Publications

ascariasis is a zoonosis in denmark.	a preliminary epidemiological survey indicated an association between ascaris infections in danish patients and contact with pigs or pig manure. in the present study, we compared ascaris worms collected from humans and ascaris worms collected from pigs by amplified fragment length polymorphism (aflp) analysis, a technique for whole-genome fingerprinting, and by pcr-linked restricted fragment length polymorphism (pcr-rflp) analysis of the internal transcribed spacer region of nuclear rdna. the af ...	2005	15750075
comparison of a commercial elisa with the modified agglutination test for detection of toxoplasma infection in the domestic pig.	the modified agglutination test (mat) and a commercially available enzyme-linked immunosorbent assay (elisa) were compared for detection of antibodies to toxoplasma gondii in naturally-infected market-aged pigs. infected pigs were obtained from commercial slaughter facilities and from farms where infection had previously been detected. infection was confirmed by bioassay in cats. for 70 bioassay positive pigs, 60 were positive by mat (85.7% sensitivity) and 62 were positive by elisa (88.6% sensi ...	2005	15740853
first record of trichinella pseudospiralis in the slovak republic found in domestic focus.	infection of trichinella spp. is widespread among wildlife in slovakia and the red fox (vulpes vulpes) is the main reservoir of trichinella britovi. trichinella spiralis has been rarely documented in sylvatic and domestic animals of this country. during routine examination of domestic pigs at the slaughter, trichinella larvae were detected by artificial digestion in a domestic pig of a large-scale breeding farm in eastern slovakia. the parasite has been identified by molecular (pcr) and biochemi ...	2004	15725537
transepicardial autologous bone marrow-derived mononuclear cell therapy in a porcine model of chronically infarcted myocardium.	cell therapy is becoming a viable strategy to improve revascularization and myocardial function after myocardial injury. we evaluated the effect of bone marrow-derived mononuclear cell (bmmnc) transplantation on collateral vessel development and myocardial function in a porcine model of chronically infarcted heart.	2004	15721847
seroprevalence of noroviruses in swine.	noroviruses (nvs) are important human pathogens that cause acute gastroenteritis. genetically related animal enteric nvs have also been described, but there is no evidence of interspecies transmission of nvs. in this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of two human nvs (norwalk and hawaii) and one swine nv (sw918) that is genetically related to gii human nvs. recombinant sw918 capsid protein expressed in baculovirus self-assembled i ...	2005	15695660
comparative analysis of protein coding sequences from human, mouse and the domesticated pig.	the availability of abundant sequence data from key model organisms has made large scale studies of molecular evolution an exciting possibility. here we use full length cdna alignments comprising more than 700,000 nucleotides from human, mouse, pig and the japanese pufferfish fugu rubrices in order to investigate 1) the relationships between three major lineages of mammals: rodents, artiodactyls and primates, and 2) the rate of evolution and the occurrence of positive darwinian selection using c ...	2005	15679890
the phylogeny of chinese indigenous pig breeds inferred from microsatellite markers.	a genetic study of 32 local chinese, three foreign pig breeds [duroc (du), landrace and yorkshire], and two types of wild boar (hainan and dongbei wild boar) based on 34 microsatellite loci was carried out to clarify the phylogeny of chinese indigenous pig breeds. the allele frequencies, effective numbers of alleles, and the average heterozygosity within populations were calculated. the results showed that the genetic variability of the lingao pig was the largest, while the jiaxing pig was the l ...	2005	15670125
comparison of erbium:yttrium-aluminum-garnet and holmium:yttrium-aluminum-garnet lasers for incision of urethra and bladder neck in an in vivo porcine model.	to evaluate, in an animal model, differences in wound healing and scar formation in healthy urethra and bladder neck incised with the erbium (er):yttrium-aluminum-garnet (yag) and holmium (ho):yag lasers.	2005	15667902
volumetric cine imaging for cardiovascular circulation using prototype 256-detector row computed tomography scanner (4-dimensional computed tomography): a preliminary study with a porcine model.	this is a preliminary demonstration of volumetric cine imaging of cardiovascular circulation in domestic pigs using a prototype 256-detector row computed tomography (ct) scanner. the scan range is approximately 120 mm in the craniocaudal direction, with a 0.5-mm slice thickness. the thin sections can be used to create cine loops in multiple planes. thus, the 256-detector row ct scanner overcomes some of the limitations of present helical ct methods.	2005	15665678
il-1beta in bronchial lavage fluid is a non-invasive marker that predicts the viability of the pulmonary graft from the non-heart-beating donor.	viability testing of the pulmonary graft retrieved from the non-heart-beating donor (nhbd) is mandatory for successful outcome after lung transplantation. functional assessment by ex vivo reperfusion, however, remains a cumbersome procedure. in this study, therefore, we wanted to investigate the possible value of the proinflammatory cytokines interleukin-1beta (il-1beta) and tumor necrosis factor-alpha (tnf-alpha) measured in bronchial lavage fluid (blf) in predicting functional outcome of the p ...	2005	15653374
degenerative changes of porcine intervertebral disc induced by vertebral endplate injuries.	graded endplate injuries were performed in porcine lumbar discs. the effects of such injuries were compared to control animals in which a sham operation was performed.	2005	15644752
[studies on the karyotype and bands of hybrid pig].	using usual method, we got karyotype of hybrid pig (wild soar (male symbol) x domestic pig (female symbol)), c-band and approximate 174 bands of g-band, and we also obtained approximate 258 bands of high resolution g-band by micro-colchicin method. the result indicate that the diploid chromosome number is 2n=38; there is polymorphism in c-band,and compared with domestic pig in g-band and high resolution g-band there is no distinguish difference. they belong to the same seed.	2003	15639955
