Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the therapeutic efficacy of oral cholecystographic agent (iopanoic acid) in the management of hyperthyroidism. | five randomly chosen patients with thyrotoxicosis were administered 1 gm of the oral cholecystographic agent iopanoic acid daily for 21 days. there was a marked fall in t3 levels by 75% of the pretherapy value by 96 hr; values remained normal over the 21-day period. t4 values fell significantly by seven days of therapy, and the decreased values were sustained. ft3 and ft4i also showed corresponding decreases in value. all subjects showed clinical improvement by both subjective and objective crit ... | 1990 | 2194004 |
| a new chemiluminescence immunoassay for triiodothyronine and thyroxine: evaluation using quality control sera assayed in an interlaboratory survey. | a recently developed chemiluminescence immunoassay system (lia-mat) for triiodothyronine and thyroxine, set up by byk-sangtec diagnostica (dietzenbach, germany), has been evaluated and compared with radioimmunoassays and with a chemiluminescence enhanced enzyme immunoassay (amerlite), using control materials circulated in a national interlaboratory quality control, as well as patient sera. the lia-mat assays are competitive methods which use coated monoclonal antibodies and triiodothyronine- or ... | 1990 | 2193102 |
| in vitro and in vivo activities of t4 endonuclease v mutants altered in the c-terminal aromatic region. | genes encoding mutants of the thymine photodimer repair enzyme from bacteriophage t4 (t4 endonuclease v) having an amino acid substitution (t127m, w128a, w128s, y129a, k130l, y131a, y132a) were constructed by use of a previously obtained synthetic gene and expressed in escherichia coli under the control of the e. coli tryptophan promoter. an in vitro assay of partially fractionated mutant proteins for glycosylase activity was performed with chemically synthesized substrates containing a thymine ... | 1990 | 2191719 |
| [squamous cell carcinoma-associated antigen (scc) as a tumor marker in the initial diagnosis of carcinomas of the head and neck region. results of a prospective study after 24 months]. | the serum--scc antigen levels of patients with head and neck tumors were studied prospectively to determine their value in the initial diagnosis of head- and neck-cancer patients. serum concentrations above 2 ng/ml are considered abnormal. preliminary results of the study after a 12-month period have been reported elsewhere (1). the final results of the study show an increased percentage (53%) of pathologic findings, mostly due to the increasing number of advanced stage tumors. high serum levels ... | 1990 | 2191678 |
| a site in the t4 bacteriophage major head protein gene that can promote the inhibition of all translation in escherichia coli. | the cryptic dna element, e14, synthesizes a protein, lit, which can inhibit gene expression late in t4 bacteriophage development. this inhibition is due to the interaction between the lit protein and a short region, the gol region, within gene 23, the major head protein gene of phage t4. we have constructed plasmids in which the gol region is transcribed from the lac promoter and fused translationally and transcriptionally to lacz and cat (chloramphenicol acetyltransferase). these fusion plasmid ... | 1990 | 2191141 |
| novel and sensitive noncompetitive (two-site) enzyme immunoassay for haptens with amino groups. | a novel and sensitive noncompetitive (two-site) enzyme immunoassay for haptens with amino groups is described. l-thyroxine (t4) was used as a model hapten. t4 was indirectly biotinylated with glutathione as spacer between t4 and biotin molecules and trapped onto anti-(t4-bovine serum albumin) igg-coated polystyrene balls. after washing the polystyrene balls to eliminate unreacted biotin and other biotinylated substances, biotinylated t4 was eluted from the polystyrene balls with hcl and was reac ... | 1990 | 2191099 |
| stable albicidin resistance in escherichia coli involves an altered outer-membrane nucleoside uptake system. | albicidin blocked dna synthesis in intact cells of a pola- enda- escherichia coli strain, and in permeabilized cells supplied with all necessary precursor nucleotides, indicating a direct effect on prokaryote dna replication. replication of phages t4 and t7 was also blocked by albicidin in albicidin-sensitive (albs) but not in albicidin-resistant (albr) e. coli host-cells. all stable spontaneous albr mutants of e. coli simultaneously became resistant to phage t6. the locus determining albicidin ... | 1990 | 2191080 |
| ordered deletions for dna sequencing and in vitro mutagenesis by polymerase extension and exonuclease iii gapping of circular templates. | a simple method is described for generating nested deletions from any fixed point in a cloned inset. starting with a single-stranded phagemid template, t4 dna polymerase is used to extend an annealed primer. this leads to a fully double-stranded circular molecule with a nick or small gap just 5' to the primer. exonuclease iii initiates progressive digestion from the resulting 3' end. removal of timed aliquots and digestion with a single-strand specific endonuclease leads to a series of linear ne ... | 1990 | 2190184 |
| thyrotoxicosis due to pituitary resistance to thyroid hormones. successful control with d thyroxine: a study in three patients. | selective pituitary resistance to thyroid hormone (prth) is responsible for thyrotoxicosis due to inappropriate secretion of tsh. the tsh suppressive action of d-thyroxine (dt4) has been previously documented in euthyroid and hypothyroid subjects. this prompted us to treat with dt4 three patients with prth uncontrolled by anti-thyroid drugs (atd) alone or supplemented with bromocriptine, and whose follow-up had been complicated by atrial fibrillation in two patients. because of 100% cross-reacti ... | 1990 | 2189602 |
| [chronic lymphocytic thyroiditis in children and adolescents: apropos of 51 cases]. | fifty one patients were diagnosed as having chronic lymphocytic thyroiditis, according the fisher's criteria. their ages range was between 4.2 to 17.8 years. according to their basal values of t4 and tsh, they were divided into the following groups: 1. euthyroids (n = 14), with both thyroxine and thyrotrophin, within normal limits. 2. hypothyroid group (n = 37), subdivided into: 2.a normal t4 and elevated tsh (n = 18); 2.b low t4 with high tsh (n = 19). the trh test showed an exaggerated tsh res ... | 1990 | 2189331 |
| [transmission of amber mutants in phage t4. v. positive effect of heat shock proteins on the replication of amber mutants in gene 31]. | the effect of growth of escherichia coli be, prior to infection, on multiplication of double amber mutant amn54-amng71 in gene 31, mutant amn131-amng114 in gene 26 and t4d wild-type at different temperatures has been studied. in the case of gene 31 mutant the increase in phage burst size, along with increase in growth temperature, was only observed. and this dependence seems to have the same character as the known dependence of growth temperature on cellular levels of heat shock proteins. possib ... | 1990 | 2188877 |
| organic mental disorders caused by hiv: update on early diagnosis and treatment. | hiv directly affects the cns, primarily causing subcortical neuropathology. dementia as the initial presentation is rare, but organic mental changes that mimic many functional disorders can occur during the course of infection. the mental status examination is not adequately sensitive to detect noncognitive dysfunction, and subjective complaints, neurological signs, reduced t4 lymphocytes, csf abnormalities, diffuse slowing on ecg, mild cerebral atrophy on brain ct, and nonspecific hyperdensitie ... | 1990 | 2188514 |
