Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the potential of flow cytometry in the study of bacillus cereus. | flow cytometry (fcm) is a rapid method allowing the acquisition of multiparametric data from thousands of individual cells within a sample. as well as measuring the intrinsic light scattering properties of cells, a plethora of fluorescent dyes may be employed to yield information on macromolecule content, surface antigens present or physiological status. despite fcm's indispensability within other fields e.g. immunology, it is underutilized within microbiological research. in this review, a stro ... | 2010 | 19486207 |
| correction of axial chromatic aberrations in confocal raman microspectroscopic measurements of a single microbial spore. | herein we describe a strategy for correcting the longitudinal or axial component of chromatic aberration in confocal raman microspectroscopy. the method is based on measuring a vertical series of confocal raman sections of samples by a high numerical aperture raman microscope. using the known characteristics of the wavelength-dependent focal shift of the optical system, the raman intensities can be corrected to allow the rearrangement of raman data from different focal planes. in the present stu ... | 2009 | 19475143 |
| phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal raman microspectroscopy. | single cells in genetically homogeneous microbial cultures exhibit marked phenotypic heterogeneity that is considered to bolster the fitness of the whole population. heterogeneity on the single-cell level is typically masked in conventional studies of microbial populations, which rely on data averaged across thousands or millions of cells in a sample. here we introduce confocal raman microspectroscopy as a method for investigating and illustrating the spatial heterogeneity of microbial cell popu ... | 2009 | 19475141 |
| a biochemically active mcm-like helicase in bacillus cereus. | the mini-chromosome maintenance (mcm) proteins serve as the replicative helicases in archaea and eukaryotes. interestingly, an mcm homolog was identified, by blast analysis, within a phage integrated in the bacterium bacillus cereus (bc). bcmcm is only related to the aaa region of mcm-helicases; the typical amino-terminus is missing and is replaced by a segment with weak homology to primases. we show that bcmcm displays 3'-->5' helicase and ssdna-stimulated atpase activity, properties that arise ... | 2009 | 19474351 |
| efficient decomposition of shrimp shell waste using bacillus cereus and exiguobacterium acetylicum. | two bacterial cultures were isolated and tested for degradation of shrimp shell waste. according to morphological examination, physiological tests, and applied molecular techniques, isolates were identified as bacillus cereus and exiguobacterium acetylicum. both strains were cultivated separately in flasks with 100 ml of shrimp shell waste broth (3% of washed, dried and ground shrimp shell waste in tap water, ph 7.0) at 37 degrees c. at determined periods of time, deproteinization and deminerali ... | 2009 | 19471983 |
| cold plasma technology: bactericidal effects on geobacillus stearothermophilus and bacillus cereus microorganisms. | cold plasma, also known as low temperature atmospheric pressure plasma (ltapp) is a novel technology consisting of neutral and charged particles, including free radicals, which can be used to destroy or inactivate microorganisms. research has been conducted regarding the effect of cold plasma on gram-positive bacteria; however, there is limited research regarding its ability to inactivate the spore-formers geobacillus stearothermophilus and bacillus cereus. | 2009 | 19470230 |
| rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method. | to develop a new, rapid and accurate reverse dot blot (rdb) method for the detection of intestinal pathogens in fecal samples. | 2009 | 19469006 |
| optimization of fermentation conditions for production of anti-tmv extracellular ribonuclease by bacillus cereus using response surface methodology. | bacillus cereus zh14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against tobacco mosaic virus. in order to enhance the ribonuclease production, in this study the optimization of culture conditions using response surface methodology was done. the fermentation variables including culture temperature, initial ph, inoculum size, sucrose, yeast extract, mgso(4).7h(2)o, and kno(3) were considered for selection of significant ones by u ... | 2010 | 19466461 |
| isolation and characterization of a protective bacterial culture isolated from honey active against american foulbrood disease. | bacterial strains isolated from us domestic honey were screened for antibacterial activity against paenibacillus larvae ssp. larvae, the causative agent of american foulbrood (afb) in apiaries. a bacterial isolate (th13) showing a high level of antimicrobial activity against p. larvae ssp. larvae atcc 9545 was selected and identified as paenibacillus polymyxa by 16s rrna gene sequencing. the antimicrobial compound was purified by 80% saturated ammonium sulfate precipitation followed by cm-sephar ... | 2009 | 19459943 |
| antimicrobial activity of benzophenones and extracts from the fruits of garcinia brasiliensis. | the pericarp and seeds from fruits of garcinia brasiliensis were subjected to extraction with hexane and ethanol. the pericarp hexane extract (phe) and seed ethanol extract (see) were purified by silica gel column chromatography, which permitted isolation of the prenylated benzophenones 7-epiclusianone (1) and guttiferone-a (2), respectively. the antimicrobial activity of phe, see, and compounds 1 and 2 were evaluated against candida albicans, staphylococcus aureus, escherichia coli, and bacillu ... | 2009 | 19459744 |
| enhancement of the activity and ph-performance of chitosanase from bacillus cereus strains by dna shuffling. | dna shuffling was carried out with two chitosanase genes belonging to glycoside hydrolase family eight from bacillus cereus knuc51 and b. cereus knuc55. the shuffled products, ym18 and ym20, which showed higher activity than the parents at 40 degrees c, were selected for further studies. the 50 kda chitosanases were purified using affinity chromatography with glutathione-sepharose 4b. in general, the specific activity of ym18 is enhanced 250% and that of ym20 is 350% compared to the parents. ym2 ... | 2009 | 19458918 |
| chemical composition and antimicrobial activity of essential oil of prangos ferulaceae (l.) lindl from iran. | the hydrodistilled essential oils of fruits and umbels of prangos ferulaceae (l.) lindl growing in iran were analysed by gas chromatograph-mass spectrometer. six compounds, comprising 93.7 and 94.7% of the total fruits and umbels oils, were identified, respectively. alpha-pinene and cis-ocimene are the major components of the oils. the essential oils show high antibacterial effect against bacillus cereus. | 2010 | 19452346 |
| biodegradation of international jet a-1 aviation fuel by microorganisms isolated from aircraft tank and joint hydrant storage systems. | microorganisms contaminating international jet a-1 aircraft fuel and fuel preserved in joint hydrant storage tank (jhst) were isolated, characterized and identified. the isolates were bacillus subtillis, bacillus megaterium, flavobacterium oderatum, sarcina flava, micrococcus varians, pseudomonas aeruginosa, bacillus licheniformis, bacillus cereus and bacillus brevis. others included candida tropicalis, candida albicans, saccharomyces estuari, saccharomyces cerevisiae, schizosaccharomyces pombe, ... | 2009 | 19452113 |
