Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| stimulation of calcium-atpase activity by 3,5,3'-tri-iodothyronine in rat thymocyte plasma membranes. a possible role in the modulation of cellular calcium concentration. | we have previously demonstrated that 3,5,3'-tri-iodo-l-thyronine (t3) produces a very rapid and transient increase in calcium uptake and cytoplasmic free calcium concentration in the rat thymocyte, and have postulated that ca2+-atpase may contribute to the overall effect of t3 on cellular calcium metabolism. in the present study, we show that in the rat thymocyte, t3 increased plasma membrane ca2+-atpase activity. this effect of t3 was very rapid, seen at 30 s after the addition of the hormone, ... | 1989 | 2529849 |
| delta-elimination in the repair of ap (apurinic/apyrimidinic) sites in dna. | [5'-32p]pdt8d(-)dt7, containing an ap (apurinic/apyrimidinic) site in the ninth position, and [d(-)-1',2'-3h, 5'-32p]dna, containing ap sites labelled with 3h in the 1' and 2' positions of the base-free deoxyribose [d(-)] and with 32p 5' to this deoxyribose, were used to investigate the yields of the beta-elimination and delta-elimination reactions catalysed by spermine, and also the yield of hydrolysis, by the 3'-phosphatase activity of t4 polynucleotide kinase, of the 3'-phosphate resulting fr ... | 1989 | 2529848 |
| [effect of teicoplanin and vancomycin on cerebrospinal fluid proteins of non-infected rabbits after suboccipital injection]. | the ic inoculation of antibiotics into the csf for therapeutic use could produce biological effects we should consider when analysing samples. to corroborate this assumption, we observed the effect of ici t and v on the pl of the csf of non infected rabbits. t and v were ici as dosage of 1 mg/kg diluted in 0.2 ml of isotonic saline solution (iss). iss was also ici alone. csf samples were obtained before inoculation from 41 animals (t0) setting the normal pl. other samples were obtained 2 (t2) an ... | 1989 | 2528711 |
| cross-reactivity between human and canine helper and suppressor t cell antigens using monoclonal antibodies rpa-t4 and huly-m8. | the murine monoclonal antibodies rpa-t4 and huly-m8, specific for a framework determinant of human helper/inducer and suppressor/cytotoxic t cell antigens, cross-reacted with canine cell membrane molecules recognizing a biomolecular complex (50,000 to 55,000 daltons) similar to that described in humans. we investigated the distribution of these helper and suppressor t cell-like antigens on canine peripheral blood lymphocytes. with complement-mediated lymphocytotoxicity, 34% and 35% of the canine ... | 1989 | 2528406 |
| [early diagnosis of cancer of the prostate. hypothetic role of transrectal echography]. | in order to clarify the value of transrectal ultrasonic examination of the prostate in the diagnosis of prostatic cancer at an early stage, 666 patients were screened in one year; 602 of them (to) had a normal, firm or hypertrophic but non-nodular prostate at digital rectal palpation, 41 had a t1-t2 prostate and 23 a t3-t4 prostate. among the 602 to prostates, 162 showed a hypoechoic peripheral area which was biopsied, revealing an infiltrating carcinoma in 11 cases. the ability of transrectal u ... | 1989 | 2528133 |
| [expressions of 2h4 and 4b4 antigens on atl cells]. | we studied the expression of 2h4 and 4b4 on the surfaces of leukemia cells from 17 patients with adult t-cell leukemia (atl) as well as of cells belonging to 2 t-cell lines derived from atl patients. the effects of the supernatants obtained from culture fluids of the atl cells and the t-cell lines on igg production of a human b-cell line, cess cells, were also examined. on the surfaces of the atl cells from 15 out of 17 cases and of the cells of 2 t-cell lines 4b4 obviously existed at higher per ... | 1989 | 2528006 |
| [thyroid function in chronic liver diseases in an endemic region]. | iodine absorptive thyroid function, the levels of t3, t4 and tsh, and thyroid scanning were determined and performed in 132 patients with chronic liver diseases. the results of the investigation were compared with those of the control group. all patients lived in a goiter-endemic area with higher prevalence of liver diseases. functional thyroid disorders were revealed in all the patients: hypofunction in 85.6%, hyperfunction in 14.4%. structural and anatomic thyroid changes were detected in 91.6 ... | 1989 | 2527328 |
| low circulating t-helper cells in relapsing multiple myeloma. | peripheral blood t-lymphocyte subsets were studied serially over a 6 year period in 50 patients with monoclonal gammopathy of unknown significance and multiple myeloma. the results were correlated with clinical status as measured by responsiveness to treatment (response vs. relapse). to allow assessment of the effects of treatment per se upon t-lymphocyte subset levels, patients were studied prior to as well as on and off therapy. after adjustment for treatment effects, the significant correlati ... | 1989 | 2527296 |
| the regulation by thyroid hormones and androgen of epidermal growth factor synthesis in the submandibular gland and its plasma concentrations in mice. | the effects of androgen and thyroid hormones on epidermal growth factor (egf) synthesis in the submandibular gland and on plasma egf concentrations in mice were examined. testosterone propionate was administered alone or in combination with l-thyroxine (t4) to female mice for 2 weeks. the submandibular egf concentrations were increased by the administration of testosterone propionate in a dose-dependent fashion; the maximal increase, 20-fold, being produced by a dose of 2 mg every other day. the ... | 1989 | 2526843 |
| purification of limited tryptic fragments of ca2+,mg2+-adenosine triphosphatase of the sarcoplasmic reticulum and identification of conformation-sensitive cleavage sites. | sarcoplasmic reticulum membranes were treated with trypsin, and samples enriched with a1a, a1b, and c fragments (saito, k. et al. (1984) j. biochem. 95, 1297-1304), respectively, were prepared. a1b and c fragments were purified to apparent homogeneity, and an approximately equimolar mixture of a1(met1-arg198), a1a, and a1b fragments free from other contaminants was also obtained through gel permeation and hydroxylapatite chromatography in the presence of sodium dodecyl sulfate. n- and c-terminal ... | 1989 | 2526808 |
| a component of the side tail fiber of escherichia coli bacteriophage lambda can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage t4. | the distal part of the long tail fiber of escherichia coli bacteriophage t4 consists of a dimer of protein 37. dimerization requires the catalytic action of protein 38, which is encoded by t4 and is not present in the virion. it had previously been shown that gene tfa of the otherwise entirely unrelated phage lambda can functionally replace gene 38. open reading frame (orf) 314, which encodes a protein that exhibits homology to a cooh-terminal area of protein 37, is located immediately upstream ... | 1989 | 2526805 |
| nongenomic interactions of retinoic acid and thyroid hormone at the plasma membrane. | both thyroxine (t4) and 3,3'5-l-tri-iodothyronine (t3) can activate the calcium-magnesium-dependent adenosine triphosphatase (atp-ase) in plasma membranes of enucleate erythrocytes. this activation of ca2+-atpase is blocked by retinoic acid and perhaps by competing t4-binding sites on the membranes. | 1989 | 2526309 |
| structural and enzymatic studies of the t4 dna replication system. ii. atpase properties of the polymerase accessory protein complex. | in this paper we report a detailed enzymatic characterization of the interaction of the polymerase accessory protein complex of the t4 dna replication system with the various nucleic acid cofactors that activate the atpase of the complex. we show that the atpase activity of the t4 coded gene 44/62 protein complex is stimulated synergistically by binding of dna and t4 gene 45 protein and that the level of atpase activation appears to be directly correlated with the binding of nucleic acid cofacto ... | 1989 | 2526128 |
