Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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prp modulates expression of bone matrix proteins in vivo without long-term effects on bone formation. | this experimental study (domestic pig) examined the bone formation after filling defined defects of the frontal skull with autogenous bone or a deproteinized bovine bone matrix (dbbm) in combination with platelet-rich plasma (prp). six groups, both materials with and without prp in two different concentrations (4.1x and 6.5x referring to untreated whole blood) were evaluated at 2, 4, 12, and 26 weeks by means of immunohistochemical staining for different bone matrix proteins, microradiography, l ... | 2006 | 16257592 |
management of wild ungulate populations in italy: captive-breeding, hybridisation and genetic consequences of translocations. | captive-reproduced stocks of some species of ungulates (artiodactyla), and particularly the red deer (cervus elaphus), fallow deer (dama dama), roe deer (capreolus capreolus) and the wildboar (sus scrofa) are more or less extensively translocated in italy, mainly for local reintroductions or restocking of exploited wild populations. however, captive breeding often involves the reproduction of non-indigenous individuals or the production of artificial hybrids. consequently, translocations of capt ... | 2005 | 16244928 |
bone regeneration in osseous defects using a resorbable nanoparticular hydroxyapatite. | this animal study examined the de novo bone formation in bony defects following the insertion of autogenous bone alone versus an injectable nanoparticle hydroxyapatite alone and in combination with 25% autogenous bone. the regenerative potentials of the tested materials were compared with each other. | 2005 | 16243180 |
an epidural cooling catheter protects the spinal cord against ischemic injury in pigs. | using swine, we investigated whether epidural placement of a cooling catheter rather than infusing iced saline solution could protect the spinal cord from ischemia during aortic surgery. | 2005 | 16242463 |
effects of spontaneous breathing during airway pressure release ventilation on respiratory work and muscle blood flow in experimental lung injury. | to evaluate the effects of spontaneous breathing at ambient airway pressure (paw) and during airway pressure release ventilation (aprv) on respiratory work and respiratory muscle blood flow (rmbf) in experimental lung injury. | 2005 | 16236977 |
plasma disappearance of indocyanine green: a marker for excretory liver function? | to investigate whether the plasma disappearance rate of indocyanine green (icg) assessed using a commercially available bedside monitor provides an accurate estimation of cumulative biliary icg excretion in a clinically relevant model of long-term, hyperdynamic porcine endotoxemia. | 2005 | 16231068 |
a novel spleen-preserving laparoscopic technique using radiofrequency ablation in a porcine model. | partial splenectomy is considered to be the optimal management for a variety of diseases. at the same time, laparoscopic procedures are increasingly used because they present certain advantages against their open counterparts. in this study, the safety and efficacy of radiofrequency ablation (rfa) were evaluated in laparoscopic partial splenectomy performed in a pig model. | 2005 | 16228858 |
[proposal for changing the spot checking examination regarding the control of the maintenance of freedom from aujeszky's disease (ad)]. | in germany the last outbreak of aujeszky's disease (ad) in a holding of domestic pigs had been officially confirmed in the year 2000, and, since february 2003 germany is officially confirmed free of ad. since this time the ad free status is monitored according to chapter ii of the annex of the regulation for the protection against aujeszky's disease. as an alternative monitoring protocol two variants of a two step random sampling scheme is proposed which reduces the sample size from a complete i ... | 2005 | 16218180 |
distribution of porcine endogenous retroviruses (pervs) dna in organs of a domestic pig. | domestic pig may serve as the most appropriate organ source for human xenotransplantation in the future. however, there is a serious threat of xenogeneic pathogens transmission, especially porcine endogenous retroviruses (pervs) which are present in genomes of all pigs. the aim of this study was to monitor the prevalence and distribution of perv dna in organs of a domestic pig. | 2005 | 16218033 |
the cd11a partner in sus scrofa lymphocyte function-associated antigen-1 (lfa-1): mrna cloning, structure analysis and comparison with mammalian homologues. | lymphocyte function-associated antigen-1 (lfa-1, cd11a/cd18, alphalbeta2), the most abundant and widely expressed beta2-integrin, is required for many cellular adhesive interactions during the immune response. many studies have shown that lfa-1 is centrally involved in the pathogenesis of several diseases caused by repeats-in-toxin (rtx)-producing bacteria. | 2005 | 16216120 |
gut luminal microdialysis of glycerol as a marker of intestinal ischemic injury and recovery. | to evaluate microdialysis as a method to assess different degrees of intestinal damage and recovery during ischemia and reperfusion; to evaluate information obtained from microdialysis catheters in the peritoneum, the gut wall, and the gut lumen. | 2005 | 16215382 |
superficial contact cryoablation attenuates experimentally created lung air leakage. | previously, we, and others found that cryoablation on normal lung produced localized pulmonary hemorrhage and edema, causing obliteration of air space. therefore, we hypothesized that lung air leakage may be diminished by this procedure. in the present study, we examined if cryoablation can attenuate experimentally created lung air leakage. male domestic pigs (n=4) underwent a thoracotomy. the lung was resected approximately 5 mm in diameter and 1mm in depth to create air leakage lesions. an arg ... | 2005 | 16202403 |
proliferation and apoptosis of spermatogonia in postpuberal boar (sus domesticus) testes with spontaneous unilateral and bilateral abdominal cryptorchidism. | cryptorchidism is a frequent male sexual disorder in mammals, which affects the histology of the tunica propria, interstitial tissue, blood vessels, seminiferous epithelium and testis functioning. in this paper, proliferation and apoptosis were examined in the seminiferous epithelium of both testes from unaffected boars and from boars suffering unilateral and bilateral cryptorchidism. in germ cells, proliferation was studied using the immunohistochemical pcna technique, and apoptosis was analyse ... | 2005 | 16185749 |