| [amiodarone and thyroid: the janus effect]. | iodide released from amiodarone during a chronic treatment is 100 x higher than food iodide intake. thyroid adaptation allows normal function but over-adaptation leads to hypothyroidism and hypo-adaptation to thyrotoxicosis. thyrotoxicosis may also result from follicular disruption. amiodarone inhibits 5' deiodinase type i and ii and hence increases serum t4 and (transiently) tsh and decreases serum t3 concentrations. perchlorate and corticoids may be useful in the treatment of thyrotoxicosis. | 1990 | 2187219 |
| folding kinetics of phage t4 thioredoxin. | the folding mechanism for bacteriophage t4 thioredoxin is best described by a four-state box mechanism, n----uc----ut----it----n, where n indicates native, uc the unfolded form with the cis proline isomer, ut unfolded with the trans proline isomer, and it a compact form with a trans proline isomer. both manual mixing fluorescence and size-exclusion chromatography indicate that there is a cis-trans proline isomerization that is important to the folding pathway. furthermore, the data suggest that ... | 1990 | 2186806 |
| dna repair in a small yeast plasmid folded into chromatin. | the question of whether excision repair of yeast plasmids accurately reflects the repair of yeast genomic chromatin has yielded conflicting answers. these conflicts could have arisen from differences in the conformation of plasmid molecules used during these studies. we have examined excision repair of uv photoproducts in a small (2619 bp) autonomously replicating plasmid (yrp-trurap), known to be folded into chromatin with positioned nucleosomes in vivo, in the yeast saccharomyces cerevisiae. a ... | 1990 | 2186374 |
| bacteriophage t4 gene 26. | 1990 | 2186370 | |
| an improved method for the rapid isolation of chromosomal dna from mycoplasma spp. | a method is described for the rapid isolation of chromosomal deoxyribonucleic acid from species of the genus mycoplasma. the method involves incubation of washed cells at elevated temperature in the presence of an ionic detergent, chelating agents, and proteinase k prior to the removal of residual protein and ribonucleic acid with ribonuclease and chloroform. it results in a good yield of high molecular weight material that is shown to be free of endogenous nuclease and substantially free of pro ... | 1990 | 2185871 |
| cis-syn thymine dimers are not absolute blocks to replication by dna polymerase i of escherichia coli in vitro. | both escherichia coli dna polymerase i (pol i) and the large fragment of pol i (klenow) were found to bypass a site-specific cis-syn thymine dimer, in vitro, under standard conditions. a template was constructed by ligating d(pcgtat[c,s]tatgc), synthesized via a cis-syn thymine dimer phosphoramidite building block, to a 12-mer and 19-mer. the site and integrity of the dimer were verified by use of t4 denv endonuclease v. extension of a 15-mer on the dimer-containing template by either pol i or k ... | 1990 | 2185842 |
| an rna polymerase-binding protein that is required for communication between an enhancer and a promoter. | although bacteriophage t4 late promoters are selectively recognized by escherichia coli rna polymerase bearing a single protein encoded by t4 gene 55 (gp55), efficient transcription at these promoters requires enhancement by the three t4 dna polymerase accessory proteins, bound to distal "mobile enhancer" sites. two principles are shown to govern this transcriptional enhancement: (i) promoter recognition and communication between the enhancer and the promoter require separate phage-coded protein ... | 1990 | 2185541 |
| efficacy of adjuvant chemotherapy for patients with resectable head and neck cancer: a subset analysis of the head and neck contracts program. | to evaluate the efficacy of adjuvant chemotherapy for patients with advanced head and neck squamous cancer, the head and neck contracts program conducted a three-arm study comparing standard surgery and radiation, induction chemotherapy (cisplatin and bleomycin) plus standard therapy, and induction chemotherapy plus standard therapy followed by maintenance cisplatin for 6 months. as previously reported, this trial of 462 patients demonstrated no significant difference in disease-free survival or ... | 1990 | 2185340 |
| the dna adenine methyltransferase (dam+) gene of bacteriophage t4 reverses the mutator phenotype of an escherichia coli dam mutant. | the mutator phenotype of escherichia coli dam mutants was found to be reversed by introduction of the bacteriophage t4 gene for dna adenine methyltransferase. this precludes a direct role for the e. coli dna adenine methyltransferase in mismatch repair, in addition to its role in strand discrimination, as suggested by earlier studies (s. l. schlagman, s. hattman, and m. g. marinus, j. bacteriol. 165:896-900, 1986). | 1990 | 2185235 |
| inhibition of transcription of cytosine-containing dna in vitro by the alc gene product of bacteriophage t4. | the alc gene product (gpalc) of bacteriophage t4 inhibits the transcription of cytosine-containing dna in vivo. we examined its effect on transcription in vitro by comparing rna polymerase isolated from escherichia coli infected with either wild-type t4d+ or alc mutants. a 50 to 60% decline in rna polymerase activity, measured on phage t7 dna, was observed by 1 min after infection with either t4d+ or alc mutants; this did not occur when the infecting phage lacked gpalt. in the case of the t4d+ s ... | 1990 | 2185231 |
| promoter elements and regulation of expression of the cysd gene of escherichia coli k-12. | the cysd gene, involved in cysteine biosynthesis in escherichia coli and salmonella typhimurium, is positively regulated by the cysb regulatory protein. the cysd promoter of e. coli k-12 in a 492-bp psti-eco ri fragment was sequenced. the in vivo transcription start point (tsp) for the cysd gene was determined by the methods of t4 dna polymerase mapping and mung-bean nuclease mapping. the -10 region of the cysd promoter (tatagt) is closely homologous to the -10 consensus sequence (tataat) for e. ... | 1990 | 2185135 |
| diagnostic and therapeutic technology assessment. prophylactic treatment for opportunistic infections in hiv-positive patients: aerosolized pentamidine. | the datta panelists considered aerosolized pentamidine to be both safe and effective for primary and secondary prophylaxis of pcp. t4 helper cell counts offer guidance as to the best candidates for primary prophylaxis. patients with a t4 helper cell count of fewer than 200/mm3 are the most appropriate group to receive primary prophylaxis with aerosolized pentamidine. however, t4 helper cell counts are not an exclusive criterion for aerosolized pentamidine prophylaxis. some datta panelists sugges ... | 1990 | 2184263 |
| selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (mad): a vehicle for direct determination of three-dimensional structure. | an expression system has been established for the incorporation of selenomethionine into recombinant proteins produced from plasmids in escherichia coli. replacement of methionine by selenomethionine is demonstrated at the level of 100% for both t4 and e. coli thioredoxins. the natural recombinant proteins and the selenomethionyl variants of both thioredoxins crystallize isomorphously. anomalous scattering factors were deduced from synchrotron x-ray absorption measurements of crystals of the sel ... | 1990 | 2184035 |
