Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [effect of heat on the different types of perfringens toxin]. | 1951 | 14896564 | |
| the role of clostridium welchii in strangulation obstruction. | 1951 | 14895126 | |
| examination of strains of clostridium welchii isolated from teeth. | 1951 | 14886445 | |
| the isolation of clostridium welchii from human teeth. | 1951 | 14886444 | |
| alkaline phosphatase activity of the kidney following experimental shock in dogs induced by clostridium perfringens toxins. | 1952 | 14884836 | |
| [characterization of the various types of cl. perfringens: remarks on anti-gangrene serums]. | 1951 | 14865372 | |
| [five cases of perfringens septicemia]. | 1951 | 14840704 | |
| [research on bacterial collagenase]. | 1951 | 14840665 | |
| the acra test as a means of estimating hyaluronidase, deoxyribonuclease and their antibodies. | 1951 | 14832689 | |
| foetal and placental gas gangrene infection in a case of eclampsia. | 1951 | 14832672 | |
| [extraction of toxin from welchia agni var. wilsdoni (perfringens d) from bacterial bodies]. | 1951 | 14829939 | |
| intraocular infection with the gas-forming bacillus clostridium welchii; report of a case. | 1951 | 14829112 | |
| [experimental comparative study of the histamine formation of b. enterotoxicus and other pathogenous anaerobic bacteria]. | 1951 | 14825744 | |
| [effect of para-aminobenzoic acid and certain derivatives on clostridium perfringens and on the hemotoxin in culture]. | 1950 | 14815346 | |
| [effect of sulfonamide preparations on clostridium perfringens type a and on their production of hemotoxins]. | 1950 | 14815345 | |
| [differential diagnosis of clostridium perfringens based on certain biochemic group properties]. | 1950 | 14815344 | |
| case report: gas gangrene; report of a case with unusual response to treatment. | 1951 | 14814705 | |
| the action of collagenase on skin and the anti-collagenase factor in human serum. | 1951 | 14803822 | |
| acute pneumocholecystitis, with report of a case. | 1950 | 14801822 | |
| inhibition of succinoxidase activity of mitochondria by clostridium welchii toxin. | 1950 | 14800904 | |
| effect of experimental shock induced by clostridium perfringens toxin on the kidneys of dogs. | 1951 | 14799033 | |
| [demonstration of b. enterotoxicus in tissue]. | 1950 | 14798941 | |
| [experimental enteritis in the dog caused by peroral infection with b. enterotoxicus]. | 1950 | 14798940 | |
| cl. welchii infections as problems in bacterial intoxication. | 1950 | 14797700 | |
| gas bacillus infection of abdomen following appendectomy. | 1950 | 14795154 | |
| single cell culture of cl. welchii type a morphologically changed by penicillin. | 1950 | 14791907 | |
| the action of penicillin on cl. welchii type a. | 1950 | 14791906 | |
| turbidimetric measurement of natural antitoxin to clostridium welchii alpha-toxin. | 1950 | 14784908 | |
| [panophthalmitis due to clostridium welchi]. | 1950 | 14784011 | |
| the gram complex in clostridium welchii. | 1950 | 14780186 | |
| [effect of sulfonamides para-aminobenzoic acid, procaine and para-aminosalicylic acid on hemotoxin production by clostridium perfringens type a]. | 1949 | 14779874 | |
| [analysis of the effect of penicillin on hemotoxin production by clostridium perfringens type a]. | 1949 | 14779873 | |
| enzymes of clostridium welchii type a and clostridium histolyticum that disintegrate decalcified human tooth dentine. | 1950 | 14778940 | |
| the migration of cultures of clostridium perfringens in semisolid medium. | 1950 | 14774346 | |
| the turbidity-producing action of clostridium perfringens toxin in human sera. | 1950 | 14771630 | |
| development and validation of experimental protocols for use of cardinal models for prediction of microorganism growth in food products. | an experimental protocol to validate secondary-model application to foods was suggested. escherichia coli, listeria monocytogenes, bacillus cereus, clostridium perfringens, and salmonella were observed in various food categories, such as meat, dairy, egg, or seafood products. the secondary model validated in this study was based on the gamma concept, in which the environmental factors temperature, ph, and water activity (aw) were introduced as individual terms with microbe-dependent parameters, ... | 2004 | 14766591 |
| the role of bacterial and non-bacterial toxins in the induction of changes in membrane transport: implications for diarrhea. | bacterial toxins induce changes in membrane transport which underlie the loss of electrolyte homeostasis associated with diarrhea. bacterial- and their secreted toxin-types which have been linked with diarrhea include: (a) vibrio cholerae (cholera toxin, e1 tor hemolysin and accessory cholera enterotoxin); (b) escherichia coli (heat stable enterotoxin, heat-labile enterotoxin and colicins); (c) shigella dysenteriae (shiga-toxin); (d) clostridium perfringens (c. perfringens enterotoxin, alpha-tox ... | 2003 | 14757199 |
