Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [a combined treatment with 8 mhz radiofrequency hyperthermia, hpc-adriamycin, immunotherapy, radiation, systemic chemotherapy and irradiation for invasive bladder carcinoma]. | hyperthermia was induced for the treatment of invasive bladder carcinoma in order to study its usefulness. the subjects were 12 cases of invasive bladder cancer; including 5 cases of t2, 3 cases of t3, 2 cases of t4, and 2 cases of recurrence after total cystectomy. as previous treatment, 4 patients received radiotherapy and the other received tur, systemic chemotherapy, and intravesical injection of anticancer drugs. for hyperthermia treatment, a thermotron rf-8 was used for heating a deep seat ... | 1990 | 2232424 |
| seasonal variations in prolactin, growth hormone and thyroid hormones and the prolactin surge at ovulation do not affect litter size of ewes during pregnancy in the oestrous or the anoestrous season. | injection of bromocriptine from 5 days before until 5 days after mating clearly suppressed the periovulatory prolactin surge in ewes in the anoestrous and oestrous season but did not change the litter size significantly. progesterone, gh, tsh or thyroid hormone concentrations were not influenced by the bromocriptine treatment. the progesterone concentrations were lower during the first weeks after mating in the anoestrous season compared to the oestrous season, while there was no difference betw ... | 1990 | 2231554 |
| relationship between train-of-four ratio and first-twitch depression during neuromuscular blockade: a pharmacokinetic/dynamic explanation. | fade, as measured by train-of-four, lags behind twitch depression during the initial phase of nondepolarizing neuromuscular blockade, i.e., the ratio of the fourth to first twitch height in a train (t4/t1) is greater at the onset of the block than during spontaneous recovery for the same level of first twitch depression. we believe that these data can be explained by picturing the muscle as having localized regions that respond much more slowly than the rest, leading to a delay in drug effect in ... | 1990 | 2231323 |
| effect of hapten heterology on thyroid hormone immunoassays. | we examined the effects of hapten heterology on triiodothyronine (t3) and thyroxine (t4) immunoassays configured with protein-conjugated diiodothyronine (t2), t3 or t4, using acridinium ester (ae) as a chemiluminescent label and paramagnetic particles (pmp) as a solid phase. assays constructed with hapten-heterologous combinations, such as immobilized anti-t4 plus labeled t3 or immunobolized anti-t3 plus labeled t2, were superior to the homologous ones in their potential for attaining higher sen ... | 1990 | 2230139 |
| thyroid abnormalities in the mccune-albright syndrome: ultrasonography and hormonal studies. | hyperthyroidism and goiter have been reported frequently in association with the mccune-albright syndrome (mas). to assess the prevalence and extent of thyroid abnormalities in girls with mas, we studied 19 patients [mean age, 6.6 +/- 1 (+/- se) yr; mean bone age, 9.5 +/- 1 yr] and 18 normal control girls (mean age, 10.3 +/- 0.5 yr). all patients appeared euthyroid when examined; 1 was taking antithyroid medication. ultrasonography revealed thyroid abnormalities in 7 patients, including generali ... | 1990 | 2229316 |
| transport of thyroid hormones by human erythrocytes: kinetic characterization in adults and newborns. | the uptake of [125i]t3 and [125i]t4 by human erythrocytes was studied. the erythrocytes were obtained from adult subjects (28-41 yr old) and suspended in a protein-free medium. the half-times of equilibration for both t3 and t4 were 6 min. at equilibrium, t3 was concentrated 55-fold inside the cells, while t4 was concentrated 40 times, but these accumulations were not dependent on either cellular atp or the transmembrane na+ gradient. the amounts of cell-associated thyroid hormones were 20 times ... | 1990 | 2229315 |
| tumor necrosis factor: a putative mediator of the sick euthyroid syndrome in man. | tumor necrosis factor-alpha (tnf) is believed to be an important mediator in many diseases that are associated with the sick euthyroid syndrome. to investigate the effect of tnf on thyroid hormone metabolism, we performed a controlled study in six healthy postabsorptive males, in whom plasma thyroid hormones and tsh were sequentially measured after iv bolus injections of recombinant human tnf (50 micrograms/m2) and isotonic saline. during the 10.5-h study tnf produced the characteristic changes ... | 1990 | 2229314 |
| decreased serum triiodothyronine is associated with increased concentrations of tumor necrosis factor. | previous studies in laboratory animals have shown that tumor necrosis factor-alpha (tnf) may alter thyroid function tests. to determine whether elevated serum tnf levels are associated with altered serum concentrations of t4, t3, free t4, rt3, and tsh, we measured these parameters in 29 nursing home residents with detectable serum tnf levels and compared the levels to those found in 36 patients with undetectable serum tnf levels. the 2 groups were matched for age, sex, clinical problems, use of ... | 1990 | 2229282 |
| thyroid hormones influences sex steroid binding protein levels in infancy: study in congenital hypothyroidism. | in order to test the hypothesis of a role of thyroid hormones on the circulating levels of sex steroid binding protein (shbg) in children, shbg levels were determined in 15 infants with congenital hypothyroidism at diagnosis and during the first 18 months of t4 therapy and in a separate group of 13 children with congenital hypothyroidism (7.1 +/- 0.5 yr, mean +/- sd), treated before 1 month of age, both during adequate l-t4 therapy and 4 weeks after withdrawing therapy. shbg levels were signific ... | 1990 | 2229277 |
| characterization of bacteriophage t4 rega protein-nucleic acid interactions. | the bacteriophage t4 rega protein is a translational repressor of a group of t4 early mrnas. we have characterized the binding of rega protein to polynucleotides and to specific rnas. binding to nucleic acids was monitored by the quenching of the intrinsic tryptophan fluorescence of rega protein. rega protein exhibited differential affinities for the polynucleotides examined, with the order of affinity being poly(ru) greater than poly(dt) greater than poly(du) = poly(rg) greater than poly(rc) = ... | 1990 | 2229058 |
| bacteriophage t4 nrda and nrdb genes, encoding ribonucleotide reductase, are expressed both separately and coordinately: characterization of the nrdb promoter. | we examined the expression of the bacteriophage t4 nrda and nrdb genes, which encode the alpha 2 and beta 2 subunits, respectively, of ribonucleoside diphosphate reductase, the first committed enzyme in the pathway of synthesis of the deoxyribonucleoside triphosphates. t4 nrda, located 700 bp upstream from nrdb, has been shown previously to be transcribed by two major transcripts: a prereplicative, polycistronic message, tu, orginating at an immediate-early promoter, pe, that is 3.5 kb upstream ... | 1990 | 2228963 |
| comparison of two doses of aqueous bovine thyrotropin for thyroid function testing in dogs. | thyroid function was evaluated in 20 healthy dogs by thyrotropin (tsh) response testing. two dose regimens were used: 5 iu of tsh given iv and 1 iu of tsh given iv. blood samples were collected prior to and at 4 and 6 hours after tsh administration. serum was obtained and analyzed for total 3,5,3'-tri-iodothyronine and thyroxine (t4) concentrations by radioimmunoassay. all dogs were classified as euthyroid on the basis of response to 5 iu of tsh at 4 and 6 hours. the 1-iu dose of tsh failed to i ... | 1990 | 2228768 |
