Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the chromosomal protein mc1 from the archaebacterium methanosarcina sp. chti 55 induces dna bending and supercoiling. | we have investigated the effect on the dna structure of protein mc1, a basic and small polypeptide (mr 10700) representing the major chromosomal protein in methanosarcinaceae. the ability of protein mc1 to strongly favour cyclization upon polymerization of short dna fragments by t4 dna ligase indicates that protein mc1 mediates dna bending. several negatively supercoiled topoisomers of minicircles were obtained with dna fragments of 203 and 146 bp, their distribution depends upon the amount of p ... | 1991 | 2057361 |
| effect of thyroid hormones on hatching in the tilapia, oreochromis mossambicus. | incubation of fertilized eggs of oreochromis mossambicus in media containing varying doses of t3, t4, and phenylthiocarbamide (ptc) indicated that the thyroid hormones delayed hatching, while the antithyroid drug accelerated it. denuded eggs were also incubated in the above media and the media were assayed at various time intervals for hatching enzyme. the thyroid hormones delayed hatching enzyme release, while ptc enhanced it. the possible role of thyroid hormones in the control of the hatching ... | 1991 | 2055445 |
| cortisol effects on plasma electrolytes and thyroid hormones during smoltification in coho salmon oncorhynchus kisutch. | plasma concentrations of na+, k+, triiodothyronine (t3), and thyroxine (t4) and muscle water content were measured in yearling coho salmon. oncorhynchus kisutch, after injection of cortisol in april, may, and june in fresh water (fw) and during acclimation to seawater (sw). cortisol (17-21 micrograms/g), injected intraperitoneally in a melted cocoa butter suspension, suppressed the rise of plasma na+ during sw acclimation in april but not in may or june. muscle water content increased during sw ... | 1991 | 2055437 |
| thyroid hormone increases muscle/fat glucose transporter gene expression in rat skeletal muscle. | the enhancement of metabolic rate by thyroid hormone in target tissues is accompanied by increased glucose utilization, which in skeletal muscle and many other tissues is regulated at the level of membrane transport. to elucidate the role of thyroid hormone as a regulator of glucose transport in skeletal muscle, we have examined its effects on the expression of the muscle/fat (insulin-regulatable) glucose transporter, a distinct glucose transporter isotype whose expression is limited to insulin- ... | 1991 | 2055200 |
| maternal hypothyroidism during the first half of gestation compromises normal catabolic adaptations of late gestation in the rat. | female rats were mated and thyroidectomized on the same day. some animals were kept without treatment and killed on day 12 or 21 of gestation (t). others were subsequently treated daily with 1.8 micrograms l-t4/100 g bw for either the first 12 days and then not treated from that time until day 21 [t+t4(i+0)] or else not treated for the first 12 days and then treated from days 12-21 [t+t4(0+ii)]. a final group received treatment during the entire 21-day study [t+t4(i+ii)] and was used as the cont ... | 1991 | 2055183 |
| role of the thyroid gland in seasonal reproduction. ii. thyroxine allows a season-specific suppression of gonadotropin secretion in sheep. | a series of four experiments was conducted to examine the previously described phenomenon that the thyroid gland is required for the breeding season to end in female sheep. exp 1 tested the hypothesis that the thyroid is required because of its secretion of t4. ewes thyroidectomized (thx) in the anestrous season either received t4 replacement or no further treatment; seasonal reproductive shifts were compared to those of thyroid-intact controls. all ewes were housed in conditions in which the en ... | 1991 | 2055181 |
| relationship between maternal thyroxine levels during pregnancy and memory function in childhood. | two investigations are reported into the memory functions of a group of subjects for whom information on maternal thyroxine (t4) levels during pregnancy is available. the first study made when the subjects were aged 14-15 years examined forward and backward memory span. no relationship between maternal t4 levels and capacity of the short-term store, measured as length of forward memory span, was found. backward memory span, which involves performing a mental operation on the material held in sto ... | 1991 | 2055175 |
| immune dysfunction in children after corrective surgery for congenital heart disease. | to study the effect of open- and closed-heart surgery on the immune status of infants and children. | 1991 | 2055075 |
| the effects of carbamazepine on resting metabolic rate and thyroid function in depressed patients. | resting metabolic rate and thyroid hormone levels were studied in 11 patients with affective disorders before and during treatment with carbamazepine (cbz). cbz has been previously reported to reduce thyroid hormones, but the metabolic consequence of this effect has not been explored. during cbz treatment, thyroid hormones decreased significantly (t4, 7.53 versus 5.74 micrograms/dl, p less than 0.001), whereas the resting metabolic rate (rmr) did not (31.6 versus 30.7 kcal/m2/hr). baseline rmrs ... | 1991 | 2054452 |
| cold agglutinins in haemophiliac boys infected with hiv. | eleven haemophiliac boys infected with hiv were screened for irregular red cell antibodies and were compared with nine haemophiliac boys who did not have antibodies to hiv. seven (64%) of the children who had antibodies to hiv also had cold agglutinins, mostly of anti-i specificity, compared with one (11%) of those who did not have antibodies to hiv. the children with antibodies to hiv and cold agglutinins had a significantly increased mean igm concentration. the presence of cold agglutinins was ... | 1991 | 2053801 |
| stimulating effect of forskolin on thyroxine secretion. influence of calcium. | the aim of this study was to determine, with a rat thyroid fragment perifusion system, the short-term (20 min) effect of forskolin (fk), an adenylate cyclase activator, on t4 secretion, the effect of forskolin on t4 secretion stimulated by theophylline and tsh, and the role played by calcium in the forskolin effect. a dose-dependent effect on t4 secretion was obtained with forskolin, from 10(-8) to 10(-4) mol/l, with a maximal effect between 10(-6) and 10(-5) mol/l. the effect of forskolin was n ... | 1991 | 2052424 |
| [t4 advanced lung cancer: results of surgical treatment and indications of surgical resection]. | a total of 1,289 patients with primary lung cancer were surgically treated at our hospital from january 1953 to december 1985. surgical treatment for t4 lung cancer was studied in 93 patients who had pulmonary resections. the relationships between histologic type, stage, method of resection, curability, nodal involvement, pleural involvement, site of invasion, pleural metastasis, pleural effusion, combination therapy, and the survival rate were analyzed. the survival rate of 93 patients with t4 ... | 1991 | 2051675 |
| acute l-thyroxine overdose; therapy with sodium ipodate: evaluation of clinical and physiologic parameters. | two children with acute l-thyroxine overdose were treated with sodium ipodate, an oral cholecystographic agent. initial thyroxine (t4) levels were elevated to 98.5 mcg/dl and 134.1 mcg/dl, with associated triiodothyroxine (t3) levels of 354 ng/dl and 402 ng/dl. t3 levels increased to a maximum of 662 ng/dl and 468 ng/dl. with administration of sodium ipodate, the t3 decreased with a simultaneous increase of rt3 level. sodium ipodate effect lasted 72 hours. no toxic effect was noted. interestingl ... | 1991 | 2050969 |
