Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| toxoplasma gondii and neospora caninum in sparrows (passer domesticus) in the northeast of brazil. | toxoplasma gondii is a cosmopolitan protozoan parasite of warm-blooded animals that causes high rates of infection in mammals and birds. sparrows (passer domesticus) are synantropic birds which are distributed worldwide. they serve as intermediate hosts for the parasite but are quite resistant to toxoplasmosis. the aims of this study were to determine the frequency of t. gondii infection in sparrows using serologic and molecular tests, and to investigate related parasites, such as neospora canin ... | 2010 | 19879051 |
| primary culture of intestinal epithelial cells as a potential model for toxoplasma gondii enteric cycle studies. | the primary culture of intestinal epithelial cells from domestic cats is an efficient cellular model to study the enteric cycle of toxoplasma gondii in a definitive host. the parasite-host cell ratio can be pointed out as a decisive factor that determines the intracellular fate of bradyzoites forms. the development of the syncytial-like forms of t. gondii was observed using the 1:20 bradyzoite-host cell ratio, resulting in similar forms described in in vivo systems. this alternative study potent ... | 2009 | 19876557 |
| cervical lymphadenitis in a patient coinfected with toxoplasma gondii and bartonella henselae. | cat scratch disease, caused by bartonella henselae, is a worldwide zoonosis that is most frequently associated with the bite or scratch of a kitten under 6 months of age, as well as from a fleabite. toxoplsma gondii is also another important zoonotic agent in cats and humans, which is mainly acquired by ingestion of food or water that is contaminated with oocytes shed by cats or by eating undercooked or raw meat containing tissue cysts. here, we report a first case of young patient with cervical ... | 2010 | 19874186 |
| toxoplasma and schizophrenia. | research on infectious agents as a possible cause of schizophrenia has become prominent in the past decade. toxoplasma gondii has emerged as a prime candidate for a variety of reasons; (i) many studies have reported that individuals with schizophrenia, compared to controls, have a higher prevalence of antibodies to t. gondii, (ii) some individuals with adult toxoplasmosis develop psychotic symptoms similar to those of schizophrenia, (iii) epidemiologically, there are many similarities between to ... | 2009 | 19825110 |
| cross-sectional survey of toxoplasma gondii infection in colony cats from urban florence (italy). | cats are the key species in the epidemiology of toxoplasma gondii infection, even if the proportion of subjects excreting oocysts is low. the aim of the present paper was to obtain information about seroprevalence, oocyst shedding rate and presence of t gondii dna in faeces collected from an urban population of colony cats in florence (tuscany). fifty european shorthair feral cats were examined for anti-t gondii specific antibodies by a modified agglutination test (mat), and for oocysts by micro ... | 2010 | 19811938 |
| toxoplasmosis among pregnant women in qualyobia governorate, egypt. | this study was conducted between august 2007 and october 2008 to identify the sero-prevalence of toxoplasma gondii among pregnant women of different ages and stages of gestation. latex agglutination test was used as screening test. elisa-igg & igm tests measured for the toxo-latex positive cases to identify toxoplasmosis clinical status. the results indicated that the prevalence of t. gondii among pregnant women was relatively high in the rural (57.6%) than urban (46.5%) areas. the positivity wa ... | 2009 | 19795746 |
| [occurrence of antibodies and risk factors for infection for toxoplasma gondii in dogs in the cities of lages and balneário camboriú, santa catarina state, brazil]. | the aim of this study was to identify the occurrence of antibodies and risk factors for infection for toxoplasma gondii in dogs in the cities of lages and balneário camboriú, santa catarina state, brazil. blood samples from 400 domiciliated dogs were processed for antibody detection against t. gondii by indirect immunofuorescence antibody test (ifat). the following variables were obtained by questionnaire: breed, age, sex, diet, environment, presence of cats and street access. a chi-square test ... | 2009 | 19772776 |
| evidence for high levels of vertical transmission in toxoplasma gondii. | toxoplasma gondii is a highly ubiquitous and prevalent parasite. despite the cat being the only definitive host, it is found in almost all geographical areas and warm blooded animals. three routes of transmission are recognised: ingestion of oocysts shed by the cat, carnivory and congenital transmission. in natural populations, it is difficult to establish the relative importance of these routes. this paper reviews recent work in our laboratory which suggests that congenital transmission may be ... | 2009 | 19765335 |
| cats and toxoplasma: implications for public health. | cats are popular as pets worldwide because they are easy to care for and provide companionship that enriches the lives of human beings. little attention has been focused on their potential to contaminate the environment with zoonotic pathogens. one such pathogen, the protozoan parasite toxoplasma gondii, rarely causes clinical manifestations in cats or immunocompetent humans; however, it can have serious adverse effects on human foetuses and immunocompromised patients. many human infections are ... | 2010 | 19744306 |
| toxoplasma gondii infections in chickens (gallus domesticus): prevalence, clinical disease, diagnosis and public health significance. | chickens are considered one of the most important hosts in the epidemiology of toxoplasma gondii infection because they are an efficient source of infection for cats that excrete the environmentally resistant oocysts and because humans may become infected with this parasite after eating undercooked infected chicken meat. the objective of this study is to review worldwide prevalence of t. gondii infection in chickens and to assess the role of infected chickens in the epidemiology of toxoplasmosis ... | 2010 | 19744305 |
| a brief history and overview of toxoplasma gondii. | toxoplasma gondii was discovered by scientists working in north africa and brazil around 100 years ago. the parasite has since been found to be capable of infecting all warm-blooded animals including humans making it one of the most successful parasitic organisms worldwide. the pathogenic potential of t. gondii was recognized in the 1920s and 1930s, in congenitally infected children presenting with the classic triad of symptoms, namely hydrocephalus, retinochoroiditis and encephalitis. in additi ... | 2010 | 19744303 |
| toxoplasmosis prevention and testing in pregnancy, survey of obstetrician-gynaecologists. | toxoplasmosis in pregnant women can lead to congenital disease with severe neurological and ocular complications in the foetus. in 2006, we surveyed us obstetrician-gynaecologists to determine their knowledge and practices about toxoplasmosis prevention and testing. questionnaires were mailed (four mailings) to a random sample of 1200 of the 33,354 members of the american college of obstetricians and gynecologists (acog). of the 1200 surveyed, 502 (42%) responded. the respondents were similar to ... | 2010 | 19744302 |