novel calretinin and reelin expressing neuronal population includes cajal-retzius-type cells in the neocortex of adult pigs. | cajal-retzius cells and their secreted product reelin are essential for the lamination of the cerebral cortex. in all species studied to date cajal-retzius cells form a transient neuronal population that almost completely disappears from the neocortex postnatally. recently, in the hippocampal formation of adult domestic pig, we have found a large calretinin- and reelin-immunoreactive cell population that morphologically corresponded to cajal-retzius cells. in the present study, we examined calre ... | 2005 | 16181738 |
repair of pig dura in vivo using temperature controlled co(2) laser soldering. | the purpose of this study was to demonstrate that laser soldering might be successfully used for closing holes or cuts in the dura layer, which encapsulates the brain. | 2005 | 16180219 |
pimecrolimus permeates less than tacrolimus through normal, inflamed, or corticosteroid-pretreated skin. | the permeabilities of normal human and normal, inflamed, or corticosteroid (cs) pretreated skin of young domestic pigs for pimecrolimus and tacrolimus were compared in vitro, using franz-type diffusion cells. the test articles were either used as 1.0% solutions or as the marketed formulations (elidel 1% cream, protopic 0.1%, and 0.03% ointment). in normal human skin, the permeation rate of pimecrolimus from the 1% cream was about sixfold lower than that of tacrolimus from 0.1% ointment and by a ... | 2005 | 16176283 |
intra- and inter-genotypic size variation in the central variable region of the 9rl open reading frame of diverse african swine fever viruses. | african swine fever (asf) viruses are characterised by numerous p72 genotypes, but by low levels of intra-genotypic variation, particularly in domestic pig associated genotypes. as it is precisely these viral lineages that are involved in outbreaks of the disease it is imperative that alternative, more informative gene regions be identified which are suitable for intra-genotypic resolution of relationships. to this end, the central variable region (cvr) of the 9rl open reading frame of diverse a ... | 2005 | 16175341 |
contribution to comprehension of image formation in confocal microscopy of cornea with rostock cornea module. | to investigate the influence of refractive index of aqueous humour on imaging of corneal endothelium in confocal microscopy. to clarify the phenomenon of dark endothelial and bright epithelial cell membranes in confocal images of corneas. | 2005 | 16170131 |
cloning and mapping of the porcine cytochrome-p450 2e1 gene and its association with skatole levels in the domestic pig. | the porcine cytochrome-p450 2e1 (cyp2e1) gene was isolated by screening a pig bac library and partially sequenced. this sequence information was used to identify six single nucleotide polymorphisms (snps) within the cyp2e1 gene and its promoter. in addition, a microsatellite marker tightly linked to the cyp2e1 gene was subcloned from the bac. one of these markers was used to map the cyp2e1 gene distal of swc27 on ssc14, well outside reported quantitative trait loci on ssc14 for skatole, indole a ... | 2005 | 16167985 |
the epidemiology of animal trichinellosis in china. | the epidemiology of animal trichinellosis in china based mainly upon original chinese literature published between 1937 and 2004 is reviewed. the seroprevalence of trichinella infection in herbivores was 0.7% (2/300) in cattle and 0.8% (4/500) in sheep. the prevalence of trichinellosis in naturally infected cattle was 1.2% (2/163). trichinella larvae were detected in 1.4% (3/215) of sheep and in 2.1% (1/47) of beef cattle sold at markets. canine trichinellosis was recorded in 13 provinces, auton ... | 2007 | 16162414 |
resident-intruder trait aggression is associated with differences in lysine vasopressin and serotonin receptor 1a (5-ht1a) mrna expression in the brain of pre-pubertal female domestic pigs (sus scrofa). | aggressive behaviour exhibited by domestic pigs following encounters with unfamiliar individuals is a serious welfare and economical problem. aggression resulting in skin lesions is similarly prevalent in prepubertal pigs of either sex. little is known about the neural circuits and neuropeptides that control aggression in the pig. because there is evidence for the involvement of the vasopressin and serotonergic systems in the regulation of aggressive behaviour in male mammals, we sought differen ... | 2005 | 16159381 |
glutathione improves the function of porcine pulmonary grafts stored for twenty-four hours in low-potassium dextran solution. | flush perfusion with low-potassium dextran is the standard strategy in clinical lung preservation. despite improved outcome, endothelial cell injury and surfactant dysfunction remain a significant problem after lung transplantation. the radical scavenger glutathione has been shown to be responsible for the efficacy of celsior solution in lung preservation. we tested the hypothesis that the addition of glutathione to low-potassium dextran might further improve graft function by ameliorating ische ... | 2005 | 16153941 |
nitrate movement in shallow ground water from swine-lagoon-effluent spray fields managed under current application regulations. | rapid increases in the swine (sus scrofa domestica) population in the 1990s and associated potential for nitrate n pollution of surface waters led the state of north carolina to adopt stringent waste management regulations in 1993. our objectives were to characterize (i) nitrate n movement from waste application fields (wafs) in shallow ground water, and (ii) soil, hydrologic, and biological factors influencing the amount of nitrate n in the adjacent stream. a ground water monitoring study was c ... | 2005 | 16151235 |
oxidant injury occurs rapidly after cardiac arrest, cardiopulmonary resuscitation, and reperfusion. | investigations conducted in cellular models show that reperfusion of ischemic tissue is associated with a burst of reactive oxidant species within minutes after reperfusion. oxidant injury may play a role in the poor outcome typical of people resuscitated from cardiac arrest. the objective of the present study was to determine the presence and timing of oxidant injury in an in vivo model of cardiac arrest. | 2005 | 16148478 |
evaluation of intra-abdominal pressures and gas embolism during laparoscopic partial nephrectomy in a porcine model. | gas embolization is a well documented complication of laparoscopic surgery. we evaluated the association of gas embolization with increased intra-abdominal pressure (iap) in the porcine model during laparoscopic partial nephrectomy with real-time transesophageal echocardiography. | 2005 | 16145470 |
laparoscopic liver resection using radio frequency ablation in a porcine model. | the aim of this study was to assess the feasibility, efficacy, and safety of laparoscopic left lateral lobe resection, using radiofrequency ablation (rfa) with internally cooled tip electrodes, in a porcine model. | 2005 | 16132328 |