| primary radiotherapy versus surgery and radiotherapy in the management of postmenopausal locally advanced breast cancer. | from 1978 through 1984, 103 postmenopausal patients with locally advanced breast cancer (t4 n1-2-3 mo) were treated by definitive radiotherapy (xrt, 35 patients) or mastectomy and postoperative radiotherapy (rm + xrt, 68 patients). a control group of 35 of the 68 rm + xrt patients was selected by matching several prognostic variables with the xrt group. patients were followed for 4 to 10 years. at 5 years the probabilities of overall survival and relapse-free survival were 49.9% and 33.2% for xr ... | 1991 | 2048226 |
| effects of selection on plasma thyroxine concentrations in japanese quail under thiouracil and protein stress. | three experiments were conducted to measure changes in plasma thyroxine (t4) concentrations occurring in male and female japanese quail in response to treatment with dietary thiouracil (tu) and different cp levels prior to sexual maturity and to determine the influence of selection for growth under tu and protein stress on this response. selected and unselected lines of quail were fed diets containing .2% tu or two levels of cp (20 or 28%) or both from 0 to 4 wk of age. body weight and plasma t4 ... | 1991 | 2047354 |
| evidence suggesting that the sympathetic nervous system mediates thyroidal depression in turpentine-induced nonthyroidal illness syndrome. | acute superior cervical ganglionectomy (scgx) induces in the rat a supraliminal release of neurotransmitter in the innervated tissues (i.e., thyroid gland). this temporary adrenergic hyperactivity is correlated with a significant depression of the thyroid economy resembling the nonthyroidal illness (nti) syndrome in the rat, and suggest that the sympathetic nervous system may mediate thyroidal changes in nti. in order to gain further insight into the thyroidal depression in the nti syndrome, we ... | 1991 | 2046869 |
| effect of propylthiouracil treatment during prenatal and early postnatal development on the neocortex of rat pups. | the effects of a pre- and postnatal hypothyroid environment on the development of the neocortex have been determined. rats were treated with propylthiouracil (ptu) with or without thyroid hormone supplementation during pregnancy and throughout the early development of the pups. the cavalieri method was used to provide an estimate of the volume of the neocortex, and a combination of the cavalieri method and the 'disector' particle-counting method provided an unbiased estimate of the numbers of gl ... | 1991 | 2046865 |
| muscarinic modulation of the vasodilatory effects of vasoactive intestinal peptide at the rat thyroid gland. | in the thyroid gland, vasoactive intestinal peptide (vip) and acetylcholine (ach) are found in nerve fibers associated with secretory cells and blood vessels. we have, therefore, initiated studies to explore the actions of and interactions between cholinergic agents and vip in the regulation of thyroid vascular conductance (vc). thyroid and other organ blood flows were measured using radiolabelled (141ce) microspheres injected directly into the left cardiac ventricle of anesthetized male rats. t ... | 1991 | 2046862 |
| gene 32 transcription and mrna processing in t4-related bacteriophages. | we have analysed transcription and mrna processing for the gene 32 region of five phages related to t4. two different organizations of gene 32 proximal promoters were found. in t4 and m1, middle- and late-mode promoters are separated by 50 nucleotides and located within an upstream open reading frame. in t2, k3, ac3, and ox2, the 626bp t4 sequence that includes these promoters is replaced by a 59bp sequence containing overlapping middle and late promoters. the rnase e-dependent processing of the ... | 1991 | 2046553 |
| pyrimidine dimer induction and repair in cultured human skin keratinocytes or melanocytes after irradiation with monochromatic ultraviolet radiation. | we compared the susceptibilities of cultured melanocytes and keratinocytes to dimer induction in dna by monochromatic ultraviolet (uv) radiation. keratinocytes as well as melanocytes were derived from human foreskin, grown as a monolayer in petri dishes, covered with phosphate-buffered saline containing 0.1% glucose, and irradiated. uv irradiation was carried out at 254, 297, and 302 nm as well as with a light source emitting predominantly 312 nm. the induction of pyrmidine dimers was assessed b ... | 1991 | 2045680 |
| pretreatment with betamethasone of patients with graves' disease given radioiodine therapy: thyroid autoantibody responses and outcome of therapy. | the effects of betamethasone on thyroid autoantibody responses and outcome of radioiodine therapy were determined over a period of 1 yr in a prospective randomized study of 40 patients with graves' disease. twenty patients were given placebo tablets, and 20 patients were treated with beta-methasone from 3 weeks before until 4 weeks after 131i therapy. at the time of inclusion in the study, the mean serum concentrations of tsh receptor antibodies, thyroid peroxidase antibodies, and thyroglobulin ... | 1991 | 2045463 |
| episodic secretion of thyroxine (t4) and triiodothyronine (t3) from porcine thyroid fragments in vitro. | 1991 | 2045066 | |
| thyroid function in sojourners and acclimatised low landers at high altitude in man. | the circulatory levels of t4, t3, rt3, tsh as well as tsh response to trh, thyroid hormone binding proteins and t3 concentration of erythrocytes were studied in (i) healthy euthyroid sea level residents (slr) at sea level, (ii) during three weeks of stay of slr at an altitude of 3500 m (sojourners, sj), (iii) slr staying at high altitude (ha) for 3 months to 10 years (acclimatised low landers. all), (iv) high altitude natives (han) and (v) euthyroid men during intermittent exposure to simulated ... | 1991 | 2045063 |
| the phage t4 nrdb intron: a deletion mutant of a version found in the wild. | bacteriophage t4 possesses three self-splicing group i introns. two of the three introns are mobile elements; the third, in the gene encoding a subunit of the phage nucleotide reductase (nrdb), is not mobile. because intron mobility offers a reasonable explanation for the paradoxical occurrence of large intervening sequences in a space-efficient eubacterial phage, it is puzzling that the nrdb intron is not mobile like its compatriots. we have discovered a larger nrdb intron in a closely related ... | 1991 | 2044951 |
| interactions of halogenated industrial chemicals with transthyretin and effects on thyroid hormone levels in vivo. | previous results in experimental systems have suggested that hydroxylated pcbs may decrease thyroid hormone levels through associative interaction with transthyretin. in the present paper it was investigated whether this property was also shared by various industrial chemicals, mainly pesticides. in total, 65 compounds from 12 chemical groups were analyzed for direct interference with the t4 binding site of transthyretin using a competitive binding assay. sixty per cent of the compounds were com ... | 1991 | 2043046 |
| changes in human plasma catecholamine concentrations during epidural anesthesia depend on the level of block. | to test the hypothesis that increasing levels of epidural analgesia will produce progressive decreases in circulating catecholamines, we sequentially produced three levels of analgesia, t8, t4, and c8, to pin prick in young, healthy volunteers. three percent chloroprocaine (plain) was used as the local anesthetic. the epidural analgesia was allowed to dissipate following the t8 and t4 levels of block. after the c8 level the block was reinforced to study the effect of a "top-up" dose. blood sampl ... | 1991 | 2042758 |
