Publications

thinopyrum bessarabicum: biochemical and cytological markers for the detection of genetic introgression in its hybrid derivatives with triticum aestivum l.	thinopyrum bessarabicum (2n = 2x = 14, jj) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. to accomplish this, diagnostic markers for detecting the presence of th. bessarabicum chromosomes in a wheat background have to be established. the c-banded karyotype of th. bessarabicum distinctly identifies individual th. bessarabicum chromosomes and separates them from tho ...	1993	24193484
stabilization of tetraploid triticale with chromosomes from triticum aestivum (abd)(abd)rr (2n = 28).	f1 hybrids with the genome constitution abderr (2n = 6x = 42) or abde(ab)rr (2n = 7x = 49), selected from crosses between either an octoploid triticum aestivum/thinopyrum elongatun amphiploid and tetraploid secale cereale (aabbddee x rrrr) or autoallohexaploid triticale [aabbddee x (ab)(ab)rrrr], were backcrossed to tetraploid triticale (ab)(ab)rr and selfed for six generations. thirty-three different tetraploid f6 progenies were karyotyped using c-banding. the aneuploidy frequency was 6.6% with ...	1993	24193483
genome analysis of intergeneric hybrids of apomictic and sexual australian elymus species with wheat, barley and rye: implication for the transfer of apomixis to cereals.	four hybrids were obtained between three australian elymus taxa and three cereal grains: wheat, rye, and barley. mean meiotic metaphase-i configurations were 41.14 i, 0.42 rod ii, 0.003 ring ii, and 0.01 iii for e. scabrus var 'plurinervis' x triticum aestivum (1 hybrid plant), 22.27 i, 2.63 rod ii, 0.06 ring ii, and 0.12 iii for e. scabrus var 'scabrus' x secale cereale (4 hybrid plants), and 26.65 i, 0.66 rod ii, 0.00 ring ii, and 0.01 iii for e. scabrus var 'plurinervis' x hordeum vulgare (13 ...	0	24193471
transformation of wheat (triticum aestivum l.) through electroporation of protoplasts.	protoplasts isolated from embryogenic suspension cultures of wheat (triticum aestivum cv. hartog) were electroporated in the presence of plasmid pemugn and/or pemupat, which contained the reporter gene gus and selectable marker gene bar, respectively. under optimised electroporation conditions, up to 0.9% of viable protoplasts displayed gus activity two days after electroporation. to select for phosphinothricin (ppt) resistant colonies, electroporated protoplasts were incubated for six weeks in ...	1994	24192893
organ-specific phosphorus-allocation patterns and transcript profiles linked to phosphorus efficiency in two contrasting wheat genotypes.	recent studies have identified genotypic variation in phosphorus (p) efficiency, but rarely have the underlying mechanisms been described at the molecular level. we demonstrate that the highly p-efficient wheat (triticum aestivum l.) cultivar chinese 80-55 maintains higher inorganic phosphate (pi ) concentrations in all organs upon pi withdrawal in combination with higher pi acquisition in the presence of pi when compared with the less-efficient cultivar machete. these findings correlated with d ...	2014	24191900
polymorphism of omega-gliadins in durum wheat as revealed by the two-step apage/sds-page technique.	polymorphism of omega-gliadins was studied in 243 durum wheats from 27 countries using the two-step one-dimensional apage/sds-page technique. a total of 12 bands of different mobility were observed, and four of them were found to be different from those previously detected by khelifi et al. (1992) in bread wheat. fifteen alleles, six coded by the gli-a1 locus and nine coded by the gli-b1 locus, were identified, accounting for 19 different electrophoretic patterns. seven new alleles were detected ...	1994	24190535
enhancement of the salt tolerance of triticum turgidum l. by the kna1 locus transferred from the triticum aestivum l. chromosome 4d by homoeologous recombination.	durum wheat, triticum turgidum l. (2n= 4x=28, genome formula aabb) is inferior to bread wheat, t. aestivum l. (2n=6x=42, genome formula aabbdd), in the ability to exclude na(+) under salt strees, in the ratio of the accumulated k(+) to na(+) in the leaves under salt stress, and in tolerance of salt stress. previous work showed that chromosome 4d has a major effect on na(+) and k(+) accumulation in the leaves of bread wheat. the 4d chromosome was recombined with chromosome 4b in the genetic backg ...	1994	24190475
molecular analysis of plants regenerated from embryogenic cultures of wheat (triticum aestivum l.).	total dnas of plants regenerated from immature embryo-derived 2-month-old embryogenic calli of wheat (cultivars florida 302, chris, pavon, rh770019) were probed with six maize mitochondrial genes (atpa, atp6, apt9, coxi, coxii, rrn18-rrn5), three hypervariable wheat mitochondrial clones (k', k3, x2), five random pearl millet mitochondrial clones (4a9, 4d1, 4d12, 4e1, 4e11) and the often-used wheat nor locus probe (pta71), in order to assess the molecular changes induced in vitro. in addition, pr ...	1994	24190468
the genetic characterisation of novel multi-addition doubled haploid lines derived from triticale x wheat hybrids.	two novel 46-chromosome doubled haploid lines, w66 and m17, derived from separate hexaploid triticale x bread wheat crosses, were characterised using cytological and biochemical markers. both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively. by examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n=46 in 74.2-85.5% of cells. however, over selfed generations, the rye chro ...	1993	24190346
a self-fertile trigeneric hybrid,triticum aestivum ×agropyron michnoi ×secale cereale.	trigeneric hybrids between the (triticum aestivum ×agropyron michnoi) f1 (cm, 2n=5x=35; abdpp) and two winter rye (secale cereale l., 2n=2x=14; rr) cultivars, 'wugong 774' and 'ar-132', were synthesized. such trigeneric hybrids could be used to transfer resistance genes for powdery mildew from rye to cm and subsequently to common wheat and to identify (1) the effects of the p genome ofagropyron on the self-fertility of the hybrids and (2) the differences in genetic background between rye cultiva ...	1993	24190263
seed protein and isozyme variations in triticum tauschii (aegilops squarrosa).	sixty triticum tauschii (aegilops squarrosa, 2n=2x=14, dd) accessions were evaluated for the variability of high-molecular-weight (hmw) glutenins, gliadins and isozymes of seed esterase, β-amylase and glucose-phosphate isomerase. wide variability was observed for hmw-glutenins and gliadins. the implications of unique hmw-glutenin alleles for quality parameters are discussed. isozyme evaluations indicated more variability for the est-d (t) 5 locus as compared to the est-d5 of bread-wheat. the pol ...	1993	24190222
expression of individual hmw glutenin subunit genes of wheat (triticum aestivum l.) in relation to differences in the number and type of homoeologous subunits and differences in genetic background.	the amount of individual high-molecular-weight (hmw) glutenin subunits of bread-wheat has been studied in relation to variation at homoeologous loci and in the general genetic background. the relationships between glu-1 loci have been studied using nearisogenic lines (nils) of the variety sicco and in the progenies of two crosses. substitution of the sicco glu-d1 allele by a null-allele resulted in higher amounts of the homoeologous subunits. the presence of a glu-a1 nullallele did not have a no ...	1993	24190214
