Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [changes of hypothalamo-pituitary-thyroid function after open heart surgery--especially evaluated by trh test]. | changes of pituitary and thyroid functions after operation were studied in the cases with cardiopulmonary bypass (ecc group) and without bypass (control group). to know mechanisms of these changes, reaction of hypothalamo-pituitary-thyroid axis by using thyrotropin-releasing hormone (trh) test and influence of serum cortisol level were examined. serum concentrations of free t3, free t4 and tsh decreased after open heart surgery. the levels of tsh and free t4 were recovered at the second or third ... | 1991 | 1940515 |
| thyroid hormones in tissues from human embryos and fetuses. | this study was intended to quantify t3 and t4 in various human tissues at different stages of gestation as a contribute in the evaluation of the role of thyroid hormones in fetal development, particularly before the maturation of fetal thyroid function. moreover, for a better comprehension of the influence of thyroid hormone status in tissues, the study was extended to adults. embryonic specimens were obtained from voluntary abortions between 6 and 12 weeks of gestation, fetal and neonatal speci ... | 1991 | 1940061 |
| advantages of a human immunodeficiency virus type 1 (hiv-1) persistently infected hela t4+ cell line for hiv-1 indirect immunofluorescence serology. | a hela t4+ cell line persistently infected with human immunodeficiency virus type 1 (hiv-1) was used in an indirect immunofluorescent antibody assay (ifa) system to explore its potential suitability as an alternative source of viral antigen for confirmatory ifa in hiv serology. in a study of 121 serum samples chosen because they were reactive on repeat examination by enzyme immunoassay but nonspecific by ifa by using hiv-1-infected h9 cells (h9 ifa) or gave discrepant results by enzyme immunoass ... | 1991 | 1939583 |
| interactions between oleic acid and drug competitors influence specific binding of thyroxine in serum. | long chain nonesterified fatty acids and various drugs may share albumin-binding sites in common. we questioned whether serum binding of t4 could be indirectly influenced by displacement of drug competitors from these sites by nonesterified fatty acids. the influence of oleic acid on drug-induced inhibition of [125i]t4 binding was measured by equilibrium dialysis, using undiluted serum in order to avoid dilution-related artefacts. oleic acid (1 mmol/l) alone did not inhibit serum protein binding ... | 1991 | 1939526 |
| dna footprinting studies of the complex formed by the t4 dna polymerase holoenzyme at a primer-template junction. | we have used dna footprinting techniques to analyze the interactions of five dna replication proteins at a primer-template junction: the bacteriophage t4 dna polymerase (the gene 43 protein), its three accessory proteins (the gene 44/62 and 45 proteins), and the gene 32 protein, which is the t4 helix-destabilizing (or single-stranded dna-binding) protein. the 177-nucleotide-long dna substrate consisted of a perfect 52-base pair hairpin helix with a protruding single-stranded 5' tail. as expected ... | 1991 | 1939067 |
| the t4 dna polymerase accessory proteins form an atp-dependent complex on a primer-template junction. | the dna polymerase holoenzyme of bacteriophage t4 contains, besides the dna polymerase itself (the gene 43 protein), a complex of the protein products of t4 genes 44 and 62 (a dna-dependent atpase) and of gene 45. together, the 44/62 and 45 proteins form an atp-dependent "sliding clamp" that holds a moving dna polymerase molecule at the 3' terminus of a growing dna chain. we have used a unique dna fragment that forms a short hairpin helix with a single-stranded 5' tail (a "primer-template juncti ... | 1991 | 1939066 |
| the product of the split suny gene of bacteriophage t4 is a processed protein. | the suny gene of bacteriophage t4 contains a self-splicing group i intron. the ligated exons encode an open reading frame of 605 amino acids, whose inferred molecular mass is 68 kda. however, none of the proteins made following t4 infection have been assigned to the suny gene, and no mutations have been mapped to this locus. we show here that the primary product of the suny gene is a protein with an apparent molecular mass of 64 kda, which is processed to a protein approximately 4 kda smaller. u ... | 1991 | 1938898 |
| eeg and skeletal development in children with different psychosocial characteristics. | two groups of children with different socioeconomic level were studied. one minute eeg at rest was recorded in monopolar leads f3, f4, c3, c4, p3, p4, o1, o2, f7, f8, t3, t4, t5 and t6. absolute and relative power in four eeg bands (delta, theta, alpha and beta) were computed. radiographies of the left hand and the wrist were also obtained in all children. age regression equations of the variables derived from eeg spectra were calculated in each group. in the group with low socioeconomic level m ... | 1991 | 1938172 |
| phage t4 expression vector: protection from proteolysis. | we have developed an efficient method for the expression of heterologous genes during infection by t4, a bacteriophage known to inhibit the proteolytic systems of escherichia coli. this system enables us to clone genes in a plasmid expression vector and move them readily into t4. we have used lacz as a reporter gene to show that both plasmid and phage exhibit low basal expression or high-level expression under the control of a t7 promoter. this system promises a possible solution to the problem ... | 1991 | 1937029 |
| promoter recognition by the rna polymerase from vegetative cells of the cyanobacterium anabaena 7120. | the transcription start points (tsp) of seven genes of anabaena 7120 were previously identified by s1 nuclease protection and primer extension experiments using rna extracted from cells. in the present work, these tsp were confirmed, with one exception, by in vitro transcription using purified rna polymerases of anabaena 7120 and escherichia coli, and crude extracts of anabaena 7120 active in transcription. in all cases, the template for transcription consisted of closed circular plasmid dna in ... | 1991 | 1937007 |
| the effects of thyroid hormones and starvation on hepatic mitochondrial nucleic acids of rainbow trout (oncorhynchus mykiss). | twenty four hours after an intraperitoneal injection of thyroxine (t4; 4.4, 44 ng/g body wt) or triiodothyronine (t3; 3.3, 33 ng/g body wt), dna and rna were significantly reduced in isolated liver mitochondria of rainbow trout. total liver dna was increased with the higher doses in both t4- and t3-injected specimens while total rna was significantly reduced with the same doses. total circulating plasma t4 was reduced with the injections of t3 or starvation, and plasma t3 was increased severalfo ... | 1991 | 1936929 |
| synthesis and examination of antigenicity of four hormonogenic sites and two non-hormonogenic sites of human thyroglobulin. | four peptides (htg-1, 1-10; htg-2, 2547-2558; htg-4, 2592-2603; htg-6, 2737-2748) which contain hormonogenic acceptor tyrosine (tyr) residues and two control peptides (htg-3, 2582-2591; htg-5, 2687-2694) of human thyroglobulin (tg) were synthesized, radioiodinated and their binding with serial anti-human tg antisera which had been raised in two rabbits (tg-1, tg-2) tested. although increased binding of each of the six peptides was observed, htg-4 and htg-2 had higher binding whereas htg-1 and ht ... | 1991 | 1936549 |
