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[pneumonia and bone marrow infection with rhodococcus equi in an aids patient]. 19957623501
cytokine modulation alters pulmonary clearance of rhodococcus equi and development of granulomatous pneumonia.rhodococcus equi, a facultative intracellular bacterium, causes chronic, often fatal granulomatous pneumonia in young horses and in humans with aids. the inability of host alveolar macrophages to kill intracellular r. equi results in the development of granulomas and progressive loss of pulmonary parenchyma. clearance of the organism from the lung requires functional cd4+ t cells. the purpose of this study was to identify the cytokine effector mechanisms that mediate clearance of r. equi from th ...19957622227
pseudomonas aeruginosa aliphatic amidase is related to the nitrilase/cyanide hydratase enzyme family and cys166 is predicted to be the active site nucleophile of the catalytic mechanism.a database search indicated homology between some members of the nitrilase/cyanide hydratase family, pseudomonas aeruginosa and rhodococcus erythropolis amidases and several other proteins, some of unknown function. block and profile searches confirmed these relationships and showed that four regions of the p. aeruginosa amidase had significant homology with corresponding regions of nitrilases. a phylogenetic tree placed the p. aeruginosa and r. erythropolis amidases in a group with nitrilases b ...19957607322
case reports: pericarditis and lymphadenitis due to rhodococcus equi.most patients with rhodococcus equi infection are immunocompromised by either hiv infection, malignancy, or medication. diagnosis is frequently missed or delayed because the organisms, resembling diphtheroids on smears, may be regarded as contaminants. their clinical, pathologic, histochemical, and microbiologic resemblance to mycobacteria can result in misdiagnosis. two cases were seen recently in our institution. r. equi pericarditis developed in a 29-year-old woman with failed renal transplan ...19957604837
identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with aids.rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. however, little is known about the characteristics of r. equi isolates from humans. this study characterized the plasmid content, expression of a virulence-associated antigen, and mouse virulence of 19 r. equi isolates from patients with and without aids. ecori digestion patterns and southern, western, and virulence analyses of these isolates with cryptic plasmids allowed definition of a new ...19957594668
cloning and expression of the s-triazine hydrolase gene (trza) from rhodococcus corallinus and development of rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine.we used degenerate oligodeoxyribonucleotides derived from the n-terminal sequence of the s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r in an amplification reaction to isolate a dna segment containing a 57-bp fragment from the trza gene. by using the nucleotide sequence of this fragment, a nondegenerate oligodeoxyribonucleotide was synthesized and used to screen a genomic library of r. corallinus dna for fragments containing trza. a 5.3-kb psti fragment containing trza was cloned ...19957592318
sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5.the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ...19957590299
antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens).the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ...19957587049
the first characterization of a eubacterial proteasome: the 20s complex of rhodococcus.the 26s proteasome is the central protease of the ubiquitin-dependent pathway of protein degradation. the proteolytic core of the complex is formed by the 20s proteasome, a cylinder-shaped particle that in archaebacteria contains two different subunits (alpha and beta) and in eukaryotes contains fourteen different subunits (seven of the alpha-type and seven of the beta-type).19957583123
pathogenesis of rhodococcus equi infection in mice: roles of virulence plasmids and granulomagenic activity of bacteria.virulence of rhocococcus equi atcc 33701 and its plasmid-cured derivative atcc 33701p- was compared in balb/c and c3h/hej mice in terms of bacterial growth kinetics and histological changes in the liver, spleen and lungs, and humoral immune responses. injection with a sublethal dose of 10(6) atcc 33701 in mice resulted in microabscess formation after rapid multiplication in the liver and spleen by day 4, and then the bacteria were gradually eliminated with the formation of granuloma and the prod ...19957581269
dehalogenation of haloalkanes by rhodococcus erythropolis y2. the presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity.rhodococcus erythropolis y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by c3 to c6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by c7 to c16 1-haloalkanes and n-alkanes. the oxygenase-type activity dehalogenated c4 to c18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. the halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes an ...19957579998
an improved escherichia coli-rhodococcus shuttle vector and plasmid transformation in rhodococcus spp. using electroporation.the genetic studies of metabolically diverse rhodococcus spp. have been hampered by the lack of a system of introducing exogenous dna. the authors improved an existing escherichia coli-rhodococcus shuttle vector (pmvs301) by removing much of the dna not needed for replication and adding a multicloning site. this improved vector (pbs305) is 7.9 kb in length. its ability to transform rhodococcus was tested using electroporation parameters optimized for introduction of pmvs301 into rhodococcus. tra ...19957576519
characterization of the rhodococcus sp. ni86/21 gene encoding alcohol: n,n'-dimethyl-4-nitrosoaniline oxidoreductase inducible by atrazine and thiocarbamate herbicides.a protein with a mol.mass of 51,000 (thce) that was induced in rhodococcus sp. ni86/21 during assimilation of thiocarbamate herbicides, atrazine, ethanol, propanol, glycerol, propionaldehyde or ethanolamine was identified by two-dimensional electrophoresis. the thce gene was cloned and sequenced. the deduced amino acid sequence revealed thce as a member of group iii alcohol dehydrogenases. thce displayed strong homology with sequenced subunit fragments of the homodecameric n,n'-dimethyl-4-nitros ...19957575099
multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the gram-positive polychlorinated biphenyl-degrading bacterium rhodococcus erythropolis ta421, isolated from a termite ecosystem.rhodococcus erythropolis ta421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. genetic and biochemical analyses of the pcb catabolic pathway of this organism revealed that there are four different bphc genes (bphc1, bphc2, bphc3, and bphc4) which encode 2,3-dihydroxybiphenyl dioxygenases. as determined by southern hybridization, none of the bphc genes exhibits homology to any other bphc gene. bphc1, bphc2, and bphc4 encode en ...19957574595
sequence and molecular characterization of a dna region encoding the dibenzothiophene desulfurization operon of rhodococcus sp. strain igts8.dibenzothiophene (dbt), a model compound for sulfur-containing organic molecules found in fossil fuels, can be desulfurized to 2-hydroxybiphenyl (2-hbp) by rhodococcus sp. strain igts8. complementation of a desulfurization (dsz) mutant provided the genes from rhodococcus sp. strain igts8 responsible for desulfurization. a 6.7-kb taqi fragment cloned in escherichia coli-rhodococcus shuttle vector prr-6 was found to both complement this mutation and confer desulfurization to rhodococcus fascians, ...19957574582
the sulphydryl-activated cytolysin and a sphingomyelinase c are the major membrane-damaging factors involved in cooperative (camp-like) haemolysis of listeria spp.the negative mutant approach was used in this study to identify listerial cytolytic factors involved in cooperative haemolysis (camp-like phenomenon) with staphylococcus aureus and rhodococcus equi. a listeria monocytogenes non-haemolytic mutant specifically impaired in listeriolysin o (llo) production gave no camp reaction with s. aureus, and was virtually camp-negative with r. equi, indicating that the listerial sulphydryl-activated toxin played a major role in cooperative haemolysis. this was ...19957569324
plasmid prtl1 controlling 1-chloroalkane degradation by rhodococcus rhodochrous ncimb13064.rhodococcus rhodochrous ncimb13064 can dehalogenate and use a wide range of 1-haloalkanes as sole carbon and energy source. the 1-chloroalkane degradation phenotype may be lost by cells spontaneously or after treatment with mitomycin c. two laboratory derivatives of the original strain exhibited differing degrees of stability of the chloroalkane degradation marker. plasmids of approximately 100 kbp (prtl1) and 80 kbp (prtl2) have been found in r. rhodochrous ncimb13064. prtl1 was shown to be car ...19957568468
activation of protein kinase c by mycobacterial cord factor, trehalose 6-monomycolate, resulting in tumor necrosis factor-alpha release in mouse lung tissues.cord factors are mycoloyl glycolipids in cell walls of bacteria belonging to actinomycetales, such as mycobacterium, nocardia and rhodococcus. they induce granuloma formation in the lung and interstitial pneumonitis, associated with production of macrophage-derived cytokines. we studied how cord factors induce biological activities in the cells. cord factors isolated from m. tuberculosis, trehalose 6-monomycolate (mtmm) and trehalose 6,6'-dimycolate (mtdm), enhanced protein kinase c (pkc) activa ...19957559098
quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from comamonas testosteroni 63. the first two enzymes in quinoline and 3-methylquinoline degradation.the enzymes catalysing the first two steps of quinoline and 3-methylquinoline degradation by comamonas testosteroni 63 were investigated. quinoline 2-oxidoreductase, which catalyses the hydroxylation of (3-methyl-)quinoline to (3-methyl-)2-oxo-1,2-dihydroquinoline, was purified to apparent homogeneity. the native enzyme, with a molecular mass of 360 kda, is composed of three non-identical subunits (87, 32, and 22 kda), occurring in a ratio of 1.16:1:0.83. containing fad, molybdenum, iron, and ac ...19957556204
assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16s rrna genes of corynebacteria.comparative 16s rrna gene sequencing was used to assign four isolates of spontaneous urinary calculus in young laboratory rats. the phylogenetic relationships among the rat isolates and selected species of corynebacteria were also inferred. based on the homology and evolutionary distance analysis, the 16s rrna genes of the rat isolates were almost identical with that of corynebacterium renale atcc 19412. also the results of the phylogenetic analysis showed a close relationship among the isolates ...19957548409
comparison of mycobacterium 23s rrna sequences by high-temperature reverse transcription and pcr.we describe a modified rrna sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus mycobacterium. we combined the sensitivity of the reverse transcriptase pcr for amplifying nanogram amounts of template rrna material with the elevated extension temperatures used for the thermostable dna polymerase from thermus thermophilus. a 70 degrees c reverse transcription extension step permitted improved read-through of highly structured r ...19957547304
phylogeny of the genus corynebacterium deduced from analyses of small-subunit ribosomal dna sequences.we determined almost complete small-subunit ribosomal dna sequences of 50 reference strains belonging to the genera corynebacterium, rhodococcus, and gordona and compared these sequences with previously published sequences. three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. the results of comparative phylogenetic analyses confirmed that the ge ...19957547293
phylogenetic analysis of the genus corynebacterium based on 16s rrna gene sequences.the 16s rrna gene sequences of 30 strains representing 23 validated corynebacterium species and 7 currently non-valid corynebacterium species were determined. these sequences were aligned with the sequences of other corynebacterium species and related actinomycete taxa. a comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus corynebacterium. turicella otitidis and the amycolate species corynebacterium amycolatum were located at t ...19957547291