recombination mapping of gli-5, a new gliadin-coding locus on chromosomes 1a and 1b in common wheat.	inheritance studies of gliadin loci on chromosomes 1a and 1b were carried out in the progeny from crosses between cv "salmone" and six other common wheat varieties. the map distance between the rg-1 locus for glume colour and the gliadin locus gli-b1 on the satellite of chromosome 1b was calculated as 2.0±0.6 cm. an additional gliadin locus, gli-b5, was mapped between gli-b1 and rg-1, 1.4 cm from the former. a genetic distance of 1.8±0.4 cm was obtained between the hg-1 locus for hairy glumes an ...	1993	24190202
rflp-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (triticum aestivum l.).		1993	24190195
restriction fragment patterns of chloroplast and mitochondrial dna of dasypyvum villosum (l.) candargy and wheats.	to elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) dnas isolated from two different accessions of dasypyrum villosum (l.) candargy were compared with those of tetraploid wheat (triticum durum desf., pi265007), hexaploid wheat (triticum aestivum l., cv chinese spring), aegilops longissimum (s. and m., in muschli) bowden and hordeum vulgare l. t. aestivum and t. durum had identical restriction pat ...	1993	24190190
liquid chromatography-diode array detection to study the metabolism of glufosinate in triticum aestivum t-590 and influence of the genetic modification on its resistance.	the resistance to glufosinate of two lines-genetically modified (gm) and unmodified (t-590 and t-549, respectively)-of triticum aestivum has been studied. in the gm line, the bar gene was introduced to increase the resistance to glufosinate. experiments in a controlled growth chamber showed that line t-590 presented a high resistance to glufosinate with an ed50 value of 478.59 g active ingredient per hectare (g ai ha(-1)) versus 32.65 g ai ha(-1) for line t-549. the activity of glutamine synthet ...	2013	24189348
functional, structural and phylogenetic analysis of domains underlying the al sensitivity of the aluminum-activated malate/anion transporter, taalmt1.	triticum aestivum aluminum-activated malate transporter (taalmt1) is the founding member of a unique gene family of anion transporters (almts) that mediate the efflux of organic acids. a small sub-group of root-localized almts, including taalmt1, is physiologically associated with in planta aluminum (al) resistance. taalmt1 exhibits significant enhancement of transport activity in response to extracellular al. in this study, we integrated structure-function analyses of structurally altered taalm ...	2013	24188189
short-term effects of nitrate on sucrose synthesis in wheat leaves.	experiments were carried out with fully expanded leaves from three-week-old seedlings of wheat (triticum aestivum l.) raised without no 3 (sup-) . nitrate was supplied to the leaves through the transpiration stream in the light. uptake of no 3 (sup-) was linear with no 3 (sup-) concentrations from 0 to 80 mm in the solution. net sucrose synthesis showed inverse relationships versus nitrate uptake, assimilation, and accumulation, with correlation coefficients close to 1. by contrast, no alteratio ...	1991	24186279
metaphase i-bound arms frequency and genome analysis in wheat-aegilops hybrids. 3. similar relationships between the b genome of wheat and s or s (l) genomes of ae. speltoides, ae. longissima and ae. sharonensis.	the meiotic behaviour of triticum aestivum × aegilops speltoides, t. aestivum × ae. sharonensis and t. aestivum × ae. longissima tetraploid hybrids (genome constitution abds, abds (l) , and abds (l) , respectively) has been analysed by the c-banding technique. of the six types of pairing normally occurring, at metaphase i three were recognized: a-d, ad-bs/ad-bs (l) and b-s/b-s (l) . the relative order observed in the low pairing hybrid, a-d> b-s (l) >ad-bs (l) , as well as that found in high-pai ...	1994	24186260
genetic analysis of carbon isotope discrimination and agronomic characters in a bread wheat cross.	carbon isotope discrimination (δ) has been suggested as a selection criterion to improve transpiration efficiency (w) in bread wheat (triticum aestivum l.). cultivars 'chinese spring' with low a (high w) and 'yecora rojo' with high δ (low w) were crossed to develop f1, f2, bc1, and bc2 populations for genetic analysis of δ and other agronomic characters under well-watered (wet) and water-stressed (dry) field conditions. significant variation was observed among the generations for δ only under th ...	1994	24186257
genetic diversity in european wheat and spelt breeding material based on rflp data.	fifty-two winter wheat (triticum aestivum l.), nine spring wheat, and 20 spelt (triticum spelta l.) lines representing part of the european breeding germplasm, were assayed for rflps (restriction fragment length polymorphisms) with 56 wheat dna clones and two barley cdna clones. objectives of this study were to (1) determine the level of variation for rflps in the wheat and spelt breeding lines, (2) characterize the genetic diversity within the european winter wheat germplasm, and (3) evaluate t ...	1994	24186253
pcr analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality.	good or poor wheat bread-making quality is associated with two allelic pairs at theglu-d1 complex locus, designated 1dx5-1dy10 and 1dx2-1dy12, respectively. the polymerase chain reaction (pcr) verified the presence of the hmw-glutenin 1dx5 gene from genomic dna extracted from part of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. this easy, quick, and non-destructive pcr-based approach is proposed as a very efficient and safe alter ...	1994	24186174
an nadh nitrate reductase gene copy appears to have been deleted in barley.	cultivated barley,hordeum vulgare l., has a single nadh nitrate reductase (nr) gene while diploid wheat,triticum monococcum, and cultivated hexaploid wheat,triticum aestivum l., have two nadh nr genes. to determine whether the nadh nr gene was duplicated since the divergence oftriticum fromhordeum or was deleted from barley, thet. monococcum nadh nr gene heme-hinge regions were sequenced and compared with the barley nadh nr gene sequence. sequence identity and phylogenetic analyses showed that o ...	1994	24186114
chromosome assignment of four photosynthesis-related genes and their variability in wheat species.	copy numbers of four photosynthesis-related genes, phya, ppc, rbcs and lhcb1 (*)1, in wheat genomes were estimated by slot-blot analysis, and these genes were assigned to the chromosome arms of common wheat by southern hybridization of dna from an aneuploid series of the cultivar chinese spring. the copy number of phya was estimated to be one locus per haploid genome, and this gene was assigned to chromosomes 4al, 4bs and 4ds. the ppc gene showed a low copy number of small multigenes, and was lo ...	1994	24186023
the influence of the rye genome on the accumulation of hsp18 and hsp70 transcripts in a wheat genetic background.	the influence of the rye genome on the accumulation of hsp18 and hsp70 transcripts in a wheat genetic background was examined in the wheat/rye hybrid triticale (triticum aestivum cv chinese spring x secale cereale cv imperial). to quantify the amount of transcript accumulation in wheat, rye, triticale, and in the disomic and the ditelosomic rye addition lines to wheat, we used two independant methods, namely (1) northern dot-blot hybridizations and (2) an exami-nation of the in-vitro translation ...	1994	24186010
pcr analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the glu-d1 locus.	genes encoding high-molecular-weight (hmw) glutenin subunits, present in bread-wheat lines and cultivars, were studied by rflp (restriction fragment length polymorphism) and pcr (polymerase chain reaction) analyses. in particular, allelic subunits of the x-or y-type, encoded at the glu-d1 locus present on the long arm of chromosome 1d, were investigated. the variation in size, observed in different allelic subunits, is mainly due to variation in the length of the central repetitive domain, typic ...	1994	24185923