| functional properties of human thyroid hormone receptor beta 1 overexpressed using baculovirus. | we have overexpressed the human beta 1 thyroid hormone receptor in insect cells using a recombinant baculovirus to a level of 5-10% of total cellular protein. the recombinant protein migrates as a 50 kda band by sds-page and western blot analysis. the expressed receptor binds to l-t3 with a kd of 1.3 +/- 0.4 x 10(-10) m and to thyroid hormone analogues with an affinity hierarchy of triac greater than l-t3 greater than l-t4 greater than rt3. gel retardation assays show highly specific receptor bi ... | 1991 | 1936281 |
| outer ring iodothyronine deiodinases and thyroid hormone economy: responses to iodine deficiency in the rat fetus and neonate. | female rats were fed a low iodine diet (lid) or the same diet supplemented with ki (iod) and mated. plasma tsh, t4 and t3 in thyroid, plasma, and tissues, and 5'-deiodinase activities (5'd) were measured in maternal, fetal, and neonatal samples. plasma t4 was markedly reduced in lid dams, tsh was increased, and t3 was normal. placental t4 was decreased to 10%, and placental t3 to 50%. in lid fetuses there was a complete depletion of both extrathyroidal and intrathyroidal stores of t4 and t3. the ... | 1991 | 1935795 |
| effect of the exposure to chronic-intermittent cold on the thyrotropin and thyroid hormones in the rat. | rats exposed to acute cold (4 degrees c for 2 h), chronic cold (4 degrees c), and chronic-intermittent cold (4 degrees c for 2 h daily) were killed after 1, 2, 3, 4, and 10 days of cold exposure. the control group was maintained at 25 degrees c. in each animal, the plasma concentration of thyrotropine (ths), triiodothyronine (t3), and thyroxine (t4) was determined by radioimmunoassay. at the initial time of exposure, elevations in tsh, t3, and t4 were observed in the rats in each experimental gr ... | 1991 | 1935178 |
| [htlv-i associated myelopathy with hashimoto's thyroiditis--a report of two cases]. | we reported two cases of htlv-i associated myelopathy with hashimoto's thyroiditis. case 1 is a 47-year-old female, who has had hashimoto's thyroiditis since the age of 29. at the age of 38, she first noticed difficulty in walking which progressed gradually. at the time of examination, she showed a spastic gait, increased deep tendon reflexes in four extremities, hypesthesia of the feet, mild bowel and bladder disturbances. anti-htlv-i antibody was increased to the titer of 16,834 in the serum a ... | 1991 | 1934781 |
| [a case of phenytoin intoxication induced by hypothyroidism]. | a 42-year-old woman who presented phenytoin intoxication induced by acute hypothyroidism was reported. she had a 29-year history of hypothyroidism and a 18-year history of epilepsy. she was treated with phenytoin (pht) 100 mg, mephobarbital (mpb) 200 mg, valproic acid (vpa) 400 mg and thyroid powder 100 mg daily for 2 years. she had no medical problem until she noticed gait disturbance and diplopia which appeared 1 month after sudden withdrawal of thyroid powder. on admission, she was somnolent ... | 1991 | 1934766 |
| triac (3,5,3'-triiodothyroacetic acid) has parallel effects at the pituitary and peripheral tissue levels in thyroid cancer patients treated with l-thyroxine. | to investigate whether the addition of 3,5,3'-triiodothyroacetic acid (triac) to thyroxine (t4) treatment can suppress tsh secretion without inducing thyrotoxicosis at the periphery. | 1991 | 1934526 |
| tumor-infiltrating lymphoreticular cells. histologic and immunohistologic investigations performed on metastasizing squamous cell carcinomas of the head and neck. | ten squamous cell carcinomas of the head and neck region and metastatic deposits in 41 cervical lymph nodes were investigated histologically and immunohistologically to determine the cellular immune response to the tumors, particularly the localization and phenotype of the tumor-infiltrating lymphoreticular cells. the lymphoreticular cells were accumulated preferentially in the stroma in and around the tumor. the foci of neoplastic cells usually contained smaller numbers of lymphoreticular cells ... | 1991 | 1933776 |
| the trh stimulation test in alzheimer's disease and major depression: relationship to clinical and csf measures. | a blunted thyroid-stimulating hormone (tsh) response to exogenous thyrotropin-releasing hormone (trh) has been reported to occur consistently in patients with major depression and less consistently in patients with alzheimer's disease (ad). in this study we compared the tsh response to trh in a large group (n = 40) of ad patients, elderly patients with major depression (n = 17), and age-matched controls (n = 14) to further characterize how it may relate to clinical variables, baseline thyroid fu ... | 1991 | 1932406 |
| metabolic response to colonic surgery: extradural vs continuous spinal. | we have examined the effect of intraoperative and postoperative (4 h) continuous spinal anaesthesia for colonic surgery on the postoperative glucose, lactate and cortisol responses. twenty-one patients were studied; the first group (control) received general anaesthesia, the second group (extradural) an extradural block (t4-s5) and the third group (spinal) a continuous spinal block (t4-s5). plasma concentration of glucose increased significantly in the control and extradural groups (p less than ... | 1991 | 1931405 |
| the effects of thyroid hormones on potassium-stimulated release of 3h-gaba by synaptosomes of rat cerebral cortex. | depolarization-induced release of neurotransmitters from nerve endings is triggered by the rapid entry of ca through voltage-sensitive channels. low (1 to 10 nmol/l) concentrations of l-triiodothyronine (t3), but not d-t3, reverse t3 (rt3) or l-thyroxine (t4), enhance fast-phase depolarization-induced 45ca uptake by rat brain synaptosomes. in the present study the effects of thyroid hormones on the release of 3h-gamma-aminobutyric acid (3h-gaba) by cerebral cortical synaptosomes from adult male ... | 1991 | 1930611 |
| microfluorometric size measurements of human and phage dna correlate with the degree of in vitro cisplatin treatment. | the anticancer drug cisplatin is shown to inhibit the proofreading activity of the enzyme t4 polymerase. a microfluorometric assay that can measure the relative sizes of cisplatin treated human and phage dna, after exposure to t4 polymerase, is described. using phage dna size standards, it is shown that the mean integrated fluorescent density (ifd) of fluorophore labeled dna is linearly correlated with molecular size. exonuclease digestion by t4 polymerase of cisplatin treated lambda dna gave fr ... | 1991 | 1930179 |
| [the virulence of l. leucocephala for goats in northeastern mexico]. | when fed 100% leucaena during a period of 45 days goats with experience in l.-fodder took significantly (p less than 0.05) more dry matter (50.01 g/kg0.75) than those without or only possible experience (44.40 g/kg0.75 and 45.63 g/kg0.75). the uptake of mimosine from animals with mimosine experience (1.14 g/kg0.75) has also been significantly higher (p less than 0.05) than from two other groups with non or only possible experience (0.93 g/kg0.75 and 0.92 g/kg0.75). the serum-thyroxin level (t4) ... | 1991 | 1930109 |
| nodal recurrence in primary malignant epithelial tumours of the parotid gland. | an analysis has been made of the incidence of nodal recurrence in 237 patients with primary carcinoma of the parotid gland. fifty eight patients (24.4%) developed nodal metastases; mostly in the cervical region. eighteen of these metastases were within the radiation field and 13 were outside the radiation field. factors which were predictive for nodal relapse were: age greater than 60 years, t3 and t4 tumours, node metastases at initial presentation, and macroscopic disease at either the primary ... | 1991 | 1930017 |
