Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [hiv infection and t-lymphocyte behavior: results of a study in palermo]. | the acquired immunodeficiency syndrome (aids) is caused by the hiv infection in people with an already impaired immunological system. the seroprevalence follow-up of different markers of hiv should seem to suggest to predict the course of the disease as well as the t4/t8 ratio did. we examined 111 at risk anti-hiv positive people according to these parameters in comparison with their clinical situation. serum samples from these subjects were examined by means of the elisa method for anti-env/cor ... | 1989 | 2483080 |
| [neo-adjuvant chemotherapy of nasopharyngeal carcinoma]. | 11 patients with advanced nasopharyngeal carcinoma were treated by combination of radiotherapy plus neo-adjuvant chemotherapy using cddp and pep. 1. the primary tumor was located at postero-superior wall of the nasopharynx in 6 cases and at lateral wall in 5 cases. 2. according to tnm classification, the tumor was classified as t1 in 1 case, t2 in 4 cases, t3 in 3 cases and t4 in 3 cases. according to stage grouping, the tumor was grouped as stage iii in 2 cases and stage iv in 9 cases. 3. clini ... | 1989 | 2482880 |
| hyperthyroidism with an fsh-and tsh-secreting pituitary adenoma. | a 34-year-old man was found to have elevated thyroxine (t4), triiodothyronine (t3), calculated free t4, thyroid-stimulating hormone (tsh), follicle-stimulating hormone (fsh), and alpha subunits of tsh and fsh. a computed tomography scan of the head showed a 16-mm macroadenoma of the pituitary gland. there was no evidence of loss or excess secretion of other pituitary hormones. the large chromophobe adenoma was removed via a transphenoidal approach. the patient has been taken off all medication. ... | 1989 | 2482837 |
| [natural cytotoxic function and ankylosing spondylitis]. | natural killer (nk) cell function was evaluated in 28 ankylosing spondylitis (as) patients (21 b27 positive), at one hand by the use of a leu7 (hnk-1) monoclonal antibody, at the other hand by spontaneous cytotoxicity against a k562 cell line (preincubated with a fluorogenic substrate) evaluated by a flow cytometric assay. there is no difference in nk cell activity neither between as patients and controls nor between b27 positive and negative as. the study brings no evidence for a nk function co ... | 1989 | 2482475 |
| specificity of the binding of bacteriophage m13 encoded gene-5 protein to dna and rna studied by means of fluorescence titrations. | the fluorescence quenching of the bacteriophage m13 encoded gene-5 protein was used to study its binding characteristics to different polynucleotides. experiments were performed at different salt concentrations and in some instances at different temperatures. the affinity of the protein depends on the base and sugar composition of the polynucleotides involved and may differ appreciably, i.e. by orders of magnitude. the salt dependence of binding is within experimental accuracy equal for all sing ... | 1985 | 2482044 |
| rat heart thyroxine 5'-deiodinase is sensitively depressed by amiodarone. | thyroxine 5'-deiodinase activity (5'-d) is depressed in rat liver by amiodarone. to investigate the possible tardive effect of amiodarone on 5'-d in different tissues, rats were fed amiodarone according to a short-term (group a, 50 mg amiodarone/kg/day for 10 days, total dose 150 mg, n = 16) and long-term regimen (group b, 25 mg amiodarone/kg/day for 20 days, n = 16) (control group, n = 12). serum, heart, and other tissues were collected for 10 days after drug cessation. thyroxine (t4), triiodot ... | 1989 | 2481769 |
| mechanistic investigation of species-specific thyroid lesions induced by treatment with the histamine h1 antagonist temelastine (sk&f 93944) in rats. | temelastine (sk&f 93944), an h1 histamine receptor antagonist, induces thyroid histopathological lesions in the rat, indicative of thyroid follicular cell stimulation, at oral doses of 10-33 mg/kg body weight/day. these changes do not occur in the dog or mouse. endocrinological short-term studies support an increased thyroid follicular activity (increased radioiodide accumulation) with decreased circulating thyroxine (t4) at oral doses at or above 300 mg/kg body weight/day and increased circulat ... | 1989 | 2481636 |
| antithyroid drugs in the treatment of hyperthyroidism of graves' disease: long-term follow-up of 434 patients. scottish automated follow-up register group. | a study of antithyroid drug (atd) therapy with a mean follow-up period of 10 years (range 2-25) in 434 patients with graves' disease has been made by linking hospital records with those of a central follow-up register. the majority (89%) were treated with carbimazole and 87% received combined therapy with triiodothyronine (t3) (73%) or thyroxine (t4) (14%). sixty-one per cent were assessed for t3 suppression tests on completion of treatment, of whom 61% (95% cl, 55-67%) suppressed. the overall 5 ... | 1989 | 2481576 |
| [effects of hypoxia on the functional state of the thyroid gland in premature infants of different gestational age]. | altogether 98 premature children of different gestation age were examined for the content of t3, t4 and tth in serum of funic and venous blood on days 3, 5-7 and 30 and part of the children on day 60 of life. all the children were diagnosed to have hypothyroid condition, whose depth and duration were determined by gestation age, by the degree of hypoxia and gravity of the infectious process. with a purpose of the replacement therapy, 12 children were administered thyroidin in a dose of 5 microgr ... | 1989 | 2481255 |
| expression of alpha-fetoprotein receptors by human t-lymphocytes during blastic transformation. | alpha-fetoprotein (afp) and transferrin (tf) are actively internalized by many growing cells during ontogenic and neoplastic development, including human malignant t- and b-lymphoblastoid cells. their internalization is, on the contrary, greatly diminished or absent in mature, non-proliferating elements. in the present work, peripheral blood mononuclear cells (pbmcs) and t-lymphocytes, harvested from normal human donors, were induced to blastic transformation with phytohemagglutinin (pha) and th ... | 1989 | 2481232 |
| [a case of henoch-schoenlein purpura with extremely low okt4 positive t lymphocytes]. | we present a patient with henoch-schoenlein purpura who had extremely low number of okt4 positive t lymphocytes. however his lymphocytes responded normally to leu3a and coulter t4, which are also monoclonal antibodies that react with cd4 epitope. in vitro lymphocyte function tests revealed that helper-inducer t cell functions were normal. from these findings, we concluded that this patient had an abnormality in the epitope of cd4 positive cells. since expression of okt4 antigens in his mother wa ... | 1989 | 2481051 |
| endocrine effects of blinding in male syrian hamsters are associated with increased hypothalamic 5-hydroxyindoleacetic acid/serotonin ratios. | the antigonadal, antithyroid, and antiadrenal effects of blinding were studied in male syrian hamsters receiving propylthiouracil (ptu) or ptu plus thyroxine (t4) replacement. ten weeks after blinding, the expected gonadal involution and reduction of circulating levels of t4, as well as a reduction of circulating levels of corticosterone, were observed. t4 treatment significantly increased testicular weights, but it did not prevent gonadal involution. neither ptu nor t4 administration significan ... | 1989 | 2481027 |