transfer of ph (i) genes promoting homoeologous pairing from triticum speltoides to common wheat.	diploid-like chromosome pairing in polyploid wheat is controlled by several ph (pairing homoeologous) genes with major and minor effects. homoeologous pairing occurs in either the absence of these genes or their inhibition by genes from other species (ph (i) genes). we transferred ph (i) genes from triticum speltoides (syn aegilops speltoides) to t. aestivum, and on the basis of further analysis it appears that two duplicate and independent ph (i) genes were transferred. since ph (i) genes are e ...	1994	24185888
effect of gliadins and hmw and lmw subunits of glutenin on dough properties in the f6 recombinant inbred lines from a bread wheat cross.	the storage proteins of 64 f2-derived f6 recombinant inbred lines (rils) from the bread wheat cross 'prinqual'/'marengo' were analyzed. parents differed at four loci: gli-b1 (coding for gliadins), glu-b1 (coding for hmw glutenin subunits), glu-a3/gli-a1 (coding for lmw glutenin subunits/gliadins) and glu-d3 (coding for lmw glutenin subunits). the effect of allelic variation at these loci on tenacity, extensibility and dough strength as measured by the chopin alveograph was determined. allelic di ...	1994	24185886
wheat chromosome 2d carries genes controlling the activity of two dna-degrading enzymes.	dna-degrading enzymes of 24.0 kda and 27.0 kda were observed to have different activities in two common wheat (triticum aestivum l.) cultivars, 'wichita' and 'cheyenne'. a substrate-based sds-page assay revealed that these two enzymes were much more active in 'wichita' than in 'cheyenne'. genes controlling the activities of these two enzymes were localized on chromosome 2d by testing dna-degrading activities in reciprocal chromosome substitution lines between 'wichita' and 'cheyenne'. while the ...	1994	24185878
factors affecting the establishment and maintenance of embryogenic callus and suspension cultures of wheat (triticum aestivum l.).	improved suspension cell culture systems are needed to facilitate the application of recombinant dna technology for wheat germplasm enhancement. this study evaluated three wheat (triticum aestivum l.) cultivars, and the effects of medium basal salts, 2,4-d, sucrose, and l-proline concentrations on the establishment of rapidly growing and highly embryogenic callus and suspension cultures. percent embryogenic calli was visually estimated and verified with light and scanning electron microscopy. th ...	1995	24185782
comparison of developmental stages of inflorescence for high frequency plant regeneration in triticum aestivum l. and t. durum desf.	whole immature inflorescences at 4 different developmental stages (0.5, 1.0, 1.5, 2.0 cm in size) of different genotypes of triticum aestivum and t. durum were cultured to see the morphogenetic responses on murashige and skoog's (ms) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-d) (2.5 mg/l). very young inflorescences 0.5 and 1.0cm long formed embryogenic callus from their entire surface while 1.5 and 2.0 cm long inflorescences formed embryogenic callus from the basal spikelets a ...	1995	24185781
in vitro development of globular zygotic wheat embryos.	we have established in vitro culture conditions for globular zygotic wheat embryos (triticum aestivum l.). their nutritional requirements have been systematically investigated. the initial sucrose concentration, as well as the sucrose concentration during the culture, a 6-benzylaminopurine supplement, the use of nitrates and ammonium as nitrogen source have a major influence on the embryo development. proline has an inhibitory effect on the germination. a double layer system with different media ...	1995	24185773
electro-fused isolated wheat (triticum aestivum l.) gametes develop into multicellular structures.	the electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. on average the fusion frequency was 30% but under optimal conditions it was possible to reach as much as 55%. two days after electric fusion 60% of the fusion products started to divide, 88.5% of them forming multicellular structures and in a few cases microcalluses. the culture of single unfertilized egg cells with or withou ...	1995	24185771
identification of a wheat (triticum aestivum) cell line lacking a specific divalent cation requirement.	a wheat (triticum aestivum l.) cell line, derived from anther culture of an f1 hybrid, has exogenous ca(2+), to that of calcium-dependent cells grown on complete medium. the calcium-independent cell line has been grown in the absence of ca(2+) for more than 1.5 years. the cell line grew at a rate similar to that on complete medium for up to 12 weeks, if supplied with any one of the divalent cations, ca(2+), mg(2+), mn(2+), zn(2+), cu(2+) or co(2+), but declined and appeared necrotic when all 6 o ...	1995	24185448
field study on the uptake and translocation of perfluoroalkyl acids (pfaas) by wheat (triticum aestivum l.) grown in biosolids-amended soils.	field experiments were performed to evaluate the uptake and translocation of perfluoroalkyl acids (pfaas) in wheat (triticum aestivum l.) grown in soils amended with biosolids at different rates. nine perfluorocarboxylic acids (pfcas) and three perfluorosulfonic acids (pfsas) were detected in the soils and wheat tissues. total concentrations of pfaas in the soils and wheat root, straw, husk and grain increased with increasing application of biosolids. pfca concentrations in grain increased logar ...	2014	24184376
accelerated production of transgenic wheat (triticum aestivum l.) plants.	we have developed a method for the accelerated production of fertile transgenic wheat (triticum aestivum l.) that yields rooted plants ready for transfer to soil in 8-9 weeks (56-66 days) after the initiation of cultures. this was made possible by improvements in the procedures used for culture, bombardment, and selection. cultured immature embryos were given a 4-6 h pre-and 16 h post-bombardment osmotic treatment. the most consistent and satisfactory results were obtained with 30 μg of gold par ...	1996	24178645
effect of anoxia on starch breakdown in rice and wheat seeds.	the capabilities of rice (oryza sativa l.) and wheat (triticum aestivum l.) seeds (caryopses) to degrade starchy reserves present in the endosperm tissue were compared under anaerobic conditions. the results showed that rice, a species highly tolerant to anoxia, can readily break down starch under anaerobiosis concomitant with germination, while wheat does not germinate and fails to degrade starch present in the endosperm. this clearly distinct behavior is likely the consequence of the successfu ...	1992	24178396
aluminum effects on the kinetics of calcium uptake into cells of the wheat root apex : quantification of calcium fluxes using a calcium-selective vibrating microelectrode.	the effects of aluminum on the concentration-dependent kinetics of ca(2+) uptake were studied in two winter wheat (triticum aestivum l.) cultivars, al-tolerant atlas 66 and al-sensitive scout 66. seedlings were grown in 100 μm cacl2 solution (ph 4.5) for 3 d. subsequently, net ca(2+) fluxes in intact roots were measured using a highly sensitive technique, employing a vibrating ca(2+)-selective microelectrode. the kinetics of ca(2+) uptake into cells of the root apex, for external ca(2+) concentr ...	1992	24178332