| [congenital hypothyroidism. therapeutic strategy at the early stage of treatment]. | the aim of this work was to determine the optimum dosage of l-thyroxine (l-t4) given to infants with congenital hypothyroidism (ch). thirty seven hypothyroid infants diagnosed through the french screening programme for ch have been treated in our clinic. the study analysed the biochemical parameters (tsh, ft4, ft3) and the l-t4 doses during the first year of life. treatment was started at 23 days of age (range: 13 to 37). a dose of 7.5 micrograms/kg/d of l-t4 was given at diagnosis. after 2 week ... | 1991 | 1929745 |
| thyroid hormone changes after cardiovascular surgery and clinical implications. | alterations in serum concentrations of total triiodothyronine (tt3), total thyroxine (tt4), and thyroid-stimulating hormone (tsh) frequently occur in patients with nonthyroidal illnesses. these changes correlate with the severity of the illness and the prognosis. in this study, 44 patients undergoing a cardiovascular operation had significant declines in serum tt3 and tt4 levels during cardiopulmonary bypass and thereafter. serum tt3 and tt4 concentrations reached their nadir at 30 minutes after ... | 1991 | 1929631 |
| prognostic clinicopathologic factors in cutaneous t-cell lymphoma. | influence of clinicopathologic data on survival was analyzed in 43 patients with cutaneous t-cell lymphoma. the median age was 66 years; 35 were male and eight female. the extent of the disease, established according to a modification of the tnm system, was as follows: t1, three patients; t2, 15; t3, 14; t4, 11; n0, 15; n1, 28; m0, 38; m1, 5; b0, 37; and b1, six. the first treatment applied after staging was skin-limited therapy in seven patients and different regimens of systemic chemotherapy i ... | 1991 | 1929458 |
| low serum t3 and raised reverse t3 levels in hepatic cirrhosis: role of glucagon. | hepatic parenchymal tissue is known to be one of the major sites of thyroid hormone metabolism as well as glucagon action. alterations in circulating thyroid hormone concentrations, as well as hyperglucagonemia, are well documented in subjects with hepatic cirrhosis and advanced liver dysfunction. also, we have documented recently that hyperglucagonemia induced in normal subjects alters thyroid hormone metabolism, with lowering of serum t3 and a rise in serum reverse t3 (rt3) levels. thus, it is ... | 1991 | 1928046 |
| the effect of propranolol on thyroid function in patients with liver cirrhosis. | the effect of propranolol on thyroid hormones of 7 healthy subjects and 10 patients with histologically proven alcoholic liver cirrhosis was investigated. the fractions of plasma t3 and free t4 were determined by specific radioimmunoassay before and after two weeks of propranolol administration. under basal conditions, both t3 and ft4 levels were found significantly lower in patients with cirrhosis than in healthy subjects (1.86 +/- 0.10 vs. 1.18 +/- 0.41 nmol/l, p less than 0.001; 9.31 +/- 0.41 ... | 1991 | 1926961 |
| characterization of peripheral blood t-cell subpopulation of bladder cancer patients. | the levels of immune reactivity of peripheral and blood t-lymphocytes were evaluated in 37 bladder cancer patients and 31 age-matched controls. t-lymphocyte subsets were quantified by monoclonal antibodies, and the immune reactivity was measured using stimulation with phytohemagglutinin (pha), concanavalin a (cona), and pokeweed mitogen (pwm). comparing the patients before and after treatment revealed significant changes in the stimulation index of proliferative response to pha, pwm, in the pwm% ... | 1991 | 1926656 |
| biological structures imaged in a hybrid scanning transmission electron microscope and scanning tunneling microscope. | a hybrid scanning transmission electron microscope (stem) and scanning tunneling microscope (stm) is described which allows simultaneous imaging of biological structures adsorbed to electron-transparent specimen supports in both modes of scanning microscopy, as demonstrated on uncoated phage t4 polyheads. we further discuss the reproducibility and validity of height data obtained from stm topographs of biomacromolecules and present raw data from topographs of freeze-dried, metal-coated nuclear e ... | 1991 | 1926633 |
| [low t3 and low t3-t4 syndrome]. | 1991 | 1925242 | |
| [peridural anesthesia using high volume prilocaine--success rate and patient acceptance. a prospective study of 630 anesthetized patients]. | in a prospective clinical study the follow-up course of 630 lumbar single-shot and continuous epidural anesthetics in young patients (average age 24.5 years) with 20-25 ml prilocaine 2% (epinephrine concentration 1:200,000) and 0.1 mg fentanyl was examined critically. the patients were interviewed postoperatively about this method of anesthesia. in 89% of cases the patients had been found suitable for surgery without any additional medication; 9% of the patients needed 0.1-0.15 mg fentanyl and 2 ... | 1991 | 1924909 |
| the human homologous pairing protein hpp-1 is specifically stimulated by the cognate single-stranded binding protein hrp-a. | homologous pairing and strand exchange of dna are catalyzed by the human homologous pairing protein hpp-1 in a magnesium-dependent, atp-independent reaction that requires homologous dna substrates and stoichiometric quantities of hpp-1. here we show that the addition of the purified human single-strand binding (ssb) protein hrp-a to the reaction mixture stimulates the rate of homologous pairing 70-fold and reduces the amount of hpp-1 required for the reaction at least 10-fold. the identification ... | 1991 | 1924369 |
| antibiotic inhibition of group i ribozyme function. | the discovery of catalytically active rna has provided the basis for the evolutionary concept of an rna world. it has been proposed that during evolution the functions of ancient catalytic rna were modulated by low molecular weight effectors, related to antibiotics, present in the primordial soup. antibiotics and rna may have coevolved in the formation of the modern ribosome. here we report that a set of aminoglycoside antibiotics, which are known to interact with the decoding region of the 16s ... | 1991 | 1922343 |
| an essential role of domain d in the hormone-binding activity of human beta 1 thyroid hormone nuclear receptor. | by analogy with steroid receptors, human placental thyroid hormone nuclear receptor (htr beta 1) could be divided into four functional domains: a/b (met1-leu101), c (cys102-ala170), d (thr171-lys237), and e (arg238-asp456). the e domain was thought to bind thyroid hormone. to evaluate whether domain e alone is sufficient to bind t3 or requires the presence of other domains for functional t3-binding activity, a series of deletion mutants was constructed. the mutants were expressed in escherichia ... | 1991 | 1922081 |
| thyroid hormone decreases the stability and the poly(a) tract length of rat thyrotropin beta-subunit messenger rna. | thyroid hormone (t3 and t4) down-regulation of tsh subunit steady state mrna levels and subunit gene transcription in vitro and in vivo has been well studied. we present evidence here that t3 can also regulate the turnover of tsh subunit mrna. the apparent half-life of the tsh beta-subunit mrna was determined by adding actinomycin-d (2 microm) to dispersed rat pituitary cultures in hypothyroid medium or medium containing 10(-7) m t3 and analyzing the decline in subunit mrna levels with time. the ... | 1991 | 1922079 |
