Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| infection and immunoglobulin concentrations in chediak-higashi mice. | the chediak-higashi syndrome (chs) has been reported in man, cattle, mink, and mice. chs humans and cattle have an increased incidence of pyogenic infections, whereas chs mink are more susceptible to aleutian disease. age- and sex-matched groups of chs mice (mutant strain) and c57 bl/6n (parent strain) were challenged with candida albicans, escherichia coli, klebsiella pneumoniae, and staphylococcus aureus intravenously and streptococcus pneumoniae intraperitoneally. a significant increase (p < ... | 1974 | 4601767 |
| the kinetics of hydrolysis of synthetic glucuronic esters and glucuronic ethers by bovine liver and escherichia coli beta-glucuronidase. | 1. the relative rates of hydrolysis of synthetically prepared beta-d-glucuronic esters [aglycone: benzoic acid, veratroic (3,4-dimethoxybenzoic) acid, indol-3-ylacetic acid and ethylbutyric acid], and beta-d-glucuronic ethers (aglycone: phenolphthalein, p-nitrophenol, 3,4-dimethoxyphenol, 3,4-dimethoxybenzyl alcohol) by commercial preparations of beta-glucuronidase from bovine liver and escherichia coli were investigated. the rates of hydrolysis of all compounds tested were followed by measuring ... | 1973 | 4584027 |
| [studies, in the field, of a polyvalent vaccine against pulmonary infections in cattle]. | 1972 | 4573942 | |
| transcription of brain chromatin by ribonucleic acid polymerases from brain nuclei and from escherichia coli. | 1. transcription of ox brain chromatin by brain nuclear rna polymerase ii and escherichia coli rna polymerase was studied. 2. the soluble chromatin prepared from brain nuclei contained dna, rna, histone and non-histone proteins. such chromatin preparations did not display any endogenous rna polymerase activity, when assayed in the presence of concentrations of kcl as high as 0.4m. 3. the chromatin-templated activity of brain nuclear polymerase ii was stimulated by kcl, with an optimum around 0.2 ... | 1972 | 4571299 |
| [practical experiences in the prevention and therapy of most important calf and young cattle diseases in large herds]. | 1972 | 4569838 | |
| anti-gamma globulins and chronic infection: comparative studies of the immune response to various bacteria and gamma globulin preparations. | a study of the relationship of clinical states associated with prolonged infection (bacterial endocarditis and osteomyelitis) and generation of serum anti-gamma globulins was made with particular reference to quantitative amounts of staphylococcal protein a in various infecting strains. no correlation between individual strain amounts of protein a and presence of anti-gamma globulins was detected. thirty-eight rabbits were immunized intravenously with various strains of bacteria (staphylococcus ... | 1972 | 4404684 |
| invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a neurospora mutant and a component of molybdenum-enzymes. | an active neurospora-like assimilatory nadph-nitrate reductase (ec 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced neurospora crassa mutant nit-1 with extracts of (a) certain other nonallelic nitrate reductase mutants, (b) uninduced wild type, or (c) xanthine oxidizing and liver aldehyde-oxidase systems was also formed by combination of the nit-1 extract with other acid-treated enzymes known to contain molybdenum. these molybdenum enzymes included (a) nitrogen ... | 1971 | 4399835 |
| degradation of dna rna hybrids by ribonuclease h and dna polymerases of cellular and viral origin. | ribonuclease h from human kb cells, chick embryos, calf thymus, avian myeloblastosis virus, and rous associated virus specifically degrades the rna of dna.rna hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. the cellular rnase h is an endonuclease, whereas the viral enzyme appears to be an exonuclease. viral dna polymerase and rnase h copurify through all separation steps. therefore, rnase h activity is an intrinsic part of the viral dna polymerase. dna.rna hybrids ... | 1972 | 4343966 |
| electrophoretic analysis of ribosomal and viral ribonucleic acids with a simple technique for slicing low-concentration polyacrylamide gels. | the electrophoretic mobilities of ribosomal ribonucleic acids (rna) from cultured mammalian (hela, vero, mdbk), avian (chick embryo), and bacterial (escherichia coli) cells, and rna species extracted from selected viruses (sindbis, polio, tobacco mosaic, sendai) were compared, employing a simple, inexpensive technique for slicing low-concentration polyacrylamide gels. the procedure provides for rapid fractionation of gels used for characterization of rna, incorporating extrusion and serial secti ... | 1971 | 4331767 |
| the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service. | the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ... | 1971 | 4326247 |
| studies on the chick-lethal toxin of eecherichia coli. | a toxin which is lethal for two week old chicks has been recovered from strains of escherichia coli o78:k80 of bovine and avian origin and from avian isolates of serogroups o2, o45 and o109. the toxin is heat-labile, antigenic, high in protein, inactivated by pronase, trypsin, amylase, and pancreatic lipase. the toxin may be precipitated by ammonium sulfate or tca treatment from the supernatant obtained by repeated centrifugation of sonicated cells. considerable purification has been obtained by ... | 1973 | 4270809 |
| [r factors in strains of pathogenic enterobacteria isolated from domestic animals and particularly from dogs]. | strains of enterobacteria (nine escherichia coli and two salmonella) isolated from primary or secondary infections in the dog, cat, pig, calf and kangaroo were studied for the presence of extrachromosomal drug resistance factors (r factors). seven strains of e. coli and two strains of salmonella transferred resistance involving the following antibiotics: streptomycin, ampicillin, chloramphenicol, neomycin and tetracycline. all strains harboring r factors transferred streptomycin resistance and t ... | 1972 | 4258542 |
| escherichia coli agglutinins in cow serum, colostrum and the nursing calf. | immunochemical properties of escherichia coli o antibodies present in bovine serum and colostrum were investigated. dam and calf serum samples plus colostral whey samples were fractionated by gel filtration, and the 7s and 19s fractions isolated. antibody activity against the o antigens of four recognized e. coli bovine pathogens was determined by the indirect hemagglutination test on the whole serum and colostral whey samples and the 7s and 19s fractions thereof. mercaptoethanol reduction was u ... | 1970 | 4248443 |
| herd incidence of bovine mastitis in four danish dairy districts. i. the prevalence and mastitogenic effect of micro-organisms in the mammary glands of cows. | 1974 | 4213559 | |
| modification of rna polymerase after t3 phage infection of escherichia coli b. | e. coli b cells infected with t3 phage contain a modified host rna polymerase in addition to the normal rna polymerase found in uninfected cells. the modified rna polymerase behaves differently in its elution properties from the normal enzyme on deae-cellulose, phosphocellulose, and dna-cellulose column chromatography. the modified enzyme also differs from the normal polymerase in some of its enzymatic parameters. the specific activity of the modified rna polymerase is markedly lower (i.e., (1/4 ... | 1973 | 4126728 |
