Publications

midgut transcriptome of the cockroach periplaneta americana and its microbiota: digestion, detoxification and oxidative stress response.	the cockroach, periplaneta americana, is an obnoxious and notorious pest of the world, with a strong ability to adapt to a variety of complex environments. however, the molecular mechanism of this adaptability is mostly unknown. in this study, the genes and microbiota composition associated with the adaptation mechanism were studied by analyzing the transcriptome and 16s rdna pyrosequencing of the p. americana midgut, respectively. midgut transcriptome analysis identified 82,905 unigenes, among ...	2016	27153200
inhibition and larvicidal activity of phenylpropanoids from piper sarmentosum on acetylcholinesterase against mosquito vectors and their binding mode of interaction.	aedes aegypti, aedes albopictus and culex quinquefasciatus are vectors of dengue fever and west nile virus diseases. this study was conducted to determine the toxicity, mechanism of action and the binding interaction of three active phenylpropanoids from piper sarmentosum (piperaceae) toward late 3rd or early 4th larvae of above vectors. a bioassay guided-fractionation on the hexane extract from the roots of piper sarmentosum led to the isolation and identification of three active phenylpropanoi ...	2016	27152416
management and treatment of dengue and chikungunya - natural products to the rescue.	neglected tropical diseases (ntds) flourish mostly in impoverished developing nations of the world. it is estimated that ntds plague up to 1 billion people every year thereby inducing a massive economic and health burden worldwide. following explosive outbreaks mostly in asia, latin america, europe and the indian ocean, two common ntds namely, chikungunya and dengue both transmitted by an infected mosquito vector principally aedes aegypti have emerged as a major public health threat. given the l ...	2016	27151484
δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes.	mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ...	2016	27151483
zika virus: a new animal model for an arbovirus.		2016	27149628
zika virus: the latest newcomer.	since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ...	2016	27148186
public health round-up.		2016	27147758
a mosquito sperm's journey from male ejaculate to egg: mechanisms, molecules, and methods for exploration.	the fate of mosquito sperm in the female reproductive tract has been addressed sporadically and incompletely, resulting in significant gaps in our understanding of sperm-female interactions that ultimately lead to fertilization. as with other diptera, mosquito sperm have a complex journey to their ultimate destination, the egg. after copulation, sperm spend a short time at the site of insemination where they are hyperactivated and quickly congregate near the entrance of the spermathecal ducts. w ...	0	27147424
hawkmoths evaluate scenting flowers with the tip of their proboscis.	pollination by insects is essential to many ecosystems. previously, we have shown that floral scent is important to mediate pollen transfer between plants (kessler et al., 2015). yet, the mechanisms by which pollinators evaluate volatiles of single flowers remained unclear. here, nicotiana attenuata plants, in which floral volatiles have been genetically silenced and its hawkmoth pollinator, manduca sexta, were used in semi-natural tent and wind-tunnel assays to explore the function of floral sc ...	2016	27146894
ionotropic chemosensory receptors mediate the taste and smell of polyamines.	the ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. polyamine deficiency or excess has detrimental effects on health, cognitive function, reproduction, and lifespan. here, we show that a diet high in polyamine is beneficial and increases reproductive success of flies, and ...	2016	27145030
vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus.	from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito.	2016	27144888
imbalanced hemolymph lipid levels affect feeding motivation in the two-spotted cricket, gryllus bimaculatus.	insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. adipokinetic hormone (akh) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. we recently demonstrated that rna interference (rnai)-mediated knockdown of the akh receptor (akhr) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, gr ...	2016	27144650
chikungunya fever cases identified in the veterans health administration system, 2014.	during december 2013, the first locally transmitted chikungunya virus (chikv) infections in the americas were reported in the caribbean. although chikv infection is rarely fatal, risk for severe disease increases with age and medical comorbidities. herein we describe characteristics of veterans health administration (vha) patients with chikv infection and, among those with infections diagnosed in puerto rico, investigated risk factors for hospitalization.	2016	27144588
spatial and temporal country-wide survey of temephos resistance in brazilian populations of aedes aegypti.	the organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (aedes aegypti) in brazil since the mid-1980s. reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. as resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in ae. aegypti in br ...	2016	27143489
overview of zika virus (zikv) infection in regards to the brazilian epidemic.	zika virus (zikv), a mosquito-borne flavivirus, belongs to the flaviviridae family, genus flavivirus. zikv was initially isolated in 1947 from a sentinel monkey in the zika forest, uganda. little clinical importance was attributed to zikv, once only few symptomatic cases were reported in some african and southeast asiatic countries. this situation changed in 2007, when a large outbreak was registered on the yap island, micronesia, caused by the asian zikv lineage. between 2013 and 2014, zikv spr ...	2016	27143174
