Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| characterization of the restriction site of a prokaryotic intron-encoded endonuclease. | the 1016-base-pair (bp) intron in the t4 bacteriophage thymidylate synthase gene (td) contains a 735-bp open reading frame that encodes a protein product with endonucleolytic activity. the endonuclease shows specificity for the intronless form of the td gene. highly purified endonuclease cleaves the dna of the intronless form of the td gene in vitro at 24 bp upstream of the exon 1-exon 2 junction, generating a 2-base staggered cut with 3'-hydroxyl overhangs. although the endonuclease cleaves in ... | 1990 | 2159153 |
| effects of the bacteriophage t4 gene 41 and gene 32 proteins on rna primer synthesis: coupling of leading- and lagging-strand dna synthesis at a replication fork. | we have demonstrated previously that the template sequences 5'-gtt-3' and 5'-gct-3' serve as necessary and sufficient signals for the initiation of new dna chains that start with pentaribonucleotide primers of sequence pppapcpnpnpn or pppgpcpnpnpn, respectively. normally, the complete t4 primosome, consisting of the t4 gene 41 (dna helicase) and gene 61 (primase) proteins, is required to produce rna primers. however, a high concentration of the 61 protein alone can prime dna chain starts from th ... | 1990 | 2158814 |
| response of phage t4 polynucleotide kinase toward dinucleotides containing apurinic sites: design of a 32p-postlabeling assay for apurinic sites in dna. | we have examined the capacity of bacteriophage t4 polynucleotide kinase (ec 2.7.1.78) to phosphorylate the partially depurinated products of d-apa, namely, d-spa and d-aps (where s represents an apurinic deoxyribose group). it was observed that the enzyme acted only on the latter isomer. since molecules of this type (d-nps) are the sole apurinic site containing products resulting from the combined digestion of lightly depurinated dna by snake venom phosphodiesterase and calf alkaline phosphatase ... | 1990 | 2158812 |
| cleavage specificity of bacteriophage t4 endonuclease vii and bacteriophage t7 endonuclease i on synthetic branch migratable holliday junctions. | holliday junctions are intermediate structures that are formed and resolved during the process of genetic recombination. to investigate the interaction of junction-resolving nucleases with synthetic holliday junctions that contain homologous arm sequences, we constructed substrates in which the junction point was free to branch migrate through 26 base-pairs of homology. in the absence of divalent cations, we found that both phage t4 endonuclease vii and phage t7 endonuclease i bound the syntheti ... | 1990 | 2158565 |
| inflammatory and non-inflammatory inclusion body myositis. characterization of the mononuclear cells and expression of the immunoreactive class i major histocompatibility complex product. | in ten patients with inclusion body myositis (ibm) five muscular biopsies showed profuse inflammatory exudates and three showed a few scattered inflammatory cells with partial invasion in some muscle fibers. no inflammatory cells were seen in two cases. in all patients, histopathological, histomorphometric and immunocytochemical studies were performed. immunocytochemistry for the class i and class ii major histocompatibility complex gene product (mhc) was performed in all cases and in ten contro ... | 1990 | 2158202 |
| physiologic thyroid activation in normal early pregnancy is induced by circulating hcg. | in normal early pregnancy, serum free thyroxine (t4) increases and serum tsh decreases, indicating that the thyroid gland is activated physiologically. to identify the factor responsible for this thyroid activation, we measured the serum thyroid-stimulating activity in comparison with the serum level of hcg in 39 normal women in early pregnancy. serum thyroid-stimulating activity was measured by a sensitive cyclic adenosine 3',5'-monophosphate (camp) accumulation assay using a rat thyroid cell l ... | 1990 | 2158026 |
| mutational alteration of the breakage/resealing subunit of bacteriophage t4 dna topoisomerase confers resistance to antitumor agent m-amsa. | bacteriophage t4 provides a simple model system in which to examine the mechanism of action of antitumor agents that have been proposed to attack type ii dna topoisomerases. prior results demonstrated that t4 type ii dna topoisomerase is the target of antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-amsa) in phage-infected escherichia coli: a point mutation in topoisomerase structural gene 39 was shown to confer both m-amsa-resistant phage growth and m-amsa-insensitive topoisome ... | 1990 | 2157956 |
| alterations in na+, k+ and ca2+ transport in erythrocytes of patients with carcinomas of the head and neck: possible role of mild anaemia and cell age. | 1. rates of erythrocyte ca2+ inward transport, activities of erythrocyte ca2(+)-adenosine triphosphatase, na+, k+ pump, na(+)-k+ co-transport and na(+)-li+ exchange, and erythrocyte ca2+, k+ and na+ contents have been determined in patients with carcinomas of the head and neck. 2. decreases in ca2+ inward transport and na(+)-k+ co-transport activity, and increases in erythrocyte ca2+ and k+ contents, were observed. 3. the more pronounced changes found in advanced carcinoma stages proved to be re ... | 1990 | 2156652 |
| effects of dna-binding drugs on t4 dna ligase. | a number of dna intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, amp-dependent dna topoisomerization and edta-induced dna nicking mediated by bacteriophage t4 dna ligase. the inhibition seems to arise from drug-substrate interaction so that formation of active dna-mg2(+)-amp-enzyme complex is impaired while assembled and active complexes are not disturbed by drug binding to the substrate. | 1990 | 2156493 |
| t lymphocyte subset alterations following bluetongue virus infection in sheep and cattle. | to determine potential mechanisms of differential disease expression in ruminants infected with bluetongue virus (btv), clinically normal, btv-seronegative, yearling sheep and cattle were infected subcutaneously with a standardized insect-source inoculum of btv serotype 17 (btv-17) (three infected and one contact control each) or animal adapted btv serotype 10 (btv-10) (three sheep only). btv was isolated from peripheral blood cell components of infected sheep and cattle and all infected animals ... | 1990 | 2156375 |
| the myod dna binding domain contains a recognition code for muscle-specific gene activation. | a 60 amino acid domain of the myogenic determination gene myod is necessary and sufficient for sequence-specific dna binding in vitro and myogenic conversion of transfected c3h10t1/2 cells. we show that a highly basic region, immediately upstream of the helix-loop-helix (hlh) oligomerization motif, is required for myod dna binding in vitro. replacing helix1, helix2, or the loop of myod with the analogous sequence of the drosophila t4 achaete-scute protein (required for peripheral neurogenesis) h ... | 1990 | 2155707 |
| stimulation by thyroid hormone of na+-h+ exchange activity in cultured opossum kidney cells. | cultured opossum kidney (ok) cells were used to determine whether thyroid hormone has a direct stimulatory effect on na(+)-h+ exchange activity in an intact cellular preparation. na+ uptake or intracellular ph recovery was measured in confluent monolayer cells following acid loading with nh4cl. triiodo-l-thyronine (t3) had no effect on cell number or protein and dna contents but stimulated amiloride-sensitive na+ uptake in a dose- and time-dependent manner. maximal stimulation of na+ uptake was ... | 1990 | 2155539 |
