Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influence of obesity on the spread of spinal analgesia after injection of plain 0.5% bupivacaine at the l3-4 or l4-5 interspace. | spinal anaesthesia was compared in 40 obese patients (increased body mass index (bmi] and 40 patients with normal bmi when 3 ml of plain 0.5% bupivacaine was injected at either the l3-4 or l4-5 interspace. more extensive cephalad spread of sensory block was achieved in patients with increased bmi compared with patients with normal bmi after injection at both l3-4 and l4-5 (p less than 0.05). the use of the l3-4 interspace instead of l4-5 resulted in a higher mean spread of block in patients with ... | 1990 | 2354091 |
| [voice rehabilitation following total laryngectomy: microvascular laryngeal replacement-plasty (laryngoplasty) instead of voice prosthesis]. | since the introduction of the so-called voice prostheses tracheoesophageal puncture is currently the most widely used surgical procedure for vocal rehabilitation after total laryngectomy. the voice prosthesis renders possible a reliably reproducible voice, which is superior (period of uninterrupted sound production, basal frequency, voice intensity) to the other techniques (esophageal speech, external vibrators, other surgical reconstructive measures), but has the following disadvantages: high i ... | 1990 | 2354015 |
| antimicrobial susceptibility testing of less commonly isolated haemophilus species using haemophilus test medium. | haemophilus test medium (htm) was developed recently for dilution and disk diffusion antimicrobial agent susceptibility testing of haemophilus influenzae. the application of htm to the testing of other, less frequently encountered haemophilus species recovered from humans was evaluated in this study by using commercially prepared htm (bbl microbiology systems, cockeysville, md.) in broth microdilution and agar disk diffusion susceptibility tests with 18 antimicrobial agents. a total of 93.3% of ... | 1990 | 2351742 |
| immunomodulatory role of thyroid hormones: in vitro effect of tetraiodothyronine (t4) on mitogen induced blastogenic response of lymphocytes. | an attempt was made to find out the immunomodulatory role of thyroid hormone, tetraiodothyronine (t4), and its effect on in vitro mitogen induced blastogenesis. human peripheral blood lymphocytes (pbl) were subjected to phytohaemagglutinin (pha) concanavalin-a (con. a) and pokeweed mitogen (pwm) in presence or absence of t4. basal blastogenic response was significantly enhanced in dose related manner by t4. pha and con.a induced response was depressed significantly (r = -0.975 and r = -0.945) wh ... | 1990 | 2351415 |
| [the alternation of cd4+ cells and its subpopulations by serial monitoring of peripheral blood lymphocytes of renal allograft recipients]. | immunologic monitoring for renal allograft recipients should be carried out serially and as conveniently as possible so that we can catch and treat the initial alternation of the rejection and infection. we have introduced the serial analysis of lymphocyte surface antigens of peripheral blood lymphocytes by flow cytometry for 9 cases of renal allograft recipients (3 cases of living related, 2 cases of living non-related, and 4 cases cadaver donors). in three recipients without rejection and infe ... | 1990 | 2351361 |
| the transport of thyroxine into mouse neuroblastoma cells, nb41a3: the effect of l-system amino acids. | thyroid hormones must cross the plasma membrane to interact with nuclear or other intracellular receptors. in brain cells, most of the t3 in the nucleus is derived intracellularly from t4. while a saturable transport system has been demonstrated for t3 in a number of cell types, the evidence for such a system for t4 is less well established. in a mouse neuroblastoma cell line (nb41a3) the transport of t4 was found to be stereospecific, saturable, and energy dependent. when cells were incubated w ... | 1990 | 2351115 |
| iodothyronine deiodinase activities in frtl5 cells: predominance of type i 5'-deiodinase. | frtl5 cells, a thyroid follicular cell line derived from normal rat thyroid, has been extensively used as a model system to study various aspects of the physiology of the thyroid epithelium. the capacity of these cells to metabolize iodothyronines and to generate t3 from t4 has not been previously examined. here we studied the deiodination of t4, t3, and rt3 in homogenates of frtl5 cells. by far, these homogenates were more potent catalyzing the 5'-deiodination (outer ring) of t4 and rt3 than th ... | 1990 | 2351108 |
| flavonoid administration immediately displaces thyroxine (t4) from serum transthyretin, increases serum free t4, and decreases serum thyrotropin in the rat. | naturally occurring and synthetic plant flavonoids, such as emd 21388, are potent inhibitors of thyroid hormone 5'-deiodinase (5'-d) in vitro, but not when given in vivo, since they are tightly bound by serum transthyretin (ttr). emd 21388 also inhibits the binding of t4 to human, dog, and rat serum ttr in vitro and when administered to rats in vivo. in the present studies the administration of emd 21388 inhibited the binding of t4 to ttr within 3 min, resulting in a decrease in the serum t4 con ... | 1990 | 2351100 |
| prognosis in laryngeal carcinoma: host factors. | a personal series of 1171 patients with a laryngeal tumour seen in a 27-year period from 1962 to 1988 is reported. of these patients, 763 were previously untreated and had a histologically proved squamous cell carcinoma. the interaction between the host factors (age, sex and general condition) and other tumour factors, and the effect of the host factors on survival were analysed. the maximum age incidence was the same in the two sexes but performance status (general condition) declined with incr ... | 1990 | 2350886 |
| homologous pairing in vitro initiated by dna synthesis. | a number of models have been proposed for the initiation of general genetic recombination. one of these, originally proposed by meselson and radding, imagines that the single-stranded 5' tail that results from strand displacement dna repair synthesis can initiate homologous recombination by invading a homologous duplex. the resultant d-loop intermediate is then processed into mature products. we demonstrate here that an in vitro system composed of the bacteriophage t4 uvsx protein (a reca-like " ... | 1990 | 2350351 |
| double-blind crossover trial of diltiazem versus propranolol in the management of thyrotoxic symptoms. | we compared the efficacy of diltiazem and propranolol in a randomized, prospective, double-blind, crossover study in six patients with untreated thyrotoxicosis (mean age 31 yrs). the patients received either diltiazem 60 mg orally four times a day or propranolol 40 mg orally four times a day, each for 1 week, separated by a 3-day drug-free period. blood pressure, heart rate, thyroid hormone levels (free t4, t3), electrocardiogram, two-dimensional and m-mode echocardiograms, and doppler studies w ... | 1990 | 2349134 |
| bacteriophage t4 gene 27. | 1990 | 2349100 | |
| compliance and laboratory data predict relapse rate of pneumocystis carinii pneumonia during prophylaxis with aerosol pentamidine. | we evaluated 43 aids patients on prophylaxis with aerosol pentamidine (60 mg biweekly) after pneumocystis carinii pneumonia (pcp). the effects of patients' inhalation compliance and of laboratory data during the initial pcp on subsequent pcp relapses were assessed. after a median of 8 months (range, 2-21.5 months) on pentamidine prophylaxis, 13 patients suffered a pcp relapse. six of them had missed at least one inhalation within the last month before the relapse. two of these six relapses were ... | 1990 | 2348643 |