levosimendan improves postresuscitation myocardial dysfunction after beta-adrenergic blockade. | in earlier studies, we found that a nonselective beta-adrenergic blocking agent, propranolol, facilitated cardiac resuscitation, reduced postresuscitation myocardial ectopy, and improved postresuscitation survival. however, the potential adverse effects and specifically the negative inotropic actions of propranolol prompted our further investigation of the potential value of a non-beta-adrenergic inotropic drug, levosimendan, in conjunction with propranolol, for minimizing postresuscitation myoc ... | 2005 | 16131457 |
nictitating membrane as a potentially useful postmortem diagnostic specimen for classical swine fever. | the gold standard for diagnosis of classical swine fever (csf) is cell culture virus isolation combined with reverse transcriptase-polymerase chain reaction (rt-pcr) and fluorescent antibody test (fat) in cryosections of tonsils, spleen, various lymph nodes, ileum, and kidney. autolytic and heterolytic samples render correct fat evaluation difficult and can even yield false-negative or ambiguously positive results. to extend the spectrum of csf diagnostic specimens, the authors tested whether th ... | 2005 | 16130991 |
effects of perfluorohexan vapor on gas exchange, respiratory mechanics, and lung histology in pigs with lung injury after endotoxin infusion. | inhaled perfluorohexan vapor has been shown to improve gas exchange and pulmonary mechanics in oleic acid- and ventilator-induced lung injury. however, in the clinical setting, lung injury frequently occurs in the context of systemic inflammation and consecutive lung injury, which may be induced experimentally by intravenous administration of endotoxin. the authors studied whether vaporized perfluorohexan is efficacious during endotoxin-induced lung injury in domestic pigs. | 2005 | 16129984 |
implantation and testing of subretinal film electrodes in domestic pigs. | by definition, an electronic subretinal visual prosthesis requires the implantation of stimulation electrodes in the subretinal space of the eye. polyimide film electrodes with flat contacts were implanted subretinally and used for electrical stimulation in acute experiments in anaesthetised domestic pigs. in two pigs, the film electrode was inserted through a sclerostomy into the vitreous cavity and, subsequently, via a retinotomy into the subretinal space around the posterior pole (ab interno ... | 2006 | 16125172 |
in vivo skin absorption and distribution of the nerve agent vx (o-ethyl-s-[2(diisopropylamino)ethyl] methylphosphonothioate) in the domestic white pig. | the purpose of this study was to characterize the skin absorption and distribution of vx (o-ethyl-s-[2 (diisopropylamino)ethyl] methylphosphonothioate) in the domestic pig in order to evaluate the animal as a potential model for assessing pretreatments against toxic anti-cholinesterase compounds. a liquid droplet (equivalent to a 2 x ld50 dose) of radiolabelled vx was applied to the inner ear-skin of each anaesthetized animal. blood and tissue samples (liver, lung, kidney, heart and skin exposur ... | 2005 | 16119248 |
human endogenous retrovirus expression profiles in samples from brains of patients with schizophrenia and bipolar disorders. | the detection and identification of retroviral transcripts in brain samples, cerebrospinal fluid, and plasma of individuals with recent-onset schizophrenia and schizoaffective disorders suggest that activation or upregulation of distinct human endogenous retroviruses (hervs) may play a role in the etiopathogenesis of neuropsychiatric diseases. to test this hypothesis, we performed a comprehensive microarray-based analysis of herv transcriptional activity in human brains. we investigated 50 repre ... | 2005 | 16103141 |
immune cells in a heterotopic lamb-to-pig bronchial xenograft model. | we developed our porcine model to elucidate the cellular rejection mechanisms of xenografts. bronchial segments from a donor lamb were implanted into domestic pigs. the immunosuppressive regimens consisted of no immusuppression, or of daily oral cyclosporine a (csa) 15 mg/kg, or of everolimus, 1.5 mg/kg, or of both. implants were serially harvested during 17 days. epithelial damage and obliteration were graded histologically, followed by a count of cd4+, cd8+, mhc class ii-expressing cells, and ... | 2005 | 16101731 |
dose response curves for microwave ablation in the cardioplegia-arrested porcine heart. | microwave ablation has been used clinically for the surgical treatment of atrial fibrillation, particularly during valve procedures. however, dose- response curves have not been established for this surgical environment. the purpose of this study was to examine dosimetry curves for the flex 4 and flex 10 microwave devices in an acute cardioplegia-arrested porcine model. | 2005 | 16099735 |
the dielectric properties of the cranial skin of five young captive steller sea lions (eumetopias jubatus), and a similar number of young domestic pigs (sus scrofa) and sheep (ovis aries) between 0.1 and 10 ghz. | to aid in the development of a long-range subcutaneous radio frequency identification tag to monitor the fate of sea lion pups, the dielectric properties of the cranial skin of young female otariids, and possible test subjects of similar size and age, or pigs (sus scrofa) and sheep (ovis aries) were obtained over a frequency range of 0.1-10 ghz at the base of their heads where the tag will be implanted. the resulting curves were similar in shape to adult human skin data, but the values were gene ... | 2005 | 16088057 |
beneficial effects of intravenous administration of lipo-prostaglandin e1 on the ischemic gastric tube in pigs. | lipo-prostaglandin e1 (pge1) is a new preparation of pge1 in which it is bound to lipids to slow pge1 release and delay its rate of metabolism. we investigated the change of the blood flow on impaired microcirculation of the ischemic gastric tube in pigs after saline, unmodified pge1, and lipo-pge1 administration. | 2005 | 16087193 |
validation of portal vein flow measurement by color flow doppler sonography in a porcine model of septic shock. | to compare portal vein flow values gained by color flow doppler sonography with simultaneously derived data from an ultrasound transit time flow probe. | 2005 | 16086179 |
transcatheter placement of a low-profile biodegradable pulmonary valve made of small intestinal submucosa: a long-term study in a swine model. | we sought to investigate a placement of a percutaneous low-profile prosthetic valve constructed of small intestinal submucosa in the pulmonary position in a swine model. | 2005 | 16077416 |
