Publications

implication of human papillomavirus-66 in vulvar carcinoma: a case report.	abstract:	2011	21702970
fathers' intentions to accept human papillomavirus vaccination for sons and daughters: exploratory findings from rural honduras.	objectives: little is known about fathers' attitudes toward human papillomavirus (hpv) vaccination in low-resource settings. we sought to determine the awareness of hpv vaccination among honduran fathers, and to assess their intention to accept hpv vaccination for their sons and daughters. methods: we conducted 100 structured interviews of fathers recruited from medical and business settings between may 2007 and june 2008. after assessing baseline knowledge, fathers received a brief explanation ...	2011	21698492
detection and genotyping of human papilloma virus in cervical cancer specimens from saudi patients.	to determine the rates and types of human papilloma virus (hpv) infection in cervical cancer specimens from saudi patients.	2011	21697680
the e7 open reading frame acts in cis and in trans to mediate differentiation-dependent activities in the human papillomavirus type 16 life cycle.	human papillomaviruses (hpvs) are the causative agents of several important genital and other mucosal cancers. the hpv16 e7 gene encodes a viral oncogene that is necessary for the continued growth of cancer cells, but its role in the normal, differentiation-dependent life cycle of the virus is not fully understood. the function of e7 in the viral life cycle was examined using a series of mutations of e7 created in the context of the complete hpv16 genome. the effect of these e7 mutations on key ...	2011	21697473
human papillomavirus 13 in a mexican mayan community with multifocal epithelial hyperplasia: could saliva be involved in household transmission?	multifocal epithelial hyperplasia (meh) is a disease of the oral mucosa. human papillomaviruses 13 and 32 have been detected in these lesions. we describe the epidemiology and clinical characteristics of patients with meh in a rural community in the mayan area of mexico with 53 cases and 54 controls. clinical and epidemiological data were collected through a direct interview. oral cell samples were collected with a cytobrush. subjects collected their own saliva sample in a sterile bottle. all sa ...	2011	21697033
associations of human leukocyte antigen class ii genotypes with human papillomavirus 18 infection and cervical intraepithelial neoplasia risk.	background: only a small proportion of women infected with human papillomavirus type 18 (hpv18) may progress to persistent infection and cervical neoplasia. this community-based cohort study aimed to assess associations with human leukocyte antigen (hla) class ii genotypes for natural infection of hpv18 and subsequent risk of cervical neoplasia. methods: among 10,190 cytologically normal participants, 125 with hpv18 infection were identified by hpv blot kit. hpv18 viral load at study entry was e ...	2011	21692064
why do young women get tested for sexually transmitted infections? evidence from the national longitudinal study of adolescent health.	abstract purpose: public health officials promote sexually transmitted infection (sti) testing as a method to reduce the morbidity of stis. the purpose of this study was to examine what factors are associated with sti testing among young women across various types of stis and to compare relative influences of factors across models. methods: a secondary data analysis of data from add health wave iii was conducted (n=2629). explanatory factors highlighted in qualitative literature were operational ...	2011	21689024
the strong protective effect of circumcision against cancer of the penis.	male circumcision protects against cancer of the penis, the invasive form of which is a devastating disease confined almost exclusively to uncircumcised men. major etiological factors are phimosis, balanitis, and high-risk types of human papillomavirus (hpv), which are more prevalent in the glans penis and coronal sulcus covered by the foreskin, as well as on the penile shaft, of uncircumcised men. circumcised men clear hpv infections more quickly. phimosis (a constricted foreskin opening impedi ...	2011	21687572
Human papillomavirus (HPV) detection using in situ hybridization in histologic samples: correlations with cytologic changes and polymerase chain reaction HPV detection.	Although in situ hybridization (ISH) and polymerase chain reaction (PCR) have extensively been used on cytology specimens, there have been limited reports of the usefulness of these techniques in relation to confirmed histologic findings. In this study, we used PCR and ISH to detect human papillomavirus (HPV) in cytologic and histologic specimens, respectively. By using positive and negative likelihood ratios, we attempted to identify any predictive role of ISH testing alone or in combination wi ...	2011	21685039
human papillomavirus vaccination in the resourced and resource-constrained world.	human papillomavirus has been established as the causal agent for cervical cancer. the identification of a clear cause presents an unparalleled opportunity for cancer control. as such, the development of prophylactic human papillomavirus vaccines has been rightly hailed as one of the significant scientific triumphs of the past 20 years. this story of scientific triumph over disease, however, is not yet complete. the fruit of scientific labour must be delivered to the people in order to fulfil th ...	2011	21684811
early effect of the hpv vaccination programme on cervical abnormalities in victoria, australia: an ecological study.	australia introduced a human papillomavirus (hpv) vaccination programme with the quadrivalent hpv vaccine for all women aged 12-26 years between 2007 and 2009. we analysed trends in cervical abnormalities in women in victoria, australia, before and after introduction of the vaccination programme.	2011	21684381
implementation of e-bug in denmark.	bacterial resistance to antimicrobial agents in primary healthcare is low in denmark compared with most other european countries. denmark has a vaccination programme for children that recommends immunization against 10 infectious diseases, including a heptavalent pneumococcal conjugate vaccine (pcv7) and a vaccine against human papillomavirus (hpv). outbreaks in schools due to other infectious illnesses are well recognized and have seasonal variations. a quarter of young people have had a sexual ...	2011	21680590
value of hpv-dna test in women with cytological diagnosis of atypical glandular cells (agc).	objective: this study analyzed whether hpv (human papillomavirus) testing contributes towards defining histological abnormalities in women with atypical glandular cells (agc) diagnosed at cervical cytology. study design: one hundred and eight women with conventional cervical cancer screening smears suggestive of agc not otherwise specified (agc-nos) and favor neoplastic (agc-fn) were consecutively enrolled. all women underwent colposcopic examinations and biopsy was performed according to the cy ...	2011	21680079
