Publications

consolidating tactical planning and implementation frameworks for integrated vector management in uganda.	integrated vector management (ivm) is the recommended approach for controlling some vector-borne diseases (vbd). in the face of current challenges to disease vector control, ivm is vital to achieve national targets set for vbd control. though global efforts, especially for combating malaria, now focus on elimination and eradication, ivm remains useful for uganda which is principally still in the control phase of the malaria continuum. this paper outlines the processes undertaken to consolidate t ...	2016	27074809
detection of all four dengue serotypes in aedes aegypti female mosquitoes collected in a rural area in colombia.	the aedes aegypti vector for dengue virus (denv) has been reported in urban and periurban areas. the information about denv circulation in mosquitoes in colombian rural areas is limited, so we aimed to evaluate the presence of denv in ae. aegypti females caught in rural locations of two colombian municipalities, anapoima and la mesa. mosquitoes from 497 rural households in 44 different rural settlements were collected. pools of about 20 ae. aegypti females were processed for denv serotype detect ...	0	27074252
identification and genomic analysis of a novel group c orthobunyavirus isolated from a mosquito captured near iquitos, peru.	group c orthobunyaviruses are single-stranded rna viruses found in both south and north america. until very recently, and despite their status as important vector-borne human pathogens, no group c whole genome sequences containing all three segments were available in public databases. here we report a group c orthobunyavirus, named el huayo virus, isolated from a pool of culex portesi mosquitoes captured near iquitos, peru. although initial metagenomic analysis yielded only a handful of reads be ...	2016	27074162
assessing potential countermeasures against the dengue epidemic in non-tropical urban cities.	dengue is a common mosquito-borne viral disease epidemic especially in tropical and sub-tropical regions where water sanitation is not substantially controlled. however, dengue epidemics sometimes occur in non-tropical urban cities with substantial water sanitary control. using a mathematical model, we investigate what conditions can be important for a dengue epidemic to occur in an urban city such as tokyo, where vectors are active only in summer and there are little number of vectors around ho ...	2016	27072122
computational approach towards exploring potential anti-chikungunya activity of selected flavonoids.	chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ...	2016	27071308
zika virus outbreak in rio de janeiro, brazil: clinical characterization, epidemiological and virological aspects.	in 2015, brazil was faced with the cocirculation of three arboviruses of major public health importance. the emergence of zika virus (zikv) presents new challenges to both clinicians and public health authorities. overlapping clinical features between diseases caused by zikv, dengue (denv) and chikungunya (chikv) and the lack of validated serological assays for zikv make accurate diagnosis difficult.	2016	27070912
zika virus in a traveler returning to china from caracas, venezuela, february 2016.		2016	27070735
estimating risks of importation and local transmission of zika virus infection.	background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ...	2016	27069825
epidemiological dynamics of an urban dengue 4 outbreak in são paulo, brazil.	dengue studies at the urban scale are scarce and required for guiding control efforts. in brazil, the burden of dengue is high and challenges city public health administrations with limited resources. here we studied the dynamics of a dengue epidemic in a single city.	2016	27069820
spectrum of maternofetal outcomes during dengue infection in pregnancy: an insight.	dengue is a vector transmitted viral infection; tropical and subtropical countries see outbreaks of dengue each year. there is a paucity of literature on effects of dengue infection on pregnancy outcome and this prompted us to undertake a study for better understanding of pregnancy implications with dengue infection. pregnant women admitted during the seasonal outbreak of dengue between september 2015 and october 2015 were studied and maternal and fetal outcomes in sixteen ns1ag positive women w ...	2016	27069349
vector surveillance for dengue virus detection in the archipelago of fernando de noronha, brazil.	aedes aegypti (l.) has become an efficient vector of important arboviruses due to its anthropophilic and domiciliary behaviors. since the 1980s, dengue affects thousands of people every year in brazil; in fernando de noronha (fn), a touristic archipelago, dengue cases have occurred since 2001. once ae. aegypti populations are well established in the inhabited areas of fn, the threat of dengue or another arbovirus epidemic is continuously imminent. this study aimed to monitor the denv serotypes i ...	2016	27067800
on the seasonal occurrence and abundance of the zika virus vector mosquito aedes aegypti in the contiguous united states.	an ongoing zika virus pandemic in latin america and the caribbean has raised concerns that travel-related introduction of zika virus could initiate local transmission in the united states (u.s.) by its primary vector, the mosquito aedes aegypti.	2016	27066299
how to predict molecular interactions between species?	organisms constantly interact with other species through physical contact which leads to changes on the molecular level, for example the transcriptome. these changes can be monitored for all genes, with the help of high-throughput experiments such as rna-seq or microarrays. the adaptation of the gene expression to environmental changes within cells is mediated through complex gene regulatory networks. often, our knowledge of these networks is incomplete. network inference predicts gene regulator ...	2016	27065992
