Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| participation of tri-iodothyronine and metabolic clearance rate in the inhibition of growth hormone secretion in thyroxine-treated domestic fowl. | surgical thyroidectomy increases basal and trh-induced gh concentrations in the peripheral plasma of immature domestic fowl. replacement therapy with thyroxine (t4; 100 micrograms/kg per day for 7 days, i.m.) suppressed the gh responses to thyroidectomy. bolus administration of t4 (10 micrograms/kg, i.m.) to thyroidectomized birds promptly lowered the circulating gh concentrations, which remained suppressed for at least 4 h. chronic (daily injections for 7 days) or acute (one injection) pretreat ... | 1990 | 2313216 |
| computed tomography of the clinically negative neck. | members of the new york head and neck society conducted a multi-institutional review correlating preoperative computed tomography (ct) of the neck with postoperative pathology in 59 patients with squamous cell carcinoma of the oral cavity, pharynx, and larynx, without palpable lymphadenopathy. all underwent ct followed by surgery that included partial or complete cervical lymphadenectomy. sixteen (28%) patients had occult cervical metastases including 6 (17%) of 36 patients with "early stage" (t ... | 1990 | 2312275 |
| mutational analysis of the phage t4 morphogenetic 31 gene, whose product interacts with the escherichia coli groel protein. | the phage t4 morphogenetic gene 31 has been sequenced. its deduced gene product is a polypeptide of 111 aa, with a predicted mr of 12064 and a pi of 4.88. the proof that the assigned open reading frame (orf) encodes gp31 rests on the sequencing of two known gene 31 amber mutations, amn54 and ng71, demonstrating that these mutations result in translational termination within the assigned orf. furthermore, the sequencing of four different t4 epsilon mutations, isolated on the basis of allowing the ... | 1990 | 2311934 |
| planar vector projection of short-latency median nerve somatosensory evoked potentials. | a planar vector projection of short-latency somatosensory evoked potentials (sep) following stimulation of the median nerve was obtained by recording sep over fpz-oz and t3-t4 and plotting the amplitudes on both channels at corresponding time points against each other. the resulting curve showed three successive loops pointing ipsilateral occipital (n1), contralateral occipital (n2) and ipsilateral frontal (n3) to the stimulated side. normal values for latency, amplitude and direction of these l ... | 1990 | 2311569 |
| influence of catecholamines, prostaglandins and thyroid hormones on growth hormone secretion by chicken pituitary cells in vitro. | in young chickens plasma concentrations of growth hormone (gh) are depressed by prostaglandins (pg) e1 and e2, epinephrine, norepinephrine, alpha 2 and beta agonists or thyroid hormones. a primary culture of chicken adenohypophyseal cells was used to examine the direct effects of these agents at the level of the pituitary as evaluated by gh release in the presence and absence of growth hormone releasing factor (grf). following collagenase dispersion and culture (preincubation, 48 hr) cells were ... | 1990 | 2311372 |
| radioimmunological determination of total thyroxin by antibodies immobilized on polystyrene tubes coated with styrene-maleic anhydride copolymers. | in this new technique for preparing activated polystyrene tubes for assay of human thyroxin (t4) we coated plastic with styrene-maleic anhydride copolymers. the quantity of antibodies immobilized on these tubes was considerably greater than on non-activated polystyrene tubes, and the time required for immobilization was shortened. calibration curves were plotted, and determination of total t4 in 200 serum samples confirmed the expected increase in sensitivity as compared with a commercial (gamma ... | 1990 | 2311225 |
| concentrations of free thyroxin and free triiodothyronine in serum of patients with thyroxin- and triiodothyronine-binding autoantibodies. | between 1982 and 1989 we identified 47 subjects with spuriously increased concentrations of free thyroxin (ft4) or free triiodothyronine (ft3) related to autoantibody interference in analog ft4 and (or) ft3 methods. the incidence of autoantibody interference observed during one year (1988) was 1 in 2460. in the subjects identified, 51% and 11%, respectively, showed an increased binding of radiolabeled t4 or t3 analog alone; 38% had an increased binding of both. of 36 patients tested, 71% had aut ... | 1990 | 2311215 |
| edrophonium priming for antagonism of atracurium neuromuscular blockade. | edrophonium administered in divided doses has been reported to accelerate antagonism of neuromuscular blockade, i.e., a "priming" effect. since measured onset times can be affected by the type of stimulation used, this effect was studied using both train-of-four (tof) and single twitch (st) stimulation. during thiopentone-nitrous oxide-enflurane anaesthesia 20 adults were given atracurium 0.5 mg.kg-1. both ulnar nerves were stimulated with tof every 12 sec until one per cent recovery of first tw ... | 1990 | 2311149 |
| survival rates in patients with differentiated thyroid carcinoma. influence of postoperative external radiotherapy. | nine hundred thirty-two patients with papillary and follicular thyroid carcinomas were seen at the departments of medicine, surgery, and radiology of the university of essen, essen, germany, between 1970 and 1986. in addition to standard treatment by surgery, radioactive iodine and medical thyroid stimulating hormone (tsh) suppression, 346 patients had received conventional external irradiation to the neck (mostly 40-60 gy) before referral to our institutions, whereas 586 patients had not receiv ... | 1990 | 2311063 |
| hemorrhagic sequelae of immune thrombocytopenic purpura in human immunodeficiency virus-infected hemophiliacs. | clinical bleeding tendency and tests of immune function were studied prospectively in 11 human immunodeficiency virus (hiv)-infected hemophiliacs with immune thrombocytopenic purpura (itp) and a platelet count less than 50,000/microl. these 11 patients represented 13% of a well-characterized cohort of 87 hiv + hemophiliacs. itp developed a mean 3.5 years after seroconversion, mean platelet count at presentation was 36,000/microl (range 15,000 to 49,000/microl), and the mean age at seroconversion ... | 1990 | 2310826 |
| increased production of thyroxine and inappropriately elevated serum thyrotropin in levels in endogenous depression. | the increased serum thyroxine (t4) levels in endogenous depression (ed) might be due to an increased production or a reduced degradation of t4. we therefore performed turnover studies of radiolabeled t4 and 3,5,3'-triiodothyronine (t3) in 6 patients with ed and 8 age-matched healthy controls. in ed, the median daily production of t4 was 130 nmol/day/70 kg, (range 100-186 nmol/day/70 kg) and elevated compared to control values which were 99 nmol/day/70 kg (range 85-142 nmol/day/70 kg) (p less tha ... | 1990 | 2310802 |
| a refined calculation of the solution dimensions of the complex between gene 32 protein and single stranded dna based on estimates of the bending persistence length. | the rotation diffusion coefficient of a complex of gp32, the single stranded dna binding protein of the bacteriophage t4, with a single stranded dna fragment with about 270 bases was determined to obtain further information on the flexibility of this particle. the rotation diffusion of these molecules is used as a sensitive measure of the flexibility of different dna protein complexes. using the theory of hagerman and zimm (biopolymers 20, 1481 (1981)) and assuming a bending persistence length o ... | 1990 | 2310524 |