| immunoreconstitution after human bone marrow transplantation. | immunologic reconstitution was studied in 24 patients who underwent bone marrow transplantation, 17 allogenic and 7 autologous. the gvhd prophylaxis consisted of methotrexate and prednisone. the complete immune evaluation was to be carried out prior to transplantation at 1, 2, 3, 6, 9, 12 months after bmt and subsequently every 6 months up to 4 years. the investigated immunological parameters included total lymphocyte count, b-lymphocytes, t3-, t4-, t8-lymphocytes, t4/t8 ratio, natural killer ce ... | 1989 | 2480301 |
| two different mechanisms are involved for the bleomycin-induced dna repair synthesis in permeabilized hela cells. | bleomycin-induced dna repair synthesis in the permeabilized hela cells was sensitive to aphidicolin, an inhibitor of dna polymerase alpha and delta, and to dideoxythymidine triphosphate (ddttp), a specific inhibitor of dna polymerase beta. upon combined treatment with these inhibitors, the dna repair synthesis was inhibited to an even higher degree. this indicated that the aphidicolin- and ddttp-sensitive dna repair syntheses may occur by independent mechanisms. the structure of incomplete repai ... | 1989 | 2480115 |
| evidence for immunological differences between circulating and thyroid tissue-derived thyroglobulin in men. | the expression of six antigenic epitopes on plasma tg and tissue-derived tg was investigated using mouse mabs in immunoradiometric assays. the sensitivity of the assays ranged between 0.5 and 1.5 micrograms l-1. plasma thyroglobulin of 15 normal individuals and 18 patients with metastatic, differentiated thyroid cancer was analysed. whereas five monoclonals produced values comparable to the conventional ria system, the monoclonal antibody tg 158 did not detect any tg in the normal individuals an ... | 1989 | 2479559 |
| possible cofactors in the etiology of cervical intraepithelial neoplasia. an immunopathologic study. | previous work in our department demonstrated a reduction in the numbers of langerhans' cells in cervical epithelium showing histologic changes of human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin). in conjunction with a localized reversal of the t4:t8 ratio of t-lymphocytes, that finding provides evidence of the association of epithelial immunosuppression with both hpv infection and cin. to investigate whether that phenomenon occurs primarily because of hpv alone o ... | 1989 | 2478707 |
| an evaluation of morphine and oxymorphone administered via patient-controlled analgesia (pca) or pca plus basal infusion in postcesarean-delivery patients. | the analgesic efficacy and adverse effects of morphine and oxymorphone in 32 patients who received traditional patient-controlled analgesia (pca) following cesarean delivery were compared with those in 32 other patients receiving the same agents via pca plus basal opioid infusion (pca + bi). all patients were operated upon during epidural anesthesia with 2% lidocaine and 1:200,000 epinephrine to achieve a t4 sensory level. upon first complaint of pain in the recovery room, patients were given a ... | 1989 | 2478049 |
| csf t-cell subsets in multiple sclerosis: relationship to cerebrospinal fluid myelin basic protein and clinical activity. | cerebrospinal fluid myelin basic protein and cerebrospinal fluid and peripheral blood t-cell subsets have been studied in patients with multiple sclerosis and other inflammatory and non-inflammatory nervous system diseases. these biological parameters have been correlated with clinical disease activity. no changes in peripheral blood t-cell subsets were seen in multiple sclerosis patients. low cerebrospinal fluid t8+ cells occurred only in multiple sclerosis, while high cerebrospinal fluid t4+ c ... | 1989 | 2477508 |
| effects of l-thyroxine and l-triiodothyronine on protein and nucleic acid contents of liver of 6n-2-propylthiouracil treated hypothyroid singi fish, heteropneustes fossilis bloch. | three consecutive injections of 12.5 x 10(-10) and 25 x 10(-10) moles/g of l-thyroxine (t4) or a single injection of l-triiodothyronine (t3) at 7.5 x 10(-10) moles/g to singi fish caused an increase in liver protein and rna contents, whereas similar injections of 50 x 10(-10) moles/g of t4 or 75 x 10(-10) moles/g of t3 caused a fall in these cellular constituents in liver. treatments of singi fish with thiourea (1 mg/ml) for 30 days caused a fall in the protein and rna contents in liver which we ... | 1989 | 2477322 |
| birth weight and thyroxine-binding globulin in pre-eclampsia. | plasma concentrations of thyroxine (t4) and thyroxine-binding globulin (tbg) as well as triiodothyronine uptake (t3u) were measured in 32 proteinuric pre-eclamptic patients and 24 normotensive pregnant women at similar gestations in the third trimester. the pre-eclamptic patients had slightly lower tbg, and significantly lower t4, concentrations, which were significantly correlated. they also had significantly lower infant birth weight which was correlated to the tbg and t3u values. there was no ... | 1989 | 2476368 |
| quality of life in patients with muscle-infiltrating bladder cancer and hormone-resistant prostatic cancer. | a questionnaire, filled in by the patient, was used to assess the quality of life of 65 patients with previously untreated t2-t4 nx mo bladder cancer and of 67 patients with hormone-resistant prostatic cancer. this study examines the initial questionnaire filled in before any nonsurgical treatment had been started in the patients referred to an oncological ward. the aim was to identify domains in which distress was frequent, in the hope of indicating where treatment directed forward improving th ... | 1989 | 2476317 |
| the management of hyperthyroidism due to graves' disease in japan in 1988. the japan thyroid association. | the management of hyperthyroidism due to graves' disease in japan was the subject of a survey of the members of the japan thyroid association (jta), and the results were compared to those of the european thyroid association (eta). in the questionnaire, in vivo and in vitro diagnostic procedures, the choice of treatment and the details of the treatment for a patient with typical, moderate and uncomplicated hyperthyroidism due to graves' disease was at first asked, and eight variations with a sing ... | 1989 | 2476301 |
| tumor and serum beta-2-microglobulin expression in women with breast cancer. | to investigate whether the tumor expression of beta-2-microglobulin (beta 2-m) could serve as a marker of tumor biologic behavior, the authors studied specimens of breast carcinomas from 60 consecutive female patients. presence of beta 2-m was analyzed by immunohistochemistry. no significant correlations were found between tumor beta 2-m expression and several histologic attributes such as type, histologic and nuclear grades, mitotic index, necrosis, vascular invasion, and lymphocytic infiltrati ... | 1989 | 2476028 |
| role of beta adrenoceptors in the hypertrophic response to thyroxine. | the ability of beta-adrenoceptor blockade to reduce the hypertrophic response to thyroxine (t4, 0.5 mg/kg per day, s.c.) was tested in new zealand white rabbits. two beta-adrenergic blocking agents, one a full antagonist (propranolol, 9.6 mg/kg per day) and the other a partial agonist (pindolol, 0.96 mg/kg per day) were administered in combination with t4 in an effort to reduce myocardial hypertrophy. a 3 and 16 day group were generated to test the time course of the hypertrophic and receptor re ... | 1989 | 2475716 |
| [molecular cloning of butirosin biosynthesis genes. i. construction and characterization of recombinant plasmid dna no. 2 and no. 11]. | b. circulans nrrl-b 3312 is a butirosin-producing strain, butirosin being one of aminoglycoside antibiotics. we used shotgun strategy for molecular cloning of butirosin biosynthesis genes. ecori digests of b. circulans nrrl-b 3312 chromosome dna were ligated to ecori digests of plasmid pub110 with t4-dna ligase. recombinant dna was transformed into competent cells of b. subtilis 168. kanr transformants were selected on medium containing kanamycin (5 micrograms/ml). results showed that kanamycin ... | 1989 | 2475268 |