spontaneous cure of domestic pigs experimentally infected by trypanosoma brucei gambiense. implications for the control of sleeping sickness. | the existence of a pig reservoir for human african trypanosomosis (hat) due to trypanosoma brucei gambiense complicates the fight against this disease. this study, reports results obtained from pigs, which were inoculated with the blood of a person, suffering from hat in cameroon. the pigs were reared and kept in the shelter from all contact with glossina, and monitored for 188 days. the seroconversion was checked by agglutination assays for trypanosomosis (catt 1.3 and latex/t.b.gambiense). the ... | 2005 | 16076528 |
molecular epidemiology of african swine fever in east africa. | african swine fever (asf) a lethal, viral hemorrhagic disease of domestic pigs, first reported from east africa in 1921, is still widespread in this region. in order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the c-terminal end of the p72 gene were determined for 77 asf viruses of diverse temporal and species origin occurring in eight east african countries. the number of sites completely conserved across all east african sequences characterized in ... | 2005 | 16052280 |
comparative distribution of sulphur, thiols and disulphides in the porcine stratum corneum. | biochemical, histochemical and cytochemical analyses were used to determine the sulphur contents and the thiol and disulphide distribution in the stratum corneum (sc) of the wild boar (wb), a large domestic pig breed (dp) and the goettingen miniature pig (gmp). the sulphur contents (% dw) were different in the three animal types (wb: 1.70-1.38 body, 0.54 ear; dp: 0.84-0.53 body, 0.50 ear; gmp: 2.28-2.51 body, 2.66 ear). the results of the histochemical analysis of sh- and -s-s- groups were clear ... | 2005 | 16048751 |
fluid resuscitation preserves cardiac output but cannot prevent organ damage in a porcine model during 24 h of intraabdominal hypertension. | according to a previous study, a pathologically increased intraabdominal pressure (iap) reduces cardiac output (co) and results in medium- to high-grade organ damage in a porcine model of the abdominal compartment syndrome (acs). the purpose of this study was to evaluate whether fluid resuscitation can preserve organ integrity together with co. we examined 12 domestic pigs with a mean body weight of 48 kg. we used a co2 pneumoperitoneum to increase the iap to 30 mmhg in 6 animals, and the others ... | 2005 | 16044086 |
identification and characterization of neospora caninum cyclophilin that elicits gamma interferon production. | gamma interferon (ifn-gamma) response is essential to the development of a host protective immunity in response to infections by intracellular parasites. neosporosis, an infection caused by the intracellular protozoan parasite neospora caninum, is fatal when there is a complete lack of ifn-gamma in the infected host. however, the mechanism by which ifn-gamma is elicited by the invading parasite is unclear. this study has identified a microbial protein in the n. caninum tachyzoite n. caninum cycl ... | 2005 | 16041025 |
radiofrequency-enhanced vascular gene transduction and expression for intravascular mr imaging-guided therapy: feasibility study in pigs. | to evaluate the feasibility of radiofrequency (rf)-enhanced vascular gene transduction and expression by using a magnetic resonance (mr) imaging-heating guidewire as an intravascular heating vehicle during mr imaging-guided therapy. | 2005 | 16040894 |
evaluation of surgical models for renal collecting system closure during laparoscopic partial nephrectomy. | to evaluate the utility of the porcine and canine models for collecting system closure after partial nephrectomy involving violation of the renal collecting system. advanced surgical technologies and novel techniques for performing laparoscopic partial nephrectomy are frequently evaluated in an animal model. | 2005 | 16040101 |
changes in the eu legislation on trichinella inspection--new challenges in the epidemiology. | the european union (eu) countries are searching for new ways to certify meat free of trichinella; however, with the expansion of the eu, the acceptance of a unilateral method is complicated by the variability of pig and human trichinellosis among eu countries, where significantly higher prevalence rates have been observed in the newly added eastern countries. several attempts have been made to define trichinella-free areas, but certification of trichinella-free pig production farms appears to be ... | 2005 | 16039781 |
a study of the potential of the pig as a model for the vaginal irritancy of benzalkonium chloride in comparison to the nonirritant microbicide phi-443 and the spermicide vanadocene dithiocarbamate. | a porcine model was established to test the mucosal toxicity potential of a thiophene thiourea (phi-443)-based anti-hiv microbicide and a vanadocene-based spermicide, vanadocene dithiocarbamate (vddtc) in comparison to benzalkonium chloride (bzk). nine domestic pigs (duroc) in nonestrus stage received a single intravaginal application of 2% bzk, 2% phi-443, or 0.1% vddtc-containing gel. at various times after gel application, cell differentials and levels of inflammatory cytokines (il-1beta, il- ... | 2005 | 16036864 |
comparative genetic characterization of porcine circovirus type 2 samples from german wild boar populations. | pcv-2 is involved in "postweaning multisystemic wasting syndrome" (pmws), "porcine dermatitis and nephropathy syndrome" (pdns), respiratory and reproductive disorders, and thereby plays a crucial role in today's swine production world-wide. the virus is apparently ubiquitous in domestic pigs and has also been demonstrated in wild pigs. up to now, a characterization of pcv-2 samples from wild pigs, which might help to estimate the possible role of wild pigs as sources of domestic pig infection, h ... | 2005 | 16023798 |
vaginal contraceptive activity of a chelated vanadocene. | bis(cyclopentadienyl) complexes of vanadium (iv) or vanadocenes are rapid and potent inhibitors of human sperm motility with potential as a new class of contraceptive agents. this study sought to determine the vaginal contraceptive activity of vanadocene dithiocarbamate (vddtc), a stable vanadocene (iv)-chelated complex, using the standard rabbit model as well as the domestic pig as a physiologically relevant animal model for contraception. in experiment i, ovulating new zealand white does in su ... | 2005 | 16022855 |
domesticated pigs in eastern indonesia. | 2005 | 16020714 | |
divergence time of porcine reproductive and respiratory syndrome virus subtypes. | porcine reproductive and respiratory syndrome virus (prrsv) recently emerged in domestic pigs of western europe and north america. although time of emergence was identical on the two continents, genetic composition was markedly different with a clear geographical subtype structure, indicating that subtypes diverged in separate reservoirs prior to emergence. genetic analyses have shown that the most recent common ancestor (mrca) of western european isolates existed around 1980 and that these orig ... | 2005 | 16000650 |