optimal repellent usage to combat dengue fever.	dengue fever is one of the most important vector-borne diseases. it is transmitted by aedes stegomyia aegypti, and one of the most effective strategies to combat the disease is the reduction of exposure to bites of these mosquitoes. in this paper, we present a game-theoretical model in which individuals choose their own level of protection against mosquito bites in order to maximize their own benefits, effectively balancing the cost of protection and the risk of contracting the dengue fever. we ...	2016	27142427
differential gene expression analysis of the epacromius coerulipes (orthoptera: acrididae) transcriptome.	epacromius coerulipes (ivanov) is one of the most widely distributed locusts. to date, the main methods to kill locusts still rely on chemical controls, which can result in the selection of locusts with resistance to chemical pesticides. butene-fipronil is a new pesticide that was discovered by the structural modification of fipronil. this pesticide has been used to control various agricultural pests and has become an important pesticide product to control pests that exhibit resistance to other ...	2016	27142308
impact of inter- and intra-specific competition among larvae on larval, adult, and life-table traits of aedes aegypti and aedes albopictus females.	few studies have taken a comprehensive approach of measuring the impact of inter- and intra-specific larval competition on adult mosquito traits. in this study, the impact of competition aedes aegypti and a. albopictus was quantified over the entire life of a cohort.competitive treatments affected hatch-to-adult survivorship and development time to adulthood of females for both species, but affected median wing length of females only for a. albopictus. competitive treatments had no significant e ...	2016	27141149
molecular characterization of two major dengue outbreaks in costa rica.	dengue virus (denv) (flavivirus, flaviviridae) is a reemerging arthropod-borne virus with a worldwide circulation, transmitted mainly by aedes aegypti and aedes albopictus mosquitoes. since the first detection of its main transmitting vector in 1992 and the invasion of denv-1 in 1993, costa rica has faced dengue outbreaks yearly. in 2007 and 2013, costa rica experienced two of the largest outbreaks in terms of total and severe cases. to provide genetic information about the etiologic agents prod ...	2016	27139442
identification and characterization of two novel rna viruses from anopheles gambiae species complex mosquitoes.	mosquitoes of the anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in africa. however, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of o'nyong-nyong virus, closely related to chikungunya virus. deep-sequencing has revealed different rna viruses in natural insect viromes, but none have been previously described in the anopheles gambiae species complex. here, we d ...	2016	27138938
sequence conservation and sexually dimorphic expression of the ftz-f1 gene in the crustacean daphnia magna.	identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. orthologs of drosophila orphan receptor fushi tarazu factor-1 (ftz-f1) are known to function in genetic sex determination. in contrast, their roles in environmental sex determination remain unknown. in this study, we have cloned and characterized the ftz-f1 ortholog in the branchiopod crustacean daphnia magna, which produces males in ...	2016	27138373
the p450 cyp6z1 confers carbamate/pyrethroid cross-resistance in a major african malaria vector beside a novel carbamate-insensitive n485i acetylcholinesterase-1 mutation.	carbamates are increasingly used for vector control notably in areas with pyrethroid resistance. however, a cross-resistance between these insecticides in major malaria vectors such as anopheles funestus could severely limit available resistance management options. unfortunately, the molecular basis of such cross-resistance remains uncharacterized in an. funestus, preventing effective resistance management. here, using a genomewide transcription profiling, we revealed that metabolic resistance t ...	2016	27135886
molecular characterisation of clinical isolates of chikungunya virus: a study from tertiary care hospitals in southern india.	indian ocean islands and india have experienced massive severe chikungunya outbreak from 2005 up till now and then chikungunya became epidemic in india. the mutations that occurred in e1 gene were responsible for increased infectivity, virulence and host adaptability. it is important to find out the genotype and its probable evolvement and novel mutations in the e1 gene reported during 2006-2009 from the current isolates, which may affect the local protein structure.	2016	27134872
zika virus infections imported from brazil to portugal, 2015.	zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ...	2016	27134823
first identification of kdr allele f1534s in vgsc gene and its association with resistance to pyrethroid insecticides in aedes albopictus populations from haikou city, hainan island, china.	aedes albopictus is distributed widely in china, as a primary vector of dengue fever and chikungunya fever in south of china. chemical insecticide control is one of the integrated programmes to prevent mosquito-borne diseases. long-term applications of pyrethroids have resulted in the development of resistance in ae. albopictus populations in china. however, the susceptibility of ae. albopictus to pyrethroids in hainan island was unclear. knockdown resistance (kdr), caused by point mutations in ...	2016	27133234