| genetic analyses of cellular functions required for uv mutagenesis in escherichia coli. | in escherichia coli, most uv and chemical mutagenesis is not a passive process and requires the participation of the umud and umuc gene products. however, the molecular mechanism of uv mutagenesis is not yet understood and the roles of the umud and umuc proteins have not been elucidated. the umudc operon is induced by uv irradiation and regulated as part of the sos response. genetic evidence now indicates that reca-mediated cleavage activates umud for its role in mutagenesis. the cooh-terminal f ... | 1990 | 2183772 |
| responses of four tooth and site groupings to periodontal therapy. | the responses of four tooth/site groupings to periodontal therapy were evaluated. eighty-two patients with periodontitis were treated in a split mouth design with coronal scaling, root planing, modified widman surgery, and flap with osseous resectional surgery. patients were evaluated prior to therapy, 4 weeks post-phase i therapy, 10 weeks post-phase ii therapy, and at yearly intervals during 2 years of maintenance therapy. the tooth/site groupings evaluated were: 1) interproximal sites of sing ... | 1990 | 2181110 |
| virology, immunology, and clinical course of hiv infection. | human immunodeficiency virus (hiv), the cause of aids, was the second retrovirus to be associated with human diseases. the unique life cycle of retroviruses involves reverse transcription of their rna to dna, which is incorporated into their host's genes. hiv, despite its small genome, contains multiple structural and regulatory genes, the latter controlling the rate of viral replication. experimental vaccines are aimed at inhibiting binding of hiv to host cells. azidothymidine (azt) and other n ... | 1990 | 2181004 |
| transcriptional mapping of a dna replication gene cluster in bacteriophage t4. sites for initiation, termination, and mrna processing. | a phage t4 genetic cluster that encodes dna polymerase, several other dna replication proteins, transcriptional factors, and the translation repressor rega has been shown to be controlled by overlapping modes of transcription which initiate at several promoters. the promoters were mapped by using a combination of assays including northern blotting, s1-mapping, rna sequencing, and analysis of products of radioactive labeling of 5' ends on t4-induced rna in vitro via the reaction catalyzed by euka ... | 1990 | 2180963 |
| hormone and dna binding activity of a purified human thyroid hormone nuclear receptor expressed in escherichia coli. | using a t7 expression system, large amounts of the human placental c-erba protein (h-tr beta 1) were expressed. from 1 liter of escherichia coli culture, approximately 50-100 micrograms of purified h-tr beta 1 were obtained. analysis of the binding data indicated that the purified h-tr beta 1 binds to 3,3',5-triiodo-l-thyronine (t3) with a ka = 2.8 x 10(9) m-1. it binds to 3,3',5-triiodo-l-thyropropionic acid, 3,3',5-triiodo-l-thyroacetic acid, d-t3, l-thyroxine (t4), and 3',5',3-triiodo-l-thyro ... | 1990 | 2180960 |
| selective affinity labeling of a 27-kda integral membrane protein in rat liver and kidney with n-bromoacetyl derivatives of l-thyroxine and 3,5,3'-triiodo-l-thyronine. | 125i-labeled n-bromoacetyl derivatives of l-thyroxine and l-triiodothyronine were used as alkylating affinity labels to identify rat liver and kidney microsomal membrane proteins which specifically bind thyroid hormones. affinity label incorporation was analyzed by ethanol precipitation and individual affinity labeled proteins were identified by autoradiography after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. six to eight membrane proteins ... | 1990 | 2180943 |
| measurement of free hormones in blood. | the binding by serum proteins of circulating thyroid and steroid hormones is a phenomenon whose physiological significance is still not understood, and the validity of the free hormone hypothesis remains in doubt. indeed, even the most basic physicochemical consequences of these proteins' presence within the microcirculation continue to generate controversy, reflecting disagreement of the rate-limiting effects of hormone-protein interactions on hormone efflux from protein-containing fluid compar ... | 1990 | 2180687 |
| [thyroid diseases in the elderly]. | thyroid dysfunction in the elderly differs from that in the young, because most symptoms at presentation are absent, or non specific or atypical. hypothyroid patients generally present with cardiovascular or central nervous system symptoms. a serious complication, occurring more easily in elderly is hypothyroid coma. the diagnosis of hypothyroidism is confirmed by a high serum tsh and low values of ft4 and t4. elderly patients should be started on low doses of thyroid-hormone replacement that ar ... | 1990 | 2180141 |
| a new method for the synthesis of a structural gene. | a novel method of synthesizing a structural gene or gene fragment, consisting of the first synthesis of a single-stranded dna (ssdna), has been developed. as a preliminary test of this method, four synthetic genes or gene fragments have been synthesized. the first one with 396 base pairs (b.p.) codes for the mature rbcs from wheat, the next two with 370 and 342 b.p. respectively, for two half molecules of a gene for trichosanthin and the last one with 315 b.p. for the n-terminal 1-102 residues o ... | 1990 | 2179872 |
| acute endotoxin-induced lymphocyte subset sequestration in sheep lungs. | endotoxemia is associated with an early phase of pulmonary hypertension and a later increase in microvascular permeability. these physiologic changes are attended by peripheral blood and lung lymph leukopenia and a rapid accumulation of both granulocytes and lymphocytes in the peripheral lung. in the present study, the numbers of lymphocytes in blood, lung lymph, and lung tissue after infusion of endotoxin were determined by fluorescent labeling of lymphocyte populations with monoclonal antibodi ... | 1990 | 2179624 |
| immunohistologic study of mononuclear cell infiltrates in oral squamous cell carcinomas. | intensity of mononuclear cell infiltrates in 50 oral cancer tissues was evaluated by immunohistochemical stainings. the intensity of the infiltrates was graded into four degrees from marked ( ) to absent (-); and the degree of each infiltrated lymphocyte subpopulation including leu-3a3b/leu-2a ratio was matched against clinical and histopathologic features. the vast majority of the infiltrated lymphocytes had antigens which were reactive with anti-t cell antibodies. in t cell subsets, leu-3a3b+ ... | 1990 | 2179166 |
| susceptibility to hiv-1 infection of a human b-lymphoblastoid cell line, dg75, transfected with subgenomic dna fragments of epstein-barr virus. | we have previously shown that the presence of the ebv genome and active ebv infection in some burkitt's lymphoma cell lines confer the susceptibility of hiv-1 infection in spite of the absence of surface cd4 receptors in these cells. in the present study we used an ebv genome negative burkitt's lymphoma b-cell line, dg75, transfected with three different subgenomic fragments of ebv expressing the nuclear antigens (ebna1 and ebna2) and the latent membrane protein (lmp), respectively. immunofluore ... | 1990 | 2178126 |
| [effects of thyroxine on igm production and beta-adrenergic receptor on lymphocyte in mice]. | the effects of thyroxine (l-t4) on the igm production of splenocytes in mice induced by sheep red blood cell (srbc) were investigated by means of spectrophotometry of quantitative hemolysis (qhs). the maximum binding site number (bmax) and affinity (kd) of beta-adrenergic receptors (beta-adr. r.) on splenic lymphocytes of mice under the different thyroid functional status during immune response were determined by radio-ligand combination method. the igm production and bmax of beta- adr. r. of sp ... | 1990 | 2177571 |