comparative study of tacrolimus and paclitaxel stent coating in the porcine coronary model. | tacrolimus is a potent antiproliferative and immunosuppressive agent allowing for improved endothelial regeneration. the aim of our study was the preclinical evaluation of tacrolimus in a drug eluting nonerodable polymer stent system and its comparison with paclitaxel. | 2005 | 15997345 |
genetic profile and serosurvey for virus infections of japanese wild boars in shikoku island. | to estimate the genetic background of japanese wild boars inhibiting the 4 prefectures of shikoku island (kagawa, tokushima, kouchi and ehime), we examined haplotypes of mitochondrial dna (mtdna) and genotypes of the nuclear glucosephosphate isomerase-processed pseudogene (gpip) in 189 wild boars. of the 8 different mtdna haplotypes (j5, j10, j12, j13, j15, j19, j20 and e33) detected in shikoku island, the first to be identified were j19 and j20 (of japanese wild boar lineage) and e33 (of europe ... | 2005 | 15997182 |
developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig. | the developmental competence of domestic pig oocytes that were transferred to somatic cell nuclei of miniature pig was examined. a co-culture system of oocytes with follicle shells was used for the maturation of domestic pig oocytes in vitro. co-cultured oocytes progressed to the metaphase ii stage of meiosis more quickly and more synchronously than non co-cultured oocytes. oocytes were enucleated and fused with fibroblast cells of potbelly miniature pig at 48 h of maturation. the blastocyst for ... | 2005 | 15996114 |
trichinae certification in the united states pork industry. | control of trichinella infection in u.s. pork has traditionally been accomplished by inspection of individual carcasses at slaughter or by post-slaughter processing to inactivate parasites. we propose that an alternative to individual carcass testing or processing can be used when pigs are raised in production systems where risk of exposure to trichinella spiralis has been mitigated. declines in prevalence of this parasite in u.s. domestic swine during the last 30 years, coupled with improvement ... | 2005 | 15993000 |
hand-assisted laparoscopic partial nephrectomy in the porcine model using gelatin matrix hemostatic sealant without hilar occlusion. | gelatin matrix hemostatic sealant (gmhs) has been used for hemostasis during partial nephrectomy with hilar clamping. the objective of this study was to determine the ability of gmhs to achieve hemostasis without hilar clamping in the porcine model. | 2005 | 15989447 |
hepatic hemorrhage caused by percutaneous tumor ablation: radiofrequency ablation versus cryoablation in a porcine model. | to determine the extent of hepatic hemorrhage caused by percutaneous cryoablation performed with a small-diameter cryoablation probe compared with that caused by percutaneous radiofrequency (rf) ablation in a porcine model. | 2005 | 15987968 |
[high-resolution t1-weighted mr-lymphography of inguinal lymph nodes after interstitial application of gadomer-17 in animal experiments]. | to evaluate the microstructural anatomy of inguinal lymph nodes in pigs after interstitial mr-lymphography with the dendritic contrast agent gadomer-17. | 2005 | 15973599 |
trichinella britovi etiological agent of sylvatic trichinellosis in the republic of guinea (west africa) and a re-evaluation of geographical distribution for encapsulated species in africa. | in west africa, trichinella infection was documented in humans and animals from senegal in the 1960s, and the biological characters of one isolate showed a lower infectivity to domestic pigs and rodents when compared with that of a trichinella spiralis pig isolate from europe. to identify the trichinella species present in west africa, a survey was conducted in a total of 160 wild animals in the republic of guinea. three viverridae, one true civet (viverra civetta) and two african palm civets (n ... | 2005 | 15964575 |
effects of antemortem ingestion of ethanol on insect successional patterns and development of phormia regina (diptera: calliphoridae). | the effects of antemortem ingestion of ethanol by domestic pigs, sus scrofa l., on postmortem insect successional patterns and the development of phormia regina (meigen) were studied during summer 2003 in blacksburg, va. insect samples were collected from the carcasses of ethanol-treated and untreated pigs for 10 d postmortem during two successional studies. in total, 32 insect taxa were collected during the two studies, with 29 and 27 taxa observed on the carcasses of ethanol-treated and untrea ... | 2005 | 15962802 |
the occurrence of encephalomyocarditis virus (emcv) in european pigs from 1990 to 2001. | the occurrence of encephalomyocarditis virus (emcv) among domestic pigs and wild boar in several european countries is described and discussed. from 1990 to 2001 clinical outbreaks were analysed and serum samples, partly from existing screening programmes, were tested for antibodies against emcv. most clinical emcv outbreaks were reported in belgium (320), followed by italy (110), greece (15) and cyprus (6). the outbreaks appeared to be clustered in 'endemic areas' with an increase in outbreaks ... | 2005 | 15962562 |
inducible nitric oxide synthase inhibition improves intestinal microcirculatory oxygenation and co2 balance during endotoxemia in pigs. | we examined whether selective inhibition of inducible nitric oxide synthase (inos) promotes intestinal microvascular oxygenation (micropo2) and co2 off-load after endotoxic shock. | 2005 | 15959764 |
retrograde renal cooling during radio frequency ablation to protect from renal collecting system injury. | we evaluated the protective effect of continuous retrograde irrigation of ice-cold saline on the renal collecting system during central parenchymal temperature based radio frequency ablation (rfa). | 2005 | 15947688 |
evaluation of a barrier cream against the chemical warfare agent vx using the domestic white pig. | the purpose of this study was to evaluate the efficacy of a novel barrier cream formulation at reducing the percutaneous toxicity of a 2xld(50) liquid challenge of nerve agent (vx). the study was conducted in vitro and in vivo using the domestic pig. pretreatment of the (inner ear skin) exposure site with barrier cream eliminated mortality, reduced cholinesterase inhibition and prevented any physiological or biochemical signs of intoxication. in contrast, untreated animals exposed to vx exhibite ... | 2005 | 15943757 |