dengue virus immunopathogenesis: lessons applicable to the emergence of zika virus.	dengue is the leading mosquito-transmitted viral infection in the world. there are more than 390 million new infections annually; while the majority of infected individuals are asymptomatic or develop a self-limited dengue fever, up to 1 million clinical cases develop severe manifestations, including dengue hemorrhagic fever and shock syndrome, resulting in ~25,000 deaths annually, mainly in children. gaps in our understanding of the mechanisms that contribute to dengue infection and immunopatho ...	2016	27130436
multiple qtl determine dorsal abdominal scale patterns in the mosquito aedes aegypti.	the mosquito, aedes aegypti (l.) originated in sub-saharan africa as a dark form sylvan species (a. aegypti formosus). evolution of a. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. we investigated the genetic basis for abdominal white scale presence that represents the diagnostic for sylvan a. aegypti formosus (scales absent), from type form (scales present) and a. aegypti queenslandensis form (dense scaling). we performed qu ...	2016	27130203
erratum to: co-distribution and co-infection of chikungunya and dengue viruses.		2016	27129475
the risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in rio de janeiro, brazil.	rio de janeiro in brazil will host the summer olympic games in 2016. about 400,000 non-immune foreign tourists are expected to attend the games. as brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified.	2016	27129407
how relevant is panallergen sensitization in the development of allergies?	panallergens comprise various protein families of plant as well as animal origin and are responsible for wide ige cross-reactivity between related and unrelated allergenic sources. such cross-reactivities include reactions between various pollen sources, pollen and plant-derived foods as well as invertebrate-derived inhalants and foodstuff. here, we provide an overview on the most clinically relevant panallergens from plants (profilins, polcalcins, non-specific lipid transfer proteins, pathogene ...	0	27129102
predicting dengue fever outbreaks in french guiana using climate indicators.	dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. associations between climate and dengue have been studied around the world, but the results have shown that the impact of the climate can vary widely from one study site to another. in french guiana, climate-based models are not available to assist in developing an early warning system. this study aims to evaluate the potential of using oceanic and atmospheric conditions to help predict dengue f ...	2016	27128312
phytochemical composition, antifungal and antioxidant activity of duguetia furfuracea a. st.-hill.	background. duguetia furfuracea is popular plant used in popular medicine. hypothesis/purpose. this claim evaluated the phytochemical composition of the hydroethanolic extract (hedf), fractions of duguetia furfuracea, and antioxidant and antifungal activity. methods. the chemical profile was carried out by hplc-dad. the total phenolic contents and flavonoid components were determined by folin-ciocalteu and aluminium chloride reaction. the antioxidant activity was measured by scavenging of 2,2-di ...	2016	27127550
the wmel strain of wolbachia reduces transmission of chikungunya virus in aedes aegypti.	new approaches to preventing chikungunya virus (chikv) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. a promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium wolbachia into aedes aegypti mosquitoes. this primarily has been proposed as a tool to control dengue viru ...	2016	27124663
zika virus: an update on epidemiology, pathology, molecular biology, and animal model.	zika virus (zikv) was first described in 1947, and became a health emergency problem in 2016 when its association with fetal microcephaly cases was confirmed by centers for disease control and prevention (cdc) in the united states. to date, zikv infection has been documented in 66 countries. zikv is recognized as a neurotropic virus and numerous diseases manifested in multiple neurological disorders have been described, mainly in countries that have been exposed to zikv after the 2007 outbreak i ...	2016	27124623
analogs of ldl receptor ligand motifs in dengue envelope and capsid proteins as potential codes for cell entry.	it is established that cell entry of low density lipoprotein particles (llps) containing apo b100 and apo e is mediated by receptors and gags. receptor ligand motifs, xbbbxxbx, xbbxbx, and ψbψxb, and mono- and bipartite nls sequences are abundant in apo e and apo b100 as well as in envelope and capsid proteins of dengue viruses 1-4 (denv1-4). synthetic, fluorescence-labeled peptides of sequences in denv2 envelope protein, and denv3 capsid that include these motifs were used to conduct a qualitat ...	0	27123468
international air travel to ohio, usa, and the impact on malaria, influenza, and hepatitis a.	the state of ohio led the united states in measles in 2014, ostensibly related to international air travel (iat), and ranked lower than 43 other states in infectious disease outbreak preparedness. we conducted a retrospective cohort study using surveillance data of the total ohio population of 11 million from 2010 through 2014 with a nested case control of air travelers to determine the risk of malaria, seasonal influenza hospitalizations (ih), and hepatitis a (ha) disease related to internation ...	2016	27123365
rhodnius prolixus and r. robustus (hemiptera: reduviidae) nymphs show different locomotor patterns on an automated recording system.	circadian rhythms of triatomines, vectors of the etiological agent trypanosoma cruzi responsible for chagas disease, have been extensively studied in adults of the two most epidemiologically relevant vector species, rhodnius prolixus and triatoma infestans. however, little attention has been dedicated to the activity patterns in earlier developmental stages, even though triatomine nymphs are equally capable of transmitting t. cruzi to humans. because circadian rhythms may differ even between clo ...	2016	27121502