homology modeling and molecular docking studies of drosophila and aedes sex peptide receptors.	the drosophila melanogaster sex peptide receptor (drmspr), which is a g protein-coupled receptor (gpcr), is known as the specific receptor for sex peptide (sp). it is responsible for the reproductive behavior in the drosophila model system; in particular, it is involved in the post-mating responses such as the increase in egg-laying ability and decrease in receptivity in females. in a previous study, we discovered a small molecule agonist of drmspr for the first time, which could not, however, a ...	2016	27060892
functional validation of the carbon dioxide receptor in labial palps of helicoverpa armigera moths.	adult moths possess an organ in their labial palps, the labial-palp pit organ, which is specialized for sensing carbon dioxide (co2). they use co2 as a cue to detect healthy plants and find food or lay eggs on them. the molecular bases of the co2 receptor in drosophila melanogaster and aedes aegypti have been reported, but the molecular mechanisms of the co2 receptor in lepidoptera remains elusive. in this study, we first re-examined three putative helicoverpa armigera co2 gustatory receptor gen ...	2016	27060445
molecular cloning, sequence analysis and developmental stage expression of a putative septin gene fragment from aedes albopictus (diptera: culicidae).	septins belong to gtpases that are involved in vital cellular activities, including cytokinesis. although present in many organisms, they are yet to be isolated from aedes albopictus. this study reports for the first time on a serendipitous isolation of a partial septin sequence from ae. albopictus and its developmental expression profile. the ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. it shares homology with several insect septin genes and has a cl ...	2016	27059016
harnessing the power of defensive microbes: evolutionary implications in nature and disease control.		2016	27058881
neglected tropical diseases in the anthropocene: the cases of zika, ebola, and other infections.		2016	27058728
indole alkaloids of the stigonematales (cyanophyta): chemical diversity, biosynthesis and biological activity.	the cyanobacteria are well recognized as producers of a wide array of bioactive metabolites including toxins, and potential drug candidates. however, a limited number of taxa are generally considered with respect to both of these aspects. that said, the order stigonematales, although largely overlooked in this regard, has become increasingly recognized as a source of bioactive metabolites relevant to both human and environmental health. in particular, the hapalindoles and related indole alkaloid ...	2016	27058546
in silico screening, alanine mutation, and dft approaches for identification of ns2b/ns3 protease inhibitors.	to identify the ligand that binds to a target protein with high affinity is a nontrivial task in computer-assisted approaches. antiviral drugs have been identified for ns2b/ns3 protease enzyme on the mechanism to cleave the viral protein using the computational tools. the consequence of the molecular docking, free energy calculations, and simulation protocols explores the better ligand. it provides in-depth structural insights with the catalytic triad of his51, asp75, ser135, and gly133. the md ...	2016	27057355
projection of climate change influences on u.s. west nile virus vectors.	while estimates of the impact of climate change on health are necessary for health care planners and climate change policy makers, models to produce quantitative estimates remain scarce. we describe a freely available dynamic simulation model parameterized for three west nile virus vectors, which provides an effective tool for studying vector-borne disease risk due to climate change. the dynamic mosquito simulation model is parameterized with species specific temperature-dependent development an ...	2015	27057131
quantitative analysis of harmonic convergence in mosquito auditory interactions.	this article analyses the hearing and behaviour of mosquitoes in the context of inter-individual acoustic interactions. the acoustic interactions of tethered live pairs of aedes aegypti mosquitoes, from same and opposite sex mosquitoes of the species, are recorded on independent and unique audio channels, together with the response of tethered individual mosquitoes to playbacks of pre-recorded flight tones of lone or paired individuals. a time-dependent representation of each mosquito's non-stat ...	0	27053654
mosquito vectors and the spread of cancer: an overlooked connection?	mosquitoes (diptera: culicidae) represent a key threat for millions of humans and animals worldwide, vectoring important pathogens and parasites, including malaria, dengue, filariasis, and zika virus. besides mosquito-borne diseases, cancers figure among the leading causes of mortality worldwide. it is expected that annual cancer cases will rise from 14 million in 2012 to 22 million within the next two decades. notably, there are few contrasting evidences of the relationship between cancer and m ...	2016	27053131
why zika virus infection has become a public health concern?	prior to 2015, zika virus (zikv) outbreaks had occurred in areas of africa, southeast asia, and the pacific islands. although a causal relationship between zika infection during pregnancy and microcephaly is strongly suspected, such a connection has not yet been scientifically proven. in may 2015, the outbreak of zikv infection in brazil led to reports of syndrome and pregnant women giving birth to babies with birth defects and poor pregnancy outcomes; the pan american health organization (paho) ...	2016	27052792
biological and chemical characterization of a new larvicide ovitrap made of plastic with pyriproxyfen incorporated for aedes aegypti (diptera: culicidae) control.	aedes aegypti (l.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. ovitraps are containers constructed to imitate aedes' natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. in addition to their value for vector surveillance, various ovit ...	2016	27052297
