Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the association of african swine fever virus with blood components of infected pigs. | the distribution of african swine fever virus (asfv) in whole blood, plasma, red blood cells (rbc) and white blood cell (wbc) sub-populations was determined in pigs infected with virulent virus. changes in the rbc and wbc populations were also examined. total wbc counts decreased and rbc numbers remained unchanged during the course of the disease. the number of circulating lymphocytes decreased whilst neutrophil numbers increased owing to the replacement of mature forms by juveniles. virus was p ... | 1977 | 563710 |
| african swine fever virus replication in porcine lymphocytes. | purified preparation of porcine lymphocytes were infected with three isolates of virulent african swine fever virus (asfv). electron microscopy showed the presence of small numbers of mature virus particles in degenerating cells. the titres of infective virus released were low and reached a maximum by 24 h after infection. | 1977 | 562925 |
| improved method for the purification of hog cholera virus grown in tissue culture. | a method for purification of hog cholera virus (hcv) is presented. cell-associated virus was extracted from pk15 cells 17 hours p.i. by fluorocarbon treatment. the virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. final purification was achieved by rate zonal centrifugation in a 7--35 per cent sucrose gradient. this procedure permits a recovery of approximately 40 per cent of viral infectivity. a specific infectivity of abo ... | 1977 | 560840 |
| hog cholera virus : sensitivity to hydrolytic enzymes. | the actions of trypsin and phospholipase c on th infectivity of hog cholera virus (hcv) were studied. inactivation kinetics reveal a marked decrease of the infectivity of hc virus in the presence of these two agents. the particular sensitivity of this virus towards proteolytic action sheds light on certain of its behavior characteristics in the field. virus infectivity seems to be dependent on the integrity of membrane phospholipids. no relation was observed between the rate of inactivation and ... | 1977 | 560170 |
| african swine fever: pathogenicity and immunogenicity of two non-haemadsorbing viruses. | the virulence of 2 non-haemadsorbing african swine fever virus isolates were compared with 2 haemodsorbing viruses. while 3 of these isolates usually produced acute death in pigs, 1 non-haemadsorbing virus caused either a fatal infection with an extended course, or few or no obvious signs of infection. pigs that survived infection with the latter virus were resistant to the lethal effects of the other 3 strains as well as to a pool of 7 isolates made from ornithodorus porcinus porcinus (senus wa ... | 1979 | 551362 |
| [viral carrier state and viral excretion in classical swine fever]. | studies on swine pest virus carrying and elimination on swine vaccinated by lapinizated vaccine strain "k" from rabbits were performed. vaccinated swine were injected with the pathogenic virus on the 60th day post vaccination and to them were added not immunized swine with the aim to discover virus elimination. the experimental swine were decapitated on the 3, 5, 6 and 7th day post infection. alternating passages were made by emulsion of the inner organs of these swine on not immunized swine as ... | 1979 | 549263 |
| an immunofluorescence neutralization test in disposable plastic trays for demonstrating classical swine fever virus antibodies. comparison of a micro with a macro method. | 1979 | 539221 | |
| [nature and specificity of the auxilliary rabbit test in the diagnosis of swine fever]. | experiments were carried out with the aim to reveal the causes for the inhibited temperature reaction in rabbits to swine pest virus strain k in case of previous injection with virulent pest virus. it was established that in the serum of rabbits injected with virulent swine pest virus specific antibodies are found, which are capable to neutralize the lappinized k virus introduced later. this phenomenon which is specific enough and highly sensitive can be used with success as an auxiliary biologi ... | 1979 | 532090 |
| cross-links in african swine fever virus dna. | african swine fever virus dna sediments in neutral sucrose density gradients as a single component with a sedimentation coefficient of 60s. in alkaline sucrose density gradients, this material shows two components with sedimentation coefficients of 85s and 95s, respectively. the sedimentation rate value of alkali-denatured virus dna in neutral sucrose density gradients and the renaturation velocity of denatured dna show that is reassociated much faster than expected from its genetic complexity. ... | 1979 | 513189 |
| [studies on the increase of weights of lymphatic glands, of lymph and peritoneal fluid and their contents of chymotrypsin and virus in pigs suffering hog cholera (author's transl)]. | swine fever is conceived as a disorder of the enzyme systems, that are controled by serine proteases. the virus is replicated in the cells of the lymphomycoid complex, whereby the production of a chymotrypsin is induced. in swine fever the lymphatic glands and the lymph flow are increased. fifteen normal pigs had chymotrypsin contents in the lymph of the body lymphnodes of 0,4 u/l, nine pigs suffering hog cholera 1,5 u/l. in the intestinal lymphnodes the chymotrypsin concentration was normally 2 ... | 1979 | 506545 |