[analysis of presence and difference of sequence of porcine endogenous retrovirus in dna genomes from peripheral blood white cells of pig and mrna in tissues from mini-pigs].	the aim is to investigate biological features of porcine endogenous retrovirus (perv) of pigs in china and to provide basic parameters for evaluation of biological safety of xenotransplantation from pig to human. in this study, basic biologic features of perv of dna of peripheral blood white cells of 12 species of domestic pigs in china were examined by polymerase chain reaction; analysis of difference of perv gene sequence was investigated by sliver-stained single stranded conformational polymo ...	2004	15636362
spatial and temporal patterns of human african trypanosomosis (hat) transmission risk in the bipindi focus, in the forest zone of southern cameroon.	vector control is an effective and cost-efficient way to disrupt the transmission of human african trypanosomosis (hat); it has nonetheless been little used to date in the disease's foci. with the aim to target trapping more precisely and to develop an optimized vector control system, a transmission risk index was used in the hat focus of bipindi, in the forest zone of southern cameroon. the authors used a simplified version of the index originally developed by laveissière et al. in 1994. the ca ...	2004	15631068
long-term monitoring of classical swine fever in wild boar (sus scrofa sp.) using serological data.	in the european community, epizootics of classical swine fever (csf) in the wild boar (sus scrofa) are compulsorily monitored because transmission may occur between wild boars and domestic pigs, causing heavy economic losses to the pork industry. the estimation of incidence in populations of wild boars is generally based on viroprevalence. however, viral isolation becomes rare when the incidence is low because the virus cannot be detected for more than a few weeks following infection. on the con ...	2005	15610721
clinical aspects of percutaneous poisoning by the chemical warfare agent vx: effects of application site and decontamination.	o-ethyl s-(2-diisopropylaminoethyl) methylphosphonothioate (vx) is an extremely toxic organophosphate nerve agent that has been weaponized and stockpiled in a number of different countries, and it has been used in recent terrorist events. it differs from other well-known organophosphate nerve agents in that its primary use is as a contact poison rather than as an inhalation hazard. for this reason, we examined the effects of application site and skin decontamination on vx toxicity in anesthetize ...	2004	15605929
transfemoral catheterization of carotid arteries with real-time mr imaging guidance in pigs.	all procedures and protocols were approved by the institutional animal care and use committee of columbia university. to determine whether transfemoral catheterization of the carotid arteries can be performed entirely with real-time magnetic resonance (mr) imaging guidance, the authors catheterized the carotid arteries in six domestic pigs by using active-tracking catheters and guidewires and mr tracking software created for neurovascular procedures. the carotid arteries were successfully cathet ...	2004	15591433
feasibility of stent placement in carotid arteries with real-time mr imaging guidance in pigs.	all examinations were performed with approval from the institutional animal care and use committee of columbia university. to assess the feasibility of real-time magnetic resonance (mr) imaging-guided neurovascular intervention in a swine model, the authors placed stents in the carotid arteries of five domestic pigs. seven-french vascular sheaths were placed in the target carotid arteries via femoral access by using active mr tracking. ten nitinol stents (8-10 x 20-40 mm) were successfully deplo ...	2004	15591432
differential effect of experimental hypertension and hypercholesterolemia on adventitial remodeling.	intima-media remodeling, as frequently assessed by changes in the external elastic lamina-to-lumen area (eella), is well-described in coronary artery disease in contrast to adventitial remodeling, especially in the early disease stage.	2004	15591225
radio-frequency tissue ablation in liver trauma: an experimental study.	the liver is the most frequently injured intra-abdominal organ. radio-frequency tissue ablation (rfa) with cooled tip electrodes is here experimentally used for the treatment of liver trauma. a grade iii and a grade iii to iv trauma each were produced in the livers of 10 domestic pigs. rfa was applied around the sites of injury until hemostasis was achieved. the animals were sacrificed at 0, 3, 7, 14, and 21 days and examined. the livers were subjected to histologic and radiologic examination. t ...	2004	15586512
expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)--an immunohistochemical analysis.	this animal study (domestic pig) examined the bone formation after filling defined defects with autogenous bone or a collagen lyophilisat in combination with platelet-rich-plasma (prp) by evaluating bone matrix proteins. six groups, both materials with and without prp in two concentrations (+ 1, + 2) were compared to untreated bone by means of immunohistochemistry at 2, 4, 12 and 26 weeks. bmp-2 expression was increased at 2 weeks in the collagen + 1 group and after 4 weeks in the collagen + 1 a ...	2005	15585260
[prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs: an epidemiological investigation in shandong province].	to investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (hfrsv) infection in domestic pigs in shandong province, and study the role of domestic pigs in the prevalence of hfrs.	2004	15567780
lung biopsy with a 12-gauge cutting needle is possible using an insertion sheath in animal models.	the volume of lung tumor core biopsy specimens has been restricted because of concerns for complications such as bleeding and air leakage. in this animal experiment, we investigated the possibility of larger bore biopsies through the peripheral lung parenchyma.	2004	15561575
renal tolerance to prolonged warm ischemia time in a laparoscopic versus open surgery porcine model.	to our knowledge the effects of renal warm ischemia (wi) during laparoscopic vs open surgery have not been investigated. decreased renal blood flow during pneumoperitoneum may precondition the kidney to tolerate longer wi time. traditionally 30 minutes has defined the limit of renal wi time in open surgery. however, recent reports show renal function recovery at wi times of 45 to 120 minutes. we assessed renal function recovery after prolonged wi during laparoscopic vs open surgery in a solitary ...	2004	15538293
routes of transmission of swine hepatitis e virus in pigs.	hepatitis e virus (hev) is believed to be transmitted by the fecal-oral route in pigs. to date, in experiments, hev has been transmitted successfully only by the intravenous or intrahepatic route. to assess the route of hev transmission, 27 pigs were separated into nine groups of three pigs. positive-control pigs were inoculated intravenously with swine hev and served as the source of hev for the other groups. uninoculated contact pigs were placed in the positive-control group. on three consecut ...	2004	15528694