response of culex quinquefasciatus larvae to three insecticides.		0	27049713
genetic drift during systemic arbovirus infection of mosquito vectors leads to decreased relative fitness during host switching.	the emergence of mosquito-borne rna viruses, such as west nile virus (wnv), is facilitated by genetically complex virus populations within hosts. here, we determine whether wnv enzootic (culex tarsalis, cx. quinquefasciatus, and cx. pipiens) and bridge vectors (aedes aegypti) have differential impacts on viral mutational diversity and fitness. during systemic mosquito infection, wnv faced stochastic reductions in genetic diversity that rapidly was recovered during intra-tissue population expansi ...	2016	27049584
zika virus: implications for public health.	the world health organization has declared the current zika virus (zikv) epidemic a public health emergency of international concern. lack of vaccines and reliable diagnostic tests, broad geographical distribution of mosquito species that can transmit the virus, and absence of population immunity in newly affected countries are causes for concern. although most infected persons are asymptomatic, zikv has been associated with a rise in cases of neurological complications and fetal central nervous ...	2016	27048745
comparative pharmacological characterization of d1-like dopamine receptors from anopheles gambiae, aedes aegypti and culex quinquefasciatus suggests pleiotropic signaling in mosquito vector lineages.	small molecule antagonists of mosquito dopamine receptors (dars) are under investigation as a new class of vector-selective insecticides. antagonists that inhibit the d1-like dars aadop2 and cqdop2 from the mosquitoes aedes aegypti l. and culex quinquefasciatus say, respectively, also cause larval mortality in bioassays. here, we report on the orthologous dar, agdop2, from the malaria mosquito anopheles gambiae giles that was cloned and pharmacologically characterized in hek293 cells. larval bio ...	2016	27048546
larvicidal, biological and genotoxic effects, and temperature-toxicity relationship of some leaf extracts of nerium oleander (apocynaceae) on culex pipiens (diptera: culicidae).	the present study was undertaken to study the larvicidal activity of different extracts of nerium oleander leaves, and post-treatment temperature- toxicity relationship of these extracts against culex pipiens. further, the most potent extract was used to evaluate its biological and genotoxic activities.	2015	27047967
risk associated with the release of wolbachia-infected aedes aegypti mosquitoes into the environment in an effort to control dengue.	in an effort to eliminate dengue, a successful technology was developed with the stable introduction of the obligate intracellular bacteria wolbachia pipientis into the mosquito aedes aegypti to reduce its ability to transmit dengue fever due to life shortening and inhibition of viral replication effects. an analysis of risk was required before considering release of the modified mosquito into the environment.	2016	27047911
fungal biological control agents for integrated management of culicoides spp. (diptera: ceratopogonidae) of livestock.	entomopathogenic fungi metarhizium anisopliae and beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. thus, research was aimed to explore the potency of these fungal spores against larval and adult culicoides midges, a pest of livestock.	2015	27047065
identification of aadnr1, a novel gene related to innate immunity and apoptosis in aedes albopictus.	innate immunity and apoptosis play critical roles in defending pathogens in insects. in drosophila, dnr1 was reported as a negative regulator of apoptosis and immune deficiency (imd) pathway which belongs to innate immunity. aedes albopictus is an important kind of arbovirus vector and becoming a significant threat to public health due to its rapid global expansion. here we identified an ortholog of dnr1 from a. albopictus, named as aadnr1. aadnr1 encoded a putative protein containing an n-termi ...	2016	27045774
evaluating the effectiveness of localized control strategies to curtail chikungunya.	chikungunya, a re-emerging arbovirus transmitted to humans by aedes aegypti and ae. albopictus mosquitoes, causes debilitating disease characterized by an acute febrile phase and chronic joint pain. chikungunya has recently spread to the island of st. martin and subsequently throughout the americas. the disease is now affecting 42 countries and territories throughout the americas. while chikungunya is mainly a tropical disease, the recent introduction and subsequent spread of ae. albopictus into ...	2016	27045523
vaccination of gerbils with bm-103 and bm-ral-2 concurrently or as a fusion protein confers consistent and improved protection against brugia malayi infection.	the brugia malayi bm-103 and bm-ral-2 proteins are orthologous to onchocerca volvulus ov-103 and ov-ral-2, and which were selected as the best candidates for the development of an o. volvulus vaccine. the b. malayi gerbil model was used to confirm the efficacy of these ov vaccine candidates on adult worms and to determine whether their combination is more efficacious.	2016	27045170
presence of three dengue serotypes in ouagadougou (burkina faso): research and public health implications.	the significant malaria burden in africa has often eclipsed other febrile illnesses. burkina faso's first dengue epidemic occurred in 1925 and the most recent in 2013. yet there is still very little known about dengue prevalence, its vector proliferation, and its poverty and equity impacts.	2016	27044528