phylogenetic analysis of mycolic acid-containing wall-chemotype iv actinomycetes and allied taxa by partial sequencing of ribosomal protein at-l30.the phylogenetic relationships among 30 mycolic acid-containing wall chemotype iv actinomycete strains and 12 strains belonging to allied taxa were examined by determining the amino acid sequences of the ribosomal at-l30 proteins of these organisms. sequencing 20 n-terminal amino acids of at-l30 preparations revealed that the members of the genera containing mycolic acid-containing actinomycetes form two clusters; the first cluster contains the genera nocardia, rhodococcus, gordona, and tsukamur ...19957547284
a phylogeny of the genus nocardia deduced from the analysis of small-subunit ribosomal dna sequences, including transfer of nocardia amarae to the genus gordona as gordona amarae comb. nov.according to phylogenetic analyses of nearly complete small-subunit ribosomal dna sequences, the genus nocardia should not comprise the two species nocardia petroleophila and nocardia amarae. n. amarae should be reassigned to the genus gordona as gordona amarae. all of the other nocardia species form a monophyletic unit, closely related to species of the genus rhodococcus. it is proposed to revive the name 'cmn' to comprise the genera corynebacterium, tsukamurella, mycobacterium, gordona, rhodoc ...19947545965
a phylogenetic analysis of the genus nocardia with 16s rrna gene sequences.partial sequences of the 16s rrna genes of the type strains of nine species of the genus nocardia were determined following the isolation and cloning of the amplified genes. these sequences were aligned with the sequences of representatives of the genera corynebacterium, gordona, mycobacterium, rhodococcus, and tsukamurella, and phylogenetic trees were inferred by using the fitch-margoliash and neighbor-joining methods. the genus nocardia formed a distinct clade that was most closely associated ...19957537058
phylogenetic evidence for transfer of pentachlorophenol-mineralizing rhodococcus chlorophenolicus pcp-i(t) to the genus mycobacterium.we determined the nucleotide sequence of a 16s rrna gene of rhodococcus chlorophenolicus pcp-i(t) (= dsm 43826t) (t = type strain). sequence comparisons revealed that there was a close relationship between strain pcp-i(t) and strains belonging to the genus mycobacterium. the sequence data were used to construct a phylogenetic tree, which showed that mycobacterium chubuense is the closest relative of strain pcp-i(t). we propose that strain pcp-i(t) should be transferred to the genus mycobacterium ...19947520738
rhodococcus equi pneumonia and pulmonary malakoplakia in acquired immunodeficiency syndrome. pathologic features.pulmonary infection with rhodococcus equi is rare, and most cases are seen in immunocompromised patients, particularly those with acquired immunodeficiency syndrome. we describe the pathologic features in four cases of culture-positive r equi pneumonia occurring in patients infected with human immunodeficiency virus. all four patients had a solitary cavitary pulmonary mass that was resected (n = 3) or had undergone biopsy (n = 1). pathologically, all specimens revealed sheets of histiocytes with ...19947517660
rhodococcus equi-associated necrotizing lymphadenitis in a llama.a case of rhodococcus equi-associated necrotizing lymphadenitis in a 2-year-old male llama is described. caseous necrosis, resembling macroscopically that seen in ovine caseous lymphadenitis, was observed diffusely in the tracheobronchial and mediastinal lymph nodes, and in an extensive lesion in the lungs. necrosis was present to a lesser extent in the spleen and hepatic and gastric lymph nodes. numerous bacteria-laden macrophages were present around the necrotic areas. the findings suggest tha ...19957490342
reclassification of mycobacterium phlei pn-bb as rhodococcus bronchialis pn-bb.mycobacterium phlei pn-bb, induced in the parental strain pn by gamma-radiation and ethyl methanesulfonate, should be reclassified as rhodococcus bronchialis pn-bb. the reclassification is based on the results of lipid analysis, dna-dna hybridization and several biochemical tests, performed in comparison with the parental strain pn, earlier reclassified as r. bronchialis, and r, bronchialis (n654).19807469254
septic arthritis and osteomyelitis caused by an organism of the genus rhodococcus.we describe a previously healthy, immunologically normal young girl who presented painless swelling of fingers, a toe, and one knee. roentgenograms were consistent with osteomyelitis of the phalanges and knee effusion. rice bodies (corpora oryzoidea) were identified in viscous fluid obtained from the knee during arthroscopy. culture of this fluid grew an organism initially believed to be a member of the genus nocardia but which was later presumptively identified as a member of the genus rhodococ ...19817462414
response of developing branched bacteria to adverse environments. ii. micromorphological effects of lysozyme on some aerobic actinomycetes.an early symptom of lysozyme treatment of developing actinomycete microcolonies is hyphal tip swelling, illustrating the plasticity of this region in relation to filament extension. the reaction is not limited to true mycelia of streptomyces, streptoverticillium and rhodococcus but is also characteristic of unbranched filaments of actinomycetes exposed during their elongation stage. on the other hand rods whose extension has ceased and which are undergoing fragmentation do not show any localized ...19807424249
[difference of uptake of 2,6-diamino (g-3h) pimelic acid dihydrochloride among mycobacterium, rhodococcus, and nocardia (author's transl)]. 19807373966
occurrence of rhodococcus coprophilus and associated actinomycetes in feces, sewage, and freshwater.freshwater, sewage, and fecal samples from various sources were examined for rhodococcus coprophilus, associated actinomycetes, escherichia coli, and fecal streptococci. rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater polluted with animal fecal wastes. it was not isolated from samples of human feces. the ratio of r. coprophilus total actinomycetes was higher in feces from cattle, sheep, ducks, a ...19817316510
[thin-layer chromatography after incubation with 35s-methionine.--meaning of petroleum ether-soluble fraction as aid to identification of mycobacterium, rhodococcus, and nocardia--(author's transl)]. 19807289348
