Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| parainfluenza virus type 3 in pigs. | 1986 | 3024379 | |
| the effect of parainfluenza virus type 3 and pasteurella haemolytica on oxygen-dependent and oxygen-independent bactericidal mechanisms of ovine pulmonary phagocytic cells. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica, either alone or 4 or 6 days after the inoculation of parainfluenza virus type 3 (pi3). other groups were inoculated with pi3 followed by veal infusion broth, or with uninfected cell culture fluid followed by veal infusion broth (controls). all lambs were killed 24 h after the second inoculation. pulmonary phagocytic cells were recovered by lavage and separated into alveo ... | 1986 | 3018994 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| conserved structures among the nucleocapsid proteins of the paramyxoviridae: complete nucleotide sequence of human parainfluenza virus type 3 np mrna. | the nucleotide sequence of the mrna coding for the nucleocapsid protein (np) of the paramyxovirus, human parainfluenza virus type 3 (piv-3), has been determined. the np mrna was found to contain 1642 bases, excluding poly(a), and encode a protein of 515 amino acids, with a molecular weight of 57,823. amino acid residues 1 through 420 of piv-3 np protein showed extensive sequence homology with the corresponding amino acids of sendai virus nucleocapsid protein. there was virtually no homology betw ... | 1986 | 3012866 |
| 3'-terminal sequence of human parainfluenza virus 3 genomic rna. | 1986 | 3012476 | |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| [structural organization and proteins of the human type-3 para-influenza virus]. | the structure of human parainfluenza type 3 virus was studied by electron microscopy and virion fractionation by treatment with a detergent and high ionic strength. the protein spectrum of the virus was studied. the presence of 6 structural proteins was revealed of which two, hn and f, are glycoproteins. intracellular cleavage of f0 protein into f1+2 proteins was demonstrated in a pulse-chase experiment. a tighter binding of hn protein than of f protein with the virus lipoprotein membrane was ob ... | 1986 | 3008440 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| parainfluenza virus bronchiolitis. epidemiology and pathogenesis. | an investigation of the epidemiology and pathogenesis of bronchiolitis due to parainfluenza virus (pv) was carried out. bronchiolitis due to pv occurred most commonly in non-caucasian males. breast-fed infants exhibited a reduced risk of developing bronchiolitis. once an episode of pv bronchiolitis occurred, both exposure to cigarette smoke and bottle feeding were associated with an increased frequency of recurrent wheezing, and subsequent infection with respiratory virus almost uniformly result ... | 1986 | 3002169 |
| epizootiological survey of parainfluenza-3, reovirus-3, respiratory syncytial and infectious bovine rhinotracheitis viral antibodies in sheep and goat flocks in quebec. | a serological survey was conducted in an attempt to detect antibodies against bovine respiratory viruses in sheep and goats from seven geographical areas of quebec. sera from 10% of the animals in 182 sheep flocks and 40 goat flocks were collected and specific antibodies against parainfluenza-3, reovirus type 3, respiratory syncytial and infectious bovine rhinotracheitis viruses were detected by hemagglutination-inhibition tests for the former viruses and complement fixation and seroneutralizati ... | 1985 | 3000551 |
| microwave sterilization of nitrous oxide nasal hoods contaminated with virus. | although there exists a desire to eliminate the possibility of cross-infection from microbial contaminated nitrous oxide nasal hoods, effective and practical methods of sterilization in a dental office are unsatisfactory. microwaves have been used to sterilize certain contaminated dental instruments without damage. in this study nasal hoods contaminated with rhinovirus, parainfluenza virus, adenovirus, and herpes simplex virus were sterilized in a modified microwave oven. ninety-five percent of ... | 1985 | 2999676 |
| studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mrnas isolated from infected cells. | the structural proteins of human parainfluenza virus 3, a member of the paramyxovirus family, were characterized by sds-polyacrylamide gel electrophoresis of radiolabeled virus. the purified virion contains at least eight structural proteins, with estimated molecular weights of 251k, 90k, 71k, 68k, 65k, 51k, 35k, and 21k, respectively. three of the polypeptides (71k, 65k, and 51k) were identified as glycoproteins based on their incorporation of [3h]glucosamine. disruption of the virus by triton ... | 1985 | 2998013 |
| comparison of interferon production in cattle after intranasal infection with parainfluenza-3 live vaccine and avirulent ibr/ipv-herpesvirus. | 1985 | 2996264 | |
| synthesis and biological activity of 5-thiobredinin and certain related 5-substituted imidazole-4-carboxamide ribonucleosides. | a number of 5-substituted imidazole-4-carboxamide ribonucleosides were prepared and tested for their biological activity. treatment of 5-chloro-1-beta-d-ribofuranosylimidazole-4-carboxamide (2) with methanethiol provided 5-(methylthio)-1-beta-d-ribofuranosylimidazole-4-carboxamide (3a). similar treatment of 2 with ethanethiol or benzenemethanethiol gave the corresponding 5-ethylthio and 5-benzylthio derivatives 3b and 3c. oxidation of 3a and 3b with m-chloroperoxybenzoic acid furnished the corre ... | 1985 | 2993613 |