| a technique for ultracryotomy of cell suspensions and tissues. | ultracryotomy of fixed tissue has been investigated for a number of years but, so far, success has been limited for several reasons. the simple technique herein reported allows the ultracryotomy not only of a variety of tissues but also of single cells in suspension, with a preservation and visualization of ultrastructural detail at least equivalent to that obtained with conventional embedding procedures. in this technique, sucrose is infused into glutaraldehyde-fixed tissue pieces before freezi ... | 1973 | 4121290 |
| antigenic relationships in mammalian dna polymerase. | rabbit antibody was prepared against a high-molecular-weight dna polymerase purified from the soluble fraction of calf thymus gland. this antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight dna polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse l cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone ... | 1972 | 4110484 |
| the influence of murine macrophage-conditioned medium on cloning efficiency, antibody synthesis, and growth rate of hybridomas. | murine b-cell hybridomas made with the p3x63-ag8.653 myeloma showed increases in cloning efficiency and efficiency of growth in hypoxanthine-aminopterin-thymidine (hat) medium of 50-100-fold in the presence of medium conditioned by primary mouse peritoneal macrophages (mcm). similar effects were elicited by mcm from 3 continuous macrophage lines. the j774a.1 line conditioned the medium as efficiently as primary macrophages without induction. conditioning by the p388d1 line was several-fold less ... | 1985 | 3998479 |
| clinical pharmacology of apramycin in calves. | the minimal inhibitory concentrations (mic) of apramycin, a unique aminocyclitol antibiotic, were compared with the mic of dihydrostreptomycin and neomycin for 323 salmonella, 178 escherichia coli and twenty-six pasteurella multocida isolates recovered from newborn calves. apramycin exhibited better in vitro anti-bacterial activity than dihydrostreptomycin and neomycin; isolates of salmonella group b and e. coli resistant to the latter were sensitive to apramycin. the two-compartment open model ... | 1985 | 3989905 |
| beta-sulfur substituted alpha-ketoglutarates as inhibitors and alternate substrates for isocitrate dehydrogenases and certain other enzymes. | the rs-isomers of beta-mercapto-alpha-ketoglutarate, beta-methylmercapto-alpha-ketoglutarate and beta-methylmercapto-alpha-hydroxyglutarate have been synthesized. beta-mercapto-alpha-ketoglutarate was a potent inhibitor, competitive with isocitrate and noncompetitive with nadp+, of the mitochondrial nadp-specific isozyme from pig heart (ki = 5 nm; km (dl-isocitrate)/ki(rs-beta-mercapto-alpha-ketoglutarate) = 650) and pig liver, the cytosolic isozyme from pig liver (i0.5 = 23 nm), and the nadp-li ... | 1986 | 3947094 |
| arachidonic acid metabolites in milk of cows during acute coliform mastitis. | concentrations of prostaglandin f2 alpha (pgf2 alpha) and thromboxane b2 (txb2) were evaluated in the milk of cows with naturally occurring (n = 3) and experimentally induced (n = 5) acute coliform mastitis. these arachidonic acid metabolites were measured by radioimmunoassay in unextracted milk. experimental infections were induced by inoculating 600 to 1,200 colony-forming units of escherichia coli into 1 mammary quarter per experimental cow. in the experimental cows, milk was collected before ... | 1985 | 3927804 |
| examination of calf prochymosin accumulation in escherichia coli: disulphide linkages are a structural component of prochymosin-containing inclusion bodies. | recent reports have shown that synthesis of certain recombinant proteins in escherichia coli results in the production of intracellular inclusion bodies. these studies have not analyzed the structure of the inclusion body especially regarding the intermolecular forces holding it together. we have examined structural aspects of inclusion bodies made in e. coli as a result of high level expression of the eukaryotic protein, calf prochymosin. prochymosin is a monomeric protein containing three disu ... | 1985 | 3924595 |
| [characterization of strains of escherichia coli isolated from calves with neonatal diarrhea]. | thirty fecal samples of diarrheic calves from a beef herd with previous history of neonatal diarrhea were cultured for isolation of escherichia coli k99 (+) and for enterotoxigenic tests. the age distribution of sampled calves was: 1 animal less than 6 days old, 21 between 7-15 days old and 8 between 16-30 days old. although most strains were classified as isaacson et al (16) biotype four, they were negative for detection of k99 antigen by slide agglutination test. the assay for heat-stable (st) ... | 1985 | 3916646 |
| effect of iron on serotypes and haemagglutination patterns of escherichia coli in bottle-fed infants. | strains of escherichia coli isolated from faecal specimens of ten infants receiving breast milk, six receiving a cow-milk preparation with iron supplement (5 mg/l) and six the preparation without iron supplement (less than 0.5 mg/l), were serotyped and examined for their haemagglutinating activity. the escherichia coli flora of breast-fed and bottle-fed infants consisted of one resident strain, accompanied by one or more transient strains. changes in the serotype of the escherichia coli flora an ... | 1985 | 3912179 |
| septicemic colibacillosis and failure of passive transfer of colostral immunoglobulin in calves. | septicemic colibacillosis is a highly fatal disease that occurs in calves less than 2 weeks of age. the disease occurs when a calf that fails to absorb protective levels of immunoglobulin from colostrum is exposed to an invasive serotype of e. coli. management to ensure good passive transfer of colostral immunoglobulin will prevent this disease and reduce calf mortality caused by other infectious diseases as well. | 1985 | 3907781 |
| synthesis and biochemical characterization of a photoactivatable, iodinatable, cleavable bacterial lipopolysaccharide derivative. | a method for the synthesis of a photoactivatable, iodinatable, and thiol-cleavable derivative of bacterial lipopolysaccharide (lps) is described using sulfosuccinimidyl 2-(p-azidosalicylamido)-1,3'-dithiopropionate. the method described is applicable to lps from both smooth and rough bacteria. evidence is presented that the coupling reaction occurs primarily to phosphoethanolamine residues localized to the inner core region of the lps. radioiodination of the derivatized lps results in a product ... | 1985 | 3905790 |
| arabinosylnucleoside 5'-triphosphate inhibits dna primase of calf thymus. | it has been shown that dna primase activity is tightly associated with 10s dna polymerase alpha from calf thymus and that the ribonucleotide-dependent dna synthesis is more sensitive to aractp than dna-primed dna synthesis (yoshida, s., et al. (1983) biochim. biophys. acta 741, 348-357). here we measured dna primase activity using poly(dt) template or m13 bacteriophage single-stranded dna template and primer rna synthesis was coupled to the reaction by escherichia coli dna polymerase i klenow fr ... | 1985 | 3905783 |