| antimicrobial activity of the methanolic extract and compounds from morus mesozygia stem bark. | this study was aimed at investigating the antimicrobial activity of the methanolic extract (mmb) and compounds isolated from the stem bark of morus mesozygia, namely 3beta-acetoxyurs-12-en-11-one (1), moracin q (2), moracin t (3), artocarpesin (4), cycloartocarpesin (5), moracin r (6), moracin u (8), moracin c (9), and moracin m (10). | 2009 | 19450674 |
| effects of gassericins a and t, bacteriocins produced by lactobacillus gasseri, with glycine on custard cream preservation. | lactobacillus gasseri la39 and la158 isolated from human-infant feces produce bacteriocins named gassericins a and t, respectively. both gassericins have high heat stability (121 degrees c, 10 min), good ph tolerance (ph 2-11), and strong bactericidality against many gram-positive bacteria, especially lactic acid bacteria, and thus are expected to be effective food preservatives. a microwell plate assay against 12 strains of custard cream spoilage bacteria showed that the gassericins had broader ... | 2009 | 19447968 |
| in situ detection of antibiotic-resistance elements in single bacillus cereus spores. | rapid detection of bacillus spores is a challenging task in food and defense industries. in situ labeling of spores would be advantageous for detection by automated systems based on single-cell analysis. determination of antibiotic-resistance genes in bacterial spores using in situ labeling has never been developed. most of the in situ detection schemes employ techniques such as fluorescence in situ hybridization (fish) that target the naturally amplified ribosomal rna (rrna). however, the major ... | 2009 | 19446419 |
| nosocomial bacteremia caused by biofilm-forming bacillus cereus and bacillus thuringiensis. | bacterial biofilms cause serious problems, such as antibiotic resistance and medical device-related infections. recent reports indicate that bacillus species potentially form biofilms and cause nosocomial bacteremia via catheter infection. our objective was to investigate the relationship between nosocomial bacteremia caused by bacillus species and biofilm formations. | 2009 | 19443973 |
| interventions for control of salmonella: clearance of microbial growth from rubber picker fingers. | a study was conducted to determine if a surface material with antimicrobial properties combined with an effective disinfectant could achieve total clearance of bacterial contamination. before beginning the project, new rubber picker fingers collected from 3 processing facilities were tested for endogenous microflora. five species of bacteria common to soil and human handling were present: bacillus amyloliquefaciens, bacillus cereus/thuringiensis, staphylococcus epidermidis, staphylococcus homini ... | 2009 | 19439642 |
| antibacterial protection by enterocin as-48 in sport and energy drinks with less acidic ph values. | the low ph and acid content found in sports and energy drinks are a matter of concern in dental health. raising the ph may solve this problem, but at the same time increase the risks of spoilage or presence of pathogenic bacteria. in the present study, commercial energy drinks were adjusted to ph 5.0 and challenged with listeria monocytogenes (drinks a to f), staphylococcus aureus, bacillus cereus, and bacillus licheniformis (drink a) during storage at 37 degrees c. l. monocytogenes was able to ... | 2009 | 19435243 |
| effect of different bacillus subtilis lipopeptides on surface hydrophobicity and adhesion of bacillus cereus 98/4 spores to stainless steel and teflon. | various lipopeptides produced by bacillus subtilis were examined for their ability to modify the surface hydrophobicity of two substrata, stainless steel (ss) and teflon. these modifications were evaluated by water contact angle measurements. the effects depended on the lipopeptide, its concentration, and the tested substratum. treatment of ss with different concentrations of surfactin s1 showed an increase of the hydrophobicity between 1 and 100 mg l(-1). on the same substratum, fengycin increa ... | 2009 | 19431000 |
| a novel mitochondrial sphingomyelinase in zebrafish cells. | sphingolipids are important signaling molecules in many biological processes, but little is known regarding their physiological roles in the mitochondrion. we focused on the biochemical characters of a novel sphingomyelinase (smase) and its function in mitochondrial ceramide generation in zebrafish embryonic cells. the cloned smase cdna encoded a polypeptide of 545 amino acid residues (putative molecular weight, 61,300) containing a mitochondrial localization signal (mls) and a predicted transme ... | 2009 | 19429680 |
| orf4 of the bacillus cereus sigb gene cluster encodes a general stress-inducible dps-like bacterioferritin. | the function of orf4 in the sigb cluster in bacillus cereus atcc 14579 remains to be explored. amino-acid sequence analysis has revealed that orf4 is homologous with bacterioferritins and dps. in this study, we generated an orf4-null mutant and produced recombinant protein rorf4 to establish the role of orf4. in vitro, the purified rorf4 was found to exist in two distinct forms, a dimeric form and a polymer form, through size exclusion analysis. the latter form exhibited a unique filament struct ... | 2009 | 19429618 |
| cereulide formation by bacillus weihenstephanensis and mesophilic emetic bacillus cereus at temperature abuse depends on pre-incubation conditions. | emetic toxin (cereulide) formation was recently identified in a psychrotolerant species, bacillus weihenstephanensis [thorsen, l., hansen, b.m., nielsen, k.f., hendriksen, n.b., phipps, r.k., budde, b.b., 2006. characterization of emetic bacillus weihenstephanensisis, a new cereulide-producing bacterium. applied and environmental microbiology, 72, 5118-5121.]. although recent findings indicated b. weihenstephanensis as a cereulide producer only limited information is available regarding environm ... | 2009 | 19428136 |
| ethanol and nacl susceptibility and protein expression of acid-adapted b. cereus 1-4-1 as well as its growth patterns in the presence of various carbon and nitrogen sources. | in the present study, the foodborne pathogen bacillus cereus 1-4-1 was subjected to acid adaptation treatment by suspending the test organism in tryptic soy broth (ph 5.5) for 2 hours. the susceptibility of the acid-adapted and nonadapted cells of b. cereus 1-4-1 to high concentrations of ethanol (20%) and nacl (20%) was then examined. in addition, the effect of acid adaptation on the protein expression profile of b. cereus 1-4-1 as well as the growth patterns of the acid-adapted and nonadapted ... | 2009 | 19425243 |
| formation of flavone di-o-glucosides using a glycosyltransferase from bacillus cereus. | microbial udp-glycosyltransferases can convert many small lipophilic compounds into glycons using uridinediphosphate- activated sugars. the glycosylation of flavonoids affects solubility, stability, and bioavailability. the gene encoding the udp-glycosyltransferase from bacillus cereus, bcgt-3, was cloned by pcr and sequenced. bcgt-3 was expressed in escherichia coli bl21 (de3) with a glutathione s-transferase tag and purified using a glutathione stransferase affinity column. bcgt-3 was tested f ... | 2009 | 19420995 |