| cartilage response to thyroid hormones in the sex-linked dwarf chicken. | 1. trachea cartilages were dissected from normal and dwarf chickens which had been injected with thyroxine (t4, 200 micrograms/kg) or triiodothyronine (t3, 200 micrograms/kg) for seven consecutive days, and were analysed for nucleic acids, proteins and polyamines. 2. in saline-injected control chickens, rna, but not dna and protein, concentration of the cartilage was higher in dwarfs than in normals. the concentration of putrescine was lower in dwarfs than in normals, while that of spermine was ... | 1989 | 2474410 |
| polyclonal rabbit gamma globulins against a human cytotoxic cd4 t cell clone. ii. use in prevention of rejection in kidney transplantation: a pilot study. | antiblast globulins (gab) were prepared by immunization of rabbits with activated t lymphocytes (at) derived from a rejected kidney allograft. at consisted of a cd4+ (cd3+, cd2+ tcr alpha+ beta+) clone cytotoxic for hla dr8-positive targets. the immunizing cells were adapted to industrial growth conditions by repetitive stimulations with an ebv-transformed line from the kidney donor and recombinant il-2. in the pilot study, gab (1.0-1.5 mg/kg/day) was given in 12-hr infusions, in association wit ... | 1989 | 2474211 |
| serum antibody pattern, antigenemia, and virus isolation in infants born to mothers seropositive for human immunodeficiency virus type 1. | forty children born to mothers seropositive for the human immunodeficiency virus type 1 (hiv-1) followed-up to 15 months after birth were studied by means of serum antibody patterns to individual viral polypeptides, by the presence of detectable levels of viral core antigen (p24) and virus in serum and peripheral blood lymphocytes, and by total lymphocyte counts and t4/t8 lymphocyte ratios. the results obtained indicate that a persistent antigenemia is significantly associated with positive viru ... | 1989 | 2474055 |
| encapsulation of the uv-dna repair enzyme t4 endonuclease v in liposomes and delivery to human cells. | t4 endonuclease v, a pyrimidine-dimer-specific dna repair enzyme, was encapsulated in liposomes under mild conditions. the encapsulated enzyme was active, and when applied to ultraviolet (uv)-irradiated human cells in culture, the liposomes increased incision of uv-irradiated cellular dna, enhanced dna repair replication, and enhanced survival of uv-irradiated cells. this method is a first step in a new approach for topical application of dna repair enzymes to human skin to prevent skin cancer. | 1989 | 2474027 |
| pediatric nasopharyngeal carcinoma: long term follow-up of 29 patients. | twenty-nine untreated children diagnosed with nasopharyngeal carcinoma were consecutively admitted to st. jude children's research hospital from 1962 to 1986. the age of the patients ranged from 6 to 19 years (median of 13) at diagnosis. histologically, all had lymphoepithelioma. patients were retrospectively staged in the american joint committee system. disease extent was t1 (n = 5), t2 (n = 7), t3 (n = 9), t4 (n = 8); n0 (n = 1), n2 (n = 7), n3 (n = 21). two patients had distant metastasis (m ... | 1989 | 2473970 |
| autologous transplantation of bone marrow purged in vitro with anti-cd7-(wt1-) ricin a immunotoxin in t-cell lymphoblastic leukemia and lymphoma. | seven patients with high-risk acute t-cell lymphoblastic leukemia (t-all) and six with t cell lymphoma (t-ll) were treated with autologous bone marrow transplantation (abmt) after in vitro purging of their bone marrow with wt1 (cd7)-ricin a-chain immunotoxin. cd7 expression on the tumor cells showed large variations between the individual patients and was highly related to the specific cytotoxicity of wt1-ricin a. incubation of bone marrow with up to 10(-8)mol/l wt1-ricin a in the presence of 6 ... | 1989 | 2473811 |
| replication of hiv in human fetal retinal cultures and established pigment epithelial cell lines. | the ability of the human immunodeficiency virus type 1 (hiv-1) to replicate in cells derived from ocular tissue was studied. primary retinal cultures (containing both glial and neuronal cells) were found to support the replication of hiv upon transfection with molecularly cloned proviral dna. in addition, established retinal pigment epithelial (rpe) cell lines also produced hiv particles upon transfection. hiv released by these cell lines was able to infect and induce characteristic cytopathic e ... | 1989 | 2473046 |
| a putative homoeolog of human chromosome 12 in the owl monkey. | analyses of southern blots of rodent x owl monkey somatic cell hybrids permitted syntenic assignment of gene loci coding for triosephosphate isomerase (tpi), antigen cd4(t4), kirsten rat sarcoma 2(kras2) virus, insulin-like growth factor 1 (igf1), and alpha 2-macroglobulin (a2m) to chromosome 10 of owl monkey karyotype vi(2n = 49, 50). in addition, regional in situ localization of the t4 and kras2 loci on the proximal region of the long arm of this acrocentric chromosome and on the corresponding ... | 1989 | 2472934 |
| pulmonary kaposi's sarcoma: clinical findings and results of therapy. | kaposi's sarcoma occurs commonly in patients with the acquired immunodeficiency syndrome (aids). kaposi's sarcoma may remain localized or disseminate to involve visceral organs such as the lungs. disseminated pulmonary involvement, when it occurs, is often fatal. effective therapy may improve survival in such patients. we herein report on 20 patients with disseminated pulmonary kaposi's sarcoma treated with cytotoxic chemotherapy. | 1989 | 2472743 |
| [clinicopathological study of advanced cancers of the oral cavity and pharynx--evaluation of the effects of chemotherapy and radiotherapy]. | some advanced oral cavity and pharynx cancer patients in stage t3 or t4 have been found to be therapeutically uncontrollable even by combination radio-and chemotherapy. therefore, after preoperative treatment, operations were performed which effected reduction and scaring of the tumor that mimicked healing. we then made step-serial sections from the excised materials and analyzed the invasive depth of the cancer cells and the efficiency of therapy three-dimensionally by mapping the distribution ... | 1989 | 2472497 |
| evidence for neuropeptide y synthesis in the rat anterior pituitary and the influence of thyroid hormone status: comparison with vasoactive intestinal peptide, substance p, and neurotensin. | neuropeptide y (npy), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by ria and immunocytochemistry in the rat anterior pituitary gland. npy prohormone messenger rna (mrna) was identified in the pituitary by northern blot analysis. the possible regulation of npy was examined by determining the effects of thyroid hormone manipulation on peptide synthesis. three other anterior pituitary neuropeptides, neurotensin (nt), substance p (sp), and vasoactive intestin ... | 1989 | 2472269 |
| [locally advanced cardia carcinoma--is resection worthwhile?]. | long-term results in 217 patients with carcinoma of the cardia were analysed prospectively. 43 of the 217 could not be operated upon, either because of their poor general condition (51%), distal metastases (49%), and (or) the primary tumour having advanced too far locally (9%). two of the 176 patients proved to be inoperable at surgery so that only 174 underwent resection (operation rate 81%; resection rate 99%). the operation risk was no higher for advanced tumour (t3/t4) than for earlier tumou ... | 1989 | 2472258 |