| effects of long-term, low-dose, time-specified melatonin administration on endocrine and cardiovascular variables in adult men. | six healthy adult male volunteers underwent serial blood drawings at 4-hour intervals over 24 hours for the definition of melatonin (mt), prolactin (prl), cortisol, and testosterone circadian patterns. serum levels of triiodotironine (t3) and thyroxine (t4) were determined at 0800. systolic and diastolic blood pressure and heart rate were automatically recorded every 30 minutes for 24 hours. the responses of luteinizing hormone (lh), follicle stimulating hormone (fsh), prl, thyroid stimulating h ... | 1990 | 2177501 |
| high ionic strength promotes selective 3'-phosphatase activity of t4 polynucleotide kinase. | 1990 | 2176507 | |
| [a case of myxedema heart showing the improvement of impaired alpha-hanp secretion by administration of atp and dibutyryl camp]. | a 40-year old female was admitted with complaints of general fatigue and dyspnoea brought on by effort. there were edema on the face, a diffuse and slightly hard goiter on the neck and non-pitting edema in the lower legs. laboratory findings showed low levels of serum t3 (0.37 ng/ml) and t4 (2.0 micrograms/dl), a very high level of serum tsh (549.8 microu/l), positive thyroid test (x 400) and positive microsome test (x 102,400). the chest roentgenogram showed an enlargement (ctr 62%) of the card ... | 1990 | 2176340 |
| studies on the initiation and elongation reactions in the simian virus 40 dna replication system. | the synthesis of oligoribonucleotides by dna primase in the presence of duplex dna containing the simian virus 40 (sv40) origin of replication was examined. small rna chains (10-15 nucleotides) were synthesized in the presence of the four common ribonucleoside triphosphates, sv40 large tumor antigen (t antigen), the human dna polymerase alpha (pol alpha)-dna primase complex, the human single-stranded dna-binding protein (hssb), and topoisomerase i isolated from hela cells. the dna primase-cataly ... | 1990 | 2175912 |
| excision repair of uv-damaged plasmid dna in xenopus oocytes is mediated by dna polymerase alpha (and/or delta). | we studied dna repair by injecting plasmids containing random pyrimidine dimers into xenopus oocytes. we demonstrated excision repair by recovering plasmids and analyzing them with t4 uv endonuclease treatment and alkaline agarose gel electrophoresis. the mechanism for excision repair of these plasmids appears to be processive, rather than distributive, since repair occurs in 'all or none' fashion. at less than 4-5 dimers/plasmid, nearly all repair occurs within 4-6 hours (approximately 10(10) d ... | 1990 | 2175436 |
| [plasma levels of cyclic nucleotides, phosphodiesterase, thyroid hormones and insulin in patients with ischemic stroke]. | the content of cyclic nucleotides (camp and cgmp), hormones (t3, t4, insulin, protein-bound iodine and thyroid-stimulating hormone) and phosphodiesterase activity were examined in the acute period and over time in blood plasma of patients with ischemic stroke. the parameters under study were found to be interrelated. also, it has been established that the t4/t3 and camp/cgmp ratios and the content of insulin may serve as important biochemical criteria for the gravity of ischemic stroke. | 1990 | 2175082 |
| intratumor injection of ok-432 for the treatment of small hepatocellular carcinoma. | intratumor injection of ok-432, a biological response modifier, in the treatment of small hcc was studied in 7 inoperable patients. after evaluation with ultrasound (us), computed tomography (ct), angiography and us-guided biopsy, implantation of a steel coil in the tumor, intratumor injection was performed under us guidance. after completion of the treatment, liver biopsy and image studies were again done to evaluate the extent of tumor necrosis. one patient was alive and well without recurrenc ... | 1990 | 2174823 |
| positive and negative transcriptional elements of the human type iv collagenase gene. | proteolysis by type iv collagenase (t4) has been implicated in the process of tumor metastasis. the t4 gene is expressed in fibroblasts, but not in normal epithelial cells, and its expression is specifically repressed by the e1a oncogene of adenovirus. we present an investigation of the transcriptional elements responsible for basal, e1a-repressible, and tissue-specific expression. 5'-deletion analysis, dnase i footprinting, and gel mobility shift assays revealed a strong, e1a-repressible enhanc ... | 1990 | 2174109 |
| evidence for a common mechanism of action for antitumor and antibacterial agents that inhibit type ii dna topoisomerases. | numerous antitumor and antibacterial agents inhibit type ii dna topoisomerases, yielding, in each case, a complex of enzyme covalently bound to cleaved dna. we are investigating the mechanism of inhibitor action by using the type ii dna topoisomerase of bacteriophage t4 as a model. the t4 topoisomerase is the target of antitumor agent 4'-(9-acridinylamino)-methanesulfon-m-anisidide (m-amsa) in t4-infected escherichia coli. two m-amsa-resistant phage strains were previously isolated, one with a p ... | 1990 | 2173709 |
| induction of thyroid hormone in changing na+k(+)-atpase activity in different organs of toad, bufo melanostictus. | single injection of t3, at the doses of 0.5 and 1 micrograms/g body weight, stimulated na+k(+)-atpase activity of crude liver homogenate of toad in a dose dependent fashion. lowering the dose of t3 to 0.25 micrograms/g had no effect on the enzyme activity. t3 at the dose of 2 micrograms/g showed the same level of enzyme activity at par with that of 1 microgram/g. na+k(+)-atpase activity of muscle increased with t3 at the doses of 1 and 2 micrograms/g, but without any dose dependent manner while ... | 1990 | 2173671 |
| [relations between sheehan's syndrome and empty sella turcica. a functional study apropos of 6 cases]. | we evaluated six patients in whom a diagnosis of sheehan's syndrome had been made. the plasma levels of the following hormones were measured: basal thyroxine (t4), estradiol and cortisol; and also follicle-stimulating hormone (fsh), luteinizing hormone (lh), growth hormone (gh), thyrotropin (tsh), prolactin (prl) and adrenocorticotropic hormone (acth), basally and after acute challenge with lh releasing hormone (lhrh), grf (1-29)nh2 or insulin hypoglycemia, tsh releasing hormone (trh) and lysine ... | 1990 | 2172669 |
| quantitative analysis of spinally projecting adrenaline-synthesising neurons of c1, c2 and c3 groups in rat medulla oblongata. | spinal projections of the phenylethanolamine n-methyltransferase (pnmt) immunoreactive neurons of the medulla were investigated using a combination of immunohistochemistry and retrograde transport of colloidal gold particles conjugated to cholera toxin b subunit (ctb-gold). the pnmt-containing adrenergic neurons were localised in three groups, the c1 group in the rostral ventrolateral medulla, the c2 group in the nucleus tractus solitarius/dorsal vagal motor complex in the dorsal medulla and the ... | 1990 | 2172354 |
| transthyretin receptors on human astrocytoma cells. | transthyretin (ttr), a transport protein for t4 and retinol-binding protein, is the principal t4-binding protein of cerebrospinal fluid. its function in regard to the delivery of its ligands and in other respects is unclear. the binding of [125i] ttr to cultured human astrocytoma cells was studied to determine whether these cells carry receptors for ttr. scatchard analysis was consistent with a single class of binding sites with a kd of 3 nm. no significant cross-reactivity with transferrin or s ... | 1990 | 2172276 |