influence of ventricular pacing on myocardial oxygen tension. | the influence of heart rate on cardiac output, oxygen consumption, and myocardial activity has been widely investigated. however, the influence of heart rate on myocardial oxygen tension (po2) remains unclear. since the introduction of flexible po2 micro catheters to measure partial oxygen tension in a working muscle, it is possible to investigate the influence of heart rate on myocardial oxygen tension. | 2005 | 15939441 |
a report of decomposition rates of a special burial type in edmonton, alberta from an experimental field study. | regional studies that examine decomposition rates of certain faunal remains can help to determine time since death. forensic anthropologists have long used qualitative decomposition data, but linking these to more quantitative data could improve time since death estimations. experiments were developed in which domestic pigs (sus scrofa) were buried with varying characteristics then excavated and observed over a period of 15 months in edmonton, alberta. data recorded after two weeks, five weeks, ... | 2005 | 15932100 |
macro anatomical investigations of the cranial cervical ganglion in domestic pig (sus scrofa domesticus). | in this study, the left and right cranial cervical ganglia (ganglion cervicale craniale) of eight young (four male, four female) domestic pigs weighing around 70-80 kg were inspected macro anatomically. the cranial cervical ganglion (ccg) was found cranio-ventrally of the distal ganglion of the vagus nerve, medial of the jugular process extremity, ventral of the atlas, dorsal of the epiglottis base and medial of the common root (cr) established by the internal carotid and occipital arteries. the ... | 2005 | 15929737 |
correlation between positivity for immunoglobulin a antibodies and viraemia of swine hepatitis e virus observed among farm pigs in japan. | to evaluate the usefulness of detection of antibodies to hepatitis e virus (hev) to screen for viraemic pigs, serum samples obtained from 1425 1-6-month-old pigs in japan were tested for swine hev rna and igg, igm and iga classes of anti-hev antibody. fifty-five (5 %) of the 1071 2-5-month-old pigs were positive for swine hev rna, but none of 218 1-month-old pigs or 136 6-month-old pigs had detectable hev rna. the prevalence of anti-hev igg among the viraemic pigs (67 %, 37/55) was similar to th ... | 2005 | 15914860 |
[the development of the gene pool in the microevolution of sus scrofa and the role of heterozygosity in the breed-forming process]. | the gene pool formation of the modem domestic breeds of pig sus scrofa domestica and their genesis based on hybridization of wild ancestral forms of the european and asian origin were studied using molecular immunogenetic methods. males of the european and central asian s. scrofa subspecies (s. s. scrofa and s. s. nigripes were hybridized with domestic pigs of the swedish landrace and vietnamese black masked breeds. in addition, we examined the genotypic structure of 65 wild, aboriginal, and loc ... | 2005 | 15909917 |
stent-based delivery of abt-578 via a phosphorylcholine surface coating reduces neointimal formation in the porcine coronary model. | stent-based delivery of the antiproliferative and immunosuppressive macrocyclic lactone sirolimus reduces neointimal formation and restenosis by cytostatic inhibition of vascular smooth muscle cell proliferation. the objective of this study was to determine the feasibility and efficacy of stent-based delivery of abt-578, a structurally unique macrocyclic lactone. stainless steel balloon-expandable stents were coated with thin layer of phosphorylcholine (pc) or pc with abt-578 (10 microg/mm). fif ... | 2005 | 15900559 |
management strategy impacts on ammonia volatilization from swine manure. | ammonia emitted from manure can have detrimental effects on health, environmental quality, and fertilizer value. the objective of this study was to measure the potential for reduction in ammonia volatilization from swine (sus scrofa domestica) manure by temperature control, stirring, addition of nitrogen binder (mohave yucca, yucca schidigera roezl ex ortgies) or urease inhibitor [n-(n-butyl) thiophosphoric triamide (nbpt)], segregation of urine from feces, and ph modification. swine manure [tot ... | 2005 | 15888898 |
a dna vaccine expressing the e2 protein of classical swine fever virus elicits t cell responses that can prime for rapid antibody production and confer total protection upon viral challenge. | immunization of domestic pigs with a dna vaccine expressing the complete e2 protein of classical swine fever virus (csfv) conferred total protection against a severe viral challenge. immunization with three doses of plasmid pcdna3.1/e2 elicited a consistent and specific, mhc class ii restricted t cell response in the three domestic pigs analyzed, in the absence of detectable anti-csfv antibodies in serum. upon challenge specific t cell responses were boosted in the three vaccinated pigs, and a r ... | 2005 | 15882536 |
comparison of male and female meiotic segregation patterns in translocation heterozygotes: a case study in an animal model (sus scrofa domestica l.). | the comparison of male and female meiotic segregation patterns for individuals carrying identical reciprocal translocations has been rarely reported in mammalian species. the main comparative study involving males and females with comparable genetic background has been performed in the mouse. swine is another relevant animal model species for meiotic studies. here we present the segregation patterns determined for sows carrying one of the two following reciprocal translocations: 38, xx, rcp(3;15 ... | 2005 | 15878917 |
transscleral implantation and neurophysiological testing of subretinal polyimide film electrodes in the domestic pig in visual prosthesis development. | loss of photoreceptor function is responsible for a variety of blinding diseases, including retinitis pigmentosa. advances in microtechnology have led to the development of electronic visual prostheses which are currently under investigation for the treatment of human blindness. the design of a subretinal prosthesis requires that the stimulation device should be implantable in the subretinal space of the eye. current limitations in eye surgery have to be overcome to demonstrate the feasibility o ... | 2005 | 15876656 |
foraging behaviour in domestic pigs (sus scrofa): remembering and prioritizing food sites of different value. | this experiment investigated whether domestic pigs can remember the locations of food sites of different relative value, and how a restricted retrieval choice affects their foraging behaviour. nine juvenile female pigs were trained to relocate two food sites out of a possible eight in a spatial memory task. the two baited sites contained different amounts of food and an obstacle was added to the smaller amount to increase handling time. on each trial, a pig searched for the two baited sites (sea ... | 2004 | 15871038 |