| reaction pattern of bacillus cereus d-11 chitosanase on chitooligosaccharide alcohols. | the purified endochitosanase (mw 41 kda) from bacterium bacillus cereus d-11 hydrolyzed chitooligomers (glcn)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. the minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. to further investigate the cleavage pattern of this enzyme, chitooligosaccharide alcohols were prepared as substrates and the end products of hydrolysis were analyzed by tlc and hplc. the chitosanase split (glcn)4glcnoh into (glcn)3+ ... | 2009 | 19420990 |
| cloning, expression and purification of bacillus cereus endochitinase in the escherichia coli ad494(de3)plyss expression system. | a chitinase gene from bacillus cereus was cloned and expressed in escherichia coli. the purified recombinant chitinase had much higher (128 fold) specificity to pnp-beta-(glcnac)(3) than to pnp-beta-(glcnac), suggesting endochitinase. thirty-three amino acids in the n-terminal were recognized and cut off during expression, which consequently made the m(r) not correspond to that predicted. | 2009 | 19420688 |
| synthesis, spectral characterization, in-vitro microbiological evaluation and cytotoxic activities of novel macrocyclic bis hydrazone. | a macrocyclic hydrazone schiff base was synthesized by reacting 1,4-dicarbonyl phenyl dihydrazide with 2,6-diformyl-4-methyl phenol and a series of metal complexes with this new schiff base were synthesized by reaction with co(ii), ni(ii) and cu(ii) metal salts. the schiff base and its complexes have been characterized by elemental analyses, ir, (1)h nmr, uv-vis, fab mass, esr spectra, fluorescence, thermal, magnetic and molar conductance data. the analytical data reveal that the co(ii), ni(ii) ... | 2009 | 19419802 |
| a new rapid and sensitive detection method for cereulide-producing bacillus cereus using a cycleave real-time pcr. | to develop a rapid and sensitive detection method for cereulide-producing bacillus cereus using a real-time pcr based on the sequence of the cereulide synthesis gene. | 2009 | 19413807 |
| an overlap between the control of programmed cell death in bacillus anthracis and sporulation. | the staphylococcus aureus cid and lrg operons have been shown to control cell death and lysis in a manner thought to be analogous to programmed cell death (apoptosis) in eukaryotic organisms. although orthologous operons are present in a wide variety of bacterial species, members of the bacillus cereus group are unique in that they have a total of four cid-/lrg-like operons. two of these operons are similar to the s. aureus cid and lrg operons, while the other two (designated clhab(1) and clhab( ... | 2009 | 19411321 |
| [bacillus cereus sepsis and subarachnoid hemorrhage following consolidation chemotherapy for acute myelogenous leukemia]. | a 64-year-old man with acute myelogenous leukemia (fab classification, m7) in remission received consolidation chemotherapy with mitoxantrone/cytosine arabinoside. wbc counts decreased to 0/microl on day 14, and fever (39.3 degrees c) and epigastralgia developed on day 15. cefozopran was instituted for febrile neutropenia; however, on day 16, he was found to be in cardiac arrest. ct scan on day 16 revealed subarachnoid hemorrhage. gram-positive rods were isolated from blood cultures on day 15, a ... | 2009 | 19404024 |
| potential of nitrogen gas (n2) to control psychrotrophs and mesophiles in raw milk. | pure n(2) gas was introduced in the headspace of test bottles containing raw milk that were then stored at either 6.0, 7.0, or 12.0 degrees c. treatment with n(2) significantly reduced the growth of total bacteria, and the growth of bacterial subgroups such as psychrotrophs, enterobacteria, protease- and lipase-producing bacteria, and listeria spp, and completely excluded bacillus cereus growth on myp plates. the inhibitory effect was maximal at 6.0 degrees c, and bacterial growth could be halte ... | 2010 | 19398315 |
| phenotypic and transcriptomic analyses of mildly and severely salt-stressed bacillus cereus atcc 14579 cells. | bacteria are able to cope with the challenges of a sudden increase in salinity by activating adaptation mechanisms. in this study, exponentially growing cells of the pathogen bacillus cereus atcc 14579 were exposed to both mild (2.5% [wt/vol] nacl) and severe (5% [wt/vol] nacl) salt stress conditions. b. cereus continued to grow at a slightly reduced growth rate when it was shifted to mild salt stress conditions. exposure to severe salt stress resulted in a lag period, and after 60 min growth ha ... | 2009 | 19395575 |
| resde-dependent regulation of enterotoxin gene expression in bacillus cereus: evidence for multiple modes of binding for resd and interaction with fnr. | in the food-borne pathogen bacillus cereus f4430/73, the production of major virulence factors hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) is regulated through complex mechanisms. the two-component regulatory system resde is involved in the activation of hbl and nhe transcription. here, the response regulator resd and the sensor kinase rese were overexpressed and purified, and autophosphorylation of rese and transphosphorylation of resd by rese were demonstrated in vitro. resd is mainl ... | 2009 | 19395489 |
| zinc ion-induced domain organization in metallo-beta-lactamases: a flexible "zinc arm" for rapid metal ion transfer? | the reversible unfolding of metallo-beta-lactamase from chryseobacterium meningosepticum (blab) by guanidinium hydrochloride is best described by a three-state model including folded, intermediate, and unfolded states. the transformation of the folded apoenzyme into the intermediate state requires only very low denaturant concentrations, in contrast to the zn2-enzyme. similarly, circular dichroism spectra of both blab and metallo-beta-lactamase from bacillus cereus 569/h/9 (bcii) display distinc ... | 2009 | 19395380 |
| microbial co-habitation and lateral gene transfer: what transposases can tell us. | determining the habitat range for various microbes is not a simple, straightforward matter, as habitats interlace, microbes move between habitats, and microbial communities change over time. in this study, we explore an approach using the history of lateral gene transfer recorded in microbial genomes to begin to answer two key questions: where have you been and who have you been with? | 2009 | 19393086 |
| cloning of feather-degrading minor extracellular protease from bacillus cereus dcuw: dissection of the structural domains. | bacterial extracellular proteases play an important role in cell survival and cell-cell communication. a high-molecular-mass minor extracellular protease (vpr) from a feather-degrading bacterium, bacillus cereus dcuw, has been reported by our laboratory. in the present study, we cloned and expressed vpr in escherichia coli. complete nucleotide sequencing of this gene predicted that the protease is a member of the serine protease family, and smart domain analysis revealed that the protease consis ... | 2009 | 19383694 |
| lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations. | lipid rafts and ceramide (cer)-platforms are membrane domains that play an important role in several biological processes. cer-platforms are commonly formed in the plasma membrane by the action of sphingomyelinase (smase) upon hydrolysis of sphingomyelin (sm) within lipid rafts. the interplay among smase activity, initial membrane properties (i.e., phase behavior and lipid lateral organization) and lipid composition, and the amount of product (cer) generated, and how it modulates membrane proper ... | 2009 | 19383465 |
| a strain-variable bacteriocin in bacillus anthracis and bacillus cereus with repeated cys-xaa-xaa motifs. | bacteriocins are peptide antibiotics from ribosomally translated precursors, produced by bacteria often through extensive post-translational modification. minimal sequence conservation, short gene lengths, and low complexity sequence can hinder bacteriocin identification, even during gene calling, so they are often discovered by proximity to accessory genes encoding maturation, immunity, and export functions. this work reports a new subfamily of putative thiazole-containing heterocyclic bacterio ... | 2009 | 19383135 |
| in vitro selection of glms ribozymes. | riboswitches modulate gene expression in eubacteria and eukaryotes in response to changing concentrations of small molecule metabolites. in most examples studied to date, riboswitches achieve both metabolite sensing and gene control functions without the obligate involvement of protein factors. these findings validate the hypothesis that rna molecules could be engineered to function as designer gene control elements that sense and respond to different ligands. we believe that reverse engineering ... | 2009 | 19381572 |
| identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression. | riboswitches are rna elements capable of modulating gene expression through interaction with cellular metabolites. one member of the riboswitch family, the glms riboswitch, is unique among riboswitches in that it modulates gene expression by undergoing self-cleavage in the presence of its metabolite glucosamine-6-phosphate (glcn6p). in order to investigate the interactions between the glms rna and glcn6p we performed nucleotide analog interference mapping (naim) and suppression (nais). these tec ... | 2009 | 19381561 |
| lactobacillus isolates from weaned piglets' mucosa with inhibitory activity against common porcine pathogens. | twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (s. aureus, b. cereus, e. coli, c. perfringens). two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. isolates 4/26 and 2/25 (identified as l. crispatus or l. amylovorus) were inhibitory against most strains of s. aureus, b. cereu ... | 2009 | 19381487 |
| identification of surface proteins involved in the adhesion of a probiotic bacillus cereus strain to mucin and fibronectin. | several bacillus strains isolated from commercial probiotic preparations were identified at the species level, and their adhesion capabilities to three different model intestinal surfaces (mucin, matrigel and caco-2 cells) were assessed. in general, adhesion of spores was higher than that of vegetative cells to the three matrices, and overall strain bacillus cereus(ch) displayed the best adhesion. different biochemical treatments revealed that surface proteins of b. cereus(ch) were involved in t ... | 2009 | 19372165 |
| disinfection methods used in decontamination of bottles used for feeding powdered infant formula. | infant susceptibility and the risks posed by infections associated with bottle-fed powdered infant formula (pif) have received increased attention in recent years. intrinsic contamination of pif with pathogens has been reported and extrinsic contamination can be introduced from the handler or the environment during reconstitution. recommended disinfection advice and bottle decontamination have changed in recent years and the aim of this study was to validate the efficacy of four current disinfec ... | 2009 | 19370863 |
| construction of a recombinant intergenus multidomain chimeric protein for simultaneous expression of haemolysin bl of bacillus cereus, listeriolysin o of listeria monocytogenes and enterotoxin b of staphylococcus aureus. | haemolysin bl (hbl) of bacillus cereus, listeriolysin o (llo) of listeria monocytogenes and enterotoxin b (seb) of staphylococcus aureus are among the major toxin components contributing to the pathogenicity of these organisms in foodborne illnesses. in this study, an intergenus non-toxic multidomain fusion protein (r-hle) was generated with specificity for hbl, llo and seb. the fusion gene (r-hle) comprising the conserved regions of hbld and the hly and entb genes was codon-optimized for expres ... | 2009 | 19369518 |
| kinetics of bacterial phospholipase c activity at micellar interfaces: effect of substrate aggregate microstructure and a model for the kinetic parameters. | activity at micellar interfaces of bacterial phospholipase c from bacillus cereus on phospholipids solubilized in micelles was investigated with the goal of elucidating the role of the interface microstructure and developing further an existing kinetic model. enzyme kinetics and physicochemical characterization of model substrate aggregates were combined, thus enabling the interpretation of kinetics in the context of the interface. substrates were diacylphosphatidylcholine of different acyl chai ... | 2008 | 19367944 |
| ion binding properties and structure stability of the nak channel. | ion distribution in the selectivity filter and ion-water and ion-protein interactions of nak channel are systematically investigated by all-atom molecular dynamics simulations, with the tetramer channel protein being embedded in a solvated phospholipid bilayer. analysis of the simulation results indicates that k(+) ions prefer to bind within the sites formed by two adjacent planes of oxygen atoms from the selectivity filter, while na(+) ions are inclined to bind to a single plane of four oxygen ... | 2009 | 19366596 |
| formation and characterization of acyl carrier protein-linked polyketide synthase extender units. | polyketide natural products are assembled by the condensation of an initiating precursor, or starter unit, with a series of additional precursors referred to as extender units. while there are a number of polyketide synthase starter units, there are currently only seven known polyketide synthase extender units. polyketide synthase extender units thioesterified to coenzyme a have been known for some time; however, polyketide synthase extender units thioesterified to acyl carrier proteins (acps) h ... | 2009 | 19362639 |
| study of the antibacterial effects of chitosans on bacillus cereus (and its spores) by atomic force microscopy imaging and nanoindentation. | bacillus cereus is a gram-positive, spore-forming bacterium that is widely distributed in nature. its intrinsic thermal resistance coupled with the extraordinary resistance against common food preservation techniques makes it one of the most frequent food-poisoning microorganisms causing both intoxications and infections. in order to control b. cereus growth/sporulation, and hence minimize the aforementioned hazards, several antimicrobial compounds have been tested. the aim of this work was to a ... | 2009 | 19362422 |