low-level persistence of human papillomavirus 16 dna in a cohort of closely followed adolescent women.	most human papillomavirus (hpv) infections in young women become undetectable by standard assays after a few months. it is possible that many hpv infections do not actually clear, but persist at very low levels for years, becoming detected again later in life. the purpose of this study is to describe hpv 16 clearance, reappearance, and low-level persistence in a cohort of adolescent women. adolescent women (nôçë=ôçë66), not vaccinated against hpv, were recruited from 1998 to 2008 into a longitud ...	2011	21678439
evolution and taxonomic classification of human papillomavirus 16 (hpv16)-related variant genomes: hpv31, hpv33, hpv35, hpv52, hpv58 and hpv67.	human papillomavirus 16 (hpv16) species group (alpha-9) of the alphapapillomavirus genus contains hpv16, hpv31, hpv33, hpv35, hpv52, hpv58 and hpv67. these hpvs account for 75% of invasive cervical cancers worldwide. viral variants of these hpvs differ in evolutionary history and pathogenicity. moreover, a comprehensive nomenclature system for hpv variants is lacking, limiting comparisons between studies.	2011	21673791
postlicensure safety evaluation of human papilloma virus vaccines.	a summary of postlicensure safety information of hpv vaccines from three sources: vigibase, the global database of who's programme for international drug monitoring, the vaers report on gardasil and the rivm report on cervarix.	2011	21673418
serum antibody response following genital {alpha}9 human papillomavirus infection in young men.	background.ôçâalthough the prevalence of human papillomavirus (hpv) genital infection is similarly high in males and females, seroprevalence is lower in males. this study assessed rates and determinants of seroconversion after detection of genital hpv infection in young men. methods.ôçâwe investigated hpv type-specific seroconversion in a cohort of heterosexual male university students who had an ╬▒9 hpv type (hpv-16, -31, -33, -35, -52, -58, or -67) detected in the genital tract (n = 156). hpv ...	2011	21673030
human papillomavirus genotype distribution among french women with and without cervical abnormalities.	to assess human papillomavirus (hpv) prevalence and distribution among french women with normal and pathologic cytology findings.	2011	21669421
racial disparities in awareness of the human papillomavirus.	abstract background: human papillomavirus (hpv) is the most common sexually transmitted infection in the united states yet is one of the least recognized among the general public. hpv awareness may be relatively low among minority women. because hpv awareness is associated with uptake of the hpv vaccine, it is critical to assess hpv awareness in the population and identify any racial/ethnic gaps. methods: this study used nationally representative data collected by the national survey of family g ...	2011	21668381
higher micronutrient intake is associated with human papillomavirus-positive head and neck cancer: a case-only analysis.	no studies have investigated dietary differences between head and neck squamous cell carcinoma (hnscc) patients with human papillomavirus (hpv)-positive tumors and patients with hpv-negative tumors. this study was designed to investigate the relationship between diet and hpv status in hnscc patients. cases of hnscc were recruited from 2 clinical centers participating in the university of michigan head and neck specialized program of research excellence (spore). hpv tissue genotyping was performe ...	2011	21667401
transforming activity of a novel mutant of hpv16 e6e7 fusion gene.	an optimized recombinant hpv16 e6e7 fusion gene (hpv16 ofe6e7) was constructed according to codon usage for mammalian cell expression, and a mutant of hpv16 ofe6e7 fusion gene (hpv16 omfe6e7) was generated by site-directed mutagenesis at l57g, c113r for the e6 protein and c24g, e26g for the e7 protein for hpv16 ofe6e7 [patent pending (cn 101100672)]. the hpv16 omfe6e7 gene constructed in this work not only lost the transformation capability to nih 3t3 cells and tumorigenicity in scid mice, but a ...	2011	21667341
the role of human papillomavirus on sperm function.	to review the role of human papillomavirus (hpv) on sperm parameters, fertility and implication of the use of infected sperm cells in assisted reproduction.	2011	21666465
human papillomavirus dna and mrna positivity of the anal canal in women with lower genital tract hpv lesions: predictors and clinical implications.	women with hpv related pathology of the lower genital tract are at higher risk for ain and anal cancer than the general population. a strategy to identify anal disease in these women has not been formulated. the aim of this study is to examine the feasibility of hpv related biomarker testing on anal smears, to identify the risk factors for anal hpv positivity and to provide information of the clinical implications of anal hpv infection in this population.	2011	21665253
new technologies and advances in colposcopic assessment.	to have a good grasp of clinical colposcopy, it is necessary to understand the histopathologic structure of the normal and dysplastic cervical epithelium. previous meta-analyses had indicated high overall sensitivity of colposcopy in detecting dysplastic lesions, but recent studies have suggested that the technique has much lower sensitivity in detecting high-grade intraepithelial neoplasia. the best practice in colposcopy relies on accurately taking a biopsy from the correct (i.e. most morpholo ...	2011	21664876
meeting of the who human papillomavirus vaccine advisory committee, april 2010.		2011	21661272
[investigation on hpv dna detection and genotyping practices used in french laboratories in 2009].	the french national reference laboratory for human papillomavirus (hpv) performed in 2009 a national study in order to review the methods used to detect and identify hr hpv genotypes in microbiology laboratories. results from this study show a great diversity in volumes of samples treated in laboratories. among clinical indications, the most frequent is a result of asc-us at a pap smear. this indication in the only one covered by the national public insurance system and is mostly performed in la ...	2011	21659046
adult immunizations: update on recommendations.	the advisory committee for immunization practices recommends universal influenza vaccination for 2010-2011. older adults should be offered protection against herpes zoster, and younger adults should receive immunization against human papilloma virus and pertussis. hepatitis b vaccination should be encouraged in non-immune adults. recommendations also address vaccinations for tetanus/diphtheria, hepatitis a, pneumococcus, measles/mumps/rubella, and meningococcus.	2011	21658665
