Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| host protective immunity to trichinella spiralis in mice: activation of th cell subsets and lymphokine secretion in mice expressing different response phenotypes. | host protective immunity to the intestinal dwelling nematode trichinella spiralis is mediated by cd4+ mesenteric lymph node (mln) cells during the course of intestinal infection. the present study has examined the cytokine production by t cells within the mln of two h-2-compatible strains of mice infected with t. spiralis which differ in the speed at which they expel the parasite from the gut. for both strains of mice, in vitro stimulation of mln cells with a protective worm antigen preparation ... | 1991 | 1836201 |
| characterization of the thoracic duct t-helper cells that co-mediate, with antibody, the rapid expulsion of trichinella spiralis in adult rats. | thoracic duct cells that act synergistically with immune serum or antibody to transfer rapid expulsion of a challenge infection with trichinella spiralis muscle larvae were characterized as ox38+, ox8-, ox22- t helper cells. protective capacity was confined to the dividing t helpers that appeared on days 3-5 in the thoracic duct of rats during a t. spiralis infection. to realize their intestinal priming potential in recipient rats. mhc-compatibility between donor and recipient rat was required. ... | 1991 | 1828879 |
| testing for food-borne parasites, their metabolic products and symbionts. | microscopic animals associated with foods include free-living and saprophytic invertebrates, parasites of hosts other than humans, and parasitic animals specifically designated as food-borne that can infect a human host by the gastrointestinal route. the first general method used to screen for food-borne species was digestion with pepsin and hydrochloric acid at 36 degrees c, based on the "artificial stomach juice" technique for recovering larvae of the nematode trichinella spiralis from muscle. ... | 1991 | 1822919 |
| detection of trichinella by various methods in yugoslavia. | from studies performed on experimentally infected pigs, it was possible to conclude that enzymatic digestion methods are superior to trichinoscopy for inspection of pork for the presence of trichinella spiralis. the analyses included comparative examination of samples taken from five different parts of diaphragm tissue. | 1991 | 1822917 |
| the present status of trichinellosis in thailand. | the epidemiology of trichinellosis in northern thailand may be unique. since 1962, outbreaks of the disease have occurred almost every year. the department of communicable disease control, ministry of public health reported 118 discrete outbreaks of the disease, involving 5,400 patients, 95 of whom have died. epidemiological investigations reveal that the outbreaks have occurred mostly in rural areas, associated with villagers celebrating local and traditional festivals such as the northern thai ... | 1991 | 1822914 |
| epidemiology of swine trichinellosis in yugoslavia. | intensive epidemiological and seroepidemiological studies of trichinella spiralis infection in swine have been carried out in the borough of kladovo, one of the four regions in yugoslavia where trichinellosis is endemic. a high prevalence (4.7%) of trichinellosis was discovered in three neighboring parishes adjoining the bank of the river danube. both geographically and epidemiologically these three parishes constitute a very unique condition that is very vulnerable to t. spiralis infection. stu ... | 1991 | 1822912 |
| epidemiological survey of trichinellosis in yugoslavia. | in a long term epidemiological survey that included different hosts for trichinella spiralis among wild and domestic animals, the parasitological and serological screening revealed a high number of positive animals. the incidence of t. spiralis in grazing pigs was high, 21.76% (170 examined) but most of positive animals were lightly infected (0.016-0.02 l/g). while out of 2,394 pigs raised in small private farms, 1.67% were positive with high infection rates, none of the pigs raised on a modern ... | 1991 | 1822911 |
| present status of trichinellosis in japan. | there have been three mass outbreaks of human trichinellosis in japan. the first was in 1974 in iwasaki, aomori prefecture, involving a group of hunters who ate raw meat of a black bear they had shot. of the 20 people who ate the bear meat raw, 15 showed clinical symptoms of trichinellosis. the second outbreak was experienced in sapporo, hokkaido, in 1980. the patients had eaten the raw meat of a brown bear served in a local restaurant. twelve people were diagnosed positive on the basis of clini ... | 1991 | 1822910 |
| general introduction and epidemiology of trichinellosis. | all studies on trichinellosis have been based on the assumption that there is only one cosmopolitan agent of human and animal trichinellosis; trichinella spiralis (owen, 1835). in the past, swine and only a few synanthropic animals were considered reservoirs, and the presence of the parasite in sylvatic animals was considered rare. breeding experiments before, and then biochemical approaches (isoenzymes and dna analysis), showed the presence of a high genetic variability inside this genus, sugge ... | 1991 | 1822909 |
| [evaluation of 2 serological techniques in the diagnosis of neurocysticercosis: complement fixation reaction and western blot]. | evaluation of two serological techniques for diagnosis cysticercosis: complement fixation reaction and western blot. comparative study that includes 49 sera samples and 30 csf samples belonging to 35 patients with clinical diagnosis of cysticercosis of the cns. as a control group we used 10 sera samples of patients with trichinella spiralis infection, 33 sera samples of patients with hydatid cyst disease, 9 sera samples from patients with oxyuriasis, 52 sera samples of healthy blood donors and 2 ... | 1991 | 1822705 |
| [the composition of the peripheral blood in white mice infected with trichinella spiralis and trichinella pseudospiralis]. | the study of the peripheral blood response in mice with an experimental t. spiralis infection from the 1 to the 60 days of the latter showed leucocytosis, lymphomonocytosis and neutropenia maximal at the 21--35 days of the infection along with hyperthrombocytosis and esr elevation, with the subsequent declination up to the end of the supervision. in experimental p. pseudospiralis infection lymphomonocytosis and esr levels were comparatively lower but eosinophilia was significantly higher. | 1991 | 1818247 |
| [the resistance of muscle larvae of trichinella spiralis to exposure to low temperatures]. | it has been shown that the resistance of muscular larvae of trichinella spiralis to the effect of low temperatures depends on the conditions of freezing and composition of media possessing cryoprotective properties. it has been established that under optimal conditions (medium of microenvironment, conditions of freezing) during the storage in liquid nitrogen muscular larvae of t. spiralis preserve 50 to 60% of their resistance by the character of mobility and 11 to 15% by their capacity for repr ... | 1991 | 1813846 |
| the effects of maternal ethanol consumption on the distribution of leukocyte subsets in the lactating mammary gland of rats. | significant immunological protection is provided to the newborn by the transfer of maternal leukocytes during nursing. the objective of this study was to determine if ethanol ingestion altered the distribution of t, b and accessory cells in the mammary glands of normal rats or in rats infected with trichinella spiralis (t. spiralis). in addition, female rats were fed either lieber/decarli regular (18% protein) or higher protein (25% protein) ethanol-containing liquid diets for pregnant or lactat ... | 1991 | 1804141 |