| solution structure of bacteriophage t4d and icosahedral capsid geometry visualized in freeze-fractured, deep-etched replicas. | the prolate icosahedral capsid geometry of wild type bacteriophage t4d has been determined by direct visualization of the triangular faces in stereoimages of transmission electron micrographs of phage particles. bacteriophage t4 was prepared for transmission electron microscopy (tem) following a protocol of freeze-fracturing, deep-etching (fdet) and replication by vertical deposition (80 degrees angle) of a thin platinum-carbon (pt-c) metal layer of 1.01 nm. from direct statistical measurements ... | 1990 | 2310514 |
| thyroid function and thyroxine metabolism in hexachlorobenzene-induced porphyria. | the effects of hexachlorobenzene (hcb) administration on the development of porphyria and on changes in thyroid function and thyroid hormone metabolism were examined. female wistar rats were treated with hcb for 1 or 8 weeks. at both treatment times liver weight was notably increased with a slight change in thyroid weight at 8 weeks. serum thyroxine (t4) levels were depressed, whereas levels of triiodothyronine (t3) were not depressed significantly at both treatment times. one or eight weeks of ... | 1990 | 2310407 |
| fatal nonthyroidal illness may impair nocturnal thyrotropin levels. | this study was undertaken to assess whether circadian variation of thyrotropin (tsh) is affected by the severity of a nonthyroidal illness. | 1990 | 2309741 |
| b to z transitions of the short dna hairpins formed from the oligomer sequences: d[(cg)3x4(cg)3] (x = a, t, g, c). | circular dichroism (cd) spectra were collected as a function of sodium perchlorate concentration [naclo4] for the set of dna hairpins formed from the oligomer sequences d[(cg)3x4(cg)3] where x = a, t, g or c. over the range in salt concentration from 0 to 4.0 m naclo4, the cd spectra invert in a manner characteristic of the b to z transition. a factor analysis routine is described and employed to determine the least number of basis spectra required to fit the measured spectra of each hairpin ove ... | 1990 | 2308846 |
| [a case of aorto-coronary bypass surgery in a hypophysectomized male patient]. | a 53-year-old hypophysectomized male patient who had been on adjuvant therapy with cortizol and thyroxin, underwent aorto-coronary bypass surgery for angina pectoris with 3 vessel obstruction. following revascularization surgery, he had an uneventful course until 8th post-operative day, when he experienced about of unconsciousness which was relieved by intravenous hydrocortizon supplement. simultaneously complicated bradycardia and peripheral coldness were considered to be due to lowered serum t ... | 1990 | 2308247 |
| [influence of the anti-t4 autoantibody cross-reacting with t3 on serum t3 level determined by ria]. | we encountered a patient who showed remarkably high binding capacities of both 125i-t4 and 125i-t3, which suggests the presence of antithyroid hormone autoantibody. however, the serum level of t3 in the patient was not abnormal. study of the specificity of anti-thyroid autoantibody revealed that the antibody present in the patient was the anti t4 antibody, which cross-reacted with t3. in addition, the binding capacity of the t4 autoantibody with t3 was inhibited by t4. endogenous t4 is always pr ... | 1990 | 2308227 |
| deletion-tolerance and trans-splicing of the bacteriophage t4 td intron. analysis of the p6-l6a region. | non-directed mutagenesis and phylogenetic comparison suggest that certain elements of the bacteriophage t4 td group ia intron are dispensable to self-splicing. the l6-p6a-l6a region was identified as a potential non-essential element, and was removed by sequential deletions extending from the l6a loop toward the p6 pairing. assays for splicing indicate that as long as the p6 pairing is maintained, the 1016 nucleotide td intron can be reduced to less than 250 nucleotides while maintaining functio ... | 1990 | 2308166 |
| chronic mucocutaneous candidiasis in childhood and complications of non-candida infection: a report of the pediatric immunodeficiency collaborative study group. | we reviewed the clinical course in 43 patients from eight medical centers who were given the diagnosis of chronic mucocutaneous candidiasis, a rare disorder of unknown cause that may occur in childhood. recurrent or severe infections with organisms other than candida were seen in 80% of the patients. there were nine cases of septicemia. seven patients have died; six of these deaths were directly related to non-candida infectious complications. endocrine dysfunction, including addison disease (11 ... | 1990 | 2308026 |
| erythrocyte zinc concentration in patients with subacute thyroiditis. | we have recently reported that red blood cell (rbc) zinc (zn) in patients with hyperthyroidism reflects a patient's integrated thyroid hormone level over the previous few months. in the present paper rbc zn concentrations were measured in 10 patients with subacute thyroiditis whose total plasma t4 and t3 levels were elevated. the values were compared with those obtained in 10 patients with untreated graves' disease, whose plasma t4 concentrations were elevated to the same level as in the former ... | 1990 | 2307731 |
| prevalence of antithyroglobulin antibodies detected by enzyme-linked immunosorbent assay of canine serum. | antithyroglobulin antibody (ata) values were higher in dogs with low total serum thyroxine (t4) and triiodothyronine (t3) values than in dogs with t3 and t4 values within reference value limits. in the population studied, doberman pinschers were predisposed to the development of ata; there was no sex predilection for development of ata. antithyroglobulin antibodies and thyroid status were evaluated in 2 groups of healthy dogs (n = 30) and in 470 canine serum samples submitted for t3 and t4 value ... | 1990 | 2307612 |
| genetic evidence for two protein domains and a potential new activity in bacteriophage t4 dna polymerase. | intragenic complementation was detected within the bacteriophage t4 dna polymerase gene. complementation was observed between specific amino (n)-terminal, temperature-sensitive (ts) mutator mutants and more carboxy (c)-terminal mutants lacking dna polymerase polymerizing functions. protein sequences surrounding n-terminal mutation sites are similar to sequences found in escherichia coli ribonuclease h (rnase h) and in the 5'----3' exonuclease domain of e. coli dna polymerase i. these observation ... | 1990 | 2307357 |
| ontogeny of the effect of purified chicken growth hormone on the liver 5' monodeiodination activity in the chicken: reversal of the activity after hatching. | the ontogeny of the effect of chicken growth hormone (c-gh) on the liver 5' monodeiodination (5'-d) activity was studied in chickens starting from 14-day-old embryos until 5-week-old chickens. ten micrograms of the purified hormone was injected intravenously and after 2 hr, blood and liver samples were taken. in all embryonic stages tested, c-gh stimulated the peripheral thyroxine (t4) to triiodothyronine (t3) conversion as demonstrated by the increased plasma t3 level and liver 5'-d activity wh ... | 1990 | 2307344 |
| [an unusual association of transient resolving thyrotoxicosis due to painless thyroiditis, hypopituitarism and central diabetes insipidus associated with spontaneous pituitary apoplexy]. | pituitary apoplexy is characterized by a wide spectrum of clinical features. a quite rare case of painless thyroiditis, hypopituitarism and central diabetes insipidus (di) followed by pituitary apoplexy was presented. a 61-year-old woman was admitted to our hospital in may, 1986 because of marked general malaise, polydipsia and weight loss which became progressively worse. four months earlier she had experienced episodes of abrupt onset of severe headache associated with nausea and blurring visi ... | 1990 | 2307257 |