| increase of igf i binding to erythrocyte receptors during the trh-test: correlation between igf i binding and thyroid hormone values. | the effects of trh on insulin-like growth factor i receptors were investigated on erythrocytes from 7 gh-deficient children having plasma gh levels less than 10 ng/ml during two provocation tests. intravenous injection of synthetic trh (0.2 mg/m2) was followed by a marked increase of igf i binding on erythrocytes, from 3.9% +/- 0.3% to 5.9% +/- 0.3% (p less than 0.005) after 1 hour and 7.3% +/- 0.4% (p less than 0.005) after 2 hours. the igf i binding variations were due to an increase in both t ... | 1990 | 2172134 |
| [evaluation of adult idiopathic growth hormone deficiency with other pituitary hormones deficiency]. | the type and percentage of multiple pituitary hormone deficiency (mphd) were studied in 42 patients with idiopathic growth hormone deficiency (ighd). it was found that the development of secondary sexual characteristics was poor or absent in 39 patients (93%) with gonadotropin deficiency (gnd). mean serum testosterone (t), luteinizing hormone (lh) and follicle-stimulating hormone (fsh) levels in the 39 patients were significantly less than those of normal adult males (p less than 0.01). mean tes ... | 1990 | 2171885 |
| neuroendocrinological investigations during sleep deprivation in depression. ii. longitudinal measurement of thyrotropin, th, cortisol, prolactin, gh, and lh during sleep and sleep deprivation. | thyrotropin (tsh), thyroxin (t4), triiodothyronine (t3), free t3 (ft3), cortisol, prolactin, and human growth hormone (hgh) were measured every 2 hr during a night of sleep, the following day, and a night of sleep deprivation (sd) in 14 patients with major depressive disorder. in subgroups ft4 (n = 5), reverse t3 (rt3), and luteinizing hormone (lh) (n = 6) were also investigated. significant increases in tsh, t4, ft4, t3, ft3, rt3, and cortisol and decreases in prolactin levels occurred during t ... | 1990 | 2171684 |
| unique structural properties of apolipoprotein b in low-density lipoproteins produced by several human hepatoma-derived cell lines. | previous work has shown that low-density lipoproteins (ldl) secreted by hepatoma-derived cell lines have an unusual composition compared to plasma ldl; rather than cholesteryl ester, the hepatoma cell-secreted ldl have a triacylglycerol core. we have found that they also have an increased negative charge, as judged by agarose electrophoresis. since apolipoprotein b is a glycoprotein containing carbohydrate chains terminated with negatively charged sialic acid residues, we examined whether increa ... | 1990 | 2171671 |
| alpha 1- and beta-adrenergic receptors in brown adipose tissue and the adrenergic regulation of thyroxine 5'-deiodinase. | the density of alpha 1- and beta-adrenergic receptors and the adrenergic regulation of the type ii thyroxine 5'-deiodinase were investigated in brown adipose tissue. the density of the alpha 1- and beta-adrenergic receptors was determined in membranes of brown fat from animals in different physiological states, i.e. in cold-acclimated, neonatal, cafeteria-fed, genetically obese and hypothyroid animals. the density of the alpha 1-adrenergic receptors in brown fat correlated well with the recruitm ... | 1990 | 2171299 |
| [endosonographic staging of rectal tumors]. | pre-operative staging was performed in 81 patients with rectal tumours by means of endorectal sonography. in 87% of cases (70 out of 81), the endosonographic findings corresponded with the histopathological appearance; the recognition of t0 and 1 tumours, which is important in deciding surgical procedures, was possible in 94% (51 out of 54). differentiation between t0 and t1 tumours (i.e. between adenomas and carcinomas infiltrating the submucosa) was not possible. five out of ten of t2 and 14 o ... | 1990 | 2171080 |
| factors determining the differential tissue response of na/k-atpase to thyroid hormone in the neonatal rat. | the concentration of ouabain binding sites (obs), a measure of the abundance of sodium/potassium-dependent atpase (na/k-atpase), was measured in cerebral cortex (cc), cerebellum (cbl), brown adipose tissue (bat), and heart of neonatal rats with congenital hypothyroidism. in euthyroid rats, the concentration of obs was 50- to 100-fold greater in the nervous tissue than in the nonnervous tissue. congenital hypothyroidism resulted in a significant reduction in the number of obs in all four tissues. ... | 1990 | 2170819 |
| characterization of a bimobile dna junction. | we present here a chemical and enzymatic footprinting analysis of a branched dna molecule formed from four complementary 50-mer strands. these strands are designed to form a stable junction, in which two steps of branch point migration freedom are possible. exposure of the junction to fe(ii).edta shows protection of 3 or 4 residues in each strand at the branch, while two resolvase enzymes (endonuclease vii from phage t4 and endonuclease i from phage t7), cleave all four strand near the branch. c ... | 1990 | 2170355 |
| distinct transcriptional effects of camp on 2 thyroid specific genes: thyroperoxidase and thyroglobulin. | the production of the thyroid hormones by the thyroid tissue is regulated by thyrotropin (tsh). tsh, through camp, enhances all steps of t3 and t4 synthesis, among which transcription of the genes encoding the precursor protein, thyroglobulin (tg) and the enzyme responsible for the iodination and coupling mechanisms, thyroperoxidase (tpo). run-on transcription assays show that the kinetics of tg gene transcriptional activation by camp is slow (8 to 16 hours) in dog thyrocytes in primary culture, ... | 1990 | 2170261 |
| effect of hypophysectomy on caffeine elimination in rats. | two groups of 8-week-old sprague-dawley male rats were used: 8 hypophysectomized (h[-]), operated on day 0, treated by daily sc tetracosactid (acths: 10 micrograms), and thyroxine (t4:5 micrograms/100 g); 7 sham-operated, treated by sc saline solution. acths, t4, saline solution were administered on days 7-16. the animals received po caffeine (caf) 4 mg/kg as citrate salt on day 15. ten blood samples were drawn from the tail. plasma caf concentrations were determined by hplc. caf apparent cleara ... | 1990 | 2170254 |
| electron microscopic analysis of dna forks generated by escherichia coli dna helicase ii. | t7 phage dna eroded with lambda exonuclease (to create 3'-protruding strands) or exonuclease iii (to create 5'-protruding strands) was treated under unwinding assay conditions with dna helicase ii. single-stranded dna-binding protein (of escherichia coli or phage t4) was added to disentangle the denatured dna and the complexes were examined in the electron microscope. dna helicase ii complexes filtered through a gel column before assay retain the ability to generate forks suggesting that dna hel ... | 1990 | 2170129 |
| mitotic rate, dna distribution, and chromatin in situ sensitivity to heparin in breast cancer. | the purpose of this study was to characterize breast carcinomas by cell kinetic parameters. mitotic rate (mr) and flow cytometrically (fcm) measured cell cycle distribution as well as chromatin testing in situ employing heparin for determination of activated chromatin, provided the following results: mr counted in 73 unselected carcinomas showed an increase up to a tumor size of 4.2 cm (p less than 0.05); beyond this diameter, the mr was found to decrease. in t1-t2 carcinomas, cell cycle stage a ... | 1990 | 2169927 |