porcine abortions associated with fungi, actinomycetes, and rhodococcus sp.history, lesions, and results of microbiologic examinations are given for four porcine abortions associated with fungi and one each associated with actinomadura madurae, an aerobic actinomycete, and rhodococcus sp. the fungi were gliomastix sp., petriellidium boydii, aspergillus fumigatus, and exophiala jeanselmei. parvovirus, isolated from one fetus, was the only other infectious agent identified. the presence of fungi, actinomadura madurae, and rhodococcus sp. in fetal and placental lesions in ...19817281459
rhodococcus (corynebacterium) equi: a possible cause of reactions to the complement fixation test for johne's disease of cattle. 19817271613
the treatment of pneumonia in foals caused by rhodococcus (corynebacterium) equi. 19817259664
in-vitro susceptibility of nocardia and rhodococcus to fourteen beta-lactam antibiotics. 19827161233
lung infection caused by gordona aurantiaca (rhodococcus aurantiacus).we report that gordona aurantiaca (rhodococcus aurantiacus) caused a lung infection in humans. the case of a 50-year-old male farmer is shown. this is the first report of infection by this organism.19827153308
numerical and chemical classification of nocardia amarae.twenty-one strains of nocardia amarae and marker cultures of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were subjected to numerical phenetic analyses using 92 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using the unweighted average linkage algorithm. neither cluster nor aggregate cluster composition was markedly affected by the coefficient used or by test error, estimated at 1 ...19827119738
[key rhodococcus enzymes in the catabolism of aromatic compounds].the enzyme apparatus involved in the catabolism of aromatic compounds in rhodococci is characterized by the presence of pyrocatechase and protocatechoate-3,4-dioxygenase as principal enzymes cleaving the aromatic cycle. metapyrocatechase was found in about 30% of the rhodococci. all the enzymes are inducible. the inductor of pyrocatechase seems to be cyc-cys-muconate, and that of protocatechase appears to be 3-oxoadipate. the metapyrocatechase of rhodococci, in contrast to that of pseudomonas, i ...19827087811
a survey of the structures of mycolic acids in corynebacterium and related taxa.acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ...19827086391
capsular serotypes of rhodococcus equi.one hundred strains of rhodococcus equi from various animal species and sources in australia were examined for capsular serotype. eighty-four of the strains fell into the existing 7 serotypes, and just under half of the strains belonged to serotype 1. isolates from the intestines and faeces of horses, cattle, pigs and other species, and from soil, were found to belong to the same serotypes as those recovered from the lungs of foals with r. equi pneumonia. there was no clear relationships between ...19827082241
a survey of mycobacteriosis of feral pigs in the northern territory.seven hundred and fifty-one feral pigs from the subcoastal plains of the northern territory were examined. the sample population consisted of 52.4% females and 47.6% males. they ranged in age from newborn piglets to mature animals of over 72 months. of the pigs examined 47.7% had macroscopic abscesses and of these 80.2% were probably caused by mycobacteria. tissues from 193 pigs were examined bacteriologically and 93 strains of mycobacteria were isolated. these were typed as m. bovis (37 strains ...19817041875
differentiation between the genera mycobacterium, rhodococcus and nocardia by susceptibility to 5-fluorouracil.a test for susceptibility to 5-fluorouracil was useful for differentiating between species of rapidly growing mycobacteria, and for differentiating the genus rhodococcus from the genus nocardia. the majority of rhodococci tested were susceptible to 5-fluorouracil (20 micrograms ml-1), whereas the majority of nocardiae tested were resistant to it. strains of nocardia asteroides sensu stricto could be divided into two subgroups by their reaction to 5-fluorouracil.19817038035
the distribution of mycobacterium bovis in queensland cattle herds with observations on the laboratory diagnosis of tuberculosis.of 1,791 tissues submitted from 1975 to 1978 for histological examination for tuberculosis, 78.5% were classified as tuberculosis, 8.1% as club-forming granulomas, 3.3% as hydatid cysts, 3.4% as other granulomas and 2.4% as squamous cell carcinomas. cultural examination of 2,682 tissues for mycobacteria yielded mycobacterium bovis from 42.88%, other mycobacteria from 7.64% and 0.89% rhodococcus (corynebacterium) equi. of 1,478 tissues on which both examinations were performed, culture and histol ...19807018487
comparison of three techniques for isolation of rhodococcus (corynebacterium) equi from contaminated sources.inoculation of a liquid medium comprised of trypticase soy broth (bbl microbiology systems), cycloheximide, nalidixic acid, penicillin, and potassium tellurite and subcultured onto m3 medium plus potassium tellurite was highly successful for the isolation of rhodococcus (corynebacterium) equi from soil.19817007424
numerical classification of some named strains of nocardia asteroides and related isolates from soil.one hundred and forty-nine strains of nocardiae, freshly isolated from soil samples obtained from a number of countries with either tropical or temperate climates, and from rubber pipe seals, were compared with appropriate marker cultures in a numerical phenetic study using 156 unit characters. marker strains were chosen to represent the nocardia asteroides complex, other nocardia species and related taxa in an effort both to classify the new soil isolates and, possibly, clarify the structure of ...19807003057
immunity to and immunotherapy for rhodococcus equi.immune responses to rhodococcus equi were assayed in mares and foals on 7 studs in south-eastern australia using skin test reactivity to the intradermal injection of culture filtrate and an indirect fluorescent antibody test. the prevalence of positive skin-test reactions did not differ between studs with a history of r. equi disease and those without but there were more mares with high antibody titres on studs with a disease history. a leucocyte extract prepared from mares that were skin-test p ...19826962890