| lack of apparent difference in outcome between clinically staged iiia and iiib non-small-cell lung cancer treated with radiation therapy. | the current american joint committee on cancer (ajcc) staging system for bronchogenic carcinoma, which divides stage iii m0 cases into stages iiia and iiib, is based on the observation that selected patients with iiia disease (t3 or n2) can undergo complete surgical resection, in distinction to iiib patients (t4 or n3). to understand the value of this system when applied to clinically staged (cs) patients treated with a standard nonoperative approach, the records of patients with squamous cell, ... | 1990 | 2155312 |
| replacement of leu227 by pro in thyroxine-binding globulin (tbg) is associated with complete tbg deficiency in three of eight families with this inherited defect. | the t4-binding globulin (tbg) gene is a single copy located on the x-chromosome. previous studies have failed to elucidate the molecular defect in individuals with complete tbg deficiency (tbg-cd). indeed, no major deletions, insertions, or other rearrangements were observed in the tbg gene of six unrelated males with this defect. to clarify the molecular basis of tbg-cd, we have cloned and sequenced the tbg gene of an affected male (cd5) of french canadian origin. the sequence of the exons enco ... | 1990 | 2155256 |
| intermediary metabolism in a lizard, calotes versicolor: role of thyroid hormones. | in calotes versicolor, thyroidectomy did not alter the blood glucose level, lactate dehydrogenase (ldh liver and heart), acid phosphatase (ac.pase liver and kidney), and alkaline phosphatase (alk.pase liver and kidney) activities; significantly decreased the activities of glucose-6-phosphatase (g-6-pase liver and kidney), glutamic oxaloacetic transaminase (got liver and heart), glutamic pyruvic transaminase (gpt liver), and urea concentration (liver and kidney); and increased liver cholesterol w ... | 1990 | 2155152 |
| thyroid and adrenal function tests in adult male ferrets. | effects of thyroid-stimulating hormone (tsh) and thyrotropin-releasing hormone (trh) on plasma concentrations of thyroid hormones, and effects of acth and dexamethasone on plasma concentrations of cortisol, were studied in adult male ferrets. thirteen ferrets were randomly assigned to test or control groups of eight and five animals, respectively. combined (test + control groups) mean basal plasma thyroxine (t4) values were different between the trh (1.81 +/- 0.41 micrograms/dl, mean +/- sd) and ... | 1990 | 2154147 |
| supplementary iodine fails to reverse hypothyroidism in adolescents and adults with endemic cretinism. | the efficacy of supplemental iodine in correcting hypothyroidism in adults and older children with endemic myxedematous cretinism is not known. to investigate this issue we administered im iodized oil (1.5 ml) to 28 hypothyroid endemic cretins (tsh, greater than 5 miu/l) from western china, aged 14-52 yr (mean = 29 sd = 11 yr). clinical examination, intelligence testing (hiskey nebraska test of learning aptitude and the griffiths mental development scales), and thyroid function tests were perfor ... | 1990 | 2153694 |
| nonsquamous cell malignancies of the paranasal sinuses. | nonsquamous cell malignancies represent half of paranasal sinus cancers. since 1975, 103 patients have been treated at the cleveland clinic foundation for sinus malignancies. forty-nine patients had nonsquamous cell malignancies: 12 (25%) sarcoma, 10 (20%) adenoid cystic carcinoma, 9 (18%) adenocarcinoma, 9 (18%) lymphoma, and 9 (18%) miscellaneous tumors. ten patients presented with t1 or t2 disease at the primary site, 16 with t3, and 23 with t4. ten patients had primary ethmoid malignancies; ... | 1990 | 2153014 |
| hydrogen peroxide-induced dna damage in bovine lens epithelial cells. | the present investigation was undertaken to determine the types and extent of dna damage resulting from incubation of primary cultures of bovine lens epithelial cells with hydrogen peroxide. significant numbers of dna single-strand breaks were detected by alkaline elution after exposure to as little as 25 microm h2o2 for 5 min at 37 degrees c. the extent of single-strand breakage was concentration dependent and linear from 25 to 200 microm h2o2. the observed single-strand breaks appear primarily ... | 1990 | 2152969 |
| thyroid hormone regulates rat pituitary insulin-like growth factor-i receptors. | as we previously obtained evidence that insulin-like growth factor-i (igf-i) inhibits t3-induced gh secretion and gh mrna expression without affecting basal gh secretion in thyroidectomized rat pituitary cells grown in hypothyroid medium, we examined changes in igf-i receptors in the pituitary gland, as induced by thyroid hormone. thyroidectomized rats and a quantitative receptor autoradiographic method were used. the density of [125i]igf-i-binding sites in the anterior pituitary gland decreased ... | 1990 | 2152872 |
| [nodular goiter recurrent after surgery. a study of its pathogenesis and incidence]. | we analyzed 33 patients with recurring nodular goiter (from a population of 2,500 operated patients). recurrence occurred 0.7 to 12 years after surgery. t4, t3, tsh, perchlorate and trh tests were performed in 18. results were similar to those in 13 operated patients without recurrence. administration of thyroid hormone did not influence recurrence. seven patients with recurring goiter were re-operated on; an encapsulated follicular cancer was found in one. our results suggest that recurrence of ... | 1990 | 2152700 |
| [acute poisoning with thyroxine in children]. | two girls aged 5 and 14 years were admitted to hospital after ingestion of l-thyroxine in estimated doses of 72 micrograms.kg and 118 micrograms.kg in the preceding 4 and 8 hours, respectively. both had high serum t4 (19 and 20 micrograms/dl) and were asymptomatic at the time of admission and both were treated with iopanoic acid 3 g orally every 72 hours, in order to block the conversion of t4 into t3. serum levels of t4 were still elevated for up to ten days (8 and 16 micrograms/dl, respectivel ... | 1990 | 2152220 |
| phenotypic identification of t lymphocytes subset in recurrent aphthous stomatitis. | recurrent aphthous stomatitis (ras) is characterized by recurring ulcers in the oral mucosa as abnormality of the immune response, autoimmune or abnormal immunological reaction to antigens of oral bacteria. this study was conducted on 25 patients 15 suffering ras of the minor form and 10 of the major form and 15 student as control. the t lymphocytes were determined by the monoclonal antibodies (okt) using the indirect immunofluorescent technique of hoffman percentage of cells having surface fluo ... | 1990 | 2152028 |
| [comparative studies on antifertility mechanism and toxicology of tripterygium wilfordii monomer t4 and gossypol]. | inhibition of spermatogenesis and turnover of basic nuclear protein in late elongated spermatids of rat testis by tripterygium wilfordii monomer t4 was more obvious than that by gossypol. clustered positive deposits of gamma-gt, alp reaction were found in the inner edges of epididymal epithelium. head and tail separation, mid-piece coiling and axial fiber dislocation of epididymal spermatozoa in t4 treated rats were more obvious than those of gossypol-treated rats. mitochondrial ldh, ldh-x and m ... | 1990 | 2151263 |
| [the effect of three kinds of thyroid hormone preparations on serum thyroid hormone and tsh in primary hypothyroidism]. | thirty-eight cases of primary hypothyroidism were treated prospectively with three kinds of thyroid hormone preparations, including levothyroxine, l-triiodothyronine and dry thyroid preparation. a protocol of different dosages with same biologic effects was followed: 100 micrograms levothyroxine approximately 40-60 mg dry thyroid preparation approximately 30-40 micrograms l-triiodothyronine. during drug replacement treatment, the change serum t3 and tsh is more sensitive than that of serum t4 an ... | 1990 | 2151260 |
