition to stress-released hormones, could affect the relative abundance and diversity ... | 2016 | 28042237 |
| engineering and dissecting the glycosylation pathway of a streptococcal serine-rich repeat adhesin. | serine-rich repeat glycoproteins (srrps) are conserved in gram-positive bacteria. they are crucial for modulating biofilm formation and bacterial-host interactions. glycosylation of srrps plays a pivotal role in the process; thus understanding the glycosyltransferases involved is key to identifying new therapeutic drug targets. the glycosylation of fap1, an srrp of streptococcus parasanguinis, is mediated by a gene cluster consisting of six genes: gtf1, gtf2, gly, gtf3, dgt1, and galt2 mature fa ... | 2016 | 28039332 |
| characterization and genomic study of the novel bacteriophage hy01 infecting both escherichia coli o157:h7 and shigella flexneri: potential as a biocontrol agent in food. | escherichia coli o157:h7 and shigella flexneri are well-known food-borne pathogens causing severe food poisoning at low infectious doses. bacteriophages have been approved for food applications by the us food and drug administration (fda) and have been suggested as natural food preservatives to control specific food-borne pathogens. to develop a novel natural food preservative against e. coli o157:h7 and s. flexneri, a new bacteriophage needs to be isolated and characterized. | 2016 | 28036349 |
| epidemiological relationships of campylobacter jejuni strains isolated from humans and chickens in south korea. | thirty-nine human isolates of campylobacter jejuni obtained from a national university hospital during 2007-2010 and 38 chicken isolates of c. jejuni were collected from poultry farms during 2009-2010 in south korea were used in this study. campylobacter genomic species and virulence-associated genes were identified by pcr. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were performed to compare their genetic relationships. all isolates were highly resistant to cip ... | 2017 | 28035601 |
| transcriptome analysis of the intracellular facultative pathogen piscirickettsia salmonis: expression of putative groups of genes associated with virulence and iron metabolism. | the intracellular facultative bacteria piscirickettsia salmonis is one of the most important pathogens of the chilean aquaculture. however, there is a lack of information regarding the whole genomic transcriptional response according to different extracellular environments. we used next generation sequencing (ngs) of rna (rna-seq) to study the whole transcriptome of an isolate of p. salmonis (favet-inbiogen) using a cell line culture and a modified cell-free liquid medium, with or without iron s ... | 2016 | 28033422 |
| structural investigation on wlarg from campylobacter jejuni: a sugar aminotransferase. | campylobacter jejuni is a gram-negative bacterium that represents a leading cause of human gastroenteritis worldwide. of particular concern is the link between c. jejuni infections and the subsequent development of guillain-barré syndrome, an acquired autoimmune disorder leading to paralysis. all gram-negative bacteria contain complex glycoconjugates anchored to their outer membranes, but in most strains of c. jejuni, this lipoglycan lacks the o-antigen repeating units. recent mass spectrometry ... | 2017 | 28028852 |
| detection of campylobacter jejuni in lizard faeces from central australia using quantitative pcr. | worldwide, campylobacter is a significant cause of gastrointestinal illness. it is predominately considered a foodborne pathogen, with human exposure via non-food transmission routes generally overlooked. current literature has been exploring environmental reservoirs of campylobacteriosis including potential wildlife reservoirs. given the close proximity between lizards and human habitats in central australia, this study examined the presence of campylobacter jejuni from lizard faeces collected ... | 2016 | 28025556 |
| relaxation of dna supercoiling leads to increased invasion of epithelial cells and protein secretion by campylobacter jejuni. | invasion of intestinal epithelial cells by campylobacter jejuni is a critical step during infection of the intestine by this important human pathogen. in this study we investigated the role played by dna supercoiling in the regulation of invasion of epithelial cells and the mechanism by which this could be mediated. a significant correlation between more relaxed dna supercoiling and an increased ability of c. jejuni strains to penetrate human epithelial cells was demonstrated. directly inducing ... | 2017 | 28019693 |
| concurrency of guillain-barre syndrome and acute transverse myelitis: a case report and review of literature. | guillain-barré syndrome and acute transverse myelitis manifest as demyelinating diseases of the peripheral and central nervous system. concurrency of these two disorders is rarely documented in literature. a 4-year-old girl presenting with cough, fever, and an impaired walking ability was admitted to hospital. she had no previous complaints in her medical history. a physical examination revealed lack of muscle strength of the lower extremities and deep tendon reflexes. mri could not be carried o ... | 2016 | 28018472 |
| the capricious nature of bacterial pathogens: phasevarions and vaccine development. | infectious diseases are a leading cause of morbidity and mortality worldwide, and vaccines are one of the most successful and cost-effective tools for disease prevention. one of the key considerations for rational vaccine development is the selection of appropriate antigens. antigens must induce a protective immune response, and this response should be directed to stably expressed antigens so the target microbe can always be recognized by the immune system. antigens with variable expression, due ... | 2016 | 28018352 |
| antibodies from multiple sclerosis patients preferentially recognize hyperglucosylated adhesin of non-typeable haemophilus influenzae. | in autoimmune diseases, there have been proposals that exogenous "molecular triggers", i.e., specific this should be 'non-self antigens' accompanying infectious agents, might disrupt control of the adaptive immune system resulting in serious pathologies. the etiology of the multiple sclerosis (ms) remains unclear. however, epidemiologic data suggest that exposure to infectious agents may be associated with increased ms risk and progression may be linked to exogenous, bacterially-derived, antigen ... | 2016 | 28008952 |