| decreased sensitivity to alpha-adrenergic stimulation in hypothyroid patients. | nine hypothyroid patients had blood pressure and pulse rate responses to the alpha-adrenergic agonist phenylephrine measured before [t4 index, 45.045 +/- 9.009 nmol/l (mean +/- sem); tsh, 57.1 +/- 23.6 mu/l] and after 4 +/- 0.5 months of thyroid replacement therapy (t4 index, 141.570 +/- 29.601 nmol/l; tsh, 2.6 +/- 1.0 mu/l). hypothyroid patients had a smaller blood pressure increment and heart rate decrement at both 66.7 and 100 micrograms/min infusion rates of phenylephrine. furthermore, the s ... | 1990 | 2347907 |
| amiodarone does not affect the distribution and fractional turnover of triiodothyronine from the plasma pool, but only its generation from thyroxine in extrathyroidal tissues. | the peripheral metabolism of t3 has been studied in 10 hypothyroid patients receiving daily oral replacement doses of 200 micrograms t4 or 60 micrograms t3, in divided doses, before and during treatment with 400 mg amiodarone (amd) daily for 3 weeks. each patient received 1 microci/kg bw [125i]t3, iv. blood samples were drawn at 10, 20, 30, and 60 min and 2, 4, 6, 8, 12, 24, 36, 48, 60, and 72 h, followed by trichloroacetic acid precipitation and ethanol extraction of the serum samples. the para ... | 1990 | 2347903 |
| pulsatile secretion of thyrotropin during fasting: a decrease of thyrotropin pulse amplitude. | the effect of fasting on circadian and pulsatile tsh secretion was investigated in eight healthy subjects (four men and four women in the follicular phase). each subject was studied twice, once during 24 h with normal food intake and once during the last 24 h of a 60-h fast. blood was sampled every 10 min during 24 h for measurement of tsh by a sensitive immunoradiometric assay. fasting induced a decrease in plasma t3 [1.73 +/- 0.06 vs. 1.36 +/- 0.04 nmol/l; p less than 0.01 (mean +/- se), contr ... | 1990 | 2347898 |
| role of epidural analgesia on endocrine & metabolic responses to surgery. | serum levels of cortisol, t3, t4 and blood levels of glucose, lactate and pyruvate were measured 15 min before anaesthesia, 15 and 60 min after skin incision and at 2 h after surgery, in 16 patients undergoing elective surgery on lower extremities either under epidural analgesia (group i) or general anaesthesia (group ii). the results showed that as long as the effect of epidural analgesia persisted, it could inhibit the increases in cortisol and blood glucose and the decreases in t3 levels, obs ... | 1990 | 2347606 |
| the effect of extrathyroidal conversion inhibitor from food-deprived animals on iodothyronines deiodination by pituitary and cerebral cortical homogenates in vitro. | the influence of an inhibitor of iodothyronines' extrathyroidal conversion on t4, t3 and rt3 deiodination by adult pig pituitary and cerebral cortical homogenates has been investigated. the homogenates were incubated with t4, t3 and rt3 in the presence of 5 mm dithiothreitol and evaporated diethyl ether extracts of sera obtained from fed and starved (1-14 days) rabbits. the extracts had no influence either on t4 to t3 or on t4 to rt3 conversion in cerebral cortex. deiodination of rt3 to 3,3'-t2 ... | 1990 | 2347541 |
| comparison of atrophic and goitrous auto-immune thyroiditis in children: clinical, laboratory and tsh-receptor antibody studies. | we studied the clinical features, laboratory and thyroid functions and thyrotropin (tsh)-receptor and thyroid-stimulation antibodies in 21 patients with atrophic auto-immune thyroiditis (aat) and 48 patients with goitrous auto-immune thyroiditis (gat) of childhood onset. the clinical features of patient with aat were cessation of growth and obesity, while asymptomatic enlargement of the thyroid gland was the sole symptom in most patients with gat. although the ages at diagnosis were comparable i ... | 1990 | 2347350 |
| the many faces of sarcoidosis. the thomé villar memorial lecture. | sarcoidosis wears many different faces, depending upon the viewer, who may be clinician, radiologist, pathologist or immunologist. this thomé villar memorial lecture salutes the charismatic chest physician who pioneered studies of the disorder in lisbon and transmitted this knowledge internationally. the four main themes of this lisbon conference are granuloma formation, granulomatous vasculitis, markers of activity and occupational granulomatous lung diseases; these four faces of sarcoidosis ar ... | 1990 | 2345814 |
| leakage of thyroid hormone-inducible monoamine oxidase inhibitor from rat liver cytosol. | t4-induced endogenous monoamine oxidase (mao) inhibitor in rat liver perfusate by perfusion with sucrose buffer and lipid peroxide (lpo) and superoxide dismutase (sod) in rat liver tissues were investigated after pretreatment with total hepatic ischemia. when the gel filtration of these rats liver perfusate were carried out using sephadex g-25, fraction 18-20 were found to inhibit mao activity. lpo of these rats were increased, but sod activity was decreased. the accumulation of lipid peroxides ... | 1990 | 2345806 |
| heat mutagenesis in bacteriophage t4: another walk down the transversion pathway. | extracellular nonreplicating bacteriophage t4 particles accumulate mutations as functions of temperature, time, ph, and ionic environment via two mechanisms: 5-hydroxymethylcytidine deamination produces g.c----a.t transitions while a guanosine modification produces transversions. neither frameshift mutations nor mutations at a.t base pairs are appreciably induced. we now show that heat induces g.c----t.a transversions which we suggest may arise via a g*.a mispair, in which g* is a modified guano ... | 1990 | 2345133 |
| [products of expression of cloned phage t4 basal plate genes 29, 28, 27, 51 and 26]. | we used bacterial strains with two recombinant plasmids carrying bglii dna fragment with t4 genes 25-29 in different orientations. in the case of the plasmid prl705 phage dna fragment had correct orientation for transcription of t4 late genes, and in the plasmid prl707 it has the opposite one. transcription from the pl promoter of the plasmid prl705 led to the synthesis of 4 proteins which according to the molecular weights observed were the products of t4 genes 29, 28, 27, and 51. in the case o ... | 1990 | 2344958 |
| the effect of d- versus l-propranolol in the treatment of hyperthyroidism. | the purpose of this study is to determine whether there is a difference in treatment of hyperthyroidism using either the d- or l-isomer of propranolol. two groups of 20 patients with overt hyperthyroidism received either 120 mg l- or d-propranolol each for a period of 5 days. in the d-propranolol administered group there was a significant decrease in tt3 and ft3 plasma levels and in the ratio of tt3 to tt4; however, a significant increase occurred in rt3 values up to day 5. on the other hand, l- ... | 1990 | 2344697 |
| [prospective study of hiv infection in a group of 30 young hemophiliacs]. | in order to investigate the progression to aids, we studied 39 patients with hemophilia ranging presently in age from 8 to 20 years, who had been positive for hiv antibodies for a mean of 36 months. every 3 to 6 months a clinical and laboratory examination was performed. the number of patients with aids increased from 2 to 6 and 2 patients died from opportunistic infections. the percentage of patients with less than 500 t4 cells increased from 28% to 41%. increase in serum iga concentrations abo ... | 1990 | 2344245 |
| the effect of 0.5% ropivacaine on epidural blood flow. | twenty patients scheduled for elective abdominal surgery received epidural analgesia with 20 ml 0.5% ropivacaine or 0.5% bupivacaine. epidural blood flow was measured by an epidural 133xe clearance technique on the day before surgery (no local anaesthetic) and again 1 h before surgery, 30 min after injection of the local anaesthetic during continuous infusion (8 ml/h). median initial blood flow was 5.0 ml/min and 6.0 ml/min per 100 g tissue in patients receiving ropivacaine and bupivacaine, resp ... | 1990 | 2343734 |