| use of reflectors to enhance the synergistic effects of solar heating and solar wavelengths to disinfect drinking water sources. | aluminum reflectors were added to solar units designed to inactivate faecal microorganisms (faecal coliform, e. coli, enterococci, frna coliphage, c. perfringens) in stream water and diluted sewage by the two mechanisms (solar heat, solar uv) known to inactivate microorganisms. during sunny conditions, solar units with and without reflectors inactivated e. coli to <1 cfu/100 ml to meet drinking water standards. solar units with reflectors disinfected the water sooner by increasing the water temp ... | 2003 | 14753571 |
| [an autopsy case of the hepatocellular carcinoma associated with multiple myeloma which developed fatal massive hemolysis due to the clostridium perfringens septicemia following tae]. | 2003 | 14748326 | |
| crystallization and preliminary x-ray analysis of the human-specific toxin intermedilysin. | intermedilysin is a human-specific toxin from streptococcus intermedius, which is part of normal human oral flora. the bacterium is an opportunistic pathogen with a tendency for deep-seated infection in the brain and liver. intermedilysin belongs to the cholesterol-dependent cytolysin (cdcs) family of toxins, which have been identified in several different bacteria including the serious human pathogens s. pneumoniae and clostridium perfringens. intermedilysin, however, is the only member that sh ... | 2004 | 14747721 |
| [generalized pain syndrome, fever and somnolence in an 81-year-old patient]. | we present a case of an 81-year-old diabetic man with anaerobic sepsis due to acalculous cholecystitis. the patient was admitted to our hospital with a seven-day history of severe abdominal pain accompanied with fever and somnolence. blood cultures taken during the initial procedure developed clostridium perfringens. the patient was immediately treated with parenteral penicillin. the ultrasonography pointed out the case: the gall bladder was found to be distended and slightly thickened. this res ... | 2004 | 14735245 |
| enteritis necroticans with recurrent enterocutaneous fistulae caused by clostridium perfringens in a child with cyclic neutropenia. | 2004 | 14734887 | |
| swine waste treatment by self-heating aerobic thermophilic bioreactors. | pig manure represents a very high-strength wastewater that is well suited for a self-heating aerobic thermophilic treatment. here we report the use of 59-l aerobic thermophilic sequencing batch reactors (at-sbr) to study the treatment of pig manure with a hrt of 6 days. temperatures up to 75 degrees c were reached without external heating by using venturi-type aerators but these conditions were detrimental for the respiratory activity of the microflora. for cod removal, better performances were ... | 2004 | 14723922 |
| clostridium perfringens pleuropulmonary infection and septic shock: case report and population-based laboratory surveillance study. | we report a rare case of necrotizing pneumonia, empyema, and septic shock caused by clostridium perfringens. population-based laboratory surveillance was subsequently conducted that identified an annual incidence of invasive c. perfringens disease of 0.83 per 100,000 population with a striking age-related increased risk for acquisition. | 2003 | 14723369 |
| antibacterial efficiency of finnish spice essential oils against pathogenic and spoilage bacteria. | the antibacterial properties of 13 essential oils, derived from spices grown in finland, were examined with an agar diffusion method against 12 bacterial strains. the organisms tested included both spoilage and pathogenic bacteria. the gram-positive bacteria appeared to be more sensitive than the gram-negative organisms, clostridium botulinum and clostridium perfringens being the most sensitive. oregano, savory, and thyme showed the broadest antibacterial activity by distinctly inhibiting the gr ... | 2004 | 14717375 |
| growth of heat-treated enterotoxin-positive clostridium perfringens and the implications for safe cooling rates. | clostridium perfringens 790-94 and 44071.c05 carrying a chromosomal and a plasmid cpe gene, respectively, were used to determine differences in heat resistance and growth characteristics between the genotypes. heat inactivation experiments were conducted using an immersed coil apparatus. spore germination, outgrowth, and lag phase, together named gol time, as well as generation times were determined during constant temperatures in fluid thioglycollate (ftg) medium as well as in vacuum-packed, he ... | 2004 | 14717356 |