efficacy of four commercially available heartworm preventive products against the jyd-34 laboratory strain of dirofilaria immitis.	heartworm disease in dogs can be severe and life threatening. resistance to available heartworm preventives was considered among potential causes of increased reports of failed heartworm prevention in dogs. the objective of the present study was to compare the efficacy of four commercially available heartworm disease preventives against the jyd-34 strain of d. immitis.	2016	27044379
tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål).	the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ...	2016	27043527
can radiology play a role in early diagnosis of dengue fever?	dengue fever (df) is a viral hemorrhagic fever causing severe morbidity and mortality in affected patients.	0	27042608
tiger mosquito - generated devastation in the biosphere!!!		2016	27041891
trypsin inhibitor from leucaena leucocephala seeds delays and disrupts the development of aedes aegypti, a multiple-disease vector.	nowadays, the aedes aegypti mosquito represents a serious public health issue in view of the large outbreaks of the arboviral diseases zika, dengue, chikungunya and yellow fever. this holometabolous insect has midgut digestive enzymes that are trypsin- and chymotrypsin-like proteins. protease inhibitors are able to bind to proteolytic enzymes and promote a blockage in digestion and nutrition, leading to death. thus, we investigated the effect of trypsin inhibitor of leucaena leucocephala (lti) s ...	2017	27040615
identification of abcc2 as a binding protein of cry1ac on brush border membrane vesicles from helicoverpa armigera by an improved pull-down assay.	cry1ac toxin-binding proteins from helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling cry1ac to cnbr agarose combined with liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to the lc-ms/ms results, cry1ac toxin could bind to six classes of aminopeptidase-n, alkaline phosphatase, cadherin-like protein, atp-binding cassette transporter subfamily c protein (abcc2), actin, atpase, polycalin, and some other pro ...	2016	27037552
mosquito host choices on livestock amplifiers of rift valley fever virus in kenya.	animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. however, these parameters remain poorly understood for mosquito vectors of the rift valley fever (rvf), an arboviral disease, and for a community of mosquitoes.	2016	27036889
knowledge, attitude, and practices regarding vector-borne diseases in western jamaica.	outbreaks of vector-borne diseases (vbds) such as dengue and malaria can overwhelm health systems in resource-poor countries. environmental management strategies that reduce or eliminate vector breeding sites combined with improved personal prevention strategies can help to significantly reduce transmission of these infections.	0	27036722
recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine.	dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ...	2016	27035715
prediction of dengue outbreaks based on disease surveillance and meteorological data.	research is needed to create early warnings of dengue outbreaks to inform stakeholders and control the disease. this analysis composes of a comparative set of prediction models including only meteorological variables; only lag variables of disease surveillance; as well as combinations of meteorological and lag disease surveillance variables. generalized linear regression models were used to fit relationships between the predictor variables and the dengue surveillance data as outcome variable on ...	2016	27031524
a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer.	dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ...	2016	27030492
zika virus.	zika virus (zikv) is an arthropod-borne virus (arbovirus) in the genus flavivirus and the family flaviviridae. zikv was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in africa, and zikv infections in humans were sporadic for half a century before emerging in the pacific and the americas. zikv is usually transmitted by the bite of infected mosquitoes. the clinical presentation of zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especiall ...	2016	27029595
insect transformation with piggybac: getting the number of injections just right.	the insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggybac is the transposable element most commonly utilized for insect germline transformation. the described efficiency of this process is variable in the published literature, and a comprehensive review of transformation efficiency in insects is lacking. this study compared and contrasted ...	2016	27027400
dengue virus-1 infection did not alter the behavioral response of aedes aegypti (diptera: culicidae) to deet.	no licensed vaccine or antiviral drug against dengue virus (denv) is available; therefore, most of the effort to prevent this disease is focused on reducing vector-host interactions. one of the most widely accepted methods of blocking vector-human contact is to use insect repellents to interfere with mosquito host-seeking behavior. some arboviruses can replicate in the nervous system of the vector, raising the concern that arboviral infection may alter the insect behavioral response toward chemi ...	2016	27026163
rnai trigger delivery into anopheles gambiae pupae.	rna interference (rnai), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. the ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. in the major human malaria vector anopheles gambiae, the predominant me ...	2016	27023367
characterization of a novel murine model to study zika virus.	the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ...	2016	27022155
long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children.	we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ...	2016	27022153