| relation of the pathogenesis of human immunodeficiency virus infection to various strategies for its control. | this paper reviews functionally important insights into the pathogenesis of human immunodeficiency virus (hiv) infection. the major sequela of this infection is early and progressive involvement of the immune system, with widespread immune dysfunction. this pathogenetic feature has a major impact on strategies for control of the infection. the immunosuppression caused by the virus leads to higher levels of viral replication and enhanced potential for development or selection of variant viruses, ... | 1991 | 2041962 |
| total body hyperthermia in the treatment of kaposi's sarcoma in an hiv positive patient. | the effect of prolonged exposure to heat on the hiv has had limited exploration. this is the report of a 33-year-old white man with positive tests for hiv and multiple lesions of kaposi's sarcoma. the patient was exposed to total body hyperthermia of 42 degrees c for two hours. three months following hyperthermia the patient feels improved. the lesions of kaposi's sarcoma have markedly regressed, and the t4 lymphocyte count has risen from 5 per cc to 330 per cc. hiv cultures (blood) remain negat ... | 1991 | 2041382 |
| evidence of hypothalamic-pituitary thyroid abnormalities in children with end-stage renal disease. | patients with end-stage renal disease may have abnormalities of growth and of gonadal and thyroid hormones, so we attempted to determine the mechanisms that may be involved in the altered thyroid function. we evaluated serum thyroid hormone levels, their changes immediately after hemodialysis, the serum thyrotropin (thyroid-stimulating hormone (tsh) response to thyrotropin releasing hormone, and the circadian pattern of serum tsh in nine children with end-stage renal disease who were between 7 1 ... | 1991 | 2040922 |
| isolation and characterization of extragenic suppressor mutants of the tola-876 periplasmic-leaky allele in escherichia coli k-12. | tola mutants of escherichia coli k-12 release periplasmic proteins into the extracellular medium; they are sensitive to growth inhibitors such as cholic acid and tolerant to group a colicins and filamentous bacteriophage. suppressor mutants of the tola-876 allele were isolated by selecting for cholic acid resistant clones that did not release periplasmic ribonuclease i. one class of tola suppressor strains carried mutations in the staa gene (for suppressor of tola) located a 41 min. tola-876 sta ... | 1991 | 2040438 |
| [studies on thyroid hormone autoantibody (thaa) in 2 cases of graves' disease with spuriously high free thyroxine values]. | two patients with graves' disease treated with methimazole (mmi) showed a discrepancy between serum free t4 (ft4) values and other hormone values (especially total t4) which was due to the presence of potent binding activity to labelled t4 analogue (125i-at4) in their serum. this activity was demonstrated to be in immunoglobulin g (igg) with kappa light chain isotype in both patients. the binding of 125i-at4 to their serum was inhibited by unlabelled t4 in a dose-dependent manner. autoantibodies ... | 1991 | 2040367 |
| a new immunoradiometric assay (irma) system for thyroglobulin measurement in the follow-up of thyroid cancer patients. | a new commercially available kit for thyroglobulin (tg) measurement [immunoradiometric assay (irma) system based on monoclonal antibodies] was used in 479 patients with thyroid carcinoma. the effective working range was 1 ng/ml, and results were strongly correlated with our homemade radioimmunoassay (ria). this irma method is less susceptible to interferences of auto-antibodies than our ria. during thyroxine (t4) treatment, the tg level was undetectable in 98% of patients after total thyroid abl ... | 1991 | 2040337 |
| human immunodeficiency virus infection in pregnancy. | many aspects of hiv infection in pregnancy remain unclear. subsets at increased risk for perinatal transmission, adverse pregnancy outcome, and development of symptomatic hiv infection need to be identified. for instance, relative risks may be quite different in asymptomatic hiv infected patients with t4 lymphocyte counts greater than 200 cells per cubic millimeter compared to those with either symptoms of hiv infection or t4 cell counts less than 200 cells per cubic millimeter. at present, anti ... | 1991 | 2040117 |
| oxygenation of human tumors: evaluation of tissue oxygen distribution in breast cancers by computerized o2 tension measurements. | direct oxygen partial pressure (po2) readings in breast cancers, in fibrocystic disease, and in the normal breast have been obtained using a novel technique which allows for the systematic evaluation of the oxygenation status as a function of pathological staging and histological grading. measurements were performed in awake pre- and postmenopausal patients with well-defined arterial blood gas status. the measuring procedure encompasses a computerized electrode movement in the tissue which avoid ... | 1991 | 2040005 |
| [ovariopexy and the treatment of hodgkin's disease]. | between 1979 and 1989 a total of 113 women underwent treatment for hodgkin's disease at the department of radiation oncology of the university of erlangen-nürnberg. only 17 female patients of child bearing age received total lymphoid irradiation including pelvic and inguinal nodes. 15/17 patients underwent prophylactic bilateral oophoropexy during staging laparotomy: ten had lateral, five had midline ovarian transposition. reproductive and ovarian function was investigated in 13 patients--all in ... | 1991 | 2038709 |
| [evaluation of surgical indication for the small lesion of advanced lung cancer]. | this report analyzes the operative indication for the small lesion of advanced lung cancer. the subjects consisted of 25 patients with t1n2 lung cancer, one t1n3, four t1m1 and five small lung cancer lesion with dissemination, which was regarded as the small lesion of advanced lung cancer. the cumulative 5-year survival rate after operation for 25 patients with t1n2 lesion was 30.6%. of 25 patients, 18 were selected patients who underwent a curative operation with a 5-year survival of 37.0%. in ... | 1991 | 2038145 |
| endocrine abnormalities in severe traumatic brain injury--a cue to prognosis in severe craniocerebral trauma? | patients with severe craniocerebral trauma (scct) display metabolic and endocrine changes. the question is raised whether hormonal patterns give cues to the prognosis of outcome or not. in 21 patients the function of the adrenocortical, gonadal, thyroid and human growth hormone (hgh)-insulin system was assessed. lh, fsh, tsh, prolactin and hgh were stimulated. 3 groups of patients were formed. group i: patients in acute phase with a glasgow coma score (gcs) more than 6 (group ia) and less than 6 ... | 1991 | 2037721 |
| myxoedema coma: response of thyroid hormones with oral and intravenous high-dose l-thyroxine treatment. | myxoedema coma is a medical emergency with high mortality. in this study, clinical response and plasma variations of thyroid hormones were analysed in 7 patients, 6 presenting with myxoedema coma and one with myxoedema ileus. these patients were treated with intravenous or oral l-thyroxine (l-t4). 1000 mu l-t4 iv were administered in two patients. within 3 h, plasma t4 and triiodothyronine (t3) reached a peak upper normal range, then diminished slowly during 5-9 days. the 5 remaining patients we ... | 1991 | 2037720 |