| study of immune defence against trichinella spiralis in bcg treated mice. | in vivo and in vitro studies were performed to determine the effect of bcg- activated macrophages on 2-hour-old newborn larvae (nbl2) of trichinella spiralis. in vitro studies were done by the technique of antibody-dependent cellular cytotoxicity (adcc) using peritoneal cells of balb/c mice which had been treated with bcg 4-6 days before. no significant differences were found between the percentage of mortality caused by cells from treated animals and that corresponding to the untreated ones (co ... | 1991 | 1799166 |
| [the fatty acids of trichinellae]. | the composition of fatty acids of lipids in the muscles of rats and larvae of trichinella spiralis and t. nativa developing in them were studied. both species are characterized by practically the same composition of fatty acids, only in the frost-resistant species t. nativa there was a sufficient amount (up to 3.5%) of docosapenta- and docosahexaenic acids (22:5 and 22:6). the comparison of the content of individual fatty acids in larvae and in muscles of the host by means of statistical correla ... | 1991 | 1792101 |
| [effects of immunoactivity on ascaris suum infection in mice]. | the immune response to sheep red blood cell (srbc) was monitored in the mice infected with ascaris suum or trichinella spiralis. the effects of the infection with t. spiralis or the injection with cyclophosphamide(cy) as an immunosuppression agent prior to challenge infection with the embryonated eggs of a. suum were monitored in mice by means of the level of infection with a. suum and cellular and humoral immune response to srbc. following the oral administration of 1,000 eggs of a. suum to mic ... | 1991 | 1786254 |
| investigations with enzyme-linked immunosorbent assays for trichinella spiralis and toxoplasma gondii in the dutch 'integrated quality control for finishing pigs' research project. | in the framework of the dutch field trial 'integrated quality control (iqc) for finishing pigs' elisa (screening) techniques were used to detect animals seropositive for toxoplasma gondii and/or trichinella spiralis. the aim was to determine whether farms which consistently delivered seropositive pigs could be detected and monitored (defined as 'problem farms'). the investigation involved 120 farms and three slaughterhouses, and a total of 23,348 serum samples were examined. in addition, all pig ... | 1991 | 1776233 |
| the relationship between intestinal location and fecundity in adult trichinella spiralis. | adult female worms recovered from the jejunum of rats infected per os with 1000 larvae of trichinella spiralis were significantly more fecund (peak jejunal fecundity = 35.7 +/- 3.1 newborn larvae per female) than females recovered from the terminal ileum (9.3 +/- 4.1 larvae per female) in the same infections. the majority of the adult worms were established in those sections of the small intestines that produced the most fecund females (r = 0.92; p less than 0.05). worm fecundity is believed to ... | 1991 | 1774121 |
| the role of the antibody fc region in rapid expulsion of trichinella spiralis in suckling rats. | when an igg2c monoclonal antibody specific for trichinella spiralis muscle stage larvae was cleaved with pepsin to yield f(ab')2 fragments, the latter retained their capacity to cause mucus entrapment and rapid expulsion of larvae from the intestines of suckling rats. when fed to pups, the f(ab')2 fragments of this antibody and the f(ab')2 fragments of a similarly prepared igg2a antibody caused mucus entrapment of muscle larvae (ml), demonstrating that trapping is not dependent upon the fc regio ... | 1991 | 1769702 |
| intestinal mucus entrapment of trichinella spiralis larvae induced by specific antibodies. | entrapment of muscle larvae (ml) occurred in vitro when antibodies specific for trichinella spiralis were added directly to intestinal mucus from normal non-immunized rats or when mucus was collected from pups suckling a t. spiralis-infected dam. normal rat serum immunoglobulins failed to promote mucus entrapment and complement did not appear to play a part in the entrapment process. differences were not observed in the efficiency of entrapment of ml by mucus harvested from different regions of ... | 1991 | 1769701 |
| experimental trichinella spiralis infection in sheep. | during susceptibility studies of non-specific hosts, three merino sheep were infected with 3000, 5000 or 7000 trichinella spiralis larvae by gavage. clinical, physiological and serological parameters were assessed during the experiment. on the 152nd day p.i., animals were necropsied and, using artificial digestion methods, numbers of trichinella larvae in muscle tissues were determined. the most infected parts were masseters with 3122 larvae g-1 muscle, 5526 larvae g-1 muscle and 4058 larvae g-1 ... | 1991 | 1763480 |
| identification of sylvatic trichinella (t3) in foxes from france. | thirty-three foxes (vulpes vulpes) from a sample of 1912 collected in france were found to be infected with trichinella spp. four isolates were obtained for genetic identification. isoenzymatic and biological analysis of these isolates revealed the presence of two distinct genetic types of trichinella, trichinella spiralis s.str. (t1) and trichinella sp. (t3) (trichinella nelsoni according to soviet authors) in the fox population. the reproductive capacity index of these isolates in wistar rats ... | 1991 | 1763479 |
| a repetitive dna probe specific for a north american sylvatic genotype of trichinella. | a partial genomic dna library constructed in puc 13 using dna from a sylvatic isolate of trichinella spiralis (t. spiralis t5) was differentially screened with radiolabeled homologous genomic dna and with dna from t. spiralis t1. one clone was identified and designated pupb-3.7 which, by slot blot and southern blot analyses, reacted specifically with t. spiralis t5 dna and did not cross-react with dna from any other t. spiralis genotype. the 482-bp repetitive sequence which is 70% rich in a and ... | 1991 | 1762626 |
| [the effect of toluene and ammonia on the invasive process and immunological indices in experimental trichinelliasis in mice]. | rap mice were exposed to toluene (t) inhalation for 10 days before invasion with 20 trichinella spiralis larvae per g body weight (moderate infection). this resulted in diminished number of intestinal parasites in the presence of greater number of mast cells in the peritoneal exudate, higher ige production, enhanced cell adhesion to trichinella larvae and of migration of splenic lymphocytes. simultaneous inhalation of t and ammonia diminished the immune stimulating effect of the former. the numb ... | 1991 | 1758365 |
| stimulated chemotactic response in neutrophils from trichinella pseudospiralis-infected mice and the neutrophilotactic potential of trichinella extracts. | during infection with trichinella pseudospiralis a strong neutrophil response is evident in the peripheral circulation of the mouse. this study compared the chemotactic response of neutrophils from uninfected, t. pseudospiralis-infected and trichinella spiralis-infected mice to extracts from adult worms, newborn larvae and muscle-stage larvae of both species of parasite. the chemotactic response of neutrophils from t. pseudospiralis-infected mice to zymosan-activated mouse serum (zams) was signi ... | 1991 | 1757190 |