| endocrine and immune effects of melatonin therapy in metastatic cancer patients. | melatonin, the most important indole hormone produced by the pineal gland, appears to inhibit tumor growth; moreover, altered melatonin secretion has been reported in cancer patients. despite these data, the possible use of melatonin in human neoplasms remains to be established. the aim of this clinical trial was to evaluate the therapeutic, immunological and endocrine effects of melatonin in patients with metastatic solid tumor, who did not respond to standard therapies. the study was carried o ... | 1989 | 2525469 |
| the acceleration of linear dna during pulsed-field gel electrophoresis. | the velocity and orientation of t4 and lambda dna have been measured for the first 20 s during pulsed-field gel electrophoresis in order to clarify the dna motions that occur. for a square pulse with field strength e = 10 v/cm, the velocity of lambda dna increases gradually to 10.5 microns/s in 1.0 s, declines to 8.6 microns/s, and then rises to a plateau value of 9.3 microns/s after 4 s. t4 dna behaves similarly, but more slowly. parallel measurements of fluorescence-detected linear dichroism s ... | 1989 | 2525053 |
| t cells and t-cell subsets in periodontal diseases. | acetone-fixed cryostat gingival tissue sections from marginal gingivitis (mg), juvenile periodontitis (jp), adult periodontitis (ap) patients and clinically healthy subjects (h) were immunohistochemically stained with monoclonal antibodies to aid in identification and quantification of t cells and t-cell subsets in the inflammatory infiltrates. t cells were present in all specimens studied. the number of t cells in the connective tissue (ct) zone of ap was much greater than in any other groups. ... | 1989 | 2524577 |
| role of il-2 in the generation of cd4+ suppressors of human b cell responsiveness. | the capacity of human t4 cells stimulated by immobilized monoclonal antibodies to the cd3 molecular complex (64.1 and okt3) to induce and regulate b cell responsiveness was examined. t4 cells stimulated by low concentrations of immobilized 64.1 (3.0 ng/well) and all concentrations of immobilized okt3 supported b cell proliferation and differentiation. high concentrations of immobilized 64.1 (200 ng/well) failed to stimulate help but rather induced suppression by t4 cells. suppression was prevent ... | 1989 | 2523423 |
| echocardiographic studies on the relationship between atrial fibrillation and atrial enlargement in patients with hyperthyroidism of graves' disease. | in order to study the relationship between the left atrial size and atrial fibrillation (af) in patients with thyrotoxicosis, 92 patients with graves' disease were examined. out of 92 patients (23 men and 69 women, age 7-71 years), 21% had af: 31% of the patients older than 40 years had af whereas none of the patients younger than 40 years had af. echocardiography revealed that the left atrial enlargement (more than 40 mm) exist in 7% of patients younger than 40 years, in 2% of those older than ... | 1989 | 2523238 |
| uvsy protein of bacteriophage t4 is an accessory protein for in vitro catalysis of strand exchange. | the uvsx and uvsy genes are essential to genetic recombination, recombination-dependent dna synthesis and to the repair of dna damage in bacteriophage t4. purified uvsx protein has been shown to catalyze strand exchange and d-loop formation in vitro, but the role of uvsy protein has been unclear. we report that uvsy protein enhances strand exchange by uvsx protein by interacting specifically with uvsx protein: gene 32 protein (gp32) is not necessary for this effect and uvsy protein has no simila ... | 1989 | 2522995 |
| observations on t-cell sub-populations in type ii reactions. | 15 cases each of type ii reaction, ll and tt leprosy and 50 endemic controls were studied for phenotypic markers t2, t4 and t8 by 2 step immunoperoxidase technique. there was statistically significant increase in t4 (helper) cells in type ii reaction. there was also a decrease in t8 cells but this was not statistically significant. | 1989 | 2522975 |
| natural killer cell immunodeficiency in patients with chronic myelogenous leukemia. iii. defective interleukin-2 production by t-helper and natural killer cells. | even though they possess normal to increased numbers of circulating natural killer (nk) cells, patients with chronic myelogenous leukemia (cml) have a functional nk-cell deficiency which is restorable in vitro in the presence of recombinant il-2. we therefore measured the level of il-2 production by both t-helper and nk cells from cml patients as compared to normal controls using pha-stimulated peripheral blood mononuclear cells (pbms) as well as facs-sorted cd4+ (okt4+) lymphoid cells and facs- ... | 1989 | 2522912 |
| multivariate analysis of prognostic factors in patients with advanced prostatic cancer: results from 2 european organization for research on treatment of cancer trials. | the european organization for research on treatment of cancer genitourinary group performed a multivariate statistical analysis of prognostic factors based on 436 patients entered between 1976 and 1981 in 2 randomized prospective trials that compared 4 different hormonal treatment regimens. only previously untreated patients with advanced (stage t3/t4/m0 or m1) prostatic cancer were eligible. after identification of prognostic factors by means of univariate analyses a multivariate analysis using ... | 1989 | 2522561 |
| aids in arkansas. zidovudine: an overview and rationale for use. | azt is now the standard of care for all persons with aids, arc or t4 helper cell levels less than 200/mm3. some authorities are cautiously recommending it with full informed consent for patients who have fewer than 400 mm3 t4 cells or who have sustained a rapid fall in helper cells coupled with new symptoms. the optimum dosage remains to be determined. although the package insert recommends 200mg p.o. q. 4 hours, b.i.d. and q6 hour dosage regimes have been reported as being as effective with les ... | 1989 | 2522434 |
| decrease in phenotypically defined t helper inducer cells (t4+4b4+) and increase in t suppressor effector cells (t8+2h4+) in stable renal allograft recipients. | two monoclonal antibodies, anti-2h4 and anti-4b4, reciprocally divide the t4+ (cd4+) and t8+ (cd8+) lymphocytes into t4+2h4+, t4+4b4+, t8+2h4+ and t8+4b4+ subsets. the t4+2h4+, t4+4b4+ and t8+2h4+ subsets possess suppressor-inducer, helper-inducer, and suppressor-effector function, respectively, as previously defined in a system of b cell immunoglobulin production. using monoclonal antibodies, including anti-2h4 and anti-4b4, and flow cytometry, we monitored lymphocyte subpopulations in 66 renal ... | 1989 | 2522253 |
| clinical pharmacology of mivacurium chloride (bw b1090u) in children during nitrous oxide-halothane and nitrous oxide-narcotic anesthesia. | we determined the dose-response relationships of mivacurium (bw b1090u) in children (2-10 years) during nitrous oxide-halothane anesthesia (0.8% end-tidal) and during nitrous oxide-narcotic anesthesia. neuromuscular blockade was monitored by recording the electromyographic activity of the adductor pollicis muscle resulting from supramaximal stimulation at the ulnar nerve at 2 hz for 2 seconds at 10-second intervals. to estimate dose-response relationships, for each anesthetic background four sub ... | 1989 | 2521547 |
