Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses. | a panel of 30 monoclonal antibodies was defined and characterized with respect to the binding capacity in immunoperoxidase assay to different strains of pestivirus. using the panel it was possible to identify specifically all strains and isolates of hog cholera virus, hog cholera vaccines derived from 'c' strains, and most strains of bovine viral diarrhoea/border disease (bvd/bd) viruses (including those isolated from pigs). a small proportion of bvd/bd isolates from pigs and ruminants reacted o ... | 1991 | 1660637 |
| pestivirus antibodies in pigs in ireland. | 1991 | 1660192 | |
| pestivirus infections in norway. serological investigations in cattle, sheep and pigs. | serum samples from 1,133 dairy cows (187 herds), 3,712 ewes (103 flocks) and 1,317 adult pigs (877 herds), were tested for neutralizing antibodies against the nadl strain of bovine virus diarrhoea virus. the prevalence rate of seropositive animals was 18.5% in cattle, 4.5% in sheep and 2.2% in pigs, such seroreactors being found in 28% of the cattle herds and 18% of the sheep flocks. in all three species the rate showed considerable herd and geographical variation. in cattle the seroreactor rate ... | 1991 | 1659160 |
| specific sequence amplification of bovine virus diarrhea virus (bvdv) and hog cholera virus and sequencing of bvdv nucleic acid. | the pestiviruses are small enveloped rna viruses and are causative agents of economically important animal diseases in cattle, swine, sheep and goats worldwide. we used the polymerase chain reaction to amplify one common fragment of several different strains of both hog cholera virus and bovine virus diarrhea virus (bvdv). the fragment is located at the 5'-end of the genome immediately upstream of the open reading frame. this is a highly conserved region among the different published pestivirus ... | 1991 | 1659027 |
| detection of bovine viral diarrhea virus, using degenerate oligonucleotide primers and the polymerase chain reaction. | a technique for detection of bovine viral diarrhea virus (bvdv) from circulating blood leukocytes, using the polymerase chain reaction, is described. the published nucleotide sequences of 2 strains of bvdv and that of hog cholera virus were aligned and the information was used to design oligonucleotides coding for 2 regions of amino acid homology. the oligonucleotides were a mixed population including all possible codons for the conserved amino acids. these degenerate oligonucleotides were used ... | 1991 | 1656820 |
| hepatitis c virus: buoyant density of the factor viii-derived isolate in sucrose. | physicochemical and molecular characterization studies of hepatitis c virus (hcv), the major causative agent of parenterally transmitted non-a, non-b hepatitis (pt-nanbh), strongly suggest that it is a pesti-/flavivirus-like virus. additional studies show that the buoyant density of plasma-derived hcv in sucrose is significantly lower than that of most tissue culture-derived flaviviruses (1.20 g/cm3). our finding suggests, but does not prove, that at least one physicochemical property of hcv is ... | 1991 | 1655970 |
| [use of monoclonal antibodies for the differential diagnosis of pestivirus infections in swine]. | monoclonal antibodies (mab) specific for hog cholera virus (hcv), bovine viral diarrhoea virus (bvdv) or pestivirus were applied for the differential diagnosis of pestivirus infections in pigs. field virus isolated from 8 confirmed classical swine fever outbreaks and one suspect case was propagated in pk(15) cell cultures and identified by direct immunofluorescence (ifa) and peroxidase linked antibody (pla) assays. peroxidase-linked hcv, bvdv and pestivirus specific mab were applied in direct pl ... | 1991 | 1648800 |
| [a sensitive enzyme-linked immunosorbent assay (elisa) for the serological detection of antibodies against the european hog cholera virus]. | an elisa for the detection of antibodies against hog cholera virus (hcv) was developed. the hcv-specific glycoprotein gp53 served as diagnostic antigen after immobilization using a monoclonal capture antibody. due to the higher affinity of hcv-specific antibodies to the viral gp53, sera cross reacting with bovine viral diarrhea (bvd) virus were discriminated by the slope of the titration curves. | 1991 | 1646495 |
| [a new enzyme immunoassay for the detection of antibodies against the bovine viral diarrhea virus]. | an enzyme-linked immunosorbent assay (elisa), using the nonstructural protein p125/80 of the bovine viral diarrhoea (bvd) virus as antigen, was used for screening bvd-specific antibodies in 468 bovine sera. the results were compared with those obtained in a standard neutralization test (nt). both tests reacted positive with 457 sera. the elisa gave positive results with eight sera which were negative in the nt whereas one serum was positive in the nt and negative in the elisa. two sera could not ... | 1991 | 1646493 |
| a sequence comparison of the vp72 gene of african swine fever virus. | 1992 | 1633960 | |
| gel retardation analysis of ribonucleotide reductase gene expression in african swine fever virus. | 1992 | 1633957 | |
| swine leukocyte antigen and macrophage marker expression on both african swine fever virus-infected and non-infected primary porcine macrophage cultures. | swine leukocyte antigens (sla) and a macrophage specific marker were monitored on porcine macrophages cultured with or without macrophage colony stimulatory factor (m-csf) and on cells infected with african swine fever virus (asfv). sla expression was maximal either in the total cell extract or on the cell surface at 3-4 days of culture; after 4 days these values began to decrease. fluorescence analyses of immunostained macrophages cultured with or without m-csf indicated a major upward shift in ... | 1992 | 1632065 |
| an african swine fever virus gene with homology to dna ligases. | sequence analysis of the sali g region of the genome of a virulent isolate of asfv (malawi lil 20/1) has revealed an open reading frame with the potential to encode a 48 kilodalton (kd) polypeptide which has significant homology with eukaryotic and prokaryotic dna ligases. this asfv encoded gene also contains the putative active site region of dna ligases including the lysine residue which is necessary for enzyme-adenylate adduct formation, but lacks the c-terminal basic region conserved in othe ... | 1992 | 1614852 |