| helminth parasites of the raccoon (procyon lotor) from north-central arkansas. | twenty-three species of helminths (4 trematodes, 2 cestodes, 14 nematodes, and 3 acanthocephalans) were found in the gastrointestinal tract, lungs, pancreas, tongue, urinary bladder, and subcutaneous tissues of 30 live-trapped or hunter-shot raccoons from north-central arkansas between november 1989 and april 1990. helminths were not detected in the brain, diaphragm, gallbladder, heart, liver, reproductive system, or trachea. each raccoon examined was infected with 3 or more of the following hel ... | 1992 | 1738063 |
| immunity to trichinella spiralis infection in vitamin a-deficient mice. | vitamin a-deficient (a-) mice make strikingly poor igg responses when they are immunized with purified protein antigens. previously, we showed that a- t cells overproduce interferon gamma (ifn-gamma), which then could inhibit interleukin 4 (il-4)-stimulated b cell igg responses. to determine if the altered ifn-gamma regulation pattern and its immunological consequences would extend to a natural infection, we studied mice infected with the parasitic helminth trichinella spiralis. the course of th ... | 1992 | 1730911 |
| diagnostic tests for trichinella spiralis infection in pigs. a comparative study of elisa for specific antibody and histamine release from blood cells in experimental infections. | a study on the histamine release test (hr) for the demonstration of infections with trichinella spiralis in pigs was carried out on 18 pigs, six infected with 200 larvae, six infected with 5000 larvae and six non-infected (control group). the results obtained by hr during a 7 week infection were compared with those of the enzyme-linked immunosorbent assay (elisa). all inoculated pigs were found to be positive on day 40 post-inoculation (p.i.) by necropsy examination of selected muscle groups, wi ... | 1991 | 1720267 |
| trichinella spiralis: antigenic epitopes from the stichocytes detected in the hypertrophic nuclei and cytoplasm of the parasitized muscle fibre (nurse cell) of the host. | monoclonal antibodies raised against antigens present in the excretions/secretions (e/s) of larval trichinella spiralis, polyclonal antibodies raised against e/s and antisera from rabbits and pigs infected with t. spiralis were used in conjunction with immunocytochemical techniques to detect antigens in sections of muscle from mice that had been infected with t. spiralis for 15 or 30 days. the antibodies recognized epitopes in the stichocytes, on the surface of the cuticle, in the lumen of the o ... | 1991 | 1710049 |
| trichinella spiralis: influence of an immunodominant, carbohydrate-associated determinant on the host antibody response repertoire. | host antibody responses to the g2.1 epitope, a carbohydrate-associated determinant shared by several trichinella spiralis glycoproteins, were examined by competitive inhibition enzyme-linked immunosorbent assay (elisa). the g2.1 epitope dominated the akr/j mouse antibody response whether the antigens were injected or introduced through infection, as determined by the serum blocking ability of a g2.1 epitope-specific monoclonal antibody (mab). serum t. spiralis-binding activity from several other ... | 1991 | 1709113 |
| inhibition of anaphylaxis-evoked intestinal fluid secretion by the dual application of an h1 antagonist and cyclooxygenase inhibitor. | the regulation of anaphylaxis-mediated fluid secretion by the small intestine was examined in rats immunized by infection with trichinella spiralis and reinfected by intraduodenal injection with l1 larvae. net fluid secretion, which was measured as the volume of fluid present in the intestine 30 minutes after the challenge infection, was significantly greater in both actively and passively immunized rats than in nonimmune rats. the amount of fluid recovered from the immune host was equivalent to ... | 1991 | 1705907 |
| neuroimmune regulation of colonic secretion in guinea pigs. | the role of submucosal neurons in anaphylactic-like responses in colonic epithelium from immunized guinea pigs was examined 6-8 wk after inoculation with 2 x 10(3) infective trichinella spiralis larvae. serosal addition of t. spiralis antigen (20 micrograms/ml) to muscle-stripped segments of colon set up in flux chambers evoked a maximum increase in short-circuit current within 5 min in immune, but not nonimmune, guinea pigs. quercetin, a membrane-stabilizing drug, and pyrilamine, a histamine h1 ... | 1991 | 1705101 |
| antigenic differences between trichinella spiralis and t. pseudospiralis detected by monoclonal antibodies. | antigenic differences between trichinella spiralis and t. pseudospiralis were established using two monoclonal antibodies (mabs) that show different specificities to muscle larvae of the two variants. enzyme-linked immunosorbent assay (elisa) revealed that mab 3g6 reacts positively against t. spiralis, t. nelsoni, t. nativa and t. pseudospiralis, whereas mab 3e10 does not react with t. pseudospiralis under the same experimental conditions. these antigenic differences were confirmed after preabso ... | 1991 | 1704628 |
| [mechanisms of t-cell-mediated hypersensitivity in mice infected with the intestinal helminth trichinella spiralis]. | intestinal hypersensitivity reactions during parasitic helminth infections are t-cell-dependent. little is known about the characteristics of t-cells that initiate and regulate intestinal inflammation or about how they do this. in this paper data are presented that indicate that during trichinella spiralis infections in mice t-cells regulate intestinal inflammation in the same way that they regulate delayed-type hypersensitivity reactions in the skin and cellular immune responses against tumors. | 1990 | 1701925 |
| characterization of trichinella spiralis antigens sharing an immunodominant, carbohydrate-associated determinant distinct from phosphorylcholine. | the biochemical and immunochemical characteristics of t. spiralis molecules (group ii antigens) sharing an immunodominant epitope were examined. six major proteins, ranging from 43-68 kda, and from pi 5.0-6.3, express the determinant. together, they account for at least 3% by weight of the total protein in l1 larval homogenate. the antigens are glycosylated. following periodate oxidation, they reacted with biotin aminocaproyl hydrazide, and treatment with trifluoromethanesulfonic acid decreased ... | 1990 | 1697936 |
| molecular analysis of the gene encoding an antigenic polypeptide of trichinella spiralis infective larvae. | the gene encoding an antigenic polypeptide of trichinella spiralis infective larvae was studied using recombinant dna techniques. cdna synthesized from poly(a)-rich mrna from t. spiralis infective larvae was ligated into phage vector lambda gt11 dna and packaged in vitro. the phages were propagated on escherichia coli and a lambda gt11 expression library was constructed. a cdna clone encoding a 46 kda antigenic polypeptide was selected by immunoscreening of the library and identified by the epit ... | 1990 | 1692576 |
| the acute-phase protein response in parasite infection. nippostrongylus brasiliensis and trichinella spiralis in the rat. | during acute inflammation, the mammalian liver responds with increased production and secretion of a series of plasma glycoproteins, collectively termed the acute-phase proteins, resulting from the release at the site of inflammation of polypeptide cytokines, including il-1 and il-6, which interact with receptors on hepatocytes and alter gene expression. this attribute of the systemic acute-phase response was studied throughout the course of infection with two nematode parasites in rats. signifi ... | 1990 | 1692304 |