| a proteomics assay to detect eight cbrn-relevant toxins in food. | a proteomics assay was set up to analyze food substrates for eight toxins of the cbrn (chemical, biological, radiological and nuclear) threat, namely ricin, clostridium perfringens epsilon toxin (etx), staphylococcus aureus enterotoxins (sea, seb and sed), shigatoxins from shigella dysenteriae and entero-hemorragic escherichia coli strains (stx1 and stx2) and campylobacter jejuni cytolethal distending toxin (cdt). the assay developed was based on an antibody-free sample preparation followed by b ... | 2017 | 28008711 |
| host cell binding of the flagellar tip protein of campylobacter jejuni. | flagella are nanofibers that drive bacterial movement. the filaments are generally composed of thousands of tightly packed flagellin subunits with a terminal cap protein, named flid. here, we report that the flid protein of the bacterial pathogen campylobacter jejuni binds to host cells. live-cell imaging and confocal microscopy showed initial contact of the bacteria with epithelial cells via the flagella tip. recombinant flid protein bound to the surface of intestinal epithelial cells in a dose ... | 2016 | 28008697 |
| high resolution crystal structure of the catalytic domain of mcr-1. | the newly identified mobile colistin resistant gene (mcr-1) rapidly spread among different bacterial strains and confers colistin resistance to its host, which has become a global concern. based on sequence alignment, mcr-1 should be a phosphoethanolamine transferase, members of the yhjw/yjdb/yijp superfamily and catalyze the addition of phosphoethanolamine to lipid a, which needs to be validated experimentally. here we report the first high-resolution crystal structure of the c-terminal catalyt ... | 2016 | 28000749 |
| antimicrobial usage and resistance in beef production. | antimicrobials are critical to contemporary high-intensity beef production. many different antimicrobials are approved for beef cattle, and are used judiciously for animal welfare, and controversially, to promote growth and feed efficiency. antimicrobial administration provides a powerful selective pressure that acts on the microbial community, selecting for resistance gene determinants and antimicrobial-resistant bacteria resident in the bovine flora. the bovine microbiota includes many harmles ... | 2016 | 27999667 |
| the cytolethal distending toxin produced by nontyphoidal salmonella serotypes javiana, montevideo, oranienburg, and mississippi induces dna damage in a manner similar to that of serotype typhi. | select nontyphoidal salmonella enterica (nts) serotypes were recently found to encode the salmonella cytolethal distending toxin (s-cdt), an important virulence factor for serotype typhi, the causative agent of typhoid fever. using a pcr-based assay, we determined that among 21 nts serotypes causing the majority of food-borne salmonellosis cases in the united states, genes encoding s-cdt are conserved in isolates representing serotypes javiana, montevideo, and oranienburg but that among serotype ... | 2016 | 27999166 |
| bacteriophage-based tools: recent advances and novel applications. | bacteriophages (phages) are viruses that infect bacterial hosts, and since their discovery over a century ago they have been primarily exploited to control bacterial populations and to serve as tools in molecular biology. in this commentary, we highlight recent diverse advances in the field of phage research, going beyond bacterial control using whole phage, to areas including biocontrol using phage-derived enzybiotics, diagnostics, drug discovery, novel drug delivery systems and bionanotechnolo ... | 2016 | 27990274 |
| tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications. | tannerella forsythia is an anaerobic, gram-negative periodontal pathogen. a unique o-linked oligosaccharide decorates the bacterium's cell surface proteins and was shown to modulate the host immune response. in our study, we investigated the biosynthesis of the nonulosonic acid (nulo) present at the terminal position of this glycan. a bioinformatic analysis of t. forsythia genomes revealed a gene locus for the synthesis of pseudaminic acid (pse) in the type strain atcc 43037 while strains fdc 92 ... | 2017 | 27986835 |
| dissecting the interplay between intestinal microbiota and host immunity in health and disease: lessons learned from germfree and gnotobiotic animal models. | this review elaborates the development of germfree and gnotobiotic animal models and their application in the scientific field to unravel mechanisms underlying host-microbe interactions and distinct diseases. strictly germfree animals are raised in isolators and not colonized by any organism at all. the germfree state is continuously maintained by birth, raising, housing and breeding under strict sterile conditions. however, isolator raised germfree mice are exposed to a stressful environment an ... | 2016 | 27980855 |
| metagenome phylogenetic profiling of microbial community evolution in a tetrachloroethene-contaminated aquifer responding to enhanced reductive dechlorination protocols. | chlorinated solvent contamination of potable water supplies is a serious problem worldwide. biostimulation protocols can successfully remediate chlorinated solvent contamination through enhanced reductive dechlorination pathways, however the process is poorly understood and sometimes stalls creating a more serious problem. whole metagenome techniques have the potential to reveal details of microbial community changes induced by biostimulation. here we compare the metagenome of a tetrachloroethen ... | 2016 | 27980706 |
| health-related aspects of milk proteins. | milk is an important component of a balanced diet and contains numerous valuable constituents. considerable acclaimed health benefits of milk are related to its proteins, not only for their nutritive value but also for their biological properties. scientific evidence suggests that anticarcinogenic activities, antihypertensive properties, immune system modulation, and other metabolic features of milk, are affiliated with its proteins (intact proteins or its derivatives). in this article, the main ... | 2016 | 27980594 |
| feed can be a source of campylobacter jejuni infection in broilers. | 1. the aim was to determine the importance of a contaminated diet as a possible cause of campylobacter jejuni infection in broilers. 2. this study evaluated the viability of c. jejuni in both starter and finisher diets and the interference from other mesophilic bacteria in this viability. 3. starter and finisher samples of broiler diet were deliberately contaminated with 3 or 5 log cfu·g(-1) of c. jejuni (nctc 11351) and then maintained at two different storage temperatures (25°c or 37°c) for 3 ... | 2017 | 27977299 |