| elucidation of the structure of the pasteurella haemolytica serotype t10 lipopolysaccharide o-antigen by n.m.r. spectroscopy. | the aqueous-phase lipopolysaccharide isolated from pasteurella haemolytica serotype t10 cells by the phenol-water extraction method was found to be s-type lipopolysaccharide which possessed o-antigenic polysaccharide chains composed only of d-galactose residues. structural analysis of the o-polysaccharide, using a combination of 1d and 2d 1h- and 13c-n.m.r. methods, led to the identification of the disaccharide repeating-unit as [----3)-alpha-d-galp-(1----3)-beta-d-galf-(1----]n. the serological ... | 1989 | 2472201 |
| persistence of abnormal chloride conductance regulation in transformed cystic fibrosis epithelia. | an airway epithelial cell line (cf/t43) was developed by infecting cultured airway epithelial cells from patients with cystic fibrosis (cf) with the pzipneosv(x)1/sv40t retrovirus and selecting for g418 resistance and ion transport properties. the distinctive chloride secretory phenotypes of the cf cell line cf/t43 and a normal cell line (nl/t4) were not perturbed by sv40t-induced cell transformation. epithelial cell lines generated from cf cells with the sv40t gene can be used to test candidate ... | 1989 | 2472008 |
| bacteriophage-t4 and micrococcus luteus uv endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts. | bacteriophage-t4 uv endonuclease nicks the c(3')-o-p bond 3' to ap (apurinic or apyrimidinic) sites by a beta-elimination reaction. the breakage of this bond is sometimes followed by the nicking of the c(5')-o-p bond 5' to the ap site, leaving a 3'-phosphate end; delta-elimination is proposed as a mechanism to explain this second reaction. the ap site formed when this enzyme acts on a pyrimidine dimer in a polynucleotide chain undergoes the same nicking reactions. micrococcus luteus uv endonucle ... | 1989 | 2471512 |
| a cell-free system for studying a priming factor involved in repair of bleomycin-damaged dna. | a simple cell-free system for studying a priming factor involved in the repair of bleomycin-damaged dna was established. the template-primer used for the repair dna synthesis was prepared by treating the closed circular, superhelical form of puc19 plasmid dna with 2.2 microm bleomycin and 20 microm ferrous ions. single-strand breaks were introduced into puc19 dna by the bleomycin treatment, and the dna was consequently converted largely into the open circular form. a system for repair of this bl ... | 1989 | 2471391 |
| an apali restriction site polymorphism is associated with the mb19 polymorphism in apolipoprotein b. | an apolipoprotein (apo) b-specific monoclonal antibody, mb19, detects a commonly occurring two-allele genetic polymorphism in human apob (young, s. g., s. j. bertics, l. k. curtiss, d. c. casal, and j. l. witztum. 1986. proc. natl. acad. sci. usa. 83: 1101-1105). antibody mb19 binds to two different allotypes of apob, mb19(1) and mb19(2), with high and low affinity, respectively. the epitope for antibody mb19 is located within apob-100 thrombolytic fragment t4 (apob-100 amino acid residues 1-129 ... | 1989 | 2470848 |
| effect of a subdural air collection on the sensory evoked potential during surgery in the sitting position. | fifty-three cases with posterior fossa surgery were monitored using sensory evoked potentials (seps) and auditory evoked potentials (aeps) simultaneously. nine of them operated in the sitting position showed typical sep changes during intracranial manipulations that were not attributable either to surgical or to anaesthetic handling. the sep amplitude rapidly recovered when the patients were laid supine after operation. skull x rays revealed intracranial air collections beneath the recording ele ... | 1989 | 2470575 |
| involvement of ion channels in the transport of phage dna through the cytoplasmic membrane of e. coli. | upon infection, phage dna is transported through the bacterial cytoplasmic membrane. this crossing is accompanied by a transient increase in the permeability of the cytoplasmic membrane toward ions and small solutes. this has led several authors to propose that dna might cross the cytoplasmic membrane through channels. in the first part of the review we present data that we obtained with phage t4 and that strongly support this proposal. we then present the structural and ionic characteristics of ... | 1989 | 2470417 |
| [graves' disease in childhood]. | authors describe clinic and therapeutic results in seven hyperthyroid graves' disease patients between ages of three and a half an 15 and a half (x: 11.2) years relating their results with those of literature. most peculiar aspects of these cases were pretibial myxedema in two patients and lack of goiter in one of them. results include t4 greater than 18.5 mcg/100 ml in five, t3 greater than 645 ng/100 in three and a relationship between t3/t4 greater than or equal to 20 in five. these results h ... | 1988 | 2469369 |
| role of exonuclease iii and endonuclease iv in repair of pyrimidine dimers initiated by bacteriophage t4 pyrimidine dimer-dna glycosylase. | the role of exonuclease iii and endonuclease iv in the repair of pyrimidine dimers in bacteriophage t4-infected escherichia coli was examined. uv-irradiated t4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. t4 denv phage were equally sensitive when plated on wild-type e. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as t4 pyrimidine dimer-dna glycosylase. a uvra mut ... | 1989 | 2468648 |
| stability of group i intron rna in escherichia coli and its potential application in a novel expression vector. | intron rna excised from the primary transcript of the phage t4 td gene was found to be unusually stable in vivo. in contrast to the average half-life of about 1.5 min for a typical escherichia coli mrna at 37 degrees c, the half-life of the excised group-i td intron ranged from 12 to 19 min for the linear form and from 22 to 33 min for the circular form. a 631-nucleotide region of the intron that is not essential for splicing was replaced by the chloramphenicol acetyltransferase (cat) structural ... | 1988 | 2468580 |
| symmetric transcription of bacteriophage t4 base plate genes. | dot-blot and northern-blot experiments, using strand-specific rna probes, show that part of the bacteriophage t4 dna that codes for six of the base plate structural genes (gp 51, 27, 28, 29, 48 and 54), is transcribed in vivo from both dna strands. the r dna strand transcripts contain sequences which are translated into structural proteins. antisense l strand rna is about 100 fold less abundant than rna molecules transcribed from the r dna strand. | 1988 | 2468563 |
| genes within genes: independent expression of phage t4 intron open reading frames and the genes in which they reside. | the td, nrdb, and suny introns of bacteriophage t4 each contain a long open reading frame (orf). these orfs are preceded by functional t4 late promoters and, in the case of the nrdb intron orf, a functional middle promoter. expression of phage-encoded intron orf-lacz fusions indicates that these t4 genes are highly regulated. the lack of translation of these orfs from early pre-mrnas can be accounted for by the presence of secondary structures that are absent from the late rnas. because translat ... | 1988 | 2467840 |
| trypsin cleavage in the cooh terminus of the bacteriophage t4 gene 41 dna helicase alters the primase-helicase activities of the t4 replication complex in vitro. | the bacteriophage t4 gene 41 protein is a 5' to 3' dna helicase which unwinds dna ahead of the growing replication fork and, together with the t4 gene 61 protein, also functions as a primase to initiate dna synthesis on the lagging strand. proteolytic cleavage by trypsin approximately 20 amino acids from the cooh terminus of the 41 protein produces 41t, a 51,500-dalton fragment (possibly still associated with small cooh-terminal fragments) which still retains the ssdna-stimulated gtpase (atpase) ... | 1989 | 2466840 |