is rhodococcus equi a soil organism?a total of 189 isolates of rhodococcus equi and related organisms and 16 marker strains representing the genera rhodococcus and corynebacterium were screened for 160 unit characters in a numerical taxonomic study. analysis of the data indicated that r. equi forms a relatively homogeneous cluster distinctly separated from the recognized species of rhodococcus and corynebacterium (sensu stricto). other members of the genus rhodococcus are soil organisms and r. equi appears to fit into the genus on ...19826962888
numerical classification of rhodococcus equi and related actinomycetes. 19826819287
fatty acid composition of some mycolic acid-containing coryneform bacteria.the fatty acid profiles of 74 strains of mycolic acid-containing coryneform bacteria were examined by gas-liquid chromatography. all of the strains contained major amounts of straight-chain and monounsaturated fatty acids although some also possessed substantial amounts of 10-methyloctadecanoic acid. iso- and anteiso-branched acids were not present. five distinct fatty acid patterns were evident: (i) corynebacterium diphtheriae, c. pseudotuberculosis and 'c. ulcerans' strains contained major amo ...19826818327
hemolytic properties of exosubstance of serovar 5 listeria monocytogenes compared with beta toxin of staphylococcus aureus.hemolytic exosubstance of serovar 5 listeria monocytogenes, tested in native and pre-purified forms, produced identical interactions with exosubstances of certain bacterial species to those of staphylococcus aureus beta toxin on agars with ovine, rabbit, human and equine erythrocytes. in the same way like the staphylococcal beta toxin, it enhanced hemolysis of other l. monocytogenes serovars, though only slightly and after a longer time of incubation. with the hemolysin of corynebacterium pyogen ...19826812312
numerical classification of some rhodococci, corynebacteria and related organisms.nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...19826811696
routine test for in vitro differentiation of pathogenic and apathogenic listeria monocytogenes strains.the exosubstance of rhodococcus equi in a prepurified form strongly enhanced the hemolytic effect of certain strains of listeria monocytogenes. the strains which produced positive synergic hemolysis with this exosubstance were also pathogenic for guinea pigs and white mice. the other strains, which remained nonhemolytic in the presence of the r. equi. exosubstance, were apathogenic for those animals. a routine test was devised for the in vitro determination of the pathogenicity of l. monocytogen ...19826804488
test for susceptibility to mitomycin c as aids for differentiating the genus rhodococcus from the genus nocardia, and for differentiating mycobacterium fortuitum and mycobacterium chelonei from other rapidly growing mycobacteria. 19816799752
[development cycles of coryneform and nocardia-like bacteria].the growth cycles and the types of cell separation were studied in a microchamber with the collection strains of brevibacterium ammoniagenes attc 6871, b. helvolum atcc 19239, b. linens ccm 47 and atcc 9174, b. maris vkm b-464 and b. stationis atcc 14403, as well as with the strains of the genus rhodococcus isolated from soils, viz. r. maris sp. nov. imb 283 and r. luteus sp. nov. imb 385. according to the increasing complexity of cellular morphological transformation in the life cycle, the orga ...19806782438
numerical analysis of the taxonomy of nocardiae and rhodococci.the genera nocardia and rhodococcus were clearly differentiated in the present study. eleven characteristics were shown to be useful for differentiation between these two genera. nocardia asteroides sunsu stricto previously defined by tsukamura was divided into two taxa. one contained the type strain and was considered to retain the name nocardia asteroides in a new sense. another was named in the present study as nocardia nova sp. nov. tsukamura. the type strain of this species is atcc 33726. t ...19826763136
[rhodococcus isolated from a gluteal abscess].the pathogenic role of the genus rhodococcus as an infectious agent in human beings is very small. its taxonomic allocation may be difficult since there is no systematic description of this bacterial group. this paper summarizes the international literature on the microbiologic diagnosis of rhodococcus and the small number of known cases of rhodococcus infections. a description is given of the first case of a post-injection gluteal abscess caused by a rhodococcus sp.19826759413
isolation and characterization of an intracellular serine protease from rhodococcus erythropolis. 19826758705
ecology of rhodococcus equi.a selective broth enrichment technique was used to study the distribution of rhodococcus equi in soil and grazing animals. rhodococcus equi was isolated from 54% of soils examined and from the gut contents, rectal faeces and dung of all grazing herbivorous species examined. rhodococcus equi was not isolated from the faeces or dung of penned animals which did not have access to grazing. the isolation rate from dung was much higher than from other samples and this was found to be due to the abilit ...19846719819
quantitative culture of rhodococcus equi from the feces of horse.the selective isolation of r. equi in nanc medium was made by quantitative culture of the organism in the feces. r. equi was observed in the feces of all the mares and foals investigated. the mean viable count of r. equi organisms in 1 gram of feces was 8.42 x 10(2) in the mares and 7.57 x 10(2) in the foals, and its 95% confidence limits were in a range of 6.48 x 10(2) to 1.09 x 10(3) and 4.19 x 10(2) to 1.37 x 10(3), respectively. this result indicates that r. equi is a member of the normal in ...19836680772
serogrouping of rhodococcus equi.the serological relationships among 27 isolates of rhodococcus equi selected from a total of 1,195 isolates were investigated by cross-agglutination and absorption tests. the presence of capsular material was demonstrated in all the 27 isolates by electron microscopic observation. antisera were prepared by employing formalized antigen of each isolate. in the cross-agglutination test with formalized antigen, 13 antisera reacted with homologous antigens alone, but the remaining 14 antisera reacted ...19836669074