| in vivo and in vitro studies on the use of the guinea pig as a model for virus-provoked airway hyperreactivity. | the parainfluenza 3 (p-3)-infected guinea pig was examined as a model for virus-provoked airway hyperreactivity by measuring changes in airway overflow pressure in response to intravenously (iv) administered histamine. in vitro responses of lung parenchymal strips to several contractile substances were also measured. all studies were conducted 4 days after nasal insufflation with p-3 or p-3 growth medium (control). increases in airway overflow pressure caused by histamine were enhanced by p-3 in ... | 1985 | 2992331 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides. | the intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35s]methionine-labeled cell extracts under reducing conditions. all of the virion structural proteins were detected in cell extracts, including: l, 180,000 molecular weight (180k); p, 83k; hn, 69k; np, 66k; f0, 60k; f1, 51k; and m, 38k. p and np were phosphorylated. hn and f were glycosylated. the kinetics of intracellular viral protein synthesis did not d ... | 1985 | 2987519 |
| properties of temperature-sensitive mutants of parainfluenza virus type 3 selected during the course of persistent infection. | we investigated properties of the ts mutants that were selected during the course of persistent infection of vero cells by parainfluenza virus type 3. the mutants demonstrated leakiness when infecting cells at high moi and interfered with the growth of wild type virus, apparently by inhibiting a step prior to rna synthesis. | 1985 | 2986583 |
| a serologic epidemiological study of parainfluenza 1, 2, and 3 viruses in beef cattle and calves in illinois. | beef cattle and calves in four illinois populations were studied. serum was tested by the hemagglutination-inhibition (hi) test for antibodies to parainfluenza viruses (piv) 1, 2, and 3. in all but group 3 a low level of antibody to piv-1 and 2 was demonstrated. the highest prevalence and incidence of hi-antibodies was found for piv-3, followed by piv-1. there may have been a cross-reaction between piv-1 and -3 in group 1 calves. the low level of hi antibodies to piv-1 and 2 may have resulted fr ... | 1985 | 2986256 |
| contribution to laboratory diagnosis of mumps and parainfluenza. | specific igm and igg antibodies to mumps virus (mv) were detected in sera of mumps-patients by elisa in agreement with the results obtained by indirect immunofluorescence (if). of given sera 37.5% contained igm reacting in indirect elisa also with the antigens of parainfluenza virus (piv) t3. in all patients with respiratory illness over 2 years of age, the significant increase of antibodies to piv in haemagglutination inhibition (hi) test was in good correlation with serum igm and igg antibody ... | 1988 | 2906222 |
| complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses. | the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ... | 1986 | 2878059 |
| [pathogenicity of parainfluenza virus type 3 in mice]. | 1988 | 2855381 | |
| parainfluenza 3 infections. | 1988 | 2854823 | |
| bovine parainfluenza-3 virus proteins recognized by antibodies of aerosol-exposed calves. | bovine parainfluenza-3 (bpi-3) virus proteins were radiolabeled with [35s]methionine. control serum containing antibodies to bpi-3 virus obtained from calves that had been immunized by aerosol exposure and by im inoculation and sera of cattle that were exposed to bpi-3 virus by aerosol only were used to immunoprecipitate bpi-3 virus proteins. the immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. viral proteins with estimated molecular weights of 72,00 ... | 1988 | 2854712 |
| antibodies to parainfluenza virus type 3 in minnesota swine. | 1988 | 2849496 | |
| levamisole-induced attenuation of alveolar macrophage dysfunction in respiratory virus-infected calves. | a luminol-amplified chemiluminescence (cl) assay was used to evaluate the effect of levamisole on the metabolic activity of pulmonary alveolar macrophages (pam) from parainfluenza-3 (pi-3) and infectious bovine rhinotracheitis (ibr) virus-infected calves. using selective beta-adrenergic agonists and antagonists, beta 1-adrenoceptors were shown to inhibit the pam reactive oxygen species-dependent cl response. beta 2 adrenoceptors were apparently not important in the regulation of cl in pam of con ... | 1988 | 2844684 |
| current approaches to the development of vaccines effective against parainfluenza viruses. | infection of infants and children with parainfluenza viruses can lead to serious lower respiratory tract disease. in order to review new information on the genetic organization, epidemiology, and immunobiology of these viruses and of respiratory syncytial virus, who, in conjunction with the national institute of allergy and infectious diseases, held a workshop in bethesda, md, usa, 4-5 may 1987. new data on the immunobiology and epidemiology of human parainfluenza 3 virus were presented that sho ... | 1988 | 2844427 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| protective effects of monoclonal antibodies against parainfluenza virus type 3-induced brain infection in hamsters. | a collection of monoclonal antibodies (mabs) against the haemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins of parainfluenza virus type 3 (piv3) was used for passive immunization of intracerebrally infected newborn hamsters. a significant degree of protection was obtained with mabs against both the hn and f viral proteins, but no individual antibody conferred complete protection against disease. mabs against different epitopes provided different degrees of protection. a mab to the se ... | 1988 | 2836547 |
| parainfluenza virus type 3 meningitis in an 11-month-old infant. | 1988 | 2835742 | |
| parainfluenza 3 virus and other common respiratory pathogens in children with human immunodeficiency virus infection. | 1988 | 2833716 | |