| cloning and expression of the leukotoxin gene of pasteurella haemolytica a1 in escherichia coli k-12. | a clone bank of pasteurella haemolytica a1 was constructed by partial digestion of the genomic dna with sau3a and ligation of 5- to 10-kilobase-pair fragments into the bamhi site of the plasmid vector pbr322. after transformation into escherichia coli k-12, a total of 4 x 10(3) recombinant clones was obtained. these were screened for the production of p. haemolytica soluble antigens by a colony enzyme-linked immunosorbent assay blot method with a rabbit antiserum raised against the soluble antig ... | 1985 | 3905610 |
| single amino acid substitutions at conserved residues of human interferon-alpha can effect antiviral specific activity. | site-specific in vitro mutagenesis was used to direct various amino acid substitutions at conserved positions within the sequence of human interferon-alpha 1 (ifn-alpha 1). the antiviral specific activity of ifn-alpha 1, expressed in m13 as a fusion protein [ifn-alpha 1 (phi wt)], could be altered by single amino acid substitutions. the substitution of glycine for tyrosine at position 123 results in a loss of more than 99% of the antiviral specific activity on human cells, but causes no signific ... | 1985 | 3904757 |
| induction of dna strand breaks by rsu-1069, a nitroimidazole-aziridine radiosensitizer. role of binding of both unreduced and radiation-reduced forms to dna, in vitro. | [2-14c]-rsu-1069 [1-(2-nitro-1-imidazolyl)-3-(1-aziridino)-2-propanol], either as a parent (unreduced) or following radiation reduction, binds to calf thymus dna in vitro. radiation-reduced rsu-1069 binds to a greater extent and more rapidly than the parent compound. rsu-1137, a nonaziridino analogue of rsu-1069, binds following radiation reduction. radiation-reduced misonidazole (1-(2-nitro-1-imidazolyl)-3-methoxy-2-propanol) exhibits binding ratios a thousand-fold less than those of reduced rs ... | 1985 | 3902026 |
| [identification of bacterial clones that encode cow's caseins by direct immunological screening of the cdna library]. | a sensitive immunoassay was used to identify recombinant dna plasmids carrying cdna fragments of bovine caseins in the cdna library from rrna of bovine mammary glands. colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto cnbr-activated filter paper. antigens covalently bound to the cnbr-activated paper or bound to the nitrocellulose filters were detected by reaction with antiserum to caseins, followed by 125i-labeled protein a from staphyloc ... | 1985 | 3900695 |
| cloning of a bovine interferon-alpha gene subfamily and comparisons between genetically engineered and leukocyte bovine interferons. | bovine peripheral leukocytes were virally induced for interferon production, and an acid stable, sds stable, antiviral activity was detected in the preparation. this bovine interferon (boifn) was tested for its ability to induce an antiviral state in various mammalian cells and was found to be specific to cells from bovine origin. the boifn cross reacts with antibodies against human ifn-alpha but these antibodies do not neutralize the bovine ifn activity. leukocyte boifn exhibits polymorphism up ... | 1985 | 3899795 |
| [enzyme constellation of the blood plasma in the prognosis of decreased adaptability in newborn calves]. | the dynamic was studied of the enzyme activity of che, aph, ald, goat, gpt, ldg, and cph in 30 newborn calves. fifteen of the animals developed coli bacteriosis later on. blood was sampled at the 6th to 24th hour as well as on the 5th day following birth. at the 6th hour che, aph, ald, and cph of the diseased calves showed lower activity as against the enzyme activity of the unaffected animals. similar trend was noticed also at the 24th hour. contrary to the other enzymes of the constellation th ... | 1985 | 3898561 |
| in vivo proteolytic activity of the mammary gland. contribution to the origin of secretory component, beta 2-microglobulin and bovine-associated mucoprotein (bamp) in cows milk. | milk samples were collected from holstein-friesian cows at various times after milking (10-30 min; 30 min-10 hr) and treated with a protease inhibitor or control solution. samples were then fractionated into whole, skimmed and cell-free skimmed milk aliquots. some animals were treated with e. coli endotoxin prior to sample collection. the concentrations of three membrane-associated proteins (map), beta 2-microglobulin (beta 2m), secretory component (sc) and bovine-associated mucoprotein (bamp) a ... | 1985 | 3898552 |
| effect of prepartum vaccination with k99 escherichia coli vaccine on maternal and calf blood antibody concentration and calf health. | one hundred and two dry, pregnant holstein cows were identified alternately as vaccinated or nonvaccinated (group 0) animals. vaccinated cows were scheduled for vaccination at 6 and 3 wk prior to expected calving date with vicogen, a commercial vaccine produced for the prevention of calf scours caused by enteropathogenic escherichia coli that possess the k99 antigen. group 1 included cows that were less than 6 wk from freshening when the experiment started and, therefore, received only one vacci ... | 1985 | 3897306 |
| chorismate mutase-prephenate dehydrogenase from escherichia coli: cooperative effects and inhibition by l-tyrosine. | the effects of a variety of structural analogs of l-tyrosine on the mutase and dehydrogenase activities of hydroxyphenylpyruvate synthase have been investigated. from these studies it is concluded that the alpha-nh3+ alpha-coo-, and the 4-oh groups are essential for binding of l-tyrosine as an inhibitor of the dehydrogenase and that the l configuration is also essential. dixon plots for inhibition of the dehydrogenase activity by some of these analogs were nonlinear and could be described by a v ... | 1985 | 3896148 |
| efficient expression in escherichia coli of two species of human interferon-alpha and their hybrid molecules. | a new type of interferon (ifn)-alpha cdna (ifn-alpha i') was identified in a cdna library constructed from namalva cells infected with sendai virus. the nucleotide sequence of this cdna showed homology, with the exception of two nucleotides in the coding region, with the previously identified ifn-alpha i gene (lawn et al., 1981). an expression plasmid which directs the synthesis of the mature ifn-alpha i' peptide was constructed using vectors carrying the lpp/lac promoter and "runaway" replicon. ... | 1985 | 3891272 |
| characterization of the coliform and enteric bacilli in the environment of calves with colibacillosis. | in the first part of the present study the coliform and enteric bacilli in the environment of calves with colibacillosis were examined. the occurrence, number, and pathogenic properties of escherichia coli in barnyard soils were obtained from six cattle ranches. the o and k serogroups of e. coli isolates obtained from the feces of calves with colibacillosis born at these cattle ranches were determined, and their serotypes were compared with the e. coli o and k serotypes found in soils. the resul ... | 1985 | 3890744 |
| trna-rrna sequence homologies: evidence for an ancient modular format shared by trnas and rrnas. | homologies between trnas and rrnas are identified in searches using various combinations of escherichia coli, yeast, halobacterium volcanii and bovine mitochondrial sequences. as in previously reported comparisons, the homologies are too frequent and long to be attributed to coincidence, and similar frequencies from inter- and intraspecies comparisons preclude evolutionary convergence as an explanation. in contrast to the earlier studies, patterns in the positioning of the homologies are now des ... | 1985 | 3888302 |