| immunoelectron microscopy of cell populations in regional and central lymph of sheep. | the immunoreactivity and the ultrastructural localization of monoclonal anti-sheep lymphocyte antibodies conjugated with colloidal gold particles were examined in free-floating cells of sheep central lymph from the thoracic duct, postnodal lymph draining either the popliteal nodes or the mesenteric nodes, and prenodal lymph draining the pregnant uterus. the monoclonal antibodies used in this study were sbu-t1 (cd5), sbu-t4 (cd4), sbu-t8 (cd8), sbu-ii (anti dr antibody), and e53 which are reporte ... | 1991 | 1921477 |
| prognostic factors in laryngeal cancer patients submitted to surgical treatment. | the authors studied some demographic, clinical, pathologic, and therapeutic variables in relation to the disease-free interval (dfi) and to the overall survival of laryngeal cancer patients. the patient population consisted of 278 consecutive cases of primary laryngeal cancer who had been admitted to four head and neck surgery services in sao paulo and curitiba (brazil) between 1973 and 1986. all patients had surgical treatment. although several variables exhibited individual associations with s ... | 1991 | 1921404 |
| [a comparison of measurements of serum free thyroxine concentration by equilibrium dialysis and ultrafiltration using undiluted serum]. | we have examined the clinical usefulness for free thyroxine (ft4) measurement by equilibrium dialysis of undiluted serum and by radio-immunoassay of t4 concentration in the dialysate (ed/ria). the results were compared with those by ultrafiltration of undiluted serum (uf/ria), which is believed to offer the best possible approach towards estimation of in vitro serum level of ft4. the precision with both methods was excellent. there was a high degree of correlation of ft4 concentration by both me ... | 1991 | 1920948 |
| inhibition of human immunodeficiency virus type 1-induced cell fusion by recombinant human interferons. | pretreatment of hela t4 cells with recombinant alpha, beta, or gamma interferon (ifn) was found to significantly inhibit syncytium formation induced by the human immunodeficiency virus type 1 (hiv-1) envelope glycoprotein. all three ifns were found to be potent inhibitors of fusion in a system in which spodoptera frugiperda cells, infected with a baculovirus recombinant expressing the hiv-1 envelope protein, were cocultivated with hela t4 cells. in addition, these ifns were also found to block h ... | 1991 | 1920634 |
| structural and thermodynamic analysis of the packing of two alpha-helices in bacteriophage t4 lysozyme. | packing interactions in bacteriophage t4 lysozyme were explored by determining the structural and thermodynamic effects of substitutions for ala98 and neighboring residues. ala98 is buried in the core of t4 lysozyme in the interface between two alpha-helices. the ala98 to val (a98v) replacement is a temperature-sensitive lesion that lowers the denaturation temperature of the protein by 15 degrees c (ph 3.0, delta delta g = -4.9 kcal/mol) and causes atoms within the two helices to move apart by u ... | 1991 | 1920439 |
| platinum/iridium/carbon: a high-resolution shadowing material for tem, stm and sem of biological macromolecular structures. | thin pt/ir/c coating films (1.5 nm) show a fine granularity and provide a high structural resolution in the transmission electron microscope (tem) when applied to freeze-dried biological macromolecules. they keep their structure when exposed to atmospheric conditions, without the need of an additional stabilizing carbon layer, in contrast to conventional high-resolution shadowing materials such as ta/w and pt/c. however, the correct ratio of the components has turned out to be crucial. when evap ... | 1991 | 1920395 |
| high resolution biological scanning electron microscopy: a comparative study of low temperature metal coating techniques. | structural information on the surface of biological specimens can be resolved within molecular dimensions by "in-lens" field emission scanning electron microscopes when cryo-methods are used to adequately preserve the native state of the specimen. the visual definition of molecular surface structures depends largely on the metal coating. the thickness of the coating, as well as the temperature at which it is deposited, are among the most important parameters affecting visual definition. these we ... | 1991 | 1919798 |
| the inter- and intraexaminer reliability of a paraspinal skin temperature differential instrument. | an experiment was undertaken to determine the intra- and interexaminer reliability of a paraspinal skin temperature differential instrument. nineteen pain-free female chiropractic college students participated as subjects for the investigation. three separate areas of the spine (c4-t2, t4-t8 and l2-l5) were examined for concordance between two examiners. additionally, intraexaminer reliability was tested by having each examiner repeat the scanning procedure. concordance for whether a temperature ... | 1991 | 1919373 |
| [serotypes and antibiotic susceptibility of group a and b streptococci clinically isolated in sapporo in the last five years]. | during the last five-year period between 1985 and 1989, serotypes of 671 strains of group a and 238 strains of group b streptococci isolated from clinical specimens in sapporo city general hospital were studied. results of serotyping were given as under; type t4 (25.8%), t12 (25.6%), t1 (11.5%), t3 (9.2%), t6 and t28 (6.3% each), t8 and t22 (0.6% each), t13 (0.4%), t9 and b3264 were found in group a. type t4 and t12 formed main types up to this time and t1, t3, t6 and t28 were other predominant ... | 1991 | 1919128 |
| [isolation trends on group a streptococci isolated from clinical specimens in hospitals in saitama, 1979-1989]. | on 4,864 group a streptococci isolated from throat culture of patients in hospitals in saitama from 1979 to 1989, we have done an epidemiological analysis of the t-type detection rates and the t-type epidemic, the monthly isolation rates, and the isolation rates by sex and age of patients. 1. the t-type detection rates of 4,864 isolates of group a streptococci showed type t12 in 22.6%, type t4 in 16.4%, type t1 in 10.8%, type t13 in 8.5%, type t6 in 7.6%, type t28 in 6.9%, type t3 in 6.1% and ty ... | 1991 | 1919127 |
| the effects of lithium discontinuation and the non-effect of oral inositol upon thyroid hormones and cortisol in patients with bipolar affective disorder. | thyroid and adrenal function was assessed in euthymic bipolar patients, stable on prophylactic lithium for at least 1 year, before and after lithium discontinuation in a randomised double-blind placebo-controlled trial. all hormonal measurements were within the reference range, but a significant increase (p less than 0.001) in plasma thyroxine (t4) levels and a decrease (p less than 0.01) in tsh levels were observed 1 month after lithium withdrawal; cortisol concentrations showed a non-significa ... | 1991 | 1918659 |
| trachyonychia associated with alopecia areata: a clinical and pathologic study. | forty of 1095 patients (3.65%) with alopecia areata had severe nail changes that fulfilled the clinical criteria for the diagnosis of trachyonychia. twelve of these patients had a nail biopsy. a mild to moderately dense lymphocytic infiltrate associated with exocytosis and spongiosis was detected in the proximal nailfold, nail matrix, nail bed, and hyponychium of 11 patients. one patient showed the pathologic changes of lichen planus; lichen planus of the skin developed 6 months after the nail b ... | 1991 | 1918465 |