prognostic implication of high risk human papillomavirus e6 and e7 mrna in patients with intraepithelial lesions of the cervix in relationship to age.	since the introduction of the cytological screening programs, a significant reduction in the incidence of cervical cancer has been achieved. almost all of these cancers are related to high-risk (hr) human papillomavirus (hpv) cervical infections. however, the natural history of hpv infection seems to be different in younger patients, resulting in a higher rate of regression. there is, therefore, the need to identify hpv-related biomarkers in order to enhance the effectiveness of screening of hig ...	2011	21658320
pcr study of a series of ascus cases hpv-positive by hcii.	most guidelines currently recommend the testing of human papillomavirus (hpv) in ascus cases. the most used method for this purpose is hybrid capture ii (hcii), but pcr techniques with gp5+/6+ primers can be also applied. furthermore, the hcii high-risk probe test for detection of hpv shows cross-reactivity with low-risk hpv. although this cross-reactivity has been studied in screening populations, it has received little attention in ascus cases. to compare the performance of the hcii high-risk ...	2011	21656701
serum antibodies to the hpv16 proteome as biomarkers for head and neck cancer.	human papillomavirus (hpv) type 16 is associated with oropharyngeal carcinomas (opc). antibodies (abs) to hpv16 e6 and e7 oncoproteins have been detected in patient sera; however, abs to other early hpv-derived proteins have not been well explored.	2011	21654689
providing vaccines against human papillomavirus to adolescent girls in the americas: battling cervical cancer, improving overall health.	vaccines against the human papillomavirus (hpv)--the primary cause of cervical cancer--target adolescent girls, many of whom have limited contact with health services. countries in the americas are beginning to use hpv vaccines to increase the impact of cervical cancer programs and as an entry point to broader health services for girls. this strategy opens new opportunities to improve lifelong health habits; encourage regular cervical cancer screening and treatment, when necessary; and offer ass ...	2011	21653962
antitumor therapeutic and antimetastatic activity of electroporation-delivered human papillomavirus 16 e7 dna vaccines: a possible mechanism for enhanced tumor control.	dna vaccines are known to be lacking in immunogenicity in humans. presently, electroporation (ep) is thought to overcome this limitation. here, we investigate whether human papillomavirus 16 e7 dna vaccines delivered by ep might elicit potent antitumor activity in animal cervical cancer models, with a focus on the underlying mechanism(s). intramuscular (im)-ep delivery of e7 dna vaccines induced more potent antitumor therapeutic and antimetastatic activity compared with im delivery. moreover, th ...	2011	21649506
quadrivalent papillomavirus vaccine in women over age 26. cervical screening must remain a priority.		2011	21648175
hpv protection in older groups may be in the eye of the beholder.		2011	21647126
the epidemic of hpv-associated oropharyngeal cancer is here: is it time to change our treatment paradigms?	although relatively uncommon, oropharyngeal cancers are increasing in incidence despite declining prevalence of smoking and in direct opposition to a decreasing incidence of all other head and neck cancers. an epidemic of human papillomavirus (hpv)-associated oropharyngeal cancers seems to account for these incidence trends. important demographic, behavioral, and prognostic characteristics define this unique population. changes in prevention, diagnosis, evaluation, staging, and treatment are nee ...	2011	21636538
reduced mir-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting plk1 protein.	aim: although aberrant mirnas expression has been documented, altered mir-100 expression in cervical cancer and precursor tissues and its carcinogenic effect and mechanism remain unexplored. the aim of our study was to investigate the role of mir-100 alteration in cervical carcinogenesis. methods: the expression of mir-100 was examined by quantitative real-time reverse transcriptase pcr (qrt-pcr) in 125 cervical tissues including normal cervical epithelium, cervical intraepithelial neoplasia (ci ...	2011	21636267
oncogenic potential of human papillomavirus (hpv) and its relation with cervical cancer.	abstract: human papillomavirus (hpv) is the most common cause of cervical cancer. cervical cancer being the second most common cancer after lung cancer, affecting women of different age groups; has a prevalence of about 20% in young sexually active women. among different types of hpv, hpv16 the major strain causing this cancer and is sexually transmitted had been unnoticed for decades. keeping in mind the multiple risk factors related with cervical cancer such as early age sexual activities, tee ...	2011	21635792
an evaluation of a new dna test for carcinogenic human papillomavirus.	using archived specimens, we evaluated a new automated real-time pcr assay (bd diagnostics), which detects all carcinogenic hpv and provides hpv genotyping for seven of them, including hpv16 and hpv18, the two most carcinogenic hpv genotypes. we found comparable results with hybrid capture 2 (hc2) for detection of carcinogenic hpv (n = 473) and with linear array and line blot assay (n = 371) for detection of individual hpv genotypes.	2011	21632892
hpv vaccine against hpv infection and disease in males.		2011	21631334
improved sensitivity of vaginal self-collection and high-risk human papillomavirus testing.	background: self-collected vaginal specimens tested for high-risk human papillomavirus (hr-hpv) have been shown to be less sensitive for detection of cervical intraepithelial neoplasia or cancer (≥cin 3) than physician-collected endocervical specimens. to increase the sensitivity of self-collected specimens, we studied a self-sampling device designed to obtain a larger specimen from the upper vagina (poi/nih self-sampler) and a more sensitive pcr based hr-hpv assay. materials and methods: 10,000 ...	2011	21630255
a competitive serological assay shows naturally acquired immunity to human papillomavirus infections in the guanacaste natural history study.	background. a competitive luminex immunoassay (clia) has been developed to measure neutralizing antibodies against human papillomavirus (hpv) types 6, 11, 16 and 18. methods. in a cohort of 974 women from the guanacaste natural history study, we studied the relationship of baseline clia and virus-like particle (vlp) enzyme-linked immunosorbent assay (elisa) (hpv16 and hpv18 only) seropositivity to measures of hpv exposure, hpv dna positivity, number of sexual partners, cytology findings, and age ...	2011	21628663
detection of high-risk human papillomavirus types 16 and 18 but not 33 and 52 in external genital warts from iranian females.	background: external genital warts (egw) are relatively common sexually transmitted diseases. in the majority of cases, low-risk human papilomaviruses (hpv), such as hpv-6 and hpv-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical cancer prevention. in this study the incidence of the high-risk hpv types 16, 18, 33 and 52 in egws of females from the southwest of iran was assessed. methods: seventy-nine women with egws participated in this ...	2011	21627381