| 1,25-dihydroxyvitamin d3 receptors in rat lung during the perinatal period: regulation and immunohistochemical localization. | we have previously reported that in contrast to what has been described in adult lung, 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] has specific binding sites in rat fetal lung at the end of gestation, and it stimulates in vitro the phospholipid biosynthesis and surfactant release from fetal rat type ii pneumocytes. in the present study an immunohistochemical technique using a rat monoclonal antibody (9a7 gamma) and binding studies were carried out on fresh lung tissues from fetal and newborn rats du ... | 1990 | 2169401 |
| [changes in thyroid function during acth therapy in patients with infantile spasms]. | serum triiodothyronine (t3), free t3, thyroxine (t4) and free t4 levels were measured before and immediately after daily acth-z therapy (0.01 mg/kg/day, 1-2 weeks) for patients with 9 cases of infantile spasms and one case of myoclonus epilepsy. in some of them, serum reverse t3 (rt3) and thyroxine binding globulin (tbg) levels were also measured. all patients became seizure free after acth-z therapy. before acth-z therapy, all of the hormone levels were within normal limits. serum t3 and free t ... | 1990 | 2169274 |
| two ultraviolet tumor-specific suppressor cell clones. one expresses ig rna and the other expresses t cell receptor-alpha and -beta rna. | two splenic cell clones were derived from mice bearing uv-induced skin tumors; both clones are i-j+, thy-1+, and j11d+. cellular extracts, but not culture supernatants, from the two clones, designated t4 and t5, specifically suppress primary cytotoxic and proliferative responses to uv-induced tumor targets. therefore, clones t4 and t5 would appear to function as putative i-j+ suppressor cells with specificity for an antigenic epitope common to several uv-induced tumors. to derive more informatio ... | 1990 | 2168916 |
| [decompensation of physiologic hypocalcemia in infants inducing vitamin d deficiency rickets]. | 43 infants taking the same dosage of vitamin d and calcium were investigated. the results showed that vitamin d deficiency rickets (vdr) were still present in some infants when vitamin d was sufficient, because of deficiency of 1,25-dihydroxyvitamin d (1,25-(oh)2d3). serum calcium, phosphorus, alkaline phosphatase, triiodothyronine (t3), thyroxine (t4), calcitonin (ct), immunoreactive parathyroid hormone (ipth), and 25-hydroxyvitamin d (25 ohd3) concentrations were compared among infants with no ... | 1990 | 2168795 |
| antithyroid peroxidase autoantibodies in thyroid diseases. | thyroid microsomal antibodies (anti-m ab) have been recently proven to be directed to thyroid peroxidase (tpo). methods to detect anti-tpo antibodies (anti-tpo ab) employing purified antigen have been developed, but the available information on the clinical usefulness of this technique is still limited to small patient series. in the present investigation anti-tpo ab were assayed by a newly developed monoclonal antibody-assisted ria in a large number (n = 715) of subjects, including 119 normal c ... | 1990 | 2168432 |
| "macromolecular crowding": thermodynamic consequences for protein-protein interactions within the t4 dna replication complex. | in vitro biochemical assays are typically performed using very dilute solutions of macromolecular components. on the other hand, total intracellular concentrations of macromolecular solutes are very high, resulting in an in vivo environment that is significantly "volume-occupied." in vitro studies with the dna replication proteins of bacteriophage t4 have revealed anomalously weak binding of t4 gene 45 protein to the rest of the replication complex. we have used inert macromolecular solutes to m ... | 1990 | 2168402 |
| sequence analysis of conserved rega and variable orf43.1 genes in t4-like bacteriophages. | bacteriophage t4 rega protein is a translational repressor of several phage mrnas. in the t4-related phages examined, rega nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. the exceptional phage, rb69, did not produce a rega protein reproducibly identifiable by western blots (immunoblots) nor did it produce mrna that hybridized to t4 rega primers. nucleotide sequences of either 223 or 250 base pairs were identified immediately 3' to rega in rb18 and r ... | 1990 | 2168375 |
| thyrotropin-and prolactin--secreting pituitary tumor dissociated hormonal response to bromocriptine. | a 41-year-old male with recurrent hyperthyroidism resulting from pituitary tumor was studied. because of recurrent hyperthyroidism, he had been treated with propylthiouracil for four years and had been operated. at the time of high serum thyroid hormone (t4: 20 micrograms/dl; t3:502 ng/dl), there was also elevated basal serum tsh level (55 microu/ml) which could not be suppressed by exogenous thyroid hormone administration. in addition, the serum tsh showed blunt response to intravenous trh infu ... | 1990 | 2168264 |
| [in vitro and in vivo effect of thyroid hormones on the growth of neuroblastoma cells. ii. the effect of thyroxine in vivo]. | we studied the effect of thyroxine (t4 0.050 mg/kg/d, i.p.), tsh (0.08 u/kg/d, i.p.) and hypothalamic peptide (hf; 1 mg protein/kg/d, i.p.) given alone or in combination, on the growth of murine (nb c-1300) and human (nb park) neuroblastoma transplanted onto the nude mouse (nu/nu). both t4 and tsh caused a significant increase (perchlorate a decrease) of the serum t3. histologically, the t4 treatment was followed by partial tumor necrosis and a marked growth of connective tissue within the tumor ... | 1990 | 2168040 |
| regulation of sex hormone-binding globulin production by growth factors. | sex hormone-binding globulin (shbg) is a glycoprotein whose production has been demonstrated to be regulated by both sex steroids, as well as by thyroid hormone and peptide hormones such as insulin. however, none of these regulatory factors would explain the marked decrease in serum shbg seen throughout the prepubertal and pubertal time period in both boys and girls. furthermore, current in vitro data show that both androgens and estrogens can stimulate shbg production by the human hepatoblastom ... | 1990 | 2168012 |
| stimulation of oxidative metabolism by thyroid hormones in an apodan amphibian, gegenophis carnosus (beddome). | administration of different doses of l-thyroxine (t4) and triiodo-l-thyronine (t3) in vivo in g. carnosus stimulated the activities of cytochrome oxidase, alpha-glycerophosphate dehydrogenase (alpha-gpdh), succinate dehydrogenase (sdh), and mg2+ adenosine triphosphatase (mg2+ atpase) and inhibited the activity of malate dehydrogenase (mdh). while a low dose of thiouracil administration produced a stimulatory effect on cytochrome oxidase and alpha-gpdh activities, a higher dose of thiouracil sign ... | 1990 | 2167865 |
| [experimental study of thyroid hormone and bone metabolism in the rat]. | thyroid hormones (t3, t4) stimulate bone turnover. calcitonin is a potent inhibitor of osteoporostic bone resorption. in patients with thyrotoxicosis, bone mineral content is reduced. we investigated the influence of calcitonin deficiency in thyroidectomized parathyroid-autotransplanted rats with l-thyroxine (t4) supplementation. thyroidectomy resulted in a reduction of bone volume, bone calcium content and in a decrease in trabecular bone volume. t4-supplementation corrected this loss of bone c ... | 1990 | 2167757 |