| 5'-deiodination in rat hepatocytes: effects of specific flavonoid inhibitors. | 5'-deiodination of t4 or rt3 in the presence of flavonoids was studied in freshly isolated suspended rat hepatocytes. flavonoids, a novel group of synthetic deiodinase inhibitors, were designed to act as t4 antagonists. 5'-deiodination of the prohormone t4 to the thyromimetically active t3 is an essential first step in controlling thyroid hormone action. hepatocytes were incubated with either 2 microm t4 or 2 microm [125i]rt3 as 5'-deiodinase substrates in the absence and presence of inhibitors ... | 1990 | 2307124 |
| growth hormone aggregates in the rat adenohypophysis. | although it has been known for some time that gh aggregates are contained within the rat anterior pituitary gland, the role that they might play in pituitary function is unknown. the present study examines this issue using the technique of western blotting, which permitted visualization of 11 gh variants with apparent mol wt ranging from 14-88k. electroelution of the higher mol wt variants from gels followed by their chemical reduction with beta-mercaptoethanol increased gh immunoassayability by ... | 1990 | 2307122 |
| the hiv protein, gp120, activates nuclear protein kinase c in nuclei from lymphocytes and brain. | nuclear pool(s) of protein kinase c (pkc) may be a common target for hormones and growth factors which affect the trophic state of cells. the data presented demonstrate a time and dose-dependent activation of nuclear pkc by the hiv coat protein, gp120, in isolated nuclei from rat spleen and hippocampus. this gp120-stimulated pkc response was blocked by specific pkc inhibitors, a monoclonal antibody to pkc, and a monoclonal antibody directed against the murine t4 analog, l3t4. it is suggested tha ... | 1990 | 2306233 |
| quantized viral dna packaging revealed by rotating gel electrophoresis. | two classes of missense mutations in the bacteriophage t4 gene coding for the major head protein produce phage with different length heads. the pt (petite) mutations produce phage with normal, intermediate, and isometric heads, whereas ptg (petite and giant) mutations also produce greatly elongated (giant) heads. dna from petite, normal, and giant particles was clearly resolved by discontinuous rotating gel electrophoresis, and several new species of headful length dna were found. these results ... | 1990 | 2305552 |
| in vitro replication through nucleosomes without histone displacement. | a well-characterized set of proteins encoded by bacteriophage t4 replicates dna in vitro and generates replication forks that can pass nucleosomes. the histone octamers remain associated with newly replicated dna even in the presence of excess dna competitor, and intact nucleosomes re-form on the two daughter dna helices. it is concluded that nucleosomes are designed to open up transiently to allow the passage of a replication fork without histone displacement. | 1990 | 2304549 |
| [long-term follow-up study of the surgical treatment of pulmonary adenocarcinoma]. | the surgical treatment of pulmonary adenocarcinoma was studied on the basis of the postoperative long-term results in 211 cases. the overall five-year survival rate was 42% for adenocarcinoma and 48% for squamous cell carcinoma, and there was no significant difference. in stage i, as the degree of differentiation became lower, the prognosis became poorer. in stage iii, the five-year survival rate was 50% for the alveolar cell type and 32% for the well differentiated type. the prognosis was bette ... | 1990 | 2304294 |
| stimulation of adult chondrocyte metabolism by a thyroid-derived factor. | this paper reports the effects of adding partially purified bovine thyroid calcitonin, thyrocalcitonin, to adult bovine articular cartilage cells. thyrocalcitonin stimulated chondrocyte proliferation fourfold under low serum (0.5%) culture conditions. in serum-free medium, thyrocalcitonin stimulated cell proliferation more than twofold. with high-density cultures in serum-free medium, chondrocyte glycosaminoglycan (gag) synthesis was stimulated 60% by thyrocalcitonin. cell-associated radioactivi ... | 1990 | 2303955 |
| catecholamine and thyroid hormones in traumatic injury. | during hyperthyroidism, hypothyroidism, and severe stress there is often an inverse relationship between plasma norepinephrine (ne) and thyroid hormones. we evaluated this relationship in patients who were severely burned, patients who had injury to both brain and body, patients with head injury, and patients receiving high dose barbiturates for head injury. head-injured patients had a low thyroxine (t4), low triiodothyronine (t3), and high reverse t3. phenytoin for control of seizures lowered t ... | 1990 | 2302947 |
| t3 augmentation of a tricyclic antidepressant in a patient receiving t4 maintenance therapy. | 1990 | 2301670 | |
| rapid analysis of lymphocyte subsets in cord blood. | several investigators have enumerated cellular populations in neonatal cord blood with variable results. in this study, the authors established reference ranges for lymphocyte subsets in cord blood from healthy newborns using a whole blood lysis technique on the coulter immunoprep epics leukocyte preparation system (coulter immunology, hialeah, fl). all analyses were performed on a flow cytometer by gating on forward angle versus 90 degrees light scatter. lymphocytes demonstrated all surface mar ... | 1990 | 2301287 |
| persistent lymphadenopathies in people at high risk for hiv infection. clinicopathologic correlations and long-term follow-up in 79 cases. | clinicopathologic correlations were explored in 79 patients at high risk for human immunodeficiency virus (hiv) infection who had lymph node biopsy for persistent lymphadenopathies and were followed for intervals of up to 7.2 years. three histologic patterns, follicular hyperplasia with cytolysis (a), follicular involution with hypervascularity (c), and a combination of the previous two (b), were recognized. ninety lymph node biopsies (79 primary and 11 sequential) were classified into the three ... | 1990 | 2301283 |
| therapeutic potential of two over-the-counter thyroid hormone preparations. | various brands of over-the counter (otc) exogenous thyroid hormones are available in health food stores and retail pharmacies. two commercially available otc thyroid products were analyzed for total thyroxine (t4) and triiodothyronine (t3) content. the strength of a liquid thyroid gland extract was unlabeled and that of the solid oral preparation was 45 mg per table. the t4 concentration of the liquid preparation was less than 10 micrograms/dl; that of t3 was below the analytical sensitivity of ... | 1990 | 2301185 |
| [results of radiotherapy of carcinomas of the tonsillar area. study of 137 cases]. | in our center, carcinomas of the tonsillar region are most often treated by radiotherapy. the aim of this retrospective study was to assess the therapeutical results obtained for such tumors over a period of ten years. from 1976 to 1986, 137 patients with carcinoma of the tonsillar area were exclusively treated by radiotherapy. the mean age of the patients was 54.3 years. 120 male and 17 female patients were included in the study. 63% of the patients had t3 or t4 tumors, while 53% had n2 or n3 a ... | 1990 | 2300785 |