prevalence and distribution of high risk human papillomavirus (hpv) types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in the general population in karnataka, india.	background: in view of conducting hpv vaccination in india it is most important to understand the prevalence of hpv genotypes in this population, not only in squamous cell carcinoma of cervix and oral cavity but also in the general population. in this study we explored the prevalence and distribution of high-risk hpv types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in general population in karnataka. methods: cervical cancer specimens after punch b ...	2011	21627358
incidence of cervical intraepithelial neoplasia grade 2 or worse in colposcopy-negative/human papillomavirus-positive women with low-grade cytological abnormalities.	please cite this paper as: kelly r, walker p, kitchener h, moss s. incidence of cervical intraepithelial neoplasia grade 2 or worse in colposcopy-negative/human papillomavirus-positive women with low-grade cytological abnormalities. bjog 2011; doi: 10.1111/j.1471-0528.2011.02970.x objective to determine the risk of incident high-grade cervical intraepithelial neoplasia (cin) in human papillomavirus (hpv) -positive women with low-grade cytological abnormalities who had a satisfactory normal colp ...	2011	21624034
clinicopathologic correlations between human papillomavirus 16 infection and beclin 1 expression in human cervical cancer.	our earlier study showed that the autophagy gene beclin 1 could affect cell proliferation in a cervical cancer hela cell line. in this study, we examined beclin 1 protein expression in 81 specimens of cervical squamous carcinoma by immunohistochemistry. meanwhile, we detected e6 and e7 genes of human papillomavirus 16 in these tissues by polymerase chain reaction. beclin 1 expression significantly decreased in samples of malignant cervical cancer tissues than in those of normal or cervical intra ...	2011	21623196
hpv vaccine information-seeking behaviors among us physicians: government, media, or colleagues?	the multiple information sources available may pose a challenge to physicians in providing accurate human papillomavirus (hpv) vaccine information to patients. the purpose of this study was to describe physicians' hpv vaccine information-seeking behaviors and assess if these behaviors differ by physician specialty and sociodemographic characteristics. in 2009, 1008 family physicians (fps), pediatricians (peds), and obstetricians/gynecologists (obgyns) completed a survey to assess their hpv vacci ...	2011	21619906
improved genetic association tests for an ordinal outcome representing the disease progression process.	we are interested in detecting genetic variants that influence transition between discrete stages of a disease progression process, such as the natural history of progression to cervical cancer with the following four stages: (1) normal-human papillomavirus (hpv) exposed, (2) persistent infection with oncogenic hpv, (3) cervical intraepithelial neoplasia grades 2 or 3 (cin2/3), and (4) cervical cancer. standard statistical tests derived from the proportional odds model or polytomous regression m ...	2011	21618605
distribution of human papillomavirus genotypes in paraguayan women according to the severity of the cervical lesion.	the incidence of cervical cancer in paraguay is among the highest in the world. this study aimed to determine the distribution of human papillomavirus (hpv) genotypes in paraguayan women, according to the severity of the cervical lesion. this cross-sectional study included 207 women without a squamous intraepithelial lesion, 164 with low-grade squamous intraepithelial lesions, 74 with high-grade squamous intraepithelial lesions, and 41 with cervical cancer. type-specific hpv was determined by th ...	2011	21618554
human papillomavirus genotypes among infected thai women with different cytological findings by analysis of e1 genes.	cervical cytological data may not be sufficient for cervical cancer screening and prevention. in this project, we determined hpv genotype among infected thai women with different cytological findings by characterization of e1 genes. five hundred and thirty-five specimens were tested by pcr amplification of the e1 genes. hpv genotypes were determined by sequencing, comparison with the genbank database and were analyzed in relation to different cytological findings. hpv-dna by pcr were typed and r ...	2011	21617826
human papillomavirus (hpv) vaccination: perception and practice among french general practitioners in the year since licensing.	acceptance of the human papillomavirus (hpv) vaccine by targeted populations will depend to a large extent on its acceptability among physicians. we examined the perceptions, attitudes and practices of general practitioners (gps) in relation to hpv vaccination. from november 2007 to april 2008, a cross-sectional survey was carried out among a representative 5% sample of gps in the large rhône-alpes region of france. both quantitative (self-administered questionnaire) and qualitative (interview) ...	2011	21616114
a systemic review of human papillomavirus studies: global publication comparison and research trend analyses from 1993 to 2008.	the term "human papillomavirus" has been used as the keyword during searching titles, abstracts, and keywords based on the online version of science citation index (sci), web of science from 1993 to 2008. twelve document types were found among the 14,943 papers published in 1,072 journals that were listed in 99 sci subject categories. all the articles referring to human papillomavirus were assessed by using the following aspects: characteristics of publication output, distribution of output in j ...	2011	21614898
[detection of human papilloma virus (hpv) in liquid-based cervical samples. correlation with protein p16ink4a expression].	the liquid-based cervical cytology improves the quality of the sample and the residual sample could be used efficiently to carry out complementary tests, such as the detection of hpv dna and the immunocytochemical biomarkers study. the purpose of this study was to correlate the presence of hpv and immunoexpression of p16ink4a in liquid-based cervical samples to examine the utility of these new tools in the detection of cervical cancer. the included patients (n = 67) presented an abnormal cytolog ...	2011	21614811
prevalence of human papillomavirus, epstein-barr virus, p21, and p53 expression in sinonasal inverted papilloma, nasal polyp, and hypertrophied turbinate in hong kong patients.	background: the purpose of this study of human papillomavirus (hpv), epstein-barr virus (ebv), p21, and p53 in sinonasal inverted papilloma (ip) was to help elucidate its pathogenesis. methods: seventy-three ips, 48 nasal polyps, and 85 hypertrophied turbinates were subjected to hpv polymerase chain reaction (pcr) study. seventy-three ips, 30 nasal polyps, and 32 hypertrophied turbinates were subjected to ebv in situ hybridization (ish), p21, and p53 immunohistochemical (ihc) studies. results: h ...	2011	21608063