| role of l-thyroxine in nuclear thyroid hormone receptor occupancy and growth hormone production in cultured gc cells. | the contribution of l-thyroxine (t4) to nuclear thyroid receptor occupancy was studied in gc cells incubated with concentrations of 3,5,3'-triiodo-l-thyronine (t3) and t4 that resulted in free iodothyronine levels similar to those in serum of euthyroid rats. t4 accounted for 5.4-10% of the occupied receptors: t3 derived from t4 [t3(t4)] and t3 added to medium accounted for the remainder of receptor occupancy. incubation with increasing medium free t4 resulted in a progressive increase in the con ... | 1991 | 1918379 |
| transcription from a bacteriophage t4 middle promoter using t4 mota protein and phage-modified rna polymerase. | the bacteriophage t4 mota protein is required for transcription from t4 middle promoters. these promoters, which contain the escherichia coli promoter consensus sequence at the -10 region (tataat) but a unique sequence centered at -30 ((a/t)(a/t)tgctt(t/c)a) (guild, n., gayle, m., sweeney, r., hollingsworth, t., modeer, t., and gold, l. (1988) j. mol. biol. 199, 241-258), become active about 2 min after infection, a time when the host rna polymerase has been modified by phage proteins. this pape ... | 1991 | 1917941 |
| an nmr characterization of the rega protein-binding site of bacteriophage t4 gene 44 mrna. | the conformations of two rna dodecamers that differ markedly in affinity for the rega protein from bacteriophage t4 have been examined by nmr to see if the ability of that protein to discriminate between mrnas is based on pre-existing differences in their three-dimensional structures. one of the rnas examined has the same sequence as the site where rega protein binds when it inhibits the expression of gene 44's mrna. the second rna differs from the first in having a u instead of a g at position ... | 1991 | 1917925 |
| thyroid morphology and function and its role in thermoregulation in the newborn southern elephant seal (mirounga leonina) at macquarie island. | the thyroid gland of the newborn southern elephant seal pup exhibits markedly increased secretory activity during the first 24 hours after birth. thyroid epithelial cell height is cuboidal to columnar for pups from birth to 48 hours postpartum after which it decreases by five days of age. ultrastructurally the thyroid epithelial cells show pseudopodia protruding into the lumen at zero, two and six hours after birth. after 24 hours postpartum pseudopodia are rarely observed in thyroid follicles f ... | 1991 | 1917675 |
| effects of excess iodine in chick embryo thyroid follicles: initial inhibition and subsequent hypertrophy. | the effects of excess iodine on the development of the thyroid gland of chick embryos was assessed following injections of potassium iodide prior to incubation. iodide injection resulted in a significantly greater thyroid gland weight (goitre) on day 18 of incubation and a delay in hatching. histological studies of the thyroid gland on day 12 of incubation revealed that iodide injection had inhibited thyroid follicle development. on day 14, however, the thyroid glands of the iodide-treated embry ... | 1991 | 1917670 |
| an enzymeimmunoassay method for thyroxine determination in serum samples. | here we describe an enzymeimmunoassay (eia) for thyroxine (t4) in serum, whose performance is comparable to that of a sensitive t4 radioimmunoassay (ria). in this assay, specific t4 antibody adsorbed on polystyrene beads is used along with t4-horseradishperoxidase as the tracer and omicron-phenylenediamine as chromogen. several samples were analysed both by this t4 eia and by using a commercial ria kit for t4. the results correlate well with a correlation coefficient r = 0.9 and slope = 0.93 (n ... | 1991 | 1916941 |
| congenital hypothyroidism and pericardial effusion. | a group of infants, affected by congenital hypothyroidism diagnosed through the neonatal screening program, was investigated with echocardiography to detect the presence of pericardial effusion. we studied the relationship between the effusion and the etiology of hypothyroidism, established through thyroid scintiscanning. our data show a high prevalence of effusion in hypothyroid patients, without other clinical signs of cardiac involvement as well as a relationship between the etiology of hypot ... | 1991 | 1916653 |
| marker-dependent recombination in t4 bacteriophage. iii. structural prerequisites for marker discrimination. | distance- as well as marker-dependence of genetic recombination of bacteriophage t4 was studied in crosses between riib mutants with known base sequences. the notion of a "basic recombination," which is the recombination within distances shorter than hybrid dna length in the absence of mismatch repair and any marker effects, was substantiated. the basic recombination frequency per base pair can serve as an objective parameter (natural constant) of general recombination reflecting its intensity. ... | 1991 | 1916240 |
| treatment of hyperthyroidism with potassium iodide. | potassium iodide was given to 17 cases of basedow's disease in comparison to 12 normal controls for 5 weeks. in the thyrotoxic patients, the maximum decrease in serum t3 and t4 levels was observed 2 and 3 weeks, respectively, but in some cases remained normal even at 5 weeks. the euthyroid persons showed a small decrease in t4 and t3 and an increase in serum tsh. | 1991 | 1915648 |
| theoretical and functional analysis of the siv fusion peptide. | the fusion domain of simian immunodeficiency virus (siv) envelope glycoproteins is a hydrophobic region located at the amino-terminal extremity of the transmembrane protein (gp32). assuming an alpha helical structure for the siv fusogenic domain of gp32 in a lipid environment, theoretical studies have predicted that the fusion peptide would insert obliquely in the lipid bilayer. this oblique insertion could be an initial step of the fusion process by disorganizing locally the structure of the li ... | 1991 | 1915259 |
| effect of extracellular sodium on thyroid hormone uptake by mouse thymocytes. | in mouse thymocytes, a stereospecific saturable energy-dependent and ouabain-inhibitable system facilitates t3, but not t4, entry. we studied here the effect of sodium depletion on cellular uptake of thyroid hormones by mouse thymocytes. time-course experiments indicated that extracellular sodium depletion reduced [125i]t3 uptake at each time studied. at equilibrium, the removal of extracellular sodium and its substitution with isoosmotic choline decreased saturable [125i]t3 uptake by 60 +/- 10% ... | 1991 | 1915098 |
| characterization of a n-bromoacetyl-l-thyroxine affinity-labeled 55-kilodalton protein as protein disulfide isomerase in cultured glial cells. | in glial cells, thyroid hormone regulates the polymerization state of the actin cytoskeleton by a mechanism that does not require protein synthesis or the nuclear t3 receptor. using the affinity label n-bromoacetyl-l-t4, we identified a thyroid hormone-binding protein of 55 kda (glial-p55) in cultured glial cells that is unique from the type ii iodothyronine 5'-deiodinase and which exhibits a t4-dependent shift from a membrane-associated pool to the f-actin cytoskeleton. in a number of other cel ... | 1991 | 1915081 |
| a novel nadph-dependent cytosolic 3,5,3'-triiodo-l-thyronine-binding protein (ctbp; 5.1s) in rat liver: a comparison with 4.7s nadph-dependent ctbp. | a novel nadph-dependent cytosolic 3,5,3'-triiodo-l-thyronine (t3)-binding protein (ctbp) was purified by sequential fractionation of rat liver cytosol on q-sepharose, phenyl-sepharose, red-sepharose, and polyacrylamide gel electrophoresis under nondenaturing conditions. the ctbp had a sedimentation coefficient of 5.1s, a stokes' radius of 35 a, and a calculated mol wt of 76,000. the apparently homogenous protein consisted of a dimer of a polypeptide chain with a mol wt of 38,000 as estimated by ... | 1991 | 1915060 |