| gold-silver nanoclusters having dipicolinic acid imprinted nanoshell for bacillus cereus spores recognition. | molecular imprinted polymers (mips) as a recognition element for sensors are increasingly of interest and mip nanoclusters have started to appear in the literature. in this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamidocysteine (mac) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. in this method, me ... | 2009 | 19362197 |
| reduction of salmonella enterica var. enteritidis colonization and invasion by bacillus cereus var. toyoi inclusion in poultry feeds. | the effect of continuously feeding the probiotic microorganism toyocerin to birds inoculated with salmonella enteritidis field-isolated strains on salmonella enteritidis prevalence, and performance variables were studied in 2 experiments. the experiments were performed with 1) broiler chickens in floor pens until slaughter 42 d of age, challenge was performed on d 3, 7, or 14 with 2 x 10(6) cfu per chick, and 2) single comb white leghorn chickens in cages until 28 d of age, challenge was perform ... | 2009 | 19359685 |
| copper(ii) and uranyl(ii) complexes with acylthiosemicarbazide: synthesis, characterization, antibacterial activity and effects on the growth of promyelocytic leukemia cells hl-60. | new chelates of n(1)-[4-(4-x-phenylsulfonyl)benzoyl]-n(4)-butyl-thiosemicarbazide (x=h, cl, br) with cu(2+) and uo(2)(2+) have been prepared and characterized by analytical and physico-chemical techniques such as magnetic susceptibility measurements, elemental and thermal analyses, electronic, esr and ir spectral studies. room temperature esr spectra of cu(ii) complexes yield {g} values characteristic of distorted octahedral and pseudo-tetrahedral geometry. infrared spectra indicate that complex ... | 2009 | 19356828 |
| engineered sers substrates with multiscale signal enhancement: nanoparticle cluster arrays. | defined nanoparticle cluster arrays (ncas) with total lateral dimensions of up to 25.4 microm x 25.4 microm have been fabricated on top of a 10 nm thin gold film using template-guided self-assembly. this approach provides precise control of the structural parameters in the arrays, allowing a systematic variation of the average number of nanoparticles in the clusters (n) and the edge-to-edge separation (lambda) between 1 < n < 20 and 50 nm < or = lambda < or = 1000 nm, respectively. investigation ... | 2009 | 19354266 |
| microbiological quality of spices marketed in the city of botucatu, são paulo, brazil. | the aim of this work was to assess the microbiological quality of spices sold in botucatu, são paulo, brazil. a total of 233 samples were analyzed for mesophilic bacteria, thermotolerant coliforms, bacillus cereus, staphylococcus aureus, and salmonella. data showed that 21 and 5.6% of these samples were not in agreement with the standards of brazilian law, due to an excess of coliforms and to the presence of salmonella, respectively. black pepper and cumin exhibited the lowest microbiological qu ... | 2009 | 19350991 |
| susceptibilities of bacillus subtilis, bacillus cereus, and avirulent bacillus anthracis spores to liquid biocides. | the susceptibility of spores of bacillus subtilis, bacillus cereus, and avirulent bacillus anthracis to treatment with hydrogen peroxide, peroxyacetic acid, a peroxy-fatty acid mixture, sodium hypochlorite, and acidified sodium chlorite was investigated. results indicated that b. cereus spores may be reasonable predictors of b. anthracis spore inactivation by peroxyacetic acid-based biocides. however, b. cereus was not a reliable predictor of b. anthracis inactivation by the other biocides. in s ... | 2009 | 19350981 |
| higher structure of cereulide, an emetic toxin from bacillus cereus, and special comparison with valinomycin, an antibiotic from streptomyces fulvissimus. | cereulide and valinomycin are both 36-membered cyclic depsipeptides with 12 stereogenic centers that have a very similar sequence of cyclo [-d-o-leu-d-ala-l-o-val-l-val-]3 and cyclo [-d-o-val-d-val-l-o-ala-l-val-]3, respectively. cereulide is an emetic toxin produced by bacillus cereus through an unusual non-ribosomal peptide synthesis (nrps), whereas valinomycin, produced by streptomyces fulvissimus, is a known antibiotic drug. both compounds are known as k+-ion-selective ionophores and cause a ... | 2009 | 19347893 |
| prevalence, genetic diversity, and antibiotic susceptibility of bacillus cereus strains isolated from rice and cereals collected in korea. | incidence and properties of bacillus cereus strains naturally present in cereals were evaluated by phenotypic characterization, antibiotic susceptibility testing, and pulsed-field gel electrophoresis. of 293 cereal samples tested, 73 (25%) contained b. cereus strains. incidence of b. cereus isolates varied with respect to sample; they were found in 15 (37%) of 83 brown rice samples, 23 (37%) of 63 glutinous rice samples, 16 (21%) of 76 barley samples, and 19 (27%) of 71 job's tears samples. all ... | 2009 | 19343952 |
| prevalence and toxigenic profiles of bacillus cereus isolated from dried red peppers, rice, and sunsik in korea. | bacillus cereus is a spore-forming foodborne pathogen responsible for diarrheal and emetic types of food poisoning. intoxication is caused by various enterotoxins or by emetic toxin. because of its widespread presence and the ability to form heat-stable endospores in a relatively short time, b. cereus has been difficult to control. in this study, 21 rice and 36 sunsik (a mixture of powdered raw grains) samples were examined for the prevalence of b. cereus. a multiplex pcr assay was used to evalu ... | 2009 | 19343947 |
| characterization of a bacteriocin produced by enterococcus faecalis n1-33 and its application as a food preservative. | a bacteriocin-producing strain, n1-33, isolated from fermented bamboo shoot was identified as enterococcus faecalis. the ph-adjusted culture supernatant of this strain consisted of several peptides with bacteriocin activity, and the supernatant inhibited the growth of pathogenic bacteria such as listeria monocytogenes. the major peptide with bacteriocin activity was purified, and the first 39 amino acid residues of the bacteriocin were found to be identical to enterocin mr10a produced by e. faec ... | 2009 | 19343940 |
| molecular cloning of cdnas encoding antimicrobial peptide precursors from the skin of the chinese brown frog, rana chensinensis. | skin plays a key role in the daily survival of amphibians. in the present study, six cdnas encoding amphibian skin antimicrobial peptide precursors from the chinese brown frog rana chensinensis, were cloned and identified as preprobrevinin-1cec, preprobrevinin-1ceb, preprotemporin-1cea, preprotemporin-1ceb, preprotemporin-1cec, and preprochensinin-1. preprotemporin-1cea, ceb, and cec are members of the temporin family, which are usually short, hydrophobic, and c-terminally alpha-amidated antimic ... | 2009 | 19341344 |