| morphogenesis of african swine fever virus in monkey kidney cells after reversible inhibition of replication by cycloheximide. | the late cytoplasmic phases of african swine fever virus (asfv) morphogenesis in monkey kidney cells have been studied by transmission electron microscopy, focusing attention on the synthesis of viral envelopes. morphogenesis was studied after reversible cycloheximide blockage of monkey kidney cells infected with asfv. asfv appears to synthesize its external and internal envelopes within the cellular cytoplasm, at the same time as the capsid is formed, with intracellular and extracellular virion ... | 1992 | 1605742 |
| cell culture propagation modifies the african swine fever virus replication phenotype in macrophages and generates viral subpopulations differing in protein p54. | we have detected 86 african swine fever (asf) virus-induced proteins in infected pig macrophages by two-dimensional electrophoresis. no differences among protein patterns of wild-type viruses could be observed by this methodology. however, during cell culture adaptation and propagation we have characterized changes in the molecular weight of the asf virus specified protein p54, which show direct correlation with both size and number of viral subpopulation variants generated during cell culture p ... | 1992 | 1604931 |
| characterization of p30, a highly antigenic membrane and secreted protein of african swine fever virus. | we have identified and characterized a 30-kda phosphoprotein (p30) of african swine fever virus (asfv) that is synthesized, membrane localized, and released into the culture medium at early times after infection. sequence analysis of the p30 open reading frame predicts a highly antigenic protein with putative phosphorylation, glycosylation, and membrane attachment sites. | 1992 | 1604821 |
| role of the host cell nucleus in the replication of african swine fever virus dna. | an examination by autoradiography of african swine fever virus-infected alveolar macrophages pulse labeled with [3h]thymidine showed that, at early times of viral dna replication, the grains were localized exclusively in the nucleus in 20% of the cells, while in 45% the label was found in the cytoplasm. in the remaining 35%, newly synthesized dna was detected in both the nucleus and the cytoplasm. at later times, the percentage of cells with grains in the nucleus decreased considerably. pulse-ch ... | 1992 | 1585638 |
| comparison of the sequence of the gene encoding african swine fever virus attachment protein p12 from field virus isolates and viruses passaged in tissue culture. | comparison of the amino acid sequence of the african swine fever virus attachment protein p12 from different field virus isolates, deduced from the nucleotide sequence of the gene, revealed a high degree of conservation. no mutations were found after adaptation to vero cells, and a polypeptide with similar characteristics was present in an ibrs2-adapted virus. the sequence of the 5' flanking region was conserved among the isolates, whereas sequences downstream of the gene were highly variable in ... | 1992 | 1583733 |
| amino acid sequence and structural properties of protein p12, an african swine fever virus attachment protein. | the gene encoding the african swine fever virus protein p12, which is involved in virus attachment to the host cell, has been mapped and sequenced in the genome of the vero-adapted virus strain ba71v. the determination of the n-terminal amino acid sequence and the hybridization of oligonucleotide probes derived from this sequence to cloned restriction fragments allowed the mapping of the gene in fragment ecori-o, located in the central region of the viral genome. the dna sequence of an ecori-xba ... | 1992 | 1583732 |
| a second envelope glycoprotein mediates neutralization of a pestivirus, hog cholera virus. | several monoclonal antibodies (mabs) raised against hog cholera virus (hcv) reacted with the hcv structural glycoprotein gp44/48 and neutralized the virus. the presence of hcv gp44/48 on the viral surface was directly demonstrated by immunogold electron microscopy. eight anti-hcv gp44/48 mabs were tested by immunoperoxidase assay against a panel of pestivirus strains. each mab showed a distinct pattern of reactivity with hcv strains. it is suggested that the mabs are well suited for epidemiologi ... | 1992 | 1583727 |
| [is feeding of green silage in areas with hog cholera in wild boar a danger for domestic swine herds? experimental study]. | in an experimental study we tested the survival of hog cholera virus (hcv) contained in pieces of muscular tissue and organs from experimentally infected swine after incubation in silage. in big (diameter greater than 20 cm) muscular pieces hcv survived even in excellent mineral acid silage (ph 3.8-4.0) after a storage of 5 months. on the other hand in smaller parts (musculature tissue, organs less than 20 cm diameter) we never found virulent hcv after 3 months of incubation. independent of the ... | 1992 | 1575668 |
| genetic manipulation of african swine fever virus: construction of recombinant viruses expressing the beta-galactosidase gene. | homologous recombination is shown to be specifically induced in vero cells by infection with african swine fever (asf) virus. the frequency of recombination induced by asf virus infection between cotransfecting plasmids is comparable to that found after infection with the prototype poxvirus, vaccinia virus. the induction of recombination is accompanied by replication of the plasmid templates in the asf virus-infected cells. an asf virus insertion/expression plasmid vector containing the escheric ... | 1992 | 1566585 |
| [light and electron microscopic findings in the intestine of spontaneous and experimentally-produced african swine fever]. | light and electron microscopical studies were carried out after experimental induced and spontaneous infection with african swine fever virus. the experimental infection was performed in 18 pigs divided into two groups consisting of 9 animals. the pigs of group i were inoculated with virulent isolate e 70, those of group ii with attenuated isolate e 75. two infected pigs of group i and one control animal were killed on days 3, 5 or 7 p.i., two pigs of group ii and one control animal were killed ... | 1992 | 1559460 |
| african swine fever virus: generation of subpopulations with altered immunogenicity and virulence following passage in cell cultures. | virus subpopulations with variable virulence, immunogenicity, and infectivity to pigs were readily generated by passaging tengani isolate of african swine fever virus, either biologically cloned or uncloned, in vero cell cultures. avirulent virus populations which account for more than 99% of virus in an uncloned preparation of the 27th passage are laboratory artefacts, perhaps do not exist in nature. furthermore, attenuation of virulence did not occur uniformly in all subpopulations newly gener ... | 1992 | 1558888 |