| [the lymphocyte populations at different stages in the infection caused by the human immunodeficiency virus]. | in 58 persons infected with hiv the comparative study of the content of immunocompetent cells was carried out by the methods of rosette formation and immunofluorescence with the use of monoclonal antibodies. an increase in the content of rosette-forming cells (rfc), such as active e-rfc, e-rfc, theophylline-resistant e-rfc and m-frc, in asymptomatic seropositive persons was established. at this stage of the disease the amount of lymphocytes t4 and t8 and their ratio underwent no essential change ... | 1990 | 2343681 |
| pituitary regulation of the male-specific steroid 6 beta-hydroxylase p-450 2a (gene product iiia2) in adult rat liver. suppressive influence of growth hormone and thyroxine acting at a pretranslational leve;. | oligonucleotide probes that distinguish between two closely related mrnas encoding steroid 6 beta-hydroxylases of rat p-450 gene family cyp3a were used to individually assess their responsiveness to pituitary hormone regulation. northern blot analysis revealed that the elevation of immunoreactive p-450 iiia2 in livers of hypophysectomized rats reflects an elevation of the constitutive, male-specific p-450 iiia2 (p-450 2a) and not an induction of the drug-inducible p-450 iiia1 (p-450p). p-450 iii ... | 1990 | 2342479 |
| an rna holliday junction? structural and dynamic considerations of the bacteriophage t4 gene 60 interruption. | a novel interrupted gene motif has been reported in which a 50-nucleotide insertion into bacteriophage t4 gene 60 appears to be present in the message at the time of translation, yet it is not translated. we present here a dynamic model for how translation may be occurring in the neighborhood of the interruption. the model involves formation of an rna structure with similarities to a holliday junction, followed by migration of the branch point in a strand exchange between message and interruptio ... | 1990 | 2342100 |
| [correlation of diameters measured by x-ray computed tomography and intraparietal extension of squamous cell carcinoma of the thoracic esophagus. apropos of 101 anatomoradiologic comparisons]. | in order to assess the value of tomodensitometric classification of squamous cell carcinomas of the upper esophagus, which has been recently advocated, we compared the ct-measured diameters and the extent of intraparietal invasion in 101 tumors. according to selected criteria (superficial -t1- tumors: not apparent or less than 10 mm; tumors infiltrating incompletely the muscularis -t2- ; mass less than 10 to less than 20 mm in diameter; tumors extending beyond the muscularis -t3, t4- : diameter ... | 1990 | 2341503 |
| spect gallium-67 scanning in early human immunodeficiency virus (hiv) infection. failure of scanning abnormalities to correlate with immunologic staging. | the use of gallium scanning in the treatment of patients with aids has been well described. in this study, 27 hiv-infected patients (walter reed staging classification i-v) with normal chest roentgenograms were evaluated to determine the occurrence of thoracic gallium abnormalities in early hiv infection. spect was used for gallium scanning. patients received gallium injection on day 1 and bronchoalveolar lavage on day 2, and scanning was performed on day 3. twenty-eight scans in 27 patients wer ... | 1990 | 2340670 |
| neuromedin u-like immunoreactivity in the thyroid gland of the rat. | neuromedin u is a novel neuropeptide found to have a widespread distribution extending throughout the mammalian central nervous system, gastrointestinal tract and the endocrine cells of the pituitary gland. in order to investigate the possibility that neuromedin u-like immunoreactivity is also present in the thyroid gland of the adult rat we have examined its localisation and molecular nature by radioimmunoassay, immunocytochemistry and chromatographic analysis. the neuromedin u content of the w ... | 1990 | 2340578 |
| analysis of t lymphocyte subsets in all stages of diabetes. | direct immunofluorescence analysis of circulating blood lymphocytes from patients with all stages of type i diabetes revealed marked phenotypic abnormalities among all major t-lymphocyte subpopulations. the alterations were most pronounced in the prediabetics and comprised a stimulation of the t4+2h4+ and t8+2h4+ suppressor inducer subpopulations and corresponding depression of the t4+4b4+ helper inducers. | 1990 | 2340061 |
| tissue-specific regulation of the expression of rat kallikrein gene family members by thyroid hormone. | we have altered the thyroid hormonal status of both male and female rats and examined the expression of six functional members of the rat kallikrein gene family (ps, s1, s2, s3, k1 and p1) in the submandibular gland (smg), kidney, prostate, testis and anterior pituitary gland (ap) of these animals. on northern-blot analysis with gene-specific oligonucleotide probes, the steady-state mrna levels of s1, s2, s3, k1 and p1 were all dramatically altered in the smg of male and female rats treated with ... | 1990 | 2339984 |
| [cancer of the lips. results of the treatment of 299 patients]. | from 1970 to 1985, 299 patients with carcinoma of the lip were entirely managed at the bergonié foundation. interstitial radiotherapy was most commonly used. it resulted in a successful local control in 96% of cases (238/248), while cosmetic and functional results seemed better than those of surgery. however, surgery seemed more performant for in situ carcinomas, late aggressive t3 carcinomas, local recurrences, as for t4 lesions associated with other techniques. after reviewing the case history ... | 1990 | 2339862 |
| prostaglandins in squamous cell carcinoma of the larynx: tumor and peritumor synthesis. | prostaglandin (pg) e2, 6ketopgf1 alpha and thromboxane b2 (txb2) production by the tumor, peritumor and control tissue were investigated in specimens from patients (n = 11) with squamous cell carcinoma of the larynx, in relation to the extension and infiltration of the neoplasm and to the presence of inflammation, fibrosis and necrosis. in all specimens detectable amounts of 6ketopgf1+ and txb2 were found, but the predominant metabolite was pge2. no differences in the levels of txb2 and 6ketopgf ... | 1990 | 2339137 |
| serum concentrations of thyroid hormones and activity of iodothyronine deiodinase in peripheral tissues of the house musk shrew, suncus murinus. | serum concentrations of thyroid hormones and the properties of iodothyronine deiodinase in tissues of the house musk shrew, suncus murinus, were examined and compared with those of the rat. serum concentrations of thyroxine (t4) and 3,3',5'-tri-iodothyronine (rt3) were higher, while the serum concentration of 3,5,3'-tri-iodothyronine (t3) was lower in the shrew than in the rat. among liver, kidney, skeletal muscle, heart, brown adipose tissue (bat), spleen, lung, testes and thymus homogenates of ... | 1990 | 2338528 |
| presence of thyroxine 5'-monodeiodinase activity in kidney homogenates of the frog, rana ridibunda. | conversion of thyroxine (t4) to triiodothyronine (t3), which was demonstrated in the 6000-g supernatant fraction of kidney homogenates prepared from rana ridibunda frogs, was influenced by substrate (0.19-1.5 microm) and homogenate (39-625 mg kidney/ml) concentration and by temperature (19.5-36.5 degrees) and duration (1 hr vs 2 hr) of incubation. heating destroyed 5'-monodeiodinase activity. addition of dithiothreitol (dtt) to the reaction mixture appeared to be essential to quantitate t3 produ ... | 1990 | 2338224 |