| editorial: biofilms from a food microbiology perspective: structures, functions, and control strategies. | 2016 | 27965657 | |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| the minor flagellin of campylobacter jejuni (flab) confers defensive properties against bacteriophage infection. | a screen of bacteriophages infecting a panel of campylobacter jejuni pt14 gene knock-out mutants identified a role for the minor flagellin encoded by the flab gene, in the defense of the host against cp8unalikevirus bacteriophage cp_f1 infection. inactivation of the flab gene resulted in an increase in the susceptibility of pt14 cultures to infection by cp_f1 and an increase in bacteriophage yields. infection of wild type pt14 with cp_f1 produces turbid plaques in bacterial lawns, from which 78% ... | 2016 | 27965643 |
| use of single molecule sequencing for comparative genomics of an environmental and a clinical isolate of clostridium difficile ribotype 078. | how the pathogen clostridium difficile might survive, evolve and be transferred between reservoirs within the natural environment is poorly understood. some ribotypes are found both in clinical and environmental settings. whether these strains are distinct from each another and evolve in the specific environments is not established. the possession of a highly mobile genome has contributed to the genetic diversity and ongoing evolution of c. difficile. interpretations of genetic diversity have be ... | 2016 | 27964731 |
| anti-gq1b-negative miller fisher syndrome with acute areflexic mydriasis and cholinergic supersensitivity. | miller fisher syndrome is a rare variant of guillain-barré syndrome and it is characterised by ophthalmoplegia, ataxia, and areflexia. pupillomotor involvement occurs in approximately half of the patients with the disorder. the authors report a patient with acute areflexic mydriasis, external ophthalmoplegia, areflexia, and ataxia. although the pupils were unreactive to light and near stimuli, administration of 0.1% pilocarpine resulted in marked miosis, suggesting cholinergic supersensitivity. ... | 2011 | 27956933 |
| cytolethal distending toxin from campylobacter jejuni requires the cytoskeleton for toxic activity. | campylobacter jejuni is one of the major causes of infectious diarrhea worldwide. the distending cytolethal toxin (cdt) of campylobacter spp. interferes with normal cell cycle progression. this toxic effect is considered a result of dnase activity that produces chromosomal dna damage. to perform this event, the toxin must be endocytosed and translocated to the nucleus. | 2016 | 27942359 |
| guillain barré syndrome is induced in non-obese diabetic (nod) mice following campylobacter jejuni infection and is exacerbated by antibiotics. | campylobacter jejuni is a leading cause of bacterial gastroenteritis linked to several serious autoimmune sequelae such as the peripheral neuropathies guillain barré syndrome (gbs) and miller fisher syndrome (mfs). we hypothesized that gbs and mfs can result in nod wild type (wt) mice or their congenic interleukin (il)-10 or b7-2 knockouts secondary to c. jejuni infection. mice were gavaged orally with c. jejuni strains hb93-13 and 260.94 from patients with gbs or cf93-6 from a patient with mfs ... | 2017 | 27939129 |
| patient-specific bacteroides genome variants in pouchitis. | a 2-year longitudinal microbiome study of 22 patients who underwent colectomy with an ileal pouch anal anastomosis detected significant increases in distinct populations of bacteroides during 9 of 11 patient visits that coincided with inflammation (pouchitis). oligotyping and metagenomic short-read annotation identified bacteroides populations that occurred in early samples, bloomed during inflammation, and reappeared after antibiotic treatment. targeted cultivation of bacteroides isolates from ... | 2016 | 27935837 |
| the pas domain-containing protein heur regulates heme uptake in campylobacter jejuni. | campylobacter jejuni is a leading cause of bacterially derived gastroenteritis. a previous mutant screen demonstrated that the heme uptake system (chu) is required for full colonization of the chicken gastrointestinal tract. subsequent work identified a pas domain-containing regulator, termed heur, as being required for chicken colonization. here we confirm that both the heme uptake system and heur are required for full chicken gastrointestinal tract colonization, with the heur mutant being part ... | 2016 | 27935836 |
| life without dutpase. | fine-tuned regulation of the cellular nucleotide pools is indispensable for faithful replication of deoxyribonucleic acid (dna). the genetic information is also safeguarded by dna damage recognition and repair processes. uracil is one of the most frequently occurring erroneous bases in dna; it can arise from cytosine deamination or thymine-replacing incorporation. two enzyme activities are primarily involved in keeping dna uracil-free: dutpase (dutp pyrophosphatase) activity that prevent thymine ... | 2016 | 27933035 |
| a novel, molybdenum-containing methionine sulfoxide reductase supports survival of haemophilus influenzae in an in vivo model of infection. | haemophilus influenzae is a host adapted human mucosal pathogen involved in a variety of acute and chronic respiratory tract infections, including chronic obstructive pulmonary disease and asthma, all of which rely on its ability to efficiently establish continuing interactions with the host. here we report the characterization of a novel molybdenum enzyme, torz/mtsz that supports interactions of h. influenzae with host cells during growth in oxygen-limited environments. strains lacking torz/mts ... | 2016 | 27933034 |
| complete genome sequence of bacteriophage ma12, which infects both campylobacter jejuni and salmonella enterica serovar enteritidis. | here, we announce the complete genome sequence of salmonella enterica serovar enteritidis (s enteritidis) bacteriophage ma12, a 41-kb chromosome. the strain can infect both campylobacter jejuni (c. jejuni) and s enteritidis and can be used in phage therapy experiments with poultry and poultry meat. | 2016 | 27932636 |
| htra-mediated e-cadherin cleavage is limited to degp and degq homologs expressed by gram-negative pathogens. | the serine proteases htra/degp secreted by the human gastrointestinal pathogens helicobacter pylori (h. pylori) and campylobacter jejuni (c. jejuni) cleave the mammalian cell adhesion protein e-cadherin to open intercellular adhesions. a wide range of bacteria also expresses the htra/degp homologs degq and/or degs, which significantly differ in structure and function. | 2016 | 27931258 |
| exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes. | fatigue, mood disturbances, under performance and gastrointestinal distress are common among athletes during training and competition. the psychosocial and physical demands during intense exercise can initiate a stress response activating the sympathetic-adrenomedullary and hypothalamus-pituitary-adrenal (hpa) axes, resulting in the release of stress and catabolic hormones, inflammatory cytokines and microbial molecules. the gut is home to trillions of microorganisms that have fundamental roles ... | 2016 | 27924137 |
| characterization in helicobacter pylori of a nickel transporter essential for colonization that was acquired during evolution by gastric helicobacter species. | metal acquisition is crucial for all cells and for the virulence of many bacterial pathogens. in particular, nickel is a virulence determinant for the human gastric pathogen helicobacter pylori as it is the cofactor of two enzymes essential for in vivo colonization, urease and a [nife] hydrogenase. to import nickel despite its scarcity in the human body, h. pylori requires efficient uptake mechanisms that are only partially defined. indeed, alternative ways of nickel entry were predicted to exis ... | 2016 | 27923069 |
| age-related differences in the luminal and mucosa-associated gut microbiome of broiler chickens and shifts associated with campylobacter jejuni infection. | despite the importance of gut microbiota for broiler performance and health little is known about the composition of this ecosystem, its development and response towards bacterial infections. therefore, the current study was conducted to address the composition and structure of the microbial community in broiler chickens in a longitudinal study from day 1 to day 28 of age in the gut content and on the mucosa. additionally, the consequences of a campylobacter (c.) jejuni infection on the microbia ... | 2016 | 27921008 |
| a current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens. | gastroenteritis is caused by a wide range of viral, bacterial and parasitic pathogens and causes millions of deaths worldwide each year, particularly in infant populations in developing countries. traditional microbiological culture and immunological based tests are time consuming, laborious and often lack diagnostic specificity and sensitivity. as a result patients can receive suboptimal and/or inappropriate antimicrobial treatment. in recent years, rapid nucleic acid diagnostics (nad) technolo ... | 2014 | 27920992 |
| glucose metabolism via the entner-doudoroff pathway in campylobacter: a rare trait that enhances survival and promotes biofilm formation in some isolates. | isolates of the zoonotic pathogen campylobacter are generally considered to be unable to metabolize glucose due to lack of key glycolytic enzymes. however, the entner-doudoroff (ed) pathway has been identified in campylobacter jejuni subsp. doylei and a few c. coli isolates. a systematic search for ed pathway genes in a wide range of campylobacter isolates and in the c. jejuni/coli pubmlst database revealed that 1.7% of >6,000 genomes encoded a complete ed pathway, including both c. jejuni and c ... | 2016 | 27920773 |
| one-step pcr detection of salmonella pullorum/gallinarum using a novel target: the flagellar biosynthesis gene flhb. | salmonella enterica serovar pullorum/gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. traditional salmonella serotyping is an expensive and time-consuming process. in this study, we developed a rapid one-step polymerase chain reaction (pcr) method to identify s. pullorum/gallinarum. the pcr-based assay focuses on flhb, which shows a deficient region only in s. pullorum/gallinarum, compared with that of other serovars. the specificity and se ... | 2016 | 27920764 |
| advances and challenges in viability detection of foodborne pathogens. | foodborne outbreaks are a serious public health and food safety concern worldwide. there is a great demand for rapid, sensitive, specific, and accurate methods to detect microbial pathogens in foods. conventional methods based on cultivation of pathogens have been the gold standard protocols; however, they take up to a week to complete. molecular assays such as polymerase chain reaction (pcr), sequencing, microarray technologies have been widely used in detection of foodborne pathogens. among mo ... | 2016 | 27920757 |
| development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of shigella spp. | shigella spp., the etiological agent of shigellosis or "bacillary dysentery," are responsible for considerable morbidity and mortality in excess of a million deaths globally per year. although pcr-based techniques (such as pcr-based dipstick biosensors) have been used for the molecular diagnosis of infectious disease, these assays were restricted due to the need for a sophisticated thermal cycling apparatus to denature target templates. to facilitate simple and rapid detection of target pathogen ... | 2016 | 27917160 |
| rapid, sensitive detection of bartonella quintana by loop-mediated isothermal amplification of the groel gene. | trench fever, caused by bartonella quintana, is recognized as a re-emerging and neglected disease. rapid and sensitive detection approaches are urgently required to monitor and help control b. quintana infections. here, loop-mediated isothermal amplification (lamp), which amplifies target dna at a fixed temperature with high sensitivity, specificity and rapidity, was employed to detect b. quintana. thirty-six strains, including 10 b. quintana, 13 other bartonella spp., and 13 other common pathog ... | 2016 | 27916953 |
| impact of waning acquired immunity and asymptomatic infections on case-control studies for enteric pathogens. | case-control studies of outbreaks and of sporadic cases of infectious diseases may provide a biased estimate of the infection rate ratio, due to selecting controls that are not at risk of disease. we use a dynamic mathematical model to explore biases introduced in results drawn from case-control studies of enteric pathogens by waning and boosting of immunity, and by asymptomatic infections, using campylobacter jejuni as an example. individuals in the population are either susceptible (at risk of ... | 2016 | 27915211 |
