Publications

pharmacokinetics of 3,5,3'-triiodothyroacetic acid and its effects on serum tsh levels.	3,5,3'-triiodothyroacetic acid is an effective inhibitor of tsh secretion in central hyperthyroidism. serum, 3,5,3'-triiodothyroacetic acid was measured with an ria preceded by immunoprecipitation. an anti-3,5,3'-triiodothyroacetic acid antibody was obtained in rabbits, using 3,5,3'-triiodothyroacetic acid coupled to hemocyanin and diazotized benzidine as antigen (cross-reactivity with t4, t3, tetraiodothyroacetic acid was 0.2, 1.1, and 5%, respectively). endogenous 3,5,3'-triiodothyroacetic aci ...	1989	2588936
[low t3 syndrome and chronic inflammatory rheumatism].	for the clarification of pathogenesis and clinical relevance of decreases of the triiodothyronine (t3) level in patients with chronic inflammatory rheumatism in a group of 63 patients with clinically, paraclinically and roentgenologically diagnosed rheumatoid arthritis (59 times) and with sle (4 times), respectively, parallel were determined parameters of the thyroid gland function and of the rheumatic activity as well as a subtile drug anamnesis for the medication of antirheumatic drugs was est ...	1989	2588721
plasma free thyroxine concentrations in patients receiving levothyroxine for thyroid suppression.	the usual recommendation is to follow total triiodothyronine (t3) concentration during levothyroxine (l-thyroxine) therapy because 65% of clinically euthyroid patients receiving l-thyroxine have normal t3 but elevated total thyroxine (t4) levels. because free thyroxine (ft4) is the metabolically active form of t4, our study was designed to determine whether ft4 by analog radioimmunoassay method is normal or increased in euthyroid patients receiving l-thyroxine. twenty-seven clinically euthyroid ...	1989	2588121
turkey plasma thyroid hormone and prolactin concentrations throughout an egg laying cycle and in relation to photorefractoriness.	plasma concentrations of thyroxine (t4), triiodothyronine (t3), and prolactin were determined weekly through an egg laying cycle induced with 30 wk of 14 h light:10 h dark. egg production, primary remige molting, and occurrence of photorefractoriness were monitored. hens were assigned to one of two groups; those that exhibited photorefractoriness (pr) and those that remained photosensitive (ps). weekly plasma t4 concentrations relative to the time of initiation of photostimulation did not differ ...	1989	2587476
effect of dietary thyroxine on growth and muscle protein metabolism in broiler chickens.	the effects of thyroxine (t4) on growth, feed efficiency, breast muscle (musculus pectoralis profundus) weight, liver weight, abdominal fat content, and rates of muscle protein synthesis (ks) and breakdown (kd) estimated by n tau-methylhistidine excretion were investigated in broiler chickens of both sexes. dietary inclusion of .4 ppm t4 resulted in a less abdominal fat content in female chickens and a higher muscle protein synthesis rate in male chickens. administration of t4 at the level of 1. ...	1989	2587475
nucleotide and deduced amino acid sequences of bacteriophage t4 gene 22.		1989	2587226
[respiratory effects of epidural anesthesia and anesthetic level obtained in patients for cesarean section].	in order to determine the optimal level of epidural anesthesia, the author examined respiratory function of 138 full-term pregnant women in sitting and supine positions without anesthesia, and in supine position under epidural anesthesia prior to the cesarean section. vital capacity (vc) increased in the sitting position in comparison with supine position because expiratory reserve volume (erv) and tidal volume (tv) increased. in contrast, %ffv1.0, peak expiratory flow (pef) and v75 decreased as ...	1989	2585698
orientation of the bases of single-stranded dna and polynucleotides in complexes formed with the gene 32 protein of bacteriophage t4. a linear dichroism study.	linear dichroism measurements were performed in the wavelength region 250 to 350 nm on complexes between the single-stranded dna binding protein of bacteriophage t4 (gp32) and single-stranded dna and a variety of homopolynucleotides in compressed polyacrylamide gels. the complexes appeared to orient well, giving rise to linear dichroism spectra that showed contributions from both the protein aromatic residues and the bases of the polynucleotides. in most cases the protein contribution appeared t ...	1989	2585494
pre-irradiation chemotherapy for infants and children with medulloblastoma: a preliminary report.	from march, 1984, through june, 1987, 21 newly diagnosed children with high-risk medulloblastoma (chang stage t3 to t4) were treated on a 9-week postoperative, pre-irradiation chemotherapy regimen consisting of vincristine and cisplatin. the children over 2 years old then received radiation therapy. six infants (aged 6 to 18 months) were maintained on chemotherapy consisting of mop (nitrogen mustard, vincristine, and procarbazine) until the age of 2 years, at which time they were referred for ir ...	1989	2585072
perforin and serine esterase gene expression in stimulated human t cells. kinetics, mitogen requirements, and effects of cyclosporin a.	a pore-forming protein (pfp; perforin) and various serine esterases (se) have been identified in the cytoplasmic granules of ctl and nk cells. perforin and several se have recently been cloned. northern blotting analysis was performed here using cdna probes specific for human perforin and two se (se 1/hs and se 2/gb) to monitor the levels of specific mrnas in mitogen-stimulated primary human t cells. these mrnas were rapidly induced by il-2 with optimal responses at 300 u/ml. after il-2 treatmen ...	1989	2584937
low levels of thyroglobulin messenger ribonucleic acid in congenital goitrous hypothyroidism with defective thyroglobulin synthesis.	we characterized the virtual absence of immunoassayable thyroglobulin (tg) in the serum and thyroid gland of two siblings (ma, jna) and one nephew (rss) from a family without inbreeding or familial goiter. diagnosis of defective tg gene expression was based on findings of normal pbi and low serum t4, low or normal serum t3, negative perchlorate discharge test, and virtual absence of the serum tg response to challenge by bovine tsh. this conclusion was confirmed by analysis of proteins in the goi ...	1989	2584351
[relevance of the new tumor marker scc (squamous cell carcinoma antigen) for the diagnosis and follow-up control of squamous epithelial carcinoma of the head and neck].	the scc antigen, a tumour marker for squamous cell carcinoma, is already used for the diagnosis and follow-up of carcinoma of the cervix and the lungs. we determined the scc antigen levels at the time of diagnosis and during therapy in 108 subjects with a squamous cell carcinoma of the head and neck. according to our results and those of other authors, the normal serum range of scc lies between 0 and 2 ng/ml. before therapy we found an increased titre in 38.9% of the subjects, being 6.2%, 30.8%, ...	1989	2584067
use of sensitive thyrotrophin measurements in an elderly population.	the investigation of thyroid disease in the elderly is difficult. conventional tests are influenced by ill health and abnormal concentrations of thyroid binding proteins. to screen for thyroid disease thyroxine (t4), then tri-iodothyronine (t3) or tsh and an index for binding proteins are normally measured, methods which are expensive and time-consuming. tsh immunoradiometric assays (irma) offer the potential of a single test assessment of thyroid function in the elderly. this was assessed by co ...	1989	2583535