| proteomic analysis by two-dimensional differential gel electrophoresis (2d dige) of a high-pressure effect in bacillus cereus. | high hydrostatic pressure (hhp) is a new method used to reduce or eliminate microorganisms that are present in food. proteins are known to be the most important target of high pressure in living organisms. the main goal of this investigation was focused on the changes that occur on the proteins of bacillus cereus under hhp stress conditions. the two-dimensional differential gel electrophoresis (2d dige) technique allows for a simultaneous resolution of thousands of proteins based on fluorescent ... | 2009 | 19338277 |
| [contraction of the disordered loop located within c-terminal domain of the transcriptional regulator hlyiir causes its structural rearrangement]. | hlyiir is the negative transcriptional regulator of the hemolysin ii gene from bacillus cereus. previous x-ray studies showed that hlyiir contains a disordered loop (a.a. 170-185) located within the c-terminal domain near dimerization interface. to understand the influence of this region on hlyiir properties and for a potential improvement in the crystallogenesis of the hlyiir, we constructed a mutant of hlyiir in which this disordered region is substituted by a single alanine residue. biochemic ... | 2009 | 19334535 |
| determination of kinetic parameters in the biosorption of cr (vi) on immobilized bacillus cereus m(1)(16) in a continuous packed bed column reactor. | due to technological advancement, environment suffers from untreated toxic heavy metal bearing effluent coming from different industries. chromium (vi) is one of those heavy metals having adverse impact on ecological balance, human, and plant health because of its carcinogenic properties. biosorption is presented as an alternative to traditional technologies which are costly and inefficient for treatment of industrial wastes containing low amount of heavy metals. in this study, bioremediation of ... | 2009 | 19333567 |
| intergeneric coaggregation of non-flocculating acinetobacter spp. isolates with other sludge-constituting bacteria. | the ability of non-flocculating acinetobacter spp. isolates (a. johnsonii s35 and a. junii s33) to coaggregate with other sludge-constituting bacteria obtained from a sewage sludge sample was assessed by a spectrophotometric assay. a. johnsonii s35 showed significant coaggregation (aggregation index (ai)=70 to 99%) with 7 strains, bacillus cereus, blastomonas natatoria, mycobacterium sp., thermomonas brevis, and enterobacteriaceae family, identified by sequencing of 16s rrna genes. a. junii s33 ... | 2009 | 19332298 |
| antibacterial activities of dodonaea viscosa using contact bioautography technique. | the crude ethanolic extract and n-hexane, dichloromethane, ethyl acetate, n-butanol and aqueous fractions of dodonaea viscosa were analyzed for antibacterial potential against four gram positive bacteria: bacillus subtilis, bacillus cereus, micrococcus luteus, staphylococcus aureus, and three gram negative bacteria: escherichia coli, salmonella typhi, pseudomonas aeruginosa. preliminary screening showed inhibition against staphylococcus aureus, micrococcus luteus, escherichia coli and pseudomona ... | 2009 | 19325527 |
| crystal structure of bacillus cereus d-alanyl carrier protein ligase (dlta) in complex with atp. | d-alanylation of lipoteichoic acids modulates the surface charge and ligand binding of the gram-positive cell wall. disruption of the bacterial dlt operon involved in teichoic acid alanylation, as well as inhibition of the dlta (d-alanyl carrier protein ligase) protein, has been shown to render the bacterium more susceptible to conventional antibiotics and host defense responses. the dlta catalyzes the adenylation and thiolation reactions of d-alanine. this enzyme belongs to a superfamily of amp ... | 2009 | 19324056 |
| anthocyanins, antioxidative, and antimicrobial properties of american cranberry (vaccinium macrocarpon ait.) and their press cakes. | amounts of total phenolics, anthocyanins, and ascorbic acid in 4 american cranberry varieties harvested at 4 stages of maturity were measured. the larger amount of phenolic compounds was found in berries of "black veil" cultivar (504 mg/100 g) at ii stage of maturity. significantly larger amounts of anthocyanins were determined in the overripe berries of the cultivars "ben lear" and "black veil." the amount of ascorbic acid in berries increased during ripening from i to iii stage, and slightly d ... | 2009 | 19323730 |
| growth inhibition of foodborne and pathogenic bacteria by conjugated linoleic acid. | the influence of conjugated linoleic acid (cla) on the growth of some foodborne and pathogenic bacteria was examined. a potassium salt of cla (cla-k) was tested against three gram-positive strains ( bacillus cereus , staphylococcus aureus , and streptococcus mutans ) and five gram-negative strains ( pseudomonas aeruginosa , salmonella typhimurium , vibrio parahemolyticus , klebsiella pneumoniae , and proteus mirabilis ). cla-k-mediated growth inhibition was evident for all tested strains, partic ... | 2009 | 19317483 |
| a conserved 3' extension in unusual group ii introns is important for efficient second-step splicing. | the b.c.i4 group ii intron from bacillus cereus atcc 10987 harbors an unusual 3' extension. here, we report the discovery of four additional group ii introns with a similar 3' extension in bacillus thuringiensis kurstaki 4d1 that splice at analogous positions 53/56 nt downstream of domain vi in vivo. phylogenetic analyses revealed that the introns are only 47-61% identical to each other. strikingly, they do not form a single evolutionary lineage even though they belong to the same bacterial b cl ... | 2009 | 19304998 |
| distribution, diversity, and potential mobility of extrachromosomal elements related to the bacillus anthracis pxo1 and pxo2 virulence plasmids. | the presence of a pxo1- and/or pxo2-like plasmid(s) in clinical isolates of bacillus cereus sensu stricto and in strains of the biopesticide bacillus thuringiensis has been reported recently, and the pxo2-like plasmid pbt9727 and another pxo2-like plasmid, paw63, were found to be conjugative. in this study, a total of 1,000 b. cereus group isolates were analyzed for the presence of pxo1- and pxo2-like replicons and for the presence of pxo2-related conjugative modules. pxo1- and pxo2-like replico ... | 2009 | 19304837 |
| rhodomyrtone: a new candidate as natural antibacterial drug from rhodomyrtus tomentosa. | rhodomyrtone [6,8-dihydroxy-2,2,4,4-tetramethyl-7-(3-methyl-1-oxobutyl)-9-(2-methylpropyl)-4,9-dihydro-1h-xanthene-1,3(2h)-di-one] from rhodomyrtus tomentosa (aiton) hassk. displayed significant antibacterial activities against gram-positive bacteria including bacillus cereus, bacillus subtilis, enterococcus faecalis, staphylococcus aureus, methicillin-resistant s. aureus (mrsa), staphylococcus epidermidis, streptococcus gordonii, streptococcus mutans, streptococcus pneumoniae, streptococcus pyo ... | 2009 | 19303274 |
| characterization and purification of a new bacteriocin with a broad inhibitory spectrum produced by lactobacillus plantarum lp 31 strain isolated from dry-fermented sausage. | characterization and purification of a new bacteriocin produced by lactobacillus plantarum lp 31 strain, isolated from argentinian dry-fermented sausage. | 2009 | 19302495 |
| transcriptional regulation of metabolic pathways, alternative respiration and enterotoxin genes in anaerobic growth of bacillus cereus atcc 14579. | to assess genes specifically activated during anaerobic growth that are involved in metabolism and pathogenesis of the foodborne pathogen bacillus cereus. | 2009 | 19302486 |