| modulation of splenic macrophages, and swine leukocyte antigen (sla) and viral antigen expression following african swine fever virus (asfv) inoculation. | expression of viral and major histocompatibility complex (mhc) antigens and localization of t cells and macrophages was studied in frozen tissue sections of spleens taken from normal pigs or from pigs inoculated with highly virulent lisbon 60 (l60), or with moderately virulent dominican republic 1978 (dr-ii), african swine fever virus (asfv) isolates. splenic sections from l60 inoculated pigs exhibited a large decrease in macrophage staining, whereas dr-ii infected animals appeared more intensel ... | 1992 | 1550493 |
| expression in vivo and in vitro of the major structural protein (vp73) of african swine fever virus. | the vp73 protein was produced by in vitro transcription and translation from the xho i-bam hi fragment located between the cla i-n and cla i-h fragments of the viral genome. this dna fragment encodes a late mrna of about 2.6 kb detected in infected ms monkey and bhk hamster cells. the transcript was initiated at a site within two bases upstream of the translation initiation codon. the in vitro synthesized polypeptide shows the same molecular weight as the in vivo synthesized polypeptide, suggest ... | 1992 | 1550491 |
| a tissue culture vaccine with lapinized chinese (lc) strain of hog cholera virus (hcv). | the lapinized chinese (lc) strain of hog cholera virus (hcv), was adapted to grow in a cell line from minipig kidney (mpk) where it reached a titer, as determined by immunofluorescence, significantly higher than in rabbits. inasmuch as the immune serum to hcv neutralized the culture-adapted virus, it was concluded that its antigenicity did not undergo any change after adaptation to mpk cells. the mpk-lc adapted virus (mpk-lc-hcv) showed also a higher immunogenic activity in rabbits, in compariso ... | 1992 | 1516363 |
| a review on classical swine fever infections in pigs: epizootiology, clinical disease and pathology. | a review is given on classical swine fever (csf) including epizootiology, clinical disease and pathology. under the item of epizootiology the history of csf is briefly summarized. ways of transmission are described with special reference to csf in wild boars. the chapter about clinical disease includes the description of different courses of csf such as peracute, acute, subacute form and chronic disease with reference to the course of transplacental infection and fate of the progeny associated w ... | 1992 | 1516362 |
| a sensitive dot immunobinding assay for serodiagnosis of african swine fever virus with application in field conditions. | the present work describes a simple dot immunobinding assay (dia) for african swine fever virus (asfv) antibody detection that can be used under field conditions. the assay uses nitrocellulose strips dotted with a cytoplasmic soluble antigen (cs-p) of asfv. the nitrocellulose strips are adhered to a plastic handle. the test serum samples react with the cs-p, and antibodies are detected using a protein a-peroxidase conjugate. both incubations are carried out at 20 c. the efficacy of the dia as a ... | 1992 | 1515486 |
| detection of african swine fever viral antigens in paraffin-embedded tissues by use of immunohistologic methods and polyclonal antibodies. | tissues obtained from pigs inoculated with african swine fever virus (asfv), fixed by vascular perfusion using glutaraldehyde, and embedded in paraffin or araldite were used for an immunohistologic electron microscopic study. to detect asfv antigens, 4 methods were used on paraffin sections with or without pretreatment of the tissues. use of biotinylated anti-asfv antiserum combined with avidin-biotin complex and peroxidase proved to be the most suitable method, and antigen was detected in tissu ... | 1992 | 1510327 |
| experimental transmission of african swine fever virus by the soft tick ornithodoros (pavlovskyella) marocanus (acari: ixodoidea: argasidae). | a total of 1,600 ornithodoros (pavlovskyella) marcocanus larvae were fed on a pig with a viremia of 10(7.4) had50/ml of african swine fever virus (asfv). infected larvae were sampled daily for 15 d, and nymphs were sampled at least once per instar until they became adults. initial titers of 10(4.48) had50 per larva declined to 10(4.04) within 2 d. larval titers reached a maximum of 10(6.0) had50 per larva 10 d after the infective blood meal. nymphs of each instar were fed on a susceptible pig an ... | 1992 | 1495075 |
| analyses of the primary in vitro responsiveness of non-immune porcine peripheral blood mononuclear cells with reference to immunization by african swine fever virus antigen and treatment with leucine methyl ester. | peripheral blood mononuclear cells (pbmc) from non-immune pigs were immunized in vitro using african swine fever (asf) virus antigen with concomitant mitogenic stimulations known to have varying effects on b and t lymphocyte activity. none of these conditions, including those previously reported as being successful for the in vitro immunization of non-immune porcine pbmc with asf virus antigen, supported the induction of specific antibody. due to the reports on in vitro immunization of human pbm ... | 1992 | 1487303 |
| approaches to the identification of non-essential genes of african swine fever virus. | it is poorly understood why vaccines could not be developed for the control and prevention of african swine fever (asf) virus infection. the aim of our study was to identify genes non-essential for asf virus replication because there were indications that certain viral gene products, which apparently are non-essential for viral replication, conferred protection from death due to asf. a cosmid library representing the genome of asf virus strain france 64 was established and characterized. then, i ... | 1992 | 1481351 |
| survival of hog cholera virus (hcv) in sausage meat products (italian salami). | survival of hog cholera virus (hcv) was determined in several sausage meat products (italian salami) prepared with meats from experimentally infected hogs slaughtered at the peak of disease. meats were processed following the technology applied by the main factories of the typical italian production. the survival of hcv was assessed through inoculation in both pk 15 cell monolayers and fully susceptible piglets. in all types of sausages examined hcv was detected up to 75 days of curing by piglet ... | 1992 | 1476864 |