| pediatric hodgkin's disease: pulmonary, cardiac, and thyroid function following combined modality therapy. | since pediatric hodgkin's disease is a curable malignancy, it is essential to limit treatment sequelae. this study examines post-treatment pulmonary, cardiac, and thyroid function in 34 children, ages 5 to 17 (23 male and 11 female) with hodgkin's disease. all received combined modality therapy of 6 cycles of alternating abvd/mopp chemotherapy and low dose (1500-2500 cgy) involved field radiotherapy. mean follow-up period is 27.5 months with actuarial freedom from relapse of 94% and survival of ... | 1989 | 2466027 |
| binding of labeled thyroxin analog to serum proteins evaluated after radioimmunoassay of free thyroxin. | in ambulatory patients, assay of free thyroxin (ft4) in serum correlates well with thyroid status and with results obtained by equilibrium dialysis. the validity of ft4 results has been questioned mainly in euthyroid patients with altered concentrations of thyroid hormone-binding proteins, as in nonthyroidal illness, hereditary analbuminemia, familial dysalbuminemic hyperthyroxinemia (fdh), and the presence of iodothyronine-binding antibodies. i present here a study of the binding of [125i]t4-de ... | 1989 | 2465846 |
| flow cytometric method to demonstrate whether anti-hiv-1 agents inhibit virion binding to t4+ cells. | the first step in the replicative cycle of human immunodeficiency virus type 1 (hiv-1) is binding of the virions to the cellular cd4 receptor. this process may be considered as an important target for chemotherapeutic agents against acquired immune deficiency syndrome (aids). a method has now been devised whereby virion binding to the cell membrane was visualized by an indirect immunofluorescence assay using human anti-hiv-1 serum, rabbit anti-human-ig-f(ab')2-fluorescein isothiocyanate, and flo ... | 1989 | 2465402 |
| induction cytotoxic chemotherapy: the significance of apparent complete clinical regression after using the price hill schedule a protocol for squamous carcinoma of the head and neck. | a small group of 46 previously untreated patients out of about 260 in all who have received price hill schedule a cytotoxic chemotherapy, achieved apparent complete clinical regression (accr) of their disease before local treatment. forty patients were classified t3 or t4, over half having clinically positive nodes at the start of treatment. a determinate survival rate of 70 per cent disease free at three years was obtained and 74 per cent achieved quality relief of all symptoms. two patients de ... | 1988 | 2465362 |
| sequestration of virus-specific t cells in the cerebrospinal fluid of a patient with varicella zoster viral meningoencephalitis. | the frequency of virus-specific t cells in the cerebrospinal fluid of a patient with viral infection of the brain and meninges was determined by using a single-t-cell cloning technique where a representative sampling of t cells was cloned from the cerebrospinal fluid of a patient with varicella zoster viral (vzv) meningoencephalitis. that the derived t-cell clones were in fact clonal was shown by demonstrating, on southern blot analyses, unique rearrangements of the t-cell antigen-receptor beta- ... | 1989 | 2465314 |
| in vitro functional blocking of myelin basic protein-specific cytolytic human t lymphocyte clones by immunosuppressive drugs and monoclonal antibodies. | the in vitro effects of cyclosporin a, prednisolone, and anti-cd4 monoclonal antibody ww.t4 on myelin basic protein-specific human cd4+ cytolytic t lymphocyte clones were studied. functional assays of antigen-specific proliferation, induction of specific lysis, cytolysis itself, and interferon-gamma production were done. prednisolone decreased secretion of interferon-gamma by the clones and blocked specific proliferation; the latter could, however, be overcome by the addition of exogenous interl ... | 1989 | 2465309 |
| inhibitory effect of lithium on the release of thyroid hormones from thyrotropin-stimulated mouse thyroids in a perifusion system. | we studied the effect of lithium on the release of t3, t4, and camp from perifused mouse thyroids and on camp content in thyroid pieces. lithium significantly inhibited t3 and t4 release from tsh-stimulated mouse thyroids. this inhibitory effect on thyroid hormone release was dependent on the concentration of lithium. under continuous stimulation with tsh and 3-isobutyl-1-methylxanthine, both camp release and camp content were significantly decreased by lithium. in addition, we studied the effec ... | 1989 | 2465138 |
| unusually high frequencies of hiv-specific cytotoxic t lymphocytes in humans. | ctl specific for the hiv belong to the cd8 subset of t lymphocytes, and their activity is restricted by class i hla transplantation ag. in this report, hiv-specific ctl and their precursor cells were quantified by limiting dilution analysis. ctl were recovered from the lungs, lymph nodes, and blood of asymptomatic seropositive carriers and of patients with aids. hiv was found to be very immunogenic. high frequencies of both hiv-specific ctl and ctl precursor cells were detected in infected indiv ... | 1989 | 2463308 |
| amplification of suppressor inducer pathway with monoclonal antibody, anti-2h4, identifying a novel epitope of the common leukocyte antigen/t200 antigen. | the 2h4 antigen, comprised of a 200/220-kda glycoprotein of the leukocyte common antigen (lca) family, is expressed on a suppressor inducer, but not a helper inducer subset of t4 cells. earlier studies have demonstrated that the t4+2h4+ subset of cells maximally responded to the amlr and this molecule has an important role in generated suppressor signals in amlr/con a-activated t cell systems. in the present study, we examined the effect of a series of monoclonal antibodies including anti-2h4 an ... | 1989 | 2463098 |
| evidence for an involvement of t4+ cytotoxic t cells in tumor immunity. | cell-mediated immunity against cancer cells primarily involves class i major histocompatibility complex (mhc)-restricted cytotoxic t cells and natural killer (nk) cells. to investigate whether t4+ cytotoxic t cells also have a role in tumor-specific immunity, mice were immunized with a b cell lymphoma. t cell hybridomas were constructed from the immune spleen cells and analyzed for their cytotoxic ability against the immunizing lymphoma. a t4+, lyt-1+ hybridoma cell line was developed (103l2) wh ... | 1989 | 2463095 |
| identification and importance of lymphocyte subpopulations in the regional lymph nodes of breast cancer patients. | to determine whether the presence of metastatic cancer cells in lymph nodes is accompanied by changes in lymphocyte subpopulations identified in tissue sections, the authors studied metastatic and nonmetastatic lymph nodes from eight patients with breast cancer and lymph nodes of three control patients. in all metastatic lymph nodes, t cells were seen in close contact with infiltrating cancer cells; b cells tended to accumulate focally, apart from cancer cell nests. in both metastatic and nonmet ... | 1989 | 2463069 |
| characterization of the binding specificity of two anticruciform dna monoclonal antibodies. | two monoclonal antibodies (2d3 and 4b4) have been raised against a stable cruciform dna structure containing the 27-base pair palindrome of the sv40 origin of replication on one strand and an unrelated 26-base pair palindrome on the complementary strand (prgm 21 x prgm 29) and have been shown to recognize conformational determinants specific to cruciform dna structures (frappier, l., price, g.b., martin, r. g., and zannis-hadjopoulos, m. (1987) j. mol. biol. 193, 751-758). to define the region(s ... | 1989 | 2462559 |