| retinoic acid is a modulator of thyroid hormone activation of ca2+-atpase in the human erythrocyte membrane. | the thyroid hormones thyroxine (t4) and 3,3',5-l-triiodothyronine (t3) stimulate plasma membrane ca2+-atpase (ec 3.6.1.3) activity in human erythrocytes by a mechanism independent of the cell nucleus. the current studies were conducted to determine the effect of retinoic acid on the extranuclear activation by t4 and t3 of ca2+-atpase in the human red cell. the retinoid inhibited basal and t4-stimulatable activity of that enzyme in a dose-dependent manner. at the highest tested concentration (10( ... | 1989 | 2521337 |
| [the effects of moxibustion at "zusanli" point on serum endocrine hormones of rats]. | the effects of moxibustion at "zusanli" acupoint on serum endocrine hormones of male wistar rats were studied by measured serum values of tsh, t3, t4, fsh, lh, testosterone and insulin with radioimmunoassay, 40 male rats were randomly divided into two groups, experiment group and control group, 20 rats each group. in the experiment group, the animals were given a moxibustion at "zusanli" acupoints of both sides of rats 8:00-9:00 am, 10 min/side per day for 7 days. the control group was given not ... | 1989 | 2521175 |
| direct and indirect techniques for free thyroxin compared in patients with nonthyroidal illness. ii. effect of prealbumin, albumin, and thyroxin-binding globulin. | we studied the correlation of thyroxin (t4)-binding proteins with the apparent free t4 (ft4) in 101 patients with nonthyroidal illness (nti). most patients (95%) were seriously ill at the time of blood collection. concentrations of t4-binding prealbumin (transthyretin), albumin, and t4-binding globulin (tbg) often were low in the sera of these patients. albumin was the most frequently subnormal, tbg the least. ft4 in serum was determined by five methods represented in 16 different assays. with f ... | 1989 | 2521137 |
| neuropsychological function in physically asymptomatic, hiv-seropositive men. | twenty asymptomatic, hiv-seropositive homosexual men and a control group of 20 seronegative homosexual men were evaluated for evidence of neuropsychological impairment. two-tailed paired t-tests of group differences revealed that the seropositive patients had significantly lower scores on two of 20 neuropsychological measures. ten seropositive patients had scores two standard deviations below the sample, compared with three seronegative patients, a significantly different distribution (p = .04). ... | 1989 | 2521073 |
| thyroid function during isoflurane anesthesia and valvular heart surgery. | proper thyroid function is essential for maintaining cardiovascular integrity during normal and stressful situations. in this study, the effects of isoflurane-o2 anesthesia and surgical stress on serum tsh, t4, free t4, t3, rt3, and cortisol were investigated in nine patients before, during, and after valve surgery. compared with preoperative control values, serum tsh decreased in the postoperative period. both t4 and free t4 had similar decreases after cardiopulmonary bypass (cpb) and remained ... | 1989 | 2520932 |
| ultrastructural and hormonal metabolic studies of rat liver maintained in vitro by perfusion at 30 degrees c and 37 degrees c: a time course study by tem, sem and ria. | isolated rat liver perfusion system has been extensively used for metabolic and functional studies. results derived from the application of this system may reflect true biochemical changes but they may also be associated with some structural changes. this study was undertaken to correlate the cytological changes and functional integrity of isolated rat liver perfused in vitro at normal physiological temperature (37 degrees c) and 30 degrees c, using a non-recirculating system. the livers were pe ... | 1989 | 2520475 |
| down regulation of hypertrophied follicular cell volume in thyroid hyperplastic gland. | in the present study, changes in thyroid follicular cell volume and its regulation have been investigated during the early involution of a hyperplastic goitre. male wistar rats were administered an iodine deficient diet for 6 months with propylthiouracil (ptu, 0.15%) during the last two months. at the end of iodine deficiency (day 0), some rats were killed and the others received a normal iodine diet. these rats were killed after different periods of iodine refeeding. thyroid follicular cell vol ... | 1989 | 2520456 |
| acquired immunodeficiency syndrome: molecular biology and its therapeutic intervention (review). | aids is one of the most perplexing diseases to confront modern medicine today. aids will rank just behind accidents, heart disease and cancer as a major cause of potential life lost in the usa by 1991. over half million aids cases are predicted by 1993 in the united states alone. there has been a great improvement in the understanding and treatment of opportunistic infections in aids. the most important concept is prophylactic treatment of the most common infectious complications as the immune s ... | 1989 | 2519841 |
| identification of 3,n4-propanodeoxycytidine 5'-monophosphate formed by the reaction of acrolein with deoxycytidine 5'-monophosphate. | acrolein reacts with deoxycytidine 5'-monophosphate at physiological ph to form one major adduct. a second minor adduct can be detected when a 3-fold excess of acrolein is present in the reaction mixture. the products were separated by ion-pair hplc on two reverse-phase columns connected in series using triethylammonium formate as ion-pair reagent. the major adduct was characterized as 3-(5'-monophospho-2'-deoxyribosyl)-7,8, 9-trihydro-7-hydroxy-pyrimido [3,4-c]pyrimidin-2-one (3,n4-propanodeoxc ... | 1989 | 2519782 |
| [pseudocyesis: clinical and hormonal evaluation]. | hormonal and clinical evaluation was performed in eleven females with diagnosis of pseudocyesis. plasma levels of pituitary gonadotropins (fsh-lh), progesterone, estradiol and thyroid hormones (t3, t4, tsh) under basal conditions were measured through radioimmunoassay. diagnosis of pseudocyesis was made according to the following criteria: a) secondary amenorrhea (greater than 12 weeks); b) two or more symptoms of gestation; c) conviction of being pregnant; d) negative hcg or pelvic ultrasound s ... | 1989 | 2518625 |
| a preliminary report on screening for congenital hypothyroidism in newborns with time-resolved fluorescence immunoassay (tr-fia) of tsh. | from november 1986 to september 1987, 27,253 newborns were screened for congenital hypothyroidism (ch) with time-resolved fluoroimmunoassay (tr-fia) for tsh concentrations in specimens of dried blood spots on filter paper. specimens were obtained in 12 hospitals by heel pricking of the babies 48-72 hours after birth. the specimens with tsh concentration higher than 20 mu u/ml were retested, and the babies were called back for confirmation of ch by tsh, t3, t4, tg serum radioimmunoassay etc. and ... | 1989 | 2517727 |
| a decrease in the equilibrium constant for the binding of thyroxine to tbg in pregnant women. | the interaction between thyroxine (t4) and thyroxine-binding globulin (tbg) was examined in the serum of pregnant subjects and compared with that in normal serum. serum t4, tbg capacity, and serum albumin concentrations were measured in serum from six normal and five pregnant subjects. the percent of free t4 (% free t4) was also measured by equilibrium dialysis against veronal buffer in which t4-binding by tbpa was completely abolished. calculated values for the net equilibrium constant for the ... | 1989 | 2517349 |
| [anorexia nervosa: endocrine function during the phases of body weight loss and recovery]. | in the present study, we investigated the endocrine status of patients with anorexia nervosa during weight loss (wl), as well as, after weight recovery (wr). a comparison between our findings and those obtained from other populations previously described was made. we studied 12 female patients during wl; 7 of them were reevaluated after wr. stimulation tests with lrh, trh, acth and insulin-induced hypoglycemia were performed in all cases. during the wl phase, basal serum levels of lh and estradi ... | 1989 | 2517152 |