| genomic insights from whole genome sequencing of four clonal outbreak campylobacter jejuni assessed within the global c. jejuni population. | whole genome sequencing (wgs) is useful for determining clusters of human cases, investigating outbreaks, and defining the population genetics of bacteria. it also provides information about other aspects of bacterial biology, including classical typing results, virulence, and adaptive strategies of the organism. cell culture invasion and protein expression patterns of four related multilocus sequence type 21 (st21) c. jejuni isolates from a significant canadian water-borne outbreak were previou ... | 2016 | 27912729 |
| genomic variations leading to alterations in cell morphology of campylobacter spp. | campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. however, it can also adopt straight rod, elongated helical and coccoid forms. studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. here, we aimed to determine the genetic factors involved in generating the helical shape of campylobacter. a c. jejuni transposon (tn) mutant library was screened for non ... | 2016 | 27910897 |
| draft genome sequences of campylobacter jejuni strains that cause abortion in livestock. | campylobacter jejuni is an intestinal bacterium that can cause abortion in livestock. this publication announces the public release of 15 campylobacter jejuni genome sequences from isolates linked to abortion in livestock. these isolates are part of the 100k pathogen genome project and are from clinical cases at the university of california (uc) davis. | 2016 | 27908990 |
| complementation of an aglb mutant of methanococcus maripaludis with heterologous oligosaccharyltransferases. | the oligosaccharyltransferase is the signature enzyme for n-linked glycosylation in all domains of life. in archaea, this enzyme termed aglb, is responsible for transferring lipid carrier-linked glycans to select asparagine residues in a variety of target proteins including archaellins, s-layer proteins and pilins. this study investigated the ability of a variety of aglbs to compensate for the oligosaccharyltransferase activity in methanococcus maripaludis deleted for aglb, using archaellin flab ... | 2016 | 27907170 |
| a dual filtration-based multiplex pcr method for simultaneous detection of viable escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on fresh-cut cantaloupe. | fresh-cut cantaloupe is particularly susceptible to contamination with pathogenic bacteria, such as escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus. therefore, development of rapid, yet accurate detection techniques is necessary to ensure food safety. in this study, a multiplex pcr system and propidium monoazide (pma) concentration were optimized to detect all viable pathogens in a single tube. a dual filtration system utilized a filtration membrane with different por ... | 2016 | 27906992 |
| epidemiological and serological investigation of a waterborne campylobacter jejuni outbreak in a danish town. | following an unusually heavy rainfall in june 2009, a community-wide outbreak of campylobacter gastroenteritis occurred in a small danish town. the outbreak investigation consisted of (1) a cohort study using an e-questionnaire of disease determinants, (2) microbiological study of stool samples, (3) serological study of blood samples from cases and asymptomatic members of case households, and (4) environmental analyses of the water distribution system. the questionnaire study identified 163 case ... | 2017 | 27903324 |
| the two-component system arlrs and alterations in metabolism enable staphylococcus aureus to resist calprotectin-induced manganese starvation. | during infection the host imposes manganese and zinc starvation on invading pathogens. despite this, staphylococcus aureus and other successful pathogens remain capable of causing devastating disease. however, how these invaders adapt to host-imposed metal starvation and overcome nutritional immunity remains unknown. we report that arlrs, a global staphylococcal virulence regulator, enhances the ability of s. aureus to grow in the presence of the manganese-and zinc-binding innate immune effector ... | 2016 | 27902777 |
| campylobacter jejuni strains coresistant to tetracycline and ciprofloxacin in patients with gastroenteritis in croatia(). | antibiotic-resistant campylobacter jejuni strains are rapidly emerging worldwide. here, we aimed to determine the antibiotic-resistance patterns and genetic structure of c. jejuni from stool samples of symptomatic patients in dalmatia, the largest croatian county. | 2017 | 27900889 |
| bibliometric analysis of publications on campylobacter: (2000-2015). | campylobacter species are widespread zoonotic pathogens. campylobacter jejuni causes a form of gastroenteritis called campylobacteriosis. campylobacter drug resistance is considered a serious threat. in order to better understand national and international research output on campylobacter, we conducted this bibliometric overview of publications on campylobacter. this study can be used to assess extent of interaction and response of researchers, food regulators, and health policy makers to global ... | 2016 | 27899145 |
| environmental free-living amoebae isolated from soil in khon kaen, thailand, antagonize burkholderia pseudomallei. | presence of burkholderia pseudomallei in soil and water is correlated with endemicity of melioidosis in southeast asia and northern australia. several biological and physico-chemical factors have been shown to influence persistence of b. pseudomallei in the environment of endemic areas. this study was the first to evaluate the interaction of b. pseudomallei with soil amoebae isolated from b. pseudomallei-positive soil site in khon kaen, thailand. four species of amoebae, paravahlkampfia ustiana, ... | 2016 | 27898739 |
| dcite: measuring necessary cladistic information can help you reduce polytomy artefacts in trees. | biologists regularly create phylogenetic trees to better understand the evolutionary origins of their species of interest, and often use genomes as their data source. however, as more and more incomplete genomes are published, in many cases it may not be possible to compute genome-based phylogenetic trees due to large gaps in the assembled sequences. in addition, comparison of complete genomes may not even be desirable due to the presence of horizontally acquired and homologous genes. a decision ... | 2016 | 27898695 |