| african swine fever virus infection in the soft tick, ornithodoros (alectorobius) puertoricensis (acari: argasidae). | in total, 1,186 second instar ornithodoros (alectorobius) puertoricensis fox second instars were fed on a pig when it had a viremia of 10(5.2) hemadsorption units (had50/ml) and 420 second-instar o. puertoricensis were fed on an uninfected pig. subsequent blood meals for ticks in both groups were from uninfected pigs. the effects of african swine fever virus (asfv) infection on o. puertoricensis populations were evaluated for the following parameters: mortality; mean time to death; percentage mo ... | 1992 | 1460641 |
| evaluation of the complex trapping blocking-elisa in a serological survey during the belgian classical swine fever epizootic in 1990. | 1992 | 1455586 | |
| a comparison of the pathogenicity of two strains of hog cholera virus. 2. virological studies. | quantitative and qualitative differences were demonstrated in the amount of virus in a range of tissues from pigs infected with either the weybridge or new south wales (nsw) strains of hog cholera (hc) virus. the titre of the weybridge strain in samples, as assessed by either virus titration in cell culture or by the density of specific fluorescing cells in tissue sections, was higher than that for the nsw strain. this correlated with the greater severity of the clinico-pathological syndrome ind ... | 1992 | 1445071 |
| a comparison of the pathogenicity of two strains of hog cholera virus. 1. clinical and pathological studies. | the virulence of a strain of hog cholera virus isolated during an outbreak of mild disease in pigs in new south wales in 1960/61 (the nsw strain) was compared over 11 days with that of a virulent strain by inoculating 8 pigs with each virus and comparing the ensuing clinical signs and pathology. both viruses caused persistent pyrexia and leukopenia, the nsw strain 4 to 5 days and the virulent strain 3 days, after inoculation. few other clinical signs were observed in the pigs inoculated with the ... | 1992 | 1445070 |
| [immune reactions to the african swine fever virus]. | host immune reactions to african swine fever virus variants differing in their virulence were studied comparatively. their obvious variabilities in antibody induction to some polypeptides active in antibody-dependent cell cytotoxicity and cytotoxic t-lymphocytes were demonstrated. t-helpers of immune pig splenocytes were found to recognize the cells infected with avirulent but not virulent virus variants. the described differences were not connected with the changes in sla-1 antigen expression i ... | 1992 | 1441445 |
| [polypeptides p14 and p31 of the african swine fever virus--early proteins located on the membrane of the infected cell]. | african swine fever virus polypeptides p14 and p31 are synthesized in the presence of phosphonacetic acid which inhibits viral dna replication, and therefore they are early viral proteins. these polypeptides were found to be localized on plasma membranes by immunofluorescence with monospecific antisera and monoclonal antibodies and by selective solubilization of infected cells. the p14-specific antibodies mediate complement-dependent cytolysis and antibody-dependent cytotoxicity of the cells inf ... | 1992 | 1441444 |
| molecular characterisation of a dna ligase gene of the extremely thermophilic archaeon desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases. | a 3382 bp fragment containing a gene for a dna ligase from the extremely thermophilic, acidophilic, and facultatively anaerobic archaeon (archaebacterium) desulfurolobus ambivalens was cloned and sequenced. the deduced amino acid sequence (600 amino acids, 67619 molecular weight) showed 30-34% sequence identity with the atp-dependent eucaryal (eukaryotic) dna ligases of schizosaccharomyces pombe, saccharomyces cerevisiae, the human dna ligase i, and with the vaccinia dna ligase. distant similari ... | 1992 | 1437556 |
| localization of african swine fever viral antigen, swine igm, igg and c1q in lung and liver tissues of experimentally infected pigs. | an immunohistological study was carried out on lungs and livers of pigs experimentally infected with two different african swine fever virus (asfv) isolates. asfv antigen, swine immunoglobulins (igm and igg) and (clq) complement were demonstrated in both organs at different stages of infection. the asfv antigen was mainly found in mononuclear phagocytic system (mps) cells. immunoglobulins and complement were observed in plasma, infected and non-infected phagocytic cells and cell debris. these fi ... | 1992 | 1430349 |
| distribution of asfv antigens in pig tissues experimentally infected with two different spanish virus isolates. | lymph nodes, spleen, liver, lung and kidney obtained from pigs experimentally infected with two african swine fever virus (asfv) isolates of differing virulence were fixed by perfusion with glutaraldehyde and embedded in paraffin. an immunoperoxidase technique using a polyclonal anti-asfv serum was performed on tissue sections in order to detect asfv antigen. the distribution of asfv antigen in such infected organs is shown and the differences between both infections compared and discussed. mono ... | 1992 | 1414093 |
| african swine fever virus-induced dna polymerase is resistant to aphidicolin. | african swine fever virus (asfv) induces the synthesis of a virus-specific dna polymerase, which is inhibited by phosphonoacetic acid and cytosine arabinoside. in contrast to all other alpha-like dna polymerases of dna viruses, asfv-specific dna polymerase is resistant to aphidicolin. concentrations of the drug as high as 160 microm had no effect on virus production or plaquing efficiency. the resistance of asfv dna polymerase to aphidicolin was confirmed by analyzing the effect of the drug on v ... | 1992 | 1413523 |
| transcriptional analysis of multigene family 110 of african swine fever virus. | a transcriptional analysis of the 3.2-kb region of the african swine fever virus genome containing the five members of the multigene family 110 is presented. the mrnas corresponding to the genes studied have short leader sequences with no intervening aug codons before the translational start site, and their 3' ends map within a conserved sequence motif formed by a stretch of seven or more consecutive thymidylate residues. the possible role of this sequence as a signal for the 3'-end formation of ... | 1992 | 1404609 |
| african swine fever virus infection in the iberian soft tick, ornithodoros (pavlovskyella) marocanus (acari: argasidae). | one thousand six hundred ornithodoros (pavlovskyella) marocanus velu larvae were fed on a pig infected with african swine fever virus (titer: 10(7.4) had50/ml), and 1,600 larvae were fed on an uninfected pig. ticks in each group were compared for mortality rates, mean time to death for ticks that died, mean time from feeding to either molting or eclosion, percentage of ticks that eclosed or molted, and the number of blood meals per nymph or instar. cumulative virus-induced mortality for all imma ... | 1992 | 1404269 |