| [modes of contamination in children]. | the various ways in which infants may be contaminated with hiv are described. transmission by blood perfusion persists in only some countries where hiv detection in blood products is not performed and sterilization is doubtful. transplacental contamination is well documented by the finding of hiv in various foetal tissues, amniotic fluid, placenta and foetal blood; it seems to be predominant. transmission during delivery and breast-feeding plays a lesser role, but it cannot be excluded. horizont ... | 1990 | 2300766 |
| [treatment of invasive epithelioma of the uterine cervix: the experience at the institut gustave-roussy]. | the therapeutic protocole used at the gustave roussy institute for invasive epithelioma of the uterine cervix rests, for limited forms (t1b-t2 proximal), on combined radiology and surgery. this include the recently introduced supraradical lymphadenectomy extended to the lumboarotic lymph nodes, first-line surgery in young women to preserve the ovarian function and postoperative chemotherapy for extra-pelvic n+ forms. in extensive forms (distal t2 and t3), the treatment of choice still is radioth ... | 1990 | 2300759 |
| erythrocyte zinc in hyperthyroidism: reflection of integrated thyroid hormone levels over the previous few months. | red blood cell (rbc) zinc (zn) concentration was measured by atomic absorption spectrophotometry in 28 healthy volunteers, in 46 patients with hyperthyroidism, and in 6 patients with hypothyroidism. the mean (+/- sd) rbc zn concentration in euthyroid controls was 11.4 +/- 1.5 mg/l rbc, and the normal range defined as the mean +/- 2 sd was 8.5 to 14.3 mg/l rbc. the mean rbc zn in patients with hyperthyroidism was decreased to 6.4 +/- 1.6 mg/l rbc, and 43 (93%) had low values. the mean rbc zn in p ... | 1990 | 2299989 |
| effect of iopanoic acid on basal and thyrotropin-stimulated thyroid hormone levels in suckling rat pups. | we proposed that basal and thyrotropin (tsh)-stimulated thyroid hormone levels of rat pups would be altered in the presence of iopanoic acid (ia), a radiographic contrast agent which competitively inhibits t4-to-t3 conversion, and that the nature of these changes would further depend upon the route of tsh administration in a manner distinct from that reported in adults. to test this hypothesis, litters from 24 sprague-dawley female rats were adjusted to 8 pups each. on day 5, 80 pups received ia ... | 1990 | 2299967 |
| structure of a t4 hairpin loop on a z-dna stem and comparison with a-rna and b-dna loops. | the synthetic dna oligomer c-g-c-g-c-g-t-t-t-t-c-g-c-g-c-g crystallizes as a z-dna hexamer, capped at one end by a t4 loop. the crystals are monoclinic, space group c2, with a = 57.18 a, b = 21.63 a, c = 36.40 a, beta = 95.22 degrees, and one hairpin molecule per asymmetric unit. the structure of the z-hexamer stem was determined by molecular replacement, and the t4 loop was positioned by difference map methods. the final r factor at 2.1 a resolution for hairpin plus 70 water molecules is 20% fo ... | 1990 | 2299669 |
| squamous cell carcinoma of the supraglottic larynx without clinically detectable lymph node metastases: problem of local relapse and influence of overall treatment time. | from january 1965 until december 1979, 203 patients with squamous cell carcinoma of the supraglottic larynx were treated with curative intent. the mean follow-up time was 10 years. the policy was to try to aim for cure by radiation therapy (rt) only, reserving surgery (s) for radiation therapy failures. for 193 patients the treatment consisted of a first series of radiation therapy to a total dose of 40 gy; if a good response to radiation therapy was obtained, the treatment was continued to a fu ... | 1990 | 2298616 |
| effect of biological alterations of type i 5'deiodinase activity on affinity labeled membrane proteins in rat liver and kidney. | type i iodothyronine 5'deiodinase (5'd-i) is a membrane-bound enzyme catalyzing the deiodination of t4 to t3. the affinity label, n-bromoacetyl-thyroxine (bract4), has previously been used to characterize a 27 kilodalton protein (p27) from rat liver and kidney microsomes with characteristics of the catalytic subunit of the 5'd-i. we examined the effect of physiological conditions, known to alter 5'd-i activity, on affinity-labeled proteins in rat liver and kidney microsomes. to confirm that the ... | 1990 | 2298172 |
| serum thyroid hormone changes in head and neck cancer patients treated with microwave hyperthermia on lymph node metastasis. | eleven patients with head and neck cancer (ten men, one woman; mean age, 65 years) (larynx, six; oropharynx, two; tongue, one; skin, one; thyroid, one) with regional lymph node enlargements either in contiguity or firmly adherent to the vascular structures of the neck have been treated by means of external microwaves (915, 434 mhz) applicators. all patients were treated by hyperthermia (42-45 degrees c) alone (ten sessions, twice a week, each lasting 30 minutes). thyroid hormones (t4, t3, ft4, f ... | 1990 | 2297661 |
| induction of type ii t4-5'-monodeiodinase activity in brown adipose tissue in fasted mice. | in order to study the effect of starvation on brown adipose tissue (bat) type ii 5'-monodeiodinating activity (5'mdi), type ii 5'mdi was measured in vitro in the presence of 20 mm dithiothreitol, 1 mm propylthiouracil, 2 nm thyroxine (t4) and appropriate amounts of 600 x g infranatant of bat from fed control or 3 day fasted mice, with or without daily t4 replacement (1.2 micrograms/100 g bw) during starvation. i- released from 125i-t4 was measured by ion-exchange column chromatography. activity ... | 1990 | 2296994 |
| thyroid regulation of resting metabolic rate and intermediary metabolic enzymes in a lizard (sceloporus occidentalis). | this study investigates the effects of physiological increments in plasma thyroxine (t4) at three levels of biological organization in thyroid-intact and thyroidectomized captive western fence lizards, sceloporus occidentalis. two doses of t4-loaded pellets elevated plasma t4 in thyroid-intact lizards from 4.8 +/- 0.47 to 10.7 +/- 2.25 and 20.4 +/- 5.77 ng/ml (mean +/- se). surgical thyroidectomy reduced t4 to 1.8 +/- 0.23 ng/ml, and subsequent t4 pellet implantation raised t4 to 14.8 +/- 4.30 n ... | 1990 | 2295423 |
| saturable triiodothyronine-binding sites in the pituitary nuclei of salmonid teleost fish. | high-affinity, limited-capacity, 3,5,3'-triiodo-l-thyronine (t3)-binding sites were established by in vitro saturation analysis in cell nuclei of the pituitary gland of arctic charr. the sites were extracted from the purified nuclei using 0.4 m nacl and incubated with [125i]t3 in the presence of 0.2 m nacl. t3 saturable binding attained equilibrium after 18-24 hr of incubation at 4 degrees. the association constant ranged from 6.7 to 20.1 liters.mol-1 x 10(9), indicating a t3 affinity greater th ... | 1990 | 2295420 |
| clinical significance of natural killer activity in patients with transitional cell carcinoma of the bladder. | we studied the relationship of natural killer activity from peripheral blood mononuclear cells with clinical stage of disease and the different modalities of treatment in 67 untreated patients with transitional cell carcinoma of the bladder and 29 normal controls. peripheral blood mononuclear cells from 39 patients with superficial bladder tumor (stages ta and t1) showed a natural killer cell activity similar to that of controls (p greater than 0.05), while in 28 patients with infiltrating tumor ... | 1990 | 2294256 |