de novo adult transcriptomes of two european brittle stars: spotlight on opsin-based photoreception.	next generation sequencing (ngs) technology allows to obtain a deeper and more complete view of transcriptomes. for non-model or emerging model marine organisms, ngs technologies offer a great opportunity for rapid access to genetic information. in this study, paired-end illumina hiseqtm technology has been employed to analyse transcriptomes from the arm tissues of two european brittle star species, amphiura filiformis and ophiopsila aranea. about 48 million illumina reads were generated and 136 ...	2016	27119739
excretion of dengue virus rna by aedes aegypti allows non-destructive monitoring of viral dissemination in individual mosquitoes.	successful transmission of a vector-borne pathogen relies on a complex life cycle in the arthropod vector that requires initial infection of the digestive tract followed by systemic viral dissemination. the time interval between acquisition and subsequent transmission of the pathogen, called the extrinsic incubation period, is one of the most influential parameters of vector-borne pathogen transmission. however, the dynamic nature of this process is often ignored because vector competence assays ...	2016	27117953
the larvicidal effects of black pepper (piper nigrum l.) and piperine against insecticide resistant and susceptible strains of anopheles malaria vector mosquitoes.	insecticide resistance carries the potential to undermine the efficacy of insecticide based malaria vector control strategies. therefore, there is an urgent need for new insecticidal compounds. black pepper (dried fruit from the vine, piper nigrum), used as a food additive and spice, and its principal alkaloid piperine, have previously been shown to have larvicidal properties. the aim of this study was to investigate the larvicidal effects of ground black pepper and piperine against third and fo ...	2016	27117913
droplet characterization and penetration of an ultra-low volume mosquito adulticide spray targeting the asian tiger mosquito, aedes albopictus, within urban and suburban environments of northeastern usa.	adult control of aedes albopictus via ultra-low volume is difficult because this species occurs primarily in peridomestic habitats where obstacles such as buildings and vegetation can disrupt spray plumes and droplet dispersion. we determined droplet penetration and characterization of a pyrethroid adulticide applied from the ground at mid (46.77 ml/ha) and maximum (93.53 ml/ha) label rates within cryptic habitats of urban and suburban environments. droplets were collected from all habitats, wit ...	2016	27116103
production of dengue virus envelope protein domain iii-based antigens in tobacco chloroplasts using inducible and constitutive expression systems.	dengue fever is a disease in many parts of the tropics and subtropics and about half the world's population is at risk of infection according to the world health organization. dengue is caused by any of the four related dengue virus serotypes den-1, -2, -3 and -4, which are transmitted to people by aedes aegypti mosquitoes. currently there is only one vaccine (dengvaxia(®)) available (limited to a few countries) on the market since 2015 after half a century's intensive efforts. affordable and ac ...	2016	27116001
spread of the invasive mosquitoes aedes aegypti and aedes albopictus in the black sea region increases risk of chikungunya, dengue, and zika outbreaks in europe.		2016	27115737
zika virus: a global threat to humanity: a comprehensive review and current developments.	at present, one of greatest concerns of medical personnel is zika virus (zikv). though it has been reported for quite a long time, its rapid emergence, new modes of transmission, and more importantly, the congenital anomalies associated with it have made the situation worse. it was first detected in 1947. after that, this infection was found in the countries of africa as well as asia. at present, interestingly it has been reported from brazil. microcephaly and intracranial calcification have bee ...	0	27114968
characterization of drosophila cmp-sialic acid synthetase activity reveals unusual enzymatic properties.	cmp-sialic acid synthetase (csas) is a key enzyme of the sialylation pathway. csas produces the activated sugar donor, cmp-sialic acid, which serves as a substrate for sialyltransferases to modify glycan termini with sialic acid. unlike other animal csass that normally localize in the nucleus, drosophila melanogaster csas (dmcsas) localizes in the cell secretory compartment, predominantly in the golgi, which suggests that this enzyme has properties distinct from those of its vertebrate counterpa ...	2016	27114558
pfsmad4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster pinctada fucata.	mollusca is the second largest phylum in nature. the shell of molluscs is a remarkable example of a natural composite biomaterial. biomineralization and how it affects mollusks is a popular research topic. the bmp-2 signaling pathway plays a canonical role in biomineralization. smad4 is an intracellular transmitter in the bmp signaling pathway in mammals, and some genomic data show smad4's involvement in bmp signaling in invertebrates, but whether smad4 plays a conservative role in pearl oyster, ...	2016	27113217