virulence of rhodococcus equi for mice. 19836656069
[circumstances leading to the establishment of genus rhodococcus]. 19836655873
degradation of aniline and monochloroanilines by rhodococcus sp. an 117 and a pseudomonad: a comparative study.two newly isolated aniline-degrading bacterial strains were characterized with regard to their enzyme systems responsible for aniline catabolism. one of them identified as a rhodococcus sp. metabolized aniline exclusively via the beta-ketoadipate pathway by means of inducible enzymes. the aniline-degrading enzyme system of the second isolate, presumably a pseudomonad, was shown to consist of an inducible aniline-converting enzyme and constitutive meta-pathway enzymes. both isolates failed to met ...19836613168
equine humoral immune response to rhodococcus (corynebacterium) equi.an enzyme-linked immunosorbent assay was developed to test equine serum for the presence of antibodies to rhodococcus (corynebacterium) equi. experimental ponies had no detectable antibody to r equi before exposure to the bacterium. after experimental inoculation, animals in groups that received live r equi subcutaneously or intranasally/intratracheally developed high titers to r equi. noninoculated controls remained seronegative. serum was also collected from horses of various ages that were na ...19846524739
equine cell-mediated immune response to rhodococcus (corynebacterium) equi.a lymphocyte blastogenic assay was developed to serve as an in vitro correlate of cell-mediated immunity to rhodococcus (corynebacterium) equi (r equi) in the equine species. lymphocytes obtained from a group of experimental ponies showed no response in cell culture to r equi heat extract or lysozyme extract antigens. ponies were assigned to groups for experimental inoculation. three ponies were inoculated subcutaneously with live r equi, 3 were given live r equi by intranasal and intratracheal ...19846524738
effect of rhodococcus equi on equine polymorphonuclear leukocyte function.a procedure was developed for isolating large numbers of purified polymorphonuclear leukocytes (pmns) from the peripheral blood of horses. equine pmn function was evaluated by three procedures: 1) staphylococcus aureus ingestion, 2) nitroblue tetrazolium reduction, and 3) iodination. four preparations of r. equi were added to polymorphonuclear leukocytes (pmns) in each test system. live bacteria, heat-killed bacteria, the washed pellet from heat-killed bacteria, and the supernatant fluid from he ...19846506452
rhodococcus equi pneumonia in foals.rhodococcus (corynebacterium) equi pneumonia is diagnosed by thoracic auscultation, radiographic and hematologic examination, and transtracheal aspiration. treatment may be unsuccessful because of the organism's tendency to cause pulmonary abscesses. a 2-month-old, depressed, anorectic, febrile quarter horse colt, previously unresponsive to penicillin therapy, had loud, moist breath sounds in the ventral lung fields. chest radiographs revealed pneumonia. based on culture and sensitivity tests on ...19846504018
the fate of methoxychlor in soils and transformation by soil microorganisms.methoxychlor was found to be sufficiently persistent in soil and its residues were present even 18 months after the soil treatment. saprophytes, fungi and actinomyces were unaffected by varying concentrations of methoxychlor, azotobacter however was susceptable. soil strains isolated did not utilize methoxychlor as a sole carbon source except for 9 cultures belonging to the genera bacillus, acinetobacter and rhodococcus which carried out the complete dechlorination, demethylation and splitting o ...19846491174
serotaxonomical analyses of strains referred to nocardia amarae and rhodococcus equi.four strains of nocardia amarae and four strains of rhodococcus equi (earlier designated corynebacterium equi) were analysed by means of the comparative immunodiffusion technique for taxonomical purposes. nineteen reference precipitation systems, representing different species of corynebacterium, mycobacterium, nocardia, rhodococcus, streptomyces, and related taxa were employed. the n. amarae strains differed serologically from the reference strains of nocardia and their assignment to the latter ...19846442818
[free mycolic acids of the cells of coryneform and nocardia-like bacteria].the composition of free mycolic acids was studied in the cells of brevibacterium ammoniagenes atcc 6871, b. flavum 22, b. stationis atcc 14403, corynebacterium divaricatum atcc 14020 and rhodococcus maris imv 195. the acids are a mixture of saturated and unsaturated compounds with the total number of carbon atoms from 32 to 36 and the number of c atoms in the alpha-chain from 10 to 15.19846431238
isonicotinic acid hydrazide induced changes and inhibition in mycolic acid synthesis in nocardia and related taxa.the mycolic acid compositions of nocardia rubra and related bacteria grown in media containing different concentrations of antituberculous isonicotinic acid hydrazide (inh) were determined in detail by gas chromatography-mass spectrometry. on the basis of molecular species composition, average carbon numbers of mycolic acids were calculated. in nocardia rubra, n. lutea and rhodococcus rhodochrous ifo-13161, the ratio of mycolic to non-mycolic fatty acids and the average carbon numbers of mycolic ...19846428369
gas chromatography-mass spectrometry of mycolic acids as a tool in the identification of medically important coryneform bacteria.the mycolic acid derivatives of 11 unidentified pathogenic coryneform bacteria were examined by tlc, glc and glc-mass spectrometry. the resulting mycolic acid profiles of the unidentified isolates were compared with those of type or reference strains of possibly related coryneform species, namely corynebacterium bovis, c. diphtheriae, c. xerosis and rhodococcus equi. it was apparent that most of the unidentified strains showed a distinctive mycolic acid profile, with predominant amounts of relat ...19846427401