| infection with toxoplasma gondii during pregnancy: seroepidemiological studies in austria. | seropositivity among pregnant woman in austria has decreased from 48% to 50% at the end of the 1970s to 35% in recent years. despite this decrease, knowledge of possible sources and risk factors for toxoplasma infection remains important. we reviewed seroepidemiological studies that were undertaken to assess the roles of undercooked meat and oocysts in cat faeces as potential sources of infection in pregnant women. improved management and hygiene in pig herds raised in confinement have resulted ... | 2010 | 19744300 |
| toxoplasma gondii: prototype immunization of lambs against formation of muscle and brain cysts. | nine lambs were immunized with 10(6) oocysts of the relatively avirulent me-49 strain of toxoplasma gondii, and were challenged 45 days later with 4 x 10(6) oocysts of the m3 strain of this parasite. less than 5 tissue cysts of the m3 strain formed per 8 g of muscle - if any - judging from a bioassay in mice in which all mice survived. in contrast, mortality occurred in mice fed muscle specimens from 4 lambs that had not been immunized with me-49 prior to challenge with the m3 strain. this findi ... | 2009 | 19729248 |
| infectivity of toxoplasma gondii in northern traditional (country) foods prepared with meat from experimentally infected seals. | serological and clinical evidence of human toxoplasmosis in the canadian arctic indicates a food safety risk associated with the consumption of wild game meat. such meat often is eaten raw or partially cooked in locally prepared traditional (country) foods, but no data have been collected to describe survival of toxoplasma gondii forms in these foods. the muscle of grey seals (halichoerus grypus) experimentally infected with t. gondii oocysts was used to prepare three country foods: igunaq, a fe ... | 2009 | 19722415 |
| atypical toxoplasma gondii genotypes identified in oocysts shed by cats in germany. | a total of 18,259 feline faecal samples from cats in germany were collected and analysed for the presence of toxoplasma gondii oocysts between june 2007 and december 2008. the proportion of t. gondii-positive samples collected between january and june was significantly lower than between july and december. the age of cats shedding t. gondii oocysts was not significantly different from the age of negative control cats. forty-six t. gondii-positive samples were genetically characterised using nine ... | 2010 | 19695254 |
| genetic diversity of toxoplasma gondii in animals and humans. | toxoplasma gondii is one of the most widespread parasites of domestic, wild, and companion animals, and it also commonly infects humans. toxoplasma gondii has a complex life cycle. sexual development occurs only in the cat gut, while asexual replication occurs in many vertebrate hosts. these features combine to create an unusual population structure. the vast majority of strains in north america and europe fall into three recently derived, clonal lineages known as types i, ii and iii. recent stu ... | 2009 | 19687043 |
| seroprevalence of toxoplasma gondii and neospora caninum in feral cats (felis silvestris catus) in majorca, balearic islands, spain. | felids are important in the epidemiology of toxoplasma gondii infection because they are the only hosts that can excrete environmentally resistant oocysts. antibodies to t. gondii and neospora caninum were determined in serum samples from 59 feral cats (felis silvestris catus) captured in baited traps during authorized predator control campaigns in majorca, balearic islands, spain. antibodies to t. gondii assayed by the modified agglutination test (mat) were found in 50 (84.7%) of 59 cats with m ... | 2009 | 19665309 |
| risk factors for toxoplasma gondii infection in the united states. | toxoplasmosis can cause severe ocular and neurological disease. we sought to determine risk factors for toxoplasma gondii infection in the united states. | 2009 | 19663709 |
| toxoplasma gondii: 25 years and 25 major advances for the field. | this article is an attempt to identify the most significant highlights of toxoplasma research over the last 25 years. it has been a period of enormous progress and the top 25 most significant advances, in the view of this author, are described. these range from the bench to the bedside and represent a tremendous body of work from countless investigators. and, having laid out so much that has been discovered, it is impossible not to also reflect on the challenges that lie ahead. these, too, are b ... | 2009 | 19630140 |
| history of the discovery of the life cycle of toxoplasma gondii. | it has been 100 years since the discovery of toxoplasma gondii in 1908. its full life cycle was not discovered until 1970 when it was found that it is a coccidian parasite of cats with all non-feline warm blooded animals (including humans) as intermediate hosts. the discovery of the environmentally resistant stage of the parasite, the oocyst, made it possible to explain its worldwide prevalence. in the present paper, events associated with the discovery of its life cycle are recalled. | 2009 | 19630138 |
| experimental infection of pregnant queens with two major brazilian clonal lineages of toxoplasma gondii. | toxoplasma gondii isolates from brazil are biologically and genetically different from european and north america isolates. recently, four genotypes were considered the common clonal lineages in brazil and were designated as types bri, brii, briii, and briv. the pathogenicity of two major brazilian lineages was investigated after oral inoculation of queens in the middle third of their pregnancies with t. gondii cysts. twelve pregnant queens without t. gondii antibodies were distributed in group ... | 2009 | 19629528 |
| identification of faecal transmission of toxoplasma gondii: small science, large characters. | the first clue to the elucidation of the complete life cycle of toxoplasma gondii was the identification of an infectious form in cat faeces that could be transmitted orally and could survive in the external environment for extended periods. this personal review describes the scientist (w.m. hutchison) and the background to the initial discovery and covers the period to the complete elucidation of the life cycle of t. gondii. | 2009 | 19596630 |
| seroprevalence of and risk factors for toxoplasma gondii in sheep raised in the jaboticabal microregion, são paulo state, brazil. | seroprevalence of and risk factors for toxoplasmosis in sheep from different properties in the jaboticabal microregion, são paulo state, brazil were determined. antibodies to toxoplasma gondii were found in sera of 52.0% of 488 sheep tested by indirect fluorescent antibody test (ifat > or =64). t. gondii seropositivity in sheep was significantly associated with gender of the sheep, pasturing system, contact with cats, and the use of mineral supplements and the type of feed. | 2010 | 19589550 |