| the replication of virulent and attenuated strains of african swine fever virus in porcine macrophages. | the replication of virulent and attenuated strains of african swine fever virus (asfv) was studied in pure cultures of swine macrophages. to ensure complete destruction of the macrophage monolayers about 50--100 times more virulent asfv was needed than attenuated virus although both isolates could be used to establish persistently infected cultures. interferon did not appear to influence virus yields from such cultures. fluorescent and electron microscopy studies of infected macrophages suggeste ... | 1979 | 496644 |
| solid-phase radioimmunoassay techniques for the detection of african swine fever antigen and antibody. | a solid phase radioimmunoassay (ria) has been successfully developed to measure both african swine fever virus (asfv) antigen and antibody. studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50--500 had50/ml. both direct and indirect antibody ria have been developed and have proved to be approximately 100 times fore sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophor ... | 1979 | 489964 |
| thermal inactivation of hog cholera virus in ham. | experiments were conducted to determine the temperatures required to inactivate hog cholera virus (hcv) in fresh ham after 1 minute and in cured and processed (canned) ham after 90 minutes. a momentary or "flash" temperature of 71 c for 1 minute caused inactivation of the virus in 15 of 15 cubes (2 cm3) of ham. hog cholera virus was destroyed in 21 of 21 canned hams (weighing 0.91 kg each) when an internal temperature of 65 c was sustained for 90 minutes. pigs were found to be more sensitive tha ... | 1979 | 475124 |
| [comparative precipitation studies between the highly-virulent virus of european swine fever, swine-fever-virus split products and precipitating swine-fever antigens from the organs of swine-fever infected pigs]. | 1977 | 414484 | |
| [diaplacental transfer of nonvirulent tvm-1 hog-cholera virus]. | 1977 | 406714 | |
| requirement of cell nucleus for african swine fever virus replication in vero cells. | the role of the cell nucleus in the development of african swine fever virus in vero cells has been studied. no viral growth could be detected in enucleated cells under conditions that allow normal development of sindbis virus. furthermore, african swine fever virus dna synthesis was inhibited more than 95% after infection of enucleated vero cells as compared with normal cells. | 1977 | 403300 |
| a solid-phase enzyme linked immunosorbent assay for the detection of african swine fever virus antigen and antibody. | a solid-phase enzyme-linked immunosorbent assay was developed to measure both african swine fever virus (asfv) antigen and antibody. experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50--500 had50/ml. the assay was more sensitive than those used at present to detect asfv antibody and it is suggested that it might be of great diagnostic use in countries where african swine fever has recently appeared. | 1979 | 385771 |
| effect of disodium phosphonoacetate and iododeoxyuridine on the multiplication of african swine fever virus in vitro. | disodium phosphonoacetate (paa) was found to inhibit the replication of african swine fever virus (asfv). the action of this compound has been compared with the inhibitory capacity of iododeoxyuridine (idu) upon asfv growing in vero cells. the study was done by the immunofluorescence technique in order to detect formations of cytoplasmic virus antigens and inclusion bodies; both were found to be inhibited by idu and paa. at 100 microgram/ml, idu blocked completely the multiplication of asfv and ... | 1979 | 378573 |
| [isolation of a cytolytic strain of hog cholera virus from ib-rs2 cells (author's transl)]. | an infectious agent, able to induce a definite cytopathic effect in pig kidney cell monolayers, was isolated from the ib-rs2 cell line. immunofluorescence, seroneutralization and ultracentrifugation studies have permitted to identify this agent as hog cholera virus (hcv). representative hcv-strains being devoid of pathogenicity in tissue culture, the practical and theoretical interest of such an isolation is discussed. | 1978 | 358888 |
| african swine fever: microplaque assay by an immunoperoxidase method. | a microplaque assay for vero cell-adapted lisborn '60 strain of african swine fever virus (l'60-uncloned) and a large plaque-forming strain cloned from the l'60-uncloned strain was developed by an immunoperoxidase method. the immunoperoxidase method can be used to stain microplaques of 3 days after inoculation, whereas the conventional plaque assay requires 5 to 7 days to develop visible plaques. a linear relationship between viral concentration in the inoculum and plaque numbers was observed. v ... | 1978 | 345892 |
| the growth of virulent african swine fever virus in pig monocytes and macrophages. | the replication of virulent african swine fever virus (asfv) in cultures of monocytes and macrophages derived from pig bone marrow (pbm) and pig leukocyte (pl) cells was investigated by light microscopy, immunofluorescence, haemadsorption and infective virus release. monocytes showed a high rate of infection and complete destruction within 2 to 3 days, whereas macrophages had only a very low level of infection and survived to form persistently infected cultures. these observations may explain th ... | 1978 | 340610 |