interrelationship between the expression of h-ras p21, c-myc and p53 proteins, and the infection of high-risk human papillomaviruses in japanese patients with esophageal squamous-cell carcinoma.	the presence and distribution of increased expression of h-ras p21 protein, c-myc protein and p53 protein, or of human papillomavirus (hpv) dna were investigated in 42 japanese patients with esophageal squamous cell carcinoma (scc) by immunohistochemical techniques using each protein antibody, and by in situ hybridization with fluorescein isothiocyanate (fitc)-labelled dna. probes for hpv-16 and hpv-18. eighteen cases were positive with h-ras p21 antibody, but only one with c-myc protein antibod ...	1994	21607450
human papillomavirus testing in primary cervical screening and the cut-off level for hybrid capture 2 tests: systematic review.	to determine the trade-off between the sensitivity and the specificity for high grade cervical intraepithelial neoplasia at hybrid capture 2 cut-off values above the standard ≥1 relative light units/cut-off level (rlu/co).	2011	21606136
a long-term prospective study of type-specific human papillomavirus infection and risk of cervical neoplasia among 20,000 women in the portland kaiser cohort study.	background. human papillomavirus (hpv) dna testing is more sensitive than cytology for detection of cervical intraepithelial neoplasia grade 3 and cancer (>cin3). adding hpv testing to cytology is recommended for women >30 but long-term prospective studies of hpv testing are rare. methods. beginning in 1989-1990, ~20,000 women in a pre-paid health maintenance organization (median age = 34) were followed passively by recommended annual cytology. we tested archived cervicovaginal lavage specimens ...	2011	21602310
magnetic microparticle-based multiplexed dna detection with biobarcoded quantum dot probes.	we have developed a new analytical method to detect multiple dna simultaneously based on the biobarcoded cdse/zns quantum dot (qd) and magnetic microparticle (mmp). it was demonstrated by using oligonucleotide sequences of 64 bases associated with human papillomavirus 16 and 18 l1 genes (hpv-16 and hpv-18) as model systems. this analytical system involves three types of probes, a mmp probe and two streptavidin-modified qd probes. the mmps are functionalized with hpv-16 and hpv-18 captures dna to ...	2011	21602038
follow-up outcomes for a large cohort of us women with negative imaged liquid-based cytology findings and positive high risk human papillomavirus test results.	objective: this study aimed to follow a large group of us women with negative computer-imaged liquid-based cytology (lbc) and positive high risk (hr) hpv dna results. methods: negative lbc and positive hrhpv cases were identified between july 1, 2005 and december 31, 2009. cytologic and histopathologic follow-up results, repeat hpv results, and prior history were analyzed. results: 1099 patients with negative lbc and positive hrhpv results were identified. eight hundred sixty-nine had repeat pap ...	2011	21601911
knowledge of hpv in west virginia high school health students and the effects of an educational tool.	study objective: to assess the knowledge of human papillomavirus (hpv) in west virginia high school students, create an educational dvd that could be used as a supplement to health class, and evaluate whether our intervention increases student knowledge of hpv. design: prospective, randomized experimental design with analysis of questionnaire data. setting: west virginia high school health class. participants: a total of 626 students participated. interventions: hpv educational dvd designed by h ...	2011	21600809
[significance for the detection of human papillomavirus dna in cervical atypical glandular cells.]	objective: to evaluate the potential link between high-risk human papilloma virus (hpv)dna testing and histological diagnosis in women referred for atypical glandular cells (agc) at pap smear. methods: all cervical cytological examinations with the diagnosis of agc between january 2007 and dec 2009 were identified by means of a computerized database in 97 patients. the histopathological results of cervix were analyzed by colposcopic biopsy, dilatation and curettage (d&c), cervical loop electrosu ...	2011	21600120
a case of multiple squamous cell papillomas of the trachea.	we report a case of 68-year-old woman suffering from breathlessness on exertion with stridor. a chest computed tomography showed a tumor arising from the posterior wall of the trachea. the diagnosis was squamous cell papilloma of the surgically removed tumor, which had caused the asphyxiation. after removal of the tumor, the patient received radical therapy: semiconductor laser transpiration. polymerase chain reaction (pcr) detected human papilloma virus (hpv) type 6, thought to be the cause of ...	2011	21597426
[papillomavirus and head and neck cancer.]	tobacco and alcohol consumption are the main risk factors for head and neck cancers. papillomavirus (hpv) infection was recently associated with the development of malignant tumors of the oropharynx, according to molecular and biological arguments. we describe the oncogenic mechanisms of hpv infections, the epidemiological and clinical aspects of associated head and neck cancers, their prognosis, and issues of specific therapeutic strategies.	2011	21596407
molecular epidemiology of hpv infection using a clinical array methodology in 2952 women in greece.	clin microbiol infect abstract: the molecular epidemiology of human papillomavirus (hpv) infection in a sample of greek women (n = 2952, mean age 42.2 ± 13.3 years) was examined. hpv prevalence was 50.7% (95% confidence interval, 48.8-52.6). the most frequent hpv types were hpv 53, 51 and 66 (10.2%, 9.4% and 9.3%, respectively). hpv positivity was associated with age, age of sexual debut, number of sexual partners and duration of sexual relationship, while marriage or multiparity protected again ...	2011	21595788
green and black tea in relation to gynecologic cancers.	scope: observational studies have evaluated the relationship between green tea intake and cancers of the ovary and endometrium, but we are not aware of the published studies on green tea intake and risk of human papillomavirus (hpv)-related cancers of the cervix, vagina, or vulva. methods and results: a critical review of the published literature on tea intake and risk of ovarian and endometrial cancers was conducted. in meta-analyses, we report inverse associations for green tea intake and risk ...	2011	21595018
ccnd1 rs9344 polymorphisms are associated with the genetic susceptibility to cervical cancer in chinese population.	cyclin d1, with a common g/a polymorphism in rs9344, is an essential regulator of the g1 phase in cell cycles and plays an important role in several tumor types, and the homology of cyclin d1 with human papillomavirus (hpv)-16 e7 brought our attention to ccnd1 gene in cervical cancer. a total of 738 native chinese subjects consist of 327 cases and 411 controls were enrolled in this study. ccnd1 genotyping was analyzed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rfl ...	2011	21594903