| primary gas gangrene of the pancreas: report of a case. | spontaneous gas gangrene of the pancreas, caused by an infection of clostridium perfringens, is an extremely rare but severe form of acute pancreatitis. a 67-year-old man complaining of severe epigastric pain with diffuse guarding underwent an emergency laparotomy. during surgery, hemorrhagic pancreatic necrosis was observed with a large amount of peripancreatic gas. cultures demonstrated c. perfringens. the identification of hemolysis and the accumulation of peripancreatic gas on computed tomog ... | 2004 | 14714236 |
| campylobacter spp., giardia spp., cryptosporidium spp., noroviruses, and indicator organisms in surface water in southwestern finland, 2000-2001. | a total of 139 surface water samples from seven lakes and 15 rivers in southwestern finland were analyzed during five consecutive seasons from autumn 2000 to autumn 2001 for the presence of various enteropathogens (campylobacter spp., giardia spp., cryptosporidium spp., and noroviruses) and fecal indicators (thermotolerant coliforms, escherichia coli, clostridium perfringens, and f-rna bacteriophages) and for physicochemical parameters (turbidity and temperature); this was the first such systema ... | 2004 | 14711629 |
| the clostridium perfringens teta(p) efflux protein contains a functional variant of the motif a region found in major facilitator superfamily transport proteins. | the clostridium perfringens tetracycline resistance protein, teta(p), is an inner-membrane protein that mediates the active efflux of tetracycline from the bacterial cell. this protein comprises 420 aa and is predicted to have 12 transmembrane domains (tmds). comparison of the teta(p) amino acid sequence to that of several members of the major facilitator superfamily (mfs) identified a variant copy of the conserved motif a. this region consists of the sequence e59xpxxxxxdxxxrk72 and is located w ... | 2004 | 14702405 |
| clostridium perfringens alpha-toxin activates the sphingomyelin metabolism system in sheep erythrocytes. | clostridium perfringens alpha-toxin induces hemolysis of rabbit erythrocytes through the activation of glycerophospholipid metabolism. sheep erythrocytes contain large amounts of sphingomyelin (sm) but not phosphatidylcholine. we investigated the relationship between the toxin-induced hemolysis and sm metabolic system in sheep erythrocytes. alpha-toxin simultaneously induced hemolysis and a reduction in the levels of sm and formation of ceramide and sphingosine 1-phosphate (s1p). n-oleoylethanol ... | 2004 | 14702348 |
| synthesis and evaluation as sialidase inhibitors of xylo-configured cyclohexenephosphonates carrying glycerol side-chain mimics. | based on a strategy previously reported by us, we have synthesized d-xylo configured cyclohexenephosphonates designed to mimic the transition state of the sialidase reaction. the double bond orientation corresponds to the benchmark inhibitor neu5ac2en and we could selectively introduce hydroxyalkyl substituents in order to simulate the glycerol side-chain of neuraminic acid. the inhibitory activity of a set of compounds towards bacterial sialidases was tested and interesting differences in activ ... | 2004 | 14698159 |
| the development of tolerance to clostridium perfringens type d epsilon-toxin in mdck and g-402 cells. | the epithelial madin darby canine kidney (mdck) cell line, caucasian renal leiomyoblastoma (g-402) cells, human small airways epithelial (hsae) cells, human bronchial epithelial (hbe) cells and human renal proximal tubule (hrpt) epithelial cells were examined for sensitivity to clostridium perfringens biotype d epsilon-toxin. mdck and g-402 cells were confirmed as being the only established cell lines that are sensitive to the toxin. hsae, hbe and hrpt epithelial cells were only found to be sens ... | 2003 | 14686482 |
| maternal vaccination against subclinical necrotic enteritis in broilers. | the inclusion of antibacterial feed additives has until now been the major strategy for controlling clostridium perfringens-associated necrotic enteritis in broilers. in the present study, the effect of maternal immunization against the disease was examined. broiler breeder hens were injected intramuscularly with candidate vaccines based on c. perfringens type a and type c toxoids adjuvanted with aluminium hydroxide. vaccination resulted in a strong serum immunoglobulin g response to c. perfring ... | 2004 | 14681072 |
| fatal necrotic enteritis associated with clostridium perfringens in wild crows (corvus macrorhynchos). | sporadic outbreaks of fatal enteritis occurred among free-living wild crows ('large billed' or 'wok' crow; corvus macrorhynchos) in an open-air park in japan in 2002. eight crows were found dead during february, followed by two more in september, and five of the eight were examined histopathologically. at necropsy, all cases showed a markedly dilated small intestine, especially the jejunum and ileum, with large amounts of gas, and dark red to greenish-brown soft content. the necrotic intestinal ... | 2004 | 14681064 |