| genetic characterization of toxoplasma gondii isolates from china. | toxoplasma gondii infections are prevalent in humans and animals worldwide. in north america and europe, t. gondii is highly clonal, consisting of three distinct lineages (types i, ii and iii), whereas in south america, t. gondii is highly diverse with a few lineages expanded in the population. however, there is limited data on the diversity of t. gondii in asia. here we report the genetic characterization of t. gondii isolates from different hosts and geographical locations in china using the m ... | 2009 | 19567233 |
| serum feline pancreatic lipase immunoreactivity concentration and seroprevalences of antibodies against toxoplasma gondii and bartonella species in client-owned cats. | feline pancreatitis is a commonly suspected illness and it has been proposed that some cases of feline pancreatitis may be caused by infection with toxoplasma gondii or bartonella species. feline pancreatic lipase immunoreactivity (fpli) is a test performed on serum that is commonly combined with other clinical findings as an indirect aid in the diagnosis of pancreatitis. the purpose of this study was to determine if there are associations between fpli concentration and the presence of serum ant ... | 2009 | 19560385 |
| isolation of toxoplasma gondii from the brain of a dog in australia and its biological and molecular characterization. | toxoplasma gondii was isolated from the brain of a young dog for the first time in australia. the identity of the parasite was confirmed by pcr, western blotting, electron microscopy and cat bioassay. genotyping of the isolate (tgdgau1) was determined by pcr-rflp markers that showed it to be a type ii strain. western blotting demonstrated the presence of igm antibodies to t. gondii suggesting the bitch was probably infected during pregnancy and the t. gondii was transmitted to the pups congenita ... | 2009 | 19556061 |
| sarcocystis sp. encephalomyelitis in a cat. | a 5-month-old male neutered domestic shorthair cat was evaluated for spinal pain, ataxia, and anisocoria. neuroanatomic localization indicated diffuse or multifocal central nervous system disease. on cerebrospinal fluid analysis, neutrophilic pleocytosis and intracellular protozoal merozoites were observed. the merozoites were oval, 2-4 microm in width and 4-6 microm in length, and had linear arrays of nuclear material concentrated at one pole. serum was positive for sarcocystis sp. antibodies a ... | 2010 | 19548967 |
| the ferredoxin-nadp+ reductase/ferredoxin electron transfer system of plasmodium falciparum. | in the apicoplast of apicomplexan parasites, plastidic-type ferredoxin and ferredoxin-nadp(+) reductase (fnr) form a short electron transport chain that provides reducing power for the synthesis of isoprenoid precursors. these proteins are attractive targets for the development of novel drugs against diseases such as malaria, toxoplasmosis, and coccidiosis. we have obtained ferredoxin and fnr of both toxoplasma gondii and plasmodium falciparum in recombinant form, and recently we solved the crys ... | 2009 | 19523113 |
| seroprevalence of human infection with toxoplasma gondii and the associated risk factors, in maiduguri, borno state, nigeria. | the prevalence of antibodies (igg) to toxoplasma gondii among 180 adults from the nigerian city of maiduguri was determined as 23.9%, using a commercial elisa. although the men investigated were more likely to be seropositive than the women, the difference was not statistically significant (27.3% v. 19.8%; p>0.05). there was also no apparent association between educational level and seroprevalence. there was a positive correlation between the mean antibody titre and the age of the subjects, with ... | 2009 | 19508749 |
| review of idiopathic eosinophilic meningitis in dogs and cats, with a detailed description of two recent cases in dogs. | eosinophilic meningoencephalitis (eme) has been described in various species of animals and in humans. in dogs it has been associated with protozoal infections, cuterebral myiasis and various other aetiologies. ten cases of idiopathic eosinophilic meningoencephalitis have been reported in dogs and one in a cat where the origin was uncertain or unknown. the dogs were all males, of various breeds but with a predominance of golden retrievers and rottweilers; they generally had a young age of onset. ... | 2008 | 19496320 |
| toxoplasma seroprevalence in a rural population in france: detection of a household effect. | toxoplasma gondii, the agent of toxoplasmosis, has a complex life cycle. in humans, the parasite may be acquired either through ingestion of contaminated meat or through oocysts present in the environment. the importance of each source of contamination varies locally according to the environment characteristics and to differences concerning human eating habits and the presence of cats; thus, the risk factors may be determined through fine-scale studies. here, we searched for factors associated w ... | 2009 | 19476609 |
| prevalence and risk factors associated to infection by toxoplasma gondii in ovine in the state of alagoas, brazil. | the aim of this study was to evaluate the prevalence of anti-toxoplasma gondii antibodies and to identify risk factors associated to the infection in the three meso-regions of the state of alagoas, brazil. a total count of 23 towns and 27 meat sheep farms were visited where blood samples were collected in order to perform the indirect immunofluorescence test to evaluate the antibodies presence. questionnaires exploring the production system and nutritional, sanitary, and reproduction handling we ... | 2009 | 19468755 |
| factors associated with seropositivity for anti-toxoplasma gondii antibodies in pregnant women of londrina, paraná, brazil. | the aim of this study was to evaluate associations between seropositivity for igg and igm anti-toxoplasma gondii antibodies and socio-economic and environmental variables in pregnant women of londrina, state of paraná, brazil. we interviewed 492 pregnant women, each of whom answered an epidemiological questionnaire, and collected blood samples for measurement of igg and igm anti-t. gondii antibodies by chemiluminescence. a confirmatory diagnosis of acute infection was made by an igg avidity test ... | 2009 | 19430668 |
| veterinary vaccines against toxoplasma gondii. | toxoplasma gondii has a very wide intermediate host range and is thought to be able to infect all warm blooded animals. the parasite causes a spectrum of different diseases and clinical symptoms within the intermediate hosts and following infection most animals develop adaptive humoral and cell-mediated immune responses. the development of protective immunity to t. gondii following natural infection in many host species has led researchers to look at vaccination as a strategy to control disease, ... | 2009 | 19430650 |
| brazilian contribution for a better knowledge on the biology of toxoplasma gondii. | historically, scientists in brazil has significantly contributed to the biology, cultivation and structural organization of the pathogenic protozoan toxoplasma gondii and its interaction with host cells, starting with the description of the protozoan by splendore in 1908. the intracellular and extracellular corpuscoli observed in rabbits, corresponded to what we now as tachyzoites. later on, a pioneering method to grow t. gondii in tissue cultures was developed by guimarães and meyer, 1942. they ... | 2009 | 19430636 |