an mboii/foki trimming plasmid allowing consecutive cycles of precise 1- to 12-base-pair deletions in cloned dna.	a novel trimming plasmid has been designed which allows, in a preprogrammed fashion, the precise deletion of up to 12 bp per cleavage cycle, from one end of a cloned fragment. the plasmid, which carries the dhfr gene, contains unique recognition sites for two class-iis restriction enzymes, mboii and foki, which are arranged in the form of a cassette, so that consecutive cleavages with these endonucleases, followed by blunting with mung bean nuclease (mb), will precisely delete 12 bp of adjacent ...	1989	2583524
changes in tissue and blood concentrations of thyroid hormones in developing chum salmon.	the changes in tissue and blood concentrations of thyroxine (t4) and triiodothyronine (t3) were examined during development of the chum salmon (oncorhynchus keta). extraction methods previously established for tissue t4 were also validated for tissue t3, by parallel displacement curves to t3 standard in the radioimmunoassay and by the same elution patterns of immunoreactivity in a hplc system. the t3 concentration of the eggs just after fertilization (4-9 ng/g) was lower than the t4 concentratio ...	1989	2583473
[fluctuations in the titers of anti-thyroid hormone and anti-thyroglobulin antibodies in 4 cases of graves' disease during long-term treatment period].	we studied 4 cases of graves' disease with anti-thyroid hormone antibodies. changes in the serum levels of triiodothyronine(t3), thyroxine(t4), free t4, thyrotropin(tsh), and thyroglobulin(tg), as well as titers of anti-tg antibodies, anti-thyroid hormone antibodies, anti-tsh receptor antibodies(trab) and anti-microsomal antibodies(mcha) during 2 10 years' treatment periods were examined in each case. case 1; a woman, who was diagnosed as having graves' disease when she was 10 years old, had bee ...	1989	2583311
[changes in anti-thyroid hormone and anti-thyroglobulin antibodies during thyroid hormone(s) or prednisolone treatments in 3 cases of hashimoto's thyroiditis].	we have experienced 3 cases of hashimoto's thyroiditis with anti-thyroid hormone antibodies. changes in titers of anti-thyroglobulin(tg) and anti-thyroid hormone antibodies during 6 year (cases 1 and 2) and 7 year (case 3) observation periods were examined in each case. cases 1 (13-year-old) and 2 (10-year-old) are sisters with hypothyroidism whose chief complaint was short stature. they were diagnosed as having hashimoto's thyroiditis by needle biopsy of the thyroid gland. presence of anti-thyr ...	1989	2583310
the scute (t4) gene acts as a numerator element of the x:a signal that determines the state of activity of sex-lethal in drosophila.	the ratio of x chromosomes to sets of autosomes (x:a) is the primary genetic signal that determines sex and dosage compensation in drosophila. the gene sex-lethal (sxl) receives this signal and is responsible for the execution of the alternative developmental programmes of males and females. we have found that the scute (t4) gene, which is involved in neurogenesis, also plays a role in the activation of sxl. the following results suggest that scute (t4) may be a numerator element of the x:a sign ...	1989	2583094
analysis of overall pathophysiological relationships between serum levels of tsh and thyroid hormones using a large laboratory database.	factors associated with the basal level of serum thyrotropin (tsh) were analyzed over a wide range of pathophysiological conditions by means of a large laboratory database on thyroid function. when data were analyzed two-dimensionally, serum tsh showed significant inverse correlations with total triiodothyronine (t3), free t3 index (ft3i), total thyroxin (tt4) and free t4 index (ft4i) in the order of increasing intensity. the three-dimensional analysis, however, revealed that 1) total hormone le ...	1989	2583056
thyroid hormone and growth hormone interact to regulate insulin-like growth factor-i messenger ribonucleic acid and circulating levels in the rat.	thyroid hormones influence growth in part by altering the secretion and effects of gh. gh, in turn, mediates its effects by regulating the synthesis and secretion of insulin-like growth factor-i (igf-i). igf-i is a pleiotropic growth factor that is synthesized by many tissues and acts on many tissues to regulate both cellular replication and differentiated function. we have studied the direct effects of thyroid hormones and the combined effects of thyroid hormones and gh on the regulation of igf ...	1989	2583046
analysis of cellular populations in peritoneal effluents of children on capd.	the characterization of cellular populations in peritoneal effluents (pe) of capd patients has been the subject of few studies. in order to establish a possible correlation between pe cells and clinical parameters, we studied the immunocompetent cells that are found in uncomplicated patients. in this study we used cytofluorimetric analysis to characterize phenotypically immunocompetent pe cells. we studied 17 children with a mean age of 8.7 +/- 5.3 years, who had been on capd for a mean duration ...	1989	2582649
chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.	biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. the 5'-biotinylated oligonucleotides were enzymatically coupled to other dna fragments by t4 dna ligase or t4 rna ligase. the hybridization properties of such biotin-labeled oligonucleotide probes were studied.	1985	2582352
systemic lupus erythematosus with deficiency of the t4 epitope on t helper/inducer cells.	three black jamaicans with systemic lupus erythematosus (sle) were identified whose t helper/inducer cells lacked the t4 epitope (t4 epitope-deficient phenotype). all three patients had lymphadenopathy as part of their syndromes. the asymptomatic and otherwise healthy t4 epitope-deficient brother of one of these patients also had lymphadenopathy in a distribution identical to that of his sister with sle. family studies pointed to an autosomal codominant mode of inheritance not linked to the hla ...	1985	2582253
t3 monoclonal antibody activation of nonspecific cytolysis: a mechanism of ctl inhibition.	the t3 antigen is expressed on all cytotoxic t lymphocytes (ctl). monoclonal antibodies (mab) to the t3 antigen previously have been shown to inhibit ctl-mediated killing of cells expressing the relevant target antigens. the mechanism of t3 mab inhibition, however, remains undefined. in this report, we describe a novel effect of the t3 mab: the stimulation of allospecific ctl clones to kill target cells that do not express the relevant hla antigens. the stimulation of nonspecific killing was see ...	1985	2582036
dna primase-dna polymerase alpha from simian cells. modulation of rna primer synthesis by ribonucleoside triphosphates.	dna primase-dna polymerase alpha, purified 53,000-fold from cv-1 cells, synthesized predominantly (p)ppa(pa)6-primed dna on a poly(dt) template. about 80% of the rna primers synthesized on an m13 dna template were (p)ppa/g(pn)5-7, and 20% were (p)ppa/g(pn)0-4. rna primer size was determined by gel electrophoresis after removing nascent dna with phage t4 dna polymerase 3'-5' exonuclease, leaving a single dnmp at the 3'-end of the rna primer, and the terminal 5'-(p)ppn residue was determined by "c ...	1985	2581949