| prevalence of thyroid disorders among the elderly in israel. | the prevalence of thyroid disorders was investigated in 698 elderly people (443 women and 255 men), registered in special family health clinics of the israel ministry of health and in two homes for the aged. the first screening was done by serum thyroxine (total t4) examination, and those with values greater than 150 nmol/l or less than 64 nomol/l underwent further laboratory and clinical evaluation in our endocrinological service. hypothyroidism was diagnosed in 12 women and 5 men (2.5% of the ... | 1990 | 2228560 |
| combined cisplatin and radiation therapy in patients with invasive bladder cancer. | thirty-five patients with t2-t4 invasive bladder cancer were treated with combined cisplatin and radiation therapy. in 18 patients radical cystectomy was performed after the combined therapy. in the other 17 patients radical cystectomy could not be performed for various reasons. pathological examination of the cystectomy specimens showed down-staging in 66.7% and no residual tumour in 33.3%. these results suggest a synergistic action of cisplatin and radiation. side effects were not severe and w ... | 1990 | 2228496 |
| effect of triiodothyronine and thyroxine on mitochondrial alpha-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad, bufo melanostictus. | the responsiveness of the adult toad to triiodothyronine (t3) and thyroxine (t4) was studied by measuring the mitochondrial alpha-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad. both t3 and t4 increased the alpha-gpd activity and mitochondrial protein content of liver and muscle of toad. the extent of increase in the alpha-gpd activity and mitochondrial protein content were more pronounced with t3 than with t4. further that the muscle ... | 1990 | 2227800 |
| human immunodeficiency virus infection and cervical neoplasia. | to determine the relationship between human immunodeficiency virus (hiv) infection and cervical neoplasia, the characteristics of invasive and preinvasive cervical disease in 114 patients of known hiv status were assessed. seven of thirty-seven patients (19%) under age 50 with invasive cervical carcinoma were hiv-positive, including a 16-year-old with stage iiib disease. hiv-positive patients had more advanced invasive cancer than hiv-negative patients. disease persisted or recurred in all hiv-p ... | 1990 | 2227552 |
| studies on the thyroid in transgenic mice expressing the genes for human and bovine growth hormone. | the thyroid glands of transgenic mice (tm) expressing the genes for human (h) and bovine (b) growth hormone (gh) were studied. the percentages of larger follicles in hgh tm of either sex were significantly greater than in the corresponding normal littermates, and follicles ranging up to 350 microns in diameter were present in male hgh tm. in contrast, thyroid follicles were only slightly enlarged in male bgh tm, and were unchanged in female bgh tm. the serum concentrations of t4 were significant ... | 1990 | 2226720 |
| mesial temporal sclerosis: pathogenesis, diagnosis, and management. | mesial temporal sclerosis (mts) is probably the most common symptomatic pathologic entity--alone or mixed with other pathologic features--for seizures of temporal lobe origin. the pathophysiology of mts, including any genetic influence, needs clarification. a characteristic ictal expression for seizures of mts origin appears not to exist. the majority of patients (78%) with postresection mts who are seizure-free have tightly localized interictal abnormalities restricted to f7/f8, sp1/sp2, t3/t4, ... | 1990 | 2226372 |
| prolonged effects of recombinant human interleukin-1 alpha on mouse thyroid function. | rapid and prolonged effects of recombinant human interleukin-1 alpha (il-1 alpha) on mouse thyroid were studied. after daily administration of 15 micrograms or 1 microgram il-1 for 7 consecutive days, serum t4 concentrations rapidly fell to undetectable levels but returned to near control level after the cessation of il-1. on the 31st day, 3 weeks after the drug cessation, a significant depression of serum t4 was observed again. in addition, the il-1-treated mouse thyroid showed an in vitro unre ... | 1990 | 2226318 |
| effect of thyroxine administration on the expression of epidermal growth factor in the kidney and submandibular gland of neonatal mice. an immunocytochemical and in situ hybridization study. | the effect of t4 administration on epidermal growth factor (egf) expression in kidney and submandibular glands (smg) was studied in newborn mice. egf messenger rna (mrna) abundance and egf immunoreactivity were assessed by in situ hybridization and immunohistochemical techniques, respectively. t4 treatment on days 0-6 augmented both the in situ hybridization and immunocytochemical signals in kidney but not in smg on day 7. by contrast, t4 injections on days 7-14 did not alter the in situ hybridi ... | 1990 | 2226315 |
| localization of the thyroxine binding sites in apolipoprotein b-100 of human low density lipoproteins. | by photoaffinity labeling human low density lipoproteins (ldl) with [125i]t4 we confirmed our previous observation that of the three t4 binding sites of apolipoprotein b-100 (apob-100) one is in its 26% nh2-terminal portion [apob-26, the 140 kda fragment, residues 1-1297] and two in the remaining 74% cooh portion (apob-74, 410 kda, residues 1298-4536). we now show that of these two sites one is in the nh2 portion of apob-74 (apob-44, 240 kda, residues 1298-3249) and the other in the nonoverlappi ... | 1990 | 2226312 |
| thyrotropin receptors, thyroid hormones and thyroglobulin in patients with hashimoto's thyroiditis. | tsh receptors in plasma membranes of thyroid nodular and perinodular tissue and the concentration of thyroid hormones and thyroglobulin in serum were estimated in patients with hashimoto's thyroiditis (ht) and in six persons with scintigraphically cold thyroid nodules (afn). in perinodular tissue of ht patients specific binding of 125i-btsh was found to be significantly decreased (1.50 +/- 0.13%; mean +/- s.e.) as compared with afn patients (2.79 +/- 0.53%), whereas any significant difference in ... | 1990 | 2226281 |
| influence of melatonin on thyroid secretion in pinealectomized rats. | the influence of a single dose of melatonin administered to pinealectomized and sham operated rats at different times of day on serum t3, t4 concentrations was investigated. it was found that such melatonin influence depends upon a time of day. both pinealectomy and exogenous melatonin did not influence the thyroid activity in the morning. the most remarkable pineal effect on the thyroid appeared during the night. | 1990 | 2226279 |
| the effect of amiodarone on the control of hyperthyroidism by propylthiouracil. | we studied the efficacy of amiodarone (800, 600, 400 and 200 mg orally daily during weeks 1, 2, 3 and 4, respectively) plus propylthiouracil (ptu, 100 mg orally every 8 h) in comparison to ptu alone in the early treatment (28 days) of graves' disease patients. circulating t3 and t4 decreased earlier and more markedly in the amiodarone plus ptu-treated group. an initial rise of circulating rt3 above the base-line value followed by a gradual decline was observed in the former group while only a de ... | 1990 | 2225478 |