cross-reactivity between mycobacterium tuberculosis h37rv and various actinomycetes and related organisms.one hundred and forty-one strains of actinomycetales and related organisms were investigated by immunodiffusion for the presence of antigens which cross-react with the antigens of m. tuberculosis h37rv. the test strains comprised 86 different species names and 20 different genus names; they were mainly environmental organisms isolated from soil, plants, animals and such like. more than 90% of these strains were shown to have one or more antigens in common with the tubercle bacillus, and 77% had ...19836415879
identification key for coryneform bacteria derived by numerical taxonomic studies.six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...19836413644
immunodiffusion analyses of some diphtheroid organisms isolated from patients with leprosy.eight strains of diphtheroid bacteria isolated from patients with leprosy were analyzed by immunodiffusion, using precipitation systems representing various species of corynebacterium, mycobacterium, nocardia, rhodococcus, and related organisms. the analyses showed that six of the eight strains shared several antigens with representatives of these four genera. the largest number of shared precipitinogens was revealed when the corynebacterial precipitation systems were used, thus indicating that ...19836413437
[biochemical differentiation of the "listeria monocytogenes" (sensu lato) genomic groups].camp-tests with staphylococcus aureus and rhodococcus equi and acid production from d-xylose allowed to separate the five dna relatedness groups described for listeria monocytogenes sensu lato; acid production from l-rhamnose and alpha-methyl-d-mannoside are secondary markers.19836405670
thymidine kinase of bacteria: activity of the enzyme in actinomycetes and related organisms.various micro-organisms were studied for their thymidine kinase (adenosine 5'-triphosphate:thymidine 5'-phosphotransferase, ec 2.7.1.21) (tk) activity. the sonicated cell extract of escherichia coli k12 had a tk activity of 35-66 pmol thymidine monophosphate formed min-1 (mg protein)-1. the cell extracts of salmonella typhimurium and klebsiella pneumoniae showed a markedly higher (5- to 11-fold) tk activity. somewhat lower but significant tk activity was detected in the cell extracts of staphylo ...19846381650
rapid microbiochemical identification of corynebacterium diphtheriae and other medically important corynebacteria.a rapid biochemical method based on the fermentation of carbohydrates, the hydrolysis of urea, and the reduction of nitrate was used to identify corynebacterium diphtheriae, c. ulcerans, c. pseudodiphtheriticum, c. haemolyticum, c. pseudotuberculosis, c. pyogenes, c. ovis, the centers for disease control jk group, and rhodococcus (corynebacterium) equi. with this procedure identification was confirmed for 133 stock cultures and clinical isolates of corynebacteria. most were identified within 1 h ...19836355166
crossreactivity between mycobacterium leprae and various actinomycetes and related organisms.serological crossreactivity was analyzed between m. leprae and strains of various species of corynebacterium, mycobacterium, nocardia, rhodococcus, streptomyces, and related organisms. m. leprae shares antigens with most of these organisms, and sera from patients with lepromatous leprosy contain antibodies against them. the results demonstrate that m. leprae shares more antigens with the mycobacteria than with strains of the other tested genera, thus supporting the view that the leprosy organism ...19836352526
recurrent skin infection with rhodococcus in an immunosuppressed patient.a renal transplant patient taking prednisone and azathioprine has had repeated episodes of skin infection with the soil saphrophyte rhodococcus. human disease with this organism has not been proved before. although the lesions have always responded to antibiotics, frequent recurrence makes the long-term outlook uncertain.19836350469
investigation of the survival characteristics of rhodococcus coprophilus and certain fecal indicator bacteria.rhodococcus coprophilus and clostridium perfringens survived in fresh water samples held at 5, 20, and 30 degrees c for over 17 weeks, whereas escherichia coli and fecal streptococci disappeared after 5 weeks at all three temperatures. r. coprophilus survived for more than 8 months in sterilized sewage and deionized water at all three temperatures, whereas in normal sewage held at 20 degrees c, the survival time was 12 to 26 weeks. in samples held at 30 degrees c, survival times were shorter, pr ...19836312883
sensitivity to capreomycin and prothionamide in strains of mycobacterium, nocardia, rhodococcus, and related taxa for taxonomical purposes.sensitivity to capreomycin and to prothionamide was analysed for 150 strains belonging to the genera mycobacterium, nocardia, rhodococcus, and related taxa. the analyses showed, e.g., that strains of mycobacterium chelonei and nocardia brasiliensis were more resistant to capreomycin than the other strains tested and that m. farcinogenes differed from m. senegalense concerning sensitivity to this drug. the analysis showed, furthermore, that strains of mycobacterium differ from those of nocardia, ...19836196927
fine structural studies of rhodococcus species.fine structural aspects of rhodococcus rhodochrous and r. equi are described and illustrated by electron micrographs after staining of cells by a variety of electron cytochemical procedures. the cell contents of these actinomycetous bacteria were those of a typical prokaryotic cell and consistent with that observed for other species of the actinomycetales. fixation with either osmium tetroxide or permanganate indicated the presence of an electron opaque substance at the wall exterior of r. rhodo ...19836191182
differentiation between the genera rhodococcus and nocardia and between species of the genus mycobacterium by susceptibility to bleomycin. 19826185624
ribosomal ribonucleic acid similarities in the classification of rhodococcus and related taxa.duplexes were prepared between 14c-labelled rrna from both rhodococcus equi c7 and rhodococcus rhodochrous n54 and dna from 16 actinomycetes representing the genera rhodococcus, mycobacterium, nocardia, saccharopolyspora and streptomyces. the relationships between the organisms were determined by plotting the temperature at which 50% of the duplex was denatured (tm(e)) against the percentage of rrna binding (microgram 14c-labelled rrna duplexed per 100 micrograms filter-bound dna). all of the st ...19806160195