polyadenylated, noncapped rna from the archaebacterium methanococcus vannielii.	polyadenylated [poly(a)+] rna molecules have been isolated from methanococcus vannielii by oligodeoxythymidylate-cellulose affinity chromatography at 4 degrees c. approximately 16% of the label in rna isolated from cultures allowed to incorporate [3h]uridine for 3 min at 37 degrees c was poly(a)+ rna. in contrast, less than 1% of the radioactivity in rna labeled over a period of several generations was contained in poly(a)+ rna molecules. electrophoretic separation of poly(a)+ rna molecules show ...	1985	2581934
properties of a dna repair endonuclease from mouse plasmacytoma cells.	the properties of a dna-repair endonuclease isolated from mouse plasmacytoma cells have been further studied. it acted on ultraviolet-light-irradiated supercoiled dna, and the requirement for a supercoiled substrate was absolute at ultraviolet light doses below 1.5 kj m-2. at higher doses relaxed dna could also serve as a substrate, but the activity on this dna was due mostly to hydrolysis of ultraviolet-light-induced apurinic/apyrimidinic (ap) sites by the ap-endonuclease activity associated wi ...	1985	2581776
effects of antithyroid drug therapy on blood glucose, serum insulin, and insulin binding to red blood cells in hyperthyroid patients of different ages.	the mechanism of glucose intolerance in thyrotoxicosis was investigated in 119 patients with graves's disease with careful consideration of the age-related deterioration of glucose tolerance. before and after treatment of thyrotoxicosis with antithyroid drug, changes of blood glucose (bg) and serum immunoreactive insulin (iri) in response to 50 g oral glucose tolerance test (ogtt) and insulin binding to red blood cell (rbc) were evaluated. in control subjects, the sigma iri/sigma bg ratio after ...	1985	2581747
combination chemotherapy with high-dose methotrexate, bleomycin, and cisplatin in management of head and neck squamous cell carcinoma.	fifty-nine patients with stage iv head and neck squamous cell cancer were treated with an intensive induction chemotherapy consisting of high-dose methotrexate-leucovorin, bleomycin, and cisplatin. forty-five patients had recurrent disease following surgery and/or radiation therapy. the response rate in this group was 22%, with a median response duration of 10 weeks and a median survival of 19 weeks. the median survival in responders was 20 weeks and in nonresponders 18 weeks. fourteen previousl ...	1985	2581436
changes in cardiac function following administration of thyroid hormones in thyroidectomised rats: assessment using the isolated working rat heart preparation.	we investigated the changes in cardiac function following alterations in thyroid state of rats, using the isolated working heart preparation. this technique allows heart rate, contractility, and pump function to be studied under controlled conditions. surgical thyroidectomy resulted in significant impairment of cardiac function. single doses of 3,5,3'-triiodo-l-thyronine (t3) or l-thyroxine (t4) were given to thyroidectomised rats. cardiac function was restored to normal within 48 h of administr ...	1985	2581083
human t cell leukemia/lymphoma virus-infected antigen-specific t cell clones: indiscriminant helper function and lymphokine production.	the ability of human t cell leukemia/lymphoma virus (htlv)-i to alter the function of infected t lymphocytes was examined directly by investigating the properties of an antigen-specific t cell clone before and after transformation with htlv-i. following infection, the t4 antigen-specific clone manifested a tenfold increase in its surface interleukin 2 (il 2) receptor (tac) density and acquired the viral determinants p19, p24, and 4d12 not present in the uninfected clone. prior to infection, the ...	1985	2580910
characterization of the human newborn response to herpesvirus antigen.	an investigation was made into the human newborn cellular response to herpes simplex virus type 1 (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv) to understand more about the nature of the neonate's susceptibility to overwhelming infection by these viruses. newborn mononuclear cells sustained the proliferation in culture of maternal (i.e., haplotype-matched) t cell blasts with specificity for hsv, cmv, or vzv (p less than 0.05). this is evidence for intact antigen-processing capab ...	1985	2580908
rna splicing in prokaryotes: bacteriophage t4 leads the way.		1985	2580641
immunohistology of human thymomas.	we analyzed lymphocyte surface markers in seven thymomas. in six mixed thymomas, the staining patterns were similar for t11, okt8, okt6, okt4, coulter clone t4, and leu 3a/3b. staining patterns for ia and keratin showed a dendritic pattern. occasional s 100-positive interdigitating dendritic cells were identified. in three patients with associated myasthenia gravis, no significant differences in staining patterns were identified. a different pattern was seen in a patient with hypogammaglobulinem ...	1985	2580505
susceptibility of cytotoxic t lymphocyte (ctl) clones to inhibition by anti-t3 and anti-t4 (but not anti-lfa-1) monoclonal antibodies varies with the "avidity" of ctl-target interaction.	to explore the role of the t3, t4, and lfa-1 molecules in high and low "avidity" interactions between sb2-specific cytotoxic t lymphocyte (ctl) clones and their targets, monoclonal antibody-mediated inhibition of cytotoxicity has been studied in experiments that vary the "avidity" of interaction in three different ways. 1) previous results have been extended with respect to different ctl clones assayed on the same sb2-positive target cells. differences between clones in susceptibility to anti-t3 ...	1985	2580011
clinical and immunologic response to isoprinosine in alopecia areata and alopecia universalis: association with autoantibodies.	twenty patients with alopecia universalis, alopecia semiuniversalis and alopecia areata were studied for their immune parameters. fourteen of them received an oral treatment with isoprinosine, a synthetic immunomodulator. ten patients showed the presence of several autoantibodies. no significant abnormalities in various t cell rosette markers were found, but t4/t8 ratios tended to be elevated. erythrocyte antibody complement (eac) rosettes were usually decreased. treatment with isoprinosine prod ...	1985	2579988
interaction of furosemide with serum thyroxine-binding sites: in vivo and in vitro studies and comparison with other inhibitors.	the diuretic furosemide inhibits serum protein binding of t4 in equilibrium dialysis, dextran-charcoal, and competitive ligand binding separation systems and displaces [125i]t4 from isolated preparations of t4-binding globulin (tbg), prealbumin, and albumin. equilibrium dialysis studies of undiluted normal serum showed that about 10 micrograms/ml furosemide increased the free t4 and free t3 fractions. displacement occurred at lower drug concentrations in sera with subnormal albumin and tbg level ...	1985	2579968
separation and characterization of a subset of human t8+ cells which function as antigen-presenting and contrasuppressor cells.	the lectin vicia villosa (vv) binds preferentially a subset of t cells. separated vv-adherent cells (ac) do not generate helper function alone, but when added to vv-non-adherent cells (nac), they can present streptococcal antigen (sa) and induce helper function as effectively as monocytes, at a ratio of 1 vv-ac to 4 vv-nac. further separation into a t8+ subset of vv-ac and reconstitution with t4+ helper cells (hc) has established that the t8+ vv-ac induces t4+ cells to helper activity. however, ...	1985	2579897