| linkage of genes for heat-stable enterotoxin, drug resistance, k99 antigen, and colicin in bovine and porcine strains of enterotoxigenic escherichia coli. | linkages among genes for production of escherichia coli heat-stable enterotoxin (st), drug resistance, k99 antigen, and colicin were investigated in 2 bovine and 3 porcine strains of enterotoxigenic e coli. in conjugation experiments, all 5 isolates transferred enterotoxigenicity and the ability to produce k99; 4 of the 5 isolates also transferred antibiotic resistance markers, and 3 colicinogenic strains transmitted the ability to produce colicin. in 2 of the 3 colicin-producing strains, the ge ... | 1985 | 3888010 |
| rapid kinetics of ca2+-induced fusion of phosphatidylserine/phosphatidylethanolamine vesicles. the effect of bilayer curvature on leakage. | we have employed both small unilamellar vesicles (suv) and large unilamellar vesicles formed by the reverse phase evaporation technique (rev) to study the initial kinetics of membrane aggregation and fusion. stopped flow measurements of the calcium-induced changes in the turbidity of suv and rev, formed from 1:1 (mol/mol) mixtures of bovine phosphatidylserine (ps) and escherichia coli phosphatidylethanolamine (pe), were used to follow particle aggregation. simultaneous measurements of the fluore ... | 1985 | 3884605 |
| the ecology of escherichia coli in calves reared as dairy-cow replacements. | a continual turn-over in the strains forming the majority of faecal escherichia coli flora was demonstrated in 16 calves reared as dairy cow replacements. the incidence of antibiotic resistance among isolates, as measured by an antibiotic resistance index (ari), changed markedly with the age of the calf. the value was low initially, when the calves were 1-2 days old and housed with adult animals. it then rose rapidly during the first week after the animals had been weaned and moved into nursery ... | 1985 | 3884563 |
| an assessment of bacteroides fragilis group organisms as indicators of human faecal pollution. | membrane filtration techniques were used to enumerate bacteroides fragilis group (bfg) organisms and escherichia coli in a variety of natural waters, the influents and effluents from three types of sewage treatment plants and faeces of various animals. the results suggest that bfg organisms die off more rapidly than e. coli in water and that animal faeces are not a significant source of bfg. it is suggested that the ratio of bfg to e. coli in water may be used to indicate the proximity of a sour ... | 1985 | 3884562 |
| the ecology of escherichia coli in market calves fed a milk-substitute diet. | dynamic changes in the escherichia coli population in the calf gut were studied over 21 days in a group of 18 intensively-reared market calves. isolates were identified by o-serogrouping, biotyping and resistogram patterns. seventy o-serogroups were identified among nearly 3000 e. coli isolates examined and these were subdivided into 416 strains by means of their biotype and resistogram. seventy-five per cent of these strains were detected only once or twice, which points to the continual replac ... | 1985 | 3884561 |
| channeling of a beta-oxidation intermediate on the large subunit of the fatty acid oxidation complex from escherichia coli. | the kinetic properties of the fatty acid oxidation complex from escherichia coli were studied with the aim of elucidating the functional consequence of having enoyl-coa hydratase and 3-hydroxyacyl-coa dehydrogenase associated with a multifunctional polypeptide. the kinetic parameters of individual enzymes were determined and used in model calculations based on a published theory (storer, a. c., and cornish-bowden, a. (1974) biochem. j. 141, 205-209) to predict the kinetic behavior of a system of ... | 1985 | 3882701 |
| production of neutralizing monoclonal antibodies to escherichia coli heat-stable enterotoxin. | in an effort to develop new approaches to the study and control of infectious diarrhea, we prepared murine monoclonal antibodies to the escherichia coli heat-stable enterotoxin (sta). the toxin was purified from e. coli culture media and conjugated to bovine serum albumin. the sta-bovine serum albumin conjugate was used to immunize balb/c mice, and the immune spleen cells from these mice were fused with sp2/0 myeloma cells. resultant hybridomas were screened in an enzyme-linked immunosorbent ass ... | 1985 | 3880723 |
| c1q, a subunit of the first component of complement, enhances binding of plasma fibronectin to bacteria. | the interaction of plasma fibronectin with c1q of the complement system has been demonstrated in the past several years. in addition, the antibody-independent binding of c1q to bacteria, as well as the binding of plasma fibronectin to bacteria, is well documented. this study examines whether the binding of c1q to bacteria enhances the interaction of c1q and bacteria with plasma fibronectin. highly purified 125i-c1q bound to several species of bacteria in the absence of antibody. the binding of 1 ... | 1985 | 3875560 |
| cryptosporidium among children with acute diarrhoea in the west of ireland. | during a period of 12 months beginning on 18 february 1985, 1246 specimens of faeces from 935 children aged 3 weeks to 12 years with acute diarrhoea of varying severity were examined for the presence of cryptosporidium oocysts. twenty-six patients required admission to hospital; four of them needed intravenous rehydration. predominant clinical features were diarrhoea, often watery, and vomiting. diarrhoea varied in duration between 7-15 days. cryptosporidium oocysts were found in specimens from ... | 1987 | 3819459 |
| [etiology and chemotherapy of suppurative surgical infection in horses and cattle]. | a total of 24 horses and 42 cattle with a variety of local suppurative processes (wounds, abscesses, phlegmons, tendovaginitis, etc.) were studied under conditions of the clinic with regard to the most commonly found microbial species and the opportunity to work out antibioticogrammes in terms of the effectiveness of 11 of the chemotherapeutic agents widely used in the practice. it was found that prevailing in horses were staphylococci and streptococci. in cattle corynebacterium pyogenes was pre ... | 1986 | 3811221 |
| dexamethasone-induced attenuation of cardiopulmonary dysfunction in endotoxemic calves. | effects of dexamethasone on pulmonary hemodynamics, pulmonary mechanics, and gas exchange were determined in anesthetized (pentobarbital sodium) and paralyzed (pancuronium bromide) calves (9.4 +/- 0.4 weeks old) during 5 hours of endotoxemia. escherichia coli endotoxin (055-b5) was infused iv at 20 micrograms/kg the 1st hour, followed by a continuous infusion at 10 micrograms/kg/hour for the following 4 hours. dexamethasone (5 mg/kg) was given iv 18 hours and 1 hour before endotoxin administrati ... | 1986 | 3777644 |
| aetiology of diarrhoea in young calves. | faeces samples were collected from 302 untreated calves on the day of onset of diarrhoea and from 49 healthy calves at 32 farms experiencing outbreaks of diarrhoea. at least four diarrhoeic calves were sampled on each farm, and samples were examined for rotavirus, coronavirus, cryptosporidium, enterotoxigenic escherichia coli and salmonella species. although all these enteropathogens were excreted more frequently by the diarrhoeic than by the healthy calves, the difference was significant overal ... | 1986 | 3750766 |