| pneumococcal polysaccharide vaccination elicits igg anti-a/b blood group antibodies in healthy individuals and patients with type i diabetes mellitus. | blood group antibodies are natural antibodies that develop early in life in response to cross-reactive environmental antigens in the absence of antigen encounter. even later in life structural similarities in saccharide composition between environmental antigens such as bacterial polysaccharides and blood group a/b antigens could lead to changes in serum levels, igm/igg isotype, and affinity maturation of blood group anti-a/b antibodies. we addressed the question whether immunization with pneumo ... | 2016 | 27895641 |
| azithromycin vs. placebo for the clinical outcome in campylobacter concisus diarrhoea in adults: a randomized, double-blinded, placebo-controlled clinical trial. | campylobacter concisus has been associated with prolonged mild diarrhoea, but investigations regarding the efficacy of antimicrobial treatment have not been reported previously. we initiated a phase 3, single-centre, randomized, double-blinded, placebo-controlled study comparing the efficacy of 500 mg once-daily dose of azithromycin with a 500 mg once-daily dose of placebo for three days, for the treatment of c. concisus diarrhoea in adult patients with a follow-up period of ten days. if symptom ... | 2016 | 27893820 |
| effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice. | more than 50 years after the discovery of antibiotics, bacterial infections have decreased substantially; however, antibiotics also may have negative effects such as increasing susceptibility to pathogens. an intact microbiome is an important line of defense against pathogens. we sought to determine the effect of orally administered antibiotics both on susceptibility to pathogens and on impact to the microbiome. we studied campylobacter jejuni, one of the most common causes of human diarrhea, an ... | 2016 | 27891184 |
| acute disseminated encephalomyelitis following campylobacter jejuni gastroenteritis: case report and review of the literature. | we describe a case of a 25-year-old male with a diagnosis of acute disseminated encephalomyelitis (adem) following infection with campylobacter jejuni, which is implicated in various human pathologies regarding the central nervous system (cns) with acute course like guillain-barré syndrome (gbs), miller-fisher syndrome (mfs), bickerstaff's brainstem encephalitis (bee), acute transverse myelitis (atm) as well as adem. these conditions are caused by cross-reactivity between campylobacter's epitope ... | 2017 | 27888275 |
| generation and screening of an insertion sequencing-compatible mutant library of campylobacter jejuni. | the advent of next-generation sequencing technology has enabled experimental approaches to characterize large, complex populations of dna molecules with high resolution. included among these are methods to assess populations of transposon insertion libraries for the fitness cost of any particular mutant allele after applying selection to a population. these approaches have proven invaluable for identifying genetic factors that influence survival of bacterial pathogens within different environmen ... | 2017 | 27885613 |
| analyzing prokaryotic rna-seq data: a case study identifying holo-fur regulated genes in campylobacter jejuni. | in recent years, rna-seq has become an important method in the process of measuring gene expression in various cells and organisms. this chapter will detail all the bioinformatic steps that should be undertaken to determine differentially expressed genes from a typical rna-seq experiment. each step will be clearly explained in "non-bioinformatic" terminology so that readers embarking on rna-seq analysis will be able to understand the rationale and reasoning behind each step. moreover, the exact ... | 2017 | 27885612 |
| methods for proteome analysis of campylobacter jejuni using 2-d electrophoresis. | this chapter describes protocols used for two-dimensional electrophoretic analysis of the proteome or subproteome of campylobacter jejuni, a major human food-borne pathogen. the following protocols, adapted to campylobacter strains, include all the steps from cultivation to gel-support protein separation. | 2017 | 27885611 |
| characterizing glycoproteins by mass spectrometry in campylobacter jejuni. | the glycosylation systems of campylobacter jejuni (c. jejuni) are considered archetypal examples of both n- and o-linked glycosylations in the field of bacterial glycosylation. the discovery and characterization of these systems both have revealed important biological insight into c. jejuni and have led to the refinement and enhancement of methodologies to characterize bacterial glycosylation. in general, mass spectrometry-based characterization has become the preferred methodology for the study ... | 2017 | 27885610 |
| methods for genome-wide methylome profiling of campylobacter jejuni. | methylation has a profound role in the regulation of numerous biological processes in bacteria including virulence. the study of methylation in bacteria has greatly advanced thanks to next-generation sequencing technologies. these technologies have expedited the process of uncovering unique features of many bacterial methylomes such as characterizing previously uncharacterized methyltransferases, cataloging genome-wide dna methylations in bacteria, identifying the frequency of methylation at par ... | 2017 | 27885609 |
| metabolomic analysis of campylobacter jejuni by direct-injection electrospray ionization mass spectrometry. | direct-injection mass spectrometry (dims) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. given our generally poor understanding of campylobacter metabolism, the high-throughput and relatively simple sample preparation of dims has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. here we describe a metabolomic fingerprinting app ... | 2017 | 27885608 |
| mouse models for campylobacter jejuni colonization and infection. | relevant animal models for campylobacter jejuni infection have been difficult to establish due to c. jejuni's inability to cause disease in many common animal research models. fortunately, recent work has proven successful in developing several new and relevant mouse models of c. jejuni infection, including the sigirr-deficient mouse strain that develops acute enterocolitis in response to c. jejuni. here we describe how to properly infect mice with c. jejuni, as well as a number of accompanying ... | 2017 | 27885607 |