| selective metal binding to cys-78 within endonuclease v causes an inhibition of catalytic activities without altering nontarget and target dna binding. | t4 endonuclease v is a pyrimidine dimer-specific dna repair enzyme which has been previously shown not to require metal ions for either of its two catalytic activities or its dna binding function by virtue of its ability to function in the presence of metal-chelating agents. however, we have investigated whether the single cysteine within the enzyme was able to bind metal salts and influence the various activities of this repair enzyme. a series of metals (hg2+, ag+, cu+) were shown to inactivat ... | 1991 | 2037608 |
| effect of hypothyroidism and thyroid hormone replacement on the level of protein kinase c and protein kinase a in rat liver. | we investigated the influence of the thyroid hormone status on the levels of protein kinases c (pkc) and a (pka) in the soluble fraction of rat liver. the immunodetectable pkc level in hypothyroid liver was elevated 7.7-fold, whereas the phorbol-ester binding capacity and the immunodetectable alpha-pkc level were increased 2.4- and 2.6-fold, respectively. conversely, in hypothyroid livers the abundance of the regulatory type i and the catalytic subunits of pka were lowered to 42% of the euthyroi ... | 1991 | 2037057 |
| iodothyronine 5'-deiodinase activity in cultured rat myocardial cells: characteristics and effects of triiodothyronine and angiotensin ii. | using a primary culture of neonatal rat heart, we investigated the properties of iodothyronine 5'-deiodinating activity in the heart. 125i- release from [125i] reverse t3 incubated with cell homogenates depended on the concentrations of protein and dithiothreitol, ph, incubation time, and temperature of the incubation mixture. the activity was almost completely inhibited by 10(-3)-10(-5) m propylthiouracil and had the higher affinity for reverse t3 than t4. these findings indicate that type i io ... | 1991 | 2036980 |
| effect of growth hormone therapy on thyroid status of girls with turner's syndrome. | the effect of recombinant human growth hormone (rhgh) administration on thyroid function was studied in 14 girls with turner's syndrome for 18 months. all patients were euthyroid and had no documented gh deficiency. treatment with rhgh, administered as daily subcutaneous injections in a dose of 0.15 iu/kg/day, led to a decrease of serum t4 levels from 124 +/- 4 (mean +/- sem) to 106 +/- 4 nmol/l (p less than 0.05) after 6 months of therapy. thereafter serum t4 levels returned progressively to ba ... | 1991 | 2036729 |
| [nasal cavity and paranasal sinus malignancies in the ent clinic of charité since 1959]. | a retrospective analysis of 235 cases suffering from nasal and/or paranasal sinus malignant tumours who were admitted to the charité ent-clinic between 1959 and 1989, is presented. 94 of the patients were women and 141 men, the mean age being 63.8 years (women) and 61.8 years (men), respectively. the most common histomorphological diagnosis was carcinoma (66%), followed by lymphoma/sarcoma (17%). 35% of all tumours originated in the nasal cavity, 38% in the maxillary sinus, 22% in the ethmoidal ... | 1991 | 2036148 |
| effect of oral administration of prednisolone on thyroid function in dogs. | to determine the effect of oral administration of prednisolone on thyroid function, 12 healthy beagles were given 1.1 mg of prednisolone/kg of body weight every 12 hours for 22 days after 8 days of diagnostic testing of the dogs before treatment with prednisolone. thyroid-stimulating hormone (tsh) and thyrotropin-releasing hormone (trh) response tests were performed before treatment (days 1 and 8 of the study) and during treatment (days 21 and 28 of the study). blood samples were collected daily ... | 1991 | 2035915 |
| comparison of hypobaric, hyperbaric, and isobaric solutions of bupivacaine during continuous spinal anesthesia. | this study was designed to compare the anesthetic properties of hypobaric bupivacaine with those of isobaric and hyperbaric solutions when administered in the supine position in an elderly population undergoing hip surgery using continuous spinal anesthesia. plain bupivacaine (0.5%) was mixed with equal volumes of 10% dextrose (hyperbaric), 0.9% nacl (isobaric), or distilled water (hypobaric) to obtain 0.25% solutions. in a double-blind fashion, all patients received 3 ml (7.5 mg) of their parti ... | 1991 | 2035860 |
| measurement of thyroid hormone in the rat thyroid gland by radioimmunoassay. | the present paper describes (i) a hydrolysis technique with pronase and leucine aminopeptidase using one rat thyroid gland, resulting in maximum release of thyroid hormones and minimum deiodination, and (ii) a simple and rapid procedure for thyroid hormone radioimmunoassays in thyroid hydrolysates using commercial kits intended for serum thyroid hormone determinations. the procedure is used to determine t4, t3, and rt3 concentrations and hormonal molar ratios in a thyroid gland from a male wista ... | 1991 | 2035829 |
| spine deformity index (sdi) versus other objective procedures of vertebral fracture identification in patients with osteoporosis: a comparative study. | radiologic identification of vertebral fractures is most important in the diagnosis and monitoring of patients with spinal osteoporosis. different methods, using vertebral height measurements for fracture identification, have therefore been developed. we compared four methods for fracture identification in spinal x-rays of 62 female patients with primary osteoporosis. the methods of hedlund and gallagher, melton et al., and davies et al. are based on the ratio of heights within one vertebra or o ... | 1991 | 2035349 |
| classification of vertebral fractures. | although it is a cardinal feature of involutional osteoporosis, there is often disagreement on what constitutes a vertebral fracture. we measured vertebrae t4-l5 in 52 healthy women to develop a normal range (mean +/- 3 sd) for vertebral shape and used these data to assess the prevalence of vertebral fractures. we classified vertebral fractures by type of deformity (wedge, biconcavity, or compression) and further by the degree of deformity (grades 1 and 2). in 195 postmenopausal women who were a ... | 1991 | 2035348 |
| [risk factors for local recurrence of rectal carcinoma]. | the impact of distal resection margins and the mode of operation on pelvic recurrence rate was assessed in 331 cases following abdomino-perineal resection (apr; n = 134), and anterior resection (ar; n = 197) for rectal cancer. local recurrence was observed in 55 cases (16.6%) after a median interval of 16 months. only 20 of 212 patients (9.4%) without positive lymph nodes developed a local recurrence, but 35 of 119 (29.4%) with nodal involvement. the recurrence rate was 33% (10/30) in cases with ... | 1991 | 2035278 |
| full-length cd4 electroinserted in the erythrocyte membrane as a long-lived inhibitor of infection by human immunodeficiency virus. | recombinant full-length cd4 expressed in spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (rbc) membranes. of the inserted cd4, 70% was "correctly" oriented as shown by fluorescence quenching experiments with fluorescein-labeled cd4. the inserted cd4 displayed the same epitopes as the naturally occurring cd4 in human t4 cells. double-labeling experiments (125i-cd4 and 51cr-rbc) showed that the half-life of cd4 electroinserted in rbc membrane in rabbits ... | 1991 | 2034680 |
| analysis of mutagenesis induced by a thermolabile t4 phage deoxycytidylate hydroxymethylase suggests localized deoxyribonucleotide pool imbalance. | to understand the molecular basis of mutation stimulated by deoxyribonucleotide pool imbalance, we studied a temperature-sensitive t4 phage gene 42 mutant (lb3), which specifies a thermolabile deoxycytidylate hydroxymethylase. analysis of rii mutations, revertible to wild type along either gc-to-at or at-to-gc transition pathways, showed 8- to 80-fold stimulation of gc-to-at mutations at a semi-permissive temperature (34 degrees c). one such marker, rii sn103, which showed the highest stimulatio ... | 1991 | 2034218 |