| increased survival of patients with massive lymphadenopathy and prostate cancer: evidence of heterogeneous tumour behaviour. | the survival of patients with prostate cancer and radiologically detectable lymph node enlargement has been studied prospectively over an 8-year period. computed tomography in 108 patients presenting with symptoms, signs or biochemical results suggesting lymphatic spread revealed pelvic or abdominal node masses in 60 patients; in 29 (48%), the masses measured more than 4 cm and the maximum node diameter was 15 cm. two-thirds of patients had advanced (t3/t4) tumour stage. following treatment, act ... | 1990 | 2224436 |
| unique hairpin structures occurring at the replication origin of phage g4 dna. | recently we reported that a dna fragment, gccaaagc, forms an extraordinarily stable hairpin structure with two g-c pairs at the terminus and a-a-a stacked structure. the sequence is present at the replication origin of bacteriophage g4 ssdna, and so on. several kinds of possible hairpin structures, corresponding to the replication origin of phage g4, were synthesized and their secondary structures were examined. it was found that the fragments are able to form interconvertible hairpin structures ... | 1990 | 2223881 |
| mechanistic studies of ionizing radiation and oxidative mutagenesis: genetic effects of a single 8-hydroxyguanine (7-hydro-8-oxoguanine) residue inserted at a unique site in a viral genome. | t4 rna ligase was used to construct a deoxypentanucleotide containing a single 8-hydroxyguanine (7-hydro-8-oxoguanine; g8-oh) residue, which is one of the putatively mutagenic dna adducts produced by oxidants and ionizing radiation. the pentamer d(gctag8-oh)p was prepared by the ligation of a chemically synthesized acceptor molecule, d(gcta), to an adducted donor, 8-hydroxy-2'-deoxyguanosine 5',3'-bisphosphate. the acceptor was efficiently converted to the reaction product (greater than 95%), an ... | 1990 | 2223758 |
| prolonged paralysis following suxamethonium and the use of neostigmine. | a case of prolonged neuromuscular block following the administration of suxamethonium is reported. three hours after administration of suxamethonium, a well defined, recovering phase ii block was demonstrated with a t4:t1 ratio of 0.25, and neostigmine was administered. although the t4:t1 ratio was improved to 0.9, t1 remained at 25% of control, and significant paralysis persisted which responded to administration of cholinesterase. it is concluded that neuromuscular monitoring cannot reliably p ... | 1990 | 2223373 |
| use of atp, datp and their alpha-thio derivatives to study dna ligase adenylation. | bacteriophage-t4 and human type i dna ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35s]thio-atp. however, the joining reaction does not take place in the presence of the deoxyribotriphosphates. enzyme adenylation is reversed in all cases by an excess of ppi, but the rate of reversion is lower with thio derivatives. therefore thio derivatives can be used to study the adenylation of dna ligases and to search for specific inhibitors of the first st ... | 1990 | 2222417 |
| [accuracy in thyroid hormonology: evaluation of free t4 by five immunometric methods using cold markers]. | during a comparative multicentric evaluation, five commercially available non-isotopic methods for ft4 measurement were tested. as with the radioactive methods the problem with accuracy is again evident. this is true both for setting reference values and standards as well as for the specificity of the methods. the authors demonstrated highly significant correlations as described in recent papers: many of the values may, however, be up to double value in one center compared to another despite the ... | 1990 | 2221497 |
| treatment selection for carcinoma of the base of the tongue. | between 1974 and 1984, 173 patients were treated for squamous cell carcinoma of the tongue base. fifty-four patients had t1 or t2 primaries, while 115 patients had t3 or t4 tumors (4 were not staged). lymph node metastasis was present in 120 patients. early primary tumors treated with surgery or radiotherapy had a control rate of 83% (5 of 6 tumors) and 89% (40 of 45 tumors), respectively. for advanced primary tumors, definitive radiotherapy produced a local control rate of 55% (42 of 76 tumors) ... | 1990 | 2221246 |
| significance of positive margins in oral cavity squamous carcinoma. | three hundred ninety-eight consecutive, previously untreated patients undergoing surgery for epidermoid carcinoma of the oral cavity from 1979 to 1983 were reviewed. one hundred twenty-nine patients were classified as having positive surgical margins. of these, 83 patients had tumor within 0.5 mm of the surgical margin, 9 had premalignant changes at the margin, 9 had in situ carcinoma at the margin, and 28 had invasive cancer at the margin. the remaining 269 patients had uninvolved margins. the ... | 1990 | 2221245 |
| circadian rhythmicity of rate-normalized qt interval in hypothyroidism and its significance for development of class iii antiarrhythmic agents. | lengthening of repolarization and refractoriness occurs in hypothyroidism; it is associated with a reduced probability of arrhythmias, with the converse occurring in hyperthyroidism. because the qt interval and its circadian rhythmicity relative to heart rate is poorly defined in man, we used our new computer-assisted technique to measure qt interval in our analysis of 24-hour holter tapes before and after (8 to 12 weeks) thyroxine (t4) replacement in 10 patients with hypothyroidism; the finding ... | 1990 | 2220619 |
| enhanced chemiluminescence in the measurement of proteins and haptens: evaluation of choriogonadotropin (hcg) and free thyroxin. | we evaluated the amerlite system (amersham, bucks, uk) for hcg and ft4. the within-run imprecision (cv%) for hcg was 4.05 at 19.6 u/l (n = 10), 6.28 at 43.45 u/l (n = 10) and 4.62 at 298.57 u/l (n = 10). the between-run imprecision (five replicates for ten days) was 4.8%, 15% and 11%, respectively. the system was linear up to 200 u/l. a good correlation between amerlite hcg and an irma assay (becton dickinson, r = 0.91), delfia (pharmacia, r = 0.91) and an automated elisa assay on es 600 (boehri ... | 1990 | 2220417 |
| [plasma membrane separation. a therapeutic possibility in thyrotoxic crisis]. | a case of thyroid storm is presented, successfully treated with a combination of antithyroidal drugs and plasma exchange. a 280% separation removed 447 micrograms t4 and 12 micrograms t3. during separation, the elevated t4 and t3 values returned to normal. basic concepts of plasma membrane separation investigated by plasma and whole body counting after administration of 131i-t4, as well as radioimmunoassay measurement of thyroid hormones in plasma and separate are discussed. | 1990 | 2220268 |
| [regulation of triiodothyronine levels in fatty tissue in obese patients]. | specific activity of t4-5'-deiodinase, the enzyme which catalyze peripheral production of triiodothyronine (t3) from thyroxine (t4) has been evaluated in the subcutaneous adipose tissue of obese patients. in lean healthy women (bmi less than 21) mean activity of t4-5'-d was found to be 122 +/- 29.4 fmol t3/mg of protein/min and was higher then respective activity of the enzyme in adipocytes of patients with mild obesity (22 less than bmi less than 30) where the mean was 92.14 +/- 18.05 fmol t3/m ... | 1990 | 2219931 |
| [subclinical hypothyroidism]. | subclinical hypothyroidism was detected in 5.7% of 853 patients referred for ambulatory examination to a department in prague. in patients referred with a mild degree of subclinical hypothyroidism with an evaluated level of thyrotropic hormone in serum (tsh) no significant deviations of serum thyroxine and triiodothyronine (t4 and t3) were found nor different values of the achilles tendon reflex time, as compared with patients with normal thyroid function. in case of a more marked grade of subcl ... | 1990 | 2219763 |