effect of long-term photoinhibition on growth and photosynthesis of cold-hardened spring and winter wheat.	the effect of repeated exposure to high light (1200 μmol · m(-2) · s(-1) photosynthetic photon flux density, ppfd) at 5° c was examined in attached leaves of cold-grown spring (cv. katepwa) and winter (cv. kharkov) wheat (triticum aestivum l.) over an eight-week period. under these conditions, kharkov winter wheat exhibited a daily reduction of 24% in fv/fm (the ratio of variable to maximal fluorescence in the dark-adapted state), in contrast to 41% for cold-grown katepwa spring wheat. both cult ...	1992	24178327
cell shape and localisation of ice in leaves of overwintering wheat during frost stress in the field.	wheat leaf pieces were excised and freeze-fixed in the field, preparatory to low-temperature scanning electron microscopy to study distribution of ice within leaf blades, and associated cell shapes, during natural frosts. pieces of leaf blades from wheat plants (triticum aestivum l. 7942h1-20-8) overwintering in indiana, usa (january, 1991), were excised and immediately freeze-fixed by manually plunging in melting freon. cells in controls were turgid and extracellular ice was absent. the leaves ...	1992	24178321
rflp markers associated with sr22 and recombination between chromosome 7a of bread wheat and the diploid species triticum boeoticum.	analysis of the bread wheat variety schomburgk, and related lines in its pedigree, identified rflp markers associated with the segment of chromosome 7a carrying the sr22 gene derived from the diploid species t. boeoticum. the distribution of the rflp markers indicated that at least 50% of 7as and 80% of 7al in schomburgk is of t. boeoticum origin. evaluation of five sets of nearisogenic lines, backcross lines in 20 different genetic backgrounds and an f2 population segregating for sr22 demonstra ...	1994	24178122
quantitative-genetic analysis of reciprocal crosses between a winter and a spring cultivar of common wheat (triticum aestivum l.).	the winter wheat cultivar sakhalin (parent a) and the spring wheat cultivar khush-hal (parent b), assumed to be both nuclear-genetically and plasmotypically different, were reciprocally crossed and the f1 generation of the reciprocals backcrossed to either parent. the populations (axb)f1, (axb)f2, [(axb)f1xa]f1 and [(axb)f1xb]f1, and their reciprocals (bxa)f1, (bxa)f2, [(bxa)f1xa]f1 and [(bxa)f1xb]f1, assumed to have the plasmotypes of the parents a and b respectively, were spring sown in the sa ...	1994	24178121
diallel analysis to predict heterosis and combining ability for grain yield, yield components and bread-making quality in bread wheat (t. aestivum).	combining ability for grain yield, yield components, and several agronomic and qualitative traits, was studied in a seven-parent diallel cross. the 21 f1 hybrids and the seven parental cultivars were grown in replicated plot trials sown at normal seed density in three locations in the years 1992 and 1993. the effects of general combining ability (gca) were highly significant for all the traits measured with the exception of seeds per spikelet, while the specific combining ability (sca) effects w ...	1994	24178113
association of a dna marker with hessian fly resistance gene h9 in wheat.	the hessian fly [mayetiola destructor (say)] is a major pest of wheat (triticum aestivum l.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. to-date, 25 hessian fly resistance genes, designated h1 to h25, have been identified in wheat. with near-isogenic wheat lines differing for the presence of an individual hessian fly resistance gene, in conjunction with random amplified polymorphic dna (rapd) analysis and denaturing ...	1994	24178111
a partial genome assay for quantitative trait loci in wheat (triticum aestivum) using different analytical techniques.	f1 plants between two intervarietal chromosome substitution lines of european spring wheat varieties, 'sicco' ('chinese spring 5b') and 'highbury' ('chinese spring 5b'), were used to produce 114 doubled haploid lines, 45 by the hordeum bulbosum technique and 69 by anther culture. these two sets of lines were characterized for variation at a range of morphological, isozyme and rflp marker loci, and genetic maps were developed with emphasis on chromosomes 6b, 7a, 7b and 7d. a subset of lines, scor ...	1994	24178019
long-term steady-state labelling of wheat plants by use of natural (13)co 2/ (12)co 2 mixtures in an open, rapidly turned-over system.	a photosynthate labelling method is presented which takes advantage of the natural difference in carbon-isotope composition (δ carbon-isotope composition of a sample is conventionally presented as δ (‰) = [(rp/rs) -1] · 1000, where rp and rs are the molar abundance ratios, (13)c/(12)c, of the sample and of the standard (pdb carbonate), respectively ) which exists between atmospheric co2 (δ≈-8‰) and commercially available compressed co2. carbon dioxide with δ-4.0 and -27.9%., respectively, has be ...	1992	24177977
linkages between common wheat yields and climate in morocco (1982-2008).	in morocco, wheat production shows a high inter-annual variability due to uncertain rainfall. in view of the importance of this resource to the country's economy, it is important to gain a better understanding of the natural large-scale climate oscillation governing this variability. in this study, we analyzed de-trended (1) time series of common wheat yields (1983-2008) from 11 agricultural provinces that account for 80% of national wheat production; (2) monthly rainfall and 10-day temperature ...	2014	24177944
an n-band marker for gene lr18 for resistance to leaf rust in wheat.	the leaf rust resistance gene, lr18, of common wheat cultivars has been derived from triticum timopheevi and is located on chromosome arm 5bl. chromosome banding (n-banding) analyses revealed that in the wheat cultivars carrying lr18 that were examined, which had been bred in 6 different countries, chromosome arm 5bl possessed a specific terminal band not carried by their susceptible parental cultivars. it was suggested that this terminal n-band was introduced from t. timopheevi together with lr ...	1994	24177942
hybrids and backcross progenies between wheat (triticum aestivum l.) and apomictic australian wheatgrass [elymus rectisetus (nees in lehm.) a. löve & connor]: karyotypic and genomic analyses.	wheat (triticum aestivum l., 2n = 6x = 42, aabbdd) florets were emasculated and pollinated using two apomictic wheatgrass [elymus rectisetus (nees in lehm.) a. love & connor, 2n = 6x = 42, ssyyww] accessions, one of which produces 2n pollen. a 2n = 42 (bii) hybrid and four 2n = 63 (b iii) hybrids were obtained. the spike morphology of the b ii hybrid was intermediate to that of its parents. the pollen mother cells (pmcs) of this hybrid contained on average 38.361 and 1.62 ii, which was consisten ...	1994	24177936
transformation of protoplasts and intact cells from slowly growing embryogenic callus of wheat (triticum aestivum l.).	a procedure for culturing protoplasts from slowly growing embryogenic calli of wheat was developed. the procedure was dependent on the ability to isolate large numbers of culturable protoplasts from slowly growing embryogenic callus. approximately 68% of the isolated protoplasts divided, and 22% formed colonies; of the latter, 67% continued to proliferate. plating efficiency was reduced when protoplasts were transformed by polythylene glycol, electroporation, and/or agrobacterium. intact cells w ...	1994	24177933