differential survival trends for patients with tonsillar, base of tongue and tongue cancer in sweden.	tonsillar, base of tongue and tongue cancer have similar anatomical and histopathological appearances but present differences in prognosis. human papillomavirus (hpv) is a known risk factor for tonsillar and base of tongue cancer, and a survival benefit has been shown for these tumors; however, hpv prevalence in tongue cancer is low. tonsillar, base of tongue and tongue cancer patients registered in the swedish cancer registry between 1960 and 2004 were followed from the date of cancer diagnosis ...	2011	21592846
cytopathic effects of human papillomavirus infection and the severity of cervical intraepithelial neoplasia : a frequency study.	cytopathic effects related to the human papillomavirus (hpv) infection are more frequently found in cervical intraepithelial neoplasia (cin) 1; however, there are indications that at least half the histological diagnoses of cin2 and cin3 include koilocytosis areas. the objective of this study was to evaluate the frequency of the cytological criteria suggestive of hpv infection in the cervical smears of women with a histological diagnosis of cin. one hundred and sixty-two women with abnormal cerv ...	2011	21591275
correlation between human papillomavirus infection and clinicopathological parameters in anal canal carcinoma.	we evaluated human papillomavirus (hpv) dna in 17 anal canal tumors and its correlation with symptomatology, tumor extension and prognosis. five squamous carcinomas and 4 cloacogenic tumors resulted hpv+. statistical analysis showed no correlation between hpv infection and tumor morphology, lymph node involvement or prognosis, and no significant difference in the duration of symptoms between hpv+ and hpv- patients. hpv are involved in the pathogenesis of the tumors, but are not responsible for a ...	1997	21590048
should males be vaccinated against hpv?		2011	21587022
knowledge of pap smear, hpv and the hpv vaccine and the acceptability of the hpv vaccine by thai women.	aims: the primary prevention for cervical cancer, a human papilloma virus (hpv) vaccine, has been available in thailand for almost 3 years. the present study evaluates knowledge about the papanicolaou (pap) smear, hpv and the hpv vaccine and focuses on identifying predictors for the acceptability of the hpv vaccine. methods: a sample of 764 women attending the gynecology clinic at ramathibodi hospital, bangkok, was asked to answer a questionnaire on their personal background, their knowledge o ...	2011	21585696
the impact of cytology screening and hpv vaccination on the burden of cervical cancer.	aim: to evaluate the impact of different strategies of human papillomavirus (hpv) vaccination on the burden of cervical cancer in singapore. methods: the incidence of cervical cancer was calculated using a markov model with inputs based on singapore data for the prevalence of hpv infection, socioeconomic characteristics and screening prevalence. the evaluation was performed for 10 scenarios: no screening, current opportunistic cytology screening, ideal optimal screening, universal adolescent h ...	2011	21585695
cancerous inhibitor of protein phosphatase 2a is overexpressed in cervical cancer and upregulated by human papillomavirus 16 e7 oncoprotein.	objectives: cancerous inhibitor of protein phosphatase 2a (cip2a) is a recently identified oncoprotein stabilizing c-myc and promoting cell proliferation and transformation. here we investigated the role of cip2a in cervical cancer in vivo and in vitro. methods: cip2a expression was assessed in normal cervical, cervical intraepithelial neoplasia (cin) i to iii and cervical cancer tissues by immunohistochemistry and rt-pcr. cell growth was explored by cell proliferation assay, colony formation as ...	2011	21575984
a protein profile study to discriminate cin lesions from normal cervical epithelium.	background: cervical intraepithelial neoplasia (cin), a frequently encountered disease caused by human papilloma virus (hpv) is often diagnosed in formaldehyde-fixed paraffin embedded (ffpe) punch biopsies. since it is known that this procedure strongly affects the water-soluble proteins contained in the cervical tissue we decided to investigate whether a water-soluble protein-saving biopsy processing method can be used to support the diagnosis of normal and cin. methods: cervical punch biopsies ...	2011	21573931
genital human papillomavirus infection in patients with autoimmune inflammatory diseases.	treatment advances achieved over the last few years have radically changed the management of patients with autoimmune inflammatory diseases requiring conventional or biological immunosuppressive therapy. these diseases and the drugs used to treat them increase the rate of infections, including genital infections due to the human papillomavirus (hpv). genital hpv infections have been extensively studied in organ transplant recipients, hiv-infected patients, and patients with congenital immune def ...	2011	21570889
human papillomavirus vaccine introduction in low and middle income countries: guidance on the use of cost-effectiveness models.	abstract: background: the world health organization recommends that the cost-effectiveness of introducing human papillomavirus (hpv) vaccination is considered before such a strategy is implemented. however, developing countries often lack the technical capacity to perform and interpret results of economic appraisals of vaccines. to provide information about the feasibility of using such models in a developing country setting, we evaluated models of hpv vaccination in terms of their capacity, req ...	2011	21569406
human papillomavirus infection in nasal polyps in a chinese population.	in order to determine the prevalence and genotype distribution of human papillomavirus (hpv) infection in patients with nasal polyps, a total of 204 patients with nasal polyps and 36 healthy controls were recruited in this study. genomic deoxyribonucleic acid (dna) was extracted from paraffin-embedded tissue sections. hpv dna genotyping was achieved by a flow-through hybridization and gene chip method. hpv infection was positively identified in 82 of 204 (40.2%) patients, while hpv dna was not f ...	2011	21562117
clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants.	background: isolates of hpv16 comprise six variants: european (eu), asian (as), asian-american (aa), north american (na), african-1 (af1), and african-2 (af2) with different carcinogenic potentials. highly reliable automatable techniques for hpv variant genotyping would be helpful to confirm the role of variants in cervical cancer in large epidemiological studies. objective: to validate the performance of a novel assay for identification of hpv16 variants. study design: the test is a multiplex p ...	2011	21561803