| role of the major capsid protein of phage t4 in dna packaging from structure-function and site-directed mutagenesis studies. | heat cleavage of asp-pro peptide bonds was used to probe the primary structures of the phage t4 major capsid protein precursor, gp23, its mature capsid form gp23*, and a dna-dependent atpase, called capsizyme. this analysis suggests that capsizyme is a gp23** resulting from the n-terminal processing found in gp23* as well as shortening at the c-terminus. photoaffinity labeling with azido-atp and bru-dna, followed by heat cleavage, suggests binding sites for these compounds toward the c-terminus ... | 1990 | 2150914 |
| [the isolation and structure of tripchlorolide (t4) from tripterygium wilfordii]. | a new compound named tripchlorolide was isolated from total glucosides (t ii) extracted from the woody part of the root of tripterygium wilfordii hook. f. tripchlorolide (t4) was obtained as white needle-like crystals, mp 256-258 degrees c. its molecular formula is c20h25o6cl. the structure of t4 was elucidated on the basis of spectral (uv, ir, ms, ftms/sci, 1h-nmr, 13c-nmr, 1h-1h cosy, 13c-1h cosy) analyses, x-ray and chemical evidence. in preliminary pharmacologic assay, tripchlorolide showed ... | 1990 | 2150350 |
| dopaminergic dysfunction in neonatal hypothyroidism: differential effects of gm1 ganglioside. | the effects of daily treatment with gm1 ganglioside (30 mg/kg s.c.) from birth to day 30, on striatal pre- and postsynaptic markers of the dopaminergic system in euthyroid- and 32 day-old hypothyroid rats were studied. the purpose was to assess whether gm1 could prevent the extensive, hypothyroidism-provoked impairment of dopaminergic neurotransmission. neonatal administration of gm1 well counteracted the hypothyroidism-related deficits in striatal synaptosomal uptake of [3h]dopamine and in memb ... | 1990 | 2149873 |
| a new extra long acting depot preparation of the lhrh analogue zoladex. first endocrinological and pharmacokinetic data in patients with advanced prostate cancer. | a new depot formulation of the lhrh analogue zoladex (goserelin acetate) has been developed which releases the drug over a period of at least 3 months as judged by measurement of drug content in depots at intervals after insertion in male rats and by the suppression of oestrogen secretion and oestrus in female rats. this formulation is based on the lactide/glycolide polymer system used for the standard 1-month zoladex depot, but the dose has been increased to 10.8 mg and the characteristics have ... | 1990 | 2149507 |
| the influence of blood chemistry on t4 and ft4i in major depression. | the pathophysiology underlying thyroid dysfunction in some depressed patients has not yet been determined. in order to clarify possible biochemical influences on thyroid regulation in these patients, we retrospectively examined several thyroid indices in charts from 81 depressed inpatients and 82 psychiatric controls. the depressed group had significantly higher t4 levels and free t4 index (ft4i), as well as lower chloride (cl) levels than controls. albumin (alb) also tended to be higher in the ... | 1990 | 2148335 |
| receptor-recognizing proteins of t-even type bacteriophages. the receptor-recognizing area of proteins 37 of phages t4 tuia and tuib. | escherichia coli phages of the t4 family (t4, tuia, tuib) recognize their cellular receptors by means of a c-terminal region of protein 37; a dimer of this polypeptide (1026 residues in t4) is located at the distal part of the long tail fibers. virions of the t2 family use protein 38 (which is attached to the free end of protein 37) for this purpose. the corresponding areas of genes 37 belonging to tuia and tuib were cloned and sequenced. comparison of the deduced protein primary structures, inc ... | 1990 | 2147721 |
| effects of recombinant human erythropoietin on t lymphocyte subsets in hemodialysis patients. | lymphocyte subpopulations were studied by immunofluorescence staining with monoclonal antibodies and laser flow cytometry in the blood of 23 hemodialysis patients before and after 3 months of treatment with recombinant human erythropoietin (rhepo). correction of anemia was accompanied by an increase in natural killer cells and a decrease in b lymphocytes. in the 11 patients (group 1) with a baseline helper/suppressor (t4/t8) ratio greater than or equal to 2, the latter significantly decreased fr ... | 1990 | 2147556 |
| [t-cell subsets, lymphocyte proliferation and il-2 level in patients with anterior uveitis]. | the authors used the okt monoclonal anti-t-lymphocyte antibodies to enumerate the t-cell subsets in the peripheral blood of 47 patients with anterior uveitis (au) by indirect immunofluorescence assay. the t-cell proliferative response to pha and il-2 production of the patients were also studied. the results were that the patients with au had higher percentage of okt4 + cells, and the t4 +/t8 + ratio increased. the percentage of okt4 + cells in patients with active au was lower than in patients w ... | 1990 | 2147408 |
| frameshift and double-amber mutations in the bacteriophage t4 uvsx gene: analysis of mutant uvsx proteins from infected cells. | the bacteriophage t4 uvsx gene encodes a 43 kda, single-stranded dna-dependent atpase, double-stranded dna-binding protein involved in dna recombination, repair and mutagenesis. mutants of uvsx have a dna-arrest phenotype and reduced burst size. western blot immunoassay of uvsx peptides made by a number of amber mutants revealed amber peptides ranging from 25-32 kda. wild-type uvsx protein was also detected in lysates of cells infected with uvsx amber mutants, suggesting that their mutations are ... | 1990 | 2146483 |
| phenotypes and functions of lymphocytes in congenital toxoplasmosis. | comparisons of lymphocyte surface phenotypes and functions were made among 25 pediatric patients with congenital toxoplasmosis (ages 1 month to 5 years) and 24 uninfected babies, a baby with postnatally acquired infection, and 6 uninfected, 7 recently infected, and 6 chronically infected adults. percentages of lymphocytes with b1, t11, t3, t4, t8, t6, b4, ia, nkh phenotypes and t4 to t8 ratios of infected and uninfected babies were the same (p greater than 0.05). lymphocytes from congenitally in ... | 1990 | 2146348 |
| failure of dehydroepiandrosterone to influence energy and protein metabolism in humans. | it was reported recently that 4 weeks of dehydroepiandrosterone (dhea) treatment [5.55 mmol/day (1600 mg/day), orally] reduced body fat and increased lean body mass in healthy men. the present study was performed to examine whether these effects could be explained by increased energy expenditure and muscle protein synthesis. eight healthy men were given placebo and dhea (1600 mg/day) for 4 weeks each in a double blind cross-over study. dhea treatment caused a 9-fold increase in mean plasma dhea ... | 1990 | 2146282 |
| atrial natriuretic factor in untreated hyperthyroidism. | increased plasma concentration of atrial natriuretic factor (anf) in untreated hyperthyroid patients is reported. a significant positive correlation between the concentration of anf and serum thyroid hormones (t4 and t3) has been found when hyperthyroid patients and healthy controls were pooled together. the mechanism by which thyroid hormones raise plasma anf concentration and its relevance to the symptomatology of hyperthyroidism is discussed. | 1990 | 2144955 |