durum wheat haploid production using maize wide-crossing.	while anther culture or pollinations with hordeum bulbosum have provided suitable methods for haploid production in bread wheat, they have been largely unsuccessful in durum wheat. pollinations with maize were used in an attempt to produce haploid seedlings and, from these, fertile doubled haploids of durum wheats. moreover, the effect of various concentrations and combinations of a synthetic auxin, 2, 4-dichlorophenoxyacetic acid (2,4-d), kinetin, and an ethylene inhibitor, silver nitrate (agno ...	1994	24177930
chromosomal location of genes controlling short-term and long-term somatic embryogenesis in wheat revealed by immature embryo culture of aneuploid lines.	the expression of essential genes during somatic embryogenesis can be analysed by inducing aneuploid cells to undergo embryogenesis during immature embryo culture and then determining whether defects occur. triticum aestivum disomic and aneuploid stocks, including 36 ditelosomics and 7 nullitetrasomic 'chinese spring' wheats, were compared for their ability to undergo somatic embryogenesis after 2 months of in vitro immature embryo culture. their regeneration capacity was observed after 4 and 14 ...	1994	24177852
sister chromatid exchanges in cultured immature embryos of wheat species and regenerants.	immature embryos of triticum aestivum (ten cultivars and lines), t. durum, t. dicoccum and t. monococcum were cultured in vitro on ms medium supplemented with 1 or 2 mg/l of 2,4-d and 20 or 30 g/l of sucrose for 3 days and processed to score sister chromatid exchanges (sces) per chromosome. media components affect dna replication from the start of the culture. the sce frequencies were dependent on the genotype and were not correlated with the degree of ploidy. they increased after doubling of th ...	1994	24177843
determination of the frequency of wheat-rye chromosome pairing in wheat x rye hybrids with and without chromosome 5b.	genomic in-situ hybridization (gish) was used to determine the amount of wheat-rye chromosome pairing in wheat (triticum aestivum) x rye (secale cereale) hybrids having chromosome 5b present, absent, or replaced by an extra dose of chromosome 5d. the levels of overall chromosome pairing were similar to those reported earlier but the levels of wheat-rye pairing were higher than earlier determinations using c-banding. significant differences in chromosome pairing were found between the three genot ...	1994	24177837
waxy protein deficiency and chromosomal location of coding genes in common wheat.	deficiency of the wheat waxy (wx) proteins (wx-a1, wx-b1 and wx-d1) was studied in 1,960 cultivars derived from several countries. gel electrophoretic analyses revealed that the null allele for the wx-a1 protein occurred frequently in korean, japanese and turkish wheats but was relatively rare in cultivars from other countries and regions. about 48% of the wheats deficient for the wx-b1 protein were from australia and india. one chinese cultivar lacked the wxd1 protein. while 9 japanese cultivar ...	1994	24177825
identification of wheat-agropyron cristatum monosomic addition lines by rflp analysis using a set of assigned wheat dna probes.	a non-radioactive digoxigenin-labelled dna method was used successfully to identify rflp markers in 54 triticum aestivum cv 'chinese spring' - agropyron cristatum (2n=28, genome pppp) p-genome monosomic addition lines. southern analysis using a set of 14 dna probes identifying each homoeologous chromosome arm, combined with two restriction enzymes hindiii and ecori, indicated that six a. cristatum chromosomes (1p, 2p, 3p, 4p, 5p and 6p) and five a. cristatum chromosome arms (2ps, 2pl, 5pl, 6ps a ...	1994	24177772
silicon carbide fiber-mediated dna delivery into cells of wheat (triticum acstivum l.) mature embryos.	we have demonstrated that foreign dna can be delivered into cells of mature embryos of wheat (triticum aestivum l.) using silicon carbide fibers (scf). the highest transient expression of thegusa (gus) gene was detected when dry embryos were vortexed for 10-30 min in a scf-dna solution containing 90-120 g/l of sucrose. up to 100 (on average 20-40) blue expression units per embryo were observed. scutellum side and epiblast of the intact wheat embryos are preferentially transformed. when embryos w ...	1996	24177538
production and genetic characterization of near-isogenic lines in the bread-wheat cultivar alpe.	two biotypes of the bread-wheat cultivar alpe were shown to possess contrasting alleles at each of the glutenin (glu-b1, glu-d1, glu-b3 and glu-d3) and gliadin (gli-b1 and gli-d1) loci on chromosomes 1b and 1d. fourteen near-isogenic lines (nils) were produced by crossing these biotypes and used to determine the genetic control of both low-molecular-weight (lmw) glutenin subunits and gliadins by means of one-dimensional or two-dimensional electrophoresis. genes coding for the b, c and d groups o ...	1995	24174023
conversion of a rapd-generated pcr product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat.	bulk segregant analysis was used to obtain a random amplified polymorphic dna (rapd) marker specific for the rye chromosome arm of the 1bl.1rs translocation, which is common in many high-yielding bread wheat varieties. the rapd-generated band was cloned and end-sequenced to allow the construction of a pair of oligonucleotide primers that pcr-amplify a dna sequence only in the presence of rye chromatin. the amplified sequence shares a low level of homology to wheat and barley, as judged by the lo ...	1995	24174021
genetic control for resistance to leaf rust in wheat-agropyron lines: agro 139 and agro 58.	leaf rust resistance lines of triticum aestivum carry highly effective lr genes from agropyron intermedium (host) beauv. this agro 58 and agro 139 resistance segregated independently of agropyron leaf-rust resistance genes lr-19, lr-24 and lr-9 from ae. umbellulata. monosomic analysis showed that the lr gene in agro 139 was incorporated into wheat chromosome 6d. c-banding analysis could not determine the c-banding pattern of a. intermedium in wheat -agropyron lines agro 58 and agro 139. it is as ...	1995	24174018
molecular identification of powdery mildew resistance genes in common wheat (triticum aestivum l.).	rflp markers for the wheat powdery mildew resistance genes pm1 and pm2 were tagged by means of near-isogenic lines. the probe whs178 is located 3 cm from the pm1 gene. for the powdery mildew resistance gene pm2, two markers were identified. the linkage between the pm2 resistance locus and one of these two probes was estimated to be 3 cm with a f2 population. both markers can be used to detect the presence of the corresponding resistance gene in commercial cultivars. "bulked segregant analysis" w ...	1995	24174015
visualization of secale cereale dna in wheat germ plasm by fluorescent in situ hybridization.	homozygous wheat/rye (1bl/1rs or 1as/ 1rl) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses. detecting the presence of rye at the chromosome level is feasible by c-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analyzing smaller dna introgressions has greater significance. as a first step we have applied th ...	1995	24174014
use of winter wheat x triticum tauschii backcross populations for germplasm evaluation.	the wild diploid goatgrass, triticum tauschii (coss.) schmal., is an important source of genes for resistance to both diseases and insects in common wheat (triticum aestivum l.) we have evaluated grain yield, kernel weight, protein concentration, and kernel hardness of 641 bc2 f1-derived families from direct crosses involving four t. aestivum cultivars and 13 t. tauschii accessions over 2 years and at two kansas, usa, locations. on average, t. tauschii germplasm depressed grain yield and increas ...	1995	24173953