| evaluation of thyroid gland activity by hormone assays and flow cytometry in rats. | spontaneous variations in the activity of thyroids and changes induced by methimazole, phenobarbital and 2,4--diaminoanisole sulfate were assessed in rats by repeated measurements of serum t3, t4 and tsh concentrations and cell cycle analysis in isolated nuclei of the thyroids. methimazole caused a decrease in t3 and t4, and elevation in tsh, reduction of cells in g1 and increase in the percentage of cells in s and g2 phases. with phenobarbital t4 concentrations were decreased, but a mitogenic e ... | 1988 | 2462517 |
| [ambivalent effect of thyroxine on the expression of gonadotropin genes in normal and orchidectomized rats]. | in order to examine the role of thyroid hormones on pituitary gonadotropin synthesis and release, normal, thyroidectomized and orchidectomized male rats were treated in parallel by daily injections of thyroxine (10 micrograms/10 g). pituitary levels of alpha, lh beta, fsh beta and tsh beta mrna were determined using two complementary approaches: 1) cell-free translation of pituitary mrna and quantitative immunoprecipitation of labeled subunit precursors, and 2) hybridization with [32p]-labeled c ... | 1988 | 2462206 |
| the effects of lesions of superior temporal gyrus and inferior parietal lobe on temporal and vertex components of the human aep. | we recorded auditory evoked potentials (aeps) to 1 khz tone bursts in controls and patients with unilateral lesions centered in posterior superior temporal gyrus and adjacent caudal inferior parietal lobule (stg) or in rostral inferior parietal lobule (ipl). controls generated a vertex maximal n94 (n1b) and p200 (p2) and additional p45, n78 and n127 temporal aep components (p45, n1a, n1c). similar to prior reports, in controls the n1a was most prominent over the left temporal lobe and the p45 wa ... | 1988 | 2461284 |
| rapid and automated tetrazolium-based colorimetric assay for the detection of anti-hiv compounds. | a rapid, sensitive and automated assay procedure was developed for the in vitro evaluation of anti-hiv agents. an htlv-i transformed t4-cell line, mt-4, which was previously shown by koyanagi et al. (1985) to be highly susceptible to, and permissive for, hiv infection, served as the target cell line. inhibition of the hiv-induced cytopathic effect was used as the end point. the viability of both hiv- and mock-infected cells was assessed spectrophotometrically via the in situ reduction of 3-(4,5- ... | 1988 | 2460479 |
| xenopus transcription factor iiia-dependent dna renaturation. | kinetic and titration analyses are used to elucidate the mechanism by which xenopus transcription factor iiia (tfiiia), a protein required for 5 s rna synthesis by rna polymerase iii, promotes dna renaturation. tfiiia promotes 50% renaturation of complementary strands (303 bases) in 45 s. analyses of the renaturation kinetics indicate the rate-limiting step in this tfiiia-dependent reaction is first order. tfiiia-dependent dna renaturation is a stoichiometric rather than a catalytic process. the ... | 1988 | 2460459 |
| the different effect of alpha and gamma interferons and interleukin 2 on the expression of cd2, cd3, cd4 and cd8 antigens in comparison to histocompatibility antigens of human lymphocytes. | the effects of alpha- and gamma-interferons (ifn-alpha, -gamma) and of interleukin 2 (il-2) on the expression of certain differentiation antigens were compared with those of major histocompatibility antigens on human lymphocytes. ifn-gamma and ifn-alpha in high doses significantly increased the expression of t11 (cd2) differentiation antigen, but did not affect the expression of t4 (cd4), t8 (cd8), t3 (cd3) and leu-7 antigens (hnk-1). both natural and recombinant ifn-alpha and -beta apparently i ... | 1988 | 2460402 |
| [changes in the hormone content of the blood during chronic electrical stimulation of the midbrain]. | the levels of hormones of the hypophysis (acth, tth, cth), thyroid gland (t3, t4), adrenal glands (epinephrine, norepinephrine, cortisol) and pancreas (insulin, glucagon) increased during a prolonged electrostimulation of the reticular nucleus of mesencephalon in rabbits. administration of the substance p returned the hormonal levels to normal. | 1988 | 2460380 |
| a novel procedure for selective cloning of noti linking fragments from mammalian genomes. | a novel procedure has been developed for selective cloning of noti linking fragments from mammalian genomes. since the majority of the noti sites in mammalian genomes are considered to be localized in so-called htf (hpaii tiny fragment) islands, an htf library was constructed as an initial step to enrich the noti sites. the plasmid dnas were isolated en masse from the htf library and digested with noti. linearized plasmid dnas derived from noti linking clones were efficiently separated from undi ... | 1988 | 2459661 |
| lipolysis of ldl with phospholipase a2 alters the expression of selected apob-100 epitopes and the interaction of ldl with cells. | to assess the effects of perturbing the surface of low density lipoprotein (ldl) on the conformation of apob-100, ldl (d 1.030-1.050 g/ml) isolated from normal subjects were treated with phospholipase a2 (pl-a2) for 0.5 to 15 min. the resulting p-ldl and concurrent control ldl (c-ldl) incubated without pl-a2 were isolated by gel permeation chromatography. approximately 50% of ldl-phosphatidylcholine was hydrolyzed in 2 min and approximately 85% in 5 min. lysophosphatidylcholine compounds (lpc) a ... | 1988 | 2459282 |
| comparative removal of pyrimidine dimers from human epidermal keratinocytes in vivo and in vitro. | we have compared the kinetics for repair of uv-induced cyclobutane pyrimidine dimers in the dna of keratinocytes in human skin and in cell culture. a small area of the buttocks of volunteers was exposed to uvb-irradiation and biopsies were taken at various time intervals. epidermal keratinocytes in culture from the same subjects were exposed to uvc with doses chosen to elicit comparable yields of dimers in cellular dna. the initial density of pyrimidine dimers and the kinetics of their removal w ... | 1988 | 2459263 |
| antiproteases are increased in bronchoalveolar lavage in interstitial lung disease. | the present study evaluates different cellular and soluble components in the bronchoalveolar lavage (bal) from patients with interstitial lung disease. we observed an increased t4/t8 lymphocyte ratio in bal but not in blood from 24 patients with active pulmonary sarcoidosis compared to sixteen normal individuals and to eleven patients with inactive pulmonary sarcoidosis. seven patients with hypersensitivity pneumonitis had a normal t4/t8 ratio. in the active sarcoidosis and hypersensitivity pneu ... | 1988 | 2458961 |
| functional consequences of the arabinosylcytosine structural lesion in dna. | cytosine arabinoside (arac) is a potent antileukemic agent that is misincorporated into dna in the course of its action. we have developed a chemical synthetic method that allows site-specific introduction of arac into synthetic dna oligomers. we describe here the utilization of these oligomers as primer/template substrates for in vitro dna synthesis reactions and as fragments for dna ligation. these studies were undertaken to investigate the manner in which sites of arac misincorporation consti ... | 1988 | 2458756 |
| protein synthesis and quantitative morphology in thyroid tissue from hyperthyroid patients after preoperative treatment with antithyroid or beta-adrenergic antagonist drugs. | the rate of protein synthesis was measured in thyroid tissue obtained from three groups of patients undergoing thyroid surgery: group i (n = 18), hyperthyroid patients preoperatively treated with an antithyroid drug and t4; group ii (n = 11), hyperthyroid patients preoperatively treated with a beta-adrenergic antagonist drug; and group iii (n = 9), euthyroid patients operated on for multinodular goiter or adenoma. quantitative morphology was studied in the resected thyroid tissue from the patien ... | 1988 | 2458376 |