| conduction velocities and nerve fibre diameters of touch, pain, urinary bladder and anal canal afferents and alpha and gamma-motoneurons in human dorsal sacral roots. | 1. single action potentials were recorded extracellularly at 2 sites from human sacral dorsal nerve roots, and their conduction time measured. conduction velocity frequency distribution histograms were constructed from afferent and efferent nerve fibres. conduction velocity distribution peaks could be identified from afferents of mechanoreceptors of the skin, the bladder, the anal canal, of stretch and flow receptors of the urinary bladder, from spindle afferents and from the 3 alpha (extrafusal ... | 1991 | 1915037 |
| predictive value of a negative computed tomographic scan in 100 patients with rectal carcinoma. | the predictive value of a negative computed tomographic (ct) scan was assessed in a group of 100 patients with rectal carcinoma by correlating operative findings and pathologic stages in the patients who had no evidence of extrapelvic metastases on a preoperative ct scan. sixty-four patients (64 percent) had stage t3 or t4 tumors. ten patients had unsuspected distant metastases for an overall negative predictive value of 90 percent. seven patients had small liver metastases, and three had periao ... | 1991 | 1914737 |
| quantitative and kinetic examination of 32p-postlabeling of etheno-substituted nucleotides. | 1,n6-ethenodeoxyadenosine-, 1,n2-ethenodeoxyguanosine- and 3,n4-ethenodeoxycytidine-3'-monophosphates were labeled by [gamma-32p] atp using t4 polynucleotide kinase in conditions commonly used for the 32p-postlabeling assay. kinetic studies showed that the reaction is fast reaching a plateau after 15-30 min. the efficiency of phosphorylation, as studied by substrate-product concentration dependency, was between 50-100% at the lower substrate concentrations. the adducts are labeled efficiently at ... | 1991 | 1913981 |
| [pregnancy and obesity. ii. hormonal and metabolic changes]. | 28 obese women were investigated in the course of the 21st (a) and 30th (b) week of pregnancy. increased serum levels of gh, t4 and t3 were found in both samples of blood (a and b), while serum insulin, cholesterol and triacylglycerols were increased only at time b. it was suggested that the similar increase of gh, t4 and t3 at time a and b was due to pregnancy because the level of these hormones is usually not increased in non-pregnant obese women, while hyperinsulinemia and often increased val ... | 1991 | 1913857 |
| amiodarone antagonizes the effects of t3 at the receptor level: an additional mechanism for its in vivo hypothyroid-like effects. | amiodarone is a diiodinated benzofuran derivative that has some structural similarities to the thyroid hormones and contains two iodine atoms per molecule. it has exhibited hypothyroid-like effects that are thought to be the result of an inhibition of thyroid hormone synthesis due to iodine load, a decrease in the t4 to t3 conversion, and (or) a competitive binding for t3 receptors. the aim of this study was to determine if this third mechanism contributes to the hypothyroid-like effects of amio ... | 1991 | 1913332 |
| tests of homogeneity for the relative risk in multiply-matched case-control studies. | we examine several tests of homogeneity of the odds ratio in the analysis of 2 x 2 tables arising from epidemiologic 1:r matched case-control studies. the t4 and t5 statistics proposed by liang and self (1985, biometrika 72, 353-358) are unable to detect obvious inhomogeneity in two numerical examples and in simulation studies. the null hypothesis is rejected by the chi-square statistic of ejigou and mchugh (1984, biometrika 71, 408-411) and by a new proposed method whose significance level must ... | 1991 | 1912269 |
| eeg power variation in schizophrenic subgroups: effects of emotionally salient stimuli. | eighteen unmedicated chronic schizophrenic patients and 13 normal controls were tested in a paradigm designed to examine functional changes in electroencephalographic (eeg) activity following presentation of emotionally salient auditory stimuli and control tones. five standard bands of eeg spectral power were examined at bilateral frontal and temporal surface recording sites. the schizophrenic subjects were assigned to diagnostic subgroups on the basis of dsm-iii-r criteria following independent ... | 1991 | 1912124 |
| analysis of the interaction between charged side chains and the alpha-helix dipole using designed thermostable mutants of phage t4 lysozyme. | it was shown previously that the introduction of a negatively charged amino acid at the n-terminus of an alpha-helix could increase the thermostability of phage t4 lysozyme via an electrostatic interaction with the "helix dipole" [nicholson, h., becktel, w. j., & matthews, b. w. (1988) nature 336, 651-656]. the prior report focused on the two stabilizing substitutions ser 38----asp (s38d) and asn 144----asp (n144d). two additional examples of stabilizing mutants, t109d and n116d, are presented h ... | 1991 | 1911773 |
| audit of hyperthyroxinaemia and thyrotoxicosis using a sensitive tsh assay. | the aim of this study was determine (a) the causes of hyperthyroxinaemia and (b) the biochemical profile of thyrotoxicosis in a general hospital laboratory for one year using a sensitive tsh assay. total t4 (tt4) and tsh were measured in all 8,382 samples and tt3, free t4, free t3 and thyroxine binding globulin (tbg) in selected cases. tt4 was elevated in 215 (2.6%). 159 (74%) were due to thyrotoxicosis; 41 (19%) to elevated tbg and 15 (7%) non-thyroidal illness. thyrotoxicosis (serum tsh less t ... | 1991 | 1910022 |
| pregnancy-induced changes in thyroid function: role of human chorionic gonadotropin as putative regulator of maternal thyroid. | marked changes in maternal thyroid activity occur in pregnancy. it has been suggested that hcg may stimulate maternal t4 secretion, given its in vitro thyrotropic activity ascribed to a significant degree of structural homology with tsh. in a longitudinal study of 32 normal pregnant women, we attempted to clarify the functional activity of the thyroid in early and late pregnancy and the possibility of a nonpituitary control on the thyroid. total t4 and t4-binding globulin levels were increased f ... | 1991 | 1909707 |
| high levels of serum soluble interleukin-2 receptors in hyperthyroid patients: correlation with serum thyroid hormones and independence from the etiology of the hyperthyroidism. | the serum levels of soluble interleukin-2 receptors (s-il-2r) and soluble cd8 antigens (s-cd8) were measured in 33 patients with graves' disease (gd), 29 with toxic nodular goiter (tng), 6 with toxic adenoma (ta), and 12 with hypothyroidism, as well as in 11 patients with infectious mononucleosis (known to have high s-il-2r and s-cd8 levels) and 34 normal controls. serum levels of t3 and t4, both total and free, and of tsh were simultaneously determined. s-il-2r levels were significantly higher ... | 1991 | 1909702 |
| [studies on nonthyroidal illness after heart surgery]. | the concentrations of pituitary hormones (tsh and prl), thyroid hormones (free-t4 and free-t3), thyroid hormone binding protein (tbg) and lipids (tg and ffa) in the blood were measured in order to examine the physiology of nonthyroidal illnesses that occurred as a result of heart surgery as well as their effects on the pituitary and thyroid glands. the subjects of the study consisted of 30 adults with congenital and acquired heart disease. blood concentrations of tsh, prl, free-t4, free-t3, and ... | 1991 | 1909417 |