insights into the evolution of chemoreceptor genes superfamily in tyrophagus putrescentiae (acari: acaridae).	all living organisms, including animals, plants, fungi, and bacteria, use the olfactory system to recognize chemicals or pheromone from their environment. insects detect a volatile substance using odorant receptors (ors) or gustatory receptors (grs) and ionotropic receptors (irs). the gene families of the olfactory system in acari are still not clear. in this study, we identified seven ors, one gr, and five irs from the transcriptome of the storage mite, tyrophagus putrescentiae schrank. no olfa ...	2016	27113112
detection and distribution of v1016ikdr mutation in the voltage-gated sodium channel gene in aedes aegypti (diptera: culicidae) populations from sergipe state, northeast brazil.	aedes aegypti (l.) resistance to pyrethroids was recorded in brazil few years after its introduction as the adulticide in the national dengue control program campaigns. altered susceptibility to pyrethroids had been reported in the state of sergipe, northeast brazil, through biological assays, even before its use against ae. aegypti in the state. metabolic and target-site resistance mechanisms were also revealed in samples from aracaju, the capital of sergipe. herein, we investigated the presenc ...	2016	27113110
recent changes in the local distribution of aedes aegypti (diptera: culicidae) in south florida, usa.	disease transmission is directly tied to the spatial distribution of disease vectors. the distribution of aedes aegypti (l.) in the united states has diminished since the introduction of aedes albopictus (skuse) in the 1980s. however, ae. aegypti persists in some urban areas, particularly in south florida. the pattern of habitat segregation of these two species is well documented, but the consistency of this phenomenon over time is unknown. to examine the dynamics of the local distributions of t ...	2016	27113103
prevalence of dengue and chikungunya virus infections in north-eastern tanzania: a cross sectional study among participants presenting with malaria-like symptoms.	in spite of increasing reports of dengue and chikungunya activity in tanzania, limited research has been done to document the general epidemiology of dengue and chikungunya in the country. this study aimed at determining the sero-prevalence and prevalence of acute infections of dengue and chikungunya virus among participants presenting with malaria-like symptoms (fever, headache, rash, vomit, and joint pain) in three communities with distinct ecologies of north-eastern tanzania.	2016	27112553
knowledge, attitudes, and practices of florida physicians regarding dengue before and after an educational intervention.	failure to recognize and appropriately manage dengue early in the clinical course may result in late initiation of supportive treatment for severe disease. in florida, travel-related and autochthonous dengue occur and are likely under-recognized. the objective of this study was to evaluate physician knowledge of dengue and its management before and after an educational intervention in florida.	2016	27112138
analysis of protein composition and bioactivity of neoponera villosa venom (hymenoptera: formicidae).	ants cause a series of accidents involving humans. such accidents generate different reactions in the body, ranging from a mild irritation at the bite site to anaphylactic shock, and these reactions depend on the mechanism of action of the venom. the study of animal venom is a science known as venomics. through venomics, the composition of the venom of several ant species has already been characterized and their biological activities described. thus, the aim of this study was to evaluate the pro ...	2016	27110765
vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes.	the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ...	2016	27108077
effects of larval nutrition on wolbachia-based dengue virus interference in aedes aegypti (diptera: culicidae).	in order to assess the broad-scale applicability of field releases of wolbachia for the biological control of insect-transmitted diseases, we determined the relationship between the larval diet of aedes aegypti l. mosquitoes infected with wolbachia strains and their susceptibility to dengue virus (denv) infection via intrathoracic injection and oral inoculation. larvae were reared on diets that varied in the quantity of food which had the effect of modifying development time and adult body size. ...	2016	27106932
seasonality and the effectiveness of mass vaccination.	many infectious diseases have seasonal outbreaks, which may be driven by cyclical environmental conditions (e.g., an annual rainy season) or human behavior (e.g., school calendars or seasonal migration). if a pathogen is only transmissible for a limited period of time each year, then seasonal outbreaks could infect fewer individuals than expected given the pathogen's in-season transmissibility. influenza, with its short serial interval and long season, probably spreads throughout a population un ...	0	27105983
sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms.	the recent expansion of next-generation sequencing has significantly improved biological research. nevertheless, deep exploration of genomes or metagenomic samples remains difficult because of the sequencing depth and the associated costs required. therefore, different partitioning strategies have been developed to sequence informative subsets of studied genomes. among these strategies, hybridization capture has proven to be an innovative and efficient tool for targeting and enriching specific b ...	2016	27105841
development of a sybr green i-based quantitative rt-pcr for ross river virus: application in vector competence studies and antiviral drug evaluation.	ross river virus (rrv) is an emerging alphavirus and is presently endemic in many parts of oceania. keeping in mind its emergence, we developed a molecular detection system and utilized it to study vector competence and evaluate activity of antiviral compounds against rrv. a sybr green i-based quantitative rt-pcr for detection of rrv was developed targeting the e2 gene, with a detection limit of 100 rna copies/reaction. the specificity was confirmed with closely related alphaviruses and flavivir ...	2016	27105737