| decreased thyroid hormone-stimulated oxygen consumption and glucose uptake in mononuclear blood cells from patients with autosomal dominant osteopetrosis type i. | nine patients, from four different families, with autosomal dominant osteopetrosis were investigated. they all had roentgenological type i disease, characterized by universal, symmetrical osteosclerosis and enlarged thickness of the cranial vault. all patients appeared clinically euthyroid. thyroxine (t4) and tri-iodothyronine (t3) induced oxygen consumption and glucose uptake were studied in vitro in mononuclear blood cells from patients and control persons. unstimulated oxygen consumption from ... | 1991 | 2034033 |
| qualitative evaluation of in-house immunoassays of t3, t4 and tsh based on bulk reagents. | increase in the cost of ria kit assays has led to our attempts to seek cheaper alternates. assays based on bulk reagents (supplied free of cost by inmol in collaboration with iaea) were started in 1988. statistical and quality control data on 50,51 and 52 assay batches of t3, t4 and tsh respectively has been collected from the beginning. cumulative assay parameters show that t3 and t4 assays are almost equally precise. tsh assay is most imprecise in the group especially at low concentration leve ... | 1991 | 2033784 |
| aids: the disease, the demographics, the devastation. | the etiology and pathophysiology of aids are reviewed: hiv-1 infection and changes in t4 lymphocytes; secondary infections, such as pneumocystis carinii pneumonia and tuberculosis; and tumors, such as kaposi's sarcoma. the cdc has classified hiv infection into four groups based on clinical manifestations and immune system changes. male homosexuals and bisexuals are most at risk for hiv infection. the incidence of infection among infants and heterosexuals will increase. public health education pr ... | 1991 | 2033483 |
| simultaneous identification of eight leucocyte subsets of human peripheral blood using three-colour immunofluorescence flow cytometric analysis. | the full analytical potential of flow cytometry has been exploited in order to identify a maximum of human peripheral blood leucocyte subsets in a single tube. for this purpose a mixture composed of six different monoclonal antibodies directly coupled to fitc or phycoerythrin or indirectly linked to the duochrome reagent via the biotin/streptavidin system was used. this combination of monoclonal antibodies and fluorochromes offered the possibility of simultaneously determining eight leucocyte su ... | 1991 | 2033277 |
| [significance of assaying serum bone gla protein level in patients with hyperthyroidism]. | the serum bgp level was assayed in patients with hyperthyroidism (untreated and remittent cases). the mean serum bgp concentration was 8.7 +/- 2.5 ng/ml in 54 patients with untreated hyperthyroidism, which was significantly higher than normal range (4.8 +/- 1.3 ng/ml p less than 0.01). serum bgp had a significant positive correlation with the concentration of t3 and t4, while serum akp had no correlation with circulating thyroid hormone levels. in the patients with hyperthyroidism, serum t3 and ... | 1991 | 2032496 |
| carcinoma of the nasal cavity and paranasal sinuses: an analysis of radical radiotherapy. | a retrospective analysis was conducted of 152 patients with carcinoma of the nasal cavity and paranasal sinuses who were treated with radical radiotherapy between 1975-1984. stages at presentation were t1 11%, t2 18%, t3 27%, and t4 44%. the median follow-up was 61 months. the overall corrected 5-year survival was 47% and by t stage, t1 86%, t2 82%, t3 49%, t4 25%. local control was achieved in 45% of cases following radiotherapy. salvage surgery was attempted in 20 patients where radiotherapy f ... | 1991 | 2031888 |
| inhibition of heterologous strains of hiv by antisense rna. | antisense rna can inhibit the expression of messenger rnas (mrnas) to which they are complementary by a variety of mechanisms and might provide the basis for antiviral therapies of high selectivity. in a previous study of six retrovirally expressed antisense rnas targeted to hiv-1iiib, we found that two significantly reduced hiv-1iiib replication. here we test the degree to which this inhibitory effect tolerates the natural variation found in the nucleotide sequence of different strains of hiv-1 ... | 1991 | 2031688 |
| age, sex, and serum thyrotropin concentrations in primary hypothyroidism. | we studied the relationship between age, sex, serum thyroxine concentrations, and serum thyrotropin concentrations in 202 patients with primary hypothyroidism whose ages ranged from 10 to 89 years. the results from two groups of patients were analysed, both combined and separately, by multiple linear regression analysis of the factors age, sex, group, and serum free t4 index or serum t4 concentration, to predict serum tsh. the serum free t4 index or t4 values and age were negatively correlated w ... | 1991 | 2031434 |
| effect of endosulfan on thyroid physiology in the freshwater catfish, clarias batrachus. | effects of endosulfan on thyroid physiology have been studied in the female freshwater catfish. clarias batrachus during the pre-spawning and spawning phases of its annual reproductive cycle. effects of endosulfan varied with the length (96 h and 16 days) of exposure, reproductive status of the fish and organ. the 96-h endosulfan exposure significantly increased the level of thyroxine (t4) in serum and pharyngeal thyroid follicles concurrent with induction of peroxidase activity. however, triiod ... | 1991 | 2031252 |
| superiority of thyrotropin to thyroxine as a tool in the screening for congenital hypothyroidism by the filter paper spot technique. | the effects of storage on the assessment of thyroxine (t4) and thyrotropin (tsh) were evaluated in blood spots collected on filter paper according to the methods commonly used in screening for congenital hypothyroidism. comparisons were made with serum values obtained simultaneously in the same subjects. in samples stored at room temperature a clear-cut decrease in t4 was observed after 24 hours while tsh was stable for at least 15 days. spot samples collected in an area of severe endemic goiter ... | 1991 | 2029777 |
| detection and identification of benzo[a]pyrene-dna adducts by [35s]phosphorothioate labeling and hplc. | two generally applicable [35s]phosphorothioate postlabeling procedures for the hplc analysis of polycyclic aromatic hydrocarbon (pah)-dna adducts have been developed based upon [32p]phosphate postlabeling assays described by gupta and randerath et al. in one procedure, benzo[a]pyrene (b[a]p)-modified dna was digested to nucleoside 3'-phosphates by micrococcal nuclease and spleen phosphodiesterase and the adducted nucleotides were extracted with 1-butanol. the adducted nucleoside-3'-phosphates we ... | 1991 | 2029754 |
| cisplatin, vindesine, and 96-h continuous infusion of 5-fluorouracil as initial chemotherapy for locally advanced head and neck carcinoma. | the combination of cisplatin (80 mg/m2, i.v., day 1), 96-h infusion of 5-fluorouracil (5-fu) (30 mg/kg/24 h) and vindesine (3 mg/m2 days 1 and 8) was evaluated in 31 patients with histologically proven epidermoid carcinoma of the head and neck. all patients were nonpretreated and had measurable lesions. three (10%) of the 29 evaluable patients achieved a complete response (cr) and 19 (66%) had partial response (pr) with an overall response rate (cr + pr) of 76%. the major toxicity was leukopenia ... | 1991 | 2028924 |