comparative map between the domestic pig and dog.	cross-species chromosome painting with probes derived from flow-sorted dog and human chromosomes was used to construct a high-resolution comparative map for the pig. in total 98 conserved autosomal segments between pig and dog were detected by probes specific for the 38 autosomes and x chromosome of the dog. further integration of our results with the published human--dog and cat--dog comparative maps, and with data from comparative gene mapping, increases the resolution of the current pig--huma ...	2004	15520883
inhibitory g protein overexpression provides physiologically relevant heart rate control in persistent atrial fibrillation.	the need for new treatment strategies for cardiac arrhythmias has motivated our continuing development of gene therapeutic options. previously, we reported a decreased heart rate in an acute model of atrial fibrillation after atrioventricular nodal gene transfer. here, we expand those observations to persistent atrial fibrillation and severe heart failure.	2004	15505099
co-circulation of two genetically distinct viruses in an outbreak of african swine fever in mozambique: no evidence for individual co-infection.	in 1998, domestic pigs originating from villages within a 40 km radius of ulongwe in the northern tete province of mozambique were held in a quarantine facility for a 3-month period prior to their importation into south africa. eight of a total of 25 pigs died within the first 3 weeks of quarantine of what appeared clinically and on post mortem examination to be african swine fever (asf). organs were collected and preserved in formol-glycerosaline and the presence of asf virus in these specimens ...	2004	15504588
fluid resuscitation from severe hemorrhagic shock using diaspirin cross-linked hemoglobin fails to improve pancreatic and renal perfusion.	fluid resuscitation from hemorrhagic shock is intended to abolish microcirculatory disorders and to restore adequate tissue oxygenation. diaspirin cross-linked hemoglobin (dclhb) is a hemoglobin-based oxygen carrier (hboc) with vasoconstrictive properties. therefore, fluid resuscitation from severe hemorrhagic shock using dclhb was expected to improve perfusion pressure and tissue perfusion of kidneys and pancreas.	2004	15504197
pathological changes in sarcocystis infection in domestic pigs (sus scrofa).		2004	15501157
diurnal rhythms of pinealocyte ultrastructure, pineal serotonin content and plasma melatonin level in the domestic pig.	the study was conducted to investigate diurnal changes in pinealocyte ultrastructure, pineal serotonin content and plasma melatonin concentration in the domestic pig. the immature pigs (n=24) were kept under a cycle of 12 h light : 12 h dark, with a photophase between 0800 and 2000. during the photophase the animals were exposed to direct sunlight. after four weeks the gilts were slaughtered at 0900, 1400, 2100 and 0200. the pineals were removed and divided into two parts - one for quantitative ...	2004	15493576
only transient increase of vascular growth factors and microvascular density after percutaneous myocardial laser.	we tested the hypothesis that percutaneous myocardial laser may stimulate microvascular growth in areas surrounding the laser channels.	2004	15492594
[analysis of gene expression profiles in heart tissues from two breeds of pigs].	to study the expression profiles in the heart tissues of domestic pigs,five un-normalized cdna libraries from danish adult female landrace heart, biceps femoris, aorta, left ventricle tissues and chinese adult female erhualian heart tissue were constructed. totally 35 180 expressed sequence tags (ests) were randomly sequenced. using functional clustering and statistic methods, three gene clusters and 47 genes highly expressed in landrace heart tissue were found. these genes are corresponding to ...	2004	15490873
trichinella pseudospiralis foci in sweden.	in sweden, the prevalence of trichinella infection in domestic pigs has greatly decreased since the 1970s, with no reports in the past 4 years. however, infected wild animals continue to be found. the objective of the present study was to identify the species of trichinella present in animals of sweden, so as to contribute to the knowledge on the distribution area and hosts useful for the prevention and control of this zoonosis. in the period 1985-2003, trichinella larvae were detected in the mu ...	2004	15482889
a novel intestinal anastomotic device in a porcine model.	the purpose of this study was to evaluate a novel, intraluminally deployed anastomotic device (ad). a survival study was conducted in 18 farm pigs. one early subject was excluded and replaced due to premature expiration. six animals were placed in 1 of 3 cohorts, with euthanasia and ad explantation planned at 2, 4, and 6 weeks. a distal small intestinal side-side [functional end-end] anastomosis using the ad was performed via midline laparotomy. fluoroscopy with double-contrast dilute barium and ...	2004	15481291
phenotypic features of the domestic pigs bred in the roman settlements of pompeii and caralis.	a reconstruction of the morphological features of domestic pigs from two roman settlements is here suggested by means of the study of skeletal and dental remains, with the aim at evaluating their degree of selection in comparison with wild boars. material was formed by 111 bone and tooth fragments and was uncovered during the excavations of polybius' house in pompeii and of roman buildings in the neighbourhood of caralis harbour (sardinia). the remains underwent morphological examination. the er ...	2004	15481159
molecular investigation of hepatitis e virus infection in domestic and miniature pigs used for medical experiments.	hepatitis e virus (hev) infection is highly prevalent among domestic pigs in japan. it has been reported that pig handlers such as farmers and veterinarians are at increased risk of contracting hev infection. pigs are regarded as the most acceptable candidate animals for xenotransplantation and, recently, they are being used as experimental animals.	2004	15479459
cyclooxygenase-2 expression in experimental post-transplant obliterative bronchiolitis.	epithelial cell injury, inflammation, progressive fibrosis, and airway obliteration are histological features of post-transplant obliterative bronchiolitis (ob). cyclooxygenase (cox)-2 is expressed in acute and chronic inflammatory responses. our aim was to elucidate the possible role of cox-2 in post-transplant ob by using a heterotopic bronchial porcine model. bronchial allografts from non-related donors were transplanted subcutaneously into 24 random-bred domestic pigs, each weighing about 20 ...	2004	15476277