| immune status and immune therapy of renal cell carcinoma. | at present, no sufficient therapy for metastatic renal cell carcinoma is available. several immunotherapeutical protocols have been studied, success rates, however, were inconsistent. the purpose of this study was to assess the pretherapeutic immunological status of 13 patients with metastatic and 16 patients with nonmetastatic renal cell carcinoma and of 15 healthy volunteers. determined were differential blood counts, lymphocyte subpopulations, beta 2-microglobulin, tumor necrosis factor (tnf) ... | 1990 | 2219564 |
| evidence for susceptibility of intrathymic t-cell precursors and their progeny carrying t-cell antigen receptor phenotypes tcr alpha beta + and tcr gamma delta + to human immunodeficiency virus infection: a mechanism for cd4+ (t4) lymphocyte depletion. | individuals infected by the human immunodeficiency virus type 1 (hiv-1) demonstrate progressive depletion and qualitative dysfunction of the helper t4 (cd4+) cell population. mechanisms proposed for attrition of cd4+ t cells include direct cytopathicity of these mature cells following infection as well as infection of early t-lymphocyte progenitors. the latter mechanism could lead to failure to regenerate mature functioning cd4+ t cells. the present study determines the susceptibility of thymocy ... | 1990 | 2217206 |
| dna polymerase ii is encoded by the dna damage-inducible dina gene of escherichia coli. | the structural gene for dna polymerase ii was cloned by using a synthetic inosine-containing oligonucleotide probe corresponding to 11 amino acids, which were determined by sequencing the amino terminus of the purified protein. the labeled oligonucleotide hybridized specifically to the lambda clone 7h9 from the kohara collection as well as to plasmid pgw511 containing the sos-regulated dina gene. approximately 1400 base pairs of dina sequence were determined. the predicted amino-terminal sequenc ... | 1990 | 2217198 |
| klenow polymerase is preferable to t4 polymerase in end-labeling reactions. | 1990 | 2216799 | |
| [changes in the thyroid status of school children in relation to improved dietary iodine intake]. | five years after the introduction of the iodine prophylaxis examinations on the reaction of the thyroidal state were carried out in 150 children, 9 to 15 years of age in a district of the erzgebirge in the south of the gdr. the results of the t3-, t4- and tsh-concentrations within the serum demonstrated in all the children an euthyroid metabolic condition. the medium renal iodine elimination of 37.3 +/- 4.7 micrograms iodine/g creatinine still resulted in an ionic deficiency of 2nd degree. the t ... | 1990 | 2216529 |
| [changes in lymphocyte subpopulations induced by the administration of thymopentin]. | modifications of the levels of t-helper (t4) and t-suppressor (t8) lymphocytes were assessed, together with their ratio, in 17 subjects who had received thymopentine as an adjuvant to hevac-b vaccination. two months after treatment with the immunomodulating drug, t4 lymphocytes were considerably increased whereas absolute levels of t8 lymphocytes were decreased in the majority of patients: this led to a net increase in the t4/t8 ratio. this finding leads to the conclusion that all subjects produ ... | 1990 | 2216015 |
| intron-associated splicing reactions in bacteriophage t4. | group i introns are present in at least three bacteriophage t4 genes: td, nrdb and suny. the transcription products of these three genes have similar intron consensus regions and secondary structures, which render them capable of guanosine-mediated in vitro autocatalytic splicing reactions. moreover, it has been shown that the 245-amino-acid protein encoded in the td intron expresses an endonuclease that cleaves near the joining site for the two exons in the intron-deleted thymidylate synthase g ... | 1990 | 2215214 |
| clinical classification and staging for primary malignancies of the maxillary antrum. | a study of 51 patients with primary malignant maxillary sinus neoplasms was conducted. none of the patients had neck nodes and/or metastases, and each had 5-year follow-up. the tumors were staged according to the 1983 and 1988 american joint committee on cancer staging systems for maxillary sinus cancers. there were 13 early stage (t1, t2) and 38 advanced (t3, t4) tumors in both systems. cox regression analyses of survival curves showed increasingly worse prognoses for advanced tumors in both t- ... | 1990 | 2215044 |
| [functional activity of the pituitary-thyroid gland system during 370-day anti-orthostatic hypokinesia]. | the concentrations of the thyrotrophic hormone (tth). bound thyroxine (t4), free thyroxine (ft4), and triiodothyronine (t3) were measured by ria in the blood of 10 test subjects during a 370-day head-down tilt study and a 250-day recovery period after it. the concentrations of tth, ft4, and t3 decreased during bed rest and did not return to norm during recovery. it can be assumed that during bed rest tth stimulation of the thyroid gland declines, which can act as an adaptive response to lower ph ... | 1990 | 2214662 |
| [studies of the morphology of the thyroid gland and thyroid hormone levels in the blood of rats in experiments on "kosmos-1667" and "kosmos-1887"]. | using histological, electron microscopic, and biochemical (measurement of total thyroxine, free thyroxine and triiodothyronine in plasma) method, thyroid glands of 17 male rats of the wistar spf strain flown for 7 days on cosmos-1667 and for 13 days on cosmos-1887 were investigated. it was found that a longer exposure to space flight effects (for 13 days) led to a thyroid activity decline (significant reduction of thyrocyte size and nuclear area, accumulation of colloid drops in the cytoplasm, d ... | 1990 | 2214661 |
| taiwan's first case of perinatal transmission of hiv confirmed by a modified western blot test. | a 25-year-old chinese woman, was found hiv antibody positive on december 14, 1988. during our follow-up, we tested her american husband and found him to be seronegative. unfortunately, her six-month-old infant was seropositive. the standard western blot test was used in the first stage of analysis. the bands which appeared on the infant's strip were p15, p24, p31, p55 and gp120/gp160, but using the modified western blot test the bands which appeared were p15, p24, p31, gp41, p53, p55, p64 and gp ... | 1990 | 2213974 |
| effects of chlorine dioxide on the developing rat brain. | male and female long-evans rat pups, exposed to an oral dose of 14 mg chlorine dioxide (cio2)/kg/d (postnatal d 1-20), were examined for effects on brain development and for changes in thyroid activity. body weight reductions were observed on postnatal (pn) d 11, 21, and 35. forebrain weight and protein content were decreased on pnd 21 and 35, as were the dna content on d 35 and the number of dendritic spines on cerebral cortical pyramidal cells, a marker for synapse formation. otherwise, cell p ... | 1990 | 2213920 |
| [investigation of the immune response in tonsillar lymphocytes against streptococci in patients with pustulosis palmaris et plantaris]. | the purpose of this study was to clarify the etiological role of immune responses in tonsillar lymphocytes of the patients with pustulosis palmaris et plantaris (ppp). in order to determine their immune response to s. pyogenes t4, s. pyogenes t12, s mitis, s. salivarius, s. sanguis i, s. sanguis ii and s. pneumoniae, tonsillar lymphocytes from patients with ppp, ct, and sleep apnea syndrome (control) were cultured with formalinized streptococcal cellular antigens. blastoid transformation was inv ... | 1990 | 2213357 |