structural invariance of t4 molecules from t cell clones of different antigen and major histocompatibility complex specificities.	recent studies have suggested that the t4 molecule may bind to class ii major histocompatibility complex (mhc) gene products on target cells. such an interaction could be with either a polymorphic or monomorphic determinant on class ii mhc genes. the first would imply that t4 is structurally variable and represents a component of a dual receptor, whereas the latter would suggest that t4 is structurally invariant and serves as an accessory binding molecule. to resolve this question we studied the ...	1985	2579824
protective effect of thyroxine but not parathyroidectomy on gentamicin nephrotoxicity.	gentamicin nephrotoxicity increases renal cortex calcium and sodium and decreases renal cortex na-k-atpase activity. human acute renal failure is accompanied by an increase in parathyroid hormone (pth), a hormone that stimulates calcium uptake by tissues, and by a decrease in thyroid hormone, a hormone that increases renal cortex na-k-atpase activity. this study evaluated the role of extracellular calcium, pth, and thyroxine in the pathogenesis of gentamicin nephrotoxicity. chronically parathyro ...	1985	2579582
interaction of triiodothyronine-receptor complexes with simian virus 40 minichromosomes in monkey kidney cv-1 cells.	thyroid hormone-responsive tissues contain chromatin-localized receptors that bind to dna and may associate preferentially with actively transcribed chromatin. to study such receptor-chromatin localization, we have used cultured cv-1 cells permissive for simian virus 40 (sv40), in which viral minichromosomes can be separated from the cellular chromatin. cv-1 cells were found to contain intranuclear thyroid hormone-binding sites with an affinity for t3 and t4 and a site concentration similar to t ...	1985	2578953
regulation of the alpha and thyrotropin beta-subunit messenger ribonucleic acids by thyroid hormones.	we studied the regulation of mrnas encoding the alpha- and beta-subunits of tsh by thyroid hormones (t4 and t3) in mouse thyrotropic tumors and pituitary glands. hypothyroid male (laf1) mice bearing thyrotropic tumor (ttt97) were injected daily with t4 for 0, 1, 5, 12, or 33 days. after day 33, plasma levels of tsh and free (unassociated) tsh beta-subunit were reduced to less than 1% of control levels, whereas free alpha-subunit was reduced to 6% of control levels. steady state levels of subunit ...	1985	2578951
bacteriophage t4 unf (=alc) gene function is required for late replication in the presence of plasmid pr386.	the bacteriophage t4 unf gene, known to be involved in the arrest of transcription from cytosine-containing dna, is unessential except in escherichia coli strains containing plasmid pr386. comparative genetic and biochemical analyses of parameters of unf+ and unf- phage growth in host cells isogenic except for the presence or absence of plasmid pr386 have shown that unf gene function is required for late phage dna synthesis in the presence of the plasmid. shutoff of host dna, rna, and protein sy ...	1985	2578570
pregnancy-associated growth factor. ii. a t-dependent polyclonal activator of human adult peripheral blood lymphocytes (pbl).	this study examined the ability of pregnancy-associated growth factor (pagf), a substance found in crude human chorionic gonadotropin (hcg), to induce plaque-forming cells (pfc) in cultured human peripheral blood lymphocytes (pbl). pagf, 0.25 to 1 mg/ml, induced maximal pfc at 6 to 7 days as measured by the staphylococcal protein a-coupled srbc reverse hemolytic plaque assay with a rabbit anti-human ig antiserum. pagf-induced pfc/culture ranged from 1800 to 39,000 with a mean of 11,524 in unfrac ...	1985	2578516
hydrodynamic studies of a dna-protein complex. elongation of single stranded nucleic acids upon complexation with the gene 32 protein of phage t4 deduced from electric field-induced birefringence experiments.	short dna and rna fragments complexed with the helix destabilizing protein of bacteriophage t4, gp32, have been studied in solution by electric birefringence and circular dichroism. the birefringence of the complexes is positive and the magnitude indicates that the dna and rna fragments become linear and rigid upon protein binding. the field free decay is biphasic. on the basis of a rigid rod approximation the slow relaxation time leads to a base-base distance along the helix axis in the complex ...	1985	2578411
purification of rna polymerase and transcription-termination factor rho from erwinia carotovora.	erwinia carotovora rna polymerase consists of the holoenzyme structure sigma 2 beta beta' sigma as found in escherichia coli and other bacteria. e. carotovora rna polymerase can synthesize rna using lambda, t7 of t4 dna as templates; however, it is two times less active on these templates and is more temperature-sensitive than the e. coli enzyme. the alpha subunit of the e.. carotovora enzyme is lower in molecular mass than its e. coli counterpart. the sigma factors from e. coli and e. carotovor ...	1985	2578393
surface antigen specificity of cold-reactive igm antilymphocyte antibodies in systemic lupus erythematosus.	monoclonal antibodies to known surface antigens on b cells and on resting and activated t cells of various types were used in several approaches to examine the specificity of igm antilymphocyte antibodies in systemic lupus erythematosus (sle). surface determinants that were sought included: t3, t11, leu-1, leu-8 (pan-t); t4, t8 (t subset); beta 2-microglobulin (beta 2m); l243, leu-10 (dr and ds/dc framework, respectively); anti-tac (interleukin-2 receptor); 5e9 (transferrin receptor); and 4f2, a ...	1985	2578282
impaired in vitro interferon, blastogenic, and natural killer cell responses to viral stimulation in acquired immune deficiency syndrome.	the in vitro immune response to herpes simplex virus (hsv), type 1, strain 539, hsv type 2, strain 316d, and cytomegalovirus was studied in 20 patients (14 with acquired immune deficiency syndrome, four with the acquired immune deficiency syndrome-related symptom complex, and two sexually active asymptomatic homosexuals) and 18 heterosexual healthy controls. peripheral blood mononuclear cells were cultured with 2 x 10(5) plaque-forming units of heat-inactivated viruses, their lymphocyte blastoge ...	1985	2578099
improved genomic blot hybridization.	for restriction fragment length polymorphism (rflp) and other molecular biology experiments, well prepared (undamaged) genomic dna is needed, which is then digested with restriction enzymes, transferred to nitrocellulose paper and hybridized with radioactive or nonradioactive probe. dna damage occurs in fields such as clinical genetics and forensic medicine in the form, for example, of single stranded breaks. if these dnas are treated with t4 dna ligase prior to restriction endonuclease digestio ...	1989	2576631