current status of prostate-cancer research - development of in-vitro model systems (review).	prostate cancer is a major medical problem which is expected to affect over 200,000 us men in 1994. despite its widespread prevalence and the difficulties in clinical diagnosis and treatment of the disease, the contribution of environmental factors and the etiological mechanism of prostate cancer are poorly understood. a brief update on recent progress in cellular and molecular research of prostate cancer is provided. specific areas discussed include oncogenes (vas, myc, c-erbb-2 and bcl-2), tum ...	1994	21559703
effect of actinomycin-d on the cell-cycle progression and the expression of p53, waf1/cip1, gadd45, and mdm-2 genes in human oral keratinocytes - implication of human papillomavirus infection.	exposure of human oral keratinocytes immortalized by transfection with 'high risk' hpv dna to chemical carcinogens converts the cells to malignant phenotype, but it does not transform normal cells. to investigate the underlying mechanism for different chemical carcinogen susceptibility of normal and the hpv-immortalized oral keratinocytes to genotoxic agent, we studied the progression of cell cycle and the expression of p53, waf1/cip1, gadd45 and mdm-2 genes in normal and in the hpv-immortalized ...	1994	21559675
prevalence and risk factors of human papillomavirus (hpv) infection in southern chinese women - a population-based study.	persistent high-risk type human papillomavirus (hpv) infection is recognized as a necessary cause of cervical cancer. this study aimed to compare the hpv prevalence and risk factors between women residing in hong kong (hk) and guangzhou (gz) region of china.	2011	21559276
the relationship of knowledge, health value and health self-efficacy with men's intentions to receive the human papillomavirus (hpv) vaccine.	the aim of the present study was to determine whether it was possible to predict human papillomavirus (hpv) vaccination intentions an australian male sample. one hundred and twenty one males aged 18 to 26 (m = 22.3, sd = 2.06), completed an online survey assessing hpv-related knowledge, health beliefs and health self-efficacy. hpv knowledge and health self-efficacy were significant independent predictors of vaccine acceptance. a moderating effect was found between hpv knowledge and health self-e ...	2011	21558104
the gene encoding the p53-regulated inhibitor of cdks (pic1) is not expressed in the molt-4 leukemia-cell line with p53 truncated at the carboxyl-terminus, and harbors a nucleotide substitution at codon-31 in several other cancer cell-lines.	entry into the cell cycle is governed by cyclins, cyclin-dependent kinases (cdks) and cdk-inhibitors (cdkis). the p53-regulated inhibitor of cdks (pic1) is a universal cdki whose gene expression is directly induced by the p53 tumor suppressor protein. reverse transcription and polymerase chain reaction revealed strong pic1 gene expression in control mrc-5 human embryo lung cells, but relatively weaker bands in a549 lung carcinoma; hep3b, mahlavu, plc/prf/5 hepatocellular carcinoma; siha, caski, ...	1995	21556614
polymorphism of the hpv-16 e6 gene of cervical-carcinoma.	polymorphism of the human papillomavirus type 16 (hpv-16) genome has been reported to occur within the noncoding regulatory long control region (lcr) and in the e7 and l1 genes. the current study focuses on the hpv-16 e6 oncogene which interacts with the antioncogenic regulator p53. seventy-eight hpv-16-positive dna samples derived from cervical carcinomas were screened for the presence of polymorphism in the hpv-16 e6 gene by polymerase chain reaction (pcr) linked single stranded conformational ...	1995	21552834
considerations on the clinical application of the human papillomavirus vaccine in spain.	the circumstances that in the opinion of the authors influence the lower than expected uptake of the vaccine against human papillomavirus in spain are analyzed. among others, aspects related to doubts about efficacy and safety, the attitude of the spanish health authorities or the role of media and the antivaccine groups, as well as the vaccination "culture" of the gynecologist, are discussed. the authors conclude that only with the coordinated effort of all involved bodies in the vaccination pr ...	2011	21552002
working to close the gap: identifying predictors of hpv vaccine uptake among young african american women.	abstract:factors associated with greater likelihood of human papillomavirus (hpv) vaccination among women who experience excess cervical cancer incidence and mortality requires further study. the purpose of this study was to assess factors associated with hpv vaccine uptake among young african american women. three hundred sixty-three african american women aged 18-26 were recruited from historically black colleges/universities to complete a questionnaire to assess health beliefs associated with ...	2011	21551933
reported adverse events in girls aged 13-16 years after vaccination with the human papillomavirus (hpv)-16/18 vaccine in the netherlands.	in 2009, human papillomavirus (hpv) vaccination was offered to girls born in 1993-1996 in a catch-up campaign, followed in 2010 by the implementation of the vaccination in the national immunization programme (nip) for girls born in 1997. to monitor the tolerability of the 2009 catch-up campaign, we investigated the occurrence of adverse events within 7 days after vaccination with the bivalent hpv vaccine. a total of 6000 girls were asked to participate, including 1500 from each birth cohort from ...	2011	21549785
cervical human papilloma virus infection among the general female population in vietnam: a situation analysis.	human papilloma virus (hpv) is the necessary cause of cervical cancer. this survey used a sample of 1,500 married women aged 15-69 to examine the prevalence of hpv infection and hpv specific types in vietnam as well as risk factors of hpv infection. results indicated that the prevalence of hpv infection in hanoi and hcm was 6.13 and 8.27. the proportion of multiple hpv infection was also higher in hcm than in hanoi (35.5% vs. 17.4%). risk factors having significant associations with general hpv ...	2011	21545230
[prevalence of high- and low-risk oncogenic human papillomaviruses in patients with external genital pathology].	the paper presents the results of examining 49 patients with genital papillomas, vulvar and vaginal leukoplakia, dysplasia, and cancer. the findings may suggest that human papillomavirus plays an important role in the development of vulvar and vaginal lesions and reconsider the importance of high- and low-risk oncogenic genotypes in the development of benign neoplasms, precancerous conditions, and malignant tumors of the vulva and vagina.	2011	21545037