| [thyroid function in normal aged subjects or with auricular fibrillation]. | free t4, free t3 and tsh before and 30 min after trh administration have been evaluated in 35 elderly patients with atrial fibrillation (af), in 35 elderly normal subjects (an) and compared with results obtained in 36 young men (yn). no significant differences were evidenced between af and an, especially no mild hyperthyroidism was discovered in af. in comparison with yn, there was no alteration of free t4 with age but free t3 is dramatically decreased; concerning tsh, there is a significant dec ... | 1989 | 2517142 |
| a general method to optimize the amount of enzyme in restriction and dna modification reactions using the beta galactosidase blue-white plaques assay. | we propose a simple and economical method for assaying the activity of restriction and other modifying enzymes. the method involves assaying the use of the blue and white colored phenotypes of bacterial colonies obtained by digesting the polylinker sequence of m13 bacteriophage vectors followed by transformation in appropriate strains on x-gal/iptg plates. in conjunction with restriction enzymes and dna ligases, the method can evaluate polymerase activity and can be applied to test 3'...5' exonu ... | 1989 | 2517016 |
| [effects of rilmenidine on arterial parameters in essential arterial hypertension]. | rilmenidine (ril) is a new antihypertensive agent which lowers sympathetic tone, so reducing systemic peripheral resistance. the effects of a single dose of ril (1 mg) on arterial function were the object of a preliminary randomised double-blind versus placebo trial in 14 patients with hypertension after a one month washout period on placebo. the following parameters were recorded before (t0) and 4 hours after administration (t4): blood pressure, heart rate (automatic recordings), diameter of th ... | 1989 | 2517009 |
| [primary plasmacytoma of the thyroid--report of an autopsy case and review of the literature in japan]. | primary extramedullary plasmacytoma (pemp) of the thyroid is rare. only nine patients with pemp of the thyroid have been reported in japan to date. we describe an additional autopsy case with pemp of the thyroid and review of the literature in japan. a 67-year-old women complaining of swelling of the anterior neck exhibited thin scalp hair, edematous face and a giant, hard, nodular goiter. laboratory examination showed elevated levels of esr, collagenous reaction, gammaglobulin, and a m-bow of t ... | 1989 | 2516813 |
| maturation of thyroid function in normal human foetuses. | the functional maturation of an independent foetal thyroid activity was investigated in the present study. serum concentrations of total t4, free t4, tsh and tbg were measured in 23 foetuses between 18 and 31 weeks' gestational age. foetal samples were collected by transabdominal needling from the placental cord insertion. tt4, ft4, tbg and tsh levels significantly increased with gestational age. ft4 levels were comparable with the adult range by 28 weeks' gestation; tbg levels reached adult val ... | 1989 | 2516787 |
| the binding of 3h-(3-mehis2) thyrotropin releasing hormone to brain and pituitary membranes of morphine tolerant-dependent and abstinent rats. | the effect of chronic administration of morphine and subsequent withdrawal on brain and pituitary receptors for thyrotropin releasing hormone (trh) was investigated in sprague-dawley rats. the rats were implanted subcutaneously with four morphine pellets (each containing 75 mg of morphine free base) during a 3-day period. placebo pellets, which contained all the excipients of morphine pellets except the morphine, were implanted in rats which served as controls. both tolerance and physical depend ... | 1989 | 2516632 |
| neonatal screening for congenital hypothyroidism in pakistan. | congenital hypothyroidism is a preventable cause of mental retardation. since clinical signs of congenital hypothyroidism do not generally become obvious before three months of age, screening programmes have been introduced in north america and europe, which consist of t4 or tsh screening on newborn infants on the third day of life. the screening for congenital hypothyroidism was initiated in pakistan by the aga khan university hospital (akuh) in march 1987. by april 1988, 5000 neonates were scr ... | 1989 | 2516533 |
| syndrome of generalized (peripheral tissue and pituitary) resistance to thyroid hormone. | generalized resistance to thyroid hormone (grth), or refetoff syndrome, is a disease in which peripheral tissues show resistance to thyroid hormone. three patients with this disease were investigated. cases 1 and 2 involved identical 7-year-old female twins and case 3, a 5-year-old girl. all three patients had goiters, and cases 1 and 2 had sensorineural deafness. in all three, the blood levels of t4, free t4, and t3 were high, while the blood levels of tsh were normal or slightly elevated. the ... | 1989 | 2516399 |
| treatment of endemic goitre due to iodine deficiency with iodine, levothyroxine or both: results of a multicentre trial. | preliminary clinical studies and recent in vitro investigations suggest that iodine administration may be an effective alternative in the treatment of the diffuse euthyroid goitre of iodine deficiency. therefore a 12-month multicentre study was initiated in which 166 patients were randomly assigned to take either 150 micrograms levothyroxine day-1 (group a, n = 61), 400 micrograms iodine day-1 (group b, n = 50), or a combination of 75 micrograms levothyroxine and 200 micrograms iodine day-1 (gro ... | 1989 | 2515973 |
| plasma thyroxine-binding proteins and thyroid hormone levels in primate species; is callithricidae thyroid hormone resistant? | thyroxine(t4)-binding to serum proteins in primates; catarrhini, prosimiae, and platyrrhini were studied by polyacrylamide gel electrophoresis t4 binding analysis. from the electrophoretic analysis, it was shown that thyroxine-binding proteins similar to human thyroxine-binding globulin (tbg) and thyroxine-binding prealbumin (tbpa) were present in catarrhini and prosimiae species, but not in platyrrhini (callithricidae and cebidae). t4-binding analysis also revealed that catarrhini and prosimiae ... | 1989 | 2515958 |
| effect of lithium carbonate administration singly or in combination with some psychotropic drugs on the radioiodide uptake by mouse thyroid. | the present study dealt with two objects; the first object was to examine whether or not lithium uptake in thyroid is modified by the thyroid state and how the intrathyroidal li affects iodide uptake by the thyroid. male and female mice were given lithium carbonate (li) with propylthiouracil (ptu) or thyroxine (t4). li was measured by a flameless atomic absorption spectrometry. the total li content in thyroid was unaffected with ptu or t4, however, li concentration per unit mass was reduced by p ... | 1989 | 2515269 |
| pituitary-thyroid axis sensitivity and neonatal changes in plasma iodothyronine, thyreostimulin and cortisol levels in the preterm lamb: comparison of two experimental models. | the influence of a 7 days prematurity, induced by oestrogen or dexamethasone injection to the mothers, on neonatal changes in plasma t4, t3, reverse t3 (rt3), tsh and cortisol levels was studied in 6 full term, 6 oestrogen preterm and 6 dexamethasone preterm lambs. in addition, the pituitary-thyroid axis sensitivity was assessed by the magnitude of the response to trh administration. at birth, plasma cortisol and t3 levels, as the value of the t3/t4 ratio, were significantly lower in the two gro ... | 1989 | 2515221 |