intraocular lenses, bacterial adhesion and endophthalmitis prevention: a review.	postoperative endophthalmitis following intraocular lens (iol) implantation is still one of the most feared complications of cataract surgery. bacterial adhesion to iols during their insertion is a prominent etiological factor. polypropylene was the first biomaterial that allowed this relation of cause and effect to be proven. following adhesion, bacteria replicate, congregate and form multiple layers of microcolonies which actually represent the basic structural unit of the biofilm. the bacteri ...	2004	15472388
measurement of portal blood and transjugular intrahepatic portosystemic shunt flow with use of a retrograde thermodilutional catheter.	direct blood flow measurement in the portal vein (pv) and in transjugular intrahepatic portosystemic shunts (tips) holds potential as a means of optimizing shunt creation and predicting outcomes. a retrograde thermodilutional flow measurement catheter was recently designed. the purpose of this study was to validate and optimize the technique of blood flow measurement in the pv with use of this catheter in a swine model of tips.	2004	15466797
attempts to reproduce vacuolar myelinopathy in domestic swine and chickens.	avian vacuolar myelinopathy (avm) was first recognized as a cause of bald eagle (haliaeetus leucocephalus) mortality in 1994 in arkansas (usa) and has since caused over 90 bald eagle and numerous american coot (fulica americana) mortalities in five southeastern states. the cause of avm remains undetermined but is suspected to be a biotoxin. naturally occurring avm has been limited to wild waterbirds, raptors, and one species of shorebird, and has been reproduced experimentally in red-tailed hawk ...	2004	15465715
classical swine fever virus strain 'c'. how long is it detectable after oral vaccination?	to determine the persistence period of c-strain vaccine virus in immunized animals, domestic pigs and wild boars were vaccinated orally and killed on different days post vaccinationem (dpv). tissue samples were taken at necropsy from both species for detection of c-strain virus. from domestic pigs nasal swabs and faeces were also collected. during the investigation period (2-12 dpv) vaccine virus could never be detected in nasal secretions and in faeces of vaccinated domestic pigs. in contrast, ...	2004	15458487
effects of hypertonic versus isotonic infusion therapy on regional cerebral blood flow after experimental cardiac arrest cardiopulmonary resuscitation in pigs.	to evaluate the effects of hypertonic, isooncotic, and isotonic infusion therapy on cerebral blood flow (cbf) during and after cardiopulmonary resuscitation (cpr) from experimental cardiac arrest (ca).	2004	15451589
laser-assisted vascular end to end anastomosis of elastin heterograft to carotid artery with an albumin stent: a preliminary in vivo study.	laser-assisted end to end vascular anastomosis of an elastin heterograft to native artery may prevent problems associated with currently available vascular synthetic grafts and conventional suture anastomosis.	2004	15389735
investigations into growth and differentiation in the cartilage of the condylar process in the domestic pig. a quantitative study of endochondral cartilage growth and cell distribution.	the aim of this study was to examine the extent to which the ratio of the endochondral growth components cell volume increase to matrix production was subject to age-dependent and animal-specific changes per cell in the condylar cartilage of the domestic pig. in addition, it was to be investigated whether the cell distribution in the condylar cartilage and thus the tissue differentiation changes as one aspect of the aging process and whether the differentiation differs from that of primary hyali ...	2004	15378192
cloning of host-ptp: the only example of a protein-tyrosine-phosphatase the function of which has been lost between rodent and human.	protein-tyrosine-phosphatases (ptp-ases), in concert with protein tyrosine kinases, control various biological activities such as cell growth and differentiation. in rodents, around 40 ptp-ases have been described. functional orthologue for each of these ptp-ases have been identified in human, except for ost-ptp. ost-ptp is a transmembrane ptp-ase with a restricted tissue distribution. in silico analysis on public sequence databases reveals a human ost-ptp gene orthologue that encompasses 21 kb ...	2004	15358244
an anaesthetic protocol in the young domestic pig allowing neuromuscular blockade for studies of cardiac function following cardioplegic arrest and cardiopulmonary bypass.	neuromuscular blockade should, for ethical reasons, not be allowed in animal experiments unless the use is strongly motivated. beforehand, the anaesthetic protocol must be documented without muscle relaxation in the species studied. documentation is difficult to obtain from the scientific literature. when focusing on cardiac function over time, in particular, the ideal anaesthetic protocol should cause no or minor alterations in cardiac variables.	2004	15352961
laparoscopic warm renal ischemia in the solitary porcine kidney model.	to determine the effect of a laparoscopic approach on warm renal ischemia in the pig with a solitary kidney. although the maximal safe duration for warm ischemia during open partial nephrectomy is commonly accepted to be 30 minutes, the maximal safe ischemic time during laparoscopic partial nephrectomy has not been previously determined.	2004	15351615
bone morphogenetic protein and ceramic-induced osteogenesis.	to investigate the relationship between bone morphogenetic proteins (bmp) and calcium phosphate ceramic-induced osteogenesis in soft tissues, in vitro and in vivo experiments were performed. in an in vitro study, the ability of different calcium phosphate ceramics to absorb bovine bmp (bbmp) from a bbmp solution was tested. in vivo studies included immunohistochemical bmp staining before bone formation in the ceramics was detected, and the enhancement of bone formation in calcium phosphate ceram ...	1998	15348928
laser-assisted end-to-end vascular anastomosis of elastin heterograft to carotid artery with an albumin stent in vivo.	laser-assisted end-to-end vascular anastomosis of an elastin heterograft to native artery may avoid the problems associated with currently available vascular synthetic grafts and conventional suture anastomosis.	2004	15345171
adventitial vasa vasorum heterogeneity among different vascular beds.	different vascular beds show substantial variation in their susceptibilities for development of vascular disease like atherosclerosis, and thereby exhibit a variety of different clinical presentations. yet, the underlying mechanism of this heterogeneity is not well defined. recent evidence suggests a role for the vasa vasorum (vv) in vascular disease. we hypothesized that there is a differential distribution structure of adventitial vv in different vascular beds. hence, the current study was des ...	2004	15337884