| bone gla protein and sex hormone-binding globulin in nontoxic goiter: parameters for metabolic status at the tissue level. | several patients with nontoxic goiter have reduced serum tsh levels, as measured with new sensitive assays. whether this is a sign of subclinical hyperthyroidism, thus having the potential of adverse effects on different organs with time, is not known. we have measured serum levels of 2 markers of thyrometabolic status at the tissue level, bone gamma-carboxyglutamic acid-containing protein (bgp), reflecting the function of osteoblasts, and sex hormone-binding globulin (shbg), reflecting the func ... | 1990 | 2294139 |
| lack of nocturnal serum thyrotropin surge after surgery. | the effects of surgery on tsh secretion, with particular regard to the nocturnal tsh surge, were evaluated in 10 consecutive patients followed for 6 days after surgery. surgical trauma was associated in all patients with significant decreases in serum total and free t3 and a significant increase in serum rt3 levels, with no variations in serum total and free t4 concentrations. a marked increase in serum cortisol levels was observed, with higher values at night than in the morning. serum cortisol ... | 1990 | 2294138 |
| plasma norepinephrine kinetics, dopamine-beta-hydroxylase, and chromogranin-a, in hypothyroid patients before and following replacement therapy. | whether the increased plasma norepinephrine level reported in hypothyroidism is the result of impaired norepinephrine (ne) clearance or increased ne release by nerve terminals is unknown. we, therefore, measured plasma ne levels and clearance in 11 hypothyroid patients before [t4 index, 41.2 +/- 7.7 nmol/l (mean +/- sem); tsh, 71.4 +/- 23.0 mu/l] and 4 +/- 0.5 months after thyroid replacement (t4 index, 136.4 +/- 24.4 nmol/l; tsh, 3.2 +/- 1.2 mu/l) and in 8 healthy volunteers. plasma dopamine-be ... | 1990 | 2294136 |
| dynamics of 24-hour endogenous cortisol secretion and clearance in primary hypothyroidism assessed before and after partial thyroid hormone replacement. | although various abnormalities of hypothalamic pituitary adrenal function have been reported in primary hypothyroidism, neither 24-h patterns of pulsatile cortisol release nor estimation of its endogenous secretion and clearance rates have been fully investigated in this clinical setting. we studied pulsatile and circadian patterns of cortisol secretion in six hypothyroid men [mean free t4 index, 0.59 +/- 0.22 (+/- se); mean tsh, greater than 50 mu/l] by sampling blood at 20-min intervals for 24 ... | 1990 | 2294128 |
| alterations of human growth hormone binding by rat liver membranes during hypo- and hyperthyroidism. | hypothyroid rats treated with human gh (hgh) were partially refractory to the latter's effects. the present study was undertaken to investigate the role of hypo- and hyperthyroidism on the gh receptor. seven-week-old rats were rendered either hypothyroid, by methimazole, or hyperthyroid, by a daily overdose of t4, during weeks 7-14 of life. livers were homogenized and overlaid on sucrose discontinuous density gradient. removal of endogenous ligand from the receptor was performed by exposing the ... | 1990 | 2293991 |
| thyroidal regulation of rat pancreatic nuclear triiodothyronine receptor during postnatal development. | we have shown previously that the rat pancreas contains nuclear t3 receptors which exhibit a characteristic maturation pattern during development. to investigate whether these receptors are subjected to autologous regulation by thyroid hormones, the effect of t4 on the binding capacity (bmax), dissociation constant (kd), and receptor occupancy were followed in intact rat pups at various ages. hyperthyroidism (by daily injection of t4 0.1 micrograms/g body wt to intact pups starting 4 days before ... | 1990 | 2293985 |
| effects of 5,5'-diphenylhydantoin on thyroxine and 3,5,3'-triiodothyronine concentrations in several tissues of the rat. | we studied the effect of 5,5'-diphenylhydantoin (phenytoin, dph) on the metabolism of thyroid hormones, the intracellular concentration of t4, and the source and concentration of t3. two groups of six male wistar rats received a continuous infusion of 10 ml saline/rat. day. one group received dph in their food (50 mg/kg bw) for 20 days. for both groups [125i]t4 and [131i]t3 were added to the infusion fluid for the last 10 and 7 days, respectively. at isotopic equilibrium the rats were bled and p ... | 1990 | 2293983 |
| neuropsychiatric manifestations of altered thyroid state. | the authors assessed the mood and cognitive effects of sequential t4, t3, and withdrawal of thyroid hormone replacement on 25 patients who had had thyroidectomies for thyroid cancer. the patients experienced increased sadness and anxiety when they were without medication, but not significant difference in mood was noted between t4 and t3. the patients who experienced increased affective symptoms when not taking medication were more likely to have histories of affective illness or mood lability. | 1990 | 2293795 |
| different short-term effect of protein and carbohydrate intake on tsh, growth hormone (gh), insulin, c-peptide, and glucagon in humans. | the effect of isocaloric (500 kcal) protein and carbohydrate ingestion was studied in a crossover study in nine healthy humans. subjects were studied twice after overnight fasting, with an interval of 3 to 7 days. blood was collected for 240 min after food ingestion. the initial reaction of growth hormone (gh) and thyroid stimulating hormone (tsh) to protein and carbohydrate was identical, with a reduction in both gh and tsh, and nadir occurring after 45-60 min and 120 min, respectively. during ... | 1990 | 2293343 |
| hemodynamic response to low-dose epinephrine infusion during hypotensive epidural anesthesia for total hip replacement. | the hemodynamic response to reduction in blood pressure after epidural anesthesia in elderly patients is poorly defined. therefore, hemodynamic measurements using radial artery and thermodilution pulmonary artery catheters were performed in 85 patients undergoing total hip replacement in whom blood pressure was allowed to decrease in order to minimize blood loss. measurements were made in the lateral position prior to and after induction of epidural anesthesia to t4 or above when mean arterial p ... | 1990 | 2291884 |
| intracellular lytic enzyme systems and their use for disruption of escherichia coli. | this article focusses on lytic enzyme systems available in e. coli and their potential use for cellular disruption. in the systems described here the genetic information for lysis would be carried within the microbial host, either integrated or naturally occurring on chromosomal dna, or on extrachromosomal elements such as plasmids. each microbe would carry complete information for endogenous enzymatic lysis, and lysis would occur in a controlled manner after being triggered by an external facto ... | 1990 | 2291440 |
| neonatal skeletal maturation in congenital hypothyroidism and its prognostic value for psychomotor development at 3 years in patients treated early. | neonatal skeletal maturation was assessed by different methods based on the bone centres in the knee and ankle region in 46 infants with true-positive (patients) and 17 infants with false-positive screening tests (controls). the patients and controls did not differ in mean age at x-ray examination and age at the start of treatment (14.5 +/- 5.7 days). one of the methods used to score the size of femoral and tibial epiphyses was just as good as the other ones tested, but has the advantage of bein ... | 1990 | 2289785 |
| [influence of the season and of gestation on the plasma concentration of thyroid hormones: triiodothyronine (t3) and thyroxine (t4) in sahel peulh ewes]. | seasonal and gestational variations of triiodothyronine (t3) and thyroxine (t4) plasma concentrations were evaluated in 12 pregnant and 6 non pregnant adult sahel peulh ewes. blood samples were drawn from all animals at different dates for radio-immunoassay. in this particular breed of sheep, plasma triiodothyronine and thyroxine levels did not present significant variations from the beginning of the cool season (december) until the end of the dry warm season (may). on the contrary, a highly sig ... | 1990 | 2288455 |