| a decreased capacity of hepatic growth hormone (gh) receptors and failure of thyrotrophin-releasing hormone to stimulate the peripheral conversion of thyroxine into triiodothyronine in sex-linked dwarf broiler hens. | the effect of two different doses of thyrotrophic releasing hormone (trh) upon the plasma levels of growth (gh) and thyroid hormones in both sex-linked dwarf (dw) and normal (dw) broiler hens was determined. in normal hens, 1.5 and 24 microg trh/kg increased the gh plasma concentrations after 15 min. plasma concentrations of t3 increased significantly 1 h after trh injection, whereas t4 concentration decreased after 2 following injection of 24 microg/kg trh. in dwarf hens both doses of trh incre ... | 1989 | 2514704 |
| thyroid hormone and growth: relationships with growth hormone effects and regulation. | for some years, research in the field of growth endocrinology has been mainly focused on growth hormone (gh). however, it appears that gh does not always control growth rate. for instance, it does not clearly influence intra-uterine growth: moreover, although the results of grf or gh administration appear convincing in rats, pigs or heifers, this is not the case in chickens and lambs. in addition, gh does not always clearly stimulate somatomedin production, particularly diring food restriction a ... | 1989 | 2514702 |
| an expansile secondary hypophyseal mastocytoma in a dog. | a 5-year-old mixed breed dog was presented with a history of depression and anorexia. physical examination revealed a pharyngeal tumour and a neurological examination indicated the presence of a possible space-occupying lesion in the brain. investigative procedures included a bloodsmear, impression smears and cytology of the pharyngeal tumour, haematology, chemical pathology, faecal analysis, urinalysis, electrocardiography, cerebrospinal fluid analysis, hormone assays and a computerised axial t ... | 1989 | 2514266 |
| the endocrine and metabolic response to feeding in the developing lamb. | oxygen consumption (vo2), carbon dioxide production (vco2), heart rate and the arterial plasma concentrations of glucose, non-esterified fatty acids, thyroxine (t4), 3,5,3'-tri-iodothyronine (t3), tsh, insulin, cortisol and gh were measured before and after feeding a formula milk diet to lambs aged 9, 21, 33 and 46 days. in all age groups, vo2, vco2 and heart rate increased significantly following feeding and this effect was greatest at 9 days of age. both vo2 and vco2 before and after feeding d ... | 1989 | 2514244 |
| recombinant human interferon-beta suppresses the replication of hiv and acts synergistically with azt. | the in vitro effects of recombinant human interferon-beta ser (rifn-beta ser) and 3'-azido-3'-deoxythymidine (azt) alone and in combination on human immunodeficiency virus (hiv) replication were examined. rifn-beta ser inhibited hiv progeny virus synthesis in cell cultures chronically infected with hiv. when used in combination, suboptimally effective concentrations of rifn-beta ser and azt synergistically inhibited hiv-mediated syncytium formation in hela t4 cell cultures. whereas azt alone red ... | 1989 | 2514233 |
| disturbed endochondral ossification in the axial skeleton of young broiler fowls. | physeal lesions have been studied in thoracic vertebrae (t3, t4 and t5) of 51 broiler fowls between 28 and 53 days old. vascular perfusion studies performed on 10 birds confirmed that the cartilaginous epiphyses of t3, t4 and t5 were essentially avascular and thus differ from those of the major long bones. findings confirm the observation that a number of morphologically distinct lesions of osteochondrosis occur in broiler fowls and that, when physeal clefts can be implicated in their aetiology, ... | 1989 | 2514211 |
| effect of gonadotropin (fsh + lh) and thyrotropin (tsh) administered with or without endotoxin at the age of three weeks on the response capacity of the thyroid gland in adult rats. | at the age of three weeks the experimental animals received either thyrotropin (tsh), or gonadotropin (fsh + lh), or endotoxin (lps) alone or in combination. the effectivity of the treatments was evaluated at the age of two months (with or without further hormone treatment). contrastingly to neonatal tsh treatment, tsh treatment at the age of three weeks did not give rise to imprinting. in female animals, however, tsh treatment increased the sensitivity to the related gonadotropin hormone. at th ... | 1989 | 2513704 |
| [dysthyroidism caused by amiodarone. frequency and diagnosis]. | the frequency of dysthyroidism during treatment with amiodarone makes it necessary to watch the thyroid gland, especially since that organ may apparently be intact in such cases. clinical observation is insufficient owing to the patients's age and to the beta-blocking effect of amiodarone. biochemical monitoring is accurate, but amiodarone-induced abnormalities without dysthyroidism (e.g. reverse t3 levels syndrome) must be taken into account. the key to the diagnosis lies in the study of free t ... | 1989 | 2512877 |
| the efficacy and toxicity of azidothymidine (azt) in the treatment of patients with aids and aids-related complex (arc): an open uncontrolled treatment study. | the efficacy and toxicity of oral azidothymidine has been studied in 145 patients with the acquired immunodeficiency syndrome (aids) or aids-related complex (arc). the median survival time of aids patients on azidothymidine was 4.5 times higher when compared to a historical aids group who had not received the drug. this result must be interpreted with caution because of changes in treatment of hiv infection and growing awareness of aids which may have led to earlier diagnosis in the group treate ... | 1989 | 2512592 |
| temperature dependence of in vitro pituitary, testis, and thyroid secretion in a turtle, pseudemys scripta. | in vitro culture was used to examine the direct actions of temperature at the level of pituitary hormone [luteinizing hormone (lh), thyrotropin (tsh), growth hormone (gh), prolactin (prl)] responses to neuropeptides and two related peripheral endocrine responses [thyroid hormone (t4) and testicular androgen secretion] to pituitary hormones (tsh and gonadotropins) in a turtle, pseudemys scripta. all these responses were fully suppressed at very low temperatures (5-6 degrees) and maximal near the ... | 1989 | 2512196 |
| congenital hypothyroidism and partial thyroid hormone unresponsiveness of the pituitary in a patient with congenital thyroxine binding albumin elevation. | we describe a girl who presented at the age of 6 weeks with cardiogenic shock due to congenital hypothyroidism (serum thyroxine (t4) less than 12 nmol/l). thyroxine replacement therapy was instituted. in spite of high total serum t4 levels, thyroid stimulating hormone (tsh) serum values remained elevated. the raised serum t4 levels were the result of congenital elevation of thyroid binding albumin (tba). toxic doses of both t4 and triiodothyronine (t3) normalized the elevated tsh levels indicati ... | 1989 | 2512164 |
| [a thyroxine-binding globulin of major biological significance in the serum of mice: demonstration and ontogenesis]. | we demonstrate in the mouse serum a hitherto unrecognized major thyroxine binding globulin (tbg), analogous to human tbg or to the recently discovered rat tbg. our demonstration is based on equilibrium dialysis, electrophoresis, immunoelectrodiffusion and autoradiography techniques. mouse tbg displays a remarkable ontogenic pattern, with 2-3 times higher activity in foetal than in maternal serum, and a further dramatic increase after birth. between 1 and 5 days, the t4 binding to serum reaches p ... | 1989 | 2512002 |