| polyganglioradiculoneuritis in a young cat: clinical and histopathological findings. | an 18-month-old european shorthair cat was presented with a two week history of progressive decrease in consciousness, ambulatory tetraparesis, moderate ataxia and generalised decreased-to-absent postural reactions. bilateral facial and nasal hypalgesia, absent menace response and anisocoria were found, and segmental spinal reflexes were normal. neurological signs progressed to nonambulatory tetraparesis, tremor and spinal hyperalgesia. histopathological examination revealed a mild-to-moderate l ... | 2009 | 19425174 |
| isolation and characterization of viable toxoplasma gondii isolates revealed possible high frequency of mixed infection in feral cats ( felis domesticus) from st kitts, west indies. | cats are essential in the epidemiology of toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts in nature. samples of serum, feces, and tissues from feral cats from st kitts, west indies were examined for t. gondii infection. antibodies to t. gondii were assayed by the modified agglutination test, and found in 71 of 96 (73.9%) of cats with titres of 1:10 in six, 1: 20 in six,1:40 in seven,1: 80 in three, 1: 160 in 10, 1:320 in 13, 1:640 in nine, ... | 2009 | 19402949 |
| seroprevalence of toxoplasma gondii and concurrent bartonella spp., feline immunodeficiency virus, and feline leukemia virus infections in cats from grenada, west indies. | toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. feline immunodeficiency virus (fiv) and feline leukemia virus (felv) are related to human immunodeficiency virus, and human leukemia virus, respectively; all of these viruses are immunosuppressive. in the present study, the prevalence of antibodies to t. gondi, bartonella spp., fiv, as well as felv antigen were determined in sera from 75 domestic and 101 feral cats (felis catus) from the caribbean island of grenada, west indie ... | 2009 | 19385716 |
| seroprevalence of toxoplasma gondii in nazaret town, ethiopia. | to determine the seroprevalence of toxoplasmosis, assess its zoonotic importance and identify factors associated with seroprevalence. | 2008 | 19374326 |
| the evolution of the knowledge of cat and dog coccidia. | before the discovery of toxoplasma gondii as a coccidium of the cat in 1970, cat and dog coccidia were classified in the genus isospora and considered of little clinical or zoonotic significance. since 1970, several new (hammondia sp., neospora sp.) and previously described species, including sarcocystis, besnoitia, and cryptosporidium have been found as coccidians of cats and dogs with clinical and zoonotic significance. in the present paper i review salient features of the evolution of cat and ... | 2009 | 19366482 |
| waterborne toxoplasmosis--recent developments. | humans become infected with toxoplasma gondii mainly by ingesting uncooked meat containing viable tissue cysts or by ingesting food or water contaminated with oocysts from the feces of infected cats. circumstantial evidence suggests that oocyst-induced infections in humans are clinically more severe than tissue cyst-acquired infections. until recently, waterborne transmission of t. gondii was considered uncommon, but a large human outbreak linked to contamination of a municipal water reservoir i ... | 2010 | 19324041 |
| coinfection of leptospira spp and toxoplasma gondii among stray dogs in bangkok, thailand. | leptospirosis and toxoplasmosis are zoonotic diseases with global importance. asymptomatic animals harboring these pathogens may act as carriers to other animals including humans. the objective of this study was to investigate the seroprevalence of leptospira and toxoplasma infections in stray dogs in bangkok. a total of 230 stray dogs from monasteries in a bangkok district were examined for specific antibodies to t. gondii and leptospira. the seroprevalence of t. gondii was determined by a modi ... | 2009 | 19323009 |
| seroprevalence of toxoplasma gondii antibodies in pigs, goats, cattle, dogs and cats in peninsular malaysia. | antibodies to the protozoan parasite, toxoplasma gondii were assayed in sera of 200 goats, 100 pigs, 126 cattle from various states of malaysia, and 135 dogs and 55 cats around ipoh region using an indirect fluorescence antibody test (ifat, cut-off titer 1:200); antibodies were found in 35.5% of goats, 14.5% cats, 9.6% dogs, 7.9% local cattle and 4% yellow cattle but not in pigs. results indicate that infection is most prevalent in goats. | 2008 | 19287367 |
| development of forward genetics in toxoplasma gondii. | the development of forward genetics as a functional system in toxoplasma gondii spanned more than three decades from the mid-1970s until now. the initial demonstration of experimental genetics relied on chemically induced drug-resistant mutants that were crossed by co-infecting cats, collecting oocysts, sporulating and hatching progeny in vitro. to capitalise on this, genetic markers were employed to develop linkage maps by tracking inheritance through experimental crosses. in all, three generat ... | 2009 | 19254720 |
| the early years of toxoplasma research: what's past is prologue. | in the century since the first description of toxoplasma gondii history and circumstance have led scientists to define this organism in diverse contexts. from its discovery by researchers shaped by early 20th century versions of the germ theory to its more recent roles as an important globally distributed pathogen and a model apicomplexan, our definitions of toxoplasma are as much a reflection of our frame of reference as they are an absolute definition of this organism. although these transform ... | 2009 | 19250939 |
| risk factors for toxoplasmosis in pigs bred in sicily, southern italy. | the aim of the study was to determine the seroprevalence of toxoplasma gondii infection in pigs raised and slaughtered in sicily, southern italy, and to evaluate the risk factors associated with the infection. samples were collected in seven slaughterhouses and on-site on 274 raising farms across sicily, in the period from january 2006 until march 2007. for each sampled pig born and raised in sicily, information was obtained on gender, age, origin, final destination of meat and farm management. ... | 2009 | 19246158 |
| toxoplasmosis in captive dolphins (tursiops truncatus) and walrus (odobenus rosmarus). | toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment and coastal waters with oocysts. toxoplasma gondii infection was detected in captive marine mammals at a sea aquarium in canada. antibodies to t. gondii were found in all 7 bottlenose dolphins (tursiops truncatus) tested. two of these dolphins, as well as a walrus (odobenus rosmarus) at the facility, died. encephalitis and t. gondii tissue cysts were identified in histological sec ... | 2009 | 19245284 |
| isolate designation and characterization of toxoplasma gondii isolates from pigs in the united states. | pigs are considered to be the most important meat source of toxoplasma gondii for humans in the united states. in the present study, 168 t. gondii isolates (designated tgpgus15-182) from various sources were genotyped using 10 polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) markers (sag1, sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and apico). genotyping data from an additional 14 isolates collected from t. gondii-infected pigs in maryland were included for ana ... | 2009 | 19245283 |