complement-dependent cytotoxicity of antibodies reactive with hiv-induced cell surface antigens in hiv-carrying haemophiliacs.	sera obtained from hiv-infected as well as uninfected haemophiliacs and from healthy subjects were investigated for the presence of lymphocytotoxic antibodies. using the 51cr-release test, hiv-infected haemophiliacs were found to produce serum antibodies exerting complement-dependent cytotoxic effect on hiv-infected t4 cells. the antibodies were reactive mainly when hiv-infected target cells were stimulated with concanavalin-a. results of complement-dependent antibody cytotoxicity and indirect m ...	1989	2576594
[novel immunotherapeutic models for neoplastic diseases of the urogenital tract with special attention to bladder carcinoma].	in this review article some novel immunotherapeutic models as well as some new concepts concerning the unspecific and specific t-cell stimulation are discussed briefly. some of these immunotherapeutic models are restricted to bladder cancer; the others have a generalized meaning. one model, restricted to bladder cancer, is based on the enzymatic++ pretreatment of effector-cells (macrophages, nk-cells), followed by their instillation in the patient's bladder. the other bladder tumor restricted mo ...	1989	2576184
does t4 lymphocyte depletion predispose to hiv infection?		1989	2576020
effects of beta-adrenergic blockers with different ancillary properties on lipid peroxidation in hyperthyroid rat cardiac muscle.	to determine whether beta-blockade protects rat heart against thyroxine (t4)-induced accelelation of lipid peroxidation, in vivo effects of 3 beta-blockers with different ancillary properties on the mitochondrial oxidative enzyme, antioxidant enzymes and lipid peroxide were investigated. the rats were rendered hyperthyroid by adding t4 to their drinking water for 3 weeks and were treated simultaneously with either carteolol (a blocker with partial agonist activity; 30 mg/kg/day), atenolol (50 mg ...	1989	2575990
[sufentanil: the effect on cardiocirculatory parameters and intubation conditions on administration of pancuronium or vecuronium].	a lack of uniform methodology used in the assessment of moderate doses of sufentanil in combination with non-depolarizing neuromuscular blocking drugs formed the basis of the current study which compared under randomized conditions the effects of sufentanil-pancuronium versus sufentanil-vecuronium on hemodynamics, intubating conditions and chest wall rigidity during induction of anesthesia. material and methods. one hundred and twenty asa physical status i and ii patients aged between 20 and 40 ...	1989	2575875
plasma glutamate--a prognostic marker of cancer and of other immunodeficiency syndromes?	elevated plasma levels of glutamate (glu) have been reported to occur in patients with malignancies and other immunodeficiency syndromes (ids). to evaluate, whether glu is useful as prognostic indicator, the plasma concentrations were determined in patients with colorectal carcinoma (crc), with breast cancer (brc), and with hiv-infection (hiv). the results were correlated with the disease-stages, and compared with data obtained from patients with benign diseases of the same organ, as well as fro ...	1989	2575787
endocrine effects of combined somatostatin analog and bromocriptine therapy in women with advanced breast cancer.	in this pilot clinical trial conducted in 10 postmenopausal women with advanced breast cancer, we evaluated the endocrine effects and toxicity of combined somatostatin analog and dopaminergic therapy in the attempt to suppress both growth hormone (gh) and prolactin (prl) secretion. the patients' mean age was 63 years (range: 54-77) and the average number of previous treatments was 4.8 +/- 2 (sd). all patients were treated with the somatostatin analog sms 201-995 (100-200 micrograms s.c. in a.m. ...	1989	2575406
highly specific inhibition of human immunodeficiency virus type 1 by a novel 6-substituted acyclouridine derivative.	a novel 6-substituted acyclouridine derivative, 1-[(2-hydroxy-ethoxy) methyl]-6-phenylthiothymine (hept), has proved to be a potent and selective inhibitor of human immunodeficiency virus type 1 (hiv-1) in vitro. hept inhibits hiv-1 replication in various t4 cell cultures as well as peripheral blood lymphocytes and macrophages. the 50% antiviral effective concentration for hiv-1 (htlv-iiib) in mt-4 cells is 7.0 microm, while the 50% cytotoxic concentration for mock-infected mt-4 cells is 740 mic ...	1989	2575380
high levels of oral yeasts in early hiv-1 infection.	ten human immunodeficiency virus-1 (hiv-1) infected homosexual or bisexual individuals (ages 24-45) with no history of opportunistic infection were examined, by culture, for the presence of yeasts in whole saliva and on oral mucosa. all were hiv-1 antibody-positive men, non-smokers, non-denture wearers, and taking no medication. the mean salivary level of yeast was four logs higher in the hiv-1 infected group compared to a control group of normal, unmedicated, non-smoking men (ages 20-41) who de ...	1989	2575167
the long-term outcome of thyroidectomy for sporadic non-toxic goitre.	to study the long-term outcome after thyroidectomy, 113 sporadic non-toxic goitre patients who underwent thyroidectomy in our hospital in the period 1974-1983, were studied. five patients complained of recurrent goitre; a goitre was found on inspection and palpation in these five and in 15 others. there were no differences between the 20 patients with goitre and the 93 patients without goitre with regard to sex, age, duration of goitre, indication and type of thyroidectomy, postoperative thyroid ...	1989	2575018
evaluation of tsh receptor antibody by 'natural in vivo human assay' in neonates born to mothers with graves' disease.	neonatal thyrotoxicosis induced by transferred tsh receptor antibody (trab) is the ideal human in-vivo experimental system for the evaluation of trab. the clinical significance of circulating trab in graves' disease was evaluated by this 'natural in-vivo human assay'. trab activity in vitro was measured by radioreceptor assay (thyrotrophin-binding inhibitor immunoglobulin, tbii) and sensitive camp accumulation assay using frtl-5 cells (thyroid-stimulating antibody, tsab). further, the binding-st ...	1989	2575017
isolation of hiv using t-lymphocyte 'panning': methodological aspects.	a technique for isolation of hiv using selective cultures of t4 cells obtained from peripheral blood by immunochemical separation was developed and optimized. using this method infectious virus could be isolated in single isolation attempts from 89% of 35 hiv-infected patients in different stages of immunodeficiency. this isolation frequency was virtually independent of the stage of the disease, in contrast to the results obtained by the conventional isolation technique based on peripheral monon ...	1989	2574183
thyroid function in sex-linked and autosomal dwarf chickens.	thyrotropin-releasing hormone (trh) and thyrotropin (tsh) elevated plasma thyroxine (t4) and triiodothyronine (t3) concentrations in a (euthyroid) control line of chickens and in an autosomal dwarf strain. these agents were ineffective in sex-linked dwarf (sld) chickens. similarly, while somatostatin (srif) lowered plasma t4 and t3 concentrations in autosomal dwarf (adw) chickens and controls, it had no inhibitory effect in the sld strain. these results suggest that an impairment in the hypothal ...	1989	2574126