| impact of nonlethal levels of metacid-50 and carbaryl on thyroid function and cholinergic system of channa punctatus. | with regard to brain acetylcholinesterase and acetylcholine and serum triiodothyronine (t3) and thyroxine (t4) profiles, a biphasic response pattern was elicited in channa punctatus chronically exposed to nonlethal doses of locally used pesticides, namely, metacid-50 and carbaryl. data revealed that these xenobiotics caused significant inhibition of brain acetylcholinesterase activity and a decrease in thyroxine level accompanied by a concurrent increase in acetylcholine accumulation and t3 leve ... | 1989 | 2511899 |
| low basal thyrotropin with normal thyroid function in primary hyperparathyroidism. | tsh serum levels and thyroid function in 32 patients with primary hyperparathyroidism and hypercalcemia were compared to those of 30 age and sex-matched normal subjects. serum t3 and t4 concentrations in hyperparathyroidism were not different from normal. however, basal serum tsh concentrations measured with an ultrasensitive immunoradiometric assay were significantly lower than normal (1.09 +/- 0.49 vs 2.06 +/- 0.85 mu/l, p less than 0.001). in hyperparathyroidism, tsh, but not t4 or t3, was ne ... | 1989 | 2511713 |
| [energy demands and supply in polytraumatized patients in the postoperative phase]. | normal enteral feeding cannot be maintained after trauma. post-aggression metabolism or socalled "flow phase" usually results from hormonal changes, including rise in catecholamine and cortisole, drop in testosterone as well as t3 and t4, and relative hgh deficit. glucose intolerance, increased demand for energy, and catabolism are the consequences along with reduced protein uptake and increased protein breakdown with all associated complications. indirect calorimetry is an important method to d ... | 1989 | 2511703 |
| endocrine changes after burn trauma--a review. | after burn trauma, a very marked endocrine response occurs. almost all the known hormones take part in it. their response influences very much the postburn metabolic changes and participates in the integration of the body's response with the nervous and immune systems. in this review, mainly the changes in various hormone levels are described, as well as the possible role of the acute phase response after burn trauma, and the communications between the endocrine and immune systems, the cells of ... | 1989 | 2511373 |
| contributions of left-handed helical residues to the structure and stability of bacteriophage t4 lysozyme. | non-glycine residues in proteins are rarely observed to have "left-handed helical" conformations. for glycine, however, this conformation is common. to determine the contributions of left-handed helical residues to the stability of a protein, two such residues in phage t4 lysozyme, asn55 and lys124, were replaced with glycine. the mutant proteins fold normally and are fully active, showing that left-handed non-glycine residues, although rare, do not have an indispensable role in the folding of t ... | 1989 | 2511328 |
| effects of ketoacidosis and puberty on basal and trh-stimulated thyroid hormones and tsh in children with diabetes mellitus. | basal and trh-stimulated thyroid hormones and tsh were evaluated in two groups of prepubertal and pubertal diabetics: group b - 45 children without ketoacidosis; group c - 16 children with ketoacidosis. the diabetic patients showed no signs of diabetic microangiopathy. fifty-three healthy subjects served as controls (group a). t4, t3, ft4 and ft3 serum levels were reduced in diabetics, particularly in ketotic ones; t4 and t3 values were lower in pubertal than in prepubertal non-ketotic diabetics ... | 1989 | 2511132 |
| kaposi's sarcoma as clinical manifestation of the acquired immunodeficiency syndrome in a hemophilic patient. | the authors report the natural history of hiv infection in a patient with severe hemophilia a who became hiv-seropositive in 1983 and, four years later, developed full-blown aids associated with a disseminated kaposi's sarcoma. neutralizing antibody titers against hiv were shown to be inversely disease-associated, while the progression of clinical symptoms was directly related to the decline of t4 cells and the increase of urinary neopterin levels. it is suggested that the presence of an hla dr ... | 1989 | 2511103 |
| a longitudinal study on cortisol, prolactin and thyroid hormones in users of norplant subdermal implants or a copper t device. | a longitudinal study was undertaken to assess prl, cortisol, tsh, t3 and t4 levels in norplant implant users (n = 22) and copper t users (n = 20). participants were healthy women with no contraindication for hormonal contraception. samples were drawn at 0930 to 1000 h twice pretreatment and at month 2, 4, 6, 12, 24 and 36 after insertion. at the second treatment month, a second sample was obtained at 1600 h to assess cortisol diurnal variation. hormones were measured by ria. values for all hormo ... | 1989 | 2510969 |
| dietary thyrotropin-releasing hormone stimulates growth rate and increases the insulin: glucagon molar ratio of broiler chickens. | the effects of thyroid manipulation on growth, feed efficiency, and plasma hormone levels were determined in rapidly growing chickens. beginning at 3 weeks of age, eight broiler cockerels were provided with control feed (cf) or feed containing either 1 ppm of triiodothyronine (t3), 1 ppm of thyroxine (t4), 0.3% propylthiouracil (ptu), or 5 ppm of thyrotropin-releasing hormone (trh) for 3 weeks. blood samples were taken at 4, 5, and 6 weeks for determination of plasma levels of growth hormone, in ... | 1989 | 2510176 |
| single amino acid changes that alter the dna sequence specificity of the dna-[n6-adenine] methyltransferase (dam) of bacteriophage t4. | bacteriophage t4 codes for a dna-[n6-adenine] methyltransferase (dam) which recognizes primarily the sequence gatc in both cytosine- and hydroxymethylcytosine-containing dna. hypermethylating mutants, damh, exhibit a relaxation in sequence specificity, that is, they are readily able to methylate non-canonical sites. we have determined that the damh mutation produces a single amino acid change (pro126 to ser126) in a region of homology (iii) shared by three dna-adenine methyltransferases; viz, t4 ... | 1989 | 2510127 |
| effect of cranial irradiation on hypothalamus and pituitary functions. | hypopituitarism can occur after cranial irradiation for tumors distant from the pituitary gland. recent studies have suggested that this is hypothalamic in origin. hypothalamic and pituitary functions were studied in 11 patients, 4 men and 7 women, 4.5 years or more after radiotherapy for nasopharyngeal carcinomas. the estimated average total dose was 5000 cgys for the hypothalamus and pituitary gland. except for 2 women with amenorrhea and 4 men with impotency, the patients did not have evident ... | 1989 | 2509631 |
| effects of infusions of various doses of bovine growth hormone-releasing factor on blood hormones and metabolites in lactating holstein cows. | in two experiments, the effects of i.v. infusions of various doses of bovine gh-releasing factor (grf) on blood hormones and metabolites in lactating holstein cows were determined. in experiment 1, cows were infused with grf (0, 3.125, 6.25, 12.5, 25.0 or 50.0 mg/cow per 24 h) for 24 h. blood was sampled at -1, 5, 11, 15 and 23 h relative to the start of the infusion. the serum concentration of somatomedin c (sm-c) before infusion was 303 +/- 8 (s.e.m.) micrograms/l. doses of grf of between 3.12 ... | 1989 | 2509616 |