| regional specificities of monoclonal anti-human apolipoprotein b antibodies. | the usefulness of monoclonal antibodies as probes of protein structure is directly related to knowledge of the structures and locations of the epitopes with which they interact. in this report we provide a detailed map of 13 epitopes on apob-100 defined by our anti-apob monoclonal antibodies based on current information on the amino acid sequence of apob-100. to localize antibody specificities to smaller regions along the linear sequence of the apob-100 molecule we used a) thrombin- and kallikre ... | 1988 | 2457642 |
| [determining the triiodothyronine uptake and its derivative values-t4, t3 and tbg indices-based on labeled t3 binding by a mixture of activated charcoal and cellulose]. | 1987 | 2457494 | |
| effect of preoperative iodine in patients with graves' disease controlled with antithyroid drugs and thyroxine. | thirty-four patients with graves' disease, first rendered euthyroid with antithyroid drugs (atd) then given supplementary thyroxine (t4), were randomly allocated to three treatment groups. in group i atd and t4 were stopped 10 days before partial thyroidectomy and the patients were treated with lugol's iodine alone. in group ii the patients were treated up to the time of operation with atd and t4 alone. in group iii atd and t4 were continued until the day of operation, but the patients also rece ... | 1988 | 2457351 |
| analysis of cleavage products of dna repair enzymes and other nucleases. characterization of an apurinic/apyrimidinic specific endonuclease from mouse plasmacytoma cells. | we have developed a strategy by which the nature of phosphodiester bond breaks produced by various dna-repair endonucleases and also other nucleases, can be characterized. a purified apurinic/apyrimidinic (ap) specific endonuclease from a permanently established mouse plasmacytoma cell-line (mpc-11) has been examined with respect to the exact incision site generated at the baseless site. by the aid of enzymatic treatment with calf intestinal phosphatase, the 3'-phosphatase activity of t4-polynuc ... | 1988 | 2457203 |
| the immunological profile in sickle cell anaemia: a study of patients in the arab peninsula. | recurrent and often serious infections are common in sickle cell anaemia. the predisposing causes are multiple, and immune abnormalities are frequently blamed. in this study, immune complexes, lymphocyte subpopulations, immunoglobulins and complement were determined in 40 arabs with sickle cell anaemia to ascertain some aspects of their immunological profile. immunoglobulins were found to be either normal or high, c3 and c4 values mostly clustered at the lower level of normal with a few values b ... | 1988 | 2456728 |
| detection of an epitope, not required for polymerization, that is conserved between e.coli dna polymerases i and iii and bacteriophage t4 dna polymerase. | monoclonal antibodies directed against the alpha subunit of the dna polymerase iii holoenzyme (1) of e. coli were tested for cross-reactivity with a variety of polymerases. we found that one monoclonal antibody bound to e. coli dna polymerase i as well as to dna polymerase iii. a weaker, but specific, interaction was also detected with t4 dna polymerase. we exploited the proteolysis procedure developed by setlow, brutlag and kornberg (2) to determine which domain of dna polymerase i contained th ... | 1988 | 2456526 |
| [a case of chemical hyperthyroidism induced by antiarrhythmic agent amiodarone]. | amiodarone, an antiarrhythmic agent, is known to occasionally induce alterations in thyroid function because of its iodine content and ability to inhibit t4 5'-monodeiodination. we herein describe the drug-induced chemical hyperthyroidism in a diabetic patient with ventricular premature beats. a 46-year-old man with well controlled diabetes mellitus revealed neck swelling during a 4 months' treatment with amiodarone for his frequent occurrence of ventricular premature beats. physical findings we ... | 1988 | 2456231 |
| characterization of thyroid hormone effects on na-k pump and membrane potential of cultured rat skeletal myotubes. | the purpose of this study was to characterize the effects of thyroid hormone on the na-k pump and resting membrane potential (em) of rat skeletal myotubes in culture. myotubes were obtained from fetal (19-21 day) or neonatal rats (1-2 day) by serial trypsinization and maintained in culture for up to 10 days. cells were treated with t4 or t3 on day 6 or 7, and measurements were made of em, [3h]ouabain binding, and ouabain-sensitive 86rb uptake at various times thereafter. hormone treatment increa ... | 1988 | 2456206 |
| the cd4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human t lymphocytes. | the cd4 (t4) antigen is a cell-surface glycoprotein that is expressed predominantly on the surface of helper t cells and has been implicated in the regulation of t-cell activation and in the associative recognition of class ii antigens of the major histocompatibility complex. in addition, the cd4 antigen appears to serve as a receptor for the human immunodeficiency virus (hiv). an important question has been whether the cd4 receptor is linked to an intracellular mediator that could regulate the ... | 1988 | 2455897 |
| an okazaki piece of simian virus 40 may be synthesized by ligation of shorter precursor chains. | it is generally accepted that an aphidicolin-sensitive dna polymerase elongates the eucaryotic rna primer (irna) into a mature okazaki piece reaching ca. 200 nucleotides. yet, as shown here, nascent dna chains below 40 nucleotides accumulated in simian virus 40 (sv40) dna replicating in isolated nuclei in the presence of aphidicolin. these products resembled precursors of longer okazaki pieces synthesized in the absence of aphidicolin (termed here dna primers) in size distribution, lagging-repli ... | 1988 | 2455822 |
| the effect of altered thyroid function on serum fructosamine concentrations. | the effect of altered thyroid function on serum fructosamine concentrations was investigated in 31 untreated hyperthyroid patients, 18 short-term hypothyroid patients (i.e., 20 days after withdrawal of thyroid hormone suppressive therapy for thyroid cancer), 7 untreated long-term hypothyroid patients, and 25 age-matched normal controls. no differences in serum fructosamine concentrations were observed between hyperthyroid patients and normal controls; conversely, serum fructosamine concentration ... | 1988 | 2455610 |
| degradation of structurally modified dnas by bleomycin group antibiotics. | bleomycin-mediated dna strand scission has been shown to be diminished at certain sequences in proximity to 5-methylcytidines. we have investigated the molecular basis of this observed diminution using selective bleomycin (blm) modifications at the c-terminus. of the four different bleomycin congeners investigated, only bleomycin a2 and bleomycin bapp were substantially affected by cytidine methylation. we have also examined the effect of other dna modifications on bleomycin-mediated strand scis ... | 1988 | 2455539 |
| origin of sympathetic and sensory innervation of the elbow joint in the rat: a retrograde axonal tracing study with wheat germ agglutinin conjugated horseradish peroxidase. | after injection of wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp) into the elbow joint of adult rats, labeled neurons were found in the stellate and the t2-t4 ganglia of the ipsilateral sympathetic trunk, and also in dorsal root ganglia at the c4-t4 levels. most labeled sympathetic cells, 90% or more, were located in the stellate ganglion. the sensory innervation to the joint originated mainly from the dorsal root ganglia at the levels of c7-t1. | 1988 | 2454963 |