| [nasopharyngeal carcinoma. an epidemiological and clinical study]. | clinical and epidemiological data from 46 cases of nasopharyngeal carcinoma retrieved from our e.n.t. department data base are analysed. these tumors represent about 5% of all head and neck carcinomas seen at our department between 1984 and 1989. neither geographic origin nor occupation of patients have proved significant to the epidemiological analysis. patients are most frequently in their sixth decade of life at presentation, with a clear male predominance, but not as marked as in other locat ... | 1990 | 2288399 |
| consideration on serum triiodothyronine (t3), thyroxine (t4) concentration and t3/t4 ratio in the patients of senile dementia--is it possible to prevent cerebro-vascular dementia? | serum t3 concentration and t3/t4 ratio in the patients of senile dementia (sd) are smaller than those in the healthy. especially, those in the group of cerebro-vascular dementia (cvd) were remarkably lower than the healthy. however average of serum t4 in the former is very similar to it in the latter. this fact suggests the disturbance of metabolic transformation of t4 to t3 in the post-apoplectic brain. it seems to lead man to the metabolic disturbances of glucose, protein, nucleic acid, etc, a ... | 1990 | 2288182 |
| nuclear dna analysis: the relevance of ploidy, dna heterogeneity and phases of the cell cycle in 329 patients with prostatic carcinoma. a study on a follow-up of eight years. | the ploidy, dna heterogeneity and the phases of the cell cycle of the tumor were analyzed, by means of single-cell dna cytophotometry, in 329 patients with locally advanced prostatic carcinoma to find out and establish prognostic factors apart from those already known (stage, grade). follow-up periods ranged from 1 to 9 years. 253 (76.8%) of the 329 patients had carcinoma stage t3 nx m0, and 76 of them (23.1%) had carcinoma stage t3/t4 n2-4 m1. 11.8% of the patients showed a cytological grade of ... | 1990 | 2288053 |
| the prevalence of subclinical hypothyroidism in adults with low-normal blood thyroxine levels. | the purpose of this study was to determine whether subclinical hypothyroidism, defined as the state in which there is an elevation of thyrotropin blood level (tsh) with a low-normal blood thyroxine level (t4), can be found in clinically healthy subjects. we identified, by screening of adults in an ambulatory patient care facility, 67 persons with t4 at the lower range of normal (4.5-6.0 micrograms/dl) who were free of acute nonthyroidal illness and who were not on medications known to affect t4, ... | 1990 | 2287498 |
| nucleotide sequence and control of transcription of the bacteriophage t4 mota regulatory gene. | a 2116bp segment of the bacteriophage t4 genome encompassing the mota regulatory gene has been sequenced. in addition to mota, five open reading frames were identified in the direction of early transcription. the mota gene encodes a basic protein of 211 amino acids with a predicted molecular weight of 23,559. measurements of the rate of transcription of mota showed that the promoter of this gene is turned off after only 2 min of t4 development. this early promoter presents a structure which is r ... | 1990 | 2287273 |
| human immunodeficiency virus infection and the intestine. | hiv is a retrovirus infecting cd4-positive cells causing profound immunosuppression, eventually clinically manifest as aids. the cells principally infected by hiv are t4 lymphocytes (helper) and macrophages. the eventual loss of helper cell function is the prime reason for immunodeficiency which renders the individual susceptible to opportunistic infections. hiv infection was first described in male homosexuals. however, the trend now is for seroprevalence to rise rapidly in intravenous drug abu ... | 1990 | 2285822 |
| [confirmation of the presence of a transcription termination site in a dna segment downstream from gene 31 of bacteriophage t4]. | two open reading frames followed by a rho-independent transcription terminator and early promoter were established in a sequenced region downstream from gene 31 of t4 phage. the mrna hairpin suggested by the terminator sequence has the structure (x)8cuucgg(y)8, the loop sequence uucg having been shown to significantly stabilize rna hairpins. | 1990 | 2285429 |
| [solid phase ligation of synthetic dna fragments]. | a synthetic oligodeoxyribonucleotide (oligo) covalently bound by an internucleotide linkage to the succinylated sephacryl s-500 support through 1.9-diaminononane spacer was used as starting compound to assemble the e. coli rec a promoter dna fragment from synthetic oligos by means of t4 dna ligase. the solid-phase assembly of the designed dna was performed by two ways: stepwise ligation of two pairs of oligos (2 dyads) or simultaneous ligation of four oligos (tetrad). both ways gave equal result ... | 1990 | 2285422 |
| on the usefulness of levothyroxine suppressive therapy in the medical treatment of benign solitary, solid or predominantly solid, thyroid nodules. | the efficacy of levothyroxine suppressive therapy in the treatment of benign solitary thyroid nodules is controversial. in order to investigate this issue further we studied 122 patients with a solitary, solid or predominantly solid, thyroid nodule. the benign (colloid) nature of all nodules was proved by fine-needle aspiration biopsy. at the pertechnetate-99m thyroid scanning 91% of the nodules were "cold" and 9% "warm". all the patients received suppressive oral doses of levothyroxine (0.1 to ... | 1990 | 2284885 |
| effect of polyamine depletion on dna damage and repair following uv irradiation of hela cells. | treatment of hela cells with the polyamine biosynthesis inhibitors, methylglyoxal bis(guanylhydrazone) (mgbg), difluoromethylornithine (dfmo) or a combination of the two, resulted in reduction in cellular polyamine levels. analysis of uv light-induced dna damage and repair in these polyamine depleted cells revealed distinct differences in the repair process relative to that seen in cells possessing a normal polyamine complement. initial yield of thymine dimers and rate of removal of these lesion ... | 1990 | 2284346 |
| prognosis for resected lung cancer patients with tumors greater than ten centimeters in diameter. | during the period 1962-1986, 43 lung cancer patients, 2.3% of the 1,832 patients who underwent pulmonary resections at the national cancer center hospital, tokyo, had tumors greater than 10 cm in diameter. these 43 cancers were classified postsurgically according to the 1987 guidelines for tnm classification of malignant tumors established by union internationale contre le cancer (uicc), and included 35 cases (81.0%) in stages iiia, iiib and iv. the histological tumor types were adenocarcinoma i ... | 1990 | 2283720 |
| comparison among 3 techniques of spinal and epidural anesthesia for caesarean section. | when comparing spinal block and epidural block groups, spinal block is simpler, easier and more reliable and it is useful for urgent caesarean sections because of its rapid onset. in the spinal group, 0.4 per cent heavy bupivacaine is more reliable than 0.5 per cent plain bupivacaine. spinal lidocaine can also be used for caesarean section requiring an operation time of not more than 1 hour. if the operation time is expected to be more than an hour, spinal block with 0.4 per cent heavy is prefer ... | 1990 | 2283488 |