| [experiences with the riems vaccine combination pi-3 and riems ibr/ipv vaccines in cattle-raising stock]. | 1987 | 2831841 | |
| attenuation of bovine parainfluenza virus type 3 in nonhuman primates and its ability to confer immunity to human parainfluenza virus type 3 challenge. | bovine parainfluenza virus type 3 (piv-3) was evaluated as a candidate live-virus vaccine to protect against infection with human piv-3. the level of replication of bovine and human piv-3 and the efficacy of immunization with bovine piv-3 in protecting against subsequent challenge with human piv-3 was evaluated in nonhuman primates. the duration and magnitude of replication of human and bovine piv-3 in the upper and lower respiratory tracts of new world monkeys was similar, and animals infected ... | 1988 | 2831282 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, f and hn. | the sequence of the genes encoding the fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins of the human parainfluenza 3 virus was determined by molecular cloning. the genes were cloned by primer extension using genomic 50 s rna as the template. a series of four overlapping clones was generated from the 3' end of the fusion gene which extended across the gene end and intergenic boundaries of the f-hn and hn-l genes. the f gene extends 1851 nucleotides (inclusive of the putative transcri ... | 1987 | 2825443 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| [use of an immune blotting method (western) for studying viral antigens and for detecting antibodies to viral proteins]. | the technique of the procedure and the results of the use of immune ("western") blotting method for studies on viral antigens and detection of antibodies to individual proteins of viral particles are described. the possibility of detection and study of individual viral antigens in the whole plasma of patients or carriers is demonstrated by the example of hbs antigen of human hepatitis b virus. the method of immune blotting was used for screening of human sera for the detection of antibodies to t ... | 1987 | 2823482 |
| nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses. | the nucleotide sequence of the human parainfluenza virus 3 (hpiv3) hemagglutinin-neuraminidase (hn) gene has been determined using cdna clones derived from both hpiv3 genomic rna and mrna. the hn mrna contains 1,882 nucleotides, not including the poly(a) tail. primer extension experiments were carried out to locate the 5' terminal nucleotide of the hn mrna. the 3' end of the mrna was located at a putative polyadenylation signal. the hpiv3 hn mrna has one large open reading frame that codes for 5 ... | 1987 | 2822598 |
| rapid detection of influenza virus infections in human fetal lung diploid cell cultures. | haemadsorbing foci were found in human fetal lung (hfl) diploid cell cultures 12 h after inoculation with influenza viruses a and b. the size and number of the foci were maximal after 48 h of incubation, being limited by production of an unidentified inhibitor. by contrast, inoculation with parainfluenza virus type 3 led to haemadsorption which increased during 10 days of incubation. for the detection of influenza viruses a and b maximum sensitivity was achieved by changing the medium, the day b ... | 1989 | 2663998 |
| an evaluation of four serological tests for the detection of antibodies to bovine parainfluenza virus type 3. | haemagglutination-inhibition (hi), virus neutralization (vn), haemolysis-inhibition (hli-1, hli-2) tests, and two new haemofusion-inhibition (hfi-1, hfi-2) tests, developed by us, were tested for the detection of antibodies to bovine parainfluenza virus type 3 (bpiv-3) in sera of 45 steers randomly selected from a herd naturally infected by bpiv-3. twelve seronegative animals were then vaccinated with formalin-inactivated vaccine and 15 days later tested by the above-mentioned assays. linear reg ... | 1989 | 2561663 |
| studies of the effect of recombinant human-alpha 1 interferon on experimental parainfluenza type 3 virus infections of the respiratory tract of calves. | the effect of the administration of recombinant human interferon on the severity of clinical disease and the extent of pneumonic lesions in calves infected experimentally with bovine parainfluenza 3 (pi3) virus was studied in two experiments. in the first, three pairs of calves aged seven to 10 days were used; one of each pair was injected intramuscularly with 10(6) units of interferon/kg bodyweight for three consecutive days, and the other was left untreated. on the day after the first injectio ... | 1989 | 2559530 |
| parainfluenza 3 vaccination of sheep. | outbreaks of pneumonia associated with pasteurella haemolytica have occurred in sheep in the area of perthshire served by this practice, and on some farms the disease has been an important annual cause of loss. serological evidence was obtained that parainfluenza 3 (pi3) virus might be implicated as a predisposing factor to pasteurellosis. a live attenuated pi3 virus vaccine licensed for use in cattle was given intranasally to ewes on one farm. many sheep seroconverted and outbreaks of pneumonia ... | 1989 | 2556831 |
| cell lines for growth of sheep viruses. | 1989 | 2549933 | |
| association of parainfluenza virus type 3 infection with allograft rejection in a liver transplant recipient. | 1989 | 2549495 | |
| synthesis of 3-deazaneplanocin a, a powerful inhibitor of s-adenosylhomocysteine hydrolase with potent and selective in vitro and in vivo antiviral activities. | the neplanocin a analogue 3-deazaneplanocin a (2b) has been synthesized. a direct sn2 displacement on the cyclopentenyl mesylate 3 by the sodium salt of 6-chloro-3-deazapurine afforded the desired regioisomer 4 as the major product. after deprotection, this material was converted to 3-deazaneplanocin a in two steps. x-ray crystallographic analysis confirmed the assigned structure. consistent with its potent inhibition of s-adenosylhomocysteine hydrolase, 3-deazaneplanocin a displayed excellent a ... | 1989 | 2544721 |
| efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle. | efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ... | 1989 | 2542202 |
| analysis of the in vitro antiviral activity of certain ribonucleosides against parainfluenza virus using a novel computer aided receptor modeling procedure. | the in vitro antiviral activity of 28 nucleosides against the parainfluenza virus type 3 has been analyzed by using a novel computer aided receptor modeling procedure. the method involves an extensive modification of our earlier work (ghose, a. k.; crippen, g. m. j. med. chem. 1985, 28, 333). it presents a more straightforward algorithm for the steps that suffered from subjectivity in the earlier method. the method first determines the possible low-energy conformations of the nucleosides, and as ... | 1989 | 2539476 |
| reversal of virus-induced alveolar macrophage bactericidal dysfunction by cyclooxygenase inhibition in vitro. | virus infection of alveolar macrophages (am) both in vivo and in vitro has been associated with a decreased ability of these cells to kill bacteria, together with enhanced production of metabolites of arachidonic acid. these metabolites, especially pge2, may be inhibitory to some phagocyte functions. primary cultures of bovine am obtained by bronchoalveolar lavage of normal cattle were infected in vitro with parainfluenza-3 (pi3 virus) virus. killing of staphylococcus epidermidis by am was deter ... | 1989 | 2539425 |
| defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell. | a defective transport of bovine parainfluenza-3 virus (pi-3v) hemagglutinin-neuraminidase (hn) glycoprotein was evidenced in interferon (ifn)-treated bovine turbinate (btu) cells. indirect immunofluorescence performed with monoclonal antibody to pi-3 hn glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of ifn-treated cells. untreated, infected control cells had a generalized widespread fluorescence. unfixed control cells showed a uniform surface fluorescence in ... | 1989 | 2482015 |
| effects of bovine respiratory disease viruses and isoprinosine on bovine leukocyte function in vitro. | peripheral blood mononuclear cells obtained from 4- to 6-month-old-calves were inoculated in vitro with bovine herpesvirus-1, parainfluenza-3, or bovine virus diarrhea viruses. no increase in infectious virus progeny was observed; however, the viruses were detected in the cells for at least 96 h post-infection without any significant reduction in cell viability. the three viruses, either alone or in combination, suppressed phytohemagglutinin-induced proliferation of the mononuclear cells. the gr ... | 1989 | 2477940 |
| presence of an anti-viral factor in peritoneal dialysis effluent. | viral peritonitis is an exceptionally rare occurrence in peritoneal dialysis. in fact, up to now, only one case report has been documented in the literature. in a prospective study, peritoneal dialysis effluent (pde) was specifically cultured for the following viruses: the herpes group of viruses, including herpes simplex types i (hsv) and ii, cytomegalovirus (cmv) and varicella-zoster (v-z), and the enteroviruses group including coxsackie b-5 (cox b), echo, enterovirus and polio. cultures were ... | 1989 | 2476580 |
| [study of the multiplication in cell cultures of two strains of para-influenza virus type 3. iii. multiplication in bhk 21 cells]. | study was conducted on the multiplication of two strains (c243 and d) of parainfluenza virus type 3 in bhk 21 cells. multiplication curve of the virus was established and immunohistochemical aspects of the process were investigated. chronological study of successive steps of the formation and development of viral components allowed to see that the virus multiplication rate is low in this cell system. the parainfluenza antigen became detectable by immunofluorescence in the infected cell perinucle ... | 1987 | 2444030 |
| antigenic and structural properties of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3: sequence analysis of variants selected with monoclonal antibodies which inhibit infectivity, hemagglutination, and neuraminidase activities. | the hemagglutinin-neuraminidase (hn) gene sequence was determined for 16 antigenic variants of human parainfluenza virus type 3 (piv3). the variants were selected by using monoclonal antibodies (mabs) to the hn protein which inhibit neuraminidase, hemagglutination, or both activities. each variant had a single-point mutation in the hn gene, coding for a single amino acid substitution in the hn protein. operational and topographic maps of the hn protein correlated well with the relative positions ... | 1987 | 2437318 |
| characterization of four parainfluenza virus type 3 proteins by use of monoclonal antibodies. | monoclonal antibodies directed against four structural components of the atcc strain c243 of parainfluenza virus type 3 were produced. the specific reaction of the antibodies with individual structural components was determined by radioimmune precipitation assay. in the collection of monoclonal antibodies, 21 reacted with the haemagglutinin-neuraminidase (hn) glycoprotein (mol. wt. 72,000), eight with the fusion (f) glycoprotein (mol. wt. 64,000), 27 with the nucleocapsid (np) protein (mol. wt. ... | 1986 | 2426392 |
| antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. | sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (hn) protein of a 1957 isolate of parainfluenza type 3 virus (piv3) were produced and used to examine antigenic variation in clinical strains. analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in the presence of monoclonal antibodies indicated that there were a minimum of six distinct epitopes detectable on the hn molecule. competitive-binding assays indicated that these ep ... | 1985 | 2414910 |
| [a morphological study of pneumopathies caused by associated para-influenza and influenza viruses]. | histological examination of lungs collected from mice inoculated with influenza a/pr 8/34 (h0n0) and/or parainfluenza type 3 viruses revealed the existence of lymphohistiocytic interstitial bronchopneumonia lesions. necrosis zones were found in the lungs of influenza virus infected animals. associated infection by the two viruses induced also the fibroblast and fibrocyte proliferation, at same average ratios. | 1990 | 2176531 |
| parainfluenza-3 infection of the guinea pig airways induces respiratory airway hyperreactivity in vitro. | guinea pigs were inoculated intra-tracheally with bovine parainfluenza type 3 virus (pi-3) to investigate airway responsiveness to histamine and arecoline in vitro. two days after saline or pi-3 inoculation no difference in the reactivity of the tracheal spirals was observed after cumulative concentration/response curves with both drugs. similarly, contractions of parenchymal strips, induced by histamine, did not differ between both groups. however, 4 days after pi-3 inoculation the histamine in ... | 1990 | 2164762 |
| intracellular processing and transport of nh2-terminally truncated forms of a hemagglutinin-neuraminidase type ii glycoprotein. | six amino-terminal deletion mutants of the nh2-terminally anchored (type ii orientation) hemagglutinin-neuraminidase (hn) protein of parainfluenza virus type 3 were expressed in tissue culture by recombinant sv-40 viruses. the mutations consisted of progressive deletions of the cytoplasmic domain and, in some cases, of the hydrophobic signal/anchor. three activities were dissociated for the signal/anchor: membrane insertion, translocation, and anchoring/transport. hn protein lacking the entire c ... | 1990 | 2164031 |
| recombinant human interferon. | 1990 | 2155502 | |
| analysis of bovine parainfluenza-3 virus replication in bovine embryonic lung cells by indirect fluorescent antibody and hemadsorption assays. | the temporal manifestation of bovine parainfluenza-3 virus (bpi3v) proteins in the cytoplasm and on the surface of bovine embryonic lung (bel) cells was characterized by indirect fluorescent antibody (ifa) and hemadsorption (had) assays. intracellular proteins appeared earliest at 0.5 h post inoculation (p.i.) and the infection spread to virtually all cells by 48 h p.i. viral proteins on the surface of cells were seen first at 16 h p.i., and by 48 h p.i. the entire cell monolayer was ifa positiv ... | 1990 | 2155247 |
| [the incidence of exacerbations in chronic diseases in patients hospitalized with influenza and para-influenza]. | a retrospective analysis was made of the case reports of 829 patients admitted to the infectious department with a diagnosis of influenza (592) and parainfluenza (237). in all the patients, the diagnosis was supported serologically with the aid of the hemagglutination inhibition test, with the antibody titer in the serum rising 4-fold and more. in part of the patients the indicated test was used in combination with the immunofluorescence test in studying nasopharyngeal smears, whereas in part of ... | 1990 | 1965617 |
| evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | the toxicity and antiviral efficacy of carbocyclic 3-deazaadenosine (cc3ado) against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) virus infections were tested in tissue culture and in cotton rats. the mean median efficacious dose (ed50) of cc3ado in hep2 cells against rsv and piv3 was 9 and 14 micrograms/ml, respectively. these values were 85- and 55-fold less than the median inhibitory (toxic) dose (id50) of cc3ado in this cell line (750 micrograms/ml), and similar to values obta ... | 1990 | 1965109 |
| fusion properties of cells persistently infected with human parainfluenza virus type 3: participation of hemagglutinin-neuraminidase in membrane fusion. | cells persistently infected with human parainfluenza virus type 3 (hpf3) exhibit a novel phenotype. they are completely resistant to fusion with each other but readily fuse with uninfected cells. we demonstrate that the inability of these cells to fuse with each other is due to a lack of cell surface neuraminic acid. neuraminic acid is the receptor for the hpf3 hemagglutinin-neuraminidase (hn) glycoprotein, the molecule responsible for binding of the virus to cell surfaces. uninfected cv-1 cells ... | 1991 | 1851852 |
| infection of cultured human tracheal epithelial cells by human parainfluenza virus types 2 and 3. | despite growing information of the effects of human respiratory virus infection on airway physiology, little information is available on the mechanisms of pathology and pathophysiology in these infections. the human respiratory pathogens, parainfluenza virus types 2 and 3 (hpiv2, hpiv3, respectively), clinically cause laryngotracheobronchitis (infection of the large proximal airways). in order to examine the pathobiology of these viruses in airway cells of human origin, we exposed primary cultur ... | 1991 | 1849915 |
| numerous transitions in human parainfluenza virus 3 rna recovered from persistently infected cells. | the nucleotide sequences at the 3'-termini of human parainfluenza virus 3 (hpiv3) genomic rnas recovered from two lines of persistently infected llc-mk2 cells were determined following pcr amplification. after 29 months of persistence the 3'-end of the hpiv3 genome was found to be highly mutated. interestingly, the only types of nucleotide changes observed were u to c and a to g transitions. both u to c and a to g transitions were present on individual rna molecules. the data indicate that biase ... | 1991 | 1849685 |