| an outbreak of toxoplasmosis in an aviary collection of nicobar pigeons (caloenas nicobaria). | three out of 10 nicobar pigeons (caloenas nicobaria) in an aviary collection in south africa were found dead with no presenting clinical symptoms. histological examination of formalin-fixed tissues from all these birds revealed necrotic foci in various visceral organs (liver, spleen, heart, kidney and lungs), plus diffuse pulmonary congestion and oedema with vasculitis. numerous protozoal tachyzoites were present in all organs and there was strong positive immunohistochemical (ihc) labelling of ... | 2008 | 19244825 |
| seroprevalence of toxoplasma gondii infection in stray and household cats in guangzhou, china. | the prevalence of anti-toxoplasma gondii specific igg in stray and household cats in guangzhou, china was determined by elisa on serum samples from 206 cats (117 strays and 89 households) and the overall infection rate was 25.24%. the infection rate in stray cats (30.77%) was significantly higher (p < 0.05) than in household cats (17.98%). the rate of infection between male and female cats of both groups was not significantly different (p > or = 0.05), 28.13% versus 32.61% for male and female in ... | 2009 | 19243564 |
| comparison of indirect fluorescent antibody test and modified agglutination test for detecting toxoplasma gondii immunoglobulin g antibodies in dog and cat. | the present study describes the comparison between a modified agglutination test (mat) and the indirect fluorescent antibody test (ifat) for the detection of toxoplasma specific igg antibodies in dog and cat sera. mat showed an "almost perfect" agreement with ifat in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. differences relative to sample dilution were recorded and serological titres obtain ... | 2009 | 19221795 |
| sexual recombination punctuated by outbreaks and clonal expansions predicts toxoplasma gondii population genetics. | the cosmopolitan parasitic pathogen toxoplasma gondii is capable of infecting essentially any warm-blooded vertebrate worldwide, including most birds and mammals, and establishes chronic infections in one-third of the globe's human population. the success of this highly prevalent zoonosis is largely the result of its ability to propagate both sexually and clonally. frequent genetic exchanges via sexual recombination among extant parasite lineages that mix in the definitive felid host produces ne ... | 2009 | 19217909 |
| toxoplasma gondii: congenital transmission in a hamster model. | the objective of the research was to test the hamster for a model of transmission of congenital toxoplasmosis. a non-invasive method for the diagnosis of pregnancy in hamsters was designed, with a specificity and a sensitivity of 70.2 and 94.7%, respectively (n=168). of 32 females with a chronic toxoplasma infection, 3 transmitted toxoplasma congenitally during their first pregnancy, but not during the subsequent pregnancy. congenital transmission rates of infections initiated during pregnancy w ... | 2009 | 19217907 |
| neuropsychiatric disease and toxoplasma gondii infection. | toxoplasma gondii infects approximately 30% of the world's population, but causes overt clinical symptoms in only a small proportion of people. in recent years, the ability of the parasite to manipulate the behaviour of infected mice and rats and alter personality attributes of humans has been reported. furthermore, a number of studies have now suggested t. gondii infection as a risk factor for the development of schizophrenia and depression in humans. as t. gondii forms cysts that are located i ... | 2009 | 19212132 |
| landscape, herd management and within-herd seroprevalence of toxoplasma gondii in beef cattle herds from champagne-ardenne, france. | toxoplasmosis is the most common parasitic zoonosis worldwide. its prevalence and impact on human health are highly variable geographically. humans may be infected by ingesting oocysts from the environment, or bradyzoits contained in meat products from various domestic species, thus data on the dynamics of toxoplasmosis in domestic herds is needed. however, few information is available on the factors that determine the level of infection of cattle herds. in this study, we aimed to estimate withi ... | 2009 | 19155137 |
| rodents as shared indicators for zoonotic parasites of carnivores in urban environments. | rodents are shared intermediate or paratenic hosts for echinococcus multilocularis, toxocara spp. and toxoplasma gondii, and may serve as valuable indicators for assessing the occurrence and the level of environmental contamination and infection pressure with free-living stages of these zoonotic parasites. we investigated 658 non-commensal rodents for parasite infections in the canton of geneva, switzerland. the prevalence of infection with e. multilocularis was highest in arvicola terrestris ca ... | 2009 | 19154652 |
| the history of toxoplasma gondii--the first 100 years. | in this paper the history of toxoplasma gondii and toxoplasmosis is reviewed. this protozoan parasite was first discovered in 1908 and named a year later. its medical importance remained unknown until 1939 when t. gondii was identified in tissues of a congenitally infected infant, and veterinary importance became known when it was found to cause abortion storms in sheep in 1957. the discovery of a t. gondii specific antibody test, sabin-feldman dye test in 1948 led to the recognition that t. gon ... | 2008 | 19120791 |
| prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in sheep from mossoró, rio grande do norte, brazil. | toxoplasma gondii is an obligate intracellular parasite with a variety of hosts, responsible for reproductive problems and economic losses in sheep flocks. neospora caninum was recently identified and its clinical presentation in sheep is similar to that of toxoplasmosis, which can cause repeated abortions, though less frequently in this species. in order to confirm the prevalence of these agents in the city of mossoró, rio grande do norte, brazil, 409 serum samples from adult sheep (364 females ... | 2009 | 19091473 |
| isolation of toxoplasma gondii from horse meat in egypt. | portions of heart, liver, skeletal and diaphragmatic muscles obtained from 150 slaughtered horses at giza-zoo abattoir were used for bioassays in mice and cats. t. gondii tachyzoites were isolated successfully from the peritoneal exudates of the inoculated mice 6-8 days post inoculation with pooled horse tissues. whereas, t. gondii tissue cysts containing bradyzoites were detected in the impression smears of mice brain on the 45th days or more post infection. the oocysts were detected in feces o ... | 2007 | 19070010 |
| seroprevalence of toxoplasma gondii and bartonella spp. antibodies in cats from pennsylvania. | toxoplasma gondii and bartonella spp. are zoonotic pathogens of cats. in the present study, the prevalence of antibodies to t. gondii and bartonella spp. were determined in sera from 210 domestic cats from bensalem, pennsylvania. using a modified agglutination test, antibodies to t. gondii were found in 41 (19.5%) of the 210 cats with titers of 1:25 in 4, 1:50 in 9, 1:100 in 7, 1:200 in 3, 1:400 in 3, 1:800 in 5, 1:1,600 in 4, and 1:3,200 or higher in 6. for t. gondii, seroprevalence increased w ... | 2009 | 19061304 |