| [subtotal thyroid gland resection as therapy for thyrotoxic crises]. | thyrotoxic crises occurred in six patients (four women aged 51, 63, 72 and 76 years; two men aged 52 and 63 years). in four patients the crisis was triggered by a contrast medium containing iodine, and in one by amiodarone. the cause of the crisis in the 51-year-old woman remained uncertain. after a latent period of up to two months, t3 and t4 concentrations rose in all the patients, and abnormal findings such as tachycardia, increased blood pressure, dehydration, tremor, restlessness, hallucina ... | 1990 | 2338059 |
| ph-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of t4 lysozyme. | the energetics of a salt bridge formed between the side chains of aspartic acid 70 (asp70) and histidine 31 (his31) of t4 lysozyme have been examined by nuclear magnetic resonance techniques. the pka values of the residues in the native state are perturbed from their values in the unfolded protein such that his31 has a pka value of 9.1 in the native state and 6.8 in the unfolded state at 10 degrees c in moderate salt. similarly, the aspartate pka is shifted to a value of about 0.5 in the native ... | 1990 | 2337607 |
| serum thyrotropin in hospitalized psychiatric patients: evidence for hyperthyrotropinemia as measured by an ultrasensitive thyrotropin assay. | in order to gather further insight into the basis for high serum t4 and/or t3 of psychiatric illnesses, we studied thyroid function in 84 consecutive newly hospitalized psychiatric patients (hpp) in a 12-week period. serum t4 and t3 were measured by immunoassay and thyrotropin (thyroid-stimulating hormone [tsh]) by an ultrasensitive immunoradiometric assay. serum t4 was in the normal range in 64 (76%) and elevated in 20 (24%); free t4 index was elevated in 13 of 75 (16%), total t3 in 12 of 60 (2 ... | 1990 | 2336036 |
| does a hidden pool of reverse triiodothyronine (rt3) production contribute to total thyroxine (t4) disposal in high t4 states in man. | a hidden pool of rt3 production represents a source of rt3 that is minimally reflected in circulating rt3 levels. to test for the existence of such a source of rt3 production in man, varying doses of the generalized deiodinase inhibitor iopanoic acid (ia) were administered to four hyperthyroxinemic subjects. the doses employed included low-ia (0.5-g load, then 0.5 g/day for 5 days), mid-ia (1.0-g load, then 1.0 g/day for 5 days), and high-ia (3.0-g load, then 3.0 g/day for 5 days). each patient ... | 1990 | 2335581 |
| changes in thyroid status in newborn infants utero-exposed to ritodrine. | a clinical study to assess whether utero exposure to ritodrine influences thyroid status was performed in 21 healthy term newborn infants, 10 exposed in utero to ritodrine (treated group) and 11 non-exposed in utero to drugs (control group). the treated group had a t3/t4 ratio significantly higher than the control group (mean +/- sd: 124.10 +/- 23.70 vs. 96.09 +/- 18.11, p less than 0.005) and t3 slightly increased (mean +/- sd nmol/l 2.48 +/- 0.69 vs. 1.95 +/- 0.56). the mean serum values of th ... | 1990 | 2335252 |
| effect of gossypol on thyroid hormones in young female rats. | the study was designed to examine the effect of gossypol on thyroid function in young female rats. forty young sprague-dawley female rats were randomly assigned to four treatment groups. the dosages of gossypol acetic acid (gossypol) used for this experiment were 1, 5 and 10 mg/kg body weight/day in 0.5 ml potassium phosphate buffer subcutaneously started from day 34 of age for 15 consecutive days. the control group was treated with vehicle alone. animals were sacrificed 15 days after gossypol t ... | 1990 | 2335106 |
| detection of 1,n6-propanodeoxyadenosine in acrolein-modified polydeoxyadenylic acid and dna by 32p postlabeling. | the interaction of acrolein, an alpha,beta-unsaturated aldehyde, with polydeoxyadenylic acid and dna has been investigated using 32p-postlabeling analysis. in preliminary experiments, polydeoxyadenylic acid was incubated with excess acrolein and then digested to 3' monophosphates prior to transfer of 32p from [gamma-32p]atp with t4 polynucleotide kinase. the 3',5'-bisphosphates were 3'-dephosphorylated prior to two-dimensional thin layer chromatography on polyethyleneimine-cellulose layers. auto ... | 1990 | 2334905 |
| effect of an engineered disulfide bond on the folding of t4 lysozyme at low temperatures. | equilibrium and kinetic effects on the folding of t4 lysozyme were investigated by fluorescence emission spectroscopy in cryosolvent. to study the role of disulfide cross-links in stability and folding, a comparison was made with a mutant containing an engineered disulfide bond between cys-3 (ile-3 in the wild type) and cys-97, which links the c-terminal domain to the n terminus of the protein [perry & wetzel (1984) science 226, 555]. in our experimental system, stability toward thermal and dena ... | 1990 | 2334694 |
| structure of a thermostable disulfide-bridge mutant of phage t4 lysozyme shows that an engineered cross-link in a flexible region does not increase the rigidity of the folded protein. | a disulfide bond introduced between amino acid positions 9 and 164 in phage t4 lysozyme has been shown to significantly increase the stability of the enzyme toward thermal denaturation [matsumura, m., becktel, w.j., levitt, m., & matthews, b. w. (1989) proc. natl. acad. sci. u.s.a. 86, 6562-6566]. to elucidate the structural features of the engineered disulfide, the crystal structure of the disulfide mutant has been determined at 1.8-a resolution. residue 9 lies in the n-terminal alpha-helix, wh ... | 1990 | 2334683 |
| evaluation of the sensitivity of microfilter paper assays in an anthropological study: results of samples from cameroon and tanzania. | microfilter absorbed whole blood samples from 223 tanzanian babies and 189 adults from cameroon have been examined. blood specimens are difficult to obtain from african suburban and rural areas, and lack of storage and transportation facilities can prevent the collection of samples. we evaluated some microassays employing whole blood collected on filter paper. this method is a well established technique in neonatal screening for endocrinometabolic diseases. we also developed microassays for whol ... | 1990 | 2334145 |
| is crystalloid preloading useful in spinal anaesthesia in the elderly? | sixty asa grade 1 or 2 patients, aged 60 years or over, scheduled for surgery to the lower abdomen or lower limbs under spinal anaesthesia were allocated randomly to one of three treatment groups. group a received 16 ml/kg of ringer's acetate solution immediately before spinal anaesthesia, group b received 8 ml/kg and group c received no volume preload. heart rate, arterial pressure and anaesthetic level were recorded by an independent observer. the overall incidence of systemic arterial hypoten ... | 1990 | 2334037 |
| thyroidal and peripheral production of 3,5,3'-triiodothyronine in humans by multicompartmental analysis. | multicompartmental analysis of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) kinetics based on the plasma disappearance curves of the two tracer hormones (j. j. distefano iii, m. jang, t. k. malone, and m. broutman. endocrinology 110: 198-213, 1982 and j. j. distefano iii, t. k. malone, and m. jang. endocrinology 111: 108-117, 1982) was extended to include additional experimental data, namely, the appearance curve in plasma of labeled t3 generated in vivo from precursor t4. kinetic analysis of ... | 1990 | 2333963 |