detection of human papilloma virus (hpv) and k-ras mutations in human lung carcinomas.	the purpose of our study was to assess the prevalence and prognostic significance of hpv infection as well as k-ras codon 12 point mutations in lung cancer. patients diagnosed with lung carcinoma between 1988 and 1992 (n=99) were selected. hpv detection and typing was performed by pcr from paraffin-embedded tissues, while mutations in codon 12 of k-ras gene were detected using the restriction fragment length polymorphism (rflp) analysis. the prevalence of hpv infection was 15%, while k-ras codon ...	1996	21544468
p53 levels in human mammary epithelial cells expressing wild-type and mutant human papillomavirus type 16 (hpv-16) e6 proteins.	human fibroblast cells must overcome both the m1 and the m2 stages of cellular senescence to immortalize, at which point cells almost always express telomerase activity. the human papillomavirus (hpv) oncoproteins, hpv-16 e6 and e7, can block the progression to senescence in fibroblasts by associations with p53 and prb, respectively. human mammary epithelial (hme) cells require only hpv-16 e6 to bypass m1, suggesting that prb may not have a direct role in hme cells senescence. in the present rep ...	1996	21544354
characterisation of cellular changes which influence progression of human papillomavirus type-16 immortalised keratinocytes to anchorage-independent phenotype.	cellular alterations which influence progression to an anchorage-independent phenotype are poorly understood. few immortalised keratinocyte lines have been reported to form colonies in semi-solid medium, and the important role of the anchorage-dependence in controlling the behaviour of keratinocytes reflects the resistance of these cells to form colonies in soft agar. we describe here a model for studying in vitro the progression of human papillomavirus type-16 (hpv-16) immortalised keratinocyte ...	1996	21544345
additive inhibitory effects of retinoids and interferon-alpha on the growth of human cervical carcinoma cells.	the combination of 13-cis retinoic acid (13cra) and interferon-alpha (ifn-alpha) has shown marked antitumor activity against locally advanced cervical cancer in vivo. to begin to explore the mechanism and potential of the efficacy of this combination, the sensitivity of 9 cervical carcinoma cell lines to the growth inhibitory effects of these agents was examined after 1 to 7 days of treatment. ifn-alpha (100 u/ml) alone exerted variable effects. siha cell line was very sensitive showing 75% inhi ...	1996	21544324
nrip, a novel calmodulin binding protein, activates calcineurin to dephosphorylate hpv e2 protein.	previously, we found a gene named nuclear receptor interaction protein (nrip) (or dcaf6 or iqwd1). we demonstrate that nrip is a novel binding protein for human papillomavirus 16 (hpv-16) e2 protein. hpv-16 e2 and nrip can directly associate into a complex in vivo and in vitro, and the n-terminal domain of nrip interacts with the transactivation domain of hpv-16 e2. only full-length nrip can stabilize e2 protein and induce hpv gene expression, and nrip silenced by two designed sirnas decreases e ...	2011	21543494
polymorphisms of mdm4 and risk of squamous cell carcinoma of the head and neck.	purpose: mouse double minute 4 (mdm4), a homolog of mdm2, is one of the key negative regulators of p53, and its amplification or overexpression contributes to carcinogenesis by inhibiting the p53 tumor suppressor activity. we investigated the association between mdm4 polymorphisms and the risk of squamous cell carcinoma of the head and neck (scchn). methods: we genotyped three mdm4 tagging polymorphisms, two in the 3' untranslated region (rs11801299g>a and rs10900598g>t) and one in intron 1 (rs1 ...	2011	21540763
long-term impact of human papillomavirus vaccination on infection rates, cervical abnormalities, and cancer incidence.	: vaccination against human papillomavirus (hpv) types 16/18 is being implemented in many countries. there may be indirect benefit of hpv vaccination to nonvaccinated women, who may experience a reduced risk of infection with vaccine-preventable types (herd immunity). we attempt to disentangle the direct and indirect effects of hpv vaccination, while accounting for 14 oncogenic hpv types in a dynamic modeling framework.	2011	21540743
completion and timing of the three-dose human papillomavirus vaccine series among adolescents attending school-based health centers in oregon.	many adolescents do not complete the 3-dose human papillomavirus vaccine series in the recommended time frame, or at all. given the challenges of administering a multi-dose vaccine to adolescents, especially those in vulnerable populations, we evaluated completion of the human papillomavirus vaccine series in 19 of oregon's school-based health centers.	2011	21539853
viruses and the fuel sensor: the emerging link between ampk and virus replication.	adenosine 5' monophosphate-activated protein kinase (ampk) is conserved in all eukaryotic cells and functions as the key regulator of cellular metabolism by responding to the energy status of the cell. it is activated by an increase in the amp:atp ratio and then attempts to redress the balance by upregulating catabolic processes, whilst concomitantly inhibiting anabolic processes. despite its critical importance in the functioning of eukaryotic cells, there has been a paucity of studies investig ...	2011	21538667
the potential role of self-sampling for high-risk human papillomavirus detection in cervical cancer screening.	high-risk human papillomavirus (hr-hpv) detection will become an important tool in the screening for cervical cancer. self-sampling is an inexpensive and well-accepted method for hpv detection that will increase participation of nonresponders in current screening programs. even more, because self-collected samples are as good as physician-collected samples for hpv detection, self-sampling might be a suitable method for future primary cervical cancer screening. copyright © 2011 john wiley & sons, ...	2011	21538664
regulation of nucleocytoplasmic trafficking of viral proteins: an integral role in pathogenesis?	signal-dependent targeting of proteins into and out of the nucleus is mediated by members of the importin (imp) family of transport receptors, which recognise targeting signals within a cargo protein and mediate passage through the nuclear envelope-embedded nuclear pore complexes. regulation of this process is paramount to processes such as cell division and differentiation, but is also critically important for viral replication and pathogenesis; phosphorylation appears to play a major role in r ...	2011	21530593
nucleotide deficiency promotes genomic instability in early stages of cancer development.	chromosomal instability in early cancer stages is caused by stress on dna replication. the molecular basis for replication perturbation in this context is currently unknown. we studied the replication dynamics in cells in which a regulator of s phase entry and cell proliferation, the rb-e2f pathway, is aberrantly activated. aberrant activation of this pathway by hpv-16 e6/e7 or cyclin e oncogenes significantly decreased the cellular nucleotide levels in the newly transformed cells. exogenously s ...	2011	21529715