| additional characteristics of disease caused by the african swine fever viruses isolated from brazil and the dominican republic. | 1979 | 298918 | |
| some characteristics of african swine fever viruses isolated from brazil and the dominican republic. | 1978 | 287093 | |
| transmission of hog hog cholera virus by mosquitoes. | mosquitoes trapped during an epizootic of hog cholera (hc) in maryland in 1969 were prepared into 40 pools which were inoculated in pigs. hog cholera virus was confirmed in pigs inoculated with 8 of 40 pools of mosquitoes. generally, the pigs contracting hc developed chronic infections with persistent viremia that lasted 30 or more days. two pigs seemed healthy when euthatized 62 and 80 days after inoculation, yet viremia of high titer was detected in each. experimental studies were performed wi ... | 1975 | 237444 |
| nonarbo-togaviridae: comparative hydrodynamic properties of the pestivirus genus. brief report. | the sedimentation coefficient and buoyant density of hog cholera, bovine viral diarrhea and border disease viruses, have been compared with those of representative members of the family togaviridae. it appears that the pestivirus genus is a homogeneous group which is not only antigenically but also structurally, unrelated to the other genera of the togavirus family. | 1979 | 232414 |
| detection of three polypeptides in preparations of bovine viral diarrhoea virus. | radiolabelled bovine viral diarrhoea/mucosal disease virus (bvdv) strains nadl and oregon c24v were purified by different steps. following immunoprecipitation, electrophoresis in sds-polyacrylamide gels revealed three bvdv structural polypeptides with molecular weights of 57 (vp1), 44 (vp2), and 34 (vp3) kd. the two larger bvdv polypeptides vp1 and vp2 were found to be glycosylated (gp57, gp44). the data obtained on bvdv structural proteins demonstrate common features with hog cholera virus and ... | 1979 | 222243 |
| [reverse plaque formation by hog cholera virus inducing interference with vsv (author's transl)]. | infection of pk15 cells with various strains of hog cholera (hcv, togaviridae) induces a transient refractory state to vsv. the reverse plaque procedure is convenient for hcv titration of virulent, "chronic" and attenuated strains. | 1978 | 205199 |
| differences in pathogenicity and antigenicity among hog cholera virus strains. | using antiserum against a particular strain of bovine viral diarrhea virus, the strains of hog cholera virus were divided into two groups, h and b, on the basis of the difference in the degree of neutralization. group h consisted of strains reacting poorly in neutralization, and group b consisted of strains reacting well with bovine viral diarrhea antiserum. most of the strains of group h induced a typical clinical form of hog cholera in experimentally infected pigs. inoculation of pigs with a s ... | 1977 | 203871 |
| [the immunity against challenge with swine fever virus of piglets from sows vaccinated with c-strain virus (author's transl)]. | the protective value of maternal antibodies against exposure by contact to virulent swine fever virus was investigated in a group of 40 piglets at the age of 33-40 days. the piglets were from a single farm and derived from 10 sows which had been vaccinated once with c-strain virus 5.5 months before farrowing. four contact controls of approximately the same age as the test-groups and born from non-vaccinated sows died 4-10 days (av. 7.5 days) after the onset of symptoms. three pigs from the group ... | 1977 | 199961 |
| tween 80: a marker for differentiation of hog cholera and bovine viral diarrhea viruses. | markers for differentiating hog cholera and bovine viral diarrhea viruses were studied using tween 80, chloroform, trichlorotrifluoroethane and tri (n-butyl) phosphate. attenuated a and virulent ames strains of hog cholera virus were employed. moreover, the nadl pk-15 cell culture adopted strain and low cell culture passaged purdue strain of bovine viral diarrhea virus were used. these viruses were reacted with 2,500 micrograms/ml of tween 80 for one hour at 37 degrees c. when attenuated a and v ... | 1977 | 188531 |
| swine buffy coat culture: an aid to the laboratory diagnosis of hog cholera. | a 2-step technique for the isolation of hog cholera (hc) virus consisting of an initial culture on buffy coat (bc) cultures and subinoculation to a pig kidney cell line (pk-15) was described. by this technique, hc virus was confirmed in specimens from 65 herds in which the conventional cell culture isolation technique failed. the herds were located in 20 states and puerto rico. specimens from 29 of the 65 herds were inoculated into specific-pathogen-free (spf) pigs. hog cholera virus was recover ... | 1975 | 163064 |
| the structural components of hog cholera virus. | hog cholera virus grown in pk-15 cells and sk-cells was labeled with [35s]methionine and [3h]uridine. at least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. the molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). the molecular weight of the viral rna was determined to be about 4 x 10(6) in polyacrylamide-agarose-gel electrophoresis. in sucrose gradients the r ... | 1977 | 141817 |