| susceptibility of clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. | clostridium perfringens strains isolated in 2002 from the intestines of broiler chickens from 31 different farms located in belgium were tested for susceptibility to 12 antibiotics used for therapy, growth promotion or prevention of coccidiosis. all strains were uniformly sensitive to the ionophore antibiotics monensin, lasalocid, salinomycin, maduramycin and narasin. all were sensitive to avilamycin, tylosin and amoxicillin, while flavomycin (bambermycin) showed low or no activity. chlortetracy ... | 2004 | 14681061 |
| microbiology of charcoal-broiled european river lampreys (lampetra fluviatilis) stored at 3 and 22 degrees c. | the microbiological quality of 30 production lots of charcoal-broiled river lampreys was studied at three lamprey processing plants (plants a, b, and c). samples were taken directly after charcoal broiling and stored at 22 and 3 degrees c. lampreys were examined on the day of manufacture, and those kept at 22 degrees c were examined every second day for 6 days. samples kept at 3 degrees c were examined every fourth day for up to 24 days. on the production day, the mean aerobic plate counts (apcs ... | 2003 | 14672233 |
| necrotic hepatitis due to clostridium perfringens infection in newly hatched broiler chicks. | multiple necrotic hepatitis lesions of 5 newly hatched broiler chicks in three flocks derived from two hatcheries were examined pathologically. the livers were brittle, and multiple yellowish or green foci were scattered on the surface and cut surface. the main histological finding was well demarcated multi-focal necrosis in the liver. many gram-positive large bacilli that reacted positively with polyclonal anti-clostridium perfringens serum were observed in necrotic areas. | 2003 | 14665757 |
| clostridium perfringens type a myonecrosis in a horse in korea. | acute hemorrhagic myonecrosis accompanied by severe inter- and intrafascicular edema and hemorrhage of the right gluteal area was diagnosed in a 13-year-old male thoroughbred horse. once the muscular and fascicular changes were subsided, the horse then developed acute respiratory problem. histologically, the lung had diffuse severe hemorrhage with mild neutrophilic infiltration. the cause of death was acute respiratory failure that is believed to occur secondary to toxaemic event. alpha and beta ... | 2003 | 14665756 |
| [characterization of a cl. perfringens type d strain, isolated in the field and optimization of epsilon toxin biosynthesis in a cell culture]. | a field strain of cl. perfringens, named dt001, was isolated from kidney of ovine enterotoemia case. the isolate characterized as cl. perfringens, type d was based on its cultural and biochemical characters and its factors of virulence. the strain was very toxinogenic and well adapted to culture conditions of biofermentation when the parameters related to ptt, incubation time, substrat ... were optimized. thus, the use of carbon source as polymer (destrine), the continuous control of ph allowed ... | 2000 | 14658231 |
| clostridial endophthalmitis after cornea transplantation--florida, 2003. | endophthalmitis is a severe condition caused by inflammation of the ocular cavity that often is associated with infection of the internal structures of the eye. the source of infection can include bacteria disseminated through the bloodstream and contamination of the cornea at the time of ocular surgery or trauma. complications include rapid, reversible vision loss that can progress quickly to panophthalmitis, requiring surgical removal of the eye. clostridium perfringens, an anaerobic gram-posi ... | 2003 | 14654765 |
| evaluation of in vitro properties of di-tri-octahedral smectite on clostridial toxins and growth. | clostridial colitis and endotoxaemia of intestinal origin are significant causes of morbidity and mortality in horses. intestinal adsorbents are available for treatment of these conditions; however, little information exists supporting their use. | 2003 | 14649353 |
| distribution of sewage pollution around a maritime antarctic research station indicated by faecal coliforms, clostridium perfringens and faecal sterol markers. | this study describes the distribution of sewage pollution markers (faecal coliforms, clostridium perfringens and faecal sterols) in seawater and marine sediments around rothera research station, antarctic peninsula. untreated sewage waste has been released from this site since 1975, creating the potential for long-term contamination of the benthic environment. faecal coliform concentrations in seawater reached background levels within 300 m of the outfall. in sediment cores, both c. perfringens ... | 2004 | 14638291 |