experimental study on fluorescent microspheres as a tracer for sentinel node detection. | common tracers for sentinel node navigation surgery are blue dye and technetium-99m-labelled colloids. however, in most esophageal or lung cancer patients, it is impossible to detect the sentinel node among mediastinal nodes by blue dye because of the anthracotic pigmentation of mediastinal nodes. the use of technetium-99m-labelled colloids requires a special facility, while a large hot-spot at the injection site prevents detection of the sentinel node around the primary lesion. to overcome thes ... | 2005 | 15868914 |
impedance-gradient electrode reduces skin irritation induced by transthoracic defibrillation. | a new type of disposable external defibrillation electrode has been developed to reduce the skin irritation commonly associated with defibrillation and synchronised cardioversion. this design employs an impedance gradient to reduce the proportion of current delivered to the electrode periphery. the temperature distribution under the new electrode was compared with that of four other types of commercially available electrodes after repeated high-energy biphasic defibrillation discharges to domest ... | 2005 | 15865132 |
assessment of in vivo mr imaging compared to physical sections in vitro--a quantitative study of brain volumes using stereology. | the object of the present study was to compare stereological estimates of brain volumes obtained in vivo by magnetic resonance imaging (mri) to corresponding volumes from physical sections in vitro. brains of ten domestic pigs were imaged using a 3-t scanner. the volumes of different brain compartments were obtained from mr images by two observers and from physical sections using the cavalieri estimator in combination with point counting. paired t tests revealed no significant differences betwee ... | 2005 | 15862205 |
an investigation into natural resistance to african swine fever in domestic pigs from an endemic area in southern africa. | a population of domestic pigs in northern mozambique with increased resistance to the pathogenic effects of african swine fever (asf) virus was identified by the high prevalence of circulating antibodies to asf virus. an attempt was made to establish whether the resistance in this population was heritable. some of these pigs were acquired and transported to a quarantine facility and allowed to breed naturally. offspring of the resistant pigs were transferred to a high security facility where the ... | 2004 | 15861893 |
phylogenetic analysis of classical swine fever virus (csfv) field isolates from outbreaks in south and central america. | to date, there is little information concerning the epidemiological situation of classical swine fever (csf) in the americas. besides summarizing the available data, genotyping of isolates from outbreaks in domestic pigs in several countries of south and central america was performed. for this, a 190 base fragment of the e2 envelope glycoprotein gene was used. european strains and isolates, and historical isolates from the united states (us) were included for comparison. in contrast to the situa ... | 2005 | 15845261 |
optimization of a blood pool contrast agent injection protocol for mr angiography. | to design an ideal first-pass profile for mr angiography (mra) by optimizing a multiphasic injection protocol based on two experimental animal models. | 2005 | 15834909 |
increases in both buccal and sublingual partial pressure of carbon dioxide reflect decreases of tissue blood flows in a porcine model during hemorrhagic shock. | earlier studies had demonstrated that gastric partial pressure of carbon dioxide (pco2) of the gastric wall is an early indication of perfusion failure. because hypercarbia is a general phenomenon of perfusion failure, this study investigated whether sublingual and buccal mucosal tissue pco2 also serve as a sensitive indicator of systemic blood flow during hemorrhagic shock. | 2005 | 15824661 |
chromosome restriction enzyme digestion in domestic pig (sus scrofa) constitutive heterochromatin arrangement. | the bimodal karyotype of pig appears to contain two types of constitutive heterochromatin, reflecting different satellite dna families: gc-rich heterochromatin located mainly in the centromeric regions of the biarmed chromosomes, and less-gc-rich heterochromatin in the centromeric regions of the one-armed chromosomes. in order to better discriminate this constitutive heterochromatin, we treated pig chromosome preparations with eight different restriction endonucleases, followed by c-banding. thi ... | 2005 | 15824456 |
experimental hypercholesterolemia differentially affects adventitial vasa vasorum and vessel structure of the left internal thoracic and coronary arteries. | atherosclerosis is a chronic and diffuse disease that affects all vascular beds. however, some vascular beds are more prone to atherosclerosis than others. recent evidence suggests a role for the vasa vasorum in the atherosclerotic process. we hypothesized that there is a difference in adventitial vasa vasorum structure between the left internal thoracic artery and the coronary artery. hence the current study was designed to characterize and compare the structure of the adventitial vasa vasorum ... | 2005 | 15821642 |
successional behavior and occurrence matrix of carrion-associated arthropods in the urban area of medellín, colombia. | forensic entomology is a scientific tool applied to the study of insect or arthropod succession at the scene of a crime or that associated with an accident or natural death. interpreting this succession provides information to determine minimum and maximum limits of the postmortem interval (pmi), that is, the time between death and the discovery of the body. this study was carried out during the rainy season, from 27 october to 12 december 2002 in an urban area of the city of medellín, colombia. ... | 2005 | 15813558 |
the effect of age on the morphometric sperm traits of domestic pigs (sus scrofa domestica). | the experiment was conducted using 20 male domestic pigs, which were in 2 (equal-sized) age groups: under 14 months old and over 18 months old. at least 5 ejaculates from each male were taken, and in each ejaculate, morphometric measurements of 50 spermatozoa were made. the measured parameters were head area, head length and width, and flagellum length. for each ejaculate, the basic physical traits and the frequency of occurrence of developmental anomalies of the spermatozoa were examined. it wa ... | 2005 | 15809675 |
hepatic function in hypothermically stored porcine livers: comparison of hypothermic machine perfusion vs cold storage. | hypothermic machine perfusion (hmp) has a potential to relieve the current donor liver crisis by providing an improved and extended preservation method. this study examined the effect of hmp on hepatocellular functions, using a prototype liver transporter capable of preserving livers for 24 hours. livers obtained from adult farm pigs (28 to 32 kg body weight) were divided into three groups: fresh control, hmp, and simple cold storage (n = 4 each). a 4-hour normothermic reperfusion of livers was ... | 2005 | 15808637 |