| evaluation of a new enzyme immunoassay system for free thyroxine (enzymun-test ft4). | free thyroxine (ft4) represents the metabolically active fraction of the circulating thyroid hormone thyroxine (t4). in this paper the results of the evaluation of a newly developed ft4 assay are reported. this assay system is based on an enzyme-labelled one-step immunoassay. the within-run imprecision, checked using serum pools and several commercial reference materials, showed a coefficient of variation (cv) of between 0.8 and 9.8%, depending on the reference material used. the between-run imp ... | 1990 | 2212960 |
| [clinical study of preoperative radiotherapy of bladder cancer]. | from may 1982 to nov. 1987, 33 patients with bladder carcinoma were treated with preoperative radiotherapy (20 gy/5fr) and total cystectomy. the over all 3-year survival rate was 70%. for t1 and t2, 3-year survival rate was 100%, but only 55% and 0% for t3 and t4 respectively. in 23 out of 33 patients, preoperative t-stage was confirmed by tur-bt. down-staging was recognized in 7 out of 23 patients (30%). they were 0 out of 1 patients for tcis (0%), 2 of 3 for t1 (67%), 3 of 6 for t2 (50%), 2 of ... | 1990 | 2212829 |
| [surgical treatment of ischemic heart disease combined with hypothyroidism]. | treatment for patients with ischemic heart disease and hypothyroidism contains many difficulties, such as a dilemma that thyroid hormone to hypothyroid patients may worsen angina. the purpose of this study is to propose an appropriate control of thyroid function in these patients before coronary artery bypass grafting (cabg), and to clarify the change of thyroid function during postoperative period. because of progressive angina pectoris, five hypothyroidism patients underwent cabg. preoperative ... | 1990 | 2212771 |
| thyroid function in leprosy. | thyroid function tests were carried out in 43 cases of leprosy. the study subjects included cases of tuberculoid, borderline and lepromatous leprosy and those with lepra reaction. the parameters studied included serum cholesterol, protein bound iodine, serum t3 level and serum t4 levels. the levels of serum cholesterol and protein bound iodine were normal in all the four groups of leprosy patients. however, the mean serum t3 and t4 were low in all the four groups. the difference in the levels of ... | 1990 | 2212737 |
| [the value of cytological grading of prostatic adenocarcinoma. comparison of the clinical state, the gleason score and grade in 69 cases]. | during a two-year period, 69 positive prostatic fine-needle cyto-aspirates were selected for a comparison between the cytological grade and the clinical data, and also with the gleason score and grade obtained with needle-biopsy. the cytological grade is independent of stage, except for grade iii that indicates an advanced tumor (t3-t4). on the other hand, the cytological grade provides valuable indications on the histopathological differenciation according to gleason, with no grade ii inferior ... | 1990 | 2212719 |
| [nodular thyreopathies. results of 1300 operations]. | to evaluate the results of surgery for nodular thyreopathies, 1300 cases operated on from january 1974 to december 1987 were reviewed. 842 patients (64.7%) were female; the average age, 41 +/- 2.4 year. thyroid pathology was represented by solitary nodule in 643 cases (49.4%); multinodular goitre in 559 cases (43.0%) (377 euthyroid and 182 hyperthyroid patients); retrosternal in 78 cases (5.9%); recurrent goitre in 20 cases (1.5%). the patients underwent total thyroidectomy (525 cases); sub-tota ... | 1990 | 2211891 |
| serum thyroid hormone concentrations in clinically normal dogs after administration of freshly reconstituted vs previously frozen and stored thyrotropin. | concentrations of serum thyroxine (t4) and 3,3',5-triiodothyronine (t3) were determined in 7 clinically healthy adult dogs before and after administration of freshly reconstituted thyrotropin (tsh) and tsh that had been previously reconstituted and frozen for 1, 2, and 3 months. the 4 tsh response tests were performed at 30-day intervals by collecting blood samples for serum t4 and t3 determinations before and 4 and 6 hours after iv administration of tsh (0.1 u/kg of body weight). baseline serum ... | 1990 | 2211306 |
| combined 5-fluorouracil and irradiation for transitional cell carcinoma of the urinary bladder. | thirty-four patients have completed treatment on a bladder-preservation protocol using primary irradiation combined with infusion 5-fluorouracil (5-fu). 4,000 cgy pelvic irradiation was delivered in 5 weeks, with 1,000 mg/m2/day of 5-fu administered as a 96 hr infusion on days 1-4 of week 1 and 4. after a 3-week rest period, patients eligible for cystectomy underwent cystoscopy and biopsy. those with residual tumor underwent cystectomy, and those without tumor received an additional cycle of che ... | 1990 | 2211217 |
| radiotherapy with and without cisplatin in bladder cancer. | from october 1985 to december 1988, 67 patients with invasive bladder carcinoma t1-4 n0-2 m0 were treated with irradiation (50.4 gy in 28 fractions in 6 weeks) and simultaneous cisplatin (25 mg/m2 per day on 5 consecutive days in the first and fifth irradiation week). after transurethral resection and irradiation plus cisplatin, complete remissions were achieved in 8/11 t1-, 14/16 t2-, 27/36 t3- and 1/4 t4-tumors. the complete remission rate 6 weeks after treatment according to the extent of pre ... | 1990 | 2211216 |
| basement membrane type iv collagen in oral squamous cell carcinoma. | biopsied specimens of 82 oral squamous cell carcinomas were immunohistochemically studied for detection of type iv collagen existence in the basement membrane (bm). immunoreactivity on bm was semiquantitatively evaluated as limited (+/-), moderate (+), or extensive (+ +), and matched against clinical and histopathologic features. no clear difference in bm deposition could be found in the tumor sites. the amount of type iv collagen decreased in advanced t stage (t3 and t4) tumors. bm deposition w ... | 1990 | 2211100 |
| thyrotropin (tsh) action on thyroid hormone deiodination and secretion: one aspect of thyrotropin regulation of thyroid cell biology. | tsh, the major signalling factor for the thyroid follicles, controls thyrocyte function in concert with other modulators of cell growth, differentiation and structural organization of the follicular-endothelial network. most of the tsh effects are mediated by tsh binding to the tsh receptor which stimulates adenylate cyclase catalyzed camp production. tsh is involved in the regulation of thyroidal uptake of small molecules and nutrients, intracellular transport of thyrocyte specific proteins, an ... | 1990 | 2210628 |
| preoperative staging and risk analysis in esophageal carcinoma. | in esophageal cancer surgery a preoperative risk analysis of the patient and staging of the tumor are necessary to reduce postoperative mortality and to identify those patients who will benefit from primary surgery. a risk analysis includes the recording of cardiac, pulmonary, renal, hepatic and cerebral functions, and defines the functional limits of the various systems. preoperatively it is most important to assess whether the primary tumor is completely resectable or not, because only patient ... | 1990 | 2210604 |