| gamma-secretase activity is present in rafts but is not cholesterol-dependent. | cholesterol has been claimed to be involved in the generation and/or accumulation of amyloid beta protein (abeta). however, the underlying molecular mechanisms have not been fully elucidated yet. here, we have investigated the effect of membrane cholesterol content on gamma-secretase activity using chinese hamster ovary cells stably expressing beta-amyloid precursor protein (app) and either wild-type or n141i mutant-type presenilin 2. cholesterol was acutely depleted from the isolated membrane b ... | 2003 | 14636066 |
| dietary effects on bifidobacteria and clostridium perfringens in the canine intestinal tract. | dietary effects on the intestinal microflora have gained increasing interest because of the evidence that a balanced micro ecology in the gut is important for health and well being. the aim of the present study was to evaluate the effect of different diets on faecal counts of bifidobacteria and clostridium perfringens in dogs. two extruded, dry diets, one supplemented with 3% chicory (1.5% inulin), a non-digestible oligosaccharide (ndo) and the other with 3% glucose (glu) were compared with a pr ... | 2003 | 14633049 |
| jerusalem artichokes stimulate growth of broiler chickens and protect them against endotoxins and potential cecal pathogens. | control of intestinal pathogens during the earliest phases of broiler production may be the best strategy for the reduction of human pathogens on processed broiler carcasses. the recent ban on antibiotics in poultry feed has served to focus much attention on alternative methods of controlling the gastrointestinal microflora. a field trial was conducted to evaluate the effect of the fructan-rich jerusalem artichoke, or topinambur (administered as 0.5% topinambur syrup in drinking water), on cultu ... | 2003 | 14627303 |
| polish experience with sewage sludge dewatering in reed systems. | macrophyte plants e.g. reed, cattails, bulrush, can be applied to sewage sludge utilisation. one of the first facilities of this type in the gdansk region (northern poland) was established in darzlubie in 1995 and primary sludge has been utilised in reed beds there. the objective of the undertaken research was evaluating the influence of sewage sludge storage on its chemical and biological properties. a total of 5.5 m thick layer of primary, anaerobically stabilised sludge (moisture 90-96%) was ... | 2003 | 14621154 |
| necrotizing enterotyphlocolitis in dogs treated with a potent antimuscarinic. | to assess the safety of a potent muscarinic receptor antagonist (antimuscarinic) for human clinical testing, repeat-dose oral toxicity studies were conducted in the beagle dog. treatment resulted in unexpected gastrointestinal system effects that were likely mediated by the exaggerated pharmacologic effects of the antimuscarinic on intestinal motility. dogs developed profound anorexia followed by severe bloody diarrhea, and at necropsy, the intestinal tract was distended, filled with red-tinged ... | 2003 | 14608028 |
| duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ... | 2003 | 14605150 |
| a new bacteroides conjugative transposon that carries an ermb gene. | the erythromycin resistance gene ermb has been found in a variety of gram-positive bacteria. this gene has also been found in bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. one of the six bacteroides ermb-containing isolates, wh207, could transfer ermb to bacteroides thetaiotaomicron strain bt4001 by conjugation. wh207 was identified as a bacteroides uniformis strain based on the sequence of its 16s rrna gene. results ... | 2003 | 14602600 |
| a novel type of dna curvature present in a clostridium perfringens ferredoxin gene: characterization and role in gene expression. | this study has revealed that a clostridium perfringens ferredoxin gene (per-fdx) possesses a novel type of dna curvature, which is formed by five phased a-tracts extending from upstream to downstream of the -35 region. the three a-tracts upstream of the promoter and the two within the promoter are located at the positions corresponding to a-tracts present in a c. perfringens phospholipase c gene (plc) and a clostridium pasteurianum ferredoxin gene (pas-fdx), respectively. dna fragments of the pe ... | 2003 | 14600220 |
| molecular structure and evolution of the conjugative multiresistance plasmid pre25 of enterococcus faecalis isolated from a raw-fermented sausage. | plasmid pre25 from enterococcus faecalis transfers resistances against kanamycin, neomycin, streptomycin, clindamycin, lincomycin, azithromycin, clarithromycin, erythromycin, roxithromycin, tylosin, chloramphenicol, and nourseothricin sulfate by conjugation in vitro to e. faecalis jh2-2, lactococcus lactis bu2, and listeria innocua l19. its nucleotide sequence of 50237 base pairs represents the largest, fully sequenced conjugative multiresistance plasmid of enterococci (plasmid 46 (2001) 170). t ... | 2003 | 14597005 |