| the uvsy protein of bacteriophage t4 modulates recombination-dependent dna synthesis in vitro. | an in vitro system containing the t4 gene 43, 45, 44/62, 32, dda, and uvsx proteins catalyzes dna synthesis that is dependent on the synapsis step of homologous genetic recombination. the rate of dna synthesis in this system is highly dependent on the concentration of the uvsx recombinase (a reca-like protein). here we report the effect of the t4 uvsy protein, a recombination accessory protein, on this reaction. low concentrations of uvsy protein greatly stimulate dna synthesis at low concentrat ... | 1990 | 2144282 |
| frequency analysis of human peripheral blood b cells producing igm-rheumatoid factor. differential effects of stimulation with monoclonal antibodies to cd3 and staphylococcus aureus. | the extent and nature of igm-rheumatoid factor (rf) precursors within normal human b cells were examined by utilizing two different polyclonal b cell stimulators, staphylococcus aureus cowan i (sa) and immobilized mab to the cd3 molecular complex (64.1). in cultures stimulated with sa, b cells produced igm-rf in the presence of t4 cells, factors generated from mitogen-activated t cells (tf), or il-2. similarly, in cultures stimulated with immobilized anti-cd3, t4 cells that had been treated with ... | 1990 | 2144005 |
| [preliminary studies of t lymphocyte subsets in patients with neurologic diseases]. | with immunofluorescence assay a preliminary study of t-cell subsets in the peripheral blood (pb) and cerebrospinal fluid (csf) of patients with multiple sclerosis (ms), viral encephalitis, guillain-barré syndrome (gbs), tuberculous meningitis, and other neurological diseases (ond) was carried out. the results revealed that the t4+ percentage and t4+/t8+ ratio of pb as well as csf increased, t8+ decreased in cases of ms. the changes of csf t subsets in cases of (gbs) were similar to those in case ... | 1990 | 2143977 |
| effects of hyper- and hypothyroidism on carbonic anhydrase, mg2(+)-dependent atpase and mg2(+)-dependent, hco3(-)-stimulated atpase activities of rat duodenal mucosa and kidney cortex. | carbonic anhydrase (ca) and mg2(+)-dependent atpase and mg2(+)-dependent, hco3(-)-stimulated atpase (mg2(+)-hco3(-)-atpase) activities in rat duodenal mucosa and kidney cortex were examined with respect to thyroidal status. administration of 50 and 150 micrograms thyroxine (t4)/kg per day s.c. for 7 days decreased duodenal cytosol ca activity to 66% of control with the former and 43% with the latter dose, while mg2(+)-hco3(-)-atpase activity in brush borders of duodenal mucosa was increased to 1 ... | 1990 | 2143214 |
| [immunoregulatory effects of monomer t4 from tripterygium wilfordii hook. i. immunoregulatory effects of t4 on nk activity of mouse splenocytes]. | effects of monomer t4 on nk activity of mouse splenocytes were determined both in vitro and in vivo by means of a classical i-udr release assay. the results showed that monomer t4 exhibited dose-dependent double-regulatory inhibiting enhancing effects, ie, t4 of low dosage enhanced the percentage of nk cytotoxicity and lu numbers, while a high dosage showed dose-dependent inhibition. the double-regulatory effects of monomer t4 might be important for evaluation of the therapeutic effects of tript ... | 1990 | 2143110 |
| structural studies of the contractile tail sheath protein of bacteriophage t4. 2. structural analyses of the tail sheath protein, gp18, by limited proteolysis, immunoblotting, and immunoelectron microscopy. | the molecular structure of the t4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, giving rise to stable protease-resistant fragments. limited proteolysis with trypsin gave rise to a trypsin-resistant fragment, ala82-lys316, with a molecular weight of 27k. chymotr ... | 1990 | 2143080 |
| structural studies of the contractile tail sheath protein of bacteriophage t4. 1. conformational change of the tail sheath upon contraction as probed by differential chemical modification. | differential chemical modifications of tyrosine residues of the tail sheath protein, gp18, were performed to elucidate the structural change of the tail sheath upon contraction. tyrosine residues of monomeric gp18, extended tail sheath, and contracted tail sheath were nitrated by tetranitromethane, and the modified tyrosine residues in each state of the sheath protein were identified by peptide mapping and amino acid sequence analyses of the isolated peptides. of 31 tyrosine residues in gp18 mon ... | 1990 | 2143079 |
| [changes in the thyroid function during treatment of psychiatric disorders using lithium salts]. | lithium, a monovalent cation used mainly in the management of certain psychiatric disorders, appears to act on thyroid function and to interfere with t3 and t4 secretion. the authors have followed 10 patients treated with lithium salts for two years and have monitored some parameters of thyroid function. clinical and laboratory signs of hypothyroidism appeared in one case and subsided after withdrawal of lithium treatment. | 1990 | 2142905 |
| [neoadjuvant chemotherapy using cisplatin (cddp) and methotrexate (mtx) in carcinoma of the bladder]. | from june 1986 to november 1989, 7 patients (pts.) with transitional bladder cancer were treated with cddp 70 mg/m2 i.v. on day 1 and mtx 40 mg/m2 i.v. on days 8 and 15. the initial stage was t2 n0 m0 (2), t2 n0 m0 (8), t4 n0 m0 (4) and t3-4 n+ m0 (3). the median age was 56 years. after a median number of two cycles (1-5) of cddp-mtx, 3/17 pts. (17.6%) had a complete remission (cm), 9/17 pts. (53%) a partial response (pr) greater than 50%, 4/17 pts. (23.4%) a pr less than 50%, 1/17 pts. (6%) a s ... | 1990 | 2142809 |
| [neoadjuvant chemotherapy of locally advanced tumors of the bladder: preliminary experience]. | from february 1988 to march 1989, 6 patients with locally advanced bladder cancer (t3b-t4, n0-n1, m0) were treated with 4 courses of neoadjuvant mp chemotherapy (methotrexate 300 mg/mq. + cisplatinum 100 mg/mq). in two patients chemotherapy was stopped because minimal or no response after two courses. partial response (rp) was achieved in three patients (50%). two patients died 2 and 5 month later. one patients developed metastases at 11 month. the remaining three cases showed ned at 3, 4 and 15 ... | 1990 | 2142808 |
| [neoadjuvant chemotherapy with mvc (methotrexate, vinblastine, cisplatin)in the treatment of infiltrating transitional carcinoma of the bladder]. | of 19 patients with advanced transitional bladder cancer (t2-t4, n0-n+, m0) who received two or three cycles of pre-emptive mvc (methotrexate, vinblastine, cisplatin), pathological partial (pr) and complete (cr) remissions were observed in 67% (50% and 17% respectively). the toxicity of chemotherapy was generally acceptable but 5 patients required hospitalization for neutropenia and thrombopenia . in one of them chemotherapy was stopped for severe sepsis. no death was observed. in 11 patients fo ... | 1990 | 2142807 |
| [neoadjuvant systemic chemotherapy with vmc (vinblastine, methotrexate and cisplatin) in locally advanced carcinoma of the bladder]. | a total of 25 patients with locally advanced bladder cancer, staged t3 and t4, have received neo-adjuvant vmc chemotherapy with vinblastine, methotrexate and cisplatinum. the evaluable patients were 21 and after cystectomy demonstrated the following responses: pcr in one case (4%), prp in ten cases (48%), pnc in 10 patients (48%). toxicity was mainly represented by leukopenia in 18 patients (82%), one of them died from neutropenic sepsis. after a median follow-up time of 12 months (range 6-39) 5 ... | 1990 | 2142804 |
| autoimmune thyroiditis associated with mild "subclinical" hypothyroidism in adults with down syndrome: a comparison of patients with and without manifestations of alzheimer disease. | serum tests of thyroid function were compared in down syndrome (ds) patients with and without manifestations of alzheimer disease (ad). relative to control individuals, ds patients had, overall, lower mean total t4 (p = 0.070) and t3f (p = 0.015), higher t3u (p = 0.013) and tsh (p = 0.020), no difference in free t4, and higher thyroid antithyroglobulin (ata) (p = 0.033) and antimicrosomal autoantibody (ama) titres (p = 0.0097). similar trends were apparent in ds males and females, and in ds pati ... | 1990 | 2142381 |