socioeconomic and ecological factors influencing aedes aegypti prevalence, abundance, and distribution in dhaka, bangladesh.	this study examined household risk factors and prevalence, abundance, and distribution of immature aedes aegypti and aedes albopictus, and their association with socioeconomic and ecological factors at urban zonal and household levels in the city of dhaka, bangladesh. during the 2011 monsoon, 826 households in 12 randomly selected administrative wards were surveyed for vector mosquitoes. results revealed that the abundance and distribution of immature ae. aegypti and ae. albopictus, and pupae-pe ...	2016	27022149
first report of leptolegnia chapmanii (peronosporomycetes: saprolegniales) affecting mosquitoes in central brazil.	numerous isolates of an oomycete 'fungus', leptolegnia chapmanii, are reported from brazil for the first time. this aquatic pathogen was baited with aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central brazilian city of goiânia and from more distant sites in the western and northern regions of the state of goiás. isolates were identified based on their morphological and developmental characters, compa ...	2016	27018147
viruses causing hemorrhagic fever. safety laboratory procedures.	viral hemorrhagic fevers are diseases caused by viruses which belong to different families, many of them causing severe diseases. these viruses may produce different symptomatology together with a severe multisystem syndrome, and the final result might be the production of hemorrhages in several sites of the body. the majority of them have no other treatment than supportive therapy, although some antiviral drugs can be used in some circumstances. transmission of vhf has been demonstrated through ...	2016	27014378
ambivalent outcomes of cell apoptosis: a barrier or blessing in malaria progression.	the life cycle of plasmodium in two evolutionary distant hosts, mosquito, and human, is a complex process. it is regulated at various stages of developments by a number of diverged mechanisms that ultimately determine the outcome of the disease. during the development processes, plasmodium invades a variety of cells in two hosts. the invaded cells tend to undergo apoptosis and are subsequently removed from the system. this process also eliminates numerous parasites along with these apoptotic cel ...	2016	27014225
genetic variations in bionomics of culex quinquefasciatus (diptera: culicidae) mosquito population in minna, north central nigeria.	the need to have an improved knowledge on the bioecology of culex quinquefasciatus, a prerequisite in the development of cost-effective control strategies, has informed the present preliminary investigation to put in better perspective variations that exist in the egg rafts of the species. freshly laid egg rafts were collected and incubated at ambient temperature in well-labeled plastic trays. the results showed overall inconsistency in all indices monitored for the egg rafts. generally, survivo ...	2016	27013900
the emergence of zika virus as a global health security threat: a review and a consensus statement of the indusem joint working group (jwg).	the zika virus (zikv), first discovered in 1947, has emerged as a global public health threat over the last decade, with the accelerated geographic spread of the virus noted during the last 5 years. the world health organization (who) predicts that millions of cases of zikv are likely to occur in the americas during the next 12 months. these projections, in conjunction with suspected zika-associated increase in newborn microcephaly cases, prompted who to declare public health emergency of intern ...	0	27013839
identification and characterization of two "sensory neuron membrane proteins" (snmps) of the desert locust, schistocerca gregaria (orthoptera: acrididae).	pheromone-responsive neurons of insects not only require specific receptors but in addition several auxiliary components, including the "sensory neuron membrane protein," snmp. accordingly, snmp is considered as a marker for neurons responding to pheromones. for the desert locust schistocerca gregaria, it is known that the behavior, including aggregation behavior and courtship inhibition, is largely controlled by pheromones. however, little is known about pheromones, their receptors, and the phe ...	2016	27012870
virtual screening of plant volatile compounds reveals a high affinity of hylamorpha elegans (coleoptera: scarabaeidae) odorant-binding proteins for sesquiterpenes from its native host.	hylamorpha elegans(burmeister) is a native chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. the study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (obps), offers interesting opportunities to identify new compounds ...	2016	27012867
dengue virus: a global human threat: review of literature.	dengue is an acute viral illness caused by rna virus of the family flaviviridae and spread by aedes mosquitoes. presenting features may range from asymptomatic fever to dreaded complications such as hemorrhagic fever and shock. a cute-onset high fever, muscle and joint pain, myalgia, cutaneous rash, hemorrhagic episodes, and circulatory shock are the commonly seen symptoms. oral manifestations are rare in dengue infection; however, some cases may have oral features as the only presenting manifes ...	0	27011925
fitting the incidence data from the city of campinas, brazil, based on dengue transmission modellings considering time-dependent entomological parameters.	four time-dependent dengue transmission models are considered in order to fit the incidence data from the city of campinas, brazil, recorded from october 1st 1995 to september 30th 2012. the entomological parameters are allowed to depend on temperature and precipitation, while the carrying capacity and the hatching of eggs depend only on precipitation. the whole period of incidence of dengue is split into four periods, due to the fact that the model is formulated considering the circulation of o ...	2016	27010654