phylogenetic relationships of australian and new zealand feral pigs assessed by mitochondrial control region sequence and nuclear gpip genotype.	pigs were introduced into australia and new zealand in the 18th and 19th centuries, with some establishing feral populations. with few records of pig introductions into these two countries, molecular phylogenetic analysis was used to assess their origins. mitochondrial (mt) control region sequence and nuclear glucosephosphate isomerase pseudogene (gpip) restriction fragments were used, as distinct european and asian domestic pig and wild boar control region clades and gpip genotypes can be recog ...	2004	15336669
two african swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities.	african swine fever virus (asfv), a large icosahedral deoxyvirus, is the causative agent of an economically relevant hemorrhagic disease that affects domestic pigs. the major purpose of the present study was to investigate the nuclear transport activities of the asfv p37 and p14 proteins, which result from the proteolytic processing of a common precursor. experiments were performed by using yeast-based nucleocytoplasmic transport assays and by analysis of the subcellular localization of differen ...	2004	15331706
characterisation of trichinella isolates from bulgaria by molecular typing and cross-breeding.	trichinella spp. larvae were collected from domestic and wild-life animals in association with 15 human trichinellosis outbreaks registered between 1999-2002 in bulgaria. furthermore, trichinella spp. isolates were obtained from 62 naturally infected wild animals and of a rat. all isolates were subjected to speciation by both multiplex pcr and cross-breeding experiments. epidemiological and clinical data were collected and analysed using standard protocols for epidemiological surveillance and co ...	2004	15325044
analysis of the successional patterns of insects on carrion in southwest virginia.	studies of carrion-insect succession on domestic pig, sus scrofa l., were conducted in the spring and summer of 2001 and 2002 in blacksburg, va, to identify and analyze the successional patterns of the taxa of forensic importance in southwest virginia. forty-seven insect taxa were collected in the spring. these were represented by 11 families (diptera: calliphoridae, sarcophagidae, muscidae, sepsidae, piophilidae; coleoptera: staphylinidae, silphidae, cleridae, trogidae, dermestidae, histeridae) ...	2004	15311476
scavenger properties of cultivated pig liver endothelial cells.	background: the liver sinusoidal endothelial cells (lsec) and kupffer cells constitute the most powerful scavenger system in the body. various waste macromolecules, continuously released from tissues in large quantities as a consequence of normal catabolic processes are cleared by the lsec. in spite of the fact that pig livers are used in a wide range of experimental settings, the scavenger properties of pig lsec has not been investigated until now. therefore, we studied the endocytosis and intr ...	2004	15306034
paclitaxel balloon coating, a novel method for prevention and therapy of restenosis.	drug-eluting stents have shown promising antirestenotic effects in clinical trials. non-stent-based local delivery of antiproliferative drugs may offer additional flexibility and also reach vessel areas beyond the immediate stent coverage. the aim of the present study was to evaluate a novel method of local drug delivery based on angioplasty balloons.	2004	15302790
effects of vasopressin on left anterior descending coronary artery blood flow during extremely low cardiac output.	because of the possibility of vasopressin-mediated coronary vasospasm, this study was designed to assess effects of vasopressin compared to saline placebo on left anterior descending (lad) coronary artery blood flow. twelve anaesthetized domestic swine were prepared for lad coronary artery blood flow measurement with ultrasonic flow probes, using cardiopulmonary by-pass adjusted to 10% of the prearrest cardiac output. this 10% value approximates that reported for cardiac output during convention ...	2004	15294409
in vivo porcine intradiscal pressure as a function of external loading.	spinal loading during daily activity as it relates to the ability of the intervertebral disc to sustain its integrity has been a major issue in spinal research. the purpose of this investigation was to establish the relationship between the intervertebral disc pressure in the nucleus and the load applied to the motion segment in an in vivo porcine model.	2004	15280761
monoclonal antibody-based immunohistochemical diagnosis of malaysian nipah virus infection in pigs.	formalin-fixed, paraffin wax-embedded tissues of three malaysian farm pigs naturally infected with nipah virus were used to investigate the value of anti-nipah virus mouse monoclonal antibodies (mabs) and rabbit polyclonal antibody for immunohistochemical diagnosis. mabs 11f6 and 12a5 gave intense immunolabelling in lung tissue that had been fixed in 10% neutral buffered formalin for about 4 years, whereas the reactivity of mabs 13a5 and 18c4 and polyclonal antibody was reduced significantly by ...	2004	15276859
the epidemiological investigation of trichinella infection in brown rats (rattus norvegicus) and domestic pigs in croatia suggests that rats are not a reservoir at the farm level.	whether the brown rat (rattus norvegicus) is a reservoir of trichinella spp. infection or merely an accidental host, which may be vector of trichinella spp., continues to be debated. we estimated the prevalence of trichinella sp. infection in brown rat populations and in domestic pigs in 2 villages in croatia, where trichinella sp. infection in pigs has been endemic in the past 10 yr. trichinella spiralis larvae, identified by a multiplex polymerase chain reaction analyses, were the only species ...	2004	15270124
trichinellosis associated with bear meat--new york and tennessee, 2003.	trichinellosis is a parasitic infection caused by tissue-dwelling trichinella roundworms and is associated traditionally with ingestion of pork from infected domestic swine. as a result of improvements in swine production, trichinellosis has declined steadily in the united states. however, infection also can result from eating the meat of wild animals. during 1997-2001, a total of 72 cases of trichinellosis (median: 12 cases annually; range: 11-23 cases) were reported to cdc; the majority of the ...	2004	15254452