| usefulness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to clostridium perfringens. | clostridium perfringens is ubiquitous in nature and normally detectable in human stools. therefore, it is difficult to perform specific microbiologic diagnosis in foodborne outbreaks, particularly when only a few cultures are detected from fecal specimens. usually, it has been necessary to detect over 10(6) spores/g of fecal sample as a diagnostic criterion of diarrhea due to c. perfringens. a relatively large foodborne outbreak occurred in osaka city, japan in october 2001. although c. perfring ... | 2003 | 14596965 |
| antimicrobial activity of essential oil and methanol extracts of achillea sintenisii hub. mor. (asteraceae). | the essential oil, obtained by clevenger distillation, and water-soluble and water-insoluble parts of the methanol extracts of achillea sintenisii hub. mor. were individually assayed for their antimicrobial activities against 12 bacteria and two yeasts, candida albicans and c. krusei. no activity was exhibited by the water-soluble subfraction, whereas both the water-insoluble subfraction of the methanol extracts and the essential oil were found to be active against some test microorganisms studi ... | 2003 | 14595577 |
| wessex shopping basket survey - a structured approach to local food sampling. | the shopping basket approach to food sampling, in which ready-to-eat food items from a shopping list were sampled at premises chosen at random, was adopted by 15 environmental health departments in the wessex region. a total of 2037 samples were analysed over a 30-month period. the microbiological quality varied considerably between food categories, with gravy and stock samples giving the highest proportion of satisfactory results. sliced meats, cooked rice and sandwiches gave the poorest overal ... | 2003 | 14594701 |
| biliary tract infection in patients with acute biliary pancreatitis. | the presence of infective microorganisms in the bilio-pancreatic tract is believed to be important in both the onset and outcome of acute biliary pancreatitis. in this study, the characteristics of bile colonization or infection in human pancreatitis were investigated in order to optimize prophylactic antibiotic therapy. | 2003 | 14588158 |
| consumer food-handling behaviors associated with prevention of 13 foodborne illnesses. | to be effective in reducing the incidence of foodborne illness, consumers and food safety educators need information about behaviors that will decrease exposure to foodborne pathogens. a four-round delphi technique was used to survey nationally recognized experts in food microbiology, epidemiology, food safety education, and food safety policy with the aim of identifying and ranking food-handling and consumption behaviors associated with 13 major foodborne pathogens. the food safety experts rank ... | 2003 | 14572229 |
| bactericidal effects of konjac fluid on several food-poisoning bacteria. | in this study, the bactericidal effects of japanese alkaline foods on food-poisoning bacteria were evaluated. konjac is an alkaline food soaked in calcinated calcium (the ph of konjac fluid ranges from 11.42 to 12.53). konjac fluids completely inactivated escherichia coli, enterohemorrhagic e. coli o157:h7 and e. coil o26:h9, salmonella enteritidis, vibrio parahemolyticus. and staphylococcus aureus. the initial level of 6 log cfu/ml dramatically decreased after incubation with konjac fluid, and ... | 2003 | 14572219 |
| clostridium absonum alpha-toxin: new insights into clostridial phospholipase c substrate binding and specificity. | clostridium absonum phospholipase c (caa) is a 42.7 kda protein, which shows 60% amino acid sequence identity with the clostridium perfringens phospholipase c, or alpha-toxin (cpa), and has been isolated from patients suffering from gas gangrene. we report the cloning and sequencing, purification, characterisation and crystal structure of the caa enzyme. caa had twice the phospholipid-hydrolysing (lecithinase) activity, 1.5 times the haemolytic activity and over seven times the activity towards ... | 2003 | 14568535 |
| disinfection efficiency of peracetic acid, uv and ozone after enhanced primary treatment of municipal wastewater. | the city of montreal wastewater treatment plant uses enhanced physicochemical processes (ferric and/or alum coagulation) for suspended solids and phosphorus removal. the objective of this study was to assess the ability of peracetic acid (paa), uv, or ozone to inactivate the indicator organisms fecal coliforms, enterococci, ms-2 coliphage, or clostridium perfringens in the effluent from this plant. paa doses to reach the target fecal coliform level of 9000 cfu/100ml exceeded 6 mg/l; similar resu ... | 2003 | 14568042 |
