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xanthomonas maltophilia bacteremia: an analysis of 32 cases.thirty-two cases of xanthomonas maltophilia bacteremia have been identified over the last two years at the veterans general hospital, taipei. among them, 27 cases (84%) were due to hospital-acquired infections, and 14 cases (44%) were polymicrobial bacteremia. one case was confirmed as prosthetic valve endocarditis and one case was complicated by recurrent attacks of ecthyma gangrenosum. most cases had severe debilitating conditions. twelve cases (38%) had a malignancy, 19 cases (59%) were resid ...19921363639
bacteraemia caused by non-aeruginosa pseudomonas species in a cancer centre.fifty-one episodes of bacteraemia due to pseudomonas species other than pseudomonas aeruginosa occurring between 1980 and 1990 in a belgian cancer centre were reviewed. this corresponded to an incidence of 0.62/1000 admissions, or 1.5% of all bacteraemic episodes. twenty-nine episodes, each with several positive blood culture sets were considered clinically significant, including six patients belonging to a well-documented outbreak of pseudobacteraemia with xanthomonas maltophilia and associated ...19921363110
microbial contamination of brushes used for preoperative shaving.microbial contamination of brushes used for preoperative shaving was investigated. of the 24 brushed examined, 18 were contaminated with 10(6)-10(9) colony forming units (cfu) per brush. non-fermentative gram-negative bacilli such as pseudomonas aeruginosa and xanthomonas maltophilia, and yeast-like fungi such as candida parapsilosis were the primary contaminants. the mean bacterial count on the skin after the use of contaminated brushes (having a mean bacterial count 2.2 x 10(8) cfu) in 14 subj ...19921353085
xanthomonas maltophilia endophthalmitis after implantation of sustained-release ganciclovir. 19921334377
in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy quinolone.am-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ...19921332587
in vitro activity of cp-74667 compared with four other fluoroquinolones.cp-74667 is a new, novel c-7 diazabicyclo-fluoroquinolone. its spectrum of activity includes the enterobacteriaceae, most nonenteric gram-negative bacilli, and gram-positive cocci. particularly high activity was demonstrated against xanthomonas maltophilia (mic50, 1 microgram/ml), staphylococcus spp. (mic50s, 0.06-0.12 micrograms/ml) and enterococci (mic50s, 0.5-4 micrograms/ml). several staphylococci resistant to ciprofloxacin had potentially susceptible range mics for cp-74667, for example, le ...19921330419
in vitro evaluation of e-4695, a new fluoro-naphthyridine.compound e-4695, a c-7 azetidinyl fluoro-naphthyridine, was compared to six structurally related quinolones and was generally two- to four-fold more active than ciprofloxacin. e-4695 was particularly active against staphylococcus aureus (mic90 0.06 mg/l), staphylococcus haemolyticus (mic90 0.5 mg/l), klebsiella spp. (mic90 less than or equal to 0.015 mg/l), serratia marcescens (mic90 0.06 mg/l), acinetobacter spp. (mic90 less than or equal to 0.015 mg/l), pseudomonas aeruginosa (mic90 0.5 mg/l), ...19921327787
in vitro activity of sparfloxacin compared with those of five other quinolones.the in vitro activity of sparfloxacin, a new difluorinated quinolone, was evaluated against 857 gram-positive and gram-negative clinical isolates and compared with those of ciprofloxacin, norfloxacin, ofloxacin, fleroxacin, and lomefloxacin. the mic of sparfloxacin for 90% of the members of the family enterobacteriaceae tested was 0.5 microgram/ml (range, 0.06 to 4.0 micrograms/ml); only for members of the genera serratia, citrobacter, and providencia were mics above 1 microgram/ml. some 90% of ...19921320362
in vitro activity of sparfloxacin (ci-978), a new broad-spectrum fluoroquinolone.the in vitro activity of sparfloxacin (ci-978, at-4140), a new fluoroquinolone, was compared with ciprofloxacin, norfloxacin and other commonly used antimicrobial agents against 650 strains of enterobacteriaceae, 237 isolates of other gram-negative bacilli and 318 strains of gram-positive cocci. the mics of sparfloxacin against 90% of the members of enterobacteriaceae were between 0.12 and 0.5 microgram/ml. all the 48 isolates of notoriously drug-resistant serratia marcescens were inhibited by l ...19921317282