| using galleria mellonella as an infection model for campylobacter jejuni pathogenesis. | nonmammalian model systems of infection have been employed recently to study bacterial virulence. for instance, galleria mellonella (the greater wax moth) has been shown to be susceptible to infection by many bacterial pathogens including the enteric pathogen campylobacter jejuni. in contrast to the traditional animal models for c. jejuni such as the chick colonization model and ferret diarrheal model, the galleria mellonella infection model has the advantages of lower cost, ease of use and no a ... | 2017 | 27885606 |
| characterization of ligand-receptor interactions: chemotaxis, biofilm, cell culture assays, and animal model methodologies. | chemotactic motility is an essential virulence factor for the pathogenesis of campylobacter spp. infection. in chapter 6 , we described technologies that enable initial screening and identification of ligands able to interact with chemoreceptor sensory domains. these include amino acid and glycan arrays, nmr, and spr that are utilized to identify potential ligands interacting with campylobacter jejuni. here we describe techniques that enable the characterization and evaluation of ligand-receptor ... | 2017 | 27885605 |
| assays to study the interaction of campylobacter jejuni with the mucosal surface. | mucosal colonization and overcoming the mucosal barrier are essential steps in the establishment of infection by campylobacter jejuni. the interaction between c. jejuni and host cells, including binding and invasion, is thought to be the key virulence factor important for pathogenesis of c. jejuni infections in animals or humans. the intestinal mucosal barrier is composed of a polarized epithelium covered by a thick adherent mucus gel layer. there is a requirement for cell culture assays of infe ... | 2017 | 27885604 |
| methods to study campylobacter jejuni adherence to and invasion of host epithelial cells. | measuring bacterial adherence and invasion of cells in vitro has enabled researchers to dissect the interactions of campylobacter jejuni with eukaryotic cells. numerous c. jejuni virulence determinants and host cell factors that contribute to the process of adherence, invasion, and immune modulation have been identified utilizing in vitro adherence and invasion assays. in this chapter, we describe the evaluation of c. jejuni adherence to and invasion of hela cells using the gentamicin-protection ... | 2017 | 27885603 |
| methods to assess the direct interaction of c. jejuni with mucins. | studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. here we describe a far western blotting approach to show how c. jejuni proteins separated by sds page and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. in addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a h ... | 2017 | 27885602 |
| methods for initial characterization of campylobacter jejuni bacteriophages. | here we describe an initial characterization of campylobacter jejuni bacteriophages by host range analysis, genome size determination by pulsed-field gel electrophoresis, and receptor-type identification by screening mutants for phage sensitivity. | 2017 | 27885601 |
| method for the successful crystallization of the ferric uptake regulator from campylobacter jejuni. | the ferric uptake regulator (fur) is a transcription factor (tf) regulating the expression of several genes to control iron levels in prokaryotes. members of this family of tfs share a common structural scaffold that typically comprises two regions that include a dna binding and dimerization domains. while this structural organization is conserved, fur proteins employ different mechanisms to bind divergent dna binding elements and regulate gene expression in the absence or presence of regulatory ... | 2017 | 27885600 |
| characterization of high affinity iron acquisition systems in campylobacter jejuni. | iron acquisition systems are critical for bacterial pathogenesis and thus have been proposed as attractive targets for iron-dependent pathogen control. of these systems, high-affinity iron acquisition mediated by siderophore, a small iron chelator, is the most efficient iron-scavenging mechanism in gram-negative bacteria. campylobacter does not produce any siderophores but has the ability to utilize exogenous siderophores. in particular, the enterobactin (ent)-mediated iron scavenging is tightly ... | 2017 | 27885599 |
| method of peptide nucleic acid (pna)-mediated antisense inhibition of gene expression in campylobacter jejuni. | peptide nucleic acid (pna) is an oligonucleotide mimic that recognizes and binds to nucleic acids. the strong binding affinity of pna to mrna coupled with its high sequence specificity enable antisense pna to selectively inhibit (i.e., knockdown) the protein synthesis of a target gene. this novel technology provides a powerful tool for campylobacter studies because molecular techniques have been relatively less well-developed for this bacterium as compared to other pathogens, such as escherichia ... | 2017 | 27885597 |
| methods to study antimicrobial resistance in campylobacter jejuni. | campylobacter jejuni is a leading bacterial cause of foodborne gastroenteritis worldwide and is increasingly resistant to clinically important antibiotics. detection of antibiotic resistance in c. jejuni can be performed with both phenotypic and genotypic methods. in this chapter, we describe the most commonly used molecular biology methods for detection of resistance to clinically important antibiotics. these methods can be employed in both clinical and research settings to facilitate clinical ... | 2017 | 27885596 |
| methods for isolation, purification, and propagation of bacteriophages of campylobacter jejuni. | here, we describe the methods for isolation, purification, and propagation of campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. the overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on c. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stock ... | 2017 | 27885595 |
| campylobacter jejuni isolation/enumeration from environmental samples. | currently, there is no universally accepted standard media or method for the recovery of campylobacter species. this is likely due to the ubiquity of the organism in nature, the complex sample matrices from which the organism is often recovered, as well as the fragile/viable-but nonculturable state the organism assumes in response to stress. the use of a sterile filter placed upon a nonselective brucella agar blood plate (bab), followed by incubation at 37 °c in a hydrogen-containing atmosphere ... | 2017 | 27885593 |