| use of the 32p-postlabelling assay to study transplacental carcinogens and transplacental carcinogenesis. | the formation of dna adducts represents a key step in the postnatal initiation of the carcinogenic process. little is known as yet about the role of prenatally induced adducts in transplacental carcinogenesis in offspring. measurement of transplacental dna damage in fetal organs of experimental animals has been difficult in the past because of the small amounts of dna available and low adduct levels. in principle, these difficulties have been overcome by the recent development of a highly sensit ... | 1989 | 2509347 |
| [clinical studies on lipid metabolism in hyperthyroidism and hypothyroidism--evaluation of serum apolipoprotein levels before and after treatment]. | the present study was undertaken to assess lipid metabolism in patients with thyroid dysfunction with special reference to serum apolipoprotein levels. serum lipid, lipoprotein and apolipoprotein levels were determined in 28 hyperthyroid and 16 hypothyroid female patients while untreated and euthyroid. apolipoproteins were measured by the method of single radial immuno-diffusion (srid). these results were compared with the values of 28 female controls. in the untreated hyperthyroid group, the se ... | 1989 | 2509259 |
| importance of spinal noradrenergic pathways in cardiovascular reflexes and central actions of clonidine and alpha-methyldopa in the rabbit. | we have examined in conscious rabbits the chronic effects of 6-hydroxydopamine (6-ohda)-induced local lesions of the spinal noradrenaline (na) pathways on (i) resting mean arterial pressure (map) and heart rate (hr), (ii) the nasopharyngeal pressor response, (iii) the sympathetic component of the baroreceptor-heart rate reflex (iv) the acute responses to intracisternal (i.c.) clonidine and alpha-methyldopa (alpha-md), and (v) the acute na release response produced by i.e. 6-ohda. one month after ... | 1989 | 2508991 |
| disparity in the response of sex steroid-binding protein and corticosteroid-binding globulin to thyroxine in the primate. | there is uncertainty regarding the differential effects of thyroid hormone on the circulating levels of sex steroid-binding protein (sbp) and corticosteroid-binding globulin (cbg). therefore, we studied the effects of elevated thyroxine (t4) on sbp and cbg concentrations in serum in 4 baboon (papio anubis) infants between 6 and 7 mo of age. the infants were given levothyroxine in increasing doses (25 to 600 micrograms) over a 1-mo period until the peripheral t4 levels were 3-4 times higher than ... | 1989 | 2508770 |
| decreased thyroidal triiodothyronine secretion in patients with anorexia nervosa: influence of weight recovery. | basal thyroxin (t4), triiodothyronine (t3), and thyrotropin (tsh) concentrations were significantly lower before weight recovery in 10 patients with anorexia nervosa (an) than they were in control subjects. after weight recovery, basal t4 and tsh levels were unchanged and significantly lower in an patients than in control subjects. basal t3 concentrations increased significantly after weight gain: however, concentrations remained lower than those in the control subjects. the maximum increase in ... | 1989 | 2508460 |
| split-course radiotherapy for urinary bladder cancer. | in this retrospective study 119 patients with t1-t4 carcinoma of the urinary bladder were treated with split-course radiotherapy. the 3-week rest period was compensated with a 10% increase in the total radiation dose to 6600 cgy. therapy was completed as planned by 86% of the patients. the actuarial 5-year survival for these patients was 20%. both the 3- and 5-year survival figures were better for patients with local control of the tumour achieved either by combined surgery and radiotherapy or b ... | 1989 | 2508191 |
| [changes in thyroid hormone concentration in liver disease]. | to evaluate the level of serum thyroxine-binding globulin (tbg) in various liver diseases, tbg and t4, t3, ft4 were measured by radioimmunoassay in 29 hbsag carriers (c), 27 patients with acute hepatitis (ah), 18 patients with inactive chronic hepatitis, 70 patients with chronic active hepatitis (cah), 31 patients with active cirrhosis (ac), 20 patients with inactive cirrhosis (ic), 38 patients with hepatocellular carcinoma (hcc), 12 patients with metastatic ca to the liver (met.) and in 81 norm ... | 1989 | 2507736 |
| deficient erythrocyte membrane gs alpha activity and resistance to trophic hormones of multiple endocrine organs in two cases of pseudohypoparathyroidism. | patients with pseudohypoparathyroidism (phpt) have been shown to have deficient cell membrane stimulatory gtp-binding regulatory protein activity (gs activity) and other defects, which cause renal resistance to parathyroid hormone (pth). those patients also have a higher incidence of deficiency of other endocrine axes. to investigate the mechanisms of this syndrome on 2 patients, we measured erythrocytic ghost gs activity by adp-ribosylation method, which showed low and low normal levels for the ... | 1989 | 2507735 |
| thyroxine may decrease serum thyroxine-binding globulin levels. | the purpose of this study was to elucidate the influence of thyroxine on the serum thyroxine-binding globulin (tbg) levels. serum tbg levels were determined in 100 normal people, 100 cases of simple goiter, 50 cases of nodular goiter, 50 cases of graves' disease and 70 cases of autoimmune thyroiditis (35 cases in the euthyroid state, and 35 cases in the hypothyroid state). the serum tbg levels were 14.3 +/- 0.3 micrograms/ml (mean +/- se) in the normal control, 14.1 +/- 0.4 micrograms/ml in simp ... | 1989 | 2507733 |
| altered thyrotropin (tsh) carbohydrate structures in hypothalamic hypothyroidism created by paraventricular nuclear lesions are corrected by in vivo tsh-releasing hormone administration. | tsh is a glycoprotein hormone composed of two subunits with attached carbohydrate chains that have varying structural characteristics. to determine the role of trh in vivo in regulating structural characteristics of tsh carbohydrate chains, adult rats received paraventricular nuclear (pvn) lesions (n = 6) or sham lesions (n = 6). the pvn contain large amounts of trh, and rats with lesions in these hypothalamic nuclei have been shown to have decreased plasma thyroid hormone levels. at 10 days aft ... | 1989 | 2507289 |
| measurement and clinical significance of thyroid microsomal and thyroglobulin antibodies by enzyme-linked immunosorbent assay. | using the thyroid microsomal antigen(tmag) prepared by affinity chromatography gel filtration method of sufficient purity, we measured the tm antibody (tmab) level by an enzyme-linked immunosorbent assay (elisa) in 103 normal persons and 183 patients with various thyroid disorders (hashimoto's thyroiditis, graves' disease, hypothyroidism, subacute thyroiditis, thyroid cancer, thyroid adenoma and simple goiter). the thyroglobulin antibody (tcab). t3 and t4 were also measured at the same time. bas ... | 1989 | 2507242 |
| decreased serum tbg concentration in the face of normal t4-binding capacity in patients on hemodialysis. | serum tbg concentrations were evaluated in 34 patients on regular hemodialysis. mean serum tbg level measured by two different radioimmunoassays was markedly decreased (1.24 +/- 0.08 mg/dl - normal limits 1.6-2.4 mg/dl). twenty-seven (79%) patients had values below 1.6 mg/dl, which contrasted in 21 of them with a lack of reciprocal elevation of t3ru values. by contrast, t4-binding capacity of tbg measured in 14 patients with low tbg concentration was normal, suggesting that the low tbg values we ... | 1989 | 2507207 |
| flow cytometric analysis of dna content of deparaffinized nuclei in urinary bladder carcinomas. comparison of different isolation methods and relation to histological grade and stage. | a consecutive series of 230 patients with transitional cell carcinoma of the urinary bladder was analyzed with respect to the dna content of the primary tumor, as determined by flow cytometry on material obtained from paraffin blocks. a new method for this analysis was elaborated, based on proteolytic digestion with protease (type vii, sigma) and density separation of the nuclei by percoll centrifugation. the method provided dna flow histograms with less baseline debris and a smaller coefficient ... | 1989 | 2506919 |