diallel analysis of the latent period of stripe rust in wheat.	a half diallel was made amongst five wheat (triticum aestivum l.) genotypes of which one was susceptible, while the others had adult-plant resistance, to stripe rust (puccinia striiformis west.). the five parent and ten f1 progeny were grown in the glasshouse and were inoculated with three rust pathotypes at the seedling stage. the latent period was measured on the first leaf. two procedures were used to analyze the half diallel. both methods showed that the average effects of alleles were of mu ...	1995	24173940
genetic variation at storage protein-coding loci of common wheat (cv 'chinese spring') induced by nitrosoethylurea and by the cultivation of immature embryos in vitro.	electrophoretic patterns of seed storage proteins, the high-molecular-weight glutenins and gliadins, were studied in 468 plants of the common wheat cultivar 'chinese spring' regenerated from callus culture of immature embryos, in 115 plants grown from seeds treated with nitrosoethylurea and in 260 control plants. from 5 to 21 single grains were analysed from each plant. in these three groups, the frequency of inherited mutations causing the loss of all proteins controlled by a locus (null-mutati ...	1995	24173927
application of two microsatellite sequences in wheat storage proteins as molecular markers.	in eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic pcr-based genetic markers. in hexaploid bread wheat, a large-genome inbreeding species with low levels of rflp, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to pcr-based markers. these were shown to be genome-specific and displayed high levels of variation. these characteristics make them especially suitable for ...	1995	24173898
chromosomal control of the tolerance of gradually and suddenly imposed salt stress in the lophopyrum elongatum and wheat, triticum aestivum l. genomes.	the facultatively halophytic lophopyrum elongatum, closely related wheat, triticum aestivum, and their amphiploid tolerate salt stress better if they are gradually exposed to it than if they are suddenly stressed. lophopyrum elongatum has greater tolerance of both forms of salt stress than wheat, and its genome partially confers this tolerance on their amphiploid. chromosomal control of the tolerance of both stress regimes in the l. elongatum and wheat genomes was investigated with disomic and d ...	1995	24173895
rflp patterns of gliadin alleles in triticum aestivum l.: implications for analysis of the organization and evolution of complex loci.	a correspondence between rflp patterns and gliadin alleles at the gli-1 and gli-2 loci was established in a set of 70 common wheat (t.aestivum l.) cultivars using γ-gliadin (k32) and α-gliadin (ptu1) specific probes. all gli-b1 and gli-d1 alleles which differed in encoded γ-gliadins showed definite rflp patterns after hybridization with the k32 probe. two groups of gli-b1 alleles, gli-b1b-like and gli-b1e-like, were identified, and these could originate from distinct genotypes of the presumptive ...	1995	24173888
standard karyotype of triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in common wheat.	a standard karyotype and a generalized idiogram of triticum umbellulatum (syn. aegilops umbellulata, 2n = 2x = 14) was established based on c-banding analysis of ten accessions of different geographic origin and individual t. umbellulatum chromosomes in t. aestivum - t. umbellulatum chromosome addition lines. monosomic (ma) and disomic (da) t. aestivum - t. umbellulatum chromosome addition lines (da1u = b, da2u = d, ma4u = f, da5u = c, da6u = a, da7u = e = g) and telosomic addition lines (da1us, ...	1995	24173798
quality and biochemical effects of a ibl/irs wheat-rye translocation in wheat.	wheat (triticum aestivum l.) breeders world-wide have used rye (secale cereale l.) as a source of genes for agronomic improvement. the 1bl/1rs wheat-rye chromosomal translocation derived from the russian cultivars 'kavkaz' and 'aurora' has been among the most common means of accessing useful rye genes. unfortunately, deleterious wheat quality effects are often associated with the presence of 1rs. the identification of genetic backgrounds capable of alleviating the deleterious effects of 1rs is c ...	1995	24173790
thinopyrum distichum addition lines: production, morphological and cytological characterisation of 11 disomic addition lines and stable addition-substitution line.	plants of the partial amphiploid inia 66/thinopyrum distichum (2n = 70)//inia 66 (2n = 56) were used as male parents in crosses with the monosomic series in the common wheat cultivar inia 66. the genome and homoeologous group of the monosomic used in the cross affected the distribution of chromosome number of the progeny plants in the f2 and f4. meiosis in the pollen mother cells of the b1f7 partial amphiploids was not stable, and not different from that of the b1f1 in which univalents and multi ...	1995	24173781
wheat lines monogenic for resistance to stem rust from the wheat cultivar 'waldron'.	the triticum aestivum l. cultivar 'waldron' has long lasting resistance to most north american stem rust (puccinia graminis pers.:pers. f. sp. tritici eriks. and e. henn.) isolates. the objective of this research was to develop wheat lines monogenic for resistance to stem rust from 'waldron' using allelism tests and tests for reaction to a series of ten stem rust cultures having a range of virulences. twelve lines homozygous for single resistance genes were selected as parents of a diallel cross ...	1995	24173079
chromosomal location of genes for stem rust resistance derived from 'waldron' wheat.	the chromosomal locations of genes for resistance to stem rust (puccinia graminis pers.: pers. f. sp. tritici eriks. & e. henn.) in the wheat (triticum aestivum l.) cultivar 'waldron' (wdr) were determined by monosomic analyses. wheat lines wdr-b1, -c2, -e4, and -f1,which have single genes for resistance to stem rust derived previously from wdr sel. 'little club', were crossed onto a complete set of 21 'chinese spring' monosomics. the f2 and backcross-f1 (bc1f1) seedlings from each of the 84 cro ...	1995	24173078
assessment of the type and degree of restriction fragment length polymorphism (rflp) in diploid species of the genus triticum.	the a genome of the triticeae is carried by three diploid species and subspecies of the genus triticum: t. monococcum ssp. monococcum, t. monococcum ssp. boeoticum, and t. urartu, the a-genome donor of bread wheat. these species carry many genes of agronomic interest, including disease resistances, and may also be used for the genetic mapping of the a genome. the aim of this study was to evaluate the variability present in a sample of 25 accessions representative of this group using rflp markers ...	1995	24173063
biochemical and molecular diagnostics of thinopyrum bessarabicum chromosomes in triticum aestivum germ plasm.	thinopyrum bessarabicum (2n=2x=14, jj) is a self-fertile salt-tolerant grass species, and its hybridization with triticum aestivum to achieve the transfer of this attributes has been promoted. for the detection of alien introgression, development of diagnostic markers of th. bessarabicum chromosomes in the wheat background has emerged as an important aspect in our intergeneric hybridization program. six proteins/isozymes-high-molecular-weight glutenins, superoxide dismutase, grain esterase, β-am ...	1995	24173049