| modification of bovine heart succinate dehydrogenase with ethoxyformic anhydride and rose bengal: evidence for essential histidyl residues protectable by substrates. | purified and membrane-bound succinate dehydrogenase (sdh) from bovine heart mitochondria was inhibited by the histidine-modifying reagents ethoxyformic anhydride (efa) and rose bengal in the presence of light. succinate and competitive inhibitors protected against inhibition, and decreased the number of histidyl residues modified by efa. the essential residue modified by efa was not the essential thiol of sdh, but modification of the essential thiol abolished the protective effect of malonate ag ... | 1986 | 3717948 |
| human and bovine milk: comparison of ganglioside composition and enterotoxin-inhibitory activity. | milk gangliosides inhibit vibrio cholerae enterotoxin and escherichia coli heat-labile enterotoxin. human milk gangliosides showed considerably higher enterotoxin-inhibitory activity compared to bovine and formula milk gangliosides as measured in vitro by enzyme-linked immunosorbent assay and in vivo in rabbit small bowel loops. while gangliosides from less than 1 ml human milk inhibited 0.1 microgram choleratoxin in vitro and in vivo, five to 10 times higher amounts of bovine milk gangliosides ... | 1986 | 3714350 |
| expression of a pseudogene for interferon-alpha l. | the human leukocyte interferon-alpha l (ifn-alpha l) appears to be a pseudogene because it contains a termination codon in the dna coding for the precursor peptide (signal peptide), but the remainder of the gene codes for an interferon protein of normal length. to determine if this gene codes for an active interferon molecule, an expression plasmid was constructed for ifn-alpha l that was produced in escherichia coli. the ifn-alpha l is active on human and bovine cells, and exhibits a trace of a ... | 1987 | 3681016 |
| specificity of o6-alkylguanine-dna alkyltransferase. | extensive investigations of the specificity of o6-alkylguanine-dna alkyltransferase (aat) have been carried out. these studies have shown that: (i) the mammalian protein differs from that of escherichia coli in lacking the ability to remove methyl groups from o4-methylthymine; (ii) the protein can remove longer alkyl groups from the o6 position but the rate of repair declines as the chain length increases; (iii) o6-methylguanine in rna is much less active as a substrate for the protein than o6-m ... | 1987 | 3679391 |
| characterization of tryptophan environments in glutamate dehydrogenases from temperature-dependent phosphorescence. | tryptophan room temperature phosphorescence in solution was detected in glutamic dehydrogenase from bovine liver and escherichia coli with lifetimes of 1.2 and 0.65 s, respectively. although these enzymes possess three and five tryptophanyl residues per polypeptide chain, respectively, the temperature dependence of the phosphorescence quantum yield estimates that the room temperature emission is due, in either case, to a single residue. long triplet-state lifetimes and very small rates of o2 que ... | 1987 | 3663638 |
| campylobacter jejuni in dairy cows and raw milk. | twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ... | 1987 | 3595744 |
| recombinant-dna-derived bovine growth hormone from escherichia coli. 1. demonstration that the hormone is expressed in reduced form, and isolation of the hormone in oxidized, native form. | the isolation of bacterially synthesized, recombinant-dna-derived, bovine growth hormone (r-bgh) with native structure is described. the r-bgh is found in insoluble form, in a pellet fraction, after cell breakage and centrifugation. cell envelope components (protein, lipid, endotoxin) and nucleic acids are selectively removed from the pellet fraction by an edta/lysozyme/deoxycholate extraction. we demonstrate that the r-bgh is largely reduced until solubilized using 6 m guanidine/hcl. air oxidat ... | 1987 | 3545831 |
| [possibility of fortifying milk nutrient mixtures for children with lactoglobulin against e. coli and proteus]. | sweet milk nutrient mixtures, biologically active additives (baa) and lactic acid ferments, intended for child nutrition, were enriched with the immune preparation lactoglobulin against e. coli and proteus. serological and immunochemical investigations of hemagglutinating activity and other characteristics of lactoglobulin in the enriched milk mixtures and baa evidenced that the preparation retained its full value during the technological process and in the period of storage. no signs of lactogl ... | 1986 | 3541377 |
| apramycin and gentamicin resistance in escherichia coli and salmonellas isolated from farm animals. | since the aminoglycoside antibiotic apramycin was licensed for veterinary use in 1980, all isolates of escherichia coli and salmonellas received at the central veterinary laboratory have been monitored for resistance to apramycin and the related antibiotic gentamicin. during the period 1982-4, the incidence of resistance in e. coli to apramycin increased from 0.6% in 1982 to 2.6% in 1984. in salmonellas the incidence of resistance to apramycin increased from 0.1% in 1982 to 1.4% in 1984. resista ... | 1986 | 3540112 |
| two-site column enzyme immunoassay for neuron-specific enolase (nse) in human serum using monoclonal antibodies. | a new method for the rapid determination of neuron-specific gamma-enolase (nse), gamma-subunit of alpha gamma- and gamma gamma-enolase in human serum was developed by employing monoclonal antibodies for the separation method. the assay system consists of 0.1 ml sepharose 4b column with immobilized rabbit anti-mouse igg antibodies for the separation of bound label, fab' fragments of rabbit anti-bovine gamma gamma-enolase igg labeled with beta-d-galactosidase from escherichia coli, and f(ab')2 fra ... | 1986 | 3537135 |
| cow milk yield and composition before development of escherichia coli mastitis. | high milk yield, low milk fat and low milk protein were considered as possible predisposing factors to bovine escherichia coli mastitis. morning and afternoon milk yields were recorded in 46 friesian cows later developing e coli mastitis and compared with 92 uninfected controls. animals developing e coli mastitis gave a significantly higher milk yield than controls. the overall morning: afternoon ratio was (mean +/- se) 1.66 +/- 0.41, with no difference in ratio for the two groups. further studi ... | 1986 | 3535230 |
| evaluation of selective media for primary isolation of treponema hyodysenteriae and treponema innocens. | a total of 2450 samples of feces, intestinal contents and colon mucosal scrapings were bacteriologically examined. a total of 53 strains of treponema sp. were isolated, and 45 strains of bacteroides sp., 30 strains of e. coli, 30 strains of micrococcus sp. and 10 strains of streptococcus d isolates were randomly selected. growth promoting studies showed statistically significant stimulation of treponema sp. growth by yeast extract, chicken egg yolk and rumen fluid. different growth inhibitors we ... | 1986 | 3533408 |