[periodic macromolecule syntheses in synchronized cultures of species belonging to the "rhodococcus" genus ("rhodochrous" group) (author's transl)].the synthesis of total proteins, total rna and dna in exponential synchronous cultures of species belonging to the rhodococcus genus (nocardia restricta and n. canicruria) has been studied by chemical methods and pulsed incorporations of labelled precursors in the acid-insoluble fraction. the replication of dna is discontinuous. syntheses of rna and proteins are periodic: they happen during two main periods during the time necessary for a doubling of the cell mass. a slowdown of these syntheses ...19806155818
further studies on thymidine kinase: distribution pattern of the enzyme in bacteria.various micro-organisms (131 strains of 73 species) were studied for their ability to produce thymidine kinase (tk; ec 2.7.1.21). taking the specific tk activity of escherichia coli k12 [specific activity of sonicated cell extracts 95-194 pmol min-1 (mg protein)-1] as 100%, the test organisms had the following relative specific tk activities. in the gram-positive cocci, staphylococcus aureus (21-84%) showed higher activity than staph. epidermidis (1-20%) and streptococcus (1-7%) except for one s ...19854093763
enzyme-linked immunosorbent assay for diagnosis of corynebacterium (rhodococcus) equi infection in foals.an enzyme-linked immunosorbent assay (elisa) was used to diagnose corynebacterium (rhodococcus) equi infection in foals. in tests done with different antigen-extraction procedures (sodium dodecyl sulfate, sodium deoxycholate, polyoxy-ethylene [9] p-tert-octylphenol, polyoxy-ethylene [9-10] p-tert-octylphenol, sonification, homogenization, and heat treatment at 121 c), tween 20 was a satisfactory reactive antigen. using hyperimmune rabbit sera or infected foal sera, we investigated the specificit ...19854062024
cefadroxil in the horse: pharmacokinetics and in vitro antibacterial activity.sodium cefadroxil was administered as a single intravenous dose (25 mg/kg) to six healthy adult mares. plasma samples were collected over a 24-h period and cefadroxil concentrations were measured by microbiological assay. the pharmacokinetic behavior of the drug was appropriately described in terms of a one-compartment open model. values for the major pharmacokinetic terms were: extrapolated initial plasma concentration = 59.2 +/- 15.0 micrograms/ml; half-life = 46 +/- 20 min; apparent volume of ...19854057345
meningitis caused by gordona aurantiaca (rhodococcus aurantiacus).in a case of hairy cell leukemia, gordona aurantiaca (rhodococcus aurantiacus) was isolated from cerebrospinal fluid as the pathogen responsible for lethal infection of the central nervous system. the pathogen had been isolated previously from one case of pulmonary infection process only.19854044805
the incidence of rhodococcus (corynebacterium) equi in domestic animals and soil. 19854032938
corynebacterium pseudotuberculosis exotoxin: fatal hemolytic anemia induced in gnotobiotic neonatal small ruminants by parenteral administration of preparations containing exotoxin.inoculation of live corynebacterium pseudotuberculosis, culture supernatant, ammonium sulfate-fractionated crude exotoxin, or chromatographically purified exotoxin preparations into gnotobiotic small ruminants (n = 13) caused death of the ruminants within 48 hours. characteristic changes observed in animals living greater than or equal to 2 hours after inoculation included hemorrhage and edema at the site of injection, severe hemolytic anemia and hemoglobinuria, dark red fluid in body cavities, ...19854003896
bacterial interactions in bovine respiratory and reproductive infections.the ability of the aerobic bacterial flora from the normal bovine respiratory and reproductive tracts to enhance or inhibit the growth of pasteurella haemolytica, p. multocida, and haemophilus somnus was tested in vitro. six strains of each of these pathogens were cross streaked with each isolate of bovine normal flora. flora which enhanced the growth of these pathogenic bacteria outnumbered inhibitors four to one. an intermediate number of isolates produced no effect on pathogen growth. most en ...19853998115
susceptibility of equine bacterial isolates to antimicrobial agents.in vitro antimicrobic susceptibility patterns of commonly isolated aerobic gram-positive and gram-negative bacterial pathogens of equine origin were determined, using the agar-plate dilution method. all organisms were recent clinical isolates and included corynebacterium (rhodococcus) equi, corynebacterium pseudotuberculosis, (coagulase positive) staphylococcus sp, streptococcus equi, streptococcus zooepidemicus, actinobacillus sp, enterobacter cloacae, escherichia coli, klebsiella pneumoniae, p ...19853994111
menaquinone composition of mycolic acid-containing actinomycetes and some sporoactinomycetes.the menaquinones of 141 actinomycetes representing the genera caseobacter, mycobacterium, nocardia, rhodococcus and some related taxa lacking mycolic acids were examined by mass spectrometry. the mycolic acid-containing strains were assigned to four groups on the basis of the predominant isoprenologue detected: rhodococcus coprophilus, r. equi, r. erythropolis, r. globerulus, r. rhodnii, r. rhodochrous and r. ruber contained dihydrogenated menaquinones with eight isoprene units; nocardia asteroi ...19853980298
characterization of phages derived from strains of rhodococcus australis and r. equii.rhodococcus australis strain csir-a201 and r. equii strain csir-a655 spontaneously liberated phages a and b, respectively. the phages have different host ranges, but both infect r. rubropertinctus atcc 14352. phage infection of strains resulted in stable lysogeny. the phages have similar morphologies and belong to the family styloviridae. the dna restriction enzyme patterns of the phages differ and do not correspond to those of actinophage phi ec. these temperate phages did not transduce prototr ...19853980185
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