| hormonal effects of toremifene in breast cancer patients. | the effect of toremifene treatment on the serum levels of sex steroids (estradiol, progesterone, testosterone), fsh, lh, prolactin, tsh, t3, t4 and shbg was investigated. basal prolactin level and the "prolactin reserve capacity" of the hypophysis was also studied by the trh functional test. steroid hormone receptors were detected in the patients where a tumor biopsy could be obtained. in a randomized trial patients were treated by 60 and 300 mg of toremifene per os, daily. hormone levels were a ... | 1990 | 2142246 |
| mivacurium infusion requirements in pediatric surgical patients during nitrous oxide-halothane and during nitrous oxide-narcotic anesthesia. | we were interested in determining the infusion rate of mivacurium required to maintain approximately 95% neuromuscular blockade during nitrous oxide-halothane (0.8% end-tidal) or nitrous oxide-narcotic anesthesia. neuromuscular blockade was monitored by recording the electromyographic activity (datex nmt) of the adductor pollicis muscle resulting from supramaximal stimulation of the ulnar nerve at 2 hz for 2 s at 10-s intervals. mivacurium steady-state infusion requirements averaged 315 +/- 26 m ... | 1990 | 2141969 |
| adjuvant chemotherapy for medulloblastoma: the first multi-centre control trial of the international society of paediatric oncology (siop i). | two hundred and eighty-six patients with medulloblastoma from 46 centres in 15 countries were treated in a prospective randomized trial designed to assess the value of adjuvant chemotherapy. all patients were treated by craniospinal irradiation. those randomly allocated to receive adjuvant chemotherapy were given vincristine during irradiation and maintenance ccnu and vincristine, given in 6-weekly cycles, for 1 year. the overall survival was 53% at 5 years and 45% at 10 years. at the close of t ... | 1990 | 2141512 |
| studies on the role of the pituitary in the control of atrial natriuretic factor secretion and sodium excretion. | recent work suggests that hypophysectomized (hypox) rats show low levels of atrial natriuretic factor (anf) and an attenuated diuresis and natriuresis to blood volume expansion. the purpose of this was (i) to examine the effect of various hormone replacements on anf and renal excretion in hypox rats and (ii) to compare the renal responses to exogenous anf in intact and hypox rats. groups of rats received subcutaneous pellet implant of either dexamethasone (dex), thyroxine (t4), or a placebo. app ... | 1990 | 2141422 |
| t cell regulation of in vitro igm-rheumatoid factor production by normal human b cells. differential effects of stimulation with immobilized monoclonal antibodies to cd3 and staphylococcus aureus. | the roles of t cells in in vitro igm-rf production were examined utilizing two different polyclonal b cell stimulators, staphylococcus aureus cowan i (sa) and immobilized mab to the cd3 molecular complexes (64.1). in cultures stimulated with sa, t4 cells alone but not t8 cells alone could support igm-rf production. this function of t4 cells could be replaced by il2 alone or factors generated from mitogen activated t cells (tf). moreover, when il2 was supplemented to the cultures, t8 cells as wel ... | 1990 | 2141251 |
| down's syndrome and thyroid disorder. | the thyroid status of 106 adults with down's syndrome was assessed. six were previously diagnosed as hypothyroid and were already receiving thyroxine. a further 37 patients showed abnormal thyroid function. biochemical evidence of hypothyroidism (t4 less than 50 nmol/l and t.s.h. greater than 4 mu/less than) was found in one person. six patients were found to have an unequivocally elevated t.s.h. but normal t4 (t4 greater than 50 nmol/l and t.s.h. greater than 20 mu/l) and 29 were found to have ... | 1990 | 2140417 |
| [changes in t-cell subpopulations in normal pregnancy and in patients with spontaneous abortion]. | from the immunological point of view, pregnancy is a privileged allograft, with complex mechanisms of adaptation within the maternal immune system preventing rejection. the importance of lymphocytes and their specific subsets for this mechanism is a controversial issue. this study was designed to examine changes within t-cell subsets during uneventful pregnancy and to evaluate their significance by comparison with findings in patients undergoing spontaneous pregnancy loss. when compared to non-p ... | 1990 | 2140333 |
| lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus. | the lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (rsv) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. experimental infection with bovine rsv was characterized by a significant rise in sbu-t8+ (cd8+ or cytotoxic) t cells and a significant reduction in sbu-t4+ (cd4+ or helper) t cells and b (lca p220+) lymphocytes (p less than 0.05). the helper/s ... | 1990 | 2140217 |
| changes in lymphocyte subpopulations after prolonged exercise. | in order to assess the changes in the cellular immune system, 27 healthy male subjects were selected who participate in leisure-time sports and in whom measurements of the lymphocyte subsets in venous blood were carried out before and after a standardized endurance exercise test (ei, 60 minutes in length, intensity: 63% of maximum performance). the percentages of the following parameters were measured: total t-lymphocytes, b-lymphocytes and o-lymphocytes as well as t4-lymphocytes (helper t-lymph ... | 1990 | 2140110 |
| thyroid hormone replacement and growth of children with subclinical hypothyroidism and diabetes. | growth potential among people with type 1 diabetes and subclinical hypothyroidism may be significantly reduced. growth was evaluated in 25 children with diabetes who had thyromegaly and elevated thyrotrophin (tsh) levels. all patients appeared clinically euthyroid except for four with short stature. basal growth rate was significantly lower (p less than 0.005) in group 1 (tsh greater than 50 mu l-1) and group 2 (tsh level 10.1-50 mu l-1) than in patients with tsh levels between 5 and 10 mu l-1 ( ... | 1990 | 2140081 |
| expansion of a lymphocyte population co-expressing t4 (cd4) and t8 (cd8) antigens in the peripheral blood of a normal adult male. | approximately 2% to 3% of circulating human t cells co-express cd4 and cd8 (cd4+, cd8+) t-cell antigens. these cd4+, cd8+ cells may be immature precursors that function to replenish functional t-cell subsets. we detected a very high level of cd4+, cd8+ cells in the peripheral blood lymphocytes of a healthy white male, ranging from 21% to 36%. the morphology of his peripheral blood lymphocytes was normal, and he has maintained an elevated level of cd4+, cd8+ cells without clinical disease over a ... | 1990 | 2140062 |
| skin diseases in children with hiv infection and their association with degree of immunosuppression. | since the recognition of the acquired immunodeficiency syndrome (aids) in 1981, several dermatologic manifestations have been associated with the syndrome, including candidiasis, dermatophyte infection, molluscum contagiosum, herpes simplex and zoster, bacterial infection, and malignancy. skin diseases have been observed in both the adult and the pediatric human immunodeficiency virus (hiv) infection in an academic hospital setting in relationship to the current cdc classification of pediatric h ... | 1990 | 2139434 |