the retinoid x receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.	in arthropods, retinoid x receptor (rxr) is a highly conserved nuclear hormone receptor. by forming a heterodimeric complex with the ecdysone receptor (ecr), rxr is known to be vital importance for various physiological processes. however, in comparison to ecr, the rxr signaling pathway and its roles in crustacean reproduction are poorly understood. in the present study, the rxr mrna was detected in the ovarian follicular cells of mud crab scylla paramamosain (sprxr) and during ovarian maturatio ...	2016	27009370
primary dengue virus infections induce differential cytokine production in mexican patients.	severe dengue pathogenesis is not fully understood, but high levels of proinflammatory cytokines have been associated with dengue disease severity. in this study, the cytokine levels in 171 sera from mexican patients with primary dengue fever (df) and dengue haemorrhagic fever (dhf) from dengue virus (denv) 1 (n = 116) or 2 (n = 55) were compared. df and dhf were defined according to the patient's clinical condition, the primary infections as indicated by igg enzymatic immunoassay negative resul ...	0	27008374
correction: how diverse detrital environments influence nutrient stoichiometry between males and females of the co-occurring container mosquitoes aedes albopictus, ae. aegypti, and culex quinquefasciatus.		2016	27007775
introduction and transmission of zika virus in brazil: new challenges for the americas.		2016	27007567
maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage.	epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ...	2016	27007501
digestion of yeasts and beta-1,3-glucanases in mosquito larvae: physiological and biochemical considerations.	aedes aegypti larvae ingest several kinds of microorganisms. in spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. we investigated the effects of using yeasts as the sole nutrient source for a. aegypti larvae. we also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. larvae were fed with cat food (control ...	2016	27007411
identification of protease m1 zinc metalloprotease conferring resistance to deltamethrin by characterization of an aflp marker in culex pipiens pallens.	continuous and excessive application of deltamethrin (dm) has resulted in the rapid development of insecticide resistance in culex pipiens pallens. the quantitative trait loci (qtl) responsible for resistance to dm had previously been detected in cx. pipiens pallens. but locating the qtls on the chromosomes remained difficult. an available approach is to first characterize dna molecular markers linked with the phenotype, and then identify candidate genes.	2016	27007119
the chikungunya virus: an emerging us pathogen.	the chikungunya (chik) virus was recently reported by the cdc to have spread to the united states. we report an early documented case of chik from the state of pennsylvania after a patient recently returned from haiti in june of 2014.	0	27006742
zika virus: yet another emerging threat to nepal.	zika virus (zikv) is a flavivirus with single stranded rna related to yellow fever, dengue, west nile, and japanese encephalitis viruses and is transmitted by aedes mosquitoes primarily by aedes aegipti which is widely distributed in nepal. zikv was first identified incidentally in rhesus monkey in uganda in 1947 and human infection in 1952; and by now outbreaks of zikv disease have been recorded in africa, the americas, asia and the pacific. the world health organization (who) has recently decl ...	2016	27005721
larvicidal and pupicidal activities of alizarin isolated from roots of rubia cordifolia against culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae).	the mosquitocidal activities of different fractions and a compound alizarin from the methanol extract of rubia cordifolia roots were evaluated on larvae and pupae of culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae). larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10 ppm for fractions and 0.5, 1.0, 1.5 and 2.0 ppm for compound. after 24 h, the mortality was assessed and the lc50 and lc90 values were estimated for larvae and pupae. among the 23 fractions ...	2016	27004695
the response of claudin-like transmembrane septate junction proteins to altered environmental ion levels in the larval mosquito aedes aegypti.	septate junctions (sjs) occlude the paracellular pathway and function as paracellular diffusion barriers within invertebrate epithelia. however, integral components of sjs and their contribution to barrier properties have received considerably less attention than those of vertebrate occluding junctions. in arthropods, sj proteins have only been identified in drosophila and among these are three integral claudin-like proteins, megatrachea (mega), sinuous (sinu) and kune-kune (kune), as well as a ...	2016	27004691
ecdysis period and rate deviations of dengue mosquito vector, aedes aegypti reared in different artificial water-holding containers.	artificial water-holding containers (awhcs) have been well-documented in many aedes aegypti studies for dengue surveillance and developmental research. hence, we investigated the role of different ahwcs on the development and ecdysis period of ae. aegypti dengue vector, a container breeding mosquito.	2016	27004577
mosquito adulticidal activity of the leaf extracts of spondias mombin l. against aedes aegypti l. and isolation of active principles.	aedes aegypti is a domestic mosquito and one of the primary vectors for dengue and yellow fever. since, it is a vector of deadly diseases, its control becomes essential. medicinal plants may be an alternative to adulticidal agents since they contain rich source of bioactive compounds. this study was designed to determine the adulticidal activity of spondias mombin leaf methanol crude extract, n-hexane, dichloromethane and ethyl acetate fractions against female adults of ae. aegypti mosquitoes an ...	2016	27004574