| the molecular mechanism of inhibition of alpha-type dna polymerases by n2-(butylphenyl)dgtp and 2-(butylanilino)datp: variation in susceptibility to polymerization. | calf thymus dna polymerase alpha (pol alpha) and bacteriophage t4 dna polymerase (pol t4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of n2-(p-n-butylphenyl)dgtp (bupdgtp) and 2-(p-n-butyl-anilino)datp (buadatp) on the bupdntp-susceptible alpha polymerase family. kinetic analysis of inhibition of pol alpha with mixtures of complementary and noncomplementary template:primers indicated that both nucleotides induced the formation of a polymerase: inhi ... | 1991 | 2027770 |
| the prognostic significance of thyroid function in mania. | free thyroxine index (ft4i) and thyroxine (t4) levels were measured in 31 manic patients shortly after admission to a psychiatric hospital. over one-third had elevated thyroid hormone levels, and this was largely due to increases in ft4i. increased ft4i levels were associated with greater sleep disturbance and with being male, and were negatively associated with having had hospital admissions in the past six months. more interestingly, however, low ft4i levels prospectively predicted more hospit ... | 1991 | 2027093 |
| reinke's oedema and thyroid function. | reinke's oedema (ro) of the vocal folds is a condition of unknown aetiology. cigarette smoking and vocal abuse may contribute to its development. hypothyroidism has been described as an aetiological factor but, to date, no controlled study has been published confirming this association. this prospective, controlled study compared thyroid function in 61 consecutive ro patients with an age and sex matched control group (n = 65) without laryngeal disease. thyroid function was assessed by measuring ... | 1991 | 2026943 |
| measurement of bone collagen degradation in hyperthyroidism and during thyroxine replacement therapy using pyridinium cross-links as specific urinary markers. | urinary excretion of the bone collagen derived pyridinium cross-links pyridinoline (pyd) and deoxypyridinoline (dpd) was measured in 19 patients (4 m:15 f) with untreated thyrotoxicosis, and 20 pre-, and 20 postmenopausal women taking t4 100-200 micrograms daily for autoimmune hypothyroidism. both pyd and dpd excretion (nanomoles per mmol creatinine) was elevated in the thyrotoxic patients compared to 287 controls; median 131 vs. 26 and 37.5 vs. 7.2, respectively, p less than 0.0001. in premenop ... | 1991 | 2026741 |
| a therapeutic dilemma: suppressive doses of thyroxine significantly reduce bone mineral measurements in both premenopausal and postmenopausal women with thyroid carcinoma. | we measured lumbar spine, femoral neck, and forearm bone mineral (bmd) in 24 women (14 premenopausal and 10 postmenopausal) who had been treated with total thyroidectomy and 131 iodine ablation therapy for nonanaplastic thyroid carcinoma and 24 case controls. at the time of the study, all patients were free of cancer (negative 131 iodine whole body scan and serum thyroglobulin levels less than 0.3 micrograms/l) and all were receiving doses of t4 sufficiently high to prevent a rise in a serum thy ... | 1991 | 2026740 |
| superiority of the use of a pre-incubated complex of primary and second antibody in the radioimmunoassay of thyroid hormones. | we report on the superiority of a radioimmunoassay (ria) system wherein the second antibody (ab2) is incorporated as a pre-incubated complex with the primary antibody (ab1) for the assay of haptens like triiodothyronine (t3) and thyroxine (t4). separation of the antibody bound and free antigen (ag) was accomplished by 8% polyethylene glycol (peg) (final concentration) following a single incubation of less than 1 h. the other advantages of this system are a 15-fold reduction in the quantity of ab ... | 1991 | 2026494 |
| interactive effects of thyroxine and experimental location on running endurance, tissue masses, and enzyme activities in captive versus field-active lizards (sceloporus undulatus). | this study investigates the effects of exogenous thyroxine (t4) on running endurance, tissue masses, and the activities of citrate synthase (cs), pyruvate kinase (pk), cytosolic alpha-glycerophosphate dehydrogenase (alpha-gpdh), and beta-hydroxyacyl coenzyme a dehydrogenase (hoad) in sceloporus undulatus (eastern fence lizard). the enzymes were assayed to indicate maximal catabolic activities that support exercise. parallel experiments were done on captive and field-active groups to determine wh ... | 1991 | 2026310 |
| the role of definitive radiation therapy in squamous cell carcinoma of the oral tongue. | between 1968 and 1985, 114 patients with squamous cell carcinoma of the tongue were evaluated in the department of radiation oncology at the university of virginia (charlottesville, va); of these, 86 received treatment with curative intent. the majority were treated with radiation therapy alone, whereas the remainder were treated with radiation therapy with preoperative or postoperative surgery. there were 17 t1 primary malignancies, 40 t2, 27 t3, and 2 t4. clinically positive adenopathy was pre ... | 1991 | 2025834 |
| periodontitis as an early presentation of hiv infection. | to determine whether the presence of rapidly progressive periodontitis (rpp) in people at high risk for acquired immunodeficiency syndrome (aids) may be the first symptom of previously unrecognized human immunodeficiency virus (hiv) infection. | 1991 | 2025822 |
| variation of the permeability of bacteriophage t4: analysis by use of a protein-specific probe for the t4 interior. | the permeability of bacteriophage t4 and the change in t4 permeability caused by mutation to osmotic shock resistance are investigated here by quantification of the kinetics with which both a dna-specific probe (ethidium) and a protein-specific probe [1,1'-bi(4-anilino)naphthalene-5,5'-di-sulfonic acid, or bis-ans)] bind to t4. in the case of an osmotic shock-resistant mutant, t40s41, both ethidium and bis-ans bind with first order kinetics. the first-order rate constant (k*) for both bis-ans an ... | 1991 | 2025682 |
| the pharmacodynamics of alcuronium in the elderly. | alcuronium (0.2 mg/kg) was given to 12 elderly patients, mean age 77 years (range 70-88 years) and 12 young patients, mean age 24 years (range 18-32 years) undergoing general anaesthesia. a compound muscle action potential was monitored continuously throughout anaesthesia, using an electromyograph and the train-of-four twitch technique. the rate of onset and maximum block achieved were similar in both the young and elderly patients, as were the times to 20% recovery of the first twitch compared ... | 1991 | 2024743 |
| protein-protein interactions with the acidic cooh terminus of the single-stranded dna-binding protein of the bacteriophage t4. | the single-stranded dna-binding protein of the bacteriophage t4 is encoded by gene 32. monoclonal antibodies were raised against intact gene 32 protein (gp32). we mapped the epitopes recognized by 12 of these monoclonal antibodies; the epitopes are all within the cooh-terminal region of gp32. as shown by others, removal of the cooh terminus of gp32 abolishes the ability of the intact protein to bind to many t4 proteins involved in replication, recombination, repair, and late transcription. these ... | 1991 | 2023949 |
| circulating growth hormone, insulin-like growth factor i, cortisol and free thyroxine in children with schistosomiasis with and without hepatic fibrosis. | serum insulin, growth hormone (gh), cortisol, free thyroxine (t4) and plasma insulin-like growth factor i(igf-i) concentrations were measured in 20 children suffering from schistosomiasis as well as 10 healthy age-matched controls. circulating gh and insulin levels were determined after an intravenous infusion of arginine hcl (10 per cent solution, 0.5 g/kg). children with schistosomal hepatic fibrosis (n = 10) had heights more than 2 sd below the mean for their age and sex. their circulating ig ... | 1991 | 2023299 |