| fructose and glucose mediates enterotoxin production and anaerobic metabolism of bacillus cereus atcc14579(t). | to determine the effects of carbohydrates on bacillus cereus atcc14579(t) anaerobic metabolism and enterotoxin production in amino acids rich medium. | 2009 | 19302315 |
| sequential secretion of collagenolytic, elastolytic, and keratinolytic proteases in peptide-limited cultures of two bacillus cereus strains isolated from wool. | to characterize the secretion of proteolytic activities against keratin, collagen and elastin in liquid cultures of bacillus cereus iz-06b and iz-06r isolated from wool. | 2009 | 19302303 |
| isolation, structure elucidation and antibacterial activities of streptothricin acids. | five streptothricin acids (compounds 1-5) were isolated by ion-exchange resin chromatography and preparative rp-hplc from the fermentation broth of streptomyces qinlingensis. their structures were elucidated mainly by analyses of the ir, hr-eis-ms and nmr spectral data. they were deduced as two known compounds, streptothricin f acid (1) and streptothricin d acid (2), and three new compounds, 12-carbamoylstreptothricin e acid (3), 12-carbamoylstreptothricin d acid (4) and n-acetyl-streptothricin ... | 2009 | 19300469 |
| effect of impeller speed and ph on the production of poly(3-hydroxybutyrate) using bacillus cereus spv. | p(3hb), is one of the most well studied polyhydroxyalkanoates. it is biodegradable, biocompatible, exhibits thermoplastic properties and can be produced from renewable carbon sources. the commercial exploitation of p(3hb) has been mainly held back by its high production costs. hence, a lot of research is required to optimize p(3hb) fermentation conditions. in this study we have focused on the effects of impeller speed and ph on p(3hb) production in bacillus cereus spv. four different impeller sp ... | 2009 | 19296657 |
| the diterpenoid ent-16-kauren-19-oic acid acts as an uncoupler of the bacterial respiratory chain. | ent-16-kauren-19-oic acid is a bacteriolytic diterpenoid present in the resin of the medicinal plant pseudognaphalium vira vira. the compound and its methyl ester showed strong activity against gram-positive bacteria, whereas the derivative 3beta-hydroxy-ent-kauren-19-oic acid was inactive against all assayed bacteria at the maximal concentration used (250 microg/ml). the bacteriolytic effect of ent-16-kauren-19-oic acid (5 microg/ml) was confirmed with cultures of bacillus cereus and staphyloco ... | 2009 | 19296427 |
| in vitro synergistic antibacterial activities of helvolic acid on multi-drug resistant staphylococcus aureus. | an agar plate method was established to screen synergistic antibacterial agents other than beta-lactamase inhibitors. by using this method, a strain aspergillus sp136 was selected for further studies. from the metabolites of this strain, a synergistic antibacterial compound was isolated by bioautographic tlc assay-guided fractionation and identified as helvolic acid. the synergistic effect of helvolic acid to penicillin was about 3 times that of clavulanic acid to penicillin in agar diffusion as ... | 2009 | 19296371 |
| comparative transcriptional profiling of bacillus cereus sensu lato strains during growth in co2-bicarbonate and aerobic atmospheres. | bacillus species are spore-forming bacteria that are ubiquitous in the environment and display a range of virulent and avirulent phenotypes. this range is particularly evident in the bacillus cereus sensu lato group; where closely related strains cause anthrax, food-borne illnesses, and pneumonia, but can also be non-pathogenic. although much of this phenotypic range can be attributed to the presence or absence of a few key virulence factors, there are other virulence-associated loci that are co ... | 2009 | 19295911 |
| a patient with acute myeloid leukemia who developed fatal pneumonia caused by carbapenem-resistant bacillus cereus. | bacillus cereus is known as a serious bacterial pathogen in neutropenic patients. b. cereus is often resistant to beta-lactams, including penicillins and cephalosporins. we report a case of fatal pneumonia caused by b. cereus in a patient with newly diagnosed acute myeloid leukemia (aml) during remission induction therapy. cefepime was started for febrile neutropenia (fn) initially and was switched to panipem/betamipron, when fulminant pneumonia supervened. the isolated strain was resistant not ... | 2009 | 19280299 |
| bioremediation of reclaimed wastewater used as landscape water by using the denitrifying bacterium bacillus cereus. | organic matter and nitrogen removal from reclaimed wastewater used as landscape water was carried out by in situ bioremediation. a denitrifying bacterium bacillus cereus dnf409 was introduced for this purpose, and the amount of b. cereus used was optimized. the total nitrogen (tn) content and chemical oxygen demand (cod) of the landscape water decreased from 9.86 to 3.1 mg/l (removal rate, 68.6%) and from 127 to 36 mg/l (removal rate, 71.7%). the transparency of water increased from 0.2 to 0.55 ... | 2009 | 19280091 |
| growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice. | food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ... | 2009 | 19278342 |
| characterization and identification of a novel marine streptomyces sp. produced antibacterial substance. | strain gb-2 is a marine microbe with broad-spectrum antimicrobial activity, isolated from soil taken from the coastal city lianyungang in the jiangsu province of china. analysis of its morphological, physiological, and biochemical characteristics as well as chemical components of the cell wall strongly suggested that the strain gb-2 belonged to the streptomyces sp. analysis of the nucleotide sequence of the 16s rrna gene of streptomyces sp. gb-2 strain showed a strong similarity (98%) with the 1 ... | 2009 | 19277782 |
| [technics of inactivating/removing medical chitosan pathogen]. | to verify the technics of inactivating/removing pathogens in medical chitosan derived from shrimp shell. | 2009 | 19275109 |
| secondary cell wall polysaccharides of bacillus anthracis are antigens that contain specific epitopes which cross-react with three pathogenic bacillus cereus strains that caused severe disease, and other epitopes common to all the bacillus cereus strains tested. | the immunoreactivities of hydrogen fluoride (hf)-released cell wall polysaccharides (hf-pss) from selected bacillus anthracis and bacillus cereus strains were compared using antisera against live and killed b. anthracis spores. these antisera bound to the hf-pss from b. anthracis and from three clinical b. cereus isolates (g9241, 03bb87, and 03bb102) obtained from cases of severe or fatal human pneumonia but did not bind to the hf-pss from the closely related b. cereus atcc 10987 or from b. cere ... | 2009 | 19270075 |
| sortase d forms the covalent bond that links bcpb to the tip of bacillus cereus pili. | bacillus cereus and other gram-positive bacteria elaborate pili via a sortase d-catalyzed transpeptidation mechanism from major and minor pilin precursor substrates. after cleavage of the lpxtg sorting signal of the major pilin, bcpa, sortase d forms an amide bond between the c-terminal threonine and the amino group of lysine within the ypkn motif of another bcpa subunit. pilus assembly terminates upon sortase a cleavage of the bcpa sorting signal, resulting in a covalent bond between bcpa and t ... | 2009 | 19269972 |