phosphorus runoff from incorporated and surface-applied liquid swine manure and phosphorus fertilizer.	excessive fertilization with organic and/or inorganic p amendments to cropland increases the potential risk of p loss to surface waters. the objective of this study was to evaluate the effects of soil test p level, source, and application method of p amendments on p in runoff following soybean [glycine max (l.) merr.]. the treatments consisted of two rates of swine (sus scrofa domestica) liquid manure surface-applied and injected, 54 kg p ha(-1) triple superphosphate (tsp) surface-applied and in ...	2004	15254136
ureteral replacement using small-intestinal submucosa and a collagen inhibitor in a porcine model.	small-intestinal submucosa (sis) has been successful as an onlay graft in ureteral repair, but tubularized segment interposition of sis has been unsuccessful. our objective was to evaluate whether a type i collagen inhibitor, halofuginone, would prevent stricture formation in tubularized sis interposition.	2004	15253833
in vitro culture of epithelial cells from the caput, corpus, and cauda epididymis of sus domesticus.	this work describes a protocol to culture epididymal epithelial cells from the caput, corpus, and cauda regions of sus domesticus. epididymal epithelial fragments were obtained by dissection and enzymatic digestion with collagenase. about 30 epididymal fragments from each epididymal region were cultured in 24-well culture plates with supplemented rpmi-1640 medium at 37 degrees c, 5% co2 in air, and 100% humidity. a confluent monolayer of polygonal and tightly packed epithelioid cells from the th ...	2004	15251244
a slaughter slab survey for extra-intestinal porcine helminth infections in northern tanzania.	a survey on extra-intestinal porcine helminths was conducted at three slaughter slabs that receive pigs from mbulu, a district endemic for porcine cysticercosis in northern tanzania. seventy carcases of pigs between 1 and 2 years old were examined between december 1997 and march 1998. the examination involved ante-mortem lingual examination for taenia solium cysticercosis followed by post-mortem inspection. in addition, a laboratory procedure was performed to determine whether any of these domes ...	2004	15241967
heterosis and epistasis for teat number and fluctuating asymmetry in crosses between jiaxing and iberian pigs.	in the framework of wright's view of evolution, long-separate breeds of domestic animals could establish different adaptive epistatic genetic complexes that could be destroyed in crossbred animals by recombination. the objective of this study was to evaluate heterosis and recombination effects in a crossing experiment involving two distinct european and asian breeds (iberian and jiaxing) in the f1 and two successive backcrosses to the iberian line. teat number (tn) was recorded in the right and ...	2004	15241457
treatment of hypotension in pigs with an inspiratory impedance threshold device: a feasibility study.	an inspiratory impedance threshold device was evaluated in spontaneously breathing animals with hypotension to determine whether it could help improve systemic arterial pressures when fluid replacement was not immediately available.	2004	15241102
closure of the cystic duct during laparoscopic cholecystectomy with a new feedback-controlled bipolar sealing system in case of biliary obstruction--an experimental study in pigs.	the excellent results of new devices like the new bipolar feedback-controlled sealing system (ligasure) for closure of blood vessels encouraged surgeons to use these instruments for other structures like bile ducts. the aim of this study was to evaluate the feasibility of closure of cystic duct in case of biliary obstruction.	2004	15239216
methylprednisolone enhances contraction of porcine femoral head epiphyseal arteries.	the pathomechanism of steroid-induced femoral head necrosis is thought to be disturbed femoral head blood flow. we hypothesize that methylprednisolone increases vasocontraction of femoral head epiphyseal arteries, thereby reducing femoral head blood flow. nine immature female domestic pigs were randomly selected from a group of 18 to receive 24-hour methylprednisolone treatment, whereas the nine remaining pigs received the placebo control in a blinded fashion. after sacrifice, lateral epiphyseal ...	2004	15232435
comparison of systemic responses of radiofrequency ablation, cryotherapy, and surgical resection in a porcine liver model.	the degree of systemic response after hepatic radiofrequency ablation (rfa) has not been well investigated.	2004	15231521
systematic reaction to electrolytic treatment of pig livers in vivo.	only a minority of secondary liver tumours are amenable to segmental resection and as a result, considerable research has been focused on developing ablative methods to destroy liver metastases. many of these methods are limited by the development of a systemic inflammatory response mediated by cytokines such as interleukin-8 (il-8) and tumour necrosis factor alpha (tnf-alpha). the aim of the present study was to determine if a systemic reaction occurred following electrolytic treatment of pig l ...	2004	15230798
an experimental technique for heart-lung transplantation in subprimate models.	the success of heart-lung transplant experiments in subprimate models has been limited because most techniques include total pulmonary denervation, which causes respiratory problems, blood loss, and subsequent death. using 20 subprimates (12 domestic swine and 8 mongrel dogs), we tested a heterotopic heart-single lung transplant method intended to preserve unilateral pulmonary innervation and to reduce blood loss. after native right pneumonectomy, the donor heart and right lung were transplanted ...	1990	15227176
load dependence of cardiac output in biventricular pacing: right ventricular volume overload in pigs.	previous work from our laboratory has demonstrated that optimization of biventricular pacing is load dependent. during acute pulmonary stenosis and right ventricular pressure overload in swine, cardiac output was maximized by pacing the right ventricle 40 ms before the left ventricle. to extend those studies, this experiment examined biventricular pacing optimization during right ventricular volume overload.	2004	15224027
comparative chromosome painting between the domestic pig (sus scrofa) and two species of peccary, the collared peccary (tayassu tajacu) and the white-lipped peccary (t. pecari): a phylogenetic perspective.	the suidae and the dicotylidae (or tayassuidae) are related mammalian families, both belonging to the artiodactyl suborder suiformes, which diverged more than 37 million years ago. cross-species chromosome painting was performed between the domestic pig (sus scrofa; 2n = 38), a representative of the suidae, and two species of the dicotylidae: the collared peccary (tayassu tajacu; 2n = 30) and the white-lipped peccary (t. pecari; 2n = 26). g-banded metaphase chromosomes of the two peccaries were ...	2004	15218266