[studies on human chorionic gonadotropin (hcg)-binding protein from pseudomonas maltophilia by ligand blotting assay].a ligand blotting technique was developed to study the hcg-binding protein from pseudomonas maltophilia after size separation by sds-polyacrylamide gel electrophoresis under nonreducing conditions. the separated proteins were transferred to a nitrocellulose sheet, which was subsequently incubated with [125i]iodo hcg, and subjected to determination for radioactivity in gamma-counter. a radioactivity peak equivalent to an m(r) 70000 appeared, which was not observed when the hormone incubation was ...19921304539
antibacterial in vitro-activity of meropenem against 200 clinical isolates in comparison to 11 selected antibiotics.the antimicrobial activity of meropenem, a new parenteral carbapenem, was tested in vitro by an agar dilution method against 200 clinical isolates (gram-negative/positive aerobes and anaerobes). meropenem was compared with imipenem, ceftazidime, cefotaxime, piperacillin, ciprofloxacin, gentamicin; and metronidazole, cefoxitin, chloramphenicol, clindamycin, vancomycin when appropriate. meropenem and imipenem exhibited an extended spectrum of activity with low minimal inhibitory concentrations (mi ...19921303691
comparison of the autoscan-w/a and vitek automicrobic systems for identification and susceptibility testing of bacteria.the identification and susceptibility testing of bacteria by the autoscan-w/a rapid panels and vitek automicrobic systems were compared. a total of 291 clinical isolates, including 207 members of the enterobacteriaceae, 41 nonfermentative gram-negative bacilli, 31 staphylococcus aureus strains and 12 enterococcus faecalis strains, were tested. autoscan-w/a and vitek correctly identified 93% and 94% respectively of the enterobacteriaceae, the identification in an additional 2% and 5% of strains r ...19921295766
activity of meropenem against imipenem-resistant bacteria and selection in vitro of carbapenem-resistant enterobacteriaceae.the activity of meropenem against 106 imipenem-resistant (mic > or = 8 mg/l) clinical isolates, and the frequency of resistance to meropenem and imipenem among 24 enterobacteriaceae was determined. both agents selected colonies on agar but 20-80% were susceptible after one subculture and 72% of the mutants reverted to susceptibility 1 to 6 months after selection. all isolates and stable mutants were inhibited by > 1 mg/l meropenem, although the mic of imipenem was 4-16 mg/l. three of six xanthom ...19921291320
[comparative studies on lh/cg receptor from rat testes and hcg-binding protein from pseudomonas maltophilia by antibodies against the hcg-binding protein].an indirect immunoprecipitation experiment was developed to characterize the antigenic determinants of the hcg-binding protein and the rat testicular lh/cg receptor. the hcg-binding protein preincubated with 125i iodo hcg was immunologically precipitated by the antibodies against the hcg-binding protein in the presence of goat anti-rabbit igg. similarly, the lh/cg receptor solubilized with 1% triton x-100 from the rat testes was also immunoprecipitated by the antibodies. furthermore, the antibod ...19921284344
intracellular peptide hydrolysis by pseudomonas putida and pseudomonas maltophilia.amino acids liberated by peptidase hydrolysis of di- and oligopeptides by pseudomonas putida were measured by trinitrobenzenesulphonate assay and high voltage electrophoresis or paper chromatography followed by ninhydrin spray. intact bacteria or periplasmic contents released by lysozyme treatment did not hydrolyse peptides. subcellular fractionation showed that glycylmethionine peptidase activity was cytoplasmic. this enzyme had a km of 2 mm, and was stimulated fivefold by i mm-co2+. crude pept ...19761255132