| synthesis of dna in cells infected with african swine fever virus. | incorporation of 14c-thymidine by cells infected with african swine fever virus (asfv) occurs in the nucleus. part of this dna is transferred to the cytoplasm and becomes resistant to dnase. the nuclear fraction washed with triton x100 retained all labeled dna and was able to synthesize viral and cellular dna under in vitro conditions in the presence of the four deoxynucleoside triphosphates, mg+2, and sucrose. under similar conditions nuclei from uninfected cells synthesized very little dna. | 1979 | 117788 |
| electron microscopic observation of african swine fever virus development in vero cells. | african swine fever virus emerges from infected vero cells either from areas of smooth cell surface or from microvilli. the two patterns may occur at different sites on the same cell and are unique for this virus. the scanning electron micrographs supplement regular thin section views. | 1978 | 99488 |
| properties of border disease virus as studied in a sheep cell line. | a fetal lamb muscle cell line has been isolated in which two strains of border disease virus replicate well and induce a clear-cut cytopathic effect, thus providing a sensitive and practical assay system for both the virus and neutralizing antibodies. this system enabled us to study some characteristics of border disease virus in comparison with two other agents responsible for hog cholera and bovine viral diarrhea. our results indicate that the last virus and the border disease agent are indist ... | 1979 | 94539 |
| [control of immunity against swine plague by the viral neutralization test on rabbits]. | an idea may be got on the properties of the vaccine by applying a specific gamma-globulin against swine fever in pigs, mixed in definite amounts with lapinized k-vaccine and this mixture is injected to rabbits. by studying the mixture of sera from pigs, vaccinated against swine fever prior to a fixed period of time, and the k-vaccine, applied to rabbits, an idea may be obtained on the tension of the immunity and re-vaccination requirements may be assessed. | 1978 | 87059 |
| a direct plaque assay for hog cholera virus. | direct plaque formation with representative strains of hog cholera virus (hcv) has been obtained using several pig kidney cell lines under agar overlay. hcv-infected cells appear as hazy plaques when viewed against an indirect light source, and as white plaques after neutral red staining. hcv assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique. | 1978 | 80444 |
| negative staining of a non-haemadsorbing strain of african swine fever virus. | since the application of negative staining, preceded by fixation, prevents the disruption and distortion of the capsid of the african swine fever virus, improved contrast and evaluation of the appearance and size of virus particles in the electron microscope is possible and, in addition, the icosahedral shape of the virus is demonstrable. the mature virus particle contains at least 2 capsid layers and an outer envelope. | 1977 | 78480 |
| reverse plaque formation by hog cholera virus of the gpe-strain inducing heterologous interference. | a simple and rapid plaque procedure was developed for the assay of hog cholera virus (hcv) of a particular strain, gpe-, based on its intrinsic interference with vesicular stomatitis virus (vsv) on the primary swine testicle cells and on an established swine kidney cell line; the procedure is called the reverse plaque formation (rpf) method. the plaques were produced as colonies of hcv-infected cells which were vsv-sensitive, disintegrated cell sheet. these plaques became visible after 15 to 20 ... | 1976 | 61176 |
| [effect of passive immunity on subsequent vaccination against swine fever]. | studied was the effect of a serum paratyph and aujeszky gammaglobulin injected 10, 15, and 20 days prior to the crystal-violet and the lapinized vaccine. if the animals were infected with a pathogenic swine fever virus 10 to 15 days after their treatment with serum at the rate of 0.5 cu. cm per kg, part of them died, and the remaining contracted the disease showing a protracted course. the control vaccination on the 20th day after the animals were injected with serum led to the death of all of t ... | 1975 | 53938 |
| quantitative and rapid assays of togaviruses by immunofluorescence. | for the quantitative assay of selected togaviruses, suspensions of bhk cells were inoculated with virus and grown in spinner cultures. at intervals, dependent on the growth characteristics of the viruses, about 10(3) cells were centrifuged on to microscope slides and then stained with fluorescent antibody. for rapid demonstration (2--3 hr) of specific viral antigens, virus was bound in successive dilutions onto microscope slides and stained. binding of specific anti-virus antibodies in both meth ... | 1979 | 40420 |
| isoelectric focusing of hog cholera virus. | the isoelectric point of hog cholera virus (alfort strain) has been determined (phi = 4.8). a clear stimulation of its infectivity can be recorded at this ph. the isoelectrophoretic profiles of strains isolated in the field show no significant differences. | 1977 | 18081 |