| chloramphenicol-resistant neisseria meningitidis containing catp isolated in australia. | earlier workers have described chloramphenicol resistance in meningococci isolated from cerebrospinal fluid sampled in patients in vietnam (11 cases) and france (one case) during 1987-1996. here we describe two distinct serogroup b strains isolated in australia in 1994 and 1997, and found among approximately 1400 invasive meningococcal isolates examined in australia over a 9 year period. both were phenotypically chloramphenicol resistant on disc, etest and agar inclusion mic and acetylated chlor ... | 2003 | 14563894 |
| beneficial effect of beta-mannanase feed enzyme on performance of chicks challenged with eimerla sp. and clostridium perfringens. | two experiments were conducted to determine the effect of a beta-mannanase feed enzyme on the performance of broiler chicks subject to a necrotic enteritis disease challenge model involving oral inoculation of eimeria sp. and clostridium pefringens. beta-mannanase is known to improve productive performance when added to poultry and swine diets. in both experiments, disease challenge in the absence of feed additives demonstrated significant reductions in performance as measured by weight gain, fe ... | 2003 | 14562909 |
| incidence and tracking of clostridium perfringens through an integrated broiler chicken operation. | clostridium perfringens has been shown to be widespread in the broiler chicken hatchery, grow-out, and processing operations. in a previous study, ribotypes of certain strains of c. perfringens isolated from processed chicken carcasses were shown to match ribotypes isolated from paper pad lining trays used to transport commercial chicks from the hatchery to the grow-out facility on the farm. these results suggest that c. perfringens contaminating the processed product could originate from facili ... | 2003 | 14562900 |
| claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer. | claudin-4 has been identified as an integral constituent of tight junctions and has been found to be highly expressed in pancreatic cancer. the aim of the present study was to elucidate the effect of claudin-4 on growth and metastatic potential in pancreatic cancer cells, as well as the regulation of claudin-4 by oncogenic pathways. claudin-4 was stably overexpressed in suit-2 pancreatic cancer cells, and its effect on invasion and growth in vitro was examined by using two-chamber invasion assay ... | 2003 | 14559813 |
| bacterial-associated diarrhea in the dog: a critical appraisal. | the clinical documentation of enteropathogenic bacteria causing diarrhea in dogs is clouded by the presence of many of these organisms existing as normal constituents of the indigenous intestinal flora. the diagnosis of a putative bacterial enteropathogen(s) in dogs should be made based on a combination of parameters, including signalment and predisposing factors, clinical signs, serologic assays for toxins, fecal culture, and pcr. relying on results of fecal culture alone is problematic, becaus ... | 2003 | 14552160 |
| genotyping of clostridium perfringens isolated from domestic and exotic ruminants and swine. | clostridium perfringens types a, b, c, d and e are known to cause severe enteritis/enterotoxaemia and diseases (especially caused by type a) belonging to the gas oedema complex in many species. samples from the small intestine as well as faeces of domestic and exotic animals suffering from enterotoxaemic signs or having died within days after first occurance of toxaemia were submitted for typing c. perfringens toxovars by multiplex pcr. the following species have been investigated: domestic shee ... | 2003 | 14535937 |
| tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. | tight junctions (tjs), hallmark structures of one-layered epithelia and of endothelia, are of central biological importance as intramembranous "fences" and as hydrophobic "barriers" between lumina represented by liquid- or gas-filled spaces on the one hand and the mesenchymal space on the other. they have long been thought to be absent from stratified epithelia. recently, however, constitutive tj proteins and tj-related structures have also been identified in squamous stratified epithelia, inclu ... | 2003 | 14533737 |