| hemolytic and bactericidal activities of bovine complement in mammary secretions of cows during the early nonlactating (dry) period. | hemolytic and bactericidal complement-dependent activities were measured in quarter mammary secretions obtained during the first 21 days of the nonlactating (dry) period from 8 holstein-friesian cows. we demonstrated an inhibition of hemolytic activity and bactericidal activity against a serum-sensitive escherichia coli strain. both hemolytic and inhibitory titers increased markedly during active involution. the bactericidal activity of dry secretions required a minimal threshold of complement a ... | 1986 | 3532874 |
| aerobic blood culturing in cows with coliform mastitis. | aerobic culturing was performed on blood samples from 20 cows with coliform mastitis and signs of systemic illness, and on blood samples from 21 cows with mastitis caused by other agents. no bacteria other than the skin contaminant bacillus sp were isolated from the blood. | 1986 | 3531122 |
| escherichia coli o157:h7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. | in september 1984, an outbreak of escherichia coli o157:h7 infection occurred in a nursing home. it was the first recognized outbreak of this organism in the united states since 1982, when two outbreaks led to its recognition as a pathogen. thirty-four of 101 residents developed a diarrheal illness; 14 were hospitalized with a severe illness characterized by crampy abdominal pain, marked abdominal distention, and grossly bloody diarrhea, and four died. the spectrum of illness associated with the ... | 1986 | 3528316 |
| growth of environmental mastitis pathogens in various bedding materials. | the objective of the study was to determine whether, under controlled conditions, bedding materials vary in their ability to support growth of different environmental pathogens independent of the presence of feces, urine, or other contamination. five sterilized bedding materials (fine hardwood chips, recycled dried manure, chopped newspaper, softwood sawdust, and chopped straw) and three bacterial species (escherichia coli, klebsiella pneumoniae, and streptococcus uberis) were used for a total o ... | 1986 | 3528251 |
| 3-hydroxyacyl-coa epimerases of rat liver peroxisomes and escherichia coli function as auxiliary enzymes in the beta-oxidation of polyunsaturated fatty acids. | the beta-oxidation of 2-trans,4-cis-decadienoyl-coa, an assumed metabolite of linoleic acid, by purified enzymes from mitochondria, peroxisomes, and escherichia coli was studied. 2-trans,4-cis-decadienoyl-coa is an extremely poor substrate of the beta-oxidation system reconstituted from mitochondrial enzymes. the results of a kinetic evaluation lead to the conclusion that in mitochondria 2-trans,4-cis-decadienoyl-coa is not directly beta-oxidized, but instead is reduced by nadph-dependent 2,4-di ... | 1986 | 3528151 |
| characterization of heat-stable enterotoxin from a hypertoxigenic escherichia coli strain that is pathogenic for cattle. | an enterotoxigenic escherichia coli (etec) strain isolated from a calf with clinical scours was found to produce over 17- to 60-fold more heat-stable enterotoxin (sta) than four laboratory-adapted bovine etec strains. the purified sta of this strain was identical to those produced by other etec strains. a severe form of scours was induced in 5- to 15-day-old colostrum-fed calves and in 1- to 2-week-old piglets by oral administration of the purified sta. this study demonstrates that sta is a medi ... | 1986 | 3525417 |
| bacteriostatic activity of bovine milk lactoferrin against mastitic bacteria. | the bacteriostatic activity of bovine lactoferrin (lf) against mastitic bacteria was assessed with an in vitro microassay. the most susceptible species was escherichia coli; all of the 35 isolates tested were susceptible to bacteriostasis by apo-lf (0.1 mg ml-1), although a few strains showed a lower degree of inhibition. heterogeneity among strains was more pronounced among 10 isolates of staphylococcus aureus, four of which were apparently unaffected by apo-lf (1 mg ml-1). under the same condi ... | 1986 | 3523963 |
| observations on the incidence of clinical bovine mastitis in non-lactating cows in england and wales. | epidemiological features of clinical mastitis in dry cows from a three year prospective study are described. two hundred and seventy-three herds in england and wales participated in the first year (1980) and 209 and 159 of them continued in the study in the subsequent two years. clinical mastitis was recorded in 1.5 per cent of cows during the dry period in each of the three years. these cases represented between 3.6 and 4.2 per cent of samples from all clinical cases examined. the incidence of ... | 1986 | 3523959 |
| clinical pharmacokinetics of flumequine in calves. | the minimal inhibitory concentration (mic) of flumequine for 249 salmonella, 126 escherichia coli, and 22 pasteurella multocida isolates recovered from clinical cases of neonatal calf diarrhoea, pneumonia and sudden death was less than or equal to 0.78 microgram/ml. the pharmacokinetics of flumequine in calves was investigated after intravenous (i.v.), intramuscular (i.m.) and oral administration. the two-compartment open model was used for the analysis of serum drug concentrations measured afte ... | 1986 | 3522937 |
| emergence of aminoglycoside 3-n-acetyltransferase iv in escherichia coli and salmonella typhimurium isolated from animals in france. | we studied two outbreaks of calf salmonellosis caused by apramycin and gentamicin-resistant salmonella typhimurium strains. in both cases, the responsible strains were resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, tetracycline, and trimethoprim; one strain was also resistant to nalidixic acid in one outbreak. a systematic survey of the intestinal escherichia coli strains of calves from the two affected flocks showed that 11 of 24 animals sampled were also colonized by apramy ... | 1986 | 3521474 |
| a review of teat factors in bovine e coli mastitis. | escherichia coli mastitis was first reproduced in 1903 by sticking the organism to teat orifices. e coli is very common in the environment of housed dairy cows and mastitis can easily be reproduced experimentally by the introduction of as few as 20 organisms into the teat cistern via the teat duct. it is generally accepted that this is the route of natural infection but the processes by which the organisms traverse the teat duct remain unclear. the literature is reviewed and the facts and hypoth ... | 1986 | 3521057 |
| aminoglycoside binding sites in escherichia coli as revealed by neomycin-gold labeling. | a cytochemical technique for demonstration of neomycin binding sites by electron microscopy was developed and applied to escherichia coli. neomycin was conjugated chemically with bovine serum albumin (bsa). colloidal gold was coated with the conjugated neomycin-bsa. the neomycin-bsa-gold was applied to thin sections of epon-embedded e. coli and examined. gold particles were observed on the outer membrane and the cytoplasmic membrane of e. coli. it was probably the ribosomes that were being label ... | 1986 | 3519754 |
| decreased complement and bacteriostatic activities in the sera of cattle given single or multiple doses of aflatoxin. | five steers given 1 dose of partially purified aflatoxin at concentrations sufficient to provide 0.2 mg to 0.8 mg of aflatoxin b1 equivalents/kg of body weight were compared with 4 steers given 14 daily doses of 0.25 mg of aflatoxin b1 equivalents/kg of body weight for complement activity and bacteriostasis. complement activity was measured by hemolysis in gel, and bacteriostatic activity, by growth inhibition of escherichia coli in liquid medium. in the single-dose group, complement activity an ... | 1986 | 3516032 |