| [toxoplasma gondii, an unusual organism with the human as "intermediate host"]. | 2008 | 19058716 | |
| erythrophagocytic low-grade extranodal t-cell lymphoma in a cat. | a 13-year-old male castrated domestic shorthair cat was presented to the referring veterinarian with a 2-month history of weight loss and lethargy. splenomegaly, hepatomegaly, nonregenerative anemia, neutropenia, and hyperbilirubinemia were noted. results of testing for feline immunodeficiency virus, feline leukemia virus, toxoplasma gondii, and mycoplasma sp. were negative. on cytologic examination of aspirates from the enlarged spleen and liver, a population of erythrophagocytic round cells wa ... | 2008 | 19055577 |
| toxoplasmosis in bennett's wallabies (macropus rufogriseus) in spain. | toxoplasmosis is one of the more common parasitic zoonoses world-wide. in this study, an epizootic of toxoplasmosis among captive bennett's wallabies (macropus rufogriseus) from different locations is reported. by means of light microscopy, toxoplasma gondii-like tachyzoites were observed associated to interstitial pneumonia, non-suppurative myocarditis, cholangiohepatitis and severe gastroenteritis. the protozoa stained positively with a t. gondii antibody and ultrastructurally were similar to ... | 2009 | 19046816 |
| detection of toxoplasma gondii in cats by comparing bioassay in mice and polymerase chain reaction (pcr). | feline toxoplasmosis can usually be diagnosed by serological and coprological methods. however, when the interpretation of results is difficult, it becomes necessary to rely on the direct detection of the parasite. in this study, samples of brain from 64 toxoplasma-seropositive cats were subjected to direct detection of toxoplasma gondii by bioassay in mice and nested-pcr. t. gondii was isolated by bioassay in mice in 41 (64%) cases, and nested-pcr was positive in 37 (57.8%) cases (p>0.05). the ... | 2009 | 19038500 |
| toxoplasma gondii: uptake and survival of oocysts in free-living amoebae. | waterborne transmission of the oocyst stage of toxoplasma gondii can cause outbreaks of clinical toxoplasmosis in humans and infection of marine mammals. in water-related environments and soil, free-living amoebae are considered potential carriers of various pathogens, but knowledge on interactions with parasitic protozoa remains elusive. in the present study, we assessed whether the free-living acanthamoeba castellanii, due to its phagocytic activity, can interact with t. gondii oocysts. we rep ... | 2009 | 18992742 |
| toxoplasma-safe meat: close to reality? | in 2008, the centennial of the discovery of toxoplasma gondii was celebrated. however, toxoplasmosis is still seen as a neglected and underreported disease, despite having a disease burden similar to that of salmonellosis and campylobacteriosis. human vaccines are not available and current antiparasitic treatment is disappointing. this has led to an urge to focus more on prevention. food, soil or water contaminated with oocysts from cat faeces and undercooked meat from infected intermediate host ... | 2009 | 18951847 |
| seroprevalence and epidemiological correlates of toxoplasma gondii infections among patients referred for hospital-based serological testing in doha, qatar. | abstract: | 2008 | 18937857 |
| specific ige to allergens in cord blood is associated with maternal immunity to toxoplasma gondii and rubella virus. | various studies have found reduced prevalences of atopic sensitization and atopic diseases in children previously exposed to infections or living conditions with a high microbial burden, such as the farming environment. | 2008 | 18925886 |
| isolation of toxoplasma gondii from animals in durango, mexico. | little is known concerning the epidemiology of toxoplasma gondii infection in people and animals in rural mexico. serum samples and tissues from 150 dogs (canis familaris), 150 cats (felis catus), 65 opossums (didelphis virginianus), 249 rats (rattus spp.), 127 mice (mus musculus), and 69 squirrels (spermophilus variegatus) from the durango area were evaluated for t. gondii infection. using a modified agglutination test and a serum dilution of 1:25, antibodies to this parasite were found in 68 ( ... | 2009 | 18925790 |
| direct high-resolution genotyping of toxoplasma gondii in arctic foxes (vulpes lagopus) in the remote arctic svalbard archipelago reveals widespread clonal type ii lineage. | characterization of toxoplasma gondii genotypes in hosts living in remote, isolated regions is important for elucidating the population structure and transmission mode of this parasite. herein, we report the results of direct genotyping of t. gondii in brain tissue of arctic foxes (vulpes lagopus) from the remote, virtually cat-free, high arctic islands of svalbard. dna extracts from brains of 167 seropositive arctic foxes (including four cases of fatal toxoplasmosis) and 11 seronegative arctic ... | 2008 | 18922642 |
| isolation of toxoplasma gondii from the keel-billed toucan (ramphastos sulfuratus) from costa rica. | pectoral muscles from a captive keel-billed toucan (ramphastos sulfuratus) from costa rica were fed to a toxoplasma gondii-free cat, and the cat shed oocysts. laboratory mice fed these oocysts developed antibodies to t. gondii in their sera and t. gondii tissue cysts in their brains. the dna extracted from the brains of infected mice was characterized using 10 polymerase chain reaction-restricted fragment length polymorphic markers (sag1, sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and apic ... | 2009 | 18847295 |
| prevalence of toxoplasma gondii in stray cats of gyeonggi-do, korea. | toxoplasma gondii is an obligate intracellular zoonotic protozoan with a worldwide distribution. it infects humans as well as a broad spectrum of vertebrate hosts. cats and wild felidae play crucial roles in the epidemiology of toxoplasmosis. this study was performed to survey the prevalence of t. gondii infection among stray cats in the gyeonggi-do, republic of korea. a total of 174 stray cat blood samples were collected from gwacheon-si (n=20), bucheon-si (82), and yangju-si (72). positive ser ... | 2008 | 18830064 |
| toxoplasma gondii infection in pregnant women in china. | infection with toxoplasma gondii is common and usually asymptomatic, but it can have serious consequences in pregnant women if passed to the developing fetus. the aims of this study were to determine the prevalence of toxoplasmosis in pregnant women and to identify the possible risk factors associated with t. gondii infection in china. of a sample of 235 pregnant women in changchun, china, 25 (10.6%) were found by elisa to be positive for igg and none (0%) for igm. major risk factors were found ... | 2009 | 18822439 |
| fatal toxoplasmosis in sand cats (felis margarita). | the sand cat (felis margarita) is a small-sized felid occurring in the united arab emirates (uae). the sand cat captive-breeding program at the breeding centre for endangered arabian wildlife in sharjah, uae, has until recently been severely compromised by very high newborn mortality rates. two different pairs of sand cats gave birth, respectively, to one and two litters (with a total of eight kittens) between 1999 and 2006. seven out of eight kittens died between the third and 21st wk of life. ... | 2008 | 18816998 |