meiosis and fertility of f1 hybrids between hexaploid bread wheat and decaploid tall wheatgrass (thinopyrum ponticum).	as the first step in the transfer of barely yellow dwarf virus resistance and salt tolerance from decaploid tall wheatgrass (thinopyrum ponticum) into hexaploid bread wheat (triticum aestivum l.), octoploid intergeneric hybrids (2n = 8x = 56) were synthesized by crossing the tall wheatgrass cultivar 'alkar' with wheat cvs. 'fukuhokomugi' ('fuko') and 'chinese spring'. ('fuko' x 'alkar') f1 hybrids were studied in detail. the f1 hybrids were perennial and generally resembled the male wheatgrass p ...	1995	24172931
high crossability of wild barley (hordeum spontaneum c. koch) with bread wheat and the differential elimination of barley chromosomes in the hybrids.	four bread wheat (triticum aestivum l.) cultivars, 'aobakomugi', 'chinese spring', 'norin 61' and 'shinchunaga', were pollinated with five barley lines/cultivars consisting of three cultivated barley (hordeum vulgare l.) lines, 'betzes', 'kinai 5' and ohl089, and two wild barley (hordeum spontaneum c. koch) lines, ouh602 and ouh324. crossability, expressed as the percentage of embryo formation, varied from 0 to 55.4% among the cross combinations. the two wild barley lines generally had a higher ...	1995	24170047
development and molecular cytogenetic analysis of wheat-haynaldia villosa 6vs/6al translocation lines specifying resistance to powdery mildew.	several triticum aestivum l.-haynaldia villosa disomic 6vs/6al translocation lines with powdery mildew resistance were developed from the hybridization between common wheat cultivar yangmai 5 and alien substitution line 6v(6a). mitotic and meiotic c-banding analysis, aneuploid analysis with double ditelosomic stocks, in situ hybridization, as well as the phenotypic assessment of powdery mildew resistance, were used to characterize these lines. the same translocated chromosome, with breakpoints n ...	1995	24170007
detection of genetic diversity in closely related bread wheat using microsatellite markers.	wheat microsatellites (wms) were used to estimate the extent of genetic diversity among 40 wheat cultivars and lines, including mainly european elite material. the 23 wms used were located on 15 different chromosomes, and revealed a total of 142 alleles. the number of alleles ranged from 3 to 16, with an average of 6.2 alleles per wms. the average dinucleotide repeat number ranged from 13 to 41. the correlation coefficient between the number of alleles and the average number of repeats was only ...	1995	24169989
cytologically based physical maps of the group 3 chromosomes of wheat.	cytologically based physical maps for the group 3 chromosomes of wheat were constructed by mapping 25 triticum aestivum deletion lines with 29 t. tauschii and t. aestivum rflp probes. the deletion lines divide chromosomes 3a, 3b, and 3d into 31 discrete intervals, of which 18 were tagged by marker loci. the comparison of the consensus physical map with a consensus rflp linkage map of the group 3 chromosomes of wheat revealed a fairly even distribution of marker loci on the long arm, and higher r ...	1995	24169916
a comparison of male and female recombination frequency in wheat using rflp maps of homoeologous group 6 and 7 chromosomes.	a novel approach was used to compare male and female recombination rates in wheat. doubled haploid lines were developed from an f1 using two distinct approaches: the anther-culture technique and the hordeum bulbosum system, from which sets of lines were developed from "male" and "female" meioses, respectively. the genotype of the lines was established at rflp and isozyme markers polymorphic on chromosomes of homoeologous groups 6 and 7, and "male" and "female" linkage maps were calculated using ...	1995	24169910
comparison of wheat physical maps with barley linkage maps for group 7 chromosomes.	comparative genetic maps among the triticeae or gramineae provide the possibility for combining the genetics, mapping information and molecular-marker resources between different species. dense genetic linkage maps of wheat and barley, which have a common array of molecular markers, along with deletion-based chromosome maps of triticum aestivum l. will facilitate the construction of an integrated molecular marker-based map for the triticeae. a set of 21 cdna and genomic dna clones, which had pre ...	1995	24169889
characterization of deletions in common wheat induced by an aegilops cylindrica chromosome: detection of multiple chromosome rearrangements.	an aegilops cylindrica chromosome induces terminal deletions of chromosomes in wheat as identified by c-banding. we are constructing high-density physical maps of wheat chromosomes and have detected additional chromosome rearrangements. among 63 lines with chromosomal subarm deletions in group 7 chromosomes, 7 lines (11.1%) were shown to harbor additional chromosome rearrangements. two other lines were also omitted from the physical mapping because of the nature of the breakpoint calculations. t ...	1995	24169888
cytologically based physical maps of the group-2 chromosomes of wheat.	we have constructed cytologically based physical maps (cbpms), depicting the chromosomal distribution of rflp markers, of the group-2 chromosomes of common wheat (triticum aestivum l. em thell). twenty-one homozygous deletion lines for 2a, 2b, and 2d were used to allocate rflp loci to 19 deletion-interval regions. a consensus cbpm was colinearily aligned with a consensus genetic map of group-2 chromosomes. the comparison revealed greater frequency of recombination in the distal regions. several ...	1995	24169882
physiological aspects of aluminium tolerance associated with the long arm of chromosome 2d of the wheat (triticum aestivum l.) genome.	aluminum (al) uptake in roots of wheat nearisogenic lines having differing tolerances to aluminium toxicity was studied using roots and root segments immersed in a nutrient solution at a controlled ph and temperature. at low al concentrations a mechanism preventing root tips from accumulating too much al was observed in an al-tolerant isoline and a 'bh1146' euploid. this mechanism was more efficient when divalent cations of calcium or magnesium were present in the nutrient medium. al accumulatio ...	1995	24169843
structural evolution of wheat chromosomes 4a, 5a, and 7b and its impact on recombination.	the construction of comparative genetic maps of chromosomes 4a(m) and 5a(m) of triticum monococcum and chromosomes of homoeologous groups 4, 5 and 7 of t. aestivum has provided insight into the evolution of these chromosomes. the structures of chromosomes 4a, 5a and 7b of modern-day hexaploid bread wheat can be explained by a 4al/5al translocation that occurred at the diploid level and is present both in t. monococcum and t. aestivum. three further rearrangements, a 4al/7bs translocation, a peri ...	1995	24169776
standard karyotype of triticum searsii and its relationship with other s-genome species and common wheat.	c-banding polymorphism was analyzed in 14 accessions of triticum searsii from israel, and a generalized idiogram of the species was established. one accession was homozygous for whole arm translocations t1s(s)s·4s(s)s and t1s(s)l·4s(s)l. c-banding analysis was also used to identify 7 t. aestivum cv 'chinese spring'-t. searsii disomic chromosome addition lines, 14 ditelosomic chromosome addition lines, 21 disomic whole chromosome, and 31 ditelosomic chromosome substitution lines. the identity of ...	1995	24169771
the effect of fluorine atom on the synthesis and composition of gametocidal ethyl oxanilates.	three derivatives of ethyl oxanilate were synthesized in order to test their application as gametocides on the hermaphrodite plants like common wheat (triticum aestivum l.). a substituent at para position (f, br, cn) of aniline defined its reactivity towards diethyl oxalate 2. classical reaction in toluene was not selective and amidation occurred also at the second carbonyl groups of 2. alternative synthesis under solvent-free conditions with application of low pressure for removal of etoh provi ...	2013	24169710