| characterization and pathogenicity for pigs of a hog cholera virus strain isolated from wild boars. | one hog cholera virus strain isolated from an outbreak of the disease in a wild boar breeding herd in brittany (france) in 1990 has been characterized with a panel of monoclonal antibodies to hog cholera virus and ruminant pestiviruses: the strain was found to be indistinguishable from that of other domestic pig isolates. the pathogenicity of the strain to domestic pigs was evaluated by infecting intranasally, intramuscularly and by contact 17 specific pathogen-free 6-week- and 12-week-old pigs. ... | 1992 | 1387299 |
| flow cytometric analysis of african swine fever virus-induced plasma membrane proteins and their humoral immune response in infected pigs. | african swine fever (asf) virus-induced plasma membrane proteins may contribute to the protective immune response against the disease since they can be involved in the antibody-mediated lysis of infected cells. in this study we describe the regulation of asf virus-induced plasma membrane protein expression and its antibody induction in pigs after viral infection by flow cytometric analysis. more than 80% of infected cells contained viral antigens on the surface membranes at 6 hr postinfection (h ... | 1992 | 1376539 |
| analysis of t lymphocyte subsets proliferating in response to infective and uv-inactivated african swine fever viruses. | the proliferative response to infective and uv-inactivated african swine fever virus was analyzed in cells from pigs surviving an experimental infection with attenuated virus. all the pigs showed strong dose-dependent proliferative responses to both infective and uv-inactivated virus. this response was also observed when nitrocellulose-bound solubilized virus proteins were used in the assay. heterologous isolates also induced proliferation, however it was significantly lower than that induced by ... | 1992 | 1362304 |
| thermal and ph stability of pestiviruses. | three strains/isolates of hog cholera virus (hcv) and two strains/isolates each of cytopathogenic (cp) and non-cytopathogenic (ncp) biotype of bovine virus diarrhoea virus (bvdv) were each exposed to ph 3, 3.5 and 4 at 4 degrees c, 21 degrees c and 37 degrees c in a number of combinations. infectivity titration and half-life determinations following correlation and regression analysis showed a significant temperature-dependent shortening of half-lives within the ph range investigated. at ph 3, m ... | 1992 | 1335310 |
| african swine fever virus encodes a gene with extensive homology to type ii dna topoisomerases. | nucleotide sequencing of a virulent african swine fever virus (asfv) isolate (malawi lil20/1) identified an open reading frame of 1191 amino acid residues encoding a protein of 134.9 kda. this gene mapped to the sali i and j restriction endonuclease fragments of the asfv genome. the predicted polypeptide was found to share 21.1% identity over a 1077 amino acid region with the human type ii dna topoisomerase. the sequence is compared to other type ii dna topoisomerases and the possible roles in a ... | 1992 | 1335084 |
| hog cholera diagnostic techniques. | clinical signs and lesions can sometimes provide the basis for a presumptive diagnosis of hog cholera (hc). however, an accurate diagnosis requires laboratory testing. the usual procedure for the detection of viral antigen is the examination of cryostat sections stained with fluorescein-conjugated hc antiserum. a more definitive technique is isolation of the virus in pk-15 cell cultures and identification of the viral antigen in cells using an hc fluorescent antibody conjugate. as bovine viral d ... | 1992 | 1325335 |
| the hog cholera virus. | hog cholera virus (hcv) is a spherical enveloped particle of about 40-60 nm dia. the viral genome is a single strand rna of about 12,000 bases with positive polarity. one single large open reading frame codes for presumably four structural, i.e. three glycoproteins and a core protein, and about three to five nonstructural proteins. the functional role is not yet fully clear for all viral proteins. hcv belongs to the pestivirus group and it is closely related to bovine viral diarrhoea and border ... | 1992 | 1325334 |
| protection of piglets born from ruminant pestivirus experimentally infected sows, and their contacts, to the challenge with hog cholera virus. | two groups, a and b, of two specific pathogen-free pregnant sows were experimentally infected between the 25th and 29th days post-breeding with two strains of ruminant pestivirus: nadl cytopathic bovine viral diarrhoea virus for group a and aveyron non-cytopathic border disease virus french strain for group b. two other pregnant sows (group c) were kept uninoculated as control. when 7 weeks old, 8 piglets of group c were put in contact with 4 piglets of group a (group d), and 8 other piglets of ... | 1992 | 1324630 |
| identification of an african swine fever virus gene with similarity to a myeloid differentiation primary response gene and a neurovirulence-associated gene of herpes simplex virus. | here we describe an open reading frame (lmw23-nl) in the african swine fever virus genome that possesses striking similarity to a murine myeloid differentiation primary response gene (myd116) and the neurovirulence-associated gene (icp34.5) of herpes simplex virus. in all three proteins, a centrally located acidic region precedes a highly conserved, hydrophilic 56-amino-acid domain located at the carboxy terminus. lmw23-nl predicts a highly basic protein of 184 amino acids with an estimated mole ... | 1992 | 1323711 |
| evolution of thymidine and thymidylate kinases: the possibility of independent capture of tk genes by different groups of viruses. | phylogenetic analysis of viral and cellular thymidine and thymidylate kinases was performed using computer-assisted methods. multiple alignments and tentative phylogenetic trees were generated for the two families of these enzymes, which include a) thymidine kinases (tk) of mammals, poxviruses, african swine fever virus, e. coli, and bacteriophage t4; and b) thymidylate kinases (thyk) of yeast and poxviruses and distantly related herpesvirus proteins with both enzymatic activities. analysis of t ... | 1992 | 1317076 |
| a gene homologous to topoisomerase ii in african swine fever virus. | a putative topoisomerase ii gene of african swine fever virus was mapped using a degenerate oligonucleotide probe derived from a region highly conserved in type ii topoisomerases. the gene is located within ecori fragments p and h of the african swine fever virus genome. sequencing of this region has revealed a long open reading frame, designated p1192r, encoding a protein of 1192 amino acids, with a predicted molecular weight of 135,543. open reading frame p1192r is transcribed late after infec ... | 1992 | 1316688 |