| pathogenesis of human parainfluenza virus 3 infection in two species of cotton rats: sigmodon hispidus develops bronchiolitis, while sigmodon fulviventer develops interstitial pneumonia. | human parainfluenza virus 3 replicates well in the noses and lungs of two species of cotton rats, sigmodon hispidus and sigmodon fulviventer. peak viral titers of nearly 10(6) pfu/g are reached 2 days after infection in both tissues, are maintained through day 5, and are equivalent in the two species. infectious virus is eliminated by day 8 after infection. both species produce a strong neutralizing antibody response with titers of 1:10,000 4 weeks after infection. viral replication in the nasal ... | 1991 | 1845878 |
| homooligomerization of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 occurs before the acquisition of correct intramolecular disulfide bonds and mature immunoreactivity. | the posttranslational maturation of the hemagglutinin-neuraminidase (hn) glycoprotein of human parainfluenza type 3 virus (piv3) was investigated in pulse-chase experiments in which folding was monitored by immunoprecipitation with conformation-dependent antibodies and gel electrophoresis under nonreducing conditions and oligomerization was monitored by chemical cross-linking and sedimentation in sucrose gradients. the acquisition of mature immunoreactivity and the formation of correct intramole ... | 1991 | 1707981 |
| viral rna and protein synthesis in two llc-mk2 cell lines persistently infected with human parainfluenza virus 3. | two lines of llc-mk2 cells persistently infected with human parainfluenza virus 3 (hpiv-3) have been maintained in culture for approximately 3 years. subgenomic rnas (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. in one of the persistently infected cell lines cyclic variation in the production of virions containing standard virus genomic-size (50s) rna and subgenomic rna was observed. the molar ratio of subgenomic rna ... | 1990 | 1693460 |
| longitudinal study of acute respiratory diseases in rio de janeiro: occurrence of respiratory viruses during four consecutive years. | the occurrence of different viruses in nasopharyngeal secretions from children less than 5 years old with acute respiratory infections (ari) was investigated over a period of 4 years (1982-1985) in rio de janeiro. of the viruses known to be associated with ari, all but influenza c and parainfluenza types 1, 2 and 4 were found. viruses were found more frequently in children attending emergency or pediatric wards than in outpatients. this was clearly related to the high incidence of respiratory sy ... | 1991 | 1668974 |
| effects of zinc methionine and zinc oxide on performance, blood characteristics, and antibody titer response to viral vaccination in stressed feeder calves. | ninety steers with an average weight of 214 kg were purchased at 2 feeder calf sales and transported 70 to 100 km. on arrival at the feedlot, steers were weighed and identified, blood was withdrawn, and the steers were vaccinated against bovine herpesvirus-1 (bhv-1) and parainfluenza3 (pi3), using a modified live vaccine, and randomly assigned to treatment groups. treatments were: control (no supplemental zinc; zinc methionine; and zinc oxide. the control diet contained 26 mg of zinc/kg diet, an ... | 1991 | 1667527 |
| congenital hydrocephalus in suckling hamsters caused by transplacental infection with parainfluenza virus type 3. | the possible occurrence of congenital hydrocephalus by viral infection of the mother was examined by inoculating pregnant hamsters intravenously with parainfluenza virus type 3 (piv-3). we could produce congenital hydrocephalus in littermates born to a mother which had been inoculated into the left cervical vein on the 14th day of pregnancy. this result may indicate that the virus could pass through the placenta to arrive at the fetus, and infect the central nervous system. | 1991 | 1664659 |
| effects of 4-ipomeanol on bovine parainfluenza type 3 virus-induced pneumonia in calves. | previous research has demonstrated that 4-ipomeanol toxicosis can enhance the severity of para-influenza virus-induced pneumonia in mice. the objectives of this study were to determine whether calves are susceptible to 4-ipomeanol-induced enhancement of parainfluenza type 3 viral pneumonia and to determine whether 4-ipomeanol alters pulmonary replication of parainfluenza virus. male holstein calves were injected with either 4-ipomeanol (3 mg/kg) or vehicle (polyethylene glycol) 3 days prior to i ... | 1991 | 1661042 |
| protection of cotton rats by immunization with the human parainfluenza virus type 3 fusion (f) glycoprotein expressed on the surface of insect cells infected with a recombinant baculovirus. | the antigenicity, immunogenicity and efficacy of the human piv3 fusion (f) glycoprotein expressed in insect cells by a baculovirus vector were studied. the results indicate that the piv3 f glycoprotein expressed by a recombinant baculovirus is antigenically authentic as determined using a panel of piv3 f specific monoclonal antibodies. only a low level of antibody was stimulated by immunization of animals with infected cells, but the antibody appeared to be of high quality. immunized animals wer ... | 1991 | 1659054 |