genome-wide analysis of ionotropic receptors provides insight into their evolution in heliconius butterflies.	in a world of chemical cues, smell and taste are essential senses for survival. here we focused on heliconius, a diverse group of butterflies that exhibit variation in pre- and post-zygotic isolation and chemically-mediated behaviors across their phylogeny. our study examined the ionotropic receptors, a recently discovered class of receptors that are some of the most ancient chemical receptors.	2016	27004525
plasmodium delichoni n. sp.: description, molecular characterisation and remarks on the exoerythrocytic merogony, persistence, vectors and transmission.	malaria parasite plasmodium (novyella) delichoni n. sp. (haemosporida, plasmodiidae) was found in a widespread eurasian songbird, the common house martin delichon urbicum (hirundinidae). it is described based on the morphology of its blood stages and segments of the mitochondrial cytochrome b and apicoplast genes, which can be used for molecular identification of this species. erythrocytic meronts and gametocytes are strictly nucleophilic, and mature gametocytes possess pigment granules of marke ...	2016	27000087
a deep insight into the sialome of male and female aedes aegypti mosquitoes.	only adult female mosquitoes feed on blood, while both genders take sugar meals. accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. we performed de novo assembly of reads from adult aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). by mapp ...	2016	26999592
outbreak of zika virus disease in the americas and the association with microcephaly, congenital malformations and guillain-barré syndrome.		2016	26998633
changes in the physicochemical properties of piperine/β-cyclodextrin due to the formation of inclusion complexes.	piperine (pp) is a pungent component in black pepper that possesses useful biological activities; however it is practically insoluble in water. the aim of the current study was to prepare a coground mixture (gm) of pp and β-cyclodextrin (βcd) (molar ratio of pp/βcd = 1/1) and subsequently evaluate the solubility of pp and physicochemical properties of the gm. dsc thermal behavior of the gm showed the absence of melting peak of piperine. pxrd profile of the gm exhibited halo pattern and no charac ...	2016	26998357
incremental cost of implementing residual insecticide treatment with delthametrine on top of intensive routine aedes aegypti control.	information on the cost of implementing residual insecticide treatment (rit) for aedes control is scarce. we evaluated the incremental cost on top of intensive conventional routine activities of the aedes control programme (acp) in the city of santiago de cuba, cuba.	2016	26996279
minocycline as a re-purposed anti-wolbachia macrofilaricide: superiority compared with doxycycline regimens in a murine infection model of human lymphatic filariasis.	lymphatic filariasis and onchocerciasis are parasitic helminth diseases, which cause severe morbidities such as elephantiasis, skin disease and blindness, presenting a major public health burden in endemic communities. the anti-wolbachia consortium (a·wol: http://www.a-wol.com/) has identified a number of registered antibiotics that target the endosymbiotic bacterium, wolbachia, delivering macrofilaricidal activity. here we use pharmacokinetics/pharmacodynamics (pk/pd) analysis to rationally dev ...	2016	26996237
chemical composition, toxicity and non-target effects of pinus kesiya essential oil: an eco-friendly and novel larvicide against malaria, dengue and lymphatic filariasis mosquito vectors.	mosquitoes (diptera: culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide, with special reference to tropical and subtropical countries. the overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. therefore, the development of eco-friendly control tools is an important public health challenge. in this study, the mosquito larvicidal activity of pinus ke ...	2016	26995063
wolbachia modulates lipid metabolism in aedes albopictus mosquito cells.	certain strains of the intracellular endosymbiont wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (denv) by aedes mosquitoes, and the mechanisms responsible are still not well understood. direct infusion and liquid chromatography-fourier transform ion cyclotron resonance (ft-icr) mass spectrometry-based lipidomics analyses were conducted using aedes albopictus aa23 cells that were infected with the wmel and wmelpop strains of wolbachia in comparison to un ...	2016	26994075
composition and biological activities of the essential oil of piper corcovadensis (miq.) c. dc (piperaceae).	essential oil from fresh leaves of the shrub piper corcovadensis (miq.) c. dc was obtained in 0.21% (w/w) yield by hydrodistillation in a clevenger type apparatus. thirty-one components, accounting for 96.61% of the leaf oil, were identified by gas chromatography-mass spectrometry. the major constituents of the oil were 1-butyl-3,4-methylenedioxybenzene (30.62%), terpinolene (17.44%), trans -caryophyllene (6.27%), α-pinene (5.92%), δ-cadinene (4.92%), and limonene (4.46%). bioassays against larv ...	2016	26993082
the zika pandemic - a perfect storm?		2016	26991663
dna barcoding and wing morphometrics to distinguish three aedes vectors in thailand.	aedes aegypti (diptera: culicidae) (l.), ae. albopictus (skuse), and ae. scutellaris (walker) are important mosquito vectors of dengue and chikungunya viruses. they are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. any confusion could have a negative impact on epidemiological studies or control strategies. therefore, we explored two modern tools to supplement current morphological identification: d ...	2016	26987285
is dengue vector control deficient in effectiveness or evidence?: systematic review and meta-analysis.	although a vaccine could be available as early as 2016, vector control remains the primary approach used to prevent dengue, the most common and widespread arbovirus of humans worldwide. we reviewed the evidence for effectiveness of vector control methods in reducing its transmission.	2016	26986468