| vertical transmission of human immunodeficiency virus is correlated with the absence of high-affinity/avidity maternal antibodies to the gp120 principal neutralizing domain. | many, but not all, infants born to mothers infected with the human immunodeficiency virus (hiv) are infected in utero. we have now shown that mothers who have high-affinity/avidity antibodies directed toward the principal neutralizing domain (pnd) of gp120 are less likely to transmit hiv to their children. an elisa that preferentially measures the level of the biologically functioning, high-affinity/avidity antibodies against pnd is described. in a retrospective study of 15 maternal/neonatal ser ... | 1990 | 2333294 |
| small colloidal gold conjugated to fab fragments or to immunoglobulin g as high-resolution labels for electron microscopy: a technical overview. | fab-colloidal gold labelling in conjunction with negative staining and high-resolution electron microscopy was used for targeting single protein units in regular arrays. these were bacteriophage t4 polyheads with fab-au2.5, and a specific antibody binding site on the haemagglutinin polypeptide of influenza virus with fab-au3, fab-au2.5, and fab-au1-2. for the latter, igg-au3 was also used. experimental details are summarized to provide generally applicable methods for the preparation of small go ... | 1990 | 2332806 |
| il-1 beta modulation of spontaneous autoimmune diabetes and thyroiditis in the bb rat. | long term effects of in vivo treatment with human ril-1 beta on diabetogenesis and thyroid disease were determined in the biobreeding rat. administration of high dose (10 micrograms/kg) il-1 beta accelerated the onset of insulin-dependent diabetes mellitus compared to saline-injected controls. high dose treatment resulted in goiter development, pronounced lt, reduced serum t4 levels, and overall growth reduction. in contrast, low dose il-1 beta (0.5 microgram/kg) administration significantly red ... | 1990 | 2332631 |
| the acute effects of food and glucose challenge on plasma thyroxine and triiodothyronine levels in previously starved rainbow trout (oncorhynchus mykiss). | the acute effects of a single meal on plasma l-thyroxine (t4) and 3,5,3'-triiodo-l-thyronine (t3) levels were examined in rainbow trout starved for 3 days. plasma t4 increased within 2 hr of food intake and remained elevated to 8 hr. plasma t3 was not altered consistently. feeding-induced elevations in plasma t4 were present only in trout weighing less than 250 g and if they consumed a ration exceeding 0.38% of body weight. postprandial elevations in plasma glucose paralleled those in plasma t4, ... | 1990 | 2332149 |
| [the effect of diet therapy on the hormonal spectrum of patients with bronchial asthma]. | results are reported of an investigation of the levels of cortisol, pancreatic and thyroid hormones in 38 patients with the infectious-allergic form of bronchial asthma. unloading dietotherapy was accompanied by an improvement of the clinical course of the disease, reduction of necessity in broncholytic and hormonal agents. during the unloading dietotherapy period some variants of hormonal response to hunger were revealed. patients with a severe course of the acidotic crisis revealed a reduction ... | 1990 | 2330711 |
| [complications of the postoperative use of lumbar epidural catheters in place for surgery]. | two cases of secondary subarachnoidal dislocation of lumbar epidural catheters are reported. the first catheter was placed, tested, and injected with 20 ml local anesthetic in order to achieve intraoperative anesthesia up to the t4 level. on the 2nd postoperative day the test dose produced paralysis of the lower extremities; liquor now could be aspirated. the second catheter had been introduced into the epidural space immediately after injection of 15 ml local anesthetic through the needle. use ... | 1990 | 2330430 |
| [the functional links in the neuroendocrine interrelationship in juvenile uterine hemorrhages]. | a simultaneous and combined study of function of the neuroendocrine regulation of menstrual function in patients with juvenile uterine bleeding (jub) (by the excretion of lh, noradrenalin, dopa and dopamine with urine and by the blood levels of tsh, t3, t4, acth and cortisol and by functional diagnostic tests) has shown that jub is characterized by dysfunction of all important factors in this complex neuro-regulatory chain, resulting in disorder of hormonal homeostasis and pathological changes i ... | 1990 | 2330360 |
| thyroid hormone transport in a human glioma cell line. | the uptake of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) was studied in human glioma cells (hs 683) and compared with that in several other neural cell lines. at 25 degrees c or 37 degrees c, total cell uptake rose rapidly and reached equilibrium within 60 min. the glioma cells had the highest uptake: 47.6 fmol of l-t3 and 43.4 fmol of l-t4 per 10(6) cells at 37 degrees c. these were inhibited 77% and 72%, respectively, by excess unlabeled hormone. uptake in the nuclei reached equilibrium b ... | 1990 | 2328825 |
| contribution of maternal thyroxine to fetal thyroxine pools in normal rats near term. | normal dams were equilibrated isotopically with [125i]t4 infused from 11 to 21 days of gestation, at which time maternal and fetal extrathyroidal tissues were obtained to determine their [125i]t4 and t4 contents. the specific activity of the [125i]t4 in the fetal tissues was lower than in maternal t4 pools. the extent of this change allows evaluation of the net contribution of maternal t4 to the fetal extrathyroidal t4 pools. at 21 days of gestation, near term, this represents 17.5 +/- 0.9% of t ... | 1990 | 2328706 |
| calcium is the first messenger for the action of thyroid hormone at the level of the plasma membrane: first evidence for an acute effect of thyroid hormone on calcium uptake in the heart. | the thyroid hormone t3 produced a very rapid and transient increase in 45calcium uptake by freshly isolated rat heart slices, which was seen already 15 sec after the addition of the hormone, reached a maximum at 30 sec, and then progressively declined and returned to control values after 10 min. this effect of t3 was independent of extracellular calcium, concentration related (evident at a physiological concentration of 10 pm, reached maximum of about 75% above control at 1 nm, and was smaller a ... | 1990 | 2328703 |
| regulation of iodothyronine 5'-deiodinases: effects of thiol blockers and altered substrate levels in vivo and in vitro. | to study the physiological regulation of the iodothyronine 5'-deiodinases (i-5'd), we have examined the effects of some thiol blockers and of thyroid status on i-5'd activities both in vitro and in vivo. at low (less than 5 mm) concentrations of dithiothreitol, propylthiouracil (ptu) inhibited i-5'd in the brain, pituitary, and brown adipose tissue (bat) of hypothyroid rats (which contain predominantly the type ii activity); the patterns of inhibition in these tissues were essentially similar, w ... | 1990 | 2328700 |
| inhibition of 5'-deiodination of thyroxine suppresses the cold-induced increase in brown adipose tissue messenger ribonucleic acid for mitochondrial uncoupling protein without influencing lipoprotein lipase activity. | young adult male and female djungarian hamsters were exposed to ambient temperatures of 23 or 0 c for 12 h; half of the animals in each group were treated with iopanoic acid to suppress the peripheral conversion of t4 to the thermotropically active thyroid hormone t3 by the enzyme 5'-deiodinase (5'd). brown adipose tissue (bat) mrna for uncoupling protein (ucp), bat lipoprotein lipase (lpl) activity, and 5'd activity were measured at the conclusion of the study. a temperature of 0 c produced lar ... | 1990 | 2328697 |