| reiterated gene amplifications at specific short homology sequences in phage t4 produce hp17 mutants. | bacteriophage t4 gene 17 amplification mutants (hp17) selected by growth of gene 17 amber mutants on ochre suppressor strains of escherichia coli carry two to more than sixfold tandem head-to-tail repeats of the gene 17-18 region (wu & black, 1987). we characterized the structures of hp17 isolates by restriction enzyme mapping and southern blot analysis. the left and right boundaries of the amplified sequences were mapped within genes 16 and gene 18 or 19, respectively. the taqi-restriction frag ... | 1991 | 2023246 |
| hepatic 5'-deiodinase activity of japanese quail using reverse-t3 as substrate: assay validation, characterization, and developmental studies. | using rt3 as substrate, an in vitro 5'd assay was validated for use with liver tissue from adult japanese quail, by defining conditions under which activity is proportional to enzyme (protein) concentration and is linear with incubation time. activity was measured as the release of 125i from labeled rt3. using validated assay conditions we found the following 5'd characteristics: maximal activity from 10 to 50 mm dithiothreitol (cofactor), an apparent km of 0.52 microm rt3, ph optimum of 7.6-8.5 ... | 1991 | 2022947 |
| a prepubertal surge of thyrotropin precedes an increase in thyroxine and 3,5,3'-triiodothyronine in normal children. | the variations in plasma levels of tsh, t4, t3, and rt3, during the pubertal period, were studied in 647 school students from the urban area of santiago in chile (47% males and 53% females) with ages ranging between 7.5 and 15 yr. the subjects were grouped by age in consecutive intervals of 6 months each, and pubertal development was determined in every subject. tsh showed a significant increase, reaching a peak in the 9- to 9.5-yr interval. the same was found for t3 and t4, which reached a peak ... | 1991 | 2022717 |
| a study of the serum concentration of tumor necrosis factor-alpha in thyroidal and nonthyroidal illnesses. | we have studied serum concentrations of immunoassayable tumor necrosis factor-alpha (tnf alpha) and iodothyronines (t4, t3, and rt3) in normal subjects (n = 16) and patients with nonthyroidal illnesses (nti; n = 13), hyperthyroidism (n = 10), and hypothyroidism (n = 9). the mean (+/- sem; femtomoles per ml) serum concentration of tnf alpha was 45 +/- 4.3 in normal subjects, 84 +/- 38 in nti, 54 +/- 6.0 in hyperthyroidism, and 50 +/- 10 in hypothyroidism; the various values did not differ signifi ... | 1991 | 2022711 |
| a retrovirus-like zinc domain is essential for translational repression of bacteriophage t4 gene 32. | gene 32 protein (gp32), a single-stranded dna-binding protein from bacteriophage t4, contains a zinc-binding subdomain with sequence homologies to the 3-cysteine/1-histidine zinc-binding motif found in a variety of retroviruses and plant viruses. in vitro studies suggest that autoregulation of gp32 occurs at the level of translation by gp32 specifically binding gene 32 mrna at an unusual stem-loop structure that can be modeled as an rna pseudoknot. nucleation of gp32 binding via this pseudoknot ... | 1991 | 2022625 |
| induction of antigen-specific immune response with use of anti-idiotypic monoclonal antibodies to anti-carcinoembryonic antigen antibodies. | anti-idiotypic monoclonal antibodies (moabs) were prepared in a syngeneic system against anti-carcinoembryonic antigen (cea) moab 5b3 (igg1), which reacted with a carbohydrate moiety on cea, and moab ma208 (igg1), which reacted with a peptide on cea. anti-idiotypic moab t3-503 and t4-202 recognized the private idiotype of moab 5b3; anti-idiotypic moab m7-049 and m7-625 did so for moab ma208. idiotype mapping showed that moab 5b3 has at least two distinct idiotopes at its combining site and moab ... | 1991 | 2021940 |
| a conformational switch is associated with receptor affinity in peptides derived from the cd4-binding domain of gp120 from hiv i. | a 15-residue region within the cd4-binding domain of gp120 from hiv i was identified with use of folding algorithms as conserving the potential for forming a particular secondary structure throughout 11 sequenced hiv strains. the region chosen has a potential for forming both beta-sheet and alpha-helix; the helical form would be amphipathic with the five hydrophobic residues all totally or functionally conserved. five peptides were synthesized corresponding to this region in strain lav and the s ... | 1991 | 2021643 |
| dietary restriction on serum thyroid hormone levels. | to determine the long term effects of a protein sparing fast on serum thyroid hormone levels, the authors studied 38 obese patients ingesting a diet of 320 kcal for up to 13 weeks. the high baseline serum triiodothyronine (t3) levels decreased significantly by the first week, further decreased by the third week, and this lower level persisted for the duration of the fast until realimentation. serum free t3 index followed the same general pattern as did serum t3 levels. serum reverse t3 increased ... | 1991 | 2021153 |
| investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence. | eighteen monoclonal antibodies (mabs) putatively to non t4/t8 (null) cell antigens were tested by two-colour immunofluorescence and antibody binding inhibition (blocking), with one selected mab (cc15) that previous studies had indicated to be specific for null cells. none of the other mabs blocked binding of cc15 to lymphocytes. three main patterns of reaction were observed in two-colour immunofluorescence studies: mabs that stained the same cells as cc15, mabs that only stained a sub-population ... | 1991 | 2021062 |
| nimorazole as a hypoxic radiosensitizer in the treatment of supraglottic larynx and pharynx carcinoma. first report from the danish head and neck cancer study (dahanca) protocol 5-85. | between january 1986 and september 1990, 442 patients with pharynx and supraglottic larynx carcinoma were randomized to receive the hypoxic cell radiosensitizer nimorazole (nim) or placebo in association with a course of conventional primary radiotherapy. a preliminary analysis including the first 288 patients showed that the stratification parameters were significant (3-year actuarial local-regional tumor control, p less than 0.05) for sex (females 52% vs males 34%), tumor size (t1-t2 47% vs t3 ... | 1991 | 2020763 |
| dual start motif in two lambdoid s genes unrelated to lambda s. | the lysis gene region of phage 21 contains three overlapping reading frames, designated s21, r21, and rz21 on the basis of the analogy with the srrz gene cluster of phage lambda. the 71-codon s21 gene complements lambda sam7 for lysis function but shows no detectable homology with s lambda in the amino acid or nucleotide sequence. a highly related dna sequence from the bacteriophage pa-2 was found by computer search of the genbank data base. correction of this sequence by insertion of a single b ... | 1991 | 2019562 |
| 17beta-estradiol releases thyroxine from the thyroid follicles of a teleost, channa gachua (ham.). | to observe a direct effect of 17beta-estradiol (e2) on thyroid activity, thyroid follicles were isolated from hypobranchial muscles of a freshwater murrel, channa gachua. thyroid follicles were incubated (5 x 10(6) follicles/well) in vitro at 30 degrees for 2 hr without hormone and then 3 hr with e2 or bovine thyroid-stimulating hormone (btsh). addition of 10 and 100 ng of e2 to thyroid follicles resulted in 2- and 3-fold increases in thyroxine (t4) release. when 100 ng of btsh was added to chec ... | 1991 | 2019397 |