| altered thyroidal status and the in vivo synthesis of atrial natriuretic peptide in the rat heart. | the effect of altered thyroidal status on levels of immunoreactive (ir)- atrial natriuretic peptide (anp) in serum and the four cardiac chambers, and of tissue anp mrna, was determined in groups of rats given vehicle, thyroxine (t4), propylthiouracil (ptu) or t4 plus ptu for 3 weeks. serum levels of ir-anp were approximately 3-fold higher in t4-treated animals compared with control; levels in ptu or ptu/t4 groups were not different from control. right ventricular anp mrna was below detection; in ... | 1990 | 2139421 |
| expression and regulation of genes coding for three bacteriophage t4 tail tube-associated proteins. | the assembly and length regulation of the tail tube of bacteriophage t4 requires the function of three proteins: gp29, 48, and 54. six copies of each protein are found in the completed tail, and the genes for these proteins are adjacent on the t4 genome. evidence is presented here that gp54 is also a tail tube-associated protein that remains bound to the tail tube after the baseplate is removed by guanidine hydrochloride, suggesting that all three proteins interact structurally. there is a stron ... | 1990 | 2139268 |
| immunological features of human immunodeficiency virus disease. | infection with the human immunodeficiency viruses results in a profound immunosuppression responsible for most of the clinical features of aids. the virus devastates the immune system because its main target is the t4 lymphocyte, which is the key component for generating and regulating the immune response. the cellular receptor for hiv, the membrane glycoprotein cd4, is found mainly on the surface of this major subpopulation of t lymphocytes and also on many other cell types such as those of the ... | 1990 | 2138920 |
| cutaneous t cell lymphoma with suppressor/cytotoxic (cd8) phenotype: identification of rapidly progressive and chronic subtypes. | we identified nine patients with cutaneous t cell lymphoma in whom the neoplastic cells expressed the cd8 (t8) suppressor t cell phenotype instead of the more common cd4 (t4) helper t cell phenotype. five had rapidly progressive disease characterized by distinctive papulonodular skin lesions (four patients), involvement of palms or soles (four patients) or oral cavity (two patients), and poor response to standard topical therapy (four patients). histologic examination showed extensive epidermotr ... | 1990 | 2138636 |
| influence of cyclic interferon gamma on lymphocytes and their subpopulations in patients with renal carcinoma. | natural killer (nk) cell stimulation can be repeatedly elicited by cyclic administration of recombinant human interferon (rhu ifn) gamma in patients with metastatic renal cell cancer. in all cycles of treatment, the elevation of the cytolytic activity of the nk cells was statistically significant. the shift of the t4/t8 ratio (t-helper/t-suppressor lymphocytes) a short time after the commencement of ifn application might be a manifestation of the immunostimulation induced. | 1990 | 2137658 |
| unusual lymphocyte subset distribution in some depressed patients. | depression has been previously associated with immunologic dysfunction. the lymphocyte subsets of 10 patients with major depression were found to show a mean t4-t8 (helper-suppressor) ratio lower than that of controls, a difference attributable to increased numbers of suppressor cells in the depressed group. one possible cause of increased suppressor cells is prior or current viral infection. | 1990 | 2137122 |
| enzymatic formation and resolution of holliday junctions in vitro. | e. coli reca protein promotes homologous pairing and reciprocal strand exchange reactions between duplex dna molecules in vitro. reaction intermediates contain holliday junctions that are driven along the dna at a maximal rate approaching 1000 bases per minute. t4 endonuclease vii cleaves holliday junctions in vitro, and its inclusion in reca-mediated reactions leads to the rapid formation of heteroduplex products. product analysis indicates patch and splice recombinant molecules similar to thos ... | 1990 | 2137030 |
| alterations in rat cardiac myosin isozymes induced by whole-body irradiation are prevented by 3,5,3'-l-triiodothyronine. | changes in cardiac myosin isozymes and serum thyroid hormone levels were investigated in rats following 10 gy whole-body gamma irradiation. the percent beta-myosin heavy chain increased from 21.3 +/- 1.8 to 28.1 +/- 6.8 (ns) at 3-day postirradiation, 37.7 +/- 1.9 (p less than .001) at 6-day postirradiation, and 43.8 +/- 3.3 (p less than .001) at 9-day postirradiation. along with the change in myosin isozymes was a significant 53% decrease (p less than .001) in the serum thyroxine (t4) level by d ... | 1990 | 2136761 |
| cloning, sequence analysis, and expression of the bacteriophage t4 cd gene. | the cd gene of bacteriophage t4, which encodes the enzyme deoxycytidylate deaminase, was isolated as a 1.9-kilobase dna fragment and completely sequenced. the deduced amino acid sequence was found to be 193 residues long compared with 188 for the corresponding enzyme from bacteriophage t2. there were nine amino acid differences between the two enzymes in addition to a 5-residue insert near the carboxyl terminus of the t4 deaminase which was not present in the t2 deaminase. the cd-containing frag ... | 1990 | 2136740 |
| stimulation of rat atrial natriuretic peptide (ranp) synthesis by triiodothyronine and thyroxine (t4): t4 as a prohormone in synthesizing ranp. | thyroid hormones have been reported to increase the secretion and synthesis of atrial natriuretic peptide (anp) in vitro. in this study, we focused on the role of type i t4 5'-deiodinase to investigate the stimulating effects of t4 on anp synthesis and secretion by measuring cellular content and secretion into the medium of immunoreactive rat anp (ir-ranp) and ranp mrna levels in cultured rat neonatal atrial myocytes. both t3 (10(-9)-10(-7) m) and t4 (10(-8)-10(-6) m) increased cellular content ... | 1990 | 2136727 |
| the influence of pinealectomy and single dose of melatonin administered at different times of day on serum t3 and t4 concentrations in rats. | the influence of pinealectomy and melatonin administration on the levels of total t3 and t4 plasma levels were determined by ria method. the results of hormonal measurements were evaluated by means of student's t-test, and the diurnal rhythm by cosinor method. it has been established that pinealectomy disturbs the t3 diurnal rhythm and caused marked changes in t4 levels during the 24 hours. exogenous melatonin does not influence the thyroid function during the light phase. during the dark phase, ... | 1990 | 2136306 |
| direct and indirect techniques for free thyroxin compared in patients with nonthyroidal illness. iii. analysis of interference variables by stepwise regression. | we applied stepwise regression for multivariate analysis of data for free thyroxin (ft4) in serum and for other laboratory tests of thyroid function in patients with nonthyroidal illness. using the maximum r2 improvement and backward elimination methods to test five variables [prealbumin, albumin, t4-binding globulin (tbg), free fatty acids (ffa), and ffa/albumin molar ratio], we found that the variables with the greatest predictive power clustered according to the methodology of ft4 measurement ... | 1990 | 2136246 |
| c-eeg brain mapping in dsm-iii schizophrenics after acute and chronic haloperidol treatment. | a multi-lead c-eeg investigation was carried out, in order to evaluate changes induced by acute and chronic treatment with haloperidol in dsm-iii-r schizophrenics. after the acute treatment the main c-eeg changes were (1) a significant decrease of delta relative power (rp) over all the explored leads and of theta 1 over the occipital leads; (2) an increase in alpha 2 and beta 2 rp, as well as a decrease of beta 3 rp confined to the anterior temporal leads (t3, t4). during chronic treatment, c-ee ... | 1990 | 2136214 |