| the mouse antibody response to trichinella spiralis defines a single, immunodominant epitope shared by multiple antigens. | immunoblot analysis was used to characterize the trichinella spiralis l1 larval ag recognized by antisera from t. spiralis-infected akr/j mice. antisera were analyzed for reactivity with crude worm extract, excretory/secretory proteins and cuticle proteins from l1 larvae. the response was biphasic; antibodies against one set of ag were detected 13 days after infection (group i ag), and antibodies against a different set of ag were detected 35 days after infection (group ii ag). excretory/secreto ... | 1990 | 1691229 |
| direct evidence that the cuticle surface of trichinella spiralis muscle larvae shares antigenicity with stichocyte alpha-granules and the esophagus-occupying substance. | antibodies against the cuticle surface of trichinella spiralis muscle larvae were purified by means of immunoaffinity chromatography and incubated with ultrathin sections of muscle larvae. major constituents of the parasite reactive with the purified antibodies included the cuticle surface, stichocyte alpha-granules, and the esophagus occupying substance of the muscle larvae. thus the present data suggest that the cuticle surface is an antigenically different entity from the cuticle inner layers ... | 1990 | 1690799 |
| antigen-specific lymphocyte proliferative responses in inbred mice after trichinella spiralis infection. | the in vitro antigen-specific lymphoproliferative response of spleen, mesenteric lymph node (mln), and coeliac lymph node (cln) cells taken from various strains of inbred mice infected with trichinella spiralis was assessed. in most experiments cell populations were stimulated with excretory/secretory antigens (esa) derived from adult and larval worms. lymphoid cells collected 5-7 days postinfection were usually the most responsive to esa as measured by [3h]thymidine uptake. spleen cells were mo ... | 1990 | 1688939 |
| ifn-gamma- and il-5-producing cells compartmentalize to different lymphoid organs in trichinella spiralis-infected mice. | the differential induction of cytokines associated with th1 and th2 subsets has recently been described during trichinella spiralis infection. increased levels of resistance appear to correlate with elevated levels of the th1-associated cytokines, ifn-gamma and il-2. in the present report, a filter immunoplaque assay is used to quantify the actual numbers of cells that secrete ifn-gamma and il-5. it is demonstrated that, in t. spiralis-infected b10.q mice, th1- and th2-associated responses are c ... | 1991 | 1675654 |
| characterization of a noncyst-forming isolate of trichinella from a wild boar in yugoslavia. | an isolate of trichinella obtained from a wild boar in yugoslavia did not form cysts in the musculature of its natural host. subsequent inoculation into experimental hosts demonstrated that some larvae became encysted only after extended time periods, whereas others remained unencapsulated. histological staining of larvae in the musculature demonstrated no deposition of collagen typically seen for trichinella spiralis spiralis, trichinella spiralis nativa, or trichinella spiralis nelsoni. the yu ... | 1991 | 1672709 |
| trichinella pseudospiralis overcomes innate resistance of the chinese hamster to trichinella spiralis. | infectivity of trichinella pseudospiralis in chinese hamsters was greater than that of trichinella spiralis. concurrent infection with these two species of parasite was accompanied by an increase in infectivity of t. spiralis. the infectivity of t. pseudospiralis, but not that of t. spiralis, was significantly influenced by host age. regardless of host age, t. pseudospiralis was more infective for the chinese hamster than was t. spiralis. early during infection, enteritis (myeloperoxidase activi ... | 1991 | 1664077 |
| t-lymphocyte modulation of intestinal muscle function in the trichinella-infected rat. | jejunal longitudinal muscle from trichinella-infected sprague-dawley rats generates increased tension in vitro 6 days after infection. to investigate the extent to which this increase is dependent on t lymphocytes, smooth muscle contraction was examined in athymic rats (rnu/rnu) and in their euthymic (rnu/+) littermates 6 days after infection. in both thymus-bearing and athymic rats, the highest concentrations of trichinella were found in the jejunum where mucosal myeloperoxidase activity was in ... | 1991 | 1646141 |
| trichinella spiralis: dose dependence and kinetics of the mucosal immune response in mice. | the role of the mucosal immune response in helminth infections is not clear. in this study, the dose dependence and kinetics of the mucosal immune response to trichinella spiralis were determined in experimentally infected swiss webster and balb/c mice. the primary mucosal isotype was siga, although igg was also detected, and primary infections with 10 and 150 larvae produced an anamnestic response on challenge. the mucosal and systemic immunoglobulin responses were dose dependent in both primar ... | 1992 | 1639167 |
| trichinella spiralis: modifications of the cuticle of the newborn larva during passage through the lung. | a scintigraphic method was developed to study the distribution of radioactivity after iv injection of 131i-labeled trichinella spiralis newborn larvae into normal rats. it was found that the radioactivity was immediately retained in the lungs and thereafter slowly released, with a mean transit time in excess of 9 hr, as calculated by image analysis. at various times after iv injection of newborn larvae into normal mice, the lungs were removed and parasites were recovered and counted. fifty to se ... | 1992 | 1639155 |
| taxonomic revision of the genus trichinella. | the analysis of genetic, biochemical, and biological data on about 300 trichinella isolates, reported in the literature, allows a taxonomic revision of this genus. we propose the recognition of 5 sibling species, trichinella spiralis (owen, 1835) sensu stricto; trichinella nativa britov and boev, 1972; trichinella pseudospiralis garkavi, 1972; trichinella nelsoni britov and boev, 1972 sensu stricto; and trichinella britovi n. sp., on the basis of biochemical and biological characteristics. trich ... | 1992 | 1635024 |
| biological characterization of trichinella isolates from various host species and geographical regions. | forty isolates of trichinella collected from 5 continents were compared for 7 biological characters: newborn larvae produced per female worm cultured in vitro at the seventh, eighth, and ninth day postinfection, host muscle nurse cell development time, reproductive capacity index in rats and chickens, and resistance of muscle larvae to freezing. the isolates also were compared by analyses of an environmental character of the location from which they were isolated: the isotherms for january and j ... | 1992 | 1635023 |
| allozyme analysis of trichinella isolates from various host species and geographical regions. | allozyme analysis was carried out on 152 trichinella isolates from synanthropic and wild animals and from humans; the isolates were collected from 5 continents. the analysis, involving 27 enzymes, revealed the presence of 8 distinct gene pools, termed t1-t8. four of the genetic groups represent the 4 previously proposed species: trichinella spiralis sensu stricto (t1), trichinella nativa (t2), trichinella nelsoni (t7), and trichinella pseudospiralis (t4). the other 4, t3, t5, t6, and t8 are dist ... | 1992 | 1635022 |