| [endocrine abnormalities in hiv infections]. | the finding of endocrine gland lesions at pathological examination in aids and reports of several cases of endocrine disease in patients with this syndrome have prompted us to study endocrine functions in 63 patients (51 men, 12 women) with hiv-1 infection. according to the center for disease control (cdc) classification system, 13 of these patients were stage cdc ii, 27 stage cdc iii and 23 stage cdc iv. we explored the adrenocortical function (acth, immediate tetracosactrin test) and the thyro ... | 1990 | 2167475 |
| role of cytochrome p450 in the control of the production of erythropoietin. | effects of agents affecting cytochrome p450 were studied on the production of erythropoietin (epo) in cultures of the human hepatoma cell line hepg2. epo was measured by radioimmunoassay of the culture media after 24 h of incubation. the addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome p450, significantly enhanced the formation of epo. likewise, the thyroid hormones t3 and t4 stimulated the rate of the production of epo. on the other hand, the formation of epo was lower ... | 1990 | 2167416 |
| synergistic effect of thyroid hormone and thyrotropin on iodothyronine 5'-deiodinase in frtl-5 rat thyroid cells. | the effects of t3 and t4 on the iodothyronine 5'-deiodinase (5'-d) activity in frtl-5 rat thyroid cells were investigated. t3 and t4 stimulated the 5'-d activity in a dose-dependent manner. kinetic studies showed that the stimulation of the 5'-d by t3 was associated with an increase in maximum velocity (vmax) in [11.9 +/- 0.2 (mean +/- se) and 25.4 +/- 0.9 pmol i-released/mg protein.min, respectively, in control and cultured with 10(-9) m t3 for four days] but without a change in apparent michae ... | 1990 | 2167209 |
| related domains in yeast trna ligase, bacteriophage t4 polynucleotide kinase and rna ligase, and mammalian myelin 2',3'-cyclic nucleotide phosphohydrolase revealed by amino acid sequence comparison. | related domains containing the purine ntp-binding sequence pattern have been revealed in two enzymes involved in trna processing, yeast trna ligase and phage t4 polynucleotide kinase, and in one of the major proteins of mammalian nerve myelin sheath, 2',3'-cyclic nucleotide 3'-phosphohydrolase (cnpase). it is suggested that, similarly to the trna processing enzymes, cnpase possesses polynucleotide kinase activity, in addition to the phosphohydrolase one. it is speculated that cnpase may be an au ... | 1990 | 2166684 |
| thyroxine induces transition of red towards white muscle in cultured heart cells. | thyroid hormones (th) have previously been shown to alter the force and velocity of cardiac muscle contractions. to investigate the mechanism responsible for these alterations, excess amounts of thyroxine (t4, 1 microm) were applied on rat heart cells grown in cell culture. we found the following biochemical alterations: a) 40% decrease in the myoglobin content within 2 days; b) 25% increase in the rate of ca-uptake into sacroplasmic reticulum (sr) in myocytes following chemical skinning; and c) ... | 1990 | 2166497 |
| na(+)-k+ pump in rat muscle: effects of hypophysectomy, growth hormone, and thyroid hormone. | we investigated the effects of growth hormone and thyroid hormone on the synthesis of na(+)-k+ pumps in rat soleus muscle. hypophysectomized rats were treated for 11 days with saline, human growth hormone (hgh; 140 micrograms/day), thyroxine (t4; 3 micrograms/day), or hgh plus t4. age-matched nonhypophysectomized control rats received no treatment. the concentration of na(+)-k+ pumps was reduced by 75% in the hypophysectomized rats. treatment with hgh alone or combined with t4 restored the growt ... | 1990 | 2166438 |
| postoperative radiation therapy for major salivary gland malignancies. | between 1966 and 1982 there have been 46 patients treated with surgery plus post-operative radiation therapy for malignant tumors of salivary gland origin. the indication(s) for radiotherapy included positive margins (42%), advanced local tumor (37%), positive nodes (33%), or high grade histology (48%). overall actuarial local control at 5 years was 73%, being 100% for t1, 83% for t2, 80% for t3, and 43% for t4. actuarial survival at 5 years was 80% for t1, 83% for t2, 60% for t3, and 48% for t4 ... | 1990 | 2166187 |
| hormonal responses to cold exposure in subjects with vibration syndrome. | responses of the autonomic nervous system and the endocrine system to cold exposure were investigated. the subjects were all males, between 41 and 69 years of age. seventy subjects with the vibration syndrome and vibration-induced white finger (vwf(+) group), 70 subjects with the vibration syndrome without vwf (vwf(-) group) and 70 healthy subjects (control group) were individually matched according to age. the subjects remained in a cold room at 7 c for 30 minutes. catecholamines, cyclic nucleo ... | 1990 | 2166181 |
| hypothalamic-pituitary and thyroid function in chronic alcoholics with neurological complications. | endocrinological tests were performed in 14 chronic alcoholic men with signs of intellectual impairment and/or peripheral neuropathy. all had been abstinent from alcohol for at least 1 month. basal serum growth hormone (gh) was consistently increased in only one patient whereas the gh responses to insulin hypoglycemia stimulation was normal in all patients. thyroid function values (t4, t3, rt3, tsh) were normal in all patients whereas baseline serum prolactin values were significantly increased ... | 1990 | 2165749 |
| intron mobility in phage t4 is dependent upon a distinctive class of endonucleases and independent of dna sequences encoding the intron core: mechanistic and evolutionary implications. | although mobility of the phylogenetically widespread group i introns appears to be mechanistically similar, the phage t4 intron-encoded endonucleases that promote mobility of the td and suny introns are different from their eukaryotic counterparts. most notably, they cleave at a distance from the intron insertion sites. the td enzyme was shown to cleave 23-26 nt 5' and the suny endonuclease 13-15 nt 3' to the intron insertion site to generate 3-nt or 2-nt 3'-oh extensions, respectively. the abso ... | 1990 | 2165250 |
| comparison of interleukin 2 and gamma-interferon production by peripheral blood mononuclear cells in response to cytomegalovirus after marrow transplantation. | forty-five patients were prospectively studied during the first 100 days after allogeneic marrow transplant to determine the relationship between cytomegalovirus-specific gamma-interferon and interleukin 2 production by peripheral blood mononuclear cells and cmv infection. il-2 production was significantly lower than normal throughout the study period (p = 0.0001). in contrast, mitogen-induced gamma-interferon production was in the normal range by days 41-60 while cmv antigen induced gamma-inter ... | 1990 | 2164265 |
| [immunocytochemical characterization of malignant pleural effusions of small cell bronchial cancer]. | in pleural effusions, histologically proven to be malignant in 13 patients with small cell lung cancer, the immunocytological profile of tumour cells, together with the distribution of the lymphocyte subpopulations, were established. in all cases, tumor cells were demonstrable with the monoclonal antibodies ema, hea-125, sam 2, 6, 8 and 10. in contrast, conventional cytology was positive in only 6 of the 13 cases. as in other malignant or inflammatory effusions, phenotyping of the lymphocytes re ... | 1990 | 2164207 |