| in-package inactivation of pathogenic and spoilage bacteria associated with poultry using dielectric barrier discharge-cold plasma treatments. | the goal of this study was to test the efficacy of in-package dielectric barrier discharge-cold plasma (dbd-cp) treatment to inactivate poultry-associated spoilage (pseudomonas fluorescens) and pathogenic (salmonella enterica typhimurium, campylobacter jejuni) bacteria. liquid cultures of the bacterial isolates were sealed within packages containing ambient air (trial 1) or modified air (65% o2:30% co2:5% n2; trial 2). the packages were subjected to treatment times ranging from 30 to 180 s, and ... | 2017 | 27885385 |
| genome-wide association of functional traits linked with campylobacter jejuni survival from farm to fork. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, primarily associated with the consumption of contaminated poultry. c. jejuni lineages vary in host range and prevalence in human infection, suggesting differences in survival throughout the poultry processing chain. from 7343 mlst-characterised isolates, we sequenced 600 c. jejuni and c. coli isolates from various stages of poultry processing and clinical cases. a genome-wide association study (gwas) in c. jejuni st-21 ... | 2017 | 27883255 |
| beyond antimicrobial resistance: evidence for a distinct role of the acrd efflux pump in salmonella biology. | for over 20 years, bacterial multidrug resistance (mdr) efflux pumps have been studied because of their impact on resistance to antimicrobials. however, critical questions remain, including why produce efflux pumps under non-antimicrobial treatment conditions, and why have multiple pumps if their only purpose is antimicrobial efflux? salmonella spp. possess five efflux pump families, including the resistance-nodulation-division (rnd) efflux pumps. notably, the rnd efflux pump acrd has a unique s ... | 2016 | 27879336 |
| catabolite and oxygen regulation of enterohemorrhagic escherichia coli virulence. | the biogeography of the gut is diverse in its longitudinal axis, as well as within specific microenvironments. differential oxygenation and nutrient composition drive the membership of microbial communities in these habitats. moreover, enteric pathogens can orchestrate further modifications to gain a competitive advantage toward host colonization. these pathogens are versatile and adept when exploiting the human colon. they expertly navigate complex environmental cues and interkingdom signaling ... | 2016 | 27879335 |
| altered intestinal microbiota-host mitochondria crosstalk in new onset crohn's disease. | intestinal microbial dysbiosis is associated with crohn's disease (cd). however, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. in this report, we use systems-level approaches to study the interactions between the gut microbiota and host in new-onset paediatric patients to evaluate causality and mechanisms of disease. we report an altered host proteome in cd patients indicative of impaired mitochondrial functions. in particular, mitocho ... | 2016 | 27876802 |
| array biosensor for toxin detection: continued advances. | the following review focuses on progress made in the last five years with the nrl array biosensor, a portable instrument for rapid and simultaneous detection of multiple targets. since 2003, the array biosensor has been automated and miniaturized for operation at the point-of-use. the array biosensor has also been used to demonstrate (1) quantitative immunoassays against an expanded number of toxins and toxin indicators in food and clinical fluids, and (2) the efficacy of semi-selective molecule ... | 2008 | 27873991 |
| isolation of a point mutation associated with altered expression of the cmeabc efflux pump in a multidrug-resistant campylobacter jejuni population of poultry origin. | the objective of this study was to investigate the antibiotic resistance phenotype of campylobacter jejuni isolates from a poultry flock of broiler production in spain. isolates were characterised by rflp-pcr of the flaa gene and multilocus sequence typing. minimum inhibitory concentrations of quinolones, aminoglycosides, β-lactams, tetracyclines, phenicols, macrolides and lincosamides were determined by etest. determinants of resistance and the regulatory region of the cmeabc operon were invest ... | 2015 | 27873659 |
| zika virus: a review from the virus basics to proposed management strategies. | this review aims to summarize the body of knowledge available on zika virus to date. a comprehensive review of the scientific literature on zika virus was performed with the aim to stress relevant aspects for healthcare professionals in the non-endemic areas. for several years, the zika virus infection was considered an extremely rare exotic disease with poor clinical relevance. however, zika virus has recently gained the attention of the scientific community and public opinion since the virus s ... | 2016 | 27872736 |
| prevalence and antibiotic resistance profile of thermophilic campylobacter spp. of slaughtered cattle and sheep in shiraz, iran | although poultry meat is considered as the main source for human campylobacter infections, there is limited information about non-poultry sources. the present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilic campylobacter spp. in fecal samples of the cattle and sheep in shiraz, iran. a total of 302 fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from shiraz slaughterhouse. the animals were clinically healthy before bein ... | 2016 | 27872721 |
| epidemiology and rapd-pcr typing of thermophilic campylobacters from children under five years and chickens in morogoro municipality, tanzania. | campylobacter species are gram negative and flagellated bacteria under the genus campylobacter, family campylobacteriaceae. these pathogens cause zoonotic infections among human and animal populations. this study was undertaken between december 2006 and may 2007 to determine prevalence, risk factors and genetic diversity of thermophilic campylobacter isolates from children less than 5 years and chickens in morogoro municipality, tanzania. | 2016 | 27871251 |
| lipooligosaccharide locus classes and putative virulence genes among chicken and human campylobacter jejuni isolates. | campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, guillain barré- and miller fischer syndromes. such sequelae have been linked to c. jejuni harboring sialic acid structures in their lipooligosaccharide (los) layer of the cell wall. poultry is an important source of human campylobacter infections but little is known about the prevalence of sialylated c. jejuni isolates and the extent of transmission of such ... | 2016 | 27871232 |