geographical distribution of genes for resistance to formae speciales of erysiphe graminis in common wheat.	the geographical distribution of pm10, pm11, pm14, and pm15 wheat genes for resistance to inappropriate formae speciales of erysiphe graminis was investigated using gene-for-gene relationships. pm10 and pm15 were very common among many indigenous accessions of common wheat collected from various areas in the world. the diversity of genotypes, which consisted of allelic combination at those loci, was high near the center of origin of common wheat and decreased with increasing distance from the ce ...	1995	24169671
the interactive effects of transgenically overexpressed 1ax1 with various hmw-gs combinations on dough quality by introgression of exogenous subunits into an elite chinese wheat variety.	seed storage proteins in wheat endosperm, particularly high-molecular-weight glutenin subunits (hmw-gs), are primary determinants of dough properties, and affect both end-use quality and grain utilization of wheat (triticum aestivum l). in order to investigate the interactive effects between the transgenically overexpressed 1ax1 subunit with different hmw-gs on dough quality traits, we developed a set of 8 introgression lines (ils) overexpressing the transgenic hmw-glutenin subunit 1ax1 by intro ...	2013	24167625
molecular characterization of the fate of transgenes in transformed wheat (triticum aestivum l.).	molecular analysis of the transgenes bar and gus was carried out over successive generations in six independent transgenic lines of wheat, until the plants attained homozygosity. data on expression and integration of the transgenes is presented. five of the lines were found to be stably transformed, duly transferring the transgenes to the next generation. the copy number of the transgenes varied from one to five in the different lines. one line was unstable, first losing expression of and then e ...	1996	24166632
wheat phylogeny determined by rflp analysis of nuclear dna. 3. intra- and interspecific variations of five aegilops sitopsis species.	the level of intra- and interspecific variations on nuclear dna in five aegilops species of the sitopsis section were investigated using restriction fragment length polymorphism (rflp) analysis. a total of 18 accessions, i.e. 7 of ae. speltoides, 3 of ae. longissima, 2 of ae. searsii, 3 of ae. sharonensis and 3 of ae. bicornis, were used. one accession each of triticum aestivum, t. durum, t. urartu and ae. squarrosa was included as reference material. five enzymes and 20 probes were used. among ...	1996	24166619
genetic analysis of anther culture response in wheat carrying alien translocations.	a bread wheat cultivar, 'saratovskaya 29', (s29), its nearly isogenic lines carrying alien translocations [lr9 from aegilops umbellulata (eg29) and (lr19) from agropyron elongatum (ps29)] and two f1 hybrids between three nearly isogenic lines of s29 that differed by the lr19+rht1,pro1+pro2 and ppd1+ppd2 gene complexes, namely the s29 (lr19+rht1)/s29 (ppd1+ppd2) f1 and the s29 (pro1+pro2)/s29 (lr19+rht1) f1 were studied for their culture response with the following results. (1) translocations wit ...	1996	24166404
isolation and identification of triticum aestivum l. em. thell. cv chinese spring-t. peregrinum hackel disomic chromosome addition lines.	analyses of rflps, isozymes, morphological markers and chromosome pairing were used to isolate 12 triticum aestivum cv chinese spring (genomes a, b, and d)-t. peregrinum (genomes s(v) and u(v)) disomic chromosome addition lines. the evidence obtained indicates that each of the 12 lines contains an intact pair of t. peregrinum chromosomes. one monosomic addition line, believed to contain an intact 6s(v) chromosome, was also isolated. a cs-7u(v) chromosome addition line was not obtained. syntenic ...	1996	24166328
rflp-based maps of the homoeologous group-6 chromosomes of wheat and their application in the tagging of pm12, a powdery mildew resistance gene transferred from aegilops speltoides to wheat.	genetic maps of the homoeologous group-6 chromosomes of bread wheat, triticum aestivum, have been constructed spanning 103 cm on 6a, 90 cm on 6b and 124 cm on 6d. these maps were transferred to a chinese spring (cs) x line #31 cross to locate a dominant powdery mildew resistance gene, pm12, introgressed into line #31 from aegilops speltoides. pm12 was shown to lie on the short arm of translocation chromosome 6bs-6ss.6sl in line #31, but could not be mapped more precisely due to the lack of recom ...	1996	24166323
mapping of the k(+)/na (+) discrimination locus kna1 in wheat.	in saline environments, bread wheat, triticum aestivum l. (genomes aabbdd), accumulates less na(+) and more k(+) in expanding and young leaves than durum wheat, t. turgidum l. (genomes aabb). higher k(+)/na(+) ratios in leaves of bread wheat correlate with its higher salt tolerance. chromosome 4d from bread wheat was shown in previous work to play an important role in the control of this trait and was recombined with chromosome 4b in the absence of the ph1 locus. a population of plants disomic f ...	1996	24166270
genetic characterization of storage proteins in a set of f1-derived haploid lines in bread wheat.	wheat storage proteins were evaluated by sds-page in a population of 206 doubled haploid (dh) lines, produced from a cross between bread wheat cvs chinese spring (cs) and courtot (ct). the analysis of gliadins and high- and low-molecular-weight glutenins gave rise to 11 protein markers between parental varieties. among these, one each was encoded at the glu-a1, gli-a1, gli-a2, gli-a5, glu-b3, gli-b1 and gli-d1 loci and four were encoded at the glu-d3 locus. only the gli-a2 marker showed a distor ...	1996	24166255
plant regeneration from immature embryos of 48 elite cimmyt bread wheats.	forty-eight bread wheat (triticum aestivum l.) released cultivars and elite advanced lines were evaluated for their ability to produce embryogenic callus using three different media. basal n6 medium supplemented with dicamba (e1), ms medium containing 2,4-d (e3) or ms medium containing 2,4-d plus different amino acids (e5) were used for callus initiation and maintenance. plant regeneration was achieved on basal ms medium with indole-3-acetic acid (iaa) and 6-benzylamino purine (bap) and rooting ...	1996	24166163
identification of haynaldia villosa chromosomes added to wheat using a sequential c-banding and genomic in situ hybridization technique.	genomic in situ hybridization (gish) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. we integrated c-banding with gish to identify haynaldia villosa chromosomes in a wheat background. all chromosomes of h. villosa showed c-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each h. villosa chromosome. the seven pairs of h. villosa chromosome ...	1996	24166125
wheat syntenome unveils new evidences of contrasted evolutionary plasticity between paleo- and neoduplicated subgenomes.	bread wheat derives from a grass ancestor structured in seven protochromosomes followed by a paleotetraploidization to reach a 12 chromosomes intermediate and a neohexaploidization (involving subgenomes a, b and d) event that finally shaped the 21 modern chromosomes. insights into wheat syntenome in sequencing conserved orthologous set (cos) genes unravelled differences in genomic structure (such as gene conservation and diversity) and genetical landscape (such as recombination pattern) between ...	2013	24164652