| growth inhibition of environmental mastitis pathogens during physiologic transitions of the bovine mammary gland. | ten dairy cows were infused intramammarily near drying off with concanavalin a (cona) or phytohemagglutinin (pha). mammary secretions were collected during physiologic transitions of the udder and were used in an in vitro microbiological assay to determine growth inhibition of mastitis pathogens. as mammary involution progressed, in vitro growth inhibition of klebsiella pneumoniae, escherichia coli, and streptococcus uberis increased. mammary secretions from cona- and pha-treated glands had sign ... | 1986 | 3513675 |
| gastrointestinal plasma leakage in endotoxic shock. inhibition by prostaglandin e2 and by a platelet-activating factor antagonist. | the effects of pretreatment with prostaglandin e2 or the platelet-activating factor antagonist, cv-3988, on endotoxin-induced gastric damage, gastrointestinal plasma protein leakage, and systemic hypotension were examined in the rat. endotoxic shock was induced by intravenous administration of lipopolysaccharide from escherichia coli and was characterized by prolonged hypotension, gastrointestinal hyperemia and hemorrhage, and marked leakage of radiolabelled albumin into the interstitium and lum ... | 1987 | 3478114 |
| amino acid sequence of the nad (h)--binding region of the mitochondrial nicotinamide nucleotide transhydrogenase modified by n,n'-dicyclohexylcarbodiimide. | purified mitochondrial energy-linked nicotinamide nucleotide transhydrogenase (th) is inhibited by n,n'-dicyclohexylcarbodiimide (dccd), and nad(h) protects the enzyme against this inhibition [phelps, d.c., and hatefi, y. (1984) biochemistry 23, 4475-4480]. the tryptic digest of th treated with [14c]dccd showed a single radioactive peak upon fplc chromatography. this radioactive peak was absent from tryptic digests of th treated with [14c]dccd in the presence of nadh. sequence analysis of the ra ... | 1987 | 3426633 |
| cloning of cdna for darpp-32, a dopamine- and cyclic amp-regulated neuronal phosphoprotein. | a cdna clone for the mrna of bovine darpp-32 (dopamine- and adenosine 3',5'-monophosphate-regulated phosphoprotein, mr = 32,000) was isolated from a modified okayama-berg plasmid library. transformed escherichia coli colonies were screened by in situ colony hybridization with 2 different oligonucleotide probes corresponding to a region unusually rich in glutamate within the protein. three positive clones were isolated and shown to encode darpp-32 by an in situ immunoblot assay of their fusion pr ... | 1988 | 3339428 |
| rapid fluorogenic enumeration of escherichia coli in selected, naturally contaminated high moisture foods. | an assay for the enzyme glucuronidase was used to determine the presence of escherichia coli in selected, naturally contaminated high moisture foods. raw pork sausage, ground turkey, and ground beef were inoculated into tubes containing the substrate 4-methylumbelliferyl beta-d-glucuronide (mug) in lauryl tryptose (lt) medium. after incubation at 35 degrees c for 24 h, the inoculated lt-mug tubes were examined under longwave ultraviolet light for the presence of a fluorogenic glucuronidase end p ... | 1987 | 3325491 |
| growth inhibition of escherichia coli and klebsiella pneumoniae during involution of the bovine mammary gland: relation to secretion composition. | mammary secretions from 12 holstein dairy cows were collected to evaluate growth inhibition of escherichia coli and klebsiella pneumoniae during involution and during physiologic transitions of the mammary gland. mammary secretions obtained during late lactation poorly inhibited growth of e coli and k pneumoniae. however, as involution progressed, mammary secretions increasingly inhibited growth of both coliform mastitis pathogens. greatest inhibition of e coli and k pneumoniae growth was observ ... | 1987 | 3324839 |
| production of recombinant human pancreatic secretory trypsin inhibitor by escherichia coli. | a synthetic gene for human pancreatic secretory trypsin inhibitor (psti) was fused to the coding sequence for the amino-terminal 135 amino acid residues of human interferon-gamma (ifn-gamma) by interposing a methionine codon sequence, and the resulting hybrid gene was efficiently expressed in escherichia coli cells. recombinant human psti (rhu-psti) was separated from the ifn-gamma/psti fused protein by cleavage at the methionine residue with cyanogen bromide. finally, rhu-psti was purified by a ... | 1987 | 3323193 |
| some aspects of dna polymerase functioning. | the group of dna polymerases was studied using some new nucleoside 5'-triphosphate analogs with termination substrate properties. among dna polymerases tested the least specific appeared reverse transcriptases of retroviruses and the most specific were dna polymerases alpha type from high eucaryotes including mammalians. | 1987 | 3320974 |
| alternative procedure for the purification of the heat-stable enterotoxin of enterotoxigenic escherichia coli pathogenic for calves. | a method for purification of the heat-stable enterotoxin (st) of enterotoxigenic escherichia coli (etec) strains (c1444 and b41) pathogenic for calves and some physiochemical properties of the st are described. the method involved ultrafiltration on pm-10 and um-2 diaflo membranes, acetone fractionation, ion-exchange chromatography on ag 1-x2, chromatofocusing and a combination of hydrophobic interaction chromatography on octyl-sepharose cl-4b and gel-permeation on bio-gel p-2. polyacrylamide ge ... | 1987 | 3320075 |
| clinical pharmacokinetics of five oral cephalosporins in calves. | the minimal inhibitory concentrations (mic) of cephalexin, cephradine, cefaclor, cefatrizine and cefadroxil for salmonella species, escherichia coli and pasteurella multocida isolated previously from young calves were determined. the mic90 values for cephalexin, cephradine and cefadroxil ranged between 3.12 micrograms ml-1 and 12.5 micrograms ml-1, whereas those of cefatrizine and cefaclor were 3.12 micrograms ml-1 and 0.78 microgram ml-1, respectively. each drug was administered intravenously a ... | 1987 | 3317582 |
| incidence of the aerobactin iron uptake system among escherichia coli isolates from infections of farm animals. | to assess the importance of aerobactin-mediated iron uptake as a bacterial virulence determinant in animal infections, a total of 576 strains of escherichia coli isolated from cattle, chickens, sheep and pigs were screened by colony hybridization to determine the presence of the aerobactin genetic determinants, and by a bioassay to detect aerobactin secretion in iron-limited conditions. results obtained by the two complementary methods correlated well. the incidence of the aerobactin system was ... | 1987 | 3309175 |
| cloning and sequencing of a carp beta s-crystallin cdna. | the mrnas were extracted from common carp (cyprinus carpio) lenses, purified, reverse transcribed, dc tailed and cloned into escherichia coli with pbr322 as vector. the cloning efficiency was around 1 x 10(7) colonies per micrograms of mrna. a clone (pc20) was found by hybrid-arrested to contain the cdna related to carp crystallins. however, comparison of the derived amino-acid sequence with bovine gamma-ii and beta s-crystallins indicates that this carp crystallin sequence resembles closely the ... | 1987 | 3307924 |