| replication of parainfluenza type-3 virus and bovine respiratory syncytial virus in isolated bovine type-ii alveolar epithelial cells. | the objectives of our research were to determine whether bovine pulmonary type-ii alveolar epithelial cells could be isolated from bovine lung and maintained in tissue culture and to determine whether isolated bovine type-ii alveolar epithelial cells would support productive viral replication of bovine parainfluenza type-3 virus and bovine respiratory syncytial virus. type-ii alveolar epithelial cells were isolated from lungs of 4- to 7-day-old male holstein calves by enzymatic dissociation of p ... | 1991 | 1652906 |
| properties of human parainfluenza virus type 3 rna polymerase/replicase activity in vitro: consensus with other negative-stranded rna viruses. | a cell-free system supporting transcription, replication, and nucleocapsid assembly of the genome rna of human parainfluenza virus type 3 (hpf3) is described. cytoplasmic extracts from infected cv-1 or bhk cells catalyzed the transcription of the entire hpf3 genome, the replication of genome rna, and the assembly of this rna into nucleocapsidlike structures. newly replicated rna was resistant to micrococcal nuclease digestion and was stable in cscl gradients, exhibiting the density of authentic ... | 1991 | 1649342 |
| biological activity of paramyxovirus fusion proteins: factors influencing formation of syncytia. | the fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins of the paramyxovirus simian virus 5 (sv5) were expressed individually or coexpressed in cv-1 cells by using sv40-based vectors and recombinant vaccinia viruses. the extent of detectable fusion in a syncytium formation assay was found to be affected by the expression system used. in addition, when hn was coexpressed with f, it was found that the expression vector system influenced the contribution of hn in forming syncytia. the abi ... | 1992 | 1602561 |
| the nucleotide sequence of the l gene of marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses. | the nucleotide sequence of the l gene of marburg virus, strain musoke, has been determined. the l gene has a single long open reading frame encoding a polypeptide of 2330 amino acids (mw 267,175) that represents the viral rna-dependent rna polymerase. the putative transcription start signal (3'cuaccuauaauu 5') and the termination signal (3' uaauucuuuuu 5') of the gene could be identified. computer-assisted comparison of the l protein with l proteins of other nonsegmented negative-stranded rna vi ... | 1992 | 1546452 |
| [the improvement of the immunodiagnosis of para-influenza infection based on erythrocyte reagents]. | study of different methods of hemosensitization helped detect the optimum technology for the preparation of parainfluenza (type 3) antigenic erythrocyte diagnosticum. this technology ensures the highest sensitivity, specificity, economy (with respect to viral antigen consumption) and stability of the reagent. titration of antibodies by means of the new diagnosticum and the detection of parainfluenza cases among children with acute respiratory virus infections, acute and chronic glomerulonephriti ... | 1992 | 1379772 |
| virus-induced airway hyperresponsiveness in guinea pigs in vivo: study of broncho-alveolar cell number and activity. | recently, we demonstrated that an increased airway responsiveness in vitro can be measured 4, 8, and 16 days, but not 2 days, after intratracheal inoculation of parainfluenza-3 (pi-3) virus to guinea pigs. in the present study airway responsiveness was measured in vivo, and the number, types and activity of broncho-alveolar cells was determined. a significant increase in airflow resistance was measured in spontaneously breathing anesthetized guinea pigs in response to histamine and methacholine, ... | 1992 | 1335883 |
| [the effect of influenza and para-influenza on the course of ischemic heart disease]. | a retrospective analysis was made of 541 case reports of patients with coronary heart disease admitted to the infectious department with the diagnosis of influenza (n-387) and parainfluenza (n-154). in all the patients, the diagnosis was verified serologically with the aid of the hemagglutination inhibition test, with a 4-fold and greater increase of the antibody titer in the serum. in part of the patients, it was confirmed by the above test combined with immunofluorescence in examining rhinopha ... | 1992 | 1332205 |
| the complete nucleotide sequence of the js strain of human parainfluenza virus type 3: comparison with the wash/47885/57 prototype strain. | the nucleotide sequence of the js strain of human parainfluenza virus type 3 (piv3) was determined from a series of 14 overlapping cdna clones and was compared to that of the previously sequenced prototype piv3 strain, wash/47885/57 (galinski, 1991). overall, there were 630 (4%) nucleotide differences between the two viruses. 15462 nucleotides comprised the js genome in contrast to 15463 which constituted the genome of the prototype virus. this was accounted for by a single nucleotide deletion i ... | 1992 | 1329377 |
| isolation of parainfluenza virus type 3 from cerebrospinal fluid. | 1992 | 1316416 | |
| [research on substances with antiviral activity. x. 1-substituted-2-(methylamino) naphtho/2,1-b/furan hydrochlorides]. | some 2-(methylamino)naphtho [2,1-b] furans hydrochlorides 1-substituted were synthetized and investigates for antiviral activity in vitro against herpes simplex virus type i (vhs-i) and parainfluenza virus type 3 ha-i/cr-8 stock. | 1979 | 233363 |
| the experimental production of pneumonia in calves by intranasal inoculation of parainfluenza type iii virus. | thirteen colostrum deprived calves were inoculated with a low passage field strain of parainfluenza type iii (pi 3) virus. virus was administered by the intranasal route and the calves were experimentally infected twice daily for four consecutive days. clinical signs of respiratory disease were noted and pneumonia was present in 12 animals at necropsy. pi 3 virus was isolated from the lung lesions and no other respiratory pathogens could be demonstrated. the pneumonic lesions grossly, histologic ... | 1979 | 231340 |