| a nascent peptide is required for ribosomal bypass of the coding gap in bacteriophage t4 gene 60. | bacteriophage t4 dna topoisomerase gene 60 contains a 50 nucleotide untranslated region within the coding sequence of its mrna. translational bypass of this sequence by elongating ribosomes has been postulated for the mode of synthesis of an 18 kd polypeptide specified by the split coding segments. ribosome bypass of the untranslated region also occurs when a segment of gene 60 is fused to lacz and expressed in e. coli. the efficiency of bypass in these gene 60-lacz fusions approaches 100%. here ... | 1990 | 2163764 |
| [abnormal hormone values: do drugs play a role? abnormal prolactin, tsh and thyroid hormone values]. | whenever abnormal hormone values are found in a patient, it is necessary to inquire whether medicaments may be implicated. some of the mechanisms of drug induced hormonal alterations are presented, and drugs which affect and alter prolactin, tsh, thyroid hormones and (in brief) gh and acth are summarized and discussed for the non-specialist. hyperprolactinemia is caused by estrogens, neuroleptics and other dopaminantagonists, antidepressants, opioids, reserpine, a-methyl-dopa, h2-receptor blocke ... | 1990 | 2163099 |
| superfamily of uvra-related ntp-binding proteins. implications for rational classification of recombination/repair systems. | a superfamily of proteins encoded by bacterial, phage and eukaryotic genomes and performing a wide range of ntp-dependent functions was delineated by amino acid sequence comparison. the new superfamily brought together bacterial proteins uvra, recf, recn, muth and hexa, t4 phage gp46, t5 phage d13 protein, lambda phage ea59 protein and yeast rad50 protein, all involved in recombination, repair and, in some cases, also in replication of respective genomes, and a family of bacterial and eukaryotic ... | 1990 | 2162963 |
| mcri: a novel class-ii restriction endonuclease from micrococcus cryophilus recognizing 5'-cgry/cg-3'. | a new class-ii restriction endonuclease, mcri, with a novel sequence specificity as isolated from the gram-positive eubacterium micrococcus cryophilus. mcri recognizes the palindromic hexanucleotide sequence. [sequence: see text] the novel enzyme in the presence of mg2(+)-ions cleaves specifically both strands as indicated by the arrows. the staggered cuts generate 3'-protruding ends with single-stranded 5'-ry-3' dinucleotide extensions. the mcri recognition sequence was deduced from mapping dat ... | 1990 | 2162784 |
| single-step labeling of dna using restriction endonucleases and t4 polynucleotide kinase. | dna restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus t4 polynucleotide kinase, omitting a dephosphorylation step. both pbr322 and phage lambda dna digests are efficiently labeled in one step, one tube and one incubation. | 1990 | 2162682 |
| interaction of a four-way junction in dna with t4 endonuclease vii. | the binding of a synthetic four-way junction in dna by t4 endonuclease vii has been studied using gel retardation and footprint analysis. two specific protein-dna complexes have been observed, but only one is stable in the presence of moderate concentrations of salt. the footprint of t4 endonuclease vii in the salt-resistant complex has been probed using hydroxyl radicals generated by the reaction of iron(ii)/edta with hydrogen peroxide. the hydroxyl radical cleavage pattern indicates protection ... | 1990 | 2160971 |
| bacteriophage t4 deoxynucleotide kinase: gene cloning and enzyme purification. | gene 1 of bacteriophage t4 has been cloned into a lambda pl expression vector, resulting in the overproduction of deoxynucleotide kinase. a procedure that includes affinity chromatography on cibacron blue f3ga-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. the enzyme has been partially characterized in vitro and in vivo, and it appears to be identical to the deoxynucleotide kinase isolated from t4-infected escherichia coli. these results prove the earlier ... | 1990 | 2160930 |
| thyroidal inhibition of growth hormone secretion in fowl: tri-iodothyronine-induced down-regulation of thyrotrophin-releasing hormone-binding sites on pituitary membranes. | the number, but not affinity, of binding sites for [3h]3-methyl-histidine2-trh ([3h]me-trh) on chicken adenohypophysial plasma membranes was increased in chickens made hypothyroid by goitrogen (methimazole) treatment (50 mg/kg per day for 7 days), which also increased circulating gh concentrations. daily i.p. injection of thyroxine (t4; 100 micrograms/kg for 7 days) had no effect on [3h]me-trh binding to pituitary membranes, although it suppressed endogenous gh secretion. binding of [3h]me-trh t ... | 1990 | 2160826 |
| [staging of non-small cell bronchial carcinoma using ct and mrt]. | the tnm classification was rewritten by uicc in 1987. for non-small-cell bronchogenic carcinoma, stages t4 and n3 were redefined. controversy exists in the literature regarding the clinical merits of ct and mri examinations for staging. for the evaluation of very small tumors and lung parenchyma ct is superior to mri. mri, however, permits direct imaging of sagittal and coronal projections. this is favorable in the diagnosis of "superior sulcus tumors" and tumors with chest wall invasion. for th ... | 1990 | 2160670 |
| uv induced (6-4) photoproducts are distributed differently than cyclobutane dimers in nucleosomes. | we have compared the distributions of two stable uv photoproducts in nucleosome core dna at the single-nucleotide level using a t4 polymerase-exonuclease mapping procedure. the distribution of pyrimidine-pyrimidone (6-4) dimers was uncovered by reversing the major uv photo-product, cis-syn cyclobutane pyrimidine dimer, with e. coli dna photolyase and photoreactivating light. whereas the distribution of total uv photoproducts in nucleosome core dna forms a striking 10.3 base periodic pattern, the ... | 1990 | 2160660 |
| effects of oral erythrosine (2',4',5',7'-tetraiodofluorescein) on the pituitary-thyroid axis in rats. | erythrosine (fd&c red dye no.3) is a tetraiodinated derivative of fluorescein. rats fed a 4% erythrosine diet for 30 months beginning in utero have an increased incidence of thyroid adenomas and adenocarcinomas. these tumors may be secondary to increased stimulation of the thyroid gland by tsh. this study was undertaken to determine if dietary erythrosine disrupts the pituitary-thyroid axis thereby altering serum thyroid hormone levels. tsh levels, or the pituitary's response to trh. rats were f ... | 1990 | 2160137 |
| anal intraepithelial neoplasia and anal papillomavirus infection among homosexual males with group iv hiv disease. | ninety-seven male homosexuals with the acquired immunodeficiency syndrome or other group iv human immunodeficiency virus disease were studied for anal human papillomavirus infection and intra-anal cytological abnormalities. human papillomavirus dna was detected in 52 subjects (54%), and 38 subjects (39%) were found to have abnormal anal cytological findings; anal intraepithelial neoplasia was detected in 15 specimens (15%). abnormalities on anal cytological smear were significantly associated wi ... | 1990 | 2160023 |
| cyclic adenosine 3',5'-monophosphate and glucose stimulate thyroxine 5'-deiodinase type ii in cultured mouse neuroblastoma cells. | nutrient modulation increases mouse neuroblastoma (nb) t4-5'-deiodinase ii (t4-5'-d ii) activity. carbohydrates are more potent than either amino acids or glycerol as nutrient sources. glucose rapidly (2 to 4 hours) enhances nb enzyme activity and the response is dependent on new protein synthesis. the present study was performed to further characterize this glucose effect and explore its relationship to the cyclic adenosine monophosphate (camp) system in these cells. nb t4-5'-d ii activity reac ... | 1990 | 2159588 |