| dynamic computer simulation of clostridium perfringens growth in cooked ground beef. | the objective of this study was to develop a computer simulation algorithm to dynamically estimate and predict the growth of clostridium perfringens in cooked ground beef. the computational algorithm was based on the implicit form of the gompertz model, the growth kinetics of c. perfringens in cooked ground beef, and the fourth-order runge-kutta numerical method. this algorithm was validated using a cocktail of three strains of c. perfringens spores grown under isothermal, square-waved, linear c ... | 2003 | 14527794 |
| the skhr motif is required for biological function of the virr response regulator from clostridium perfringens. | the response regulator virr and its cognate sensor histidine kinase, virs, are responsible for toxin gene regulation in the human pathogen clostridium perfringens. the c-terminal domain of virr (virrc) contains the functional fxrxhrs motif, which is involved in dna binding and is conserved in many regulatory proteins. virrc was cloned, purified, and shown by in vivo and in vitro studies to comprise an independent dna binding domain. random and site-directed mutagenesis was used to identify furth ... | 2003 | 14526034 |
| diagnosing clostridium perfringens-associated necrotic enteritis in broiler flocks by an immunoglobulin g anti-alpha-toxin enzyme-linked immunosorbent assay. | the tools available for monitoring necrotic enteritis caused by clostridium perfringens in broiler chickens have been limited, particularly for identifying subclinical disease. in this study, a modified enzyme-linked immunosorbent assay was used to quantify levels of specific immunoglobulin g to c. perfringens alpha-toxin in serum from broilers. we found significantly higher antibody levels in broilers with a history of subclinical necrotic enteritis compared with a zinc-bacitracin-treated group ... | 2003 | 14522709 |
| distribution of potential type ii restriction sites (palindromes) in prokaryotes. | restriction-modification systems are used as a defensive mechanism against inappropriate invasion of foreign dna. the recognition sequences for the common type ii restriction enzymes and their corresponding methylases are usually palindromes. in this study, we identified the most over- and underrepresented words in dna of four bacteria: escherichia coli, bacillus subtilis, clostridium perfringens, and pseudomonas aeruginosa. using maximum order markov chain analysis, we found that palindromic wo ... | 2003 | 14521907 |
| do pancreatic duct stents cause or prevent pancreatic sepsis? | pancreatic sepsis can occur after contrast injection into an obstructed or disrupted pancreatic duct. whether stents cause or prevent pancreatic sepsis is unknown. accordingly, the pancreatic duct bacteriology in patients with pancreatic duct stents was retrospectively reviewed and contrasted with biliary cultures taken from patients at the time of bile duct stent retrieval and/or exchange. | 2003 | 14520281 |
| diacylglycerol-rich domain formation in giant stearoyl-oleoyl phosphatidylcholine vesicles driven by phospholipase c activity. | we have studied the effect of phospholipase c from bacillus cereus and clostridium perfringens (alpha-toxin) on giant stearoyl-oleoyl phosphatidylcholine (sopc) vesicles. enzyme activity leads to a binary mixture of sopc and the diacylglycerol sog, which phase separates into a sopc-rich bilayer phase and a sog-rich isotropic bulk-like domain embedded within the membrane, as seen directly by phase contrast microscopy. after prolonged enzymatic attack, all bilayer membranes are transformed into an ... | 2003 | 14507699 |
| effects of tylosin on bacterial mucolysis, clostridium perfringens colonization, and intestinal barrier function in a chick model of necrotic enteritis. | necrotic enteritis (ne) is a worldwide poultry disease caused by the alpha toxin-producing bacterium clostridium perfringens. disease risk factors include concurrent coccidial infection and the dietary use of cereal grains high in nonstarch polysaccharides (nsp), such as wheat, barley, rye, and oats. outbreaks of ne can be prevented or treated by the use of in-feed antibiotics. however, the current debate regarding the prophylactic use of antibiotics in animal diets necessitates a better underst ... | 2003 | 14506046 |
| influence of nacl content and cooling rate on outgrowth of clostridium perfringens spores in cooked ham and beef. | the effect of nacl concentration and cooling rate on the ability of clostridium perfringens to grow from spore inocula was studied with the use of a process that simulates the industrial cooking and cooling of smoked boneless ham and beef roasts. nacl was added to ground cooked hams a and b (which were commercially obtained) to obtain levels of 2.4, 3.1, 3.6, and 4.1% (wt/wt) and 2.8, 3.3, 3.8, and 4.3% (wt/wt), respectively, and to raw ground beef to obtain levels of 0, 1, 2, 3, and 4% (wt/wt). ... | 2003 | 14503712 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
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