| an efficient method for constructing a chimeric bacteriophage t4 to estimate the regulatory signals. | a system for the quantitative estimation of t4 regulatory signals in bacteriophage t4 was developed. we constructed a transmitter-reporter [corrected] plasmid vector, pcv22, which is able not only to fuse transcriptional and translational signals to the coding region of lacz gene (reporter) but also to transmit the fused gene into t4 phage genome (transmitter). the regulatory signals of t4 phage genes fused with the reporter gene was transmitted efficiently into t4 phage (t4dc-lac phage; a recei ... | 1991 | 1909125 |
| carcinoma of the tonsillar fossa: a nonrandomized comparison of irradiation alone or combined with surgery: long-term results. | the results of therapy are reported in 296 patients with histologically proven epidermoid carcinoma of the tonsillar fossa; 127 were treated with irradiation alone (5,500 to 7,000 cgy), 133 with preoperative radiotherapy (2,000 to 3,000 cgy) or were initially planned for preoperative irradiation but treated with radiotherapy alone, and 36 with postoperative irradiation (5,000 to 6,000 cgy). the operation in all but 4 patients consisted of an en bloc radical tonsillectomy with ipsilateral lymph n ... | 1991 | 1907952 |
| carbaryl-induced thyroid dysfunction in the freshwater catfish clarias batrachus. | freshwater female catfish, clarias batrachus, were exposed to sublethal concentrations of carbaryl (12 mg/liter for 96 hr and 5 mg/liter for 16 days) during the prespawning and spawning phases of their annual reproductive cycle. the effects on the levels of thyroxine (t4) and triiodothyronine (t3), the t3/t4 ratio, and the extrathyroidal conversion of t4 to t3 were studied in serum, pharyngeal thyroid, and anterior and posterior kidney. carbaryl exposure for 96 hr suppressed t4 but elevated t3 a ... | 1991 | 1907904 |
| effects of hypophysectomy on pro-thyrotropin-releasing hormone concentrations in rats. | the effects of hypophysectomy on pro-thyrotropin-releasing hormone (pro-trh) and trh concentrations in the rat hypothalamus, cerebrum, cerebellum and brain stem, stomach and eye were studied. the hypophysectomy was performed via ear route and the rats were used for experiments at seven days after the operation. the hypophysectomized rats were administered t4 (500 micrograms/kg), t3 (100 micrograms/kg), trh (1.0 mg/kg), or bovine tsh (1.25 iu/kg) was injected ip, and five rats in each subgroup we ... | 1991 | 1907564 |
| [evaluating thyroid gland function in patients with protein anomalies]. | the euthyroid hyperthyroxinemia (eht) is characterized on the one hand by a normal basal ths or trh-tsh response but also by high plasma values of total thyroxine (tt4) on the other. however if only tt4 is assessed, "hyperthyroidism" may be diagnosed erroneously. eht may be caused by an increase of specific thyroxine binding proteins which may be hereditary (permanent) or acquired (transient). the most frequent disturbance is due to an estrogen induced increase of thyroxine binding globulin (tbg ... | 1991 | 1907423 |
| nucleotide deletion resulting in frameshift as a possible cause of complete thyroxine-binding globulin deficiency in six japanese families. | complete t4-binding globulin deficiency (tbg-cd) is inherited in an x-linked fashion. a nucleotide substitution has been shown to cause this hereditary condition in caucasians of french canadian origin. heterogeneity in molecular mechanisms for tbg-cd has also been reported. genomic dna from a japanese male exhibiting tbg-cd was subjected to polymerase chain reaction, and the generated dna fragments were sequenced. a single nucleotide deletion was found in the first base of the codon for amino a ... | 1991 | 1906892 |
| heterologous in vitro transcription from two archaebacterial promoters. | a cell-free extract of sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of halobacterium halobium and at the promoter for the transcript t4 of the temperate h. halobium phage phi h. the corresponding promoter from the virulent phage mutant phi hl1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression. | 1991 | 1906812 |
| comparative survey of blood thyroxine binding proteins in turtles. | the nature of plasma thyroxine (t4) binding activity was surveyed in turtles; binding to [125i]t4 was measured on polyacrylamide gel electrophoresis--page--and on minicolumns of sephadex g-25. an electrophoretically distinct t4 binding protein was identified in all 8 species of pseudemys studied and in 3 other genera (chrysemys, deirochelys, and emyoidea) of the same family, emydidae. levels of this binding activity were highly variable among individuals, but they consistently showed a similar l ... | 1991 | 1906524 |
| effects of thyroid status and fasting on hepatic metabolism of apolipoprotein a-i. | metabolism of apolipoprotein (apo)a-i was studied in normal and chow-fed hyperthyroid rats, in 24-h fasted untreated male rats, and in rats after thyroparathyroidectomy (txptx). rats were made hyperthyroid by administration of t3 (9.6 micrograms/day) or t4 (30 micrograms/day) with an alzet osmotic minipump. hyperthyroidism produced a similar two- to threefold elevation in plasma levels of apoa-i in male or female animals. during treatment with t3, plasma levels of t3 ranged from 200 to 400 ng/dl ... | 1991 | 1906085 |
| exaggerated and prolonged thyrotrophin releasing hormone (trh) test responses in tertiary hypothyroidism. | a 60 year old man with panhypopituitarism due to a large meningioma and prolonged and exaggerated thyroid stimulating hormone (tsh) responses is described. initial investigations showed a subnormal urinary free cortisol concentration, a low serum cortisol taken at 0900 hours, and a low free t4 concentration. the tsh was towards the upper end of the normal range. subsequently pituitary function tests showed subnormal production of luteinising hormone in response to luteinising hormone releasing h ... | 1991 | 1906072 |
| thyroid hormone regulates developmental changes in muscle during flounder metamorphosis. | morphological and biochemical changes in the muscular tissue of metamorphosing flounder were studied in relation to the regulatory role of thyroid hormone. premetamorphic larvae were reared in seawater alone or seawater containing either thyroxine (t4) or an antithyroid drug (thiourea, tu). histological changes in the muscle were examined and biochemical changes in the muscle proteins were evaluated by sds-page and immunoblotting for troponin t (tnt). the muscle tissue of premetamorphic larvae w ... | 1991 | 1905253 |
| thyroid vascular conductance: differential effects of elevated plasma thyrotropin (tsh) induced by treatment with thioamides or tsh-releasing hormone. | we have reported previously that thyroid gland blood flow, expressed as vascular conductance (c) per mass, is decreased at very low and increased at very high chronic plasma tsh concentrations, but is apparently unchanged over a broad range of plasma tsh concentrations encompassing normal levels. the aim of the present study was to examine the apparently very steep dose-response relationship between elevated plasma tsh and thyroid vascular c/mass. in the first series of experiments, endogenous p ... | 1991 | 1905224 |
| dna single stranded gaps formed during dna repair synthesis induced by methyl methanesulfonate are filled by sequential action of aphidicolin- and dideoxythymidine sensitive dna polymerases in hela cells. | dna repair synthesis induced by methyl methanesulfonate in preconditioned hela cells in which dna replicative synthesis had been highly suppressed was inhibited by aphidicolin (an inhibitor of dna polymerases alpha and delta) and dideoxythymidine (ddthr, an inhibitor of dna polymerase beta). incomplete repair patches sensitive to exonuclease iii were accumulated in the presence of aphidicolin while not in the presence of ddthr. these patches were comopleted by the combined action of klenow fragm ... | 1991 | 1905189 |