| host resistance to trichinella spiralis infection in rats and mice: species-dependent effects of cyclophosphamide exposure. | host resistance to trichinella spiralis infection was compared in male rats (f344) and female mice (c57bl/6j) following various cyclophosphamide (cy) treatment schedules. doses of cy given to mice were adjusted by body surface area to be comparable to rat doses. adult parasite elimination was not affected by oral administration of 1.5, 3 or 6 mg cy/kg per day to rats or 1.05, 2.1 or 4.2 mg cy/kg per day to mice for 10 days. in rats, resistance was suppressed by a single oral dose of 80 mg/kg giv ... | 1992 | 1631907 |
| trichinosis with severe myopathic involvement mimicking polymyositis. report of a family outbreak. | we describe an outbreak of trichinosis in 3 members of a rural family. in the 3 patients eating raw pork was the source of infection. they presented with myalgias and severe proximal muscle weakness mimicking polymyositis. the diagnosis was made by demonstration of larvae of trichinella spiralis in the muscle biopsy and also by the presence of anti-trichinella antibodies detected by double immunodiffusion in their sera. we call attention to the unusual clinical presentation of trichinosis in our ... | 1992 | 1629835 |
| inflammation causes hyperplasia and hypertrophy in smooth muscle of rat small intestine. | inflammation of the rat jejunum with trichinella spiralis causes altered smooth muscle contractility by day 6 postinfection (pi). we investigated the association of structural change in the smooth muscle layers with inflammation. by day 6 pi, smooth muscle area in cross sections of jejunum increased (p less than 0.05) in longitudinal (lm) and circular (cm) muscle layers. nuclei counting in cross sections showed that cell number increased two- to threefold in cm and lm, and this increase was not ... | 1992 | 1616034 |
| characterization of cellular and molecular immune effectors against trichinella spiralis newborn larvae in vivo. | the cellular and molecular immune effectors that participated in host immunity against trichinella spiralis newborn larvae were characterized in vivo using ao rats. donor rats were immunized with 2,000 muscle larvae orally or 11,400 newborn larvae i.v. immune serum and cells from spleen, peripheral lymph nodes, mesenteric lymph node, thoracic duct lymph and the peritoneal cavity were obtained from donor rats 10-21 days after infection and transferred into normal recipient rats. the control recip ... | 1992 | 1611662 |
| [trichinosis in children. a report of 8 cases]. | a comparative study between 8 cases of trichinosis and 16 of typhoid fever is presented in this paper; all of them were attended in the inp during the period from 1970 to 1990. the diagnosis was established under epidemiologic, clinic and laboratory bases. the range of age of these patients was from 10 to 14 years, from low socioeconomic status; half of the cases had history of in take of infested pork meat. the most characteristic symptoms were: fever, myalgia and limitation of muscular movemen ... | 1992 | 1605875 |
| ultrastructure, antigenicity, and histochemistry of stichocyte granules of adult trichinella spiralis. | the stichosome of adult trichinella spiralis was studied to determine its ultrastructural, antigenic, and histochemical characteristics. stichocytes of adult worms had 2 types of granules, type i and type ii, the ultrastructure of which was different from those of muscle larvae. both types of granules consisted of a membrane surrounding a homogeneous matrix, and type i granules were rounder than type ii granules. sera from c3h mice immunized against excretory-secretory products of muscle larvae ... | 1992 | 1597798 |
| trichinella spiralis: evidence that mice do not express rapid expulsion. | the rapid expulsion of trichinella spiralis by mice of a variety of inbred and f1 mouse strains was examined. mice were reinfected once with t. spiralis during and immediately after the natural termination of a primary infection and worm rejection was measured less than or equal to 24 hr after the challenge. the results showed that the challenge (super)infection was consistently rejected by all mouse strains before rejection of the adult worms from the primary infection commenced. rejection of t ... | 1992 | 1592093 |
| trichinella spiralis: activation of complement by infective larvae, adults, and newborn larvae. | the ability of trichinella spiralis to activate complement (c) has been addressed by several investigators. however, these investigators employed methods in which either detection of c fragments on the parasite surface or the adherence of leukocytes to the parasite was considered an indication of c activation. the present studies were undertaken to examine: (a) whether activation of c occurs via the classical and/or alternative pathway, (b) at which stage(s) of the parasite c activating capacity ... | 1992 | 1582481 |
| immunopathological studies on the encystation phase of experimental trichinosis after cortisone and cyclophosphamide treatment. | efficacy of cortisone and cyclophosphamide on the muscle phase of trichinella spiralis in albino rats, before and after complete encapsulation, was studied by haematoxylin and eosin staining for histopathological changes and by the indirect immunoperoxidase test for the extent of antigen deposition. results showing beneficial effects of cortisone and hazardous action of cyclophosphamide are discussed in details. this study indicates that the use of cortisone in the treatment of trichinosis remai ... | 1992 | 1578166 |
| histochemical studies in experimental trichinosis. | the study of the behaviours of trichinella spiralis in the host tissues from the histochemical point of view will throw a light on parallelism of the destruction process by the infection and the instruction of the host tissues, i.e., the catabolic effect of the parasite as an indicator of the severity of infection and the anabolic response by the host as a criteria of cure. so, this study revealed a definite increase in catabolic enzymes as acid phosphatase activities. a relative disturbance and ... | 1992 | 1578164 |
| an ultrastructural study of s. mansoni adult worms after administration of trichinella spiralis antigen in vivo. | the ultrastructure of s. mansoni adult worms recovered from mice that received t. spiralis antigen prior to s. mansoni infection is described. recorded changes in the tegument of the adult male s. mansoni; complex reticular network of surface pits, complete disruption in some parts, reduction in size of muscle blocks below the tegument, appearance of abundant electron dense lysosomes in the muscle layer. recorded changes in the reproductive organs of the adult female s. mansoni; severe disorgani ... | 1992 | 1578157 |
| the identification and characterization of a break within the large subunit ribosomal rna of trichinella spiralis: comparison of gap sequences within the genus. | a break was identified in the large subunit ribosomal rna of trichinella spiralis that results in its dissociation into 2 smaller fragments of approximately equal length. the approximate location of the break within the encoding gene was mapped from subcloned rdna fragments by s1 protection experiments. the boundaries of the break were determined by cdna primer extension and s1 nuclease protection assays. the excised fragment (gap sequence) was localized to expansion segment 5 within domain iv f ... | 1992 | 1574086 |