| [changes in tsh-receptor antibody (tr-ab) and thyroid stimulating antibody (ts-ab) after thyroidectomy in thyrotoxic patients]. | changes in tsh-receptor antibody (tr-ab) and thyroid stimulating antibody (ts-ab) after thyroidectomy were examined in seventeen thyrotoxic patients (3 males and 14 females, 40.0 +/- 3.4 yr) with positive tr-ab and ts-ab. they were subjected to thyroid surgery because of suspected malignancy, methymazol induced agranulocytosis, cardiac failure, recurrent gastric ulcer or emotional instability. of these patients, 3 were totally thyroidectomized, 11 were subtotally thyroidectomized and 3 were unil ... | 1990 | 2209922 |
| t lymphocytes in non-malignant, pre-malignant and malignant changes of the cervix. | t lymphocytes play an important role in the immune response to both neoplasia and viral infection. normally about 60% of t lymphocytes in the peripheral blood are t4 lymphocytes, while 20-30% are t8 ones. the t4/t8 ratio is usually 2:1. when this ratio is inverted the most significant changes occur. the aim of our study was to examine the t4/t8 lymphocyte ratio in the peripheral blood of patients with histologically confirmed non-malignant and premalignant changes of the cervix and to compare it ... | 1990 | 2209637 |
| normal thyroid function values in ethiopians. | thyroid function values were determined in 56 healthy nongoitrous adult ethiopians. the mean triiodothyronine (t3) values for 20 males and 36 females were 1.42 +/- 0.32 nmol/l and 1.51 +/- 0.25 nmol/l, and thyroxine (t4) values were 119 22 nmol/l and 116 +/- 21 nmol/l respectively. the mean thyrotropin (tsh) values for males and females were identical at 1.85 +/- 0.94 mu/l. radioactive iodine uptake (raiu) at 2 hours was 8.6 +/- 4.4% in males and 11.3 +/- 4.3% in females, and at 24 hours 31.7 +/ ... | 1990 | 2209581 |
| management of primary hypothyroidism. | primary hypothyroidism is a common condition requiring lifelong treatment and monitoring. the type and amount of thyroid hormone replacement, selection of laboratory tests, and timing of office visits are all important for optimizing patient well-being and reducing the costs of medical care. the aim of treatment is to bring the patient to the euthyroid state. currently this is defined as a normal serum concentration of tsh by recently developed sensitive and specific immunometric assays, and is ... | 1990 | 2208954 |
| bone scintigraphy in nasopharyngeal carcinoma. | one hundred and forty-three patients (group 1) with histologically proven nasopharyngeal-carcinoma (npc) had bone scintigraphy with 99tcm methylenediphosphonate (mdp) or dihydroxypropanediphosphonate (dpd) within 2 months of the initial diagnosis. a further 162 patients (group 2) had bone scans during the course of follow-up if there were symptoms of bone pain or evidence of metastases at other sites. twenty-three per cent (33/143) of the newly diagnosed npc patients (group 1) had evidence of bo ... | 1990 | 2208925 |
| a clinical and flow cytometric analysis of patients with nasopharyngeal cancer. | abnormal cellular dna content, a hallmark of malignancy, is known to be an important prognostic factor in many human solid tumors; however, no data have been published on whether cellular dna content carries prognostic significance for patients with nasopharyngeal cancer (npc). archival, formalin-fixed, paraffin-embedded pathology specimens representing pretreatment tissue biopsies from 55 patients (41 men and 14 women) with npc were analyzed for cellular dna content in a retrospective fashion f ... | 1990 | 2208035 |
| mapping of serotonin-like immunoreactivity in the ventral nerve cord of crayfish. | whole-mount immunohistochemical technique using antibody to serotonin (5-ht) had been used to map presumptive serotoninergic structures in the ventral abdominal and thoracic nerve cord of crayfish procambarus clarkii. 5-ht immunoreactivity was detected in more than 30 cell bodies, numerous fibers and peripheral nerve endings of root plexus and neuropilar regions. immunoreactive fibers are arranged in 3 pairs of rostrocaudal bundles. the median (mfb) and the lateral fiber bundles run longitudinal ... | 1990 | 2207659 |
| two-site immunoassays for the measurement of serum laminin: correlation with breast cancer staging and presence of auto-antibodies. | 1. binding to and destruction of basement membrane (bm) are necessary steps for cancer cells to extravasate and metastasize. serum levels of released bm components may correlate with the staging of human cancers or with inflammatory disorders. furthermore, released material may also induce autoantibodies. since laminin, an 800-kda glycoprotein, is present in the extracellular matrix, serum laminin levels may be markers of bm injury. 2. a two-site enzyme immunoassay and a radioimmunoassay were de ... | 1990 | 2207442 |
| lack of association between thyroid function and mitral valve prolapse in graves' disease. | 1. to determine whether the association between mitral valve prolapse and graves' disease is related to thyroid function, three groups of individuals were studied: 16 patients with graves' disease and hyperthyroidism (hyperthyroid: t4 greater than 11.5 micrograms/100 ml), 16 patients with graves' disease without hyperthyroidism (euthyroid: t4 less than 11.5 micrograms/100 ml), and 40 healthy individuals. the three groups were similar in age, sex distribution, and anthropometrical characteristics ... | 1990 | 2207441 |
| human immunodeficiency virus and the gastrointestinal tract. | human immunodeficiency virus (hiv) is a retrovirus infecting cd4 positive cells, causing profound immunosuppression and eventually manifesting clinically as the acquired immunodeficiency syndrome (aids). the cells principally infected by hiv are t4 (helper) lymphocytes and macrophages. the eventual loss of helper cell function is the prime reason for immunodeficiency, which renders the individual susceptible to opportunistic infections. virtually every organ system in the body can be affected cl ... | 1990 | 2207349 |
| analysis of melting transitions of the dna hairpins formed from the oligomer sequences d[ggatac(x)4gtatcc] (x = a, t, g, c). | optical melting transitions of the short dna hairpins formed from the self-complementary dna oligomers d[ggatacx4gtatcc] where x = a, t, g, or c measured in 100 mm nacl are presented. a significant dependence of the melting transitions on loop sequence is observed and transition temperatures, tm, of the hairpins vary from 58.3 degrees c for the t4 loop hairpin to 55.3 degrees c for the a4 loop. a nearest-neighbor sequence-dependent theoretical algorithm for calculating melting curves of dna hair ... | 1990 | 2207283 |
| assignment of the backbone 1h and 15n nmr resonances of bacteriophage t4 lysozyme. | the proton and nitrogen (15nh-h alpha-h beta) resonances of bacteriophage t4 lysozyme were assigned by 15n-aided 1h nmr. the assignments were directed from the backbone amide 1h-15n nuclei, with the heteronuclear single-multiple-quantum coherence (hsmqc) spectrum of uniformly 15n enriched protein serving as the master template for this work. the main-chain amide 1h-15n resonances and h alpha resonances were resolved and classified into 18 amino acid types by using hmqc and 15n-edited cosy measur ... | 1990 | 2207079 |
| domain structure in yeast trna ligase. | yeast trna ligase is one of two proteins required for the splicing of precursor trna molecules containing introns. the 95-kda trna ligase has been purified to homogeneity from a strain of escherichia coli which overexpresses the protein. the ligation reaction requires three enzymatic activities: phosphodiesterase, polynucleotide kinase, and ligase. by partial proteolytic digestion, we have produced fragments of trna ligase which contain the constituent activities. these results provide evidence ... | 1990 | 2207062 |