peptide utilization by pseudomonas putida and pseudomonas maltophilia.pseudomonas putida assimilates peptides and hydrolyses them with intracellular peptidases. amino acid auxotrophs (his, trp, thr or met) grew on a variety of di- and tripeptides up to twice as slowly as with free amino acids. pseudomonas putida has separate uptake systems for both dipeptides and oligopeptides (three or more residues). although the dipeptide system transported a variety of structurally diverse dipeptides it did not transport peptides having either unprotonatable n-terminal amino g ...19761255131
meningitis caused by pseudomonas maltophilia.a case of meningitis caused by pseudomonas maltophilia is described, which was unusual in that it appeared to lack the predisposing factors commonly associated with this organism. attention is drawn to the difficulties which may be encountered in the identification of ps. maltophilia.19751165296
regulation of extracellular protease secretion in pseudomonas maltophilia.cells grown in minimal medium and harvested in late exponential phase secreted extracellular protease linearly when suspended at high density in fresh medium. if the cells were suspended with 0.2% (wt/vol) yeast extract and no additional carbon source, the rate of exoenzyme production was increased several-fold. when pyruvate, l-malate, succinate, or alpha-ketoglutarate was added, repression of exoenzyme secretion was observed. the most effective repressor was alpha-ketoglutarate. these compound ...19751158851
in vitro susceptibility of pseudomanas cepacia and pseudomonas maltophilia to trimethoprim and trimethoprim-sulfamethoxazole.our earlier studies had shown that the two pseudomonads, pseudomonas cepacia and pseudomonas maltophilia, were organisms that were highly resistant to most antibiotics. the present study was undertaken to determine the susceptibility of these bacteria to trimethoprim and the trimethoprim-sulfamethoxazole combination which has been used with apparent success in treating infections caused by these pseudomonads. all 51 strains of p. cepacia were inhibited by 2 mug or less of trimethoprim per ml, wh ...19751155926
infection with pseudomonas maltophilia hospital outbreak due to contaminated disinfectant.between august, 1973, and may, 1974, pseudomonas maltophilia was isolated from 63 patients in the king edward memorial hospital for women, subiaco, western australia. primary cultures were obtained mainly from specimens of urine from adults, and from intraarterial catheter tips and umbilical swabs from neonates. in most cases the organism appeared to be a contaminant, but in seven it was responsible for infection. the source of the organism was deionized water used for diluting savlon concentrat ...19761004317
[characterization of telluric bacteria attributable to pseudomonas maltophilia].the taxonomic position of some telluric non-fermenting gram-negative bacteria collected into a phenon representing about a quarter of the soil isolates by an earlier numerical analysis is specified. the shapiro--chargaff coefficient determined by thermal-transition curves of the dna and their hybridization by nitrocellulose filters method show a close affinity with pseudomonas maltophilia.1977907912
[pseudomonas maltophilia in cerebrospinal fluid samples. isolation, identification and antibiograms of 4 strains]. 1977897286
pseudomonas maltophilia bacteremia associated with a prolapsed mitral valve.a 53-year-old man had had recurrent episodes of transient visual loss, malaise and a heart murmur. blood cultures repeatedly grew pseudomonas maltophilia, a frequent opportunistic pathogen, and echocardiogram documented mitral-valve prolapse. the risk of bacterial endocarditis is stressed.1977879107
presence of 3-o-methyl-l-xylose in the lipopolysaccharide of pseudomonas maltophilia n.c.t.c. 10257.3-o-methyl-l-xylose was isolated from whole cells of pseudomonas maltophilia n.c.t.c. 10257. the sugar is a component of lipopolysaccharide from which a polysaccharide also containing l-rhamnose and l-xylose was released by mild acid hydrolysis. 3-o-methyl-l-xylose was absent from five other strains of ps. maltophilia and one strain of pseudomonas geniculata.1977869917