| edrophonium antagonism of atracurium during enflurane anaesthesia. | to determine the influence of enflurane on the ability of edrophonium to antagonize atracurium block, dose-response curves were constructed for edrophonium in the presence of 0%, 1% and 2% enflurane, and for 2% enflurane discontinued at the time of administration of edrophonium. one hundred asa physical status i or ii patients (four groups of 25), selected randomly and undergoing elective surgery, received atracurium 0.5 mg kg-1, with thiopentone, nitrous oxide and enflurane. supplementary doses ... | 1990 | 2328178 |
| novel sulfated polymers as highly potent and selective inhibitors of human immunodeficiency virus replication and giant cell formation. | novel synthetic sulfated polymers, namely, sulfated polyvinyl alcohol (pvas) and sulfated copolymers of acrylic acid with vinyl alcohol (pavas), proved to be potent and selective inhibitors of human immunodeficiency virus type 1 (hiv-1) and hiv-2 in vitro. the compounds completely inhibited hiv-1-induced cytopathogenicity in mt-4 cells and hiv-1 antigen expression in cem cells at a concentration of 0.8 micrograms/ml. they were equally effective against hiv-2 replication. in addition, and in cont ... | 1990 | 2327749 |
| persistent immune deficiency in patients with alcoholic hepatitis. | t-lymphocyte subset numbers, recall-antigen skin test responses and mitogen-induced lymphocyte proliferation assays were investigated in 12 patients with severe alcoholic hepatitis (ah). serial studies of these parameters were obtained at intervals of 1 or 2 wk. patients with ah had lower peripheral blood lymphocyte counts with corresponding decreases in t-cell subset numbers that were persistent in the serial evaluations. t8 cells were disproportionately decreased, with a significant resultant ... | 1990 | 2327386 |
| biphasic response of rat tibial growth to thyroxine administration. | to evaluate the dose-response relationship between thyroxine and tibial growth, 60 male rats age 21 days were rendered hypothyroid by administration of methimazole in the drinking water. twenty-one days later, the hypothyroid rats were randomly divided into 5 groups which received 0, 2, 8, 32, or 64 micrograms.kg-1.day-1 of t4 im for 21 days. all animals were sacrificed at age 64 days. rat tibia were removed for measurement of epiphyseal growth plate width and longitudinal growth rate. serum t4 ... | 1990 | 2327214 |
| [diagnostic significance of low thyrotropin in internal medicine]. | in an attempt to determine the significance of low plasma thyrotropin (tsh) concentrations in internal medicine and the usefulness of systematic tsh assays in hospitals, 732 consecutive tsh measurements were performed in first-admission patients. tsh concentrations below 0.15 mu/l were found in 33 patients (4.5%) divided into 4 groups: a) in 5 patients a second assay made within 10 days of the first one showed no fall in tsh levels; b) 5 patients had known endocrine disease; c) in 8 patients hyp ... | 1990 | 2326556 |
| a mutant t4 lysozyme (val 131----ala) designed to increase thermostability by the reduction of strain within an alpha-helix. | an attempt has been made to identify residues in t4 phage lysozyme that may have strained conformations and, by appropriate site-directed replacements, to reduce this strain and thus increase the thermostability of the protein. valine 131, within alpha-helix 126-134, was identified as a potential candidate. its side-chain rotational angle, chi 1, differs by approximately 18 degrees from the low-energy trans configuration. in addition, it is largely solvent exposed, yet is held in a rigid conform ... | 1990 | 2326253 |
| perturbation of dna hairpins containing the ecori recognition site by hairpin loops of varying size and composition: physical (nmr and uv) and enzymatic (ecori) studies. | we have investigated loop-induced structural perturbation of the stem structure in hairpins d(gaattcxngaattc) (x = a, t and n = 3, 4, 5 and 6) that contain an ecori restriction site in close proximity to the hairpin loop. oligonucleotides containing either a t3 or a a3 loop were not hydrolyzed by the restriction enzyme and also showed only weak binding to ecori in the absence of the cofactor mg2+. in contrast, hairpins with larger loops are hydrolyzed by the enzyme at the scission site next to t ... | 1990 | 2326190 |
| [changes in the plasma levels of t3-t4 and tsh during surgical stress and anesthesia with isoflurane]. | the authors describe the blood level changes of t3, t4 and tsh in 17 patients undergoing urological surgery and isoflurane anaesthesia. the data were statistically processed and showed that isoflurane, used as the only anesthetic agent in 1.5%-2% concentrations, is not sufficient to block hormonal changes of the hypothalamus-hypophysis-thyroid axis caused by surgical stress. | 1990 | 2325871 |
| long-term food restriction depresses serum thyroid hormone concentrations in the rat. | long-term food restriction exerts an anti-aging action in rodents. the mechanism underlying its modulation of aging processes is unknown but changes in endocrine systems have been postulated as couplers of food restriction to aging. the effects of long-term food restriction on the serum concentrations of thyroid hormones were examined in 6-month-old, male fischer 344 rats. for 4.5 months a food restricted group was fed 60% of the amount consumed by an ad libitum group. food restriction did not a ... | 1990 | 2325443 |
| crystallization of hevamine, an enzyme with lysozyme/chitinase activity from hevea brasiliensis latex. | hevamine, an enzyme with both lysozyme and chitinase activity, was isolated and purified from hevea brasiliensis (rubber tree) latex. the enzyme (molecular weight 29,000) is homologous to certain "pathogenesis-related" proteins from plants, but not to hen egg-white or phage t4 lysozyme. to investigate the atomic details of the substrate specificity and the cause for hevamine's low ph optimum (ph 4.0), we have crystallized two hevamine isozymes as a first step towards a high-resolution x-ray stru ... | 1990 | 2325127 |
| in vitro dissolution and in vivo bioavailability of commercial levothyroxine sodium tablets in the hypothyroid dog model. | the objective of this study was to determine whether a correlation exists between the rate of in vitro dissolution and bioavailability of levothyroxine sodium (t4) tablets. dissolution versus time profiles for synthroid, the flint brand of levothyroxine sodium, and two competitors' tablets (brands a and b) were generated using an official dissolution apparatus (usp), and 0.05 m phosphate buffer (ph 7.4) as the medium. these tablets were also utilized in single-dose crossover bioavailability stud ... | 1990 | 2324959 |
| synthesis, metabolic stability and chemotactic activity of peptide t and its analogues. | acquired immunodeficiency syndrome (aids) is initiated by the attachment of the human immunodeficiency virus (hiv) to a surface glycoprotein cd4 present on t4 helper/inducer lymphocytes, monocytes/macrophages and other cells. a simple octapeptide (h-ala-ser-thr-thr-thr-asn-tyr-thr-oh, peptide t) seems to inhibit hiv infectivity and to activate human monocyte chemotaxis. in order to study in vitro metabolic stability and structure-activity relationships, peptide t and a number of analogues were p ... | 1990 | 2323889 |
| cervical epidural anaesthesia for carotid artery surgery. | a series of 394 patients (251 men, 143 women; mean age 70.0 +/- 8.4 yr) selected for carotid artery surgery (cas) performed under cervical epidural anaesthesia (cea) was analysed retrospectively. carotid endarterectomy was performed in 326 patients and saphenous vein bypass in 68. the cervical epidural administration of 15 ml 0.5 per cent bupivacaine or 0.37-0.40 per cent bupivacaine plus fentanyl (50-100 micrograms) resulted in an effective sensory blockade from c2 to t4-t8. patients were maint ... | 1990 | 2322972 |