| [n-acetylneuraminic acid (sialic acid) as a tumor marker in head and neck cancers]. | n-acetyl-neuraminic-acid (sialic acid) is a natural component of the cell surface. cancer cells show a higher concentration of sialic acid in the cell membrane, and the serum also shows an elevated level of sialic acid. the concentration of sialic acid was measured in the serum of 76 new tumour patients and 113 previously treated patients who were clinically free of recurrence. the primary tumour was classified as t1-t4 according to the uicc classification. a further group consisted of patients ... | 1990 | 2283329 |
| [ultrasound diagnosis of the epiglottis]. | in 1988 we described a method of diagnosis of the larynx using ultrasound, and called it echolaryngography. now we discuss the ultrasound diagnosis of the normal epiglottis function and lesions of the epiglottis. in principle, three methods of examination are possible with simultaneous b- and m-mode display: transverse function test, sagittal function test and diagonal function test of the epiglottis. transverse and sagittal transcutaneous access to the epiglottis is simple using a 7.5 mhz secto ... | 1990 | 2283328 |
| [studies on the factors affecting serum thyrotropin levels in healthy controls and on the thyroid function in depressed patients using a highly sensitive immunoassay]. | with a highly sensitive time-resolved fluorometric immunoassay (tr-fia), serum thyrotropin (tsh) levels were determined in various conditions in healthy subjects. in addition, we compared the thyroid function in 10 depressed female patients with that in 27 female controls. 1) we evaluated a highly sensitive time-resolved fluorometric immunoassay kit for serum tsh. the lower limit of detection of tsh in serum was 0.008 less than u/ml. the intraassay and interassay variances were 3.0 greater than ... | 1990 | 2282957 |
| bone changes in hyperthyroidism--a preliminary study. | thyrotoxicosis may cause osteopenia and lead to increased risk of fractures. from july 1988 to march 1989, 88 hyperthyroid patients and 65 age, sex matched normal controls were enrolled for lumbar spine bone mineral density (bmd) study. significantly lower bmd values can be found in premenopausal hyperthyroid women (1.233 gm/cm2 vs 1.317 gm/cm2, p less than p less than 0.05). in the group of thyrotoxicosis, no linear correlation can be found between bmd and the degree of hyperthyroidism (n = 84, ... | 1990 | 2282564 |
| outcome following radioactive iodine therapy in graves' disease. | one hundred and fifty three patients with graves' hyperthyroidism treated with i-131 from march 1984 to september 1988 were analyzed. the dose of i-131 was given according to the formula: 100 microci/gm x estimated thyroid size (gm) x 100/24 hr i-131 uptake (%) the mean dose was 5.41 mci and the maximum dose was 7 mci. after one year of follow up, 45 patients (29.4%) were euthyroid, 35 patients (22.9%) became hypothyroid, and 73 patients (47.7%) were persistently hyperthyroid. discriminant analy ... | 1990 | 2282562 |
| thyroid response to dexamethasone: a study on normal controls and patients with psychogenic sexual dysfunction. | pre- and postdexamethasone triiodothyronine (t3), thyroxine (t4), and tsh levels of thirteen patients with psychogenic sexual dysfunction and thirteen controls were studied. patients showed lowered t4 levels in comparison with the control group whereas t3 and tsh levels did not differ significantly. dexamethasone had a suppressive effect on tsh in patients and in controls while t3 levels were suppressed in the control group only. patients scored significantly higher on the hamilton depression sc ... | 1990 | 2281880 |
| [exclusive radiotherapy of locally advanced carcinoma of the larynx. analysis of the caseload of varese (1979-1986)]. | the authors analyse a retrospective series of 90 consecutive patients (pts) affected with locally advanced laryngeal carcinoma (t3-4, n0-3--tnm, uicc 1978) who were radically irradiated from november 1979 to december 1986 at the radiotherapy department of the general hospital of varese. all the patients were treated with 60co and two opposed parallel lateral fields and progressive shrinkage: 66 with conventional fractionation (2 gy once a day, 5 times a week), 24 with an accelerated hyperfractio ... | 1990 | 2281175 |
| changes in somatotropin and thyrotropin secretory patterns in aging rats. | in order to clarify whether pituitary enlargement influences the secretory patterns of growth hormone (gh) and thyrotropin (tsh) in old rats, we studied the correlation between pituitary weight and plasma levels of gh and tsh in sprague-dawley rats of different age and sex. young female (3-4 months; yf), old female (25 months; of), and senescent female (33-35 months; sf) rats and young male (3-4 months; ym) and old male (24-26 months; om) rats carrying chronic intraatrial cannulas were used. seq ... | 1990 | 2280806 |
| thyroid hormones during development of a marsupial, the tammar wallaby, macropus eugenii. | the levels of thyroid hormones in the plasma and the activities of 5'-deiodinase activity in liver and kidney were determined in the tammar wallaby, macropus eugenii, from early pouch life to adulthood. the total concentration of plasma thyroxine (t4) was below 15 nmol/l before day 75 of pouch life, rose to about 75 nmol/l at day 160, and then decreased to about 12 nmol/l in the adult. the total concentration of plasma tri-iodothyronine (t3) was below 0.4 nmol/l before day 120, increased to 3 nm ... | 1990 | 2280211 |
| direct effects of cytostatic therapy on the functional state of the thyroid gland and tbg in serum of patients. | this study analysed the concentrations of thyroid binding globulin (tbg) in the serum as well as the level of triiodothyronine (t3), thyroxine (t4), thyreostimulating hormone (tsh), thyroglobulin (tg) and t4/tbg coefficient, before therapy and on the fifth day of therapy in 27 patients of both sexes suffering from neoplasia. the patients were treated with cytostatic antibiotics, alkylating agents and podophyllines derivates, by protocol. serum t3 gains in concentration already after 5 days since ... | 1990 | 2279526 |
| selection of patients with breast cancer for routine follow-up bone scans. | routine radionuclide bone scans have not been considered cost-effective for the routine follow-up after treatment of primary breast cancer. however subgroups of patients exist in whom early relapse in the skeleton is likely and this study examines again the role of the bone scan in routine follow-up. serial radionuclide bone scans were performed every 6 months during the first 2 years of follow-up of 560 patients with breast cancer. tumor characteristics which predict early relapse in bone were ... | 1990 | 2278890 |
| cloning and sequencing of the endo-cellulase cdna from robillarda sp. y-20. | an endo-cellulase cdna has been screened from a robillarda sp. y-20 cdna expression library using polyclonal antibodies (immunoscreening). western blot analysis showed that recombinant cmcase i fused to beta-galactosidase with the molecular weight of approximately 150,000 was expressed in escherichia coli y1090. sequence analysis of the cloned cdna revealed that it consisted of 1,136 nucleotides. by comparison of the amino acid sequence deduced from the cdna and the n-terminal amino acid sequenc ... | 1990 | 2277031 |
| eeg power and coherence while male adults watch emotional video films. | quantitative eeg analysis recorded at f3, f4, t3, t4, p3, p4 was performed for a group of healthy right-handed male adults (n = 9) viewing video films varying in their inductiveness on the affective valence dimension. digital eog-correction permitted the inclusion of trials with eye movements. muscle artifacts were statistically treated by means of analysis of covariance (ancova). the configuration of topographically motivated eeg parameters corresponded to the subjective valence rating of diffe ... | 1990 | 2276946 |