| rapid expulsion of trichinella spiralis in adult rats mediated by monoclonal antibodies of distinct igg isotypes. | the role of igg in rapid expulsion of trichinella spiralis in adult rats was analysed. in this experimental model, rats were first infected with an unrelated nematode heligmosomoides polygyrus, then 5-14 days later, immune serum, its fractions, or igg monoclonal antibody (mab) was transferred. rats were challenged with t. spiralis muscle larvae 24 hr after antibody transfer and intestinal worms counted at various times, up to 24 hr, after challenge. provided rats were exposed to h. polygyrus fir ... | 1992 | 1572697 |
| t lymphocyte dependent enteropathy in murine trichinella spiralis infection. | mice infected with trichinella spiralis developed significant enteropathy, comprising villus atrophy, crypt hyperplasia, goblet cell hyperplasia and a decrease in intra-epithelial lymphocyte numbers by 10 days post-infection, when most of the parasites had been expelled from the gut. however, worm expulsion was prevented by treatment with cyclosporin a and, despite a continued parasite burden, cyclosporin a treated animals had no villus atrophy or changes in inflammatory cell numbers. these resu ... | 1992 | 1570173 |
| experimental reorganization of host muscle cells by excretory/secretory products of infective trichinella spiralis larvae. | 1992 | 1566315 | |
| susceptibility of adult heligmosomoides polygyrus to intestinal inflammatory responses induced by heterologous infection. | adult h. polygyrus are capable of surviving for many months after primary exposure of mice to infective larvae, raising the possibility that worms of this species have inherent resistance to intestinal immune responses. accordingly experiments were carried out to determine whether h. polygyrus are resistant to the inflammatory changes elicited during the acute phase of the intestinal response to trichinella spiralis. adult worms were expelled from mice when their presence coincided with the most ... | 1992 | 1563923 |
| identification of diagnostic antigens from trichinella spiralis. | the western blotting technique was used to determine the antigens of trichinella spiralis muscle larvae that were recognized by antibodies in sera from humans and pigs displaying t. spiralis infections. this resulted in the identification of several antigens that were recognized by all sera. some of these antigens, notably those that were recognized during the early stage of infection, cross-reacted with antibodies to other parasites. this cross-reactivity was caused by the presence of phosphory ... | 1992 | 1557322 |
| distribution of mast cells in intestinal muscle of nematode-sensitized rats. | we examined the distribution and functional integrity of mast cells in intestinal longitudinal muscle in rats sensitized by two previous infections with trichinella spiralis. a segment of jejunum was excluded from the gut before infection, and the remainder of the gut was anastomosed. few mast cells were seen in muscle of noninfected control rats except in the region of the jejunal anastomosis. in rats sensitized by t. spiralis infection, mast cells were increased in number in the jejunum and th ... | 1992 | 1550236 |
| [a family outbreak of trichinosis acquired in israel]. | trichinosis is a parasitic disease transmitted to man by ingestion of contaminated raw meat, usually pork, containing cysts of trichinella spiralis. formerly appearing as serious epidemics, it has now become uncommon due to public health control measures. since the jewish and moslem religions forbid consumption of pork, the disease is even rarer in israel. the literature indicates that all the cases of trichinosis hospitalized in israel were contaminated abroad. we describe an israeli family in ... | 1992 | 1526560 |
| il-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of trichinella spiralis-infected mice. | mouse bone marrow-derived mast cells (bmmc) obtained by culturing progenitor cells with ril-3 express mouse mast cell protease (mmcp)-5 mrna but not mmcp-1 mrna or mmcp-4 mrna. in terms of mast cell differentiation, these transcripts encode one early-expressed and two late-expressed chymases, respectively. cdna and crna probes were used in rnase protection assays and rna blot analyses to study the expression of these three homologous protease genes in cultured mast cells and in helminth-infected ... | 1992 | 1517575 |
| prevalence of trichinella spiralis in black bears (ursus americanus) from newfoundland and labrador, canada. | tongue and diaphragm samples from 158 black bears (ursus americanus) from newfoundland and labrador were examined for trichinella spiralis. no larvae were detected in samples from the island of newfoundland but one animal from the labrador samples was infected. the results of this and other studies suggest a lack of involvement of the black bear in a sylvatic cycle of t. spiralis in eastern canada. | 1992 | 1512886 |
| a trichinosis outbreak among southeast asian refugees. | the number of cases of trichinosis reported to centers for disease control has declined steadily, with an average of only 44 cases per year from 1984 through 1988. this decline was almost entirely due to a reduction in cases acquired from ingestion of fresh commercial pork. however, from july 21 through september 3, 1990, 90 (72%) of 125 southeast asian refugees from six states and canada developed trichinosis after attending or eating pork sausage taken from a wedding held in des moines, iowa, ... | 1992 | 1510086 |
| prevalence of antibodies to toxoplasma gondii and trichinella spiralis in 509 pigs from 31 farms in oahu, hawaii. | serum samples from 509 pigs from 31 farms in oahu, hawaii were examined for antibodies to toxoplasma gondii using the agglutination test in dilutions of 1:25, 1:50, and 1:500 and for trichinella spiralis using the enzyme-linked immunosorbent assay. toxoplasma gondii antibodies were found in 48.5% of pigs. antibody titers were: 5.1% positive at 1:25 dilution, 28.6% positive at 1:50 dilution and 14.7% positive at 1:500 dilution. the prevalence of toxoplasma gondii antibodies in garbage-fed pigs (6 ... | 1992 | 1496803 |
| schistosome antigen gp50 is responsible for serological cross-reactivity with trichinella spiralis. | the 50-kda component (gp50) present in schistosoma mansoni eggs and secretions of the various life stages of the parasite was recognized by experimentally infected mice and by humans with s. mansoni, schistosoma haematobium, and schistosoma japonicum infection. all sera reacting with crude s. mansoni-soluble egg antigens (sea) also reacted strongly with gp50 in enzyme-linked immunosorbent assay. no reactivity against gp50 was seen with sera from individuals without schistosomiasis, with the exce ... | 1992 | 1491314 |
| allozymic and biological characters of trichinella pseudospiralis isolates from free-ranging animals. | to evaluate biological and biochemical variability in nonencapsulated trichinella isolates, biological and allozymic studies were conducted on isolates of trichinella collected from a raptoral bird (aquila rapax) and a fox (vulpes corsac) in kazakhstan and from a dasyurid marsupial (dasyurus maculatus) on the island of tasmania, australia. allozyme profiles of bird and marsupial isolates showed close similarity with the type isolate of trichinella pseudospiralis. the avian and fox isolates succe ... | 1992 | 1491304 |