| correlation between integrated evoked emg and respiratory function following atracurium administration in unanaesthetized humans. | in six healthy volunteers, integrated evoked electromyography (ieemg) measured in response to ulnar nerve stimulation was correlated with respiratory function-tidal volume (vt), forced vital capacity (fvc), forced expiratory volume in one second (fev1), maximum negative inspiratory pressure (nip), hand grip (hg) and head-lift during steady-state infusion of subparalytic doses of atracurium. studies were carried out at train-of-four t4/t1 = 0.2 and t4/t1 = 0.6. when t4/t1 = 0.2, all subjects had ... | 1990 | 2322969 |
| 32p-postlabelling of 7-methyl-dgmp ring-opened 7-methyl-dgmp and platinated dgpdg. | the 32p-postlabelling technique introduced by randerath and coworkers was used to investigate the efficiency of the phosphorylation reaction by t4 polynucleotide kinase using three synthesized adducts: 7-methyl-dgmp, ring-opened 7-methyl-dgmp and platinated dgpdg. the methylated substrates were detected at sub-fmol sensitivities. 7-methyl-dgmp was quantitatively phosphorylated at these low concentrations. the efficiency of phosphorylation of the ring-opened product was less (about one order of m ... | 1990 | 2322952 |
| cognition in early human immunodeficiency virus infection. | relatively little is known about cognitive changes in early human immunodeficiency virus (hiv) infection. this study examined cognitive functioning in 46 hiv-positive gay men relative to an age and education equivalent group of 13 hiv-negative gay men. the hiv-positive men were asymptomatic except for lymphadenopathy or t4 counts less than 700. the cognitive battery measured language, memory, visuospatial, information processing speeds, reasoning, attention, and psychomotor processes. the hiv-po ... | 1990 | 2322137 |
| stimulation with submaximal current for train-of-four monitoring. | the present study evaluated responses to train-of-four (tof) stimulation at a range of stimulating currents. traditionally tof has been applied with a supramaximal stimulus but this may be quite uncomfortable for the awake patient. in the first part of this study, 12 healthy volunteers quantified (by 10-cm visual analog scale) the discomfort associated with tof stimulation at 20, 30, and 50 ma. the median vas scores were 2, 3, and 6, respectively (p less than 0.05 for differences between each gr ... | 1990 | 2321779 |
| breast cancer screening in an urban population in northern italy. | a mammographic and clinical screening for breast cancer started in june 1987 in the health district of brescia, northern italy, including the town and 23 surrounding municipalities. this paper describes the organization and the results of the first 12 months of screening. of 7791 invited women aged 50-60 years, 5217 (67%) agreed to participate. there was a trend for response rates to decline with increasing age and education. of the 5217 women examined, 66 (1.3%) were referred for biopsy and 64 ... | 1990 | 2321269 |
| application of the method of phage t4 dna ligase-catalyzed ring-closure to the study of dna structure. ii. nacl-dependence of dna flexibility and helical repeat. | in this work, we demonstrate that it is possible to determine the molar cyclization factor jm from single ligation reactions in which both circular and linear dimer dna species are formed concurrently from linear monomers. this approach represents a significant improvement over previous methods, in which jm is evaluated from the ratio of the rate constants for two separate processes; namely (1) the cyclization of linear dna and (2) the association of two linear molecules to form linear dimers. d ... | 1990 | 2319604 |
| application of the method of phage t4 dna ligase-catalyzed ring-closure to the study of dna structure. i. computational analysis. | the tendency for relatively short (less than 500 base-pair) dna molecules to circularize in the presence of dna ligase is a sensitive function of both the lateral and torsional flexibilities of the molecules being studied. this tendency is reflected in a quantity known as the j-factor, which is determined experimentally by measuring the relative rates of circle and linear dimer formation at a specified concentration of linear monomer. shimada & yamakawa have provided an analytical representation ... | 1990 | 2319603 |
| t4-thyroid storm after ct-scan with iodinated contrast medium. | a 62-year-old woman had thyroid storm 5 h after a ct examination (contrast enhancement with 65% meglumine amidotrizoate, angiografin). at the time of admission to our hospital, serum t4 and serum free t4 levels were markedly elevated (29 micrograms/dl and 9.6 ng/dl, respectively); serum t3 and serum free t3 levels were within normal limits (144 ng/dl and 5.9 pg/ml, respectively). these findings suggest that thyroid storm can occur with excess t4 only (t4-thyroid storm), and that t4-thyroid storm ... | 1990 | 2319111 |
| stimulation of liver mitochondrial alpha-glycerophosphate dehydrogenase activity by l-thyroxine in thyroidectomized rats: comparison with the suppression of pituitary tsh secretion. | the rate of liver mitochondrial alpha-glycerophosphate dehydrogenase (gpd) induction was compared to the suppression of pituitary thyrotropin (tsh) secretion in thyroidectomized rats submitted to prolonged administration of small amounts of l-thyroxine (t4). with both 350 and 530 ng t4/100 g bw/day, liver alpha-gpd activity remained at post-thyroidectomy level (mean +/- se: 0.030 +/- 0.002 and 0.034 +/- 0.001 delta a/mg prot/min, respectively) throughout all experiment. a sharp increase in enzym ... | 1990 | 2319108 |
| changes in serum triiodothyronine (t3) kinetics after prolonged antarctic residence: the polar t3 syndrome. | humans who live in antarctica for greater than 5 continuous months demonstrate alterations in the hypothalamic-pituitary-thyroid axis. these changes are characterized by 1) increased pituitary release of tsh in response to iv trh, 2) increased serum clearance of orally administered t3, and 3) normal serum total, free t4, and unstimulated tsh levels. to clarify the mechanism responsible for these findings, serum kinetic studies of 125i-labeled t4 and t3 were carried out in a group of normal men, ... | 1990 | 2318952 |
| androstenedione and estrone dynamics in hypothyroid women. | to examine the effects of hypothyroidism on androstenedione (a) and estrone (e1) metabolism, we infused eight women with [3h]androstenedione and [14c]estrone when they were hypothyroid and again when they were euthyroid after levo-t4 administration. mcrs and conversion ratios were measured using concentrations of radioactivity in the blood pools of a and e1. peripheral aromatization was measured from the concentrations of radioactivity in the e1 glucuronide pool in the urine. after the women wer ... | 1990 | 2318947 |
| effects of l-thyroxine and iodide on the development of autoimmune postpartum thyroiditis. | in the present study we examined the influence of l-t4 and iodide on autoimmune postpartum thyroiditis. women at risk of developing the disease were identified in early pregnancy by the presence of moderate or high titers of antibodies against thyroid peroxidase (tpoab). they were given no treatment (n = 20), 0.1 mg l-t4 daily (n = 18), or 0.15 mg iodide daily (n = 20) for 40 weeks postpartum. changes in thyroid function were seen in all women, although in nine hormone values remained within the ... | 1990 | 2318931 |
| affinity labeling of rat liver and kidney type i 5'-deiodinase. identification of the 27-kda substrate binding subunit. | extrathyroidal production of 3,3',5-triiodothyronine from the thyroid secretory product, thyroxine, is catalyzed by tissue-specific iodothyronine 5'-deiodinases. type i 5'-deiodinase (5'd-i) produces greater than 75% of the t3 found in the circulation and in thyroid hormone-responsive tissues and is most abundant in rat liver and kidney. in this study, we used the bromoacetyl derivatives of t4 (n-bromoacetyl-[125i]l-thyroxine, bract4) and t3 (n-bromoacetyl-[125i]3,3',5-triiodothyronine, bract3) ... | 1990 | 2318852 |