| characterization of bovine parainfluenza virus type 3. | bovine parainfluenza virus type 3 (piv-3) has a buoyant density of 1.197. the rna of piv-3, like that of sendai virus, is a single continuous chain which lacks polyadenylic acid sequences and tends to self-anneal to a marked extent. it has a sedimentation coefficient of 42s and a molecular weight of 4.5 x 10(6), being slightly smaller than sendai virus rna (47s, 5.3 x 10(6)). piv-3 has 5 main structural proteins, of which 2 are glycoproteins. the molecular weights of protein 1, protein 2, protei ... | 1979 | 228163 |
| pulmonary cell-mediated cytotoxicity in hamsters with parainfluenza virus type 3 pneumonia. | a 51cr-release cytotoxic assay in vitro was developed to permit definition of the role of local pulmonary cell-mediated immunity in the recovery phase of experimental parainfluenza virus type 3 pneumonia in the syrian hamster. cytotoxic effector cells were obtained by bronchalveolar lavage; virus-infected targets were syngeneic secondary hamster kidney cells. maximal target-cell killing was mediated by lung lavage cells obtained one week after infection; the response waned rapidly thereafter. th ... | 1979 | 223482 |
| serological evidence of infectious bovine rhinotracheitis, bovine virus diarrhoea and parainfluenza-3 viruses in calves in el salvador. | 1979 | 223256 | |
| [experimental model of associated influenza-para-influenza infection]. | an experimental model of associated influenza-parainfluenza infection has been developed. simultaneous inoculation of mice with influenza a2/21/65 and parainfluenza type 3 or inoculation with these viruses at an interval of 24 hours was shown to produce a considerably more severe disease as manifested by the development of severe confluent pneumonias involving both lungs and death of the inoculated animals. the animals with the associated infection showed no significant difference in antibody ti ... | 1978 | 219615 |
| giant-cell pneumonia caused by parainfluenza virus. | 1979 | 217981 | |
| fatal rhabdomyolysis associated with parainfluenza type 3 infection. | 1978 | 213404 | |
| ovine pneumonia. | 1978 | 210432 | |
| experimental infection of specific pathogen-free lambs with parainfluenza virus type 3 and pasteurella haemolytica. | 1978 | 207749 | |
| an inactivated parainfluenza virus type 3 vaccine: the influence of vaccination regime on the response of calves and their subsequent resistance to challenge. | calves maintained in insolated pens were vaccinated with an inactivated parainfluenza virus type (3) (pi3) vaccine usingparenteral and local route singly and in combination. the calves were subsequently monitored for serum antibody response and challenged intranasally with live virus to assess the protection derived from vaccination. calves receiving one subcutaneous dose of vaccine in oil adjuvant produced a marked antibody response and were partially protected against challenge. those receivin ... | 1978 | 206935 |
| immunologic "memory" for microbial antigens in lymphocytes obtained from human bronchial mucosa. | memory for previous immunologic contact with microbial antigens has been detected in lymphocytes from human bronchi as a secondary immune response, when tested in vitro. antigens stimulated a predominantly proliferative response in blood lymphocytes that was significantly greater than the response in mucosal lymphocytes with purified protein derivative and herpes simplex type 1 antigens. co-culture experiments with autologous blood lymphocytes showed that cell-dependent suppression was one mecha ... | 1978 | 204236 |
| [detection of the parainfluenza virus in respiratory pathology in lambs]. | 1978 | 204101 | |
| investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program. | serum samples were collected from early weaned fall calves shortly after the onset of respiratory tract disease. antibody titers to infectious bovine rhinotracheitis (ibr) virus, parainfluenza type 3 (pi-3) virus, bovine viral diarrhea (bvd) virus, bovine adenovirus type 3 (bav-3), and bovine respiratory syncytial virus (brsv) were determined on paired (acute and convalescent) serums. seroconversion rate (a fourfold or greater rise in antibody titer) for ibr virus was 4.3%, pi-3 virus--16.3%, bv ... | 1977 | 201196 |
| [clinical and immunopathological studies of viral nephritis]. | 1977 | 199763 | |
| components of parainfluenza-3 virus, slp-strain, reacting in assays of cell-mediated immunity in cattle. | parainfluenza-3 virus was isolated by affinity chromatography, including a purification step with immobilized lectin vicia erwilia. the peplomers of disintegrated virus were similarily isolated using another carbohydrate-specific lectin vicia crotalaria. the whole virion and the peplomers were both active as antigens in the leucocyte migration inhibition, lymphocyte stimulation and skin hypersensitivity tests. the remaining virus material, freed of detergents used for virus disintegration and co ... | 1977 | 194552 |
| immunoglobulins in the serum and nasal secretions of lambs following vaccination and aerosol challenge with parainfluenza 3 virus. | serial changes in the concentrations of igm, igg and iga were compared in specific pathogen free (spf) lambs which had been vaccinated with live or inactivated parainfluenza 3 virus (pi 3) by either intramuscular (im) or intranasal (in) routes followed by aerosol challenge with pi 3. in the serum, an increase in igm was associated with the primary antibody response to the aerosol challenge, whereas increased igg was associated with the secondary antibody response. no changes in immunoglobulin co ... | 1976 | 193171 |