| prostate carcinoma--the value of t stage and grade in predicting metastases and prognosis. a cost-effective approach to clinical staging. | in order to evaluate the initial t stage and tumour grade as predictors of metastatic disease and prognosis in adenocarcinoma of the prostate, 963 patients were reviewed. of the patients, 41% presented with metastatic disease. stage t4 tumours were associated with a consistently poor prognosis, and 70% of such patients had demonstrable distant metastases. no patient with tof local disease had metastases or died of prostate cancer during follow-up. the incidence of metastases was also low in stag ... | 1991 | 1905062 |
| antiproliferative effects of natural human tumor necrosis factor-alpha, interferon-alpha, and interferon-gamma on human pancreatic carcinoma cell lines. | the antiproliferative effects of natural human tumor necrosis factor-alpha (nhutnf-alpha), natural human interferon-alpha (nhuifn-alpha), and natural human interferon-gamma (nhuifn-gamma) were investigated in human pancreatic carcinoma cell lines. hup-t3, hup-t4, and bxpc-3 carcinoma cells exhibited mild growth inhibition after treatment with nhutnf-alpha. hup-t3, hup-t4, mia-paca-2, and bxpc-3 cells exhibited mild or marked growth inhibition after treatment with nhuifn-alpha. incubation with nh ... | 1991 | 1904907 |
| an enzyme immunoassay for determining total thyroxine in human serum using an ultramicroanalytical system. | a simple, rapid competitive ultramicroelisa assay has been developed for the measurement of total thyroxine (t4) using only 10 microliters of serum. our novel ume is based on fluorescence measurements of the hydrolytic product of 4-methyl-umbelliferyl-beta-d-galactopyranoside. t4-beta-galactosidase conjugates and monoclonal antibodies were immobilized on polyvinyl plates, with sodium salicylate used as a blocking agent for thyroxine binding protein. the analytical steps were carried out using a ... | 1991 | 1904803 |
| identification of (+) and (-) anti benzo[a]pyrene dihydrodiol epoxide-nucleic acid adducts by the 32p-postlabeling assay. | purine deoxyribonucleoside 3'-phosphates were reacted with the (+)- and (-)-enantiomers of the anti dihydrodiol epoxide of benzo[a]pyrene. products from cis and trans opening of the epoxide ring were separated by hplc and they were identified by comparison of their cd spectra with those known for the corresponding nucleoside adducts. thereafter, the eight known benzo[a]pyrene-purine deoxyribonucleoside-3'-phosphate adducts were postlabeled with [32p]atp and t4 kinase and the positions of these i ... | 1991 | 1904321 |
| immunogenetic susceptibility for post-schistosomal hepatic fibrosis. | in 19 children with hepatic fibrosis as the result of continued schistosomiasis mansoni and 20 children without hepatic fibrosis, the following studies were carried out: hla antigen typing for 30 antigens, immune response of t lymphocytes to schistosome antigen by measuring dna synthesis evidenced by 3h-thymidine uptake, and measurement of total okt3+, okt4+, and okt8+ cells using monoclonal antibodies. patients with hepatic fibrosis were mostly high responders in contrast with those without fib ... | 1991 | 1904196 |
| inherited primary hypothyroidism with thyrotrophin resistance in japanese cats. | mutant cats were developed with non-goitrous primary hypothyroidism. they were clinically characterized by severely retarded growth, mild anaemia and high mortality in the young. they responded markedly to thyroid hormone replacement. thyroid glands in the mutants were normal in position but slightly reduced in size. laboratory studies revealed low serum concentrations of thyroxine (t4) and tri-iodothyronine (t3), and increased serum concentrations of tsh. administration of trh induced no furthe ... | 1991 | 1904088 |
| proteolytic processing of thyroglobulin by extracts of thyroid lysosomes. | the release of t4 and t3 from the prohormone thyroglobulin (tg) occurs in thyroid lysosomes. to examine the role of cathepsin-b, -d, and -l, the three major endopeptidases in this process, we incubated rabbit [125i]tg, labeled in vivo, with lysosomal extracts from human thyroids. iodopeptide formation was evaluated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate after short term incubations (20-45 min), while iodoamino acid release was assessed by paper chromatography after long ... | 1991 | 1903699 |
| effect of the cardiac inotropic drug, opc 8212, on pituitary-thyroid function in the rat. | 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1 piperaznyl]-2(1h)-quinolinone (opc 8212) is a new synthetic quinolinone with potent cardiac inotropic action in man. long term oral administration of opc induces goiter and thyroid tumor formation in rats, associated with decreases in serum t4 and increases in serum tsh concentrations. studies were carried out to explore the mechanisms responsible for these drug induced abnormalities. opc 8212, administered for 1 week at doses of 500 and 2000 mg/kg.day m ... | 1991 | 1903694 |
| thyroid abnormalities during lithium treatment. | thyroid function was evaluated in 150 sardinian outpatients at different stages of lithium treatment. a visible and/or palpable goitre was found in 51% of patients, and there was no apparent correlation with the duration of treatment. no cases of symptomatic hypothyroidism were observed, but subclinical hypothyroidism was present in 19% of patients. the prevalence of specific antithyroid antibodies was positively correlated with age and duration of lithium treatment, and was higher in women. sub ... | 1991 | 1903238 |
| subtle primary hypothyroidism in patients treated for acute lymphoblastic leukemia. | we evaluated serum thyroid hormone and thyroid antibody levels, the serum tsh response to trh, and the circadian pattern of serum tsh in 10 children and adolescents after radiation therapy for acute lymphoblastic leukemia. four patients had received central nervous system preventive cranial irradiation and intrathecal chemotherapy, and the remaining 6 patients were treated with craniospinal irradiation for central nervous system relapse. serum total t4 and t3 concentrations were within the norma ... | 1991 | 1903235 |
| effect of thyroid hormone on the pre- and post-natal development of the rat testis. | the relationship between thyroid function and testicular development in the rat was investigated. hypothyroidism was induced during fetal or post-natal life by adding methimazole (mmi) to the drinking water of pregnant or lactating mothers. a group of newborn rats was treated with mmi and i.p. injections of l-tri-iodothyronine (l-t3). hypothyroidism was shown by the reduced serum levels of total t3 and of total thyroxine (t4) in pregnant mothers and in pubertal rats. testes were studied using li ... | 1991 | 1903146 |
| effects of recombinant human interleukin-2 and tumor necrosis factor-alpha with or without interferon-gamma on human thyroid tissues from patients with graves' disease and from normal subjects xenografted into nude mice. | we have compared the effects of interleukin-2 (il-2) or tumor necrosis factor-alpha (tnf alpha) administration with or without interferon-gamma (ifn gamma) on graves' and normal thyroid tissue xenografts in the nude mouse (in the absence of an intact immune system) in terms of possible functional, immunological, or histological changes. the dosages of recombinant human il-2, tnf alpha, and ifn gamma given to each mouse were 250, 800, and 4000 u, respectively; they were injected ip daily for 6 co ... | 1991 | 1902846 |