retrograde coronary venous perfusion at low pressure.	the effectiveness of localized retrograde coronary venous perfusion (rcvp) in preventing or reversing myocardial ischemia after acute ligation of a coronary artery is described. ten domestic pigs (group i) underwent aorto left anterior coronary vein grafting with rcvp at systemic pressure. in another set of ten pigs (group ii), the coronary vein was similarly grafted, but the proximal end of the graft was perfused with oxygenated blood by means of an external pump at reduced pressure and flow. i ...	1980	15216280
african swine fever virus proteins involved in evading host defence systems.	african swine fever virus (asfv) can cause an acutely fatal haemorrhagic fever in domestic pigs although in its natural hosts, warthogs, bushpigs and the soft tick vector, ornithodoros moubata, asfv causes inapparent persistent infections. the virus is a large, cytoplasmic, double-stranded dna virus which has a tropism for macrophages. as it is the only member of the asfarviridae family, asfv encodes many novel genes not encoded by other virus families. the ability of the virus to persist in its ...	2004	15207450
comparison of methods of temperature measurement in swine.	the purpose of these experiments was to test the equivalence of pulmonary artery, urinary bladder, tympanic, rectal and femoral artery methods of temperature measurement in healthy and critically ill swine under clinical intensive care unit (icu) conditions using a prospective, time series design. first, sensors were tested for error and sensitivity to change in temperature with a precision-controlled water bath and a laboratory-certified digital thermometer for temperatures 34-42 degrees c. the ...	2004	15207041
an isoenzyme survey of trypanosoma brucei s.l. from the central african subregion: population structure, taxonomic and epidemiological considerations.	in order to improve our knowledge about the taxonomic status and the population structure of the causative agent of human african trypanosomiasis in the central african subregion, 169 newly isolated stocks, of which 16 came from pigs, and 5 reference stocks, were characterized by multilocus enzyme electrophoresis, for 17 genetic loci. we identified 22 different isoenzyme profiles or zymodemes, many of which showed limited differences between them. these zymodemes were equated to multilocus genot ...	2004	15206467
detection and genetic characterization of porcine circovirus type 2 (pcv2) in pigs from croatia.	porcine circovirus type 2 (pcv2) from the circoviridae family has recently been associated with two serious diseases of swine, post-weaning multisystemic wasting syndrome (pmws) and porcine dermatitis and nephropathy syndrome (pdns). during 2002, several outbreaks of clinical disease in pigs with weights ranging from 10 to 70 kg occurred on four farms in different locations in croatia. the signs were consistent with pmws and pdns. apart from progressive weight loss, pneumonia and/or diarrhoea, m ...	2004	15196907
in vitro coronary artery imaging with sixteen-slice helical computed tomography.	to determine the benefits of submillimeter coronary computed tomography (ct) angiography in vitro.	2004	15191099
initial study of arthropod succession on pig carrion in a central european urban habitat.	we conducted a carrion succession study within a restricted urban backyard in the city of vienna, austria (16 degrees 22'e, 48 degrees 12'n) from may to november 2001 to analyze sequence and composition of the local carrion visiting fauna. two medium sized clothed domestic pig carcasses (sus scrofa linnaeus), were used as surrogate human models. in total, 42 arthropod species from the families calliphoridae, sarcophagidae, sepsidae, piophilidae, muscidae, fanniidae, sphaeroceridae, phoridae, dro ...	2004	15185958
load dependence of cardiac output in biventricular pacing: right ventricular pressure overload in pigs.	the effect of biventricular pacing on stroke volume is believed to be dependent on right ventricular/left ventricular delay, but effects in individual patients are unpredictable. this variability may reflect relative right and left ventricular volume and/or pressure overloads. accordingly, we tested the hypothesis that the relation of cardiac output to right ventricular/left ventricular delay is load dependent in a pig model of pulmonary stenosis.	2004	15173728
ultrasound strain imaging of altered myocardial stiffness: stunned versus infarcted reperfused myocardium.	in this study we evaluate the diastolic deformation of ischemic/reperfused myocardium and relate this deformation to tissue elastic properties.	2004	15173032
splanchnic metabolism during gut ischemia and short-term endotoxin and hemorrhagic shock as evaluated by intravasal microdialysis.	the splanchnic area is of considerable interest in different types of shock. to characterize the metabolic changes in the splanchnic region in response to different types of shock we used a model where shock-induced metabolic changes in the splanchnic region were studied by the use of intravasal microdialysis. 23 anesthetized domestic pigs were randomized into four groups: group i, serving as controls (n = 5); group ii, mesenteric ischemia for 180 followed by 120 min of reperfusion (n = 5); grou ...	2004	15167688
interaction between a perfluorocarbon emulsion and radiographic contrast media.	this study evaluated specially designed perfluorocarbon (pfc) emulsions as blood substitutes in case of induced ischemia of the left heart ventricle in healthy farm pigs. two hundred ml of perfluorocarbon emulsion were infused while 200 ml of blood were simultaneously drawn. radiographic contrast media were given to aid placement of balloon catheters in the left coronary artery. histopathological analysis showed that right heart failure caused the deaths of both pigs. particles (up to>3 micro) o ...	2004	15152157
a novel population of calretinin-positive neurons comprises reelin-positive cajal-retzius cells in the hippocampal formation of the adult domestic pig.	calretinin-containing neurons in the hippocampal formation, including the subiculum, presubiculum, parasubiculum, and entorhinal cortex, were visualized with immunocytochemistry. calretinin immunoreactivity was present exclusively in non-principal cells. the largest immunoreactive cell population was found in the outer half of the molecular layer of the dentate gyrus and in the stratum lacunosum-moleculare of ammon's horn. a proportion of these cells were also immunoreactive for reelin, a cajal- ...	2004	15132437