| carcinoma of the nasopharynx. a retrospective analysis of treatment results in 125 patients. | from 1958 to 1982, 125 patients with histologically verified carcinoma of the nasopharynx were treated with external radiotherapy as the sole curative intent. the 5-year actuarial survival rates by stage were as follows: stage i 68%, stage ii 64%, stage iii 44%, and stage iv 20%. primary control rate by t-category was 71% in t1, 67% in t2, 52% in t3, and 64% in t4. regional control rate was 96% in n0, 76% in n1, 61% in n2, and 63% in n3. the t-category was found to be an important prognostic par ... | 1990 | 2206575 |
| structure and fluctuations of bacteriophage t4 glutaredoxin modelled by molecular dynamics. | a 120 ps molecular dynamics (md) trajectory for bacteriophage t4 glutaredoxin was calculated including non-inertial solvent effects. the potential energy attains an equilibrated regime after the first 20 ps. the r.m.s. difference of all non-hydrogen atoms between x-ray and average md structures for the regular secondary structure is 0.99a which shows that the md simulation reproduces the essentials of the structure with high accuracy. loop displacements are detected, shown by the larger full str ... | 1990 | 2206129 |
| frameshift suppression at tandem aga and agg codons by cloned trna genes: assigning a codon to argu trna and t4 trna(arg). | arginine is coded for by cgn (n = g, a, u, c), aga and agg. in escherichia coli there is little trna for aga and agg and the use of these codons is strongly avoided in virtually all genes. recently, we demonstrated that the presence of tandem aga or agg codons in mrna causes frameshifts with high frequency. here, we show that phaseshifts can be suppressed when cells are transformed with the gene for trna(t4arg) or e. coli trna(argu,arg) demonstrating that such errors are the result of trna deple ... | 1990 | 2205835 |
| local infiltration of t-lymphocyte subsets as a prognostic indicator in patients with nasopharyngeal carcinoma. | we studied tumor-host interactions in 47 patients with npc. the local infiltration of t-lymphocyte subsets was investigated by an immunoperoxidase technique using monoclonal antibodies. biopsy specimens of patients without cervical metastasis showed more t-lymphocyte (t11) infiltration. the amount of leu-3a (helper/inducer) and t8 (cytotoxic/suppressor) cell infiltration did not correlate with the age, sex, clinical stage, and peripheral blood t4 and t8 cells of the patients. a higher incidence ... | 1990 | 2204526 |
| electroinsertion of full length recombinant cd4 into red blood cell membrane. | electroinsertion is a novel technique of protein implantation in cell membranes using electrical pulses, of field strength between 1.3 kv/cm and 2.1 kv/cm and up to 1 ms duration. the full length recombinant cd4 receptor could thus be inserted in human and murine red blood cell (rbc) membranes. 100% of the rbc subjected to this procedure were shown to expose different cd4 epitopes after electroinsertion. an average of 5000 epitopes per cell has been detected by immunofluorescence assay using flo ... | 1990 | 2204427 |
| intrauterine diagnosis and treatment of fetal goitrous hypothyroidism. | newborn screening programs for the detection of congenital hypothyroidism have dramatically shortened the time before treatment is begun. however, concern still exists about central nervous system sequelae which may persist due to a period of untreated intrauterine hypothyroidism. presence of polyhydramnios led to the ultrasound diagnosis of a fetal goiter. hypothyroidism was confirmed at 34 weeks gestation by percutaneous fetal blood sampling, which revealed an elevated tsh (186 mu/l) and a low ... | 1990 | 2203798 |
| analysis of leukocyte surface antigens on ethanol-fixed paraffin-embedded tissue material. | ethanol-fixed paraffin-embedded specimens of human tissues were studied whether the surface antigens of leukocytes in these tissues can be stained and analyzed. three-layer indirect immunoperoxidase staining was performed on the ethanol-fixed paraffin-embedded sections by the use of several monoclonal antibodies for whole human leukocytes (dako lc), b cells (dako cd-22, 4kb5, and l26; leu 14), t cells and their subsets (dako uchl-1, t1, t3, t4 and t8; leu 4, 3a and 2a) and monocyte/macrophage li ... | 1990 | 2202760 |
| the mechanism for the discrepancy between serum total and free thyroxine values induced by autoantibodies: report on two patients with graves' disease. | the sera from two patients with graves' disease gave abnormally high serum free t4 values as compared with the total t4 and other hormone values, suggesting the presence of autoantibodies to labelled t4 analogue used in the amersham free t4 assay kit. the autoantibodies appeared to develop after the initiation of methimazole therapy and disappeared again after the cessation of methimazole. this binding activity to labelled t4 analogue was demonstrated to be in the immunoglobulin g with a kappa l ... | 1990 | 2202173 |
| a comparison of triiodothyronine and thyroxine in the potentiation of tricyclic antidepressants. | several studies suggest that small amounts of triiodothyronine (t3) will convert tricyclic antidepressant nonresponders into responders within a few days to a few weeks. as thyroxine (t4) is converted to t3 to have its physiological effect, it has been assumed that t4 would have a similar antidepressant effect to that of t3. we carried out a randomized, double-blind evaluation of the antidepressant-potentiating effect of t3 as compared with t4. significantly more patients responded to 3 weeks of ... | 1990 | 2201988 |
| changes in thyroid hormone and insulin status of the brown adipose tissue of cold acclimated rats on short term exposure to heat. | acclimation of rats to cold caused 45% increase in the concentration of triidothyronine (t3) and 35% increase in the concentration of thyroxine (t4) in serum. exposure of cold-acclimated rats to heat (12 hr, 37 degrees c) failed to decrease the concentrations of thyroid hormones in circulation. the concentration of t3 in brown adipose tissue (bat) increased almost 10-fold on cold acclimation. iodothyronine deiodinase activity also registered 3-fold increase. exposure of cold-acclimated animals t ... | 1990 | 2201623 |
| atypical subependymoma of the spinal cord: ultrastructural and immunohistochemical studies. | a 76-year-old woman with a 2-year history of progressive weakness of the left arm and leg underwent a laminectomy for an intradural, extramedullary, pedunculated subependymoma involving the meninges and spinal nerve roots at c7-t1. eight months later, another operation was necessary for an intramedullary neoplasm at c3-t4. this latter tumor consisted of small, poorly developed cells, large atypical astrocytes, and ependymal cells. neither ependymal rosettes nor papillary formations were present. ... | 1990 | 2200977 |
| systematic evolution of ligands by exponential enrichment: rna ligands to bacteriophage t4 dna polymerase. | high-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. in particular one eight-base region of an rna that interacts with the t4 dna polymerase was chosen and randomized. two different sequences were selected by this pr ... | 1990 | 2200121 |
| a randomized trial comparing adjuvant fluorouracil, doxorubicin, and mitomycin with no treatment in operable gastric cancer. international collaborative cancer group. | three hundred fifteen patients with operable gastric cancer were randomized to receive fluorouracil, adriamycin (doxorubicin; adria laboratories, columbus, oh), and mitomycin (fam) or no adjuvant treatment between september 1981 and july 1984. after excluding ineligible patients, 281 patients are included in this analysis. treatment was moderately well tolerated by the majority of patients, the common side effects being nausea and vomiting (58%) and alopecia (57%). three possible treatment-relat ... | 1990 | 2199622 |