inferring the spatio-temporal patterns of dengue transmission from surveillance data in guangzhou, china.	dengue is a serious vector-borne disease, and incidence rates have significantly increased during the past few years, particularly in 2014 in guangzhou. the current situation is more complicated, due to various factors such as climate warming, urbanization, population increase, and human mobility. the purpose of this study is to detect dengue transmission patterns and identify the disease dispersion dynamics in guangzhou, china.	2016	27105350
reproductive incompatibility involving senegalese aedes aegypti (l) is associated with chromosome rearrangements.	aedes aegypti, the primary vector of dengue, yellow fever and zika flaviviruses, consists of at least two subspecies. aedes aegypti (aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. aedes aegypti formosus (aaf), has a dark cuticle, is restricted to sub-saharan africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. scale patterns correlate with cuticle ...	2016	27105225
characteristics and efficacy of three commercial handheld thermal foggers with pyrethroid insecticides against three species of mosquitoes.	the field study's objectives were to compare the physical characteristics as well as efficacy with multiple insecticides for bonide(®) fog rx insect fogger, black flag(®) propane insect fogger, and burgess(®) outdoor propane insect fogger. evaluations were conducted with 7 machine chemical combinations, 3 depths of spray, and 3 species of laboratory-reared mosquitoes, anopheles quadrimaculatus, culex quinquefasciatus, and aedes aegypti. combinations of these factors were analyzed in conjunction ...	2016	27105215
age-stage, two-sex life table characteristics of aedes albopictus and aedes aegypti in penang island, malaysia.	the life table developmental attributes of laboratory colonies of wild strains of aedes albopictus and aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (r0), and mean generation time (t). the total preadult development time was 9.47 days for ae. albopictus and 8.76 days fo ...	2016	27105211
infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains.	chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ...	2016	27102548
enhancement of aedes albopictus collections by ovitrap and sticky adult trap.	in the last decades, aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which dengue, chikungunya and zika. enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control ...	2016	27102015
an updated checklist of mosquito species (diptera: culicidae) from madagascar.	an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ...	2016	27101839
epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama.	neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ...	2016	27101567
the mitotic spindle: linking teratogenic effects of zika virus with human genetics?	recently, an association between zika virus infection and microcephaly/ocular findings was found to be reasonable e.g. because of the demonstration that the virus was found in the brain of a fetus after presumed maternal infection. although there is no proof yet for a causal relationship, for an appropriate risk calculation efforts are urgently needed to either establish or disprove this assumption.	2016	27099632
no evidence for local adaptation of dengue viruses to mosquito vector populations in thailand.	despite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. fine-scale genetic structure of mosquito vector populations and evidence for genotype × genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. to test this hypo ...	2016	27099625
depletion of alveolar macrophages does not prevent hantavirus disease pathogenesis in golden syrian hamsters.	andes virus (andv) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (hps). the mechanism for the massive vascular leakage associated with hps is poorly understood; however, dysregulation of components of the immune response is often suggested as a possible cause. alveolar macrophages are found in the alveoli of the lung and represent the first line of defense to many airborne pathogens. to determine whether alveolar macrophages play a role in hps ...	2016	27099308
development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses.	dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ...	2016	27098955
the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania.	lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ...	2016	27098567
association between nutritional status and dengue infection: a systematic review and meta-analysis.	dengue infection has various clinical manifestations, often with unpredictable clinical evolutions and outcomes. several factors including nutritional status have been studied to find the relationship with dengue severity. however, the nutritional status had conflicting effects on the complication of dengue in some previous studies. therefore, we conducted a systematic review and performed a meta-analysis to analyze the association between nutritional status and the outcome of dengue infection.	2016	27097934
in vitro template-change pcr to create single crossover libraries: a case study with b. thuringiensis cry2a toxins.	during evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. however, no well-developed in vitro method has been established to create single-crossover libraries. here we present an in vitro template-change polymerase change reaction ...	2016	27097519