nerve monitoring and stimulation during endoscopic neck surgery in the pig. | to determine the feasibility of recurrent laryngeal nerve monitoring and stimulation during endoscopic neck surgery in an animal model. | 2005 | 15805886 |
polyclonal hypergammaglobulinemia and formation of hydrophobic immune complexes in porcine reproductive and respiratory syndrome virus-infected and uninfected pigs. | infection of young conventional, domestic pigs with porcine reproductive and respiratory syndrome virus (prrsv) strains vr2332 and ja142 resulted in a rapid, progressive increase in serum igg reaching maximum levels of 20-30 mg/ml at about 3 weeks post infection (p.i.), which were maintained until at least 63 days p.i., whereas the level of serum igg remained at 4-6 mg/ml in sham infected pigs. in most of the vr2332 and ja142-infected pigs hypergammaglobulimenia was associated with the formation ... | 2005 | 15802958 |
smooth-muscle regeneration after electrosurgical endopyelotomy in a porcine model as confirmed by electron microscopy. | endopyelotomy is the preferred treatment for ureteropelvic junction (upj) obstruction because of its short operating time, limited morbidity, fast recovery, and reasonable efficacy. we used tissue and immunohistochemistry staining and electron microscopy to look at the muscle regeneration following an endopyelotomy incision in a porcine model. | 2004 | 15801366 |
adrenergic stimulation of rb+ uptake in pig hearts in vivo assessed by 87rb mrs. | it was previously shown that the adrenergic agonist dobutamine stimulates rb(+) uptake in isolated pig hearts. in the present work we examined whether dobutamine can increase rb(+) uptake rate in vivo. open-chest domestic pigs (n = 14) were used under general anesthesia. the surface coil was placed against the anterior left ventricular wall to obtain (87)rb spectra using a spectrometer interfaced with the 7t, 30-cm horizontal bore magnet. rbcl was infused at the rate of 1.35 +/- 0.14 mmol/kg/hr ... | 2005 | 15799046 |
laparoscopic ureterocalicostomy: development of a technique simplified by application of nitinol clips and a wet monopolar electrosurgery device. | we developed a technique for laparoscopic ureterocalicostomy with the use of intracorporeal suturing and subsequently simplified the technique by application of experimental nitinol clips. | 2005 | 15798423 |
impact of a double-pigtail stent on ureteral peristalsis in the porcine model: initial studies using a novel implantable magnetic sensor. | the effect of stents on ureteral peristalsis in vivo is not entirely clear. we sought to develop a minimally invasive method for its study. | 2005 | 15798413 |
the new selective i(ks)-blocking agent hmr 1556 restores sinus rhythm and prevents heart failure in pigs with persistent atrial fibrillation. | antiarrhythmic drugs for treatment of atrial fibrillation in patients with heart failure are limited by proarrhythmia and low efficacy. experimental studies indicate that the pure i(ks) blocking agents chromanol 293b and hmr 1556 prolong repolarization more markedly at fast than at slow heart rates and during beta-adrenergic stimulation. these properties may overcome some of the above quoted limitations. | 2005 | 15795798 |
anatomical study on true hermaphroditism in an indian pig (sus scrofa domesticus). | a pig was confirmed to be a true hermaphrodite on the basis of gross and histomorphological studies of the genital organs. the genitalia was consisted of left ovary, oviduct, two coiled uterine horns, body of uterus along with right testis and an epididymis. vagina and vulva were absent but male urethra with prostate gland was present. grossly the size of all the genital organs appeared to be normal. histomorphologically, testis and epididymis were underdeveloped as there was no clearcut spermat ... | 2005 | 15785130 |
elephants and human color-blind deuteranopes have identical sets of visual pigments. | being the largest land mammals, elephants have very few natural enemies and are active during both day and night. compared with those of diurnal and nocturnal animals, the eyes of elephants and other arrhythmic species, such as many ungulates and large carnivores, must function in both the bright light of day and dim light of night. despite their fundamental importance, the roles of photosensitive molecules, visual pigments, in arrhythmic vision are not well understood. here we report that eleph ... | 2005 | 15781694 |
comparison of external catheters with subcutaneous vascular access ports for chronic vascular access in a porcine model. | we sought to compare the outcomes of two chronic vascular access techniques, the externalized catheter and the subcutaneous vascular access port, in pigs. female farm pigs (n = 30) underwent placement of a chronic vascular access device in the jugular vein for a research protocol: 18 of the animals underwent placement of a tunneled hickman catheter (thc), and the remaining 12 animals underwent placement of a subcutaneous vascular access port (vap) without external components. after placement of ... | 2005 | 15773772 |
worldwide phylogeography of wild boar reveals multiple centers of pig domestication. | mitochondrial dna (mtdna) sequences from 686 wild and domestic pig specimens place the origin of wild boar in island southeast asia (isea), where they dispersed across eurasia. previous morphological and genetic evidence suggested pig domestication took place in a limited number of locations (principally the near east and far east). in contrast, new genetic data reveal multiple centers of domestication across eurasia and that european, rather than near eastern, wild boar are the principal source ... | 2005 | 15761152 |
comparison between dobutamine and levosimendan for management of postresuscitation myocardial dysfunction. | to investigate the effects of levosimendan, a nonadrenergic inotropic calcium sensitizer, in comparison with adrenergic dobutamine for the management of postresuscitation myocardial dysfunction following resuscitation from prolonged cardiac arrest. | 2005 | 15753736 |
smooth muscle cell proliferation index correlates with 111in-labeled antibody z2d3 uptake in a transplant vasculopathy swine model. | transplant vasculopathy is a major cause of morbidity and mortality in heart transplantation. the proliferation of coronary vascular smooth muscle cells is a hallmark of transplant vasculopathy. the goal of this study was to detect coronary vascular smooth muscle cell proliferation in a swine model by imaging regions of uptake of a monoclonal antibody (z2d3) labeled with 111in. | 2005 | 15750167 |