| the pestiviruses. | 1992 | 1315479 | |
| a ubiquitin conjugating enzyme encoded by african swine fever virus. | the post-translational modification of proteins by covalent attachment of ubiquitin occurs in all eukaryotes by a multi-step process. a family of e2 or ubiquitin conjugating (ubc) enzymes catalyse one step of this process and these have been implicated in several diverse regulatory functions. we report here the sequence of a gene encoded by african swine fever virus (asfv) which has high homology with ubc enzymes. this asfv encoded enzyme has ubc activity when expressed in escherichia coli since ... | 1992 | 1310934 |
| heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses. | in order to analyse the expression of the non-structural (ns) protein p80/p125 in cells infected with different pestiviruses at the protein level, radioimmunoprecipitations with the pestivirus-specific monoclonal antibody (mab) bvd/c16 were performed. cell lysates infected with cytopathic (cp) and non-cytopathic (ncp) bovine viral diarrhoea (bvd) virus strains and isolates, and with hog cholera (hc) virus strains were analysed. from cpbvd virus-infected cells, the mab precipitated one or more pr ... | 1992 | 1309861 |
| genes homologous to ubiquitin-conjugating proteins and eukaryotic transcription factor sii in african swine fever virus. | the nucleotide sequence of the 6004-bp ecori i fragment of african swine fever virus dna has been determined. translation of the sequence revealed eight closely spaced open reading frames (orfs), three of them reading rightward and five leftward. northern blot hybridization analysis indicated that orfs i73r and i78r were transcribed early in infection, whereas orfs i177l, i196l, and i329l were expressed at late times. transcripts for orfs i215l, i226r, and i243l were detected at low levels in ea ... | 1992 | 1309282 |
| a preliminary map of epitopes on envelope glycoprotein e1 of hcv strain brescia. | monoclonal antibodies (mabs) directed against envelope glycoprotein e1 (gp51-54) of hog cholera virus (hcv) strain brescia have been shown to recognize four different antigenic domains a, b, c and d. epitopes of within domain a have mainly been found conserved among hcv strains, whereas epitopes within domains b, c and d are not conserved. we used transiently expressed hybrid e1 genes of hcv strains brescia and "c" to map the non-conserved epitopes on e1. epitopes in domains b and c are located ... | 1992 | 1282755 |
| muscidae (diptera): experimental vectors of hog cholera virus. | 1976 | 1263216 | |
| [experiments to determine the immunizing dosages in a single vaccinal dose of dried, lapinized k vaccine against swine plague]. | investigated was the amount of the immunizing doses per one vaccinal pig dose of five operative numbers of the lapinized k vaccine against swine fever. for this purpose pigs were immunized with 100, 200, 300, 400, and 500 fold dilutions of the vaccinal dose. a couple of weeks later the pigs were infected with 10(6)ld50 each of a pathogenic swine fever virus. it was found that the immunizing doses for the individual operative numbers of the vaccine vary in number from 100 to 400 in one vaccinal d ... | 1976 | 1258353 |
| [immunological reactivity of suckling pigs born to sows immunized against the lapinized k vaccine]. | the lapinized k vaccine was used to immunize 25 five-day-old pigs and 27 ten-day-old pigs. a group of 10 pigs were also included in the experiment as controls. all animals originated from eight sows treated with a lapinized k vaccine on the 70th day of pregnancy. on the 14th day following vaccination part of the pigs of the two test groups were revaccinated. three months later half of the immunized pigs were infected with a pathogenic swine fever virus. the remaining animals were infected then i ... | 1975 | 1241625 |
| [antibody formation in swine vaccinated with a lapinized k-vaccine and serum against hog cholera]. | immunized were experimentally a total of 10 pigs with 2 cu. cm lapinized k vaccine and 10 cu. cm swine fever serum each. reimmunization was performed a month later using only vaccine. after three and a half months the experimental pigs were included into the group of pigs producing serum against swine fever that were preliminary simultaneously immunized against hog cholera by the classical method. swine fever antibodies in the serum of the experimental pigs showed a concentration that was equal ... | 1975 | 1239855 |
| [viability of the strein k hog cholera virus in the organism of swine]. | 1975 | 1239847 | |
| ultrastructural study of african swine fever virus replication in cultures of swine bone marrow cells. | cultures from pig bone marrow cells were infected with asfv and the replication cycle was studied. in the cytoplasmic replication areas there are a differentiation of membrane segments. some of them are polygonal, which give rise to virus particles. an over production of viral coded materials, including non-polygonal membranes seems to be an important feature of the replicative cycle of asfv in this cell system. viruses are released enveloped with a cellular membrane. paracrystalline arrays of v ... | 1975 | 1239040 |
| stabilization of hog cholera virus by dimethyl sulfoxide. | the stability of hog cholera virus through five freeze-thaw cycles in the presence and absence of dimethyl sulfoxide was studied. in the absence of dimethyl sulfoxide the hog cholera virus titer was reduced 52% to 91% following successive freezing and thawing cycles. however, when dimethyl sulfoxide was added to the viral suspension the virus titer appeared to remain the same after the same number of freezing and thawing cycles. | 1975 | 1236769 |
| occurrence of contaminating viruses in various swine fever virus strains. ii. studies on gillespie's cytopathic type-a strain and on other strains. | 1975 | 1233873 | |
| the basis of arbovirus classification. | the biologically defined set of arboviruses contains well over 300 separate viruses which have been subdivided into some 40 serological groups on the basis of antigenic cross-reactivity. more than three quarters of all arboviruses can now bw placed into one of the following five major taxonomic genera based upon the fundamental properties of the virion: alphavirus, flavivirus, orbivirus, rhadovirus, bunyavirus. there are 20 alphaviruses, representing serological group a, and 57 flaviviruses in s ... | 1975 | 1207193 |
| the use of a microtitration technique for the routine assay of african swine fever virus. brief report. | 1975 | 1168046 | |