| prevention of shedding and re-shedding of toxoplasma gondii oocysts in experimentally infected cats treated with oral clindamycin: a preliminary study. | this work aimed to evaluate the effects of preventive oral clindamycin in cats infected with toxoplasma gondii. twelve short hair cats were divided into two groups (group 1 and group 2). no titres of t. gondii antibodies were detected in these cats before the experiment. the animals from group 1 were infected with tissue cysts of t. gondii and group 2 were infected and treated with clindamycin (20 mg/kg/day). the infection was done with almost 40-50 tissue cysts for each cat on day 0. the cats f ... | 2009 | 18811675 |
| frequency and risk factors for toxoplasmosis in ovines of various regions of the state of colima, mexico. | infection of ewes by toxoplasma gondii may induce abortions, thus it has consequences for sheep production. colima, western state of mexico, has favourable climatic conditions for transmission and both domestic and wild cats live there. the aim of this study was to determine the frequency of specific antibodies in sheep from the coast, a mountain, and a hill of colima. serum samples from 351 sheep were tested by a previously standardized indirect elisa. the frequency of infection was estimated a ... | 2008 | 18801208 |
| spinal cryptococcoma in an immunocompetent cat. | this report describes an unusual case of primary cryptococcoma in the proximal thoracic spinal cord of an 11-year-old immunocompetent cat from a farm on which there were large numbers of pigeons. this animal was referred for examination with progressive paralysis and shown to be free from feline immunodeficiency virus, feline leukaemia virus, feline coronavirus and toxoplasma gondii. it died 2 months later. at necropsy, the only lesion detected was a malacic area, 4cm in length, in the spinal co ... | 2008 | 18783789 |
| the immunobiology of the innate response to toxoplasma gondii. | toxoplasma gondii is a unique intracellular parasite. it can infect a variety of cells in virtually all warm-blooded animals. it has a worldwide distribution and, overall, around one-third of people are seropositive for the parasite, with essentially the entire human population being at risk of infection. for most people, t. gondii causes asymptomatic infection but the parasite can cause serious disease in the immunocompromised and, if contracted for the first time during pregnancy, can cause sp ... | 2009 | 18775432 |
| prevalence of antibodies against toxoplasma gondii in domestic cats from mexico city. | in order to determine the frequency of anti-toxoplasma gondii igg antibodies in domestic cats and to identify the possible risk factors, 169 domestic cats from 16 municipalities of mexico city were studied. their serum was tested by indirect elisa and the owners answered a questionnaire about age, gender, litter box hygiene, contact with other cats, housing (indoor, outdoor) and diet. we found 37 (21.8%) seropositive cats to t. gondii with an increase in frequency related to age. main risk facto ... | 2008 | 18774649 |
| toxoplasma gondii transmission from cats to children. | 1981 | 18748842 | |
| prevalence of toxoplasma gondii infection in belgian house cats. | five hundred and sixty seven sera of healthy house cats aged 3 months to 7 years, were examined for the presence of anti-toxoplasma antibodies by indirect immunofluorescence assay and compared to sag1 and tla enzyme linked immunosorbent assays as alternative test. twenty-five percent of cats tested positive for igg and/or igm. seroprevalence increased with age from 2% below 12 months of age up to 44% at age 7. sensitivities of sag1 and tla elisa were 84.1% and 88.6%, respectively. peak levels in ... | 2008 | 18707811 |
| evaluation of immunization with tachyzoite excreted-secreted proteins in a novel susceptible mouse model (a/sn) for toxoplasma gondii. | toxoplasma gondii is an important food-borne parasite transmitted primarily from animals to humans through meat consumption, mainly pork and lamb, as well as through oocysts shed by cats. infection in humans can cause severe neonatal malformations, ocular complications or encephalitis. toxoplasmosis infection during pregnancy, especially in sheep, often results in abortion, representing considerable economic loss. the aim of this study was to investigate whether toxoplasma gondii pooled excreted ... | 2008 | 18706414 |
| control of the risk of human toxoplasmosis transmitted by meat. | one-third of the human world population is infected with the protozoan parasite toxoplasma gondii. recent calculations of the disease burden of toxoplasmosis rank this foodborne disease at the same level as salmonellosis or campylobacteriosis. the high disease burden in combination with disappointing results of the currently available treatment options have led to a plea for more effective prevention. in this review we describe toxoplasma as a hazard associated with the consumption of undercooke ... | 2008 | 18694755 |
| toxoplasmosis in pregnant women in crete. | in greece there are no official recommendations concerning the management of pregnant women for the prevention of congenital toxoplasmosis. a protocol for monitoring pregnant women was designed in order to differentiate between acute and latent toxoplasmosis and was tested successfully for 7 years. the maternofetal transmission rate in crete was assessed and a map showing seroprevalence of pregnant women in all prefectures of greece was prepared. the high seroprevalence of toxoplasma gondii in g ... | 2007 | 18689233 |
| undercooked meat consumption remains the major risk factor for toxoplasma infection in serbia. | toxoplasma gondii infection prevalence and infection risk factors were analyzed in a series of 765 women of generative age from throughout serbia tested at the institute for medical research toxoplasmosis laboratory between 2001 and 2005. the infection risk factors were additionally analyzed in a group of 53 women with acute infection (cases) compared to a group of seronegative women matched for age and education level (controls). the overall prevalence of infection was 33%. infection risk facto ... | 2007 | 18689232 |
| does vertical transmission contribute to the prevalence of toxoplasmosis? | toxoplasma gondii is a ubiquitous parasite with a widespread distribution both in terms of geographical and host range. although the definitive host is the cat, it is also a major health hazard to domestic animals and humans. three routes of transmission are recognised (infection from the cat, carnivory and congenital transmission). we aimed to assess the relative importance of congenital transmission, using sheep as a model system, due to the lack of carnivory. we report, using pcr as a diagnos ... | 2007 | 18689231 |
| ovine toxoplasmosis: transmission, clinical outcome and control. | toxoplasma gondii is a significant cause of abortion in sheep. infection is picked up from the environment and if initiated during pregnancy may cause fetal mortality. infected sheep remain persistently infected with tissue cysts in brain and muscle (meat), and are also immune and would not be expected to abort again. the live tachyzoite vaccine (toxovax) protects against abortion and this allows the suggestion that it may also reduce or prevent tissue cyst development in muscle. if this were so ... | 2007 | 18689230 |