| thermodynamic stability of the 5' dangling-ended dna hairpins formed from sequences 5'-(xy)2ggatac(t)4gtatcc-3', where x, y = a, t, g, c. | expressions for the partition function q (t) of dna hairpins are presented. calculations of q (t), in conjunction with our previously reported numerically exact algorithm [t. m. paner, m. amaratunga, m. j. doktycz, and a. s. benight (1990) biopolymers, 29, 1715-1734], yield a numerical method to evaluate the temperature dependence of the transition enthalpy, entropy, and free energy of a dna hairpin directly from its optical melting curve. no prior assumptions that the short hairpins melt in a t ... | 1990 | 2275982 |
| thyroxine binding to human serum albumin immobilized on sepharose and effects of nonprotein albumin-binding plasma constituents. | 125i-thyroxine (125i-t4) binding to human serum albumin (hsa) covalently attached onto cnbr-activated sepharose (hsa-sepharose) was studied. 125i-t4 binding to hsa-sepharose was rapid and saturable. nonlinear curve-fitting analysis of binding isotherms revealed two classes of binding sites. the values of dissociation constants of high and low affinity sites were 2.19 +/- 0.53 x 10(-6) m and 2.69 +/- 0.78 x 10(-5) m, respectively. the number of binding sites of the high and the low affinity sites ... | 1990 | 2275755 |
| [the effect of thyroparathyroidectomy, parathyroid hormone and calcitonin on plasma strontium in rats]. | the effect of thyroparathyroidectomy (tptx) on the plasmatic sr concentrations in rats previously supplemented with this element, has been studied, as well as its effect on the treatment of tptx rats with hormonal combinations and, finally, the one presenting hormonal excess or defect of the phosphocalcium metabolism regulating hormones: parathormone (pth) and calcitonin (ct). twenty four hours after tptx, the plasmatic sr concentrations show a pattern similar to those of ca and mg and contrary ... | 1990 | 2274697 |
| thyroid hormone regulates type i deiodinase messenger rna in rat liver. | conversion of the prohormone t4 to the active hormone t3 is catalyzed by 5'-deiodinases, enzymes that have not been purified. previous studies have shown that modulating thyroid status results in changes in type i deiodinase activity in the rat liver. we have quantitated type i deiodinase mrna in liver by an expression assay using xenopus laevis oocytes. we report here that changes in enzyme activity correlate closely with changes in levels of the mrna for this enzyme, indicating that thyroid ho ... | 1990 | 2274056 |
| specific induction of fibronectin gene in rat liver by thyroid hormone. | the regulation of fibronectin (fn) gene expression by thyroid hormone was studied. rats were rendered hypothyroid by thyroidectomy, and the administration of t4 or t3 was used to produce rats in various thyroid states. rna was extracted from fresh liver, kidney, and heart, and fn mrna was determined by dot blot hybridization with a 32p-labeled rat fn cdna probe. the specificity of the hybridization was assessed by northern blot analysis. in liver, thyroidectomy decreased the abundance of fn mrna ... | 1990 | 2274053 |
| structure of the beta-1,3-1,4-glucanase gene of bacillus macerans: homologies to other beta-glucanases. | the nucleotide sequence of an 852 base pair (bp) dna fragment containing the entire gene coding for thermostable beta-1,3-1,4-glucanase of bacillus macerans has been determined. the bglm gene comprises an open reading frame (orf) of 711 bp (237 codons) starting with atg at position 93 and extending to the translational stop codon taa at position 804. the deduced amino acid sequence of the mature protein shows 70% homology to published sequences of mesophilic beta-1,3-1,4-glucanases from b. subti ... | 1990 | 2274030 |
| isolation and genetic characterization of new uvsw alleles of bacteriophage t4. | the uvsw gene of bacteriophage t4 is required for wild-type levels of recombination, for normal survival and mutagenesis after uv irradiation, and for wild-type resistance to hydroxyurea. additionally, uvsw mutations restore the arrested dna synthesis caused by mutations in any of several genes that block secondary initiation (recombination-primed replication, the major mode of initiation at late times), but only partially restore the reduced burst size. a uvsw deletion mutation was constructed ... | 1990 | 2274029 |
| selection for lysis inhibition in bacteriophage. | for escherichia coli cells that have been infected by t-even bacteriophages (phages t2, t4, and t6), the adsorption of a second t-even phage results in an increase in the length of the original phage infection and an associated increase in the number of phages produced by the same infected cell. this is a phage encoded response called lysis inhibition. in this study the ecological significance of lysis inhibition is explored. in particular it is argued that lysis inhibition is an adaptive respon ... | 1990 | 2273898 |
| [the haptoglobin-streptococcus relationship]. | in many experiments beginning in 1977 prof. o. prokop and prof. w. köhler discovered that the human haptoglobins are able to agglutinate group g-streptococci, but the reaction depends on the haptoglobin type. haptoglobins of the types hp 2-2 and hp 2-1 are complete agglutinins and haptoglobin of the hp 1-1 type is incomplete ("blocking") antibody. later it was found that streptococci with the t4--antigen (from lancefield types a, c and g) are suitable for the test. | 1990 | 2273761 |
| growth hormone induced stimulation of the t4 to t3 conversion in fed and fasting dwarf goats. | fed and food deprived (7 days) adult dwarf goats were injected intravenously with 75 micrograms/kg b. w. of ovine growth hormone (o-gh). blood samples were collected from the jugular vein -2, -1 and 0 hr prior to and 30, 60, 90 and 120 min after injection and assayed for thyroxine (t4) and triiodothyronine (t3) concentrations. the 5'-monodeiodination (5'-d) activity was consequently determined in liver and kidney samples following slaughtering. fasting alone increased plasma concentrations of t3 ... | 1990 | 2272603 |
| influence of dietary lipid and carbohydrate levels and chronic 3,5,3'-triiodo-l-thyronine treatment on thyroid function in immature rainbow trout, oncorhynchus mykiss. | the influence of varying dietary levels of nonprotein energy sources (lipid, l; carbohydrate, c) and 3,5,3'-triiodo-l-thyronine (t3) on thyroid function in immature rainbow trout was studied. three diets of equivalent available energy content and identical nutrient composition, except for dissimilar concentrations of l and c (diet 1, l = 7%, c = 28.3%; diet 2, l = 13%, c = 14.9%; diet 3, l = 19%, c = 1.5%), were each supplemented with 0, 4, 8, or 12 ppm t3 and fed to satiation to trout at 6.5 +/ ... | 1990 | 2272475 |
| failure to relate thyroid hormones and in vitro 5'-monodeiodination activity to oocyte development and sex steroids in the giant swamp frog dicroglossus occipitalis at the equator. | females of the giant swamp frog dicroglossus occipitalis were captured in zaïre close to the equator in the course of 1 month. during this period, females with fully developed eggs were found, together with females of which the eggs were still in the first developmental stages. a close relationship was established between the maturation of the eggs and the studied gonadal factors: the gonadosomatic index, the oviduct weight, plasma estradiol-17 beta (e2) concentrations, plasma testosterone conce ... | 1990 | 2272468 |