effect of fenoldopam, a dopamine d-1 receptor agonist, on pituitary, gonadal and thyroid hormone secretion.	the influence of fenoldopam, a dopamine (da) d-1 receptor agonist, on basal and gnrh/trh stimulated prl, gh, lh, tsh, testosterone and thyroid hormone secretion was studied in nine normal men. all men received 4-h infusions of either 0.9% saline or fenoldopam at an infusion rate of 0.5 microgram/kg min, 12-16 ml/h, adjusted according to weight. after 3 h of infusion, 50 micrograms gnrh and 100 micrograms trh was given i.v. blood samples were collected every 15 min from 1 h before to 1 h after th ...	1989	2574081
phase i study of low-dose zidovudine and acyclovir in asymptomatic human immunodeficiency virus seropositive individuals.	the combination of zidovudine and acyclovir has shown in vitro antiretroviral activity and led to short-term improvement in patients with symptomatic human immunodeficiency disease (hiv) disease. we performed a phase i study of zidovudine (500 mg/day) plus acyclovir (2 or 4 g/day) in asymptomatic hiv-seropositive men to investigate pharmacokinetics, safety, tolerance, and immunologic effects of the combination.	1989	2574006
hiv heterosexual transmission in hemophilia couples: lack of relation to t4 number, clinical diagnosis, or duration of hiv exposure.	six (13%) of 45 female partners of 45 hiv antibody positive [ab(+)] hemophiliacs in western pennsylvania have become hiv ab(+), a mean of 10.4 months from the time of first exposure. by comparison, the mean time from first exposure to the last/recent exposure in the hiv ab(-) female partners was 54.2 months. all six hemophiliacs who transmitted hiv to their female partners were asymptomatic, with mean t4 of 403 +/- 51/mm3 at the time of estimated seroconversion in the female; currently, their me ...	1989	2573720
comparison between constant-protein, calorie-restricted and protein-restricted, calorie-restricted diets on growth, in vitro lipogenesis and plasma growth hormone, thyroxine, triiodothyronine and somatomedin-c (sm-c) of young chickens.	1. we studied the effects of calorie-restricted, constant-protein and calorie-restricted, protein-restricted diets on growth and in vitro metabolism of male chickens from select (cobb line 500) and byproduct (cobb female line) lines of broiler chickens. 2. chickens consumed 40, 60, 80 or 100% of a prescribed formula for dietary energy (body weight in g0.70 x 16.7 kj) in the presence of set (cep) or varied dietary protein (vep). 3. chickens fed vep were heavier (p less than 0.05) at all energy in ...	1989	2573452
precursor frequency of human t4 cells responding to stimulation through the cd3 molecular complex: role of various cytokines in promoting growth and il2 production.	the frequency of human t4 cells induced to grow and produce il2 in response to the anti-cd3 mab, 64.1, was examined. t4 cells were cultured at limiting dilution and stimulated with either soluble or immobilized 64.1 in the presence of various cytokines and/or irradiated b lymphoblastoid cells as accessory cells (ac). the frequency of responding cells was assessed by examining wells microscopically for visible growth and supernatants for il2. immobilized, but not soluble, 64.1 was able to induce ...	1989	2573432
analysis of c-erbb-2 expression in breast carcinomas with clinical follow-up.	various monoclonal antibodies reactive with protooncogene products or tumor-associated antigens have been utilized to investigate breast carcinoma biology or antigen expression with potential prognostic relevance. murine monoclonal antibody ta1, generated by immunization of balb/c mice with whole c-erbb-2 (neu) transformed nih/3t3 cells, recognizes the extracellular domain of the c-erbb-2 protein and binds a mr 185,000 protein by immunoprecipitation. using avidin-biotin-peroxidase techniques and ...	1989	2573426
the natural history of human immunodeficiency virus infection in a haemophilic cohort.	112 haemophilic patients infected with hiv were followed up with clinical and laboratory assessment between 1 december 1979 and 30 november 1988. sixty-six (59%) of the patients developed hiv-related clinical symptoms and 22 (20%) developed aids. twenty (18%) of the patients developed p24 antigenaemia. amongst the 59 patients whose date of seroconversion could be estimated the calculated 8-year cumulative incidence of aids was 40% (symptoms 73%). for the whole cohort of 112 patients, the median ...	1989	2573389
intracellular ca2+ and cytotoxicity.	following injury or activation in some immune cell lines, elevation of intracellular ca2+ concentration (cai2+) is an early and major event that precedes cell death. agents shown to elevate cai2+ and to result subsequently in the death of some cells include human immunodeficiency virus (hiv) (in t4+ cells), 25-hydroxy cholesterol, tumor necrosis factor (tnf), cyclosporine, dexamethasone, alpha-interferon, and ca2+ ionophores. the effects of these agents, both on cai2+ and on cytotoxicity, are ad ...	1989	2573316
in vitro accumulation of thyroid hormones by cultured rat hepatocytes and the biliary excretion of iodothyronines in rats treated with a novel histamine h2-receptor antagonist.	the administration of sk&f 93479, a novel histamine h2 antagonist, to wistar rats has been shown to produce thyroid lesions associated with an increased clearance of plasma thyroxine (t4) and elevated plasma tsh concentrations. to determine if these changes were mediated via an increase in the hepatic clearance of thyroid hormones, the biliary clearance and hepatic accumulation of iodothyronines were measured in rats and cultured hepatocytes treated with sk&f 93479. both the in vitro and in vivo ...	1989	2573172
response to pneumococcal vaccine among asymptomatic heterosexual partners of persons with aids and intravenous drug users infected with human immunodeficiency virus.	antibody responses to pneumococcal vaccine were studied in asymptomatic heterosexual partners of persons with aids and intravenous drug users seropositive for human immunodeficiency virus (hiv). serum antibodies to 12 pneumococcal capsular antigens were measured by radioimmunoassay. eleven intravenous drug users seropositive for hiv, 13 seronegative intravenous drug users, and 10 each seropositive and seronegative sexual partners received 23-valent pneumococcal vaccine. additional unvaccinated m ...	1989	2572650
[study of ige and t lymphocyte subsets in patients with serious bronchial asthma].	in the present study, 30 patients with serious asthma were selected for the investigation of ige and t lymphocyte subsets in pbl. by the indirect mcab immuno-spa assay, it was observed that the percentages of okt3 and okt8 but the percentage of okt4 subset and t4/t8 ratio were significantly higher than those of normal persons (p less than 0.01). the total ige in serum of patients (performed by elisa) was also much higher than that of normal persons (p less than 0.05). in addition, there was a cl ...	1989	2572332
effect of rate of injection on the neuromuscular block produced by vecuronium.	a subparalytic dose (0.015 mg/kg) of vecuronium bromide was administered to matched pairs of patients undergoing routine dental surgery under enflurane-nitrous oxide/oxygen anesthesia either as a rapid bolus injection or as a slow infusion over 5 min. it was demonstrated that bolus injection produced peak plasma levels of drug considerably greater than those following slow infusion. time to maximum block was more rapid following bolus (368 +/- 84 [sd] sec) than by infusion (615 +/- 88 sec), but ...	1989	2572184