| resolution of holliday junction analogs by t4 endonuclease vii can be directed by substrate structure. | endonuclease vii is an enzyme from bacteriophage t4 capable of resolving four-arm holliday junction intermediates in recombination. since natural holliday junctions have homologous (2-fold) sequence symmetry, they can branch migrate, creating a population of substrates that have the branch point at different sites. we have explored the substrate requirements of endonuclease vii by using immobile analogs of holliday junctions that lack this homology, thereby situating the branch point at a fixed ... | 1990 | 2199447 |
| escherichia coli rnase e has a role in the decay of bacteriophage t4 mrna. | bacteriophage t4 mrnas are markedly stabilized, both chemically and functionally, in an escherichia coli strain deficient in the rna-processing endonuclease rnase e. the functional stability of total t4 messages increased 6-fold; we were unable to detect a t4 message whose functional stability was not increased. there was a 4-fold increase in the chemical stability of total t4 rna. the degree of chemical stabilization of six specific t4 mrnas examined varied from a maximum of 28-fold to a minimu ... | 1990 | 2199322 |
| subcutaneous recombinant granulocyte-macrophage colony-stimulating factor used as a single agent and in an alternating regimen with azidothymidine in leukopenic patients with severe human immunodeficiency virus infection. | we investigated the effects of recombinant human granulocyte-macrophage colony-stimulating factor (rgm-csf) administered by the subcutaneous route, first alone and then alternating with azidothymidine (azt), in leukopenic patients with severe human immunodeficiency virus (hiv) infection. ten patients with acquired immunodeficiency syndrome (aids) or related disorders, five of whom could not tolerate conventional doses of azt, were administered rgm-csf subcutaneously for 12 days. they then were a ... | 1990 | 2198957 |
| [liver and thyroid gland. physiopathologic and clinical relationships]. | the liver plays a dominant role in the metabolism of the thyroidal hormones; it is here that the 5' deiodase acts to convert part of t4 to t3. there are eight further circulating iodothyronines: the rt3, mainly derived from t4, appears to be the major inhibitor of t4 and t3. thus, if rt3 increases, the metabolic effects of t3 and t4 can be quite different. in the course of some chronic systemic diseases (e.g. hepatic cirrhosis) rt3 increases simultaneously with the decrease of t3 levels. therefo ... | 1990 | 2198647 |
| review: thyroid function in psychiatric illness. | the development of highly sensitive immunometric assays for thyroid-stimulating hormone (tsh) has provided increased understanding of thyroid hormone regulation but, paradoxically, has contributed to a kaleidoscopic complexity of thyroid function test variability in hospitalized patients with nonthyroidal illness (nti). in primary hypothyroidism, an elevated tsh is the most sensitive chemical index available, although early cases may show a hyperresponse of tsh to thyrotropin-releasing hormone ( ... | 1990 | 2198197 |
| spontaneous shuffling of domains between introns of phage t4. | the three self-splicing introns in phage t4 (in the td, suny and nrdb genes) (fig. 1a) each have the conserved group i catalytic rna core structure (fig. 1b), out of which is looped an open reading frame. although the core sequences are very similar (approximately 60% identity), the open reading frames seem to be unrelated. single crossover recombination events between homologous core sequences in the closely linked td and nrdb introns have led to 'exon shuffling. here we describe spontaneous do ... | 1990 | 2197562 |
| blunt-end and single-strand ligations by escherichia coli ligase: influence on an in vitro amplification scheme. | a ligase-based, in vitro dna amplification system (lar) has been described by wu and wallace [genomics 4 (1989) 560-569]. this strategy is based on the ability of a dna ligase to join the 5' phosphate of one dna molecule to the 3' hydroxyl of a second during a nick-closing reaction. escherichia coli dna ligase has been used in place of the t4 dna ligase in our study in order to limit template-independent ligation activities, which lower the sensitivity of this amplification procedure. the result ... | 1990 | 2197176 |
| [intrarectal ultrasonography in the preoperative assessment of the depth of infiltration of rectal tumors]. | from june 1987 to february 1990, 142 out of 319 patients with rectal tumours were investigated by ultrasound scanning with the object of ascertaining the depth to which the tumour had infiltrated. this examination proved completely feasible in 130 of these patients (84 men, 46 women, mean age 61.7 [42-84] years). postoperative histological examination revealed an adenoma (n = 59) or a t1 carcinoma (n = 19), while intrarectal ultrasonography disclosed the same findings in 79 patients (true-positi ... | 1990 | 2197069 |
| sequencing of saccharomyces telomeres cloned using t4 dna polymerase reveals two domains. | by using t4 dna polymerase rather than s1 or bal31 nuclease to clone yeast telomeres, very little telomeric dna is lost. these clones were used to determine the dna sequence of virtually the entire telomeric tract. our results demonstrated that a slightly modified version, c2-3a(ca)1-6, of the consensus derived from sequence analysis of more-internal regions (j. shampay, j. w. szostak, and e. h. blackburn, nature [london] 310:154-157, 1984) extends to the very end of the chromosome. the sequence ... | 1990 | 2196453 |
| automated electrokinetic analysis; description and application in virology and cell biology. | the electrophoretic mobility (epm) of selected macromolecules in solution was shown to be accurately determined using an automated electrokinetic analyzer, the penkem s3000. in addition, the s3000 was used to monitor the effects of t4 phage infection on the epm of escherichia coli b. epm, expressed as the ratio of velocity in microns/sec to field strength in v/cm, was measured for calf thymus dna, for pneumococcal capsular polysaccharide serotype 3 (pcp-3), and for bovine serum albumin (bsa) unb ... | 1990 | 2196274 |
| overexpression, purification, and characterization of recombinant t4 gene 32 protein22-301 (g32p-b). | gene 32 protein (g32p), the replication accessory protein from bacteriophage t4, is a zinc metalloprotein which binds with high cooperativity to single-stranded (ss) nucleic acids. the basic n-terminal 21 amino acids (termed the "b" domain) is required for highly cooperative (omega approximately 500) binding of g32p monomers to ss nucleic acids. as part of our studies to systematically evaluate the structural features of the b domain important for cooperative binding, a homogeneous source of g32 ... | 1990 | 2195020 |
| efficient site directed in vitro mutagenesis using ampicillin selection. | a novel plasmid vector pselect-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. the mutagenesis method is based on the use of single-stranded dna and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampicillin resistance. using t4 dna polymerase and t4 dna ligase the two primers are physically linked on the template. the non-mutant dna strand ... | 1990 | 2194159 |