delayed portal vein thrombosis after experimental radiofrequency ablation near the main portal vein.	portal venous blood flow may protect adjacent tumour cells from thermal destruction with radiofrequency ablation (rfa). this study aimed to investigate the local effect of rfa on the main portal vein branch, and the completeness of cellular ablation in its vicinity, with or without a pringle manoeuvre using a porcine model.	2004	15122617
the effects of birth weight and postnatal growth patterns on fat depth and plasma leptin concentrations in juvenile and adult pigs.	low birth weight is associated with altered adipose tissue deposition and regulation of leptin production. this study determined the effects of naturally occurring variations in birth weight in pigs on postnatal growth patterns, body fat depth and plasma leptin and other hormone concentrations. low (< 1.47 kg) and high (> 1.53 kg) birth weight piglets were studied at 3 months (juvenile; n= 47) and 12 months of age (young adult; n= 17). at each age, arterial and venous catheters were inserted und ...	2004	15121800
emerging encephalitogenic viruses: lyssaviruses and henipaviruses transmitted by frugivorous bats.	three newly recognized encephalitogenic zoonotic viruses spread from fruit bats of the genus pteropus (order chiroptera, suborder megachiroptera) have been recognised over the past decade. these are: hendra virus, formerly named equine morbillivirus, which was responsible for an outbreak of disease in horses and humans in brisbane, australia, in 1994; australian bat lyssavirus, the cause of a severe acute encephalitis, in 1996; and nipah virus, the cause of a major outbreak of encephalitis and p ...	2004	15119765
acute myocardial infarction: contrast-enhanced multi-detector row ct in a porcine model.	to assess the role of contrast material-enhanced retrospectively electrocardiographically (ecg) gated multi-detector row computed tomography (ct) in the detection of acute myocardial infarction in a porcine model of total coronary occlusion.	2004	15118118
surgical construction of an in vivo carotid siphon model to test neurovascular devices.	we developed an animal model to evaluate vascular trauma induced by endovascular devices that are proposed for the treatment of cerebrovascular diseases.	2004	15113480
immunogenicity and t cell recognition in swine of foot-and-mouth disease virus polymerase 3d.	immunization of domestic pigs with a vaccinia virus (vv) recombinant expressing foot-and-mouth disease virus (fmdv) 3d protein conferred partial protection against challenge with infectious virus. the severity reduction of the clinical symptoms developed by the challenged animals occurred in the absence of significant levels of anti-3d circulating antibodies. this observation suggested that the partial protection observed was mediated by the induction of a 3d-specific cellular immune response. t ...	2004	15110524
epidemiological study on porcine circovirus type 2 (pcv2) infection in the european wild boar (sus scrofa).	porcine circovirus type 2 (pcv2) is considered as the causative agent of postweaning multisystemic wasting syndrome (pmws) in domestic pigs, where the virus is ubiquitous as evidenced by serological surveys. we present the results of the first nationwide sero-survey on the presence of pcv2 antibodies in european wild boars, and report the first pmws case in a wild boar from spain. sera from 656 hunter harvested wild boars from 45 different geographical sites and 22 additional imported animals we ...	2004	15099500
new metallic mr stents for artifact-free coronary mr angiography: feasibility study in a swine model.	the objective of this study was to investigate the potential for artifact-free coronary magnetic resonance angiography (cmra) in the presence of dedicated metallic mr stents in vitro and in a swine model.	2004	15087718
linkage disequilibrium in the domesticated pig.	this study investigated the extent of linkage disequilibrium (ld) in two genomic regions (on chromosomes 4 and 7) in five populations of domesticated pigs. ld was measured with d' and tested for significance with the fisher exact test. effects of genetic (linkage) distance, chromosome, population, and their interactions on d' were tested both through a linear model analysis of covariance and by a theoretical nonlinear model. the overall result was that (1) the distance explained most of the vari ...	2004	15082558
prevalence of antibodies to hepatitis e virus among apparently healthy humans and pigs in bali, indonesia: identification of a pig infected with a genotype 4 hepatitis e virus.	in indonesia where hepatitis e virus (hev) is believed to be highly endemic, only three outbreaks of hev transmission have been documented to date in restricted areas (west kalimantan and east java). a total of 1,115 serum samples collected from apparently healthy individuals in bali, lombok, and surabaya in indonesia in 1996 where epidemic hev transmissions have never been reported, were tested for igg class antibodies to hev (anti-hev). in bali, anti-hev was detected in 20% (54/276) of the tes ...	2004	15042646
novel herpesviruses of suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses.	five novel herpesviruses were identified in suid species from africa (common warthog, phacochoerus africanus) and south-east asia (bearded pig, sus barbatus; babirusa, babyrousa babyrussa) by detection and analysis of their dna polymerase genes. three of the novel species, p. africanus cytomegalovirus 1, p. africanus lymphotropic herpesvirus 1 (pafrlhv-1) and s. barbatus lymphotropic herpesvirus 1 (sbarlhv-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [ ...	2004	15039528
[histological investigation of the micromorphological effects of the application of a laser needle--results of an animal experiment].	in an experimental animal study (sus scrofa domesticus) we investigated the effects of the new technique of laser needle stimulation (wavelength: 685 nm; energy density: 4.6 kj/cm2 per point; application duration: 20 min). the results revealed changes in microcirculatory parameters of the skin resulting in an increase in blood flow. however, the quality and intensity of the laser light did not induce micromorphological alterations in the skin.	2004	15032490
prevalence of porcine endogenous retrovirus in domestic pigs in japan and its potential infection in dogs xenotransplanted with porcine pancreatic islet cells.	the prevalence of porcine endogenous retrovirus (perv) proviral dna among various pig breeds raised in japan was investigated by polymerase chain reaction (pcr). moreover, potential infection of perv was investigated by pcr and reverse transcriptase-polymerase chain reaction (rt-pcr) in experimentally induced diabetic dogs (n=5) implanted with the diffusion chamber type bio-artificial endocrine pancreas (bio-aep) containing porcine pancreatic endocrine (pe) cells. no immunosuppressant was used a ...	2004	15031539