glucoregulatory function of thyroid hormones: evidence for an insulin-independent effect.	the effect of hyperglycemia on glucose kinetics was investigated in normal and t4-treated miniature pigs. 1. t4-treatment increased basal plasma glucose (g) (+ 20%) and glucose turnover (+36%). 2. glucose infusion (2 mg/kg x min) in controls increased insulin and glucose utilization (rd), but decreased glucagon and hepatic glucose production (ra). after t4-treatment glucose increased insulin, decreased glucagon and ra but only a slight effect on g and rd were observed. 3. infusing glucose + soma ...	1989	2570710
detection of hiv-1 dna in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. rapid communication.	the presence of hiv-1 proviral dna was determined in cd4 cells, cd8 cells and macrophages/monocytes obtained from peripheral blood of 8 hiv-1 infected persons. using the polymerase chain reaction (pcr) we were able to detect proviral dna in the extracts of only 10(2)-10(3) cd4 (t4) cells. in contrast, 10(5) cd8 cells did not contain detectable amounts of proviral dna. surprisingly, in four of our eight patients studied no hiv-1 dna was found in macrophages. in peripheral blood, every hundred t4 ...	1989	2570562
mass and in situ molar activity of tyrosine hydroxylase in the median eminence. effect of thyroidectomy and thyroid hormone replacement.	the effects of thyroidectomy and thyroid hormone replacement on the mass and in situ molar activity of tyrosine hydroxylase (th) in the median eminence (me) and superior cervical ganglia (scg) of male rats were investigated. the tissue specificity of these effects were evaluated by comparing the me with the superior cervical ganglion (scg). all animals were thyroparathyroidectomized (tx) or sham tx. tx rats were treated daily for 3 weeks with 0.15 m nacl (solvent vehicle) or l-thyroxine (t4). tw ...	1989	2570369
a role for cd4+ but not cd8+ t cells in immunity to schistosoma mansoni induced by 20 krad-irradiated and ro 11-3128-terminated infections.	the role of cd4+ (l3/t4+) and cd8+ (lyt-2+) t cells in immunity to schistosoma mansoni induced by 20 krad-irradiated and ro 11-terminated infections in mice was investigated directly by in vivo depletion of these subsets with cytotoxic rat monoclonal antibodies (mab). effective physical depletion was demonstrated by flow cytometric analysis and immunohistochemical staining. functional depletion of helper activity following anti-cd4 treatment was indicated by an abrogation of concanavalin a(con a ...	1989	2570035
preparation of misacylated aminoacyl-trna(phe)'s useful as probes of the ribosomal acceptor site.	several pyroglutamylaminoacyl-trna's were prepared by t4 rna ligase mediated condensation of synthetic pyroglutamylaminoacyl-pcpa's with trna's from which the last two nucleotides at the 3'-end had been removed. the derived pyroglutamylaminoacyl-trna's were incubated in the presence of calf liver pyroglutamate aminopeptidase, which effected their conversion to free aminoacyl-trna's. the lack of contaminating esterase activities in the pyroglutamate aminopeptidase was verified by direct assay for ...	1989	2569893
studies on lymphocyte subpopulations and nk cell activities in epileptic patients.	the subpopulations of t lymphocytes, b lymphocytes and macrophages of the peripheral blood in 58 cases of epilepsia were studied with mcab by ifa method. the activity of natural killer (nk) cells of peripheric blood monocyte was investigated by 51cr-releasing method. 22 healthy donors of about the same age served as controls. the results showed that the percentage of t3 and t4 lymphocytes in epileptic patients was decreased, and the ratio of t4/t8 was markedly reduced as compared to control grou ...	1989	2569541
spread of analgesia and ventilatory response to carbon dioxide following epidural somatostatin.	the effects of somatostatin, injected into the epidural space, on analgesia and control of ventilation were studied in 25 patients aged 41 +/- 9 yrs (mean +/- sd). the patients were allocated to three groups to receive: group i--1 mg of somatostatin in 2 ml saline (n = 13); group ii--1 mg of somatostatin in 10 ml saline (n = 6); and group iii--somatostatin in a loading dose of 250 micrograms followed by an infusion of 125 micrograms h-1 (n = 6). segmental cutaneous analgesia, assessed by pinpric ...	1989	2569394
levels of t4, t3 and cortisol in the blood serum of the european bison (bison bonasus) in the winter period.	1. in the blood of 56 european bison, the levels of t4, t3, and the ratio of t3:t4 and cortisol were studied. 2. between december and march, the t4 level changed from about 53 to 83 ng/ml of blood serum but in april it increased to 90 ng/ml. 3. the level of t3 in this period was 0.817-1.475 ng/ml and in april it was 2.40-3.40 ng/ml. 4. the ratio of t3:t4 was 0.015-0.024 and in april it increased to 0.027-0.052. 5. the level of cortisol changed from 1.00 to 6.70 ng/ml and in april it was from 1.0 ...	1989	2569374
atracurium and vecuronium interaction with gentamicin and tobramycin.	drug interactions between the aminoglycosides (tobramycin and gentamicin) and atracurium and vecuronium were studied prospectively in 44 patients. twenty-two patients had therapeutic serum levels of tobramycin or gentamicin and 22 served as controls. onset time, clinical duration, and time to spontaneous recovery of t4/t1 ratio of 0.70 after atracurium or vecuronium injection were measured. no statistically significant differences were found in onset time, but clinical duration and time to recov ...	1989	2569365
okt4/okt8 ratio in asymptomatic heroin addicts.	the okt4 (helper) and okt8 (suppressor) lymphocytic subpopulations were enumerated in a sample of 60 asymptomatic drug addicts and in 17 controls. no significant differences in the ratio could be found that could not be explained by the action of hiv. it can be concluded that heroin itself was not responsible for any alteration in the t4/t8 ratio in the population considered.	1989	2569333
advanced immunodeficiency as a risk factor for heterosexual transmission of hiv.	to define the rate of hiv infection and associated risk factors among heterosexual partners of hiv-infected individuals, 80 partners and 77 index cases were examined for clinical, behavioural and virological variables. the study population included 61 male and 16 female index cases, of whom 40 were european and 37 african. multiple sexual partners in central africa was the risk factor for hiv infection in 83%. the overall infection rate among the contact cases was 45%, 53% among female partners ...	1989	2568844
regulatory t-cell function in primary humoral immunodeficiency states.	the function of b cells and highly purified t-cell subpopulations was studied in patients with various primary humoral immunodeficiencies. in the presence of normal t4+ cells b cells of the patients with x-linked agammaglobulinemia (xla) were not capable either for igm or igg synthesis, whereas b cells of the patient with hyper-igm syndrome and three of eight patients with common variable immunodeficiency (cvi) could produce normal amounts of igm, but not igg. all patients with xla and the patie ...	1989	2568491
in vivo depletion of bot4 (cd4) and of non-t4/t8 lymphocyte subsets in cattle with monoclonal antibodies.	the effect on certain immune responses of depleting two distinct lymphocyte subpopulations in vivo by inoculating calves with monoclonal antibodies (mab) was examined. an mab directed against the bot4 antigen (the bovine homologue of cd4) effectively removed the bot4+ lymphocytes from peripheral blood mononuclear cells (pbm). compared to controls, treated calves showed a reduced antibody response to human o red blood cells and to ovalbumin. pbm prepared from bot4-depleted animals also had a sign ...	1989	2567247