| nucleotide sequence of the alt gene of bacteriophage t4. | 1989 | 2506526 | |
| thyroid hormone regulation of neurons staining for a pro-trh-derived cryptic peptide sequence in the rat hypothalamic paraventricular nucleus. | the influence of thyroid hormones on neurons staining for a pro-trh-derived cryptic peptide sequence in the rat hypothalamic paraventricular nucleus was assessed by using quantitative immunocytochemistry. the antiserum used for immunocytochemical studies recognized a cryptic fragment of pro-trh located in the phe 178-ser 186 region of the precursor. hypothyroid female rats obtained following 1 month administration of 6-n-propyl-2-thiouracil (ptu) as well as euthyroid animals were treated with a ... | 1989 | 2506483 |
| mechanism of the serum thyroid hormone lowering effect of perfluoro-n-decanoic acid (pfda) in rats. | the mechanism and consequences of the serum thyroid hormone lowering effect of perfluorodecanoic acid (pfda) were examined. thyroid and pituitary gland functions in pfda-treated rats were assessed by measuring radioiodine uptake from the circulation and the ability of the thyroid gland to secrete thyroxine (t4) and triiodothyronine (t3) in response to thyrotropin-releasing hormone (trh) stimulation. serum levels of reverse triiodothyronine (rt3) were measured to test for possible conversion of t ... | 1989 | 2506363 |
| the pituitary-thyroid axis in patients with pituitary disorders. | the pituitary-thyroid axis of 12 patients, exposed to transsphenoidal pituitary microsurgery because of nonfunctioning adenomas (6), prolactinomas (3) and craniopharyngioma (1), or to major pituitary injury (1 apoplexy, 1 accidental injury), was controlled more than 6 months following the incidents. the patients did not receive thyroid replacement therapy and were evaluated by measurement of the serum concentration of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), ... | 1989 | 2506120 |
| thyroid hormone binding in early pregnancy and the risk of spontaneous abortion. | serum thyroxine (t4), free thyroxine (ft4) and thyroxine binding globulin (tbg) were measured at 6, 8, 10, 12, 14 and 16 weeks menstrual age (ma) in 32 pregnant females, who had been selected for a high risk of spontaneous abortion on account of previous abortion (18 cases) or ovulation induction (14 cases). eight of these subjects experienced a spontaneous abortion occurring between 8 and 20 weeks. the other women, who carried to term, served as controls. in the abortion group, mean pre-abortio ... | 1989 | 2506086 |
| screening of congenital hypothyroidism in newborns in bohemia and moravia. | regular examinations of thyroid function in newborn infants were started in the district of praha 10 in 1975. later the incidence of congenital hypothyroidism in czechoslovakia was assessed in a pilot study including several centers in 1981. as a screening method we used the estimation of total thyroxine (t4) in a dried spot of blood obtained from a heel prick on the fifth day after birth. a total of 45,535 newborns was screened by such method and the incidence was 1:5059 of live born infants. s ... | 1989 | 2506000 |
| low-normal concentrations of free thyroxin in serum in late pregnancy: physiological fact, not technical artefact. | free thyroxin (ft4) concentrations, total thyroxin/thyroxin-binding globulin (t4/tbg) ratios, and thyrotropin (tsh) and albumin concentrations were measured in serum in a longitudinal study in each of the three trimesters of 25 normal pregnancies. in late pregnancy, ft4 estimates by assays reputedly either affected or unaffected by albumin were in the lower half of the reference range for nonpregnant subjects. t4/tbg ratios and albumin concentrations were similarly lower. ft4 overall was signifi ... | 1989 | 2505949 |
| thyroxine-binding globulin and thyroxine-binding prealbumin in hypothyroid and hyperthyroid developing rats. | we present evidence based on equilibrium and non-equilibrium binding studies, as well as on immunological techniques, that of the two rat specific thyroid-hormone-binding proteins, i.e., thyroxine-binding globulin (tbg) and thyroxine-binding prealbumin (tbpa), tbg but not tbpa is regulated by the thyroid hormones (th). hypothyroidism, induced from the day of birth by daily treatment with propylthiouracil (ptu-rats), leads to dramatic and sustained increases of the th-binding abilities of the ser ... | 1989 | 2505856 |
| binding of adenosine diphosphoribosyltransferase to the termini and internal regions of linear dnas. | binding mechanisms of adpr-transferase to restricted double-stranded dna fragments of sv40 and pbr322 dna were determined by nuclease protection techniques. top and bottom strands of double-stranded dna were identified by specific labeling with 32p. protection against specific exonucleases identified binding of adpr-transferase to dna termini, whereas binding to internal regions of linear dnas was probed by protection against endonucleases. adpr-transferase protein protected against exonucleolyt ... | 1989 | 2505840 |
| changes in basal and stimulated tsh and other parameters of thyroid function in acromegaly after transsphenoidal surgery. | t1 and t3 levels, tsh response to trh and somatomedin-c levels in 63 patients with acromegaly, were measured before transsphenoidal surgery and during a 4-year follow-up period. criteria for cure were: mean gh level less than 5 mu/l, suppression of gh by oral glucose tolerance test below 2.5 mu/l and normalization of paradoxical gh reaction to trh. nine patients underwent radioiodine studies to assess the renal and thyroid clearance of iodide, plasma inorganic iodine level and absolute iodine up ... | 1989 | 2505466 |
| biochemical and serological properties of actinobacillus pleuropneumoniae biotype 2 strains isolated from swine. | the biochemical and serological properties of 21 strains of actinobacillus pleuropneumoniae biotype 2 isolated from haemorrhagic necrotic pleuropneumonia of swine were examined. for serologic typing, the indirect haemagglutination (iha) and the double gel-diffusion tests were used. on the basis of their soluble surface antigens, our a. pleuropneumoniae biotype 2 isolates could be assigned to two proposed serotypes. serotype 1 comprised 11 strains and serotype 2 comprised 10 strains. all strains ... | 1989 | 2505435 |
| identification and characterization of the genes encoding three structural proteins of the thermoproteus tenax virus ttv1. | three structural proteins, tp1, tp2 and tp3, of the virus ttv1 of the thermophilic archaebacterium thermoproteus tenax strain kra1 were mapped within the viral genome by locating the amino-terminal amino acid sequences of these proteins in the ttv1 dna sequence. the derived amino acid sequences comprise 113, 139 and 160 amino acid residues, respectively. all three proteins are hydrophobic. the three genes are not linked, but transcribed in the same direction. no shine-dalgarno sequences are foun ... | 1989 | 2505050 |
| serum thyroid hormone levels in liver cirrhosis. | levels of thyroid hormones, serum thyroxine, triiodothyronine (t3), free thyroxine (ft4), free triiodothyronine (ft3) and thyrotropin (tsh) were measured in 55 patients with liver cirrhosis using radioimmunoassay techniques. results were compared with 78 controls. the mean serum concentration of t3, ft3 and ft4 were significantly decreased in cirrhotics, while no significant change was noted in serum t4 and tsh levels. t3/t4 ratio was also lower than the normal. this indicates an impaired liver ... | 1989 | 2504964 |