| [serological identification and behavior of the highly virulent swine fever virus in immunoelectrophoresis]. | 1975 | 1136613 | |
| [comparison of 2 in vitro methods of titration of antibodies neutralizing the classic swine plague virus]. | swine fever virus neutralization have been studied by the following method: variable plaque forming units numbers are mixed with a predetermined serum dilution. infectivity is measured before (vo) and after (vr) neutralization reaction. neutralization index (n.i. = logvo/v) represent the difference between the two titers. it had been demonstrated that mass law is a good approximation to describe swine fever virus neutralization. so the most useful form in which to express the relation between n. ... | 1976 | 1036215 |
| plaque formation by african swine fever virus in chick embryo fibroblasts in the absence of co2 atmosphere. | a plaque assay method using asfv previously adapted to growth in chick embryo fibroblasts is described. chick embryo fibroblast monolayers under bactoagar or methylcellulose have been employed using cysteine, arginine, deae-dextran and hepes as additives. plaque production was optimal under methylcellulose. hepes rendered the plaques more clear when used with the overlay. arginine enhances plaque formation with bactoagar, and deae-dextran doubles the plaque size. the growth curve of asfv in chic ... | 1976 | 1033755 |
| [studies of the carrier state and excretion of the pathogenic plague virus in vaccinated swine]. | investigations were carried out on the presence of carriers and the excretion of the virulent swine fever virus in pigs vaccinated with the lapinized vaccine strain k. on the 15th, 25th, and 35th day following injection with the virulent virus a pig was killed each time and material was taken for morphologic studies and immunofluorescence microscopy. the results of the investigations were negative except for two pigs the cerebrum of which showed perivascular, slightly to moderately expressed lym ... | 1976 | 1030872 |
| swine fever: influence of passive immunity on pig immune response following vaccination with a live virus vaccine (thiverval strain). | the influence of passive immunity on the immune response to swine fever virus (s.f.v.) was investigated in pigs injected with variable amounts of s.f.v. antibodies instead of piglets from immune sows. the passive immunity suppresses the primary serum antibody response normally observed after vaccination with the thiverval strain of s.f.v. this inhibition is either partial or complete depending on the amount of injected antibodies. whatsoever the passive immunity intensity, a priming occurred (ev ... | 1976 | 1028380 |
| [the propagation of swine fever virus in cultures of macrophages and lymphocytes of normal and immune pigs (author's transl)]. | swine fever virus is replicated in the cells of the lymphoid complex. during the course of the disease cell-destruction, leucopenia and a disturbance of globulin and transferrin production is described. in the case of recovering firstly the leucocyte population, specially the lymphocytes, later on antibody titer increase. the production of virus neutralizing antibodies is not recognizable to be the cause of a recovery, the latter seems to be initiated by the production of a newly formed cell pop ... | 1976 | 1015008 |
| isolation and segregation of non-haemadsorbing strains of african swine fever virus. | 1976 | 1014300 | |
| sub-clinical swine fever: a survey of neutralizing antibodies in ther sera of pigs from herds having reproductive failures. | sera harvested from breeding farms where reproductive failures were observed but where swine fever vaccination was not carried out, where tested for the presence of neutralizing antibodies specific for swine fever virus. neutralization tests were performed in tissue culture using two viruses strains: the american serological variant "331" strain isolated by mengeling (1969) and the virulent "normal strain" alfort. for comparison, sera harvested from healthy, vaccinated and unvaccinated breeding ... | 1976 | 984714 |
| sensitivity of swine buffy coat culture to infection with hog cholera virus. | the susceptibility of swine buffy coat (bc) cultures inoculated with hog cholera (hc) virus in the presence of homologous antiserum was greater than that of a pig kidney (pk-15) cell line similarly inoculated. the virus was isolated from bc cultures grown in the presence of 0.1% hyperimmune serum, whereas it could not be consistently recovered from the pk-15 cell line in which hyperimmune serum concentrations exceeded 0.025%. maximal viral titers in bc culture were reached between postinoculatio ... | 1976 | 984563 |
| purification and physicochemical characteristics of african swine fever virus. | methods for the purification of african swine fever virus (asfv) and its dissection into two fractions are described. the difficulties which have been encountered previously in attempts to purify the virus, namely contamination with large amounts of cellular constituents and aggregation of the virus particles, have been overcome by treatment with tween 80 and by the use of 1 m-nacl in the sucrose gradients. five major polypeptides, mol. wt. 10(3) x 125 (vp1), 76 (vp2), 50 (vp3), 44 (vp4) and 39 ... | 1976 | 965949 |
| transmission studies with african swine fever virus. the early distribution of virus in pigs infected by airborne virus. | 1977 | 908774 | |
| transmission studies with african swine fever virus. infections of pigs by airborne virus. | 1977 | 908773 | |
| local immunity in the pig respiratory tract. ii. -- relationship of serum and local antibodies. | antibody passage from blood to respiratory secretions was studied on pigs. the animals were passively immunized and have a high anti-hog cholera serum antibody titer. with normal animals as with swine influenza animals, no serum antibody could be recovered neither in buccopharyngeal secretions nor in lung washings: it seems that in such conditions no detectable transudation occurs from blood to local secretions. following intranasal inoculation of swine influenza virus or intramuscular injection ... | 1977 | 907266 |
| [pathomorphological studies of the infections manifested in swine after vaccination with lapinized strain k vaccine against hog plague]. | pigs that had died after vaccination with a lapinized k strain of the swine fever virus at the persistence of another infection showed morphologic and histopathologic changes that were largely similar to those observed in cases of classic hog cholera. the difference consisted in the frequence and intensity of the lesions. histopathologically essential proved the investigation of the brain. the latter usually presents a prevailing activation and hyperplasia of the capilary endothelium, while in c ... | 1977 | 898647 |