revision to cdc's zika travel notices: minimal likelihood for mosquito-borne zika virus transmission at elevations above 2,000 meters.	since may 2015, when zika virus, a flavivirus transmitted primarily by aedes aegypti mosquitoes, was reported in brazil, the virus has rapidly spread across the region of the americas and the caribbean. the association between maternal zika virus infection and adverse fetal and reproductive outcomes, including microcephaly, prompted cdc to issue a level 2 alert travel notice* for the 37 countries and u.s. territories (at the national and territorial level) that have reported recent zika virus tr ...	2016	26985965
horizontal transfer of β-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes.	horizontal gene transfer (hgt) is a movement of genetic information occurring outside of normal mating activities. it is especially common between prokaryotic endosymbionts and their protozoan, insect, and nematode hosts. although beta carbonic anhydrase (β-ca) plays a crucial role in metabolic functions of many living organisms, the origin of β-ca genes in eukaryotic species remains unclear.	2016	26983858
finding aedes aegypti in a natural breeding site in an urban zone, sao paulo, southeastern brazil.	this is the description of how nine aedes aegypti larvae were found in a natural breeding site in the pinheiros neighborhood, city of sao paulo, sp, southeastern brazil. the record was conducted in december 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. findingae. aegypti larvae in a tree hole shows this species' ability to use both artificial and natural environments as breeding sites a ...	2016	26982959
comparison of the epidemiological aspects of imported dengue cases between korea and japan, 2006-2010.	to compare the epidemiological characteristics of dengue cases imported by travelers or immigration in both korea and japan, we determined dengue incidence and related risk factors. during 2006-2010, 367 and 589 imported dengue cases were reported in korea and japan, respectively. in korea, the presumptive origins for the dengue infections were southeast asia (82.6%), southern asia (13.9%), eastern asia (1.1%), south america (0.3%), central america (0.3%), africa (0.3%), and other countries (1.6 ...	2015	26981346
a disease around the corner.		2016	26981335
longevity of animals under reactive oxygen species stress and disease susceptibility due to global warming.	the world is projected to experience an approximate doubling of atmospheric co2 concentration in the next decades. rise in atmospheric co2 level as one of the most important reasons is expected to contribute to raise the mean global temperature 1.4 °c-5.8 °c by that time. a survey from 128 countries speculates that global warming is primarily due to increase in atmospheric co2 level that is produced mainly by anthropogenic activities. exposure of animals to high environmental temperatures is mos ...	0	26981200
comparative genomic analysis of pre-epidemic and epidemic zika virus strains for virological factors potentially associated with the rapidly expanding epidemic.	less than 20 sporadic cases of human zika virus (zikv) infection were reported in africa and asia before 2007, but large outbreaks involving up to 73% of the populations on the pacific islands have started since 2007, and spread to the americas in 2014. moreover, the clinical manifestation of zikv infection has apparently changed, as evident by increasing reports of neurological complications, such as guillain-barré syndrome in adults and congenital anomalies in neonates. we comprehensively comp ...	2016	26980239
silencing of the olfactory co-receptor gene in dendroctonus armandi leads to eag response declining to major host volatiles.	in this study, a polymerase chain reaction (pcr) based on homology genes of orco was utilized to identify darmorco, which is essential for olfaction in d. armandi. the results showed that darmorco shares significant sequence homology with orco proteins had known in other insects. quantitative real-time pcr (qrt-pcr) analysis suggested that darmorco was abundantly expressed in adult d. armandi; by contrast, darmorco showed trace amounts of expression level in other stages. of different tissues, d ...	2016	26979566
identification of a tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.	the saliva of tsetse flies contains a cocktail of bioactive molecules inducing specific antibody responses in hosts exposed to bites. we have previously shown that an indirect-elisa test using whole salivary extracts from glossina morsitans submorsitans was able to discriminate between (i) cattle from tsetse infested and tsetse free areas and (ii) animals experimentally exposed to low or high numbers of tsetse flies. in the present study, our aim was to identify specific salivary synthetic pepti ...	2016	26979518
sfdredd, a novel initiator caspase possessing activity on effector caspase substrates in spodoptera frugiperda.	sf9, a cell line derived from spodoptera frugiperda, is an ideal model organism for studying insect apoptosis. the first notable study that attempted to identify the apoptotic pathway in sf9 was performed in 1997 and included the discovery of sf-caspase-1, an effector caspase of sf9. however, it was not until 2013 that the first initiator caspase in sf9, sfdronc, was discovered, and the apoptotic pathway in sf9 became clearer. in this study, we report another caspase of sf9, sfdredd. sfdredd is ...	2016	26977926
evolutionary analysis of dengue serotype 2 viruses using phylogenetic and bayesian methods from new delhi, india.	dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. delhi, the metropolitan capital state of india, has reported many dengue outbreaks, with the last outbreak occurring in 2013. we have recently reported predominance of dengue virus serotype 2 during 2011-2014 in delhi. in the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in delhi. envelope genes ...	2016	26977703