| proteolytic enzymes from trichinella spiralis larvae. | trichinella spiralis larvae infect their hosts by the penetration of small intestine enterocytes. the exact mechanism of penetration is unknown, but the presence of proteolytic enzymes is suspected. in this study, whole worm extracts and excretory-secretory (es) components were obtained and their proteolytic enzymes examined. enzymes from worm extracts were capable of hydrolysing azocoll, a general protease substrate in a wide range of ph (2-8), with maximal activity at ph 5. trichinella spirali ... | 1992 | 1485414 |
| significance of diaphragm sampling for determining larvicidal effect of flubendazole and mebendazole on trichinella spiralis in mice. | 1992 | 1479783 | |
| persistence of igg, igm, and ige antibodies in human trichinosis. | an enzyme-linked immunosorbent assay (elisa) was used to detect serum igg, igm, and ige antibodies against trichinella spirals s. str. following an outbreak of human trichinosis in 1989 in chiang rai province, thailand, for two years and seven months in order to observe diagnostic sensitivity of elisa as well as the persistence of antibodies. it was found that the sensitivity of igg-elisa, as determined from biopsy-positive cases, reached 100% on the 50th day of infection. for igm-elisa, peak se ... | 1992 | 1470835 |
| immunosuppression of in vivo and in vitro lymphocyte responses in swine induced by trichinella spiralis or excretory-secretory antigens of the parasite. | the in vivo and in vitro effects of trichinella spiralis excretory-secretory (es) antigens on porcine peripheral blood lymphocyte (pbl) responses induced with mitogens (phytohemagglutinin, pha; concanavalin a, con a; pokeweed mitogen, pwm) or unrelated antigen (protein a) were studied to determine whether es antigens depress lymphocyte responses in experimental swine trichinosis, and/or if this response was manifested after lymphocytes from infected pigs had been pretreated with es antigens. add ... | 1992 | 1466133 |
| abnormal thymus development and impaired function of the immune system in rats after prenatal exposure to aciclovir. | aciclovir (synonym: acyclovir) causes abnormal thymus development in rats. after treatment on day 10 of gestation a weight reduction of the organ is obvious in 21-day-old fetuses which persists postnatally. adult male rats exposed in utero to one or three injections of 100 mg aciclovir/kg body wt given to the dam on day 10 of pregnancy showed a reduction of the thymus weight to 333 +/- 158 mg and 276 +/- 61 mg (control: 428 +/- 92 mg; n = 10). corresponding alterations were detectable in female ... | 1992 | 1463390 |
| [the helminth fauna of the red fox (vulpes vulpes linne, 1758) in nordhessen and ostwestfalen. 2. nematodes]. | between november 1989 and june 1990 a total number of 397 foxes were examined for the presence of nematodes in the stomach and the small intestine and 403 foxes for the presence of trichinella spiralis larvae. the animals came from the districts of kassel, arnsberg and detmold. in 32.7% of the foxes infections with toxocara canis were found, in 11.1% toxascaris leonina, in 3.8% ancylostoma caninum, in 3.5% uncinaria stenocephala and in 2.3% capillaria species. in most cases the number of nematod ... | 1992 | 1459030 |
| trichinosis from wild boar meat in gojjam, north-west ethiopia. | an outbreak of trichinosis associated with ingestion of meat from a wild boar in gojjam administrative region, ethiopia, is reported. of the 30 soldiers, 20 who ate the meat raw, became ill and 5 of them were admitted to the armed forces general hospital (afgh). even though they presented with a typical history and clinical features the disease was not even suspected at the peripheral hospital. the diagnosis was confirmed, at the afgh, by deltoid muscle biopsy in all the 5 cases. trichinosis bei ... | 1992 | 1455537 |
| isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7. | a gene that encodes mouse mast cell protease (mmcp) 7 (also known as mouse mast cell tryptase 2) was isolated by genomic cloning with a cdna that encodes mmcp-6, a tryptase in serosal mast cells. cdnas encoding mmcp-7 were isolated from a bone-marrow-derived mast cell cdna library. the mmcp-7 gene spans 2.3 kilobases and contains five exons rather than six, as found in the mmcp-6 and human mast cell tryptase i genes. comparison of the 5' end of the transcript with the genomic sequence indicated ... | 1992 | 1454796 |
| distribution of lymphocyte subsets in rat milk from normal and trichinella spiralis-infected rats. | we have shown that t. spiralis-specific t lymphocytes can mediate maternal-to-neonatal immunity during lactation. this study addresses the change of lymphocyte populations in rat milk during normal and disease conditions. two color flow cytometric analysis was performed for milk lymphocytes. t cells (ox19+) made up 45% of rat milk lymphocyte population. t helper cells (th) composed 35% of total t cells while t cytotoxic/suppressor (tcs) cells constituted 34%, giving a th/tcs ratio of 1.03. the c ... | 1992 | 1453391 |
| the effects of maternal ethanol consumption on lactational transfer of immunity to trichinella spiralis in rats. | transient immunity to the intestinal parasite trichinella spiralis can be transferred from the mother to the neonate during lactation. the goal of this study was to determine whether maternal ingestion of ethanol during pregnancy and lactation inhibited expression of anti- t. spiralis immunity in nursing pups. groups of female rats were infected with 1000 t. spiralis l1 larva, mated, and fed either ethanol-containing or isocaloric liquid diets and maintained on diets through pregnancy and lactat ... | 1992 | 1443426 |
| variation in responsiveness to trichinella spiralis infection in inbred rat strains. | an analysis of interstrain variation between 12 inbred and 4 congenic rat strains in the expression of immunity against trichinella spiralis is reported. all rat strains expressed strong rapid expulsion which resulted in the elimination of 88-98% of a challenge infection of muscle larvae. in contrast, substantial interstrain variation in the rate of adult worm expulsion in the primary infection was evident. by day 10 after infection, buf and yo strains had less than 50 worms left in the intestin ... | 1992 | 1437269 |
| genetic influences upon eosinophilia and resistance in mice infected with trichinella spiralis. | genetic influences upon host variation in eosinophilia and resistance to helminth infection, and the relationship between these parameters, were investigated in 7 inbred and 1 hybrid strains of mice infected with trichinella spiralis. clear strain-dependent variations were observed in the maximum peripheral blood, bone marrow and spleen eosinophilia attained in infected animals. swr, nih and sjl strains of mice all gave high responses to infection; four congenic strains sharing the b10 backgroun ... | 1992 | 1437268 |
| occurrence and characteristics of hypodense eosinophils in rats infected with trichinella spiralis. | hypodense eosinophils are observed in peripheral blood and tissue from patients with eosinophilia due to helminthic infections. in this study, the variation in eosinophil density was examined in rats during trichinella spiralis infection. hypodense eosinophils were observed in the peripheral blood in association with trichinella infection. in peritoneal fluid, which was representative of tissue fluid, a majority of eosinophils were hypodense regardless of the infection. during the course of tiss ... | 1992 | 1437239 |