| variations in intrathyroidal lithium content and their effect on the iodide uptake in mouse thyroid. | lithium (li) is accumulated in the thyroid but the mechanism of li accumulation is not known. in the present study, the causes of variation in li concentration in the thyroid and the relation between cellular li and iodide were examined. this was done by using mice treated with li (0.01% as li2co3) for 4 weeks, co-administered with propylthiouracil (ptu, 0.5 mg/ml daily p.o.) or thyroxine (t4, 0.5 micrograms per day i.p.) for last 10 days. the total content of li in a whole thyroid (ng/thyroid) ... | 1990 | 2136134 |
| [limitations on the practice of head-and-neck oncology in africa. the example of laryngeal cancers]. | at the present time, cervico-facial oncology is one of the great themes of oto-rhino-laryngologists. cancer of the larynx has been chosen by the authors to demonstrate the limits in the practice of cervico-facial oncology in the tropics. over a period of 10 years, 61 patients with suspected malignant larynx afflictions were examined at dakar u.h.c.o.r.l. clinic. histological confirmation concerned 38 patients, and 27 underwent treatment aimed at curing them. as far as results are concerned, ther ... | 1990 | 2135803 |
| serum hormones in male epileptic patients receiving anticonvulsant medication. | circulating sex and thyroid hormones, as well as the pituitary function, were assessed in 63 male patients with epilepsy receiving either a single medication of carbamazepine, phenytoin, or valproate or a combination of carbamazepine plus phenytoin or carbamazepine plus valproate. all therapeutic regimens, including carbamazepine and/or phenytoin were associated with low levels of circulating thyroxine (t4), free thyroxine (ft4), and dehydroepiandrosterone sulfate, and with low values for the fr ... | 1990 | 2135734 |
| diagnosis of familial dysalbuminemic hyperthyroxinemia and investigation of the nature of the variant albumin. | a variant albumin with abnormally increased thyroxine-binding affinities has been identified, and termed as familial dysalbuminemic hyperthyroxinemia (fdh). the aims of this study are to seek for a method of diagnosis to distinguish fdh from other causes of hyperthyroxinemia and to investigate the nature of this abnormal albumin. percent t4 bound to albumin was high in fdh, ranging 29-48% (normal range 3-8%), as measured in [125i] t4 loaded serum using a single immunoprecipitation by anti-human ... | 1990 | 2135643 |
| canine pseudohypothyroidism and covert hypothyroidism. | primary hypothyroidism is perhaps the most common endocrinopathy of the dog. unfortunately, a paradox exists. many cases of mild to moderately severe hypothyroidism are unrecognized, while many euthyroid dogs are misdiagnosed as having hypothyroidism. uncommon manifestations resulting from earlier stages of primary hypothyroidism, from secondary/tertiary hypothyroidism, or from a unique interplay between thyroid hormone deficiency and the age, sex, or breed affected can obscure the clinical susp ... | 1990 | 2134082 |
| diagnosis of occult hyperthyroidism in cats. | as expertise among small animal practitioners grows, feline hyperthyroidism is being diagnosed earlier in the course of the disease. there are, in fact, a growing number of cats with clinical signs of hyperthyroidism and palpably large thyroid glands whose serum total thyroxine (t4) and triiodothyronine (t3) concentrations are within the normal or borderline range. this condition can be referred to as "occult" hyperthyroidism. early detection and treatment of feline hyperthyroidism presents an o ... | 1990 | 2134081 |
| [molecular biology of the genetic defects of synthesis, transport and peripheral action of the thyroid gland hormones]. | recent advances in molecular biology and the use of modern techniques made possible the identification and cloning of dna portions of the human genome encoding proteins that are important for the synthesis, storage, transport and action of thyroid hormones. these achievements have led to the diagnosis of the basic defects at the dna level and the understanding of the biochemical disturbance in the genetic disorders of thyroid metabolism. in patients with defective expression of thyroid peroxidas ... | 1990 | 2133168 |
| [subacute necrotizing lymphadenitis (kikuchi and fugimoto's disease)]. | subacute necrotizing lymphadenitis preferently or exclusively located in the cervical area is often unilateral, self-limited and of probable viral origin. women under 30 years of age are affected in 80% of cases. cure is produced in 2 to 3 months although sometimes a much longer course may be observed. recurrence in the same or other areas may also occur. the histological pattern is characteristic with foci of necrosis with dust-like nuclear rests, absence of neutrophils, scarcity of plasma cell ... | 1990 | 2133153 |
| [problems in the diagnosis of thyroid dysfunction of the elderly adult]. | thyroid disfunction in the aged is often misdiagnosed either due to scanty symptoms, masking by other ailments or because function tests can be altered by extrathyroid causes such as chronic diseases, drugs or undernutrition. we surveyed 93 patients from 60 to 104 years old (73 females) living in geriatric homes. most received at least 2 drugs for control of hypertension, coronary artery disease, diabetes, parkinsonism or psycho-organic deterioration. no clinical evidence of thyroid disfunction ... | 1990 | 2133150 |
| [thyroid disease in progressive systemic sclerosis]. | there is no agreement wither thyroid disease is a common feature of progressive systemic sclerosis (pss). we evaluated 28 patients with proven pss with determinations of t3, t4, tsh and tg as well as antimicrosomal autoantibodies. radioactive i uptake, thyroid scintigraphy, trh test and fine needle biopsy were performed in selected patients. twelve (43%) patients had clinical or laboratory evidence of thyroid dysfunction. four patients had euthyroid goiter, 3 had evidence of autoimmune disease, ... | 1990 | 2133148 |
| comparison of different methods for iodothyronines extraction from animal tissues. | four different methods for thyroid hormones concentration in various tissues have been compared. the methods involve homogenization, extraction with 95% ethanol and assay of iodothyronines by a highly sensitive ria. the method described by chopra et al. has been selected and used for further estimations as the most reliable and convenient for a continuous laboratory work. the t4 levels (means +/- se) found in the liver, kidney and skeletal muscle were 22.49 +/- 2.19, 14.89 +/- 1.56 and 8.33 +/- ... | 1990 | 2132659 |
| some extrathyroid regulatory mechanisms' aspects in thyroid humoral state at the delivery. | thyroid function parameters (triiodothyronine, thyroxine, reverse triiodothyronine, thyroid stimulating hormone, thyroglobulin) and thyroid binding globulin (tbg) were determined in sera of 64 women who had carried a normal pregnancy and delivered at term, as well as in sera of their newborns. obtained results were compared to the findings of the same parameters in 28 women who delivered at term, but had been receiving gestanges 1 to 5 months prior to the delivery, and in their babies. in both g ... | 1990 | 2132426 |
| [effect of electromagnetic radiation on t-lymphocyte subpopulations and immunoglobulin level in human blood serum after occupational exposure]. | the study was aimed at evaluation of the total lymphocyte, the t lymphocyte (t3), the t helper (t4) and the t suppressor (t8) count in the peripheral blood as well as of the igg, iga and igm level in sera of 39 radar operators aged from 20 to 22 years. the operators were exposed to electromagnetic radiation at frequencies ranging from 390 mhz to 10.96 ghz and power from 500 kw to 1.5 mw for a period from 720 to 7560 hours. as compared to the control group in the radar operators a statistically s ... | 1990 | 2131394 |