| molecular methods to evaluate biodiversity in bacillus cereus and bacillus thuringiensis strains from different origins. | the spore-forming genus bacillus includes species of industrial, clinical and environmental significance. the possibility of differentiating between bacillus cereus and bacillus thuringiensis, toxin producers associated with illness, is a real need in monitoring potentially contaminated foods to understand the real distribution of b. cereus/b. thuringiensis in different outbreak cases. as the use of dna comparison obtains clearer results than classical microbiological methods in distinguishing b ... | 2009 | 19269566 |
| nw-g01, a novel cyclic hexadepsipeptide antibiotic, produced by streptomyces alboflavus 313: i. taxonomy, fermentation, isolation, physicochemical properties and antibacterial activities. | nw-g01, a novel cyclic hexadepsipeptide antibiotic, was isolated by macroporous resin and silica gel column chromatography and hplc from the fermentation broth of strain no. 313. the producing strain was identified as streptomyces alboflavus on the basis of the morphological characteristics, physiological property and 16s rdna gene sequence analysis. nw-g01 exhibited strong antibacterial activity against bacillus subtilis, bacillus cereus, staphylococcus aureus, methicillin-resistant s. aureus, ... | 2009 | 19265870 |
| bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis. | the purpose of this study was to determine to what extent blood-retinal barrier (brb) permeability occurred during experimental bacillus cereus endophthalmitis and whether tight junction alterations were involved in permeability. | 2009 | 19264886 |
| effects of food composition on the inactivation of foodborne microorganisms by chlorine dioxide. | chlorine dioxide (clo2) is a strong oxidizing agent that can be applied in solution as well as in the gaseous state. it has bactericidal, fungicidal and viricidal properties. several food-related microorganisms, including gram-negative and gram-positive bacteria, yeasts, mould spores and bacillus cereus spores were tested for their susceptibility to 0.08 mg/l gaseous clo2 during 1 min at a relative humidity of 90%. in this screening, the resistance of the different groups of microorganisms towar ... | 2009 | 19254814 |
| siderophore-mediated iron acquisition systems in bacillus cereus: identification of receptors for anthrax virulence-associated petrobactin . | during growth under iron limitation, bacillus cereus and bacillus anthracis, two human pathogens from the bacillus cereus group of gram-positive bacteria, secrete two siderophores, bacillibactin (bb) and petrobactin (pb), for iron acquisition via membrane-associated substrate-binding proteins (sbps) and other abc transporter components. since pb is associated with virulence traits in b. anthracis, the pb-mediated iron uptake system presents a potential target for antimicrobial therapies; its cha ... | 2009 | 19254027 |
| dna as an adhesin: bacillus cereus requires extracellular dna to form biofilms. | the soil saprophyte bacillus cereus forms biofilms at solid-liquid interfaces. the composition of the extracellular polymeric matrix is not known, but biofilms of other bacteria are encased in polysaccharides, protein, and also extracellular dna (edna). a tn917 screen for strains impaired in biofilm formation at a solid-liquid interface yielded several mutants. three mutants deficient in the purine biosynthesis genes pura, purc, and purl were biofilm impaired, but they grew planktonically like t ... | 2009 | 19251901 |
| cereulide synthesis in emetic bacillus cereus is controlled by the transition state regulator abrb, but not by the virulence regulator plcr. | cereulide, a depsipeptide structurally related to the antibiotic valinomycin, is responsible for the emetic type of gastrointestinal disease caused by bacillus cereus. recently, it has been shown that cereulide is produced non-ribosomally by the plasmid-encoded peptide synthetase ces. using deletion mutants of the emetic reference strain b. cereus f4810/72, the influence of the well-known transcription factors plcr, spo0a and abrb on cereulide production and on the transcription of the cereulide ... | 2009 | 19246763 |
| identification of non-flagellar genes involved in swarm cell differentiation using a bacillus thuringiensis mini-tn10 mutant library. | swarming is a social phenomenon that enables motile bacteria to move co-ordinately over solid surfaces. the molecular basis regulating this process is not completely known and may vary among species. insertional mutagenesis of a swarming-proficient bacillus thuringiensis strain was performed, by use of the transposon mini-tn10, to identify novel genetic determinants of swarming that are dispensable for flagellation, swimming motility, chemotaxis and active growth. among the 67 non-swarming mutan ... | 2009 | 19246762 |
| thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. | thiopeptides, with potent activity against various drug-resistant pathogens, contain a characteristic macrocyclic core consisting of multiple thiazoles, dehydroamino acids, and a 6-membered nitrogen heterocycle. their biosynthetic pathways remain elusive, in spite of great efforts by in vivo feeding experiments. here, cloning, sequencing, and characterization of the thiostrepton and siomycin a gene clusters unveiled a biosynthetic paradigm for the thiopeptide specific core formation, featuring r ... | 2009 | 19246004 |
| bacillus cereus cell and spore properties as influenced by the micro-structure of the medium. | to investigate the effect of different growth conditions on bacillus cereus cell and spore properties. | 2009 | 19239549 |
| bacterial communities of tyre monofill sites: growth on tyre shreds and leachate. | to investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. | 2009 | 19239530 |
| antimicrobial effects of propolis extracts on escherichia coli and staphylococcus aureus strains resistant to various antibiotics and some microorganisms. | abstract propolis is collected by worker honey bees from the trees. the bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. propolis has also been used by humans since early times for various purposes and, especially, as a medicine because of its antimicrobial properties. in this study, antimicrobial effects of propolis samples on three escherichia coli and two staphylococcus aureus strains that are multiresistant to antibiotics and on salmonel ... | 2000 | 19236174 |
| the tubulin-like repx protein encoded by the pxo1 plasmid forms polymers in vivo in bacillus anthracis. | bacillus anthracis contains two megaplasmids, pxo1 and pxo2, that are critical for its pathogenesis. stable inheritance of pxo1 in b. anthracis is dependent upon the tubulin/ftsz-like repx protein encoded by this plasmid. previously, we have shown that repx undergoes gtp-dependent polymerization in vitro. however, the polymerization properties and localization pattern of repx in vivo are not known. here, we utilize a repx-green fluorescent protein (gfp) fusion to show that repx forms foci and th ... | 2009 | 19233922 |