| cellular immunity demonstrated in pigs infected with african swine fever virus. | twenty-two pigs infected with african swine fever virus (asfv) were used to demonstrate delayed hypersensitivity (dh) in vitro by the leukocyte migration-inhibition test. the results indicated that asfv-infected pigs developed dh against asfv antigen (asf antigen) as early as 20 days after inoculation, and the presence of viremia did not interfere with the leukocyte migration-inhibition test. three asfv-infected pigs that were also sensitized to mycobacterium developed dh against both asf antige ... | 1977 | 835865 |
| [a new inactivated vaccine against classical swine fever]. | authors used a wild strain of classical swine fever virus alfort a19, grown on pk15 cell culture. virus is concentrated by ultrafiltration technique, inactivated with glycidaldehyde and then mixed with an oil adjuvant. results have brought into evidence the absolute innocuousness of this vaccine and animals given 2 doses of 2.5 ml or 1 dose of 5 ml were resistant to a challenge 2 months after vaccination. protection lasts one year, at least. | 1976 | 828543 |
| [effect of heating on the survival of swine fever virus in pasteurised canned ham from experimentally infected animals (author's transl)]. | experimentally infected pigs were slaughtered during viraemia. the hams were prepared according to the industrial process and packed in tins with an average content of 5.6 kg. the tins were then heated in the course of which temperatures in the centre of the hams reached maximum values varying between 62.5 and 68.5 degrees c. within 24 hours of heating the centre of the ham was homogenised and one of two piglets were inoculated with 40 to 80 ml of a 25 per cent suspension. virus was recovered fr ... | 1976 | 827818 |
| [attempts to purify strain k of the virus of classical swine plague]. | in the treatment of spleens and lumph nodes of rabbits infected with strain k, with the use of freon 113 the virus was freed from proteins up to 92-96 per cent. the infective power of the purified virus proved equal to that of the initial non-purified virus proved equal to that of the initial non-purified virus, and this points to the full extraction of the virus itself. the antigenic properties of the purified virus were demonstrated through agar gel precipitation with normal and hyperimmune se ... | 1975 | 814700 |
| [pathomorphological changes in swine plague and their diagnostic value]. | 1978 | 751319 | |
| influence of colostral antibodies on pig immunization against hog cholera virus. | 1978 | 747321 | |
| cellular and humoral immune response in pigs given vaccinal and chronic hog cholera viruses. | humoral immune response (seroneutralization) and cellular immune response (lymphocyte-stimulation test) were analyzed in pigs inoculated with low virulent strains of hog cholera virus or vaccinated with a live-virus vaccine. vaccinated animals exhibited an intense neutralizing antibody production, but cellular immune response was not detected. neutralizing antibodies were not found in infected pigs, but a brief cellular immune response (18 days after inoculation) was observed. | 1978 | 736342 |
| diagnostic and prophylactic problems connected with the control of foot and mouth disease, brucellosis, tuberculosis and swine fever in italy. | 1977 | 618072 | |
| [hog cholera virus: influence of colostral passive antibody on immune response of pig following vaccination with the rabbit adapted chinese strain (author's transl)]. | using the rabbit adapted chinese strain of hog cholera, active immunization of piglets having passive colostral antibodies was studied. 65 piglets born from 11 sows were used. concerning sows, vaccination was performed 5-6 months and 1 month before service (3 sows), 30 days (2 sows) and 60 days (3 sows) after service. divided in 5 lots, piglets were vaccinated at 4 different periods after birth (15, 30, 60 and 90 days). hog cholera immunity was determined for each animal by means of kinetic of s ... | 1977 | 606138 |
| comparison of the immune response in serum and bucco-pharyngeal secretions following immunization by different routes with a live hog cholera virus vaccine (thiverval strain). | the influence of vaccination route on local and systemic immune response was studied with hog cholera virus vaccine. kinetics of antibody synthesis in serum and bucco-pharyngeal secretion was observed. results show that, whatever the vaccination route (intranasal or intramuscular) a good systemic and local immune response were induced and pigs resisted to a virulent challenge. however the highest local immune response was obtained by intranasal vaccination with large vaccine dosage (10(7) p.f.u. ... | 1977 | 596794 |
| [clinical and pathomorphological studies of rabbits injected with virulent and lapinized swine plague virus]. | investigations were carried out to establish the clinical and morphological reactions in rabbits following the consecutive infection with the virulent vratsa strain and the lapinized k strain of the swine fever virus. it was found that although the virulent strain did not cause a thermal response there were morphological lesions in the body of the rabbits, characterizing the presence of an immune response. the latter was shown to inhibit the thermal reaction in the animals after infecting with l ... | 1978 | 571168 |
| structural similarities of hog cholera virus with togaviruses. | hog cholera virus grown in pk-15 cells was purified by centrifugation through a sucrose cushion followed by sucrose gradient centrifugation. analysis of virus labeled externally with [3h]sodium borohydride on polyacrylamide gel electrophoresis revealed two glycoproteins, gp55 and gp46. a third structural polypeptide, p36, seems not to be glycosylated. the gp46 was also found in the virus-free supernatant of infected cells. it could be demonstrated by radioimmune precipitation of virus labeled wi ... | 1978 | 567468 |
| the replication of african swine fever virus in pig endothelial cells. | 1978 | 566606 | |
| residual viruses in pork products. | partly cooked canned hams and dried pepperoni and salami sausages were prepared from the carcasses of pigs infected with african swine fever virus and pigs infected with hog cholera virus. virus was not recovered from the partly cooked canned hams; however, virus was recovered in the hams before heating in both instances. both african swine fever virus and hog cholera virus were recovered from the dried salami and pepperoni sausages, but not after the required curing period. | 1978 | 564162 |