| [the effect of specific therapy on the bone marrow reaction in experimental trichinelliasis]. | 1992 | 1435574 | |
| [the morphological changes in the organs of the lymphoid system in the experimental infection of mice with trichinella spiralis]. | the dynamics of morphological changes in central and peripheral organs of the lymphoid system of mice experimentally invaded with trichinella spiralis was studied. the immune response of the host was shown to have two phases including two peaks and the suppression period. the activation of the lymphoid organs was observed in earlier intestinal and muscular phases, and the suppression was noted in migration phase of invasion. | 1992 | 1435560 |
| [a morphometric study of the bone marrow of mice infected with trichinella spiralis and t. pseudospiralis]. | the study of cytograms of the marrow of mice with experimental t. spiralis and t. pseudospiralis invasion of 1 to 60 days revealed the marked activation of granulocytes and lymphoid cell proliferation with the much lower response of erythroid cells. the maximal response occurred at 21-35 days of invasion with subsequent diminution. in mice experimentally invaded with t. pseudospiralis the response of the marrow was less pronounced, but the period of active cell proliferation was prolonged. | 1992 | 1435529 |
| cyclosporin a in experimental trichinosis scanning electron microscopic study. | the surface morphology of adult and larvae of trichinella spiralis after cyclosporin a treatment was studied by scanning electron microscopy (sem) of fixed, dried and metal coated specimens. the earliest topographic changes revealed in adults after the drug treatment were in form of disorganization of the cuticle especially between the hypodermal pores which appeared somewhat thickened and irregular. other changes were in form of bullae formation covering the whole width of the adult surface. ot ... | 1992 | 1431295 |
| depletion of eosinophils by anti-il-5 monoclonal antibody treatment of mice infected with trichinella spiralis does not alter parasite burden or immunologic resistance to reinfection. | mechanisms of parasite killing by eosinophils are widely studied and are often implicated in mediating resistance to parasitic infection, especially in conjunction with specific antibodies. evidence for the eosinophil as an anti-parasite killer cell in vivo is limited and may not justify the belief that eosinophils engage and/or kill infective helminths. we reexamined this question in a mouse model of trichinosis in which antisera to eosinophils were previously used to show the requirement for e ... | 1992 | 1431133 |
| immunization against geographical isolates of trichinella spiralis in mice. | partially purified antigen preparations from six isolates of trichinella spiralis were used to immunize mice. immunogenicity of the antigens was assessed in terms of antibody and lymphocyte responses and ability to stimulate protective immunity against challenge. isolate antigens showed considerable cross-reactivity, and all elicited protective responses. two major patterns of immunizing ability could be distinguished: (a) isolates that immunized well against heterologous challenge and elicited ... | 1992 | 1428511 |
| immunogenetic and evolutionary influences on the host-parasite relationship. | immunogenetic aspects of the host-parasite relationship between the laboratory mouse and the parasitic nematode trichinella spiralis are reviewed. variation in the capacity of hosts to express an effective protective immunity is associated primarily with variation in inflammatory responsiveness. analysis of the contributions of t lymphocytes, cytokines, and myeloid precursor populations to variation in one particular inflammatory component, eosinophilia, is described. variation in parasite immun ... | 1992 | 1426489 |
| detection of circulating antigens in human trichinellosis. | a 'sandwich' enzyme-linked immunosorbent assay was established to detect circulating antigens of trichinella spiralis in human sera, its sensitivity and specificity was evaluated using 4 antigens (trichinella spiralis, trichuris trichiura, dirofilaria immitis and ascaris suum), and it was found to be sensitive and specific for t. spiralis antigen. samples of 347 individuals with suspected trichinellosis, who had eaten incompletely cooked bear meat containing larvae of t. spiralis, were examined. ... | 1992 | 1412659 |
| minimizing elisa background in the diagnosis of swine trichinellosis. | after confirming that long-term serum storage (frozen at -20 c for greater than 3 mo) causes optical density to drift upward, several modifications of an enzyme-linked immunosorbent assay (elisa) protocol were evaluated to identify a protocol that would reduce background in porcine sera tested for trichinellosis. modifications evaluated included blocking the antigen-coated elisa plate with sample diluent containing 10% bovine serum albumin (bsa) or 10% nonfat milk powder (bovine lacto transfer o ... | 1992 | 1403424 |
| boosted mucosal immune responsiveness in the rat intestine by actively transported hexose. | anaphylaxis-mediated intestinal fluid secretion was measured in trichinella spiralis- or ovalbumin-immunized rats challenged intraduodenally with t. spiralis somatic antigen (1 mg protein/0.5 ml saline) or ovalbumin (1 mg/0.5 ml saline), respectively. intestinal fluid volume was measured 30 minutes after challenge as an index of net secretion. challenge with the antigenic bolus containing 40 mmol/l d-glucose induced twice the fluid secretion as that induced by either antigen alone. l-glucose was ... | 1992 | 1397873 |
| [analysis of newborn larva of trichinella spiralis by immunoblot]. | newborn larva (nbl) antigens of trichinella spiralis were analysed by immunoblot, and were compared with the adult and muscle larva antigens. the sds-page patterns of nbl somatic constituents consisted of about 40 polypeptide bands, which were obviously different from those of adult and muscle larva. immunoblot analysis indicated that immunization with nbl could induce a stage specific immune response. the molecular weight of specific nbl antigens were 129, 120, 89, 87, 79, 74, 72, 64, 58, 43, 4 ... | 1992 | 1394901 |
| [changes in t lymphocytes and their subpopulations and igg in peripheral blood from mice infected with trichinella spiralis]. | acid alpha-naphthyl esterase (anae), a cytoplasmic marker, was used to identify the t lymphocytes and their subpopulations in peripheral blood of mice infected with trichinella spiralis, and dot-elisa was used to identify the serum igg antibody of the infected mice. the results showed that t lymphocytes increased on the d3 after infection, reaching the peak on d14, and then remained in number greater than normal up to d77. the spotted granular anae positive cells (help t cells, th) decreased and ... | 1992 | 1394900 |
| [chemistry and immunochemistry analysis of the soluble antigens of newborn larvae of trichinella spiralis]. | the soluble antigens of newborn larvae of trichinella spiralis were characterized in terms of molecular weight of protein, glycoprotein and lipoprotein contents, and immunochemistry. after the antigens were separated with sds-page and then followed by ultrasensitive silver staining, at least 40 bands of proteins were noted. correspondingly, 28 bands of glycoproteins and 9 bands of lipoproteins were revealed as the similar gels were stained by the hypersensitive periodic acid silver and nile's bl ... | 1992 | 1383119 |