pseudomonas maltophilia infection in an abattoir worker.a case of infection with pseudomonas maltophilia is described. the clinical record and bacteriological findings are reported, and the possible aetiology is discussed.1977865359
competitive effects of intestinal microflora on vibrio cholerae in gnotobiotic mice.the coexistence of vibrio cholerae and several intestinal bacteria was determined in gnotobiotic mice. the bacteria tested included a bacteroides sp, clostridium difficile, clostridium perfringens type a, 2 separate isolates of escherichia coli, 2 different lactobacilli, 2 separate isolates of proteus mirabilis, pseudomonas maltophilia, and streptococcus faecalis. each species of bacteria became established and was recovered repeatedly from the stools during the studies. no single strain or spec ...1975807775
bacterial variants: etiologic agent in crohn's disease?bacterial variants of pseudomonas maltophilia and pseudomonas-like bacteria were recovered from tissues removed during the surgical treatment of three successive patients with crohn's disease and from one patient with clinical and pathological features of both crohn's disease and chronic ulcerative colitis. bacterial variants were not cultured from colonic specimens of one patient with classical features of chronic ulcerative colitis and two patients with adenocarcinoma of the colon. the finding ...1976780185
pseudomonas maltophilia causing heroin-associated infective endocarditis.the association of pseudomonas maltophilia endocarditis in three patients with recent history of intravenous drug abuse is reported. all three patients had abnormal heart valves (two prosthetic and one rheumatic). a prominent characteristic of this uncommon pathogen is its in vitro resistance to the commonly used antimicrobials. cure was achieved in all three cases. in two cases, synergistic antibiotic combinations were used. in one case, plasmid-mediated resistance to amikacin sulfate (amikan, ...1978718316
aminoglycoside 6'-n acetyltransferase production by an isolate of pseudomonas maltophilia. 1978690050
pseudomonas maltophilia: identification of the hydrocarbons, glycerides, and glycolipoproteins of cellular lipids.the nature of quantity of the free lipids of pseudomonas maltophilia have been examined. lipid components identified as phosphatidyl ethanolamine, phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidylamino acid derivatives and phosphatidic acid accounted for about one-half of the total lipids and are generally typical of gram-negative bacteria. the remaining lipids were identified as a complex family of aliphatic hydrocarbon isomers (c22--c32) and a mixture of glycolipoproteins. these com ...1978657006
[clinical and bacteriological study on pseudomonas maltophilia (author's transl)]. 1978642210
pseudomonas maltophilia pseudosepticemia.during a 17 month period, 25 hospitalized adult patients had blood cultures reported as positive for pseudomonas maltophila. review of the hospital records suggested that these were contaminants and that blood for coagulation studies and for cultures that were subsequently positive had been drawn simultaneously. the source of contamination appeared to be black-top evacuated collection tubes used for coagulation studies in adults. cultures of the liquid anticoagulant tubes yielded a pure growth o ...1978637055
[study of 2 cases of pseudomonas maltophilia meningitis observed in senegal]. 1977589735
susceptibility of pseudomonas maltophilia to antimicrobial agents, singly and in combination.pseudomonas maltophilia is resistant to most of the commonly used antimicrobial agents including those active against pseudomonas aeruginosa. the susceptibility of 14 clinical isolates of p. maltophilia to 18 antimicrobial agents was determined by broth dilution testing. all organisms were susceptible to trimethoprim-sulfamethoxazole (tmp-smz), minocycline, and ly127935. a total of 87 and 79% of the organisms were susceptible in vitro to colistin and chloramphenicol, respectively. with the excep ...1979533264
the sensitivity of gentamicin-resistant gram-negative bacilli to cefotaxime, other cephalosporins and aminoglycosides.the sensitivities of 80 gentamicin-resistant gram-negative