a novel xenomonitoring technique using mosquito excreta/feces for the detection of filarial parasites and malaria.	given the continued successes of the world's lymphatic filariasis (lf) elimination programs and the growing successes of many malaria elimination efforts, the necessity of low cost tools and methodologies applicable to long-term disease surveillance is greater than ever before. as many countries reach the end of their lf mass drug administration programs and a growing number of countries realize unprecedented successes in their malaria intervention efforts, the need for practical molecular xenom ...	2016	27096156
inner workings: a big role for a microbial parasite.		0	27095842
testing the causality between cyp9m10 and pyrethroid resistance using the talen and crispr/cas9 technologies.	recently-emerging genome editing technologies have enabled targeted gene knockout experiments even in non-model insect species. for studies on insecticide resistance, genome editing technologies offer some advantages over the conventional reverse genetic technique, rna interference, for testing causal relationships between genes of detoxifying enzymes and resistance phenotypes. there were relatively abundant evidences indicating that the overexpression of a cytochrome p450 gene cyp9m10 confers s ...	2016	27095599
comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue-endemic and non-endemic areas of rajasthan, india.	dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. to investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely stegomyia aegypti (= aedes aegypti), fredwardsius vittatus (= aedes vittatus) and stegomyia albopicta (= aedes al ...	2016	27094337
the global spread of zika virus: is public and media concern justified in regions currently unaffected?	zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil.	2016	27093860
lethal dengue virus infection: a forensic overview.	dengue virus is a single-stranded rna virus that is a member of the family flaviviridae, genus flavivirus. it is usually transmitted by the female aedes aegypti mosquito. dengue fever is a febrile illness caused by 1 of 4 serotypes of the virus, which may progress to dengue hemorrhagic fever or dengue shock syndrome. the mortality rate of untreated dengue shock syndrome is more than 20%. the reported incidence has increased 30-fold for the past 50 years with an estimated 50 to 100 million dengue ...	2016	27093563
characterization of lethal zika virus infection in ag129 mice.	mosquito-borne zika virus (zikv) typically causes a mild and self-limiting illness known as zika fever, which often is accompanied by maculopapular rash, headache, and myalgia. during the current outbreak in south america, zikv infection during pregnancy has been hypothesized to cause microcephaly and other diseases. the detection of zikv in fetal brain tissue supports this hypothesis. because human infections with zikv historically have remained sporadic and, until recently, have been limited t ...	2016	27093158
wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small rna pathways.	the intracellular endosymbiotic bacterium wolbachia can protect insects against viral infection, and is being introduced into mosquito populations in the wild to block the transmission of arboviruses that infect humans and are a major public health concern. to investigate the mechanisms underlying this antiviral protection, we have developed a new model system combining wolbachia-infected drosophila melanogaster cell culture with the model mosquito-borne semliki forest virus (sfv; togaviridae, a ...	2016	27089431
daily rhythms in mosquitoes and their consequences for malaria transmission.	the 24-h day involves cycles in environmental factors that impact organismal fitness. this is thought to select for organisms to regulate their temporal biology accordingly, through circadian and diel rhythms. in addition to rhythms in abiotic factors (such as light and temperature), biotic factors, including ecological interactions, also follow daily cycles. how daily rhythms shape, and are shaped by, interactions between organisms is poorly understood. here, we review an emerging area, namely ...	2016	27089370
detection of autochthonous zika virus transmission in sincelejo, colombia.		0	27089253
an operational framework for insecticide resistance management planning.	arthropod vectors transmit organisms that cause many emerging and reemerging diseases, and their control is reliant mainly on the use of chemical insecticides. only a few classes of insecticides are available for public health use, and the increased spread of insecticide resistance is a major threat to sustainable disease control. the primary strategy for mitigating the detrimental effects of insecticide resistance is the development of an insecticide resistance management plan. however, few exa ...	0	27089119
clinical, virologic, and epidemiologic characteristics of dengue outbreak, dar es salaam, tanzania, 2014.	we investigated a dengue outbreak in dar es salaam, tanzania, in 2014, that was caused by dengue virus (denv) serotype 2. denv infection was present in 101 (20.9%) of 483 patients. patient age and location of residence were associated with infection. seven (4.0%) of 176 patients were co-infected with malaria and denv.	0	27088845
molecular characterization of chikungunya virus, philippines, 2011-2013.	during 2011-2013, a nationwide outbreak of chikungunya virus infection occurred in the philippines. the asian genotype was identified as the predominant genotype; sporadic cases of the east/central/south african genotype were detected in mindanao. further monitoring is needed to define the transmission pattern of this virus in the philippines.	0	27088593
asian genotype zika virus detected in traveler returning to mexico from colombia, october 2015.		0	27088574