| effect of intramammary devices on the outcome of induced escherichia coli infection of bovine mammary quarters. | eighteen holstein cows, free of intramammary infection, were fitted with smooth (n = 9) or abraded (n = 9) intramammary devices (imd) in 2 diagonally opposed quarters within 4 weeks after calving. the 2 other quarters of each cow were used as controls. three to 6 weeks after imd insertion, depending on when milk somatic cell counts returned to a base-line value of less than 4 x 10(5)/ml, all cows were subjected to bacterial challenge exposure in the front or rear quarters by intracisternal injec ... | 1987 | 3307564 |
| enteric infections caused by non-enterotoxigenic escherichia coli in animals: occurrence and pathogenicity mechanisms. a review. | not all e. coli that cause diarrhoea in farm animals act by elaborating the classical heat-labile or heat-stable enterotoxins. these newly recognised animal enteropathogenic e. coli (epec) attach to and efface the microvilli of the gut epithelium and resemble the well known human epec. in rabbits, only this type of e. coli enteritis is known to be important and a similar disease has been shown to occur in cattle also. there is no doubt that adherence factors are important in the pathogenesis of ... | 1987 | 3307126 |
| changes in blastogenic activity of bovine blood mononuclear cells throughout the nonlactating period. | mononuclear cells were isolated from bovine blood by density gradient centrifugation to determine variation in mitogen-induced mononuclear cell activity throughout the nonlactating period. in a preliminary study, optimum concentrations of concanavalin a, phytohemagglutinin, salmonella typhimurium lipopolysaccharide, and three escherichia coli lipopolysaccharides were determined using six cows as blood donors. concanavalin a, phytohemagglutinin, and e. coli 0111:b4 lipopolysaccharide were selecte ... | 1988 | 3292610 |
| contribution of various host factors to resistance to experimentally induced bacterial endotoxemia in calves. | the reactions of 15 calves to iv administered bacterial lipopolysaccharide was investigated and was correlated with the capacity of 2 host defense mechanisms. the calves had a wide range of changes in clinical response, total wbc count, plasma lactate dehydrogenase (ld) activity, reaction to intradermally inoculated lipid a, and rectal temperature response. the early rectal temperature response of an individual calf was correlated with plasma ld activity, indicating a relationship between cell d ... | 1988 | 3288022 |
| relationship of milk proteins in blood with somatic cell counts in milk of dairy cows. | intramammary leucocytosis was induced by injection of escherichia coli endotoxin via the teat canal in three lactating holstein cows. concentrations of alpha-lactalbumin and casein in blood serum were measured, and somatic cell concentration and yield and composition of milk were determined. endotoxin injection elicited mean increases of 100-fold in somatic cell concentration and 50% in protein concentration, whereas milk yield declined 5-fold and lactose concentration was halved. concentrations ... | 1988 | 3286699 |
| molecular cloning and expression of bovine kappa-casein in escherichia coli. | a cdna library was constructed using poly(a) +rna from bovine mammary gland. this cdna library of 6000 clones was screened employing colony hybridization using 32p-labelled oligonucleotide probes and restriction endonuclease mapping. the cdna from the selected plasmid, pkr76, was sequenced using the dideoxy-chain termination method. the cdna insert of pkr76 carries the full-length sequence, which codes for mature kappa-casein protein. the amino acid sequence deduced from the cdna sequence fits t ... | 1988 | 3286696 |
| rabbit pasteurellosis: induced disease and vaccination. | pasteurellosis was induced in rabbits by conjunctival inoculation with 2 strains of pasteurella multocida. the ld50 of strain p1062 (a bovine isolate) was 10(5.1) colony-forming units and that of strain p1059 (a turkey isolate) was 10(5.5) colony-forming units. pasteurella-free rabbits were vaccinated iv or mucosally with boiled cells of p multocida or a cross-reactive uridine diphosphogalactose epimerase-deficient mutant of escherichia coli j5. in rabbits challenge exposed with p multocida stra ... | 1988 | 3282457 |
| effects of source and washing of erythrocytes on growth of bacterial pathogens from the bovine mammary gland. | effects of source and washing of rbc on quantitative growth and hemolytic zone sizes of common bacterial pathogens of the bovine mammary gland were evaluated. blood samples used to prepare the blood agar media were obtained from 10 adult dairy cows, 10 dairy calves, and 10 sheep. hemolytic zone sizes produced by staphylococcus aureus were significantly (p less than 0.01) larger on blood agar prepared with washed rbc than on blood agar prepared with nonwashed rbc, regardless of rbc source. with t ... | 1988 | 3279872 |
| reduction studies on bacterial recombinant somatomedin c/insulin-like growth factor-1. | n-met-somatomedin c/insulin-like growth factor-1 (rsmc) had been produced in recombinant escherichia coli in monomeric form. the intact rsmc peptide is initially synthesized in e. coli cells in denatured form as inclusion bodies. the rsmc peptide in these inclusion bodies was found in reduced form. isolation of this rsmc peptide was accomplished by separation and dissolution of the inclusion bodies, with dissociation of non-covalently aggregated species. the reduced rsmc was converted to a metas ... | 1988 | 3170686 |
| multiple interactions of lysine-128 of escherichia coli glutamate dehydrogenase revealed by site-directed mutagenesis studies. | a highly conserved lysine at position 128 of escherichia coli glutamate dehydrogenase (gdh) has been altered by site-directed mutagenesis of the gdha gene. chemical modification studies have previously shown the importance of this residue for catalytic activity. we report the properties of mutants in which lysine-128 has been changed to histidine (k128h) or arginine (k128r). both mutants have substantially reduced catalytic centre activities and raised ph optima for activity. k128h also has incr ... | 1988 | 3149742 |
| enzymatic amidation of recombinant (leu27) growth hormone releasing hormone-gly45. | by chemoenzymatic synthesis the gene for a (leu27) analogue of human growth hormone releasing hormone-gly45 [(leu27)ghrh-gly45] was constructed, cloned and expressed in escherichia coli as a fusion protein with beta-galactosidase under the control of the lac promoter and operator. upon induction with isopropyl-d-thio-beta-galactopyranoside the fusion protein accumulated to a yield of 15-20% of the total cellular protein. after cyanogen bromide cleavage of the fusion protein the precursor peptide ... | 1987 | 3148933 |
| nucleotide sequence of chicken myb proto-oncogene promoter region: detection of an evolutionarily conserved element. | the nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by gerondakis and bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). sequences homologous to this element were detected on southern blots of restricted genomic dnas from mouse, man, lizard, frog, ... | 1988 | 3145493 |