effective method for prediction of transient hypothyroidism in neonates born to mothers with chronic thyroiditis.	an effective method of prediction of neonatal transient hypothyroidism was examined in 105 neonates (including a pair of twins) born to mothers with chronic thyroiditis (92 mothers with goitrous hashimoto's disease and 12 with primary atrophic hypothyroidism). antithyroid microsomal antibody was measured by a hemagglutination technique (mcha), and antithyroid-stimulating hormone (tsh) receptor antibody by both radioreceptor assay (tbii) and biologic thyroid-stimulation blocking assay (tsbab). fo ...	1989	2567172
predictive markers for the acquired immunodeficiency syndrome (aids) in hemophiliacs: persistence of p24 antigen and low t4 cell count.	to investigate the predictive value of assays for human immunodeficiency virus (hiv) p24 antigen, p24 antibody, and gp120 antibody compared with t4 cell counts.	1989	2567142
hsv hepatitis in the mouse: a light and electron microscopic study with immunohistology and in situ hybridization.	in order to characterize better the morphology and immune response in acute necrotizing hsv infection, murine hsv hepatitis was examined. balb/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (pfu) of hsv-1 (lenette) and hsv-2 (d316). in both groups half the animals were pretreated with silica particles to block macrophage function. up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron ...	1989	2567083
neopterin: a predictive marker of acquired immune deficiency syndrome in human immunodeficiency virus infection.	in 79 homosexual men infected with human immunodeficiency virus (hiv), urinary neopterin was significantly higher as compared with 70 hiv-seronegative men in the same cohort (p less than 0.0001). this highly significant association was found both for naturally occurring oxidized (native) neopterin and for total neopterin (native plus chemically oxidized forms). in prospective follow-up for 18 months, the odds that the acquired immune deficiency syndrome (aids) would develop were elevated 25-fold ...	1989	2566673
seasonal levels of cortisol, triiodothyronine and thyroxine in male axis deer.	1. seasonal plasma levels of thyroxine (t4), triiodothyronine (t3) and cortisol were investigated between november and june in seven penned male axis deer. 2. no distinct seasonal variation of cortisol has been detected. the levels oscillated between 1 and 5 micrograms/dl. 3. the stress of immobilization and sampling had little effect on cortisol levels. concentrations remained mostly stable in three consecutive samples taken 10 min apart. 4. t3 concentrations were stable between november and ma ...	1989	2566417
expression of hiv antigens at the surface of infected t4 cells: immunoelectron microscopic evidence of an immunogenic phase prior to the viral release.	hiv antigens were detected by immunoelectron microscopy at the surface of human and simian t4 lymphocytes that had been infected in vitro. hiv antigens were detected at the surface of cells exhibiting viral particles but also at the surface of cells before the release of virions. the latter cells may be considered immunogenic since they are capable of triggering specific immune responses without the cytopathic effects due to viral release.	1989	2566320
t4+ cell production of interferon gamma and the clinical spectrum of patients at risk for and with acquired immunodeficiency syndrome.		1989	2566301
graves' hyperthyroidism following primary hypothyroidism: sequential changes in various activities of thyrotropin receptor antibodies.	a 40-year-old male who developed graves' hyperthyroidism following primary hypothyroidism is reported. he presented with clinical signs of hypothyroidism and concomitant myasthenia gravis. the thyroid was not palpable. he was treated with t4, pyridostigmine and prednisolone. one year later he developed hyperthyroidism and goitre. his initial serum igg had no intrinsic thyroid stimulating activity, but showed almost complete inhibition of tsh-stimulated camp generation (99.4%, normal less than 38 ...	1989	2566247
specific interaction of aurintricarboxylic acid with the human immunodeficiency virus/cd4 cell receptor.	the triphenylmethane derivative aurintricarboxylic acid (ata), but not aurin, selectively prevented the binding of okt4a/leu-3a monoclonal antibody (mab) and, to a lesser extent, okt4 mab to the cd4 cell receptor for human immunodeficiency virus type 1 (hiv-1). the effect was seen within 1 min at an ata concentration of 10 microm in various t4+ cells (mt-4, u-937, peripheral blood lymphocytes, and monocytes). it was dose-dependent and reversible. ata prevented the attachment of radiolabeled hiv- ...	1989	2566170
prognostic factors and staging classification of patients with epidemic kaposi's sarcoma.	two hundred twelve patients with acquired immune deficiency syndrome (aids)-related kaposi's sarcoma (ks) were followed prospectively. univariate and multivariate analyses were performed to determine significant predictors of survival and development of opportunistic infection (oi) from the time of diagnosis of ks. clinical variables analyzed were age at onset, presence of systemic symptoms, prior or coexistent oi, development of oi greater than 3 months following ks diagnosis, and extent of dis ...	1989	2565954
lymphocyte subpopulation with low membrane potential in the blood of cyclosporin- and prednisone-treated patients: in vivo selectivity for t4 subset.	previous work on the mode of action of csa revealed that this drug shifts the membrane potential of human periferal blood lymphocytes in vitro. recently we have analyzed lymphocytes of cyclosporin-treated transplant patients. membrane potential analysis with the dioc6(3) fluorescence dye indicates that all the studied patients have a subpopulation of lymphocytes with low membrane potential and that this population is made up predominently of okt4+ cells. however there is no correlation between t ...	1989	2565726
immunosuppression in men with bowenoid papulosis.	the immune status of four men with bowenoid papulosis was evaluated. each case had been refractory to multiple methods of treatment. three of the men had other infections and demonstrated a depletion of t4-helper cells. two of these patients were anergic on skin testing, and the third showed weak reactivity. the fourth patient, who had no evidence of additional infections, had a normal t4 value and t4/t8 ratio, but was anergic on skin testing. all the men were serologically negative for human im ...	1989	2565705
progression to aids in patients with lymphadenopathy or aids-related complex: reappraisal of risk and predictive factors.	in 1985, we reported that acquired immunodeficiency syndrome (aids) developed in 14 of 81 (17%) men with generalized lymphadenopathy followed prospectively for an average of 13 months. the presence of oral thrush or constitutional symptoms, or both, or severely impaired t4+ cell responses to specific antigen (interferon-gamma production) accurately identified patients at immediate risk for aids. the purpose of the current report is to describe the progress of these 81 patients during the three a ...	1989	2565690