| cascade of overlapping late genes in bacteriophage t4. | the dna sequences of genes 9, 10, 11, 12, and wac, which encode the structural proteins in the bacteriophage t4 base plate, were determined. these genes form a single operon which is transcribed in a clockwise direction from a single late promoter in the tataaata region located upstream of gene 9 at position -10. a feature of the operon is an overlap between the termination codon of each upstream gene and the initiation codon of its downstream gene. with the exception of gene 10, the open readin ... | 1990 | 2130919 |
| [hereditary factors in the etiology of chronic lymphocytic leukemia]. | leukocyte counts, hemoglobin concentration, pas positivity index in lymphocytes, leukocyte alkaline phosphatase, karyotype, hla phenotype as wall as the quality of the cellular and humoral immunity have been studied in 45 patients with chronic lymphocytic leukemia (cll) and in 103 their closest relatives (sibs, parents). the aim was to detect the possible preleukemic condition in the relatives as a strong propensity towards developing has been previously established in families of patients with ... | 1990 | 2130026 |
| characterization of peripheral blood lymphocytes in patients with non-cirrhotic portal fibrosis: a comparison with cirrhotics and healthy controls. | non-cirrhotic portal fibrosis (ncpf) is a common cause of portal hypertension in india. its aetiopathogenesis is not well understood. to investigate the possibility of an immune mediated injury, phenotypes of peripheral blood lymphocytes were studied in 15 ncpf patients and were compared with an equal number of compensated cirrhotics and matched healthy controls. the population of total peripheral t lymphocytes (t1) and suppressor/cytotoxic phenotype (t8) was significantly (p less than 0.01) dec ... | 1990 | 2129827 |
| immunological profile of patients with non-cirrhotic portal fibrosis. | the aetiopathogenesis of non-cirrhotic portal fibrosis (ncpf), a common cause of portal hypertension in india, is not known. to study the immune status of ncpf patients and to see whether immunological mechanisms have a role to play, humoral and cell-mediated immunological studies were carried out in 43 patients with ncpf and compared with equal number of matched healthy controls and 31 patients with compensated liver cirrhosis. serum immunoglobulin a (iga) and complement (c3, c4) levels were si ... | 1990 | 2129814 |
| [changes in serum thyroglobulin levels during the trh test in euthyroid female patients with diffuse and nodular goiters]. | concentrations of thyroglobulin (tg) in the sera of 48 euthyroid females of different ages suffering from diffuse and nodular goitre during trh test, were determined. the aim of this study was to examine the correlation between the increased tsh levels and tg, and thyroid hormones in the serum in dependence of pathomorphological thyroid status and the patients' ages. tsh maximal level reached (+314%) compared with the basal level 25 minutes after trh injection, while max tg level was found 60 mi ... | 1990 | 2129364 |
| thyroid hormone elevations during acute psychiatric illness: relationship to severity and distinction from hyperthyroidism. | acute psychiatric illness may be accompanied by transient hyperthyroxinemia. the mechanism of this phenomenon was examined by determining the role of thyrotropin (tsh) in the genesis of this state. serial measurements of tsh, thyroxine (t4), free t4 index (ft4i), triiodothyronine (t3), and free t3 index (ft3i) were performed in 45 acutely hospitalized patients with major psychiatric disorders. twenty-two (49%) patients exhibited significant elevations (greater than or equal to 2 sd above mean va ... | 1990 | 2129342 |
| effects of trh and prolactin in the behavioral despair (swim) model of depression in rats. | the neuropeptides thyrotropin releasing hormone (trh) and prolactin (prl), which affect various behaviors in animals, showed "antidepressant" properties in an experimental model of depression. subcutaneous administration of trh reduced the total immobility time of rats tested in the despair (constrained swim) test and potentiated the anti-immobility effect of intraperitoneally administered desimipramine (dmi). this effect was not mimicked by the peripheral injection of tsh, t3 or t4. hyperprolac ... | 1990 | 2129310 |
| construction of yeast trna(tyr) derivatives lacking in large part of the molecule and their tyrosine acceptance. | derivatives of yeast trna(tyr) lacking in the anticodon-arm (and d-arm) were constructed by a combination of partial digestion with rnase t1 and joining with t4 rna ligase. aminoacylation analyses of these derivatives ("3/4 molecule" or "1/2 molecule") showed that sufficient information for binding to tyrrs is contained mainly in the aminoacyl-stem (and t psi c-arm) of yeast trna(tyr), but further information, possibly in the anticodon-arm, is necessary for efficient acceptance of tyrosine. | 1990 | 2129305 |
| a rise in haemoglobin levels may enhance serum triiodothyronine (t3). concentrations in prepubertal patients with beta-thalassaemia major. | the aim of the present study was to investigate the effects of increased haemoglobin (hb) levels on the thyroid function in patients with beta-thalassaemia major. basal levels of thyroid hormones (t4, t3) and free thyroid hormones (ft4, ft3), basal tsh concentrations and the tsh responses to a trh bolus (0.2 mg iv) were studied in ten euthyroid thalassaemic patients, aged 8 to 19 years, and in one 12 years-old thalassaemic girl with primary hypothyroidism. five euthyroid thalassaemic patients (a ... | 1990 | 2129031 |
| a case of hypersensitivity to thyroid hormones with normally functioning thyroid gland and increased nuclear triiodothyronine receptors. | a 52-year-old male presented himself with tachycardia crises which appeared first during childhood, increased in frequency without goiter or exophthalmos. cardiac and adrenergic diseases were excluded. the thyroid function was normal regarding t4, free t4 and t3, tbg, radioiodine uptake, tsh and t3 suppressibility; however the tsh response to trh was decreased. the lymphocyte nuclear t3 receptor was found with an affinity close to that of normal volunteers (ka: 1.42 x 10(10) m-1 vs 1.95 +/- 0.35 ... | 1990 | 2128940 |
| lithium carbonate therapy for induction of euthyroid state in thyrotoxicosis. a preliminary study. | lithium carbonate was tried in 27 patients with graves' disease to induce euthyroid state. each patient received 1200 mg of lithium carbonate in three divided doses. the study protocol included monthly monitoring of serum lithium, total serum t3, t4 and t3/t4 ratio, and a repeat radioactive iodine uptake (raiu) at the end of three months. twenty three patients completed the study. of these, only 9 (39.1%) achieved euthyroid state, with a significant reduction in serum t3 and t4 but not in t3/t4 ... | 1990 | 2128939 |
| effects of thyroid hormone, trh and tsh on pro-trh concentrations in various organs of rats. | the effects of large doses of thyroid hormone, thyrotropin (tsh) or thyrotropin-releasing hormone (trh) on pro-trh concentrations in the rat hypothalamus, cerebrum, cerebellum, brain stem, stomach and eye were studied. the rats were administered t4 (2.5 mg/kg), t3 (375 micrograms/kg), trh (1.0 mg/kg) or bovine tsh (1.25 iu/kg) i.p. and seven rats in each subgroup were decapitated at 4 or 24 h after the injection. pro-trh, trh, tsh and thyroid hormone were measured by each radioimmunoassay. immun ... | 1990 | 2128932 |