| apolipoprotein a-iv messenger ribonucleic acid abundance is regulated in a tissue-specific manner. | the influence of development and estrogen, thyroid hormone, corticosteroid, and fibrate administration on apolipoprotein (apo) a-iv mrna levels in the liver and intestine and on serum or plasma concentrations of apo a-iv was studied in the rat. treatment of ovariectomized rats with ethinyl estradiol provoked a dose-dependent decrease in liver apo a-iv mrna levels, whereas intestinal apo a-iv mrna did not change. the serum apo a-iv concentration decreased in a dose-dependent manner. administratio ... | 1990 | 2318160 |
| two pathways for thyroxine 5'-monodeiodination in brown adipose tissue in fetal sheep: ontogenesis and divergent responses to hypothyroidism and 3,5,3'-triiodothyronine replacement. | thermogenesis in rat brown adipose tissue (bat) is thyroid hormone responsive. rat bat expresses a type ii 5'-iodothyronine monodeiodinase (5'mdi) which mediates local t3 production from t4. earlier studies show that bat from fetal and newborn sheep contains a high km type i, instead of type ii, 5'mdi. to better characterize the 5'mdi of ovine fetal bat, we studied the in vitro monodeiodination of [125i]t4 at a low substrate concentration (2 nm) and in the presence of 1 mm propylthiouracil in ba ... | 1990 | 2318152 |
| [treatment of cancer of the anal canal with combination radiochemotherapy: apropos of 12 cases]. | we present 12 patients (3 male, 9 female) aged from 53-88 years with squamous cell cancer of the anal canal treated with combined radiotherapy-chemotherapy (5 fu-mitomycin c); the distribution of the tumours was 2 t4, 6 t3, 3 t2, 1 t1). in 11 patients evaluated, complete remission was obtained in 8 with partial remission in 2 others. in spite of this good result with combined treatment, 4 patients had a recurrence between 9 and 26 months. five patients survived and 2 died without disease (16-38 ... | 1990 | 2317581 |
| [role of preoperative radiotherapy in preservation of the anal sphincter in cancer of the lower rectum]. | short term preoperative irradiation of rectal cancer (30 gy in 12 days) followed by a two-month rest before surgery has proven to be more efficient than most usual protocols of radiotherapy. in a series of 136 tumors t2, t3 or t4, tumor-free specimens and duke'a lesions were found at surgery in 17% and 36% respectively. surgeons took advantage of the tumor regression and performed sphincter-saving surgery in 41 patients with t2, t3 or t4 tumors of the lower third of the rectum. of 30 patients fo ... | 1990 | 2317572 |
| patterns of cervical node metastases from squamous carcinoma of the larynx. | we undertook a retrospective review of 247 previously untreated consecutive patients from 1965 to 1986 with primary squamous cell carcinoma of the supraglottic or glottic larynx to ascertain the prevalence of neck node metastases by neck level. the 247 patients underwent a total of 262 radical neck dissections. patients were grouped by clinical neck status at the time of neck dissection: elective dissection in the no neck; immediate therapeutic dissection in the n+ neck; and subsequent therapeut ... | 1990 | 2317325 |
| a strategy for the construction of double-stranded dna microcircles with circumferences less than 50 bp. | a procedure for the construction of double-stranded dna microcircles is described that overcomes the natural limits of established circularization procedures. the assembly strategy needs only three components, i.e. two synthetic oligonucleotides containing any sequence of interest and t4 dna ligase. all components can be provided in large quantities and only two enzymatic reaction steps are required. such microcircles can serve as a model for topologically fixed and highly bent dna molecules and ... | 1990 | 2317253 |
| effects of age, sex, and body size on serum concentrations of thyroid and adrenocortical hormones in dogs. | thyroxine (t4), 3,5,3'-triiodothyronine (t3), and cortisol frequently are quantified in canine serum or plasma samples to aid in the diagnosis of hypothyroidism, hypoadrenocorticism, and hyperadrenocorticism. many laboratories have established reliable references values for concentrations of these hormones in blood of clinically normal animals. however, nonpathologic factors that affect thyroidal and adrenocortical secretion may lead to misinterpretation of test results when values for individua ... | 1990 | 2316925 |
| intraoperative and external beam radiotherapy in invasive bladder cancer: pathological findings following cystectomy. | the pathological findings observed following intraoperative radiotherapy (iort) boost (15gy) to the whole bladder, external beam fractionated irradiation (46gy in 5 weeks), and planned radical cystectomy in patients with deep invasive bladder carcinoma are analyzed. clinical pretreatment stage of disease was t3 (16 cases) and t4 (two cases). no evidence of residual tumor (pt0) was demonstrated in 11 cystectomy specimens (61%) and residual tumor (pt+) was observed in seven (39%). toxicity and com ... | 1990 | 2316479 |
| effect of amiodarone on non-deiodinative pathway of thyroid hormone metabolism. | amiodarone, an iodine containing anti-arrhythmic drug, causes a significant decrease in molar ratio of daily production rates of t3 and t4 from 0.75 in controls to 0.36 in amiodarone-treated rabbits. a model was constructed from the above data which showed that metabolism of t4 via non-deiodinative pathways (e.g. tetraiodothyroacetic acid and/or conjugates) increased from 29% in untreated controls to 66% in amiodarone-treated rabbits. in this study, we have examined the metabolic clearance rate ... | 1990 | 2316313 |
| the effect of fasting on thyroidal t4-5' monodeiodinating activity in mice. | complete fasting induces a significant decrease of serum t3 and a fall in tsh in rodents and man. to evaluate the effect of starvation on thyroidal t4 5'monodeiodinating activity, in vitro conversion of t4 to t3 by thyroid and liver homogenate from one to three days fasted mice was compared with homogenates from control mice on animal chow. 5'monodeiodinating activity was significantly lower in thyroid homogenates of fasted mice than in those of chow-fed control [100 +/- 5.0 and 92 +/- 5.0 pmol ... | 1990 | 2316308 |
| hormonal and cardiorespiratory changes following simulated saturation dives to 4 and 11 ata. | professional divers were compressed with trimix to 4 ata (2 persons, aged 35 and 26) and to 11 ata (3 persons, aged 34, 26, and 23) for saturation dives with durations of 48 and 50 h, followed by 33 and 109 h of decompression, respectively. pre- and postdive cardiorespiratory reactions to a step test--heart rate (hr) and ventilation (ve)--and concentrations of growth hormone, corticotropin, cortisol, insulin, lutotropin, folitropin, triiodothyronine (t3), thyroxine (t4), thyrotropin, and testost ... | 1990 | 2316058 |
| [the differential diagnosis of thyroid cancer using radioimmunologic analysis]. | clinical and radioimmunoassay investigations performed in 162 patients with different thyroid diseases, have shown that changes in the levels of t3, t4 and tsh do not allow assessment of the nature of a tumor process. the level of calcitonin is a tumor marker of medullary carcinoma and c-cell thyroid adenomas. in the absence of pathological changes in the thyroid an increase in the level of thyroglobulin can be regarded as a tumor marker of metastatic growth of papillary and papillary-follicular ... | 1990 | 2314197 |