| effects of cortisol on aspects of 3,5,3'-triiodo-l-thyronine metabolism in rainbow trout (oncorhynchus mykiss). | aspects of 3,5,3'-triiodo-l-thyronine (t3) metabolism were studied in fed rainbow trout (oncorhynchus mykiss) held at 11.5-14 degrees and intraperitoneally implanted with hydrogenated corn oil (controls) or oil containing cortisol. cortisol implants caused dose-related plasma cortisol elevations within the physiological range for 2-3 weeks, loss in body weight, and depression in plasma t3 and free t3 index with no consistent change in plasma thyroxine (t4) or free t4 index. plasma t3 clearance r ... | 1991 | 2019395 |
| nucleolar organiser regions (nors) in bladder cancer; relation to histological grade, clinical stage and prognosis. | nucleolar organiser regions (nors), which are related to regulation of protein synthesis and cell proliferation, were estimated subjectively in 89 bladder cancer patients by means of silver staining technique on archival paraffin sections. the number of ag-nors was correlated to histological grade (who), clinical stage (uicc) and clinical behaviour of tumours during a mean follow-up period of 14 years. total number of ag-nors and the number of dispersed ag-nors were significantly related to hist ... | 1991 | 2018387 |
| rt3 metabolism in patients with nephrotic syndrome and normal gfr compared with normal subjects. | to evaluate reverse 3,3',5'-triiodothyronine (rt3) metabolism in nephrotic syndrome, serum rt3 kinetic studies from 10 nephrotics (mean urinary protein losses 7.0 g/day) with normal glomerular filtration rates (gfr; creatinine clearance 107 ml/min) were compared with 9 normal healthy subjects. serum disappearance data were analyzed in a three-pool model, including rapidly (liver and kidney) and slowly (muscle, skin, and brain) equilibrating pools exchanging with serum, with all losses from the r ... | 1991 | 2018127 |
| a novel dna joining activity catalyzed by t4 dna ligase. | the use of t4 and e. coli dna ligases in genetic engineering technology is usually associated with nick-closing activity in double stranded dna or ligation of 'sticky-ends' to produce recombinant dna molecules. we describe in this communication the ability of t4 dna ligase to catalyze intramolecular loop formation between annealed oligodeoxyribonucleotides wherein watson-crick base pairing is absent on one side of the ligation site. enzyme concentration, loop size, substrate specificity, and bas ... | 1991 | 2017363 |
| hepatic p450 expression in hypothyroid rats: differential responsiveness of male-specific p450 forms 2a (iiia2), 2c (iic11), and rlm2 (iia2) to thyroid hormone. | studies carried out in hypophysectomized adult rats have demonstrated that both thyroid hormone and gh can suppress hepatic expression of the steroid 6 beta-hydroxylase p450 2a (iiia2). the present study further characterizes the influence of thyroid hormone on the expression of p450 2a and two other male-specific hepatic p450s, a steroid 2 alpha/16 alpha-hydroxylase, designated p450 2c (iic11), and a steroid 15 alpha-hydroxylase, designated p450 rlm2 (iia2). these studies were carried out in ra ... | 1991 | 2017188 |
| gene product dsba of bacteriophage t4 binds to late promoters and enhances late transcription. | gene product 33 of phage t4 is known to be essential in late transcription. upstream from gene 33 and overlapping its 5' terminal sequence by 20 bp, we identified an open reading frame coding for a binding protein for double-stranded dna (dsba). gene product dsba is composed of 89 amino acid residues with a mr of 10376 kda. we purified this protein to homogeneity from over-expressing cells. gel retardation assays reveal that it binds to dna and footprint analyses disclose that it interacts prefe ... | 1991 | 2017138 |
| [a new in vivo radiation method for determining the stable iodine content of the thyroid]. | a new noninvasive method with a very low local radiation exposure (less than 2 msv) is based on the principle of recording and assessment with an externally located gage of characteristic radiation of stable iodine of the thyroid with the energy of 28 kev, excited by externally located sources of irradiation. the method was given the name of radiofluorescent assay (rfa) or x-fluorescent scanning of the thyroid. a series of units and methods of investigation were developed for rfa. the reproducib ... | 1991 | 2016999 |
| grading of human urothelial carcinoma based on nuclear atypia and mitotic frequency. ii. prognostic importance. | a grading system was applied in a retrospective study of 124 patients with transitional cell carcinoma of the bladder using a modified bergkvist system. the intermediate world health organization (who) grade ii tumors were divided into supposedly benign and malignant forms of grades iia and iib, respectively. the 5-year survival was 92% for patients with grade iia urothelial carcinoma, compared to 43% for patients with grade iib tumors. the corresponding figures for grades i and iii tumors were ... | 1991 | 2016812 |
| influence of nutritional deprivation on insulin-like growth factor i, somatotropin, and metabolic hormones in swine. | although growth hormone (gh) is a primary stimulus for the synthesis of insulin-like growth factor i (igf-i), other factors such as nutritional status, insulin, and thyroid hormones are important modulators of circulating igf-i levels. thus, the effects of feed restriction and subsequent refeeding on plasma levels of igf-i, gh, insulin, and thyroid hormones were studied in swine. despite an elevation in plasma gh levels after 48 h of feed restriction, circulating igf-i levels were decreased by 5 ... | 1991 | 2016201 |
| subcellular distribution of thyroxine 5'-monodeiodinase activity in rabbit kidney. | thyroxine 5'-monodeiodinase is located in the proximal tubules of the rabbit kidney. to estimate the subcellular distribution of 5'-monodeiodinase activity, we prepared subcellular fractions, a basolateral membrane fraction and a brush border membrane fraction, from kidneys of japanese white rabbits. each fraction (0.5 mg protein) was incubated at 37 degrees c for 60 min with 0.5 micrograms t4 in the presence of 5 mm dtt. the t3 generated in the reaction mixture was extracted with cold ethanol a ... | 1991 | 2016083 |
| [basedow's disease and thyroid stimulating hormone producing pituitary adenoma in a patient]. | tsh-producing adenomas of the pituitary gland are very rare. synchronous combinations of tsh-producing adenomas with other causes of hyperthyroidism are certainly extremely rare. we present the second known case, reported in the literature, consisting of observations for 12 years in a woman aged 43 years, who presented with active graves' disease and an apparently inactive pituitary macro-adenoma. however, after normalisation of serum t3 and serum t4 levels by antithyroid medication for one year ... | 1991 | 2014575 |
| a case of acetohexamide-induced hypoglycemia: the influence of hypothyroidism on the metabolism of acetohexamide. | prolonged hypoglycemia induced by acetohexamide (ah) in a patient with noninsulin dependent diabetes mellitus accompanied by primary hypothyroidism was presented. a 74-year-old man who had been treated with ah (500mg, daily) for diabetes mellitus since 1973 was admitted to our hospital in oct. 1988 because of hypoglycemic coma. on admission, the level of blood glucose was 20mg/dl. continuous intravenous administration of 10 per cent glucose solution led to improvement in the mental state on the ... | 1991 | 2013345 |