| characterization of ion channels involved in the penetration of phage t4 dna into escherichia coli cells. | the hypothesis of a channel-mediated transport of phage dna into escherichia coli cytoplasmic membrane has been formulated for a long time. in this paper, we present experimental evidence in favor of this proposal. we have analyzed the kinetics of the k+ efflux induced by t4 phage and ghosts (phage depleted of dna) using a potassium selective electrode. we show that the k+ efflux is not catalyzed by the k+ transport systems. the km of k+ efflux is the same for phage and ghosts. the rate of k+ ef ... | 1988 | 2454920 |
| neoadjuvant chemotherapy in previously untreated patients with advanced head and neck squamous cell cancer. | between may 1981 and january 1986, 130 consecutive patients with advanced untreated head and neck squamous cell cancer (hnscc) received neoadjuvant chemotherapy (nac) with two or three courses of the cabo combination (methotrexate 40 mg/m2 intravenously (iv) on days 1 and 15; cddp 50 mg/m2 iv on day 4; bleomycin 10 mg iv on days 1, 8, and 15; and vincristine 2 mg iv on days 1, 8, and 15 every 3 weeks) prior to surgery and/or radiotherapy. of the 123 patients evaluable for response to chemotherap ... | 1988 | 2454721 |
| anti-cd4 and anti-cd2 monoclonal antibody infusions in subjects with multiple sclerosis. immunosuppressive effects and human anti-mouse responses. | the immunologic responses to anti-t4 and anti-t11 mab infusions were assessed in subjects with chronic progressive multiple sclerosis as part of phase i clinical studies. eight subjects received five daily infusions (0.2 mg/kg/day) of either anti-t11 (cd2) or anti-t4 (cd4) murine mab. it was found that in vivo anti-t cell mab infusions specifically suppress in vitro measurements of the human immune response; anti-t11 mab blocked t cell activation via the cd2 srbc-binding protein, and anti-t4 mab ... | 1988 | 2454256 |
| immunohistochemistry of lymphocytes and langerhans' cells in long-lasting allergic patch tests. | a long-lasting allergic patch test is a "normal" allergic patch test that remains positive for weeks or months. an immunohistochemical study of immunocompetent cells in the skin in this rare type of patch tests was performed. most inflammatory cells were t11 positive t-lymphocytes. the majority of these cells were of the helper/inducer phenotype (t4+), but a relative increase of t8+ cells as compared to the initial (1-2d) stages of allergic patch tests was observed. t6+ langerhans' cells (lcs) w ... | 1988 | 2453989 |
| the organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the z line extends close to the m line. | mabs specific for titin or nebulin were characterized by immunoblotting and fluorescence microscopy. immunoelectron microscopy on relaxed chicken breast muscle revealed unique transverse striping patterns. each of the 10 distinct titin antibodies provided a pair of delicate decoration lines per sarcomere. the position of these pairs was centrally symmetric to the m line and was antibody dependent. the results provided a linear epitope map, which starts at the z line (antibody t20), covers five d ... | 1988 | 2453516 |
| hypothyroidism increases substance p concentrations in the heterotopic anterior pituitary. | the regulatory effects of thyroid hormone on adenohypophysial substance p (sp) were studied in heterotopically implanted anterior pituitaries. three or four anterior pituitaries from 21-day-old rat pups were implanted under the renal capsule in 175- to 200-g adult rats. the donor and recipient animals were sex matched. one week after implantation, animals were thyroidectomized or sham operated. a separate group of animals received daily t4 treatment (1.5 g/100 g, ip). after 2 weeks, the native a ... | 1988 | 2453352 |
| dependence of m1 rna substrate specificity on magnesium ion concentration. | we have constructed a plasmid expressing e. coli m1 rna, the catalytic rna subunit of ribonuclease p, under the control of a phage t7 promoter. the active m1 rna species synthesized in vitro by t7 rna polymerase from this vector was reacted with the trna(gln) - trna(leu) precursor rna (band k) encoded by phage t4. only the trna(leu) moiety of this dimeric precursor rna contains the 3' terminal c-c-a sequence common to all trnas. we observed that protein-free m1 rna was capable of processing the ... | 1988 | 2453026 |
| activation of t lymphocytes by immobilized monoclonal antibodies to cd3. regulatory influences of monoclonal antibodies to additional t cell surface determinants. | the effect of soluble or immobilized mab directed at various additional surface proteins on the proliferation of highly purified t4 cells induced by two immobilized mab to cd3, okt3 and 64.1, was examined. high density 64.1 stimulated nearly all t4 cells to enter and progress through the cell cycle. maximal t4 cell proliferation required stimulation with immobilized 64.1 throughout the length of the incubation and was not effected by any of the additional soluble or immobilized mab employed. in ... | 1988 | 2452835 |
| hiv-1 expression by t8 lymphocytes after transfection. | acquired immune deficiency syndrome (aids) is an immunosuppressive disease associated with the depletion of t4 lymphocytes. recently, an hiv-1 genome was molecularly cloned and shown to be fully infectious in vitro by transfection experiments. in this study, we show that hiv-1 can be transfected into t4 and t8 lymphocyte subpopulations and viral proteins and infectious virus particles are observed in both short- and long-term cultures. in addition, transfected t8 cells can be maintained in cultu ... | 1988 | 2452649 |
| [immunological profile of whipple's disease evolving over a period of 17 years]. | this report describes an immunological study made on a 58 years old patient with a whipple disease diagnosed in 1969 and treated with different antibiotics. all attempts to stop the antibiotherapy resulted in reappearance of clinical symptoms. further, this patient suffered anguillulosis infection in 1954 and this persists despite thiabendazole therapy, as shown by periodical creeping lunear dermatitis (larva currens). laboratory investigations displayed low igm levels and lack of cutaneous reac ... | 1988 | 2452591 |
| the lit gene product which blocks bacteriophage t4 late gene expression is a membrane protein encoded by a cryptic dna element, e14. | escherichia coli lit(con) mutations cause a severe inhibition of gene expression late in infection by bacteriophage t4 owing to the overproduction of one, and possibly two, proteins (c. kao, e. gumbs, and l. snyder, j. bacteriol. 169:1232-1238, 1987). one or both of these proteins interact, either directly or indirectly, with a short sequence about one-quarter of the way into the major capsid protein gene of t4, and the inhibition occurs when this late gene of the virus is expressed. in this rep ... | 1988 | 2452152 |
| interferons (ifns) and tumor necrosis factors (tnfs) in t cell-mediated immune responses against alloantigens. i. influence on the activation of resting and antigen-primed t cells. | the aim of this study was to investigate the influence that endogenous ifns released in response to antigenic or viral stimuli has on recognition of alloantigens in mlc. results indicated that both the magnitude and the kinetics of response can be modified by ifns. neutralizing antibodies with specificity for ifn-gamma inhibit early and enhance late proliferative responses in mlc. addition of physiological concentrations of ifn-gamma enhanced both early and peak proliferation, whereas ifn-alpha ... | 1988 | 2452129 |