projecting month of birth for at-risk infants after zika virus disease outbreaks.	the marked increase in infants born with microcephaly in brazil after a 2015 outbreak of zika virus (zika virus) disease suggests an association between maternal zika virus infection and congenital microcephaly. to project the timing of delivery of infants born to mothers infected during early pregnancy in 1 city in bahia state, brazil, we incorporated data on reported zika virus disease cases and microcephaly cases into a graphical schematic of weekly birth cohorts. we projected that these birt ...	0	27088494
tropical islands as new hubs for emerging arboviruses.		0	27088243
evolutionary biology and genetic techniques for insect control.	the requirement to develop new techniques for insect control that minimize negative environmental impacts has never been more pressing. here we discuss population suppression and population replacement technologies. these include sterile insect technique, genetic elimination methods such as the release of insects carrying a dominant lethal (ridl), and gene driving mechanisms offered by intracellular bacteria and homing endonucleases. we also review the potential of newer or underutilized methods ...	2015	27087849
analysis of significant factors for dengue fever incidence prediction.	many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the k-h model, support vector machines (svm), and artificial neural networks (ann). the time series analysis methodology, particularly arima and sarima, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. the main drawback of these methods is that they do not consider other v ...	2016	27083696
membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction.	understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ...	2016	27083158
first chikungunya outbreak in suriname; clinical and epidemiological features.	in june 2014, suriname faced the first chikungunya outbreak. since international reports mostly focus on hospitalized patients, the least affected group, a study was conducted to describe clinical characteristics of mainly outpatients including children. in addition, the cumulative incidence of this first epidemic was investigated.	2016	27082985
microcephaly and zika virus: neonatal neuroradiological aspects.	the aim of this study is to describe some radiological features in the newborns with microcephaly caused by zika virus infection during pregnancy.	2016	27080092
multi-stakeholder decision aid for improved prioritization of the public health impact of climate sensitive infectious diseases.	the effects of climate change on infectious diseases are an important global health concern and necessitate decisions for allocation of resources. economic tools have been used previously; however, how prioritization results might differ when done using broader considerations identified by local stakeholders has yet to be assessed. a multicriteria decision analysis (mcda) approach was used to assess multi-stakeholder expressed concerns around disease prioritization via focus groups held in quebe ...	2016	27077875
profile distribution of juvenile aedes species in an urban area of malaysia.	dengue fever (df) is an urban vector-borne disease transmitted by aedes aegypti and aedes albopictus. both species deposit their eggs in favorable breeding sites either in natural or artificial containers. an understanding of their habitat characteristics is crucial in curbing df outbreaks	2016	27076510
search and one will find: zika virus everywhere.		2016	27076507
malaria vector anopheles culicifacies sibling species differentiation using egg morphometry and morphology.	the malaria vector anopheles culicifacies (sensu lato) is an important malaria vector in southeast asia which comprises of five sibling species namely a, b, c, d and e. however, only a few forms have been identified as malaria vectors in various endemic countries. currently, for the first time egg morphometry and morphology has been used to differentiate the three known vector sibling species of anopheles culicifacies collected from malaria endemic madhya pradesh state of central india.	2016	27075571
one-step synthesis of polydispersed silver nanocrystals using malva sylvestris: an eco-friendly mosquito larvicide with negligible impact on non-target aquatic organisms.	the synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience with a growing number of biomedical implications. in this study, we investigated the synthesis of polydisperse and stable silver nanoparticles (agnp) using a cheap leaf extract of malva sylvestris (malvaceae). bio-reduced agnp were characterized by uv-visible spectrophotometry, fourier transform infrared spectroscopy (ftir), x-ray diffraction analysis (xrd), atomic force microscopy (afm), scanning electron microscopy ...	2016	27075309
clinical features and neuroimaging (ct and mri) findings in presumed zika virus related congenital infection and microcephaly: retrospective case series study.	to report radiological findings observed in computed tomography (ct) and magnetic resonance imaging (mri) scans of the first cases of congenital infection and microcephaly presumably associated with the zika virus in the current brazilian epidemic.	2016	27075009
a novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.	many vectors of malaria and other infections spend most of their adult life within human homes, the environment where they bloodfeed and rest, and where control has been most successful. yet, knowledge of peri-domestic mosquito behaviour is limited, particularly how mosquitoes find and attack human hosts or how insecticides impact on behaviour. this is partly because technology for tracking mosquitoes in their natural habitats, traditional dwellings in disease-endemic countries, has never been a ...	2016	27075002