| methods for inactivation of toxoplasma gondii cysts in meat and tissues of experimentally infected sheep. | this study utilized infectivity bioassays in cats and mice to assess the efficacy of inactivation of toxoplasma gondii cysts in experimentally infected sheep meat and tissues subjected to chilling, freezing, heating, microwave cooking, and gamma ray irradiation. heating at 60 degrees c or 100 degrees c for 10 minutes, freezing at either -10 degrees c for 3 days or -20 degrees c for 2 days, or irradiation at doses of 75 or 100 krad was sufficient to kill tissue cysts. meanwhile, neither cooking i ... | 2008 | 18681796 |
| toxoplasma gondii: an improved rat model of congenital infection. | the objective of this study was to refine the rat model of congenital toxoplasmosis. in fischer rats we found that visualization of spermatozoa in vaginal exudates and the detection of at least 6g body weight increase between days 9 and 12 of pregnancy, allowed the diagnosis and timing of pregnancy with 60% specificity and 84% sensitivity. a dose of 10(4) toxoplasma gondii bradyzoites or 10(2) t. gondii oocysts of the prugniaud strain resulted in more than 50% of congenital infection of the rat ... | 2008 | 18634785 |
| specificity of the toxoplasma gondii-altered behaviour to definitive versus non-definitive host predation risk. | the hypothesis that the parasite toxoplasma gondii manipulates the behaviour of its intermediate rat host in order to increase its chance of being predated specifically by its feline definitive host, rather than a non-definitive host predator species, was tested. the impact of a range of therapeutic drugs, previously demonstrated to be effective in preventing the development of t. gondii-associated behavioural and cognitive alterations in rats, on definitive-host predator specificity was also te ... | 2008 | 18620624 |
| seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states. | toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ... | 2008 | 18605803 |
| quantitative toxoplasma gondii oocyst detection by a modified kato katz test using kinyoun staining (kkk) in me49 strain experimentally infected cats. | we detected toxoplasma gondii oocysts in feces of experimentally infected cats, using a kato katz approach with subsequent kinyoun staining. animals serologically negative to t. gondii were infected orally with 5 x 10(2) mice brain cysts of me49 strain. feces were collected daily from the 3rd to the 30th day after challenge. oocysts were detected by qualitative sugar flotation and the quantitative modified kato katz stained by kinyoun (kkk). in the experimentally infected cats, oocysts were dete ... | 2008 | 18604418 |
| prevalence and risk factors for anti-toxoplasma gondii antibodies in goats of the seridó oriental microregion, rio grande do norte state, northeast region of brazil. | the prevalence and risk factors for anti-toxoplasma gondii antibodies were investigated in goats of the seridó oriental microregion, rio grande do norte state, northeast region of brazil. three hundred and sixty-six blood samples from goats collected by jugular venopuncture were used. for the serologic diagnosis of toxoplasma gondii infection, the indirect fluorescent-antibody test (ifat) with cut-off value 1:64 was carried out. the prevalence of anti-t. gondii antibodies was 30.6% [95% ci=25.9- ... | 2008 | 18583058 |
| seroprevalence of antibodies to toxoplasma gondii in gordon's wildcat (felis silvestris gordoni) in the middle east. | most species of felids tested have been found to be the definitive host for toxoplasma gondii. gordon's wildcat (felis silvestris gordoni) is a threatened species found in the united arab emirates (uae) and oman. antibodies to t. gondii were found in all 29 captive and 2 of 7 wild-caught f. s. gordoni in uae examined by the modified agglutination test (mat). titers were 1:100 in 1, 1:200 in 5, 1:400 in 6, 1:800 in 10, 1:1,600 in 5, and 1:3,200 or higher in 4. none of these cats was ill, despite ... | 2008 | 18576866 |
| toxoplasmosis in wallabies (macropus rufogriseus and macropus eugenii): blindness, treatment with atovaquone, and isolation of toxoplasma gondii. | australasian marsupials, especially wallabies, are highly susceptible to clinical toxoplasmosis. this paper describes the use of atovaquone for effective treatment of toxoplasmosis in 4 bennett's wallabies (macropus rufogriseus), along with the serology, isolation of toxoplasma gondii, and genotyping of 3 t. gondii isolates from 5 captive wallabies. this is the first report of success in treating acute toxoplasmosis in wallabies, the first report of serology followed over a significant period of ... | 2008 | 18576797 |
| isolation of toxoplasma gondii from bottlenose dolphins (tursiops truncatus). | toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment and coastal waters with oocysts. in previous serological surveys, >90% of bottlenose dolphins (tursiops truncatus) from the coasts of florida, south carolina, and california had antibodies to t. gondii by the modified agglutination test (mat). in the present study, attempts were made to isolate t. gondii from dead t. truncatus. during 2005, 2006, and 2007, serum or blood clot, and ... | 2008 | 18576793 |
| seroprevalence of toxoplasma gondii infection and risk factors associated with seropositivity of pregnant women in korea. | a total of 351 serum samples was examined for anti-t. gondii antibody titers; the overall seroprevalence was 3.7%. the sabin-feldman dye test (dt), latex agglutination test (lat), and igg enzyme-linked immunosorbent assay (igg elisa) indicated seroprevalences of 3.7%, 3.4%, and 4.0%, respectively. compared with the dt, the sensitivities of the lat and igg elisa were 84.6% and 100.0%, respectively, and the specificities of the lat and igg elisa were both 99.7%. an increase in t. gondii seropreval ... | 2008 | 18576787 |
| molecular characterization of toxoplasma gondii isolates from cats in spain. | cats are important in the epidemiology of toxoplasma gondii because felids are the only definitive hosts that can excrete environmentally resistant oocysts. fresh samples of brain from 103 spanish cats with antibodies to t. gondii were analyzed for t. gondii dna using nested-pcr; 47 (45.5%) were found to be positive. further characterization of dna from 46 cats using rflp-pcr at the 3' and 5' ends of the sag2 locus revealed that 12 (26%) isolates were type i and 34 (74%) were type ii; no type ii ... | 2008 | 18576763 |
| investigation of anti-toxoplasma gondii antibodies in cats of the ankara region of turkey using the sabin-feldman dye test and an indirect fluorescent antibody test. | blood samples from 99 cats from the ankara province of turkey were examined for the presence of anti-toxoplasma gondii antibody with the use of both the sabin-feldman dye test (dt) and an indirect fluorescent antibody test (ifat). forty of the 99 sera (40.3%) were positive for antibodies against t. gondii with the dt, whereas the ifat assay detected antibodies in 34 (34.3%). the study also evaluated 3 factors for their potential association with the presence of t. gondii antibody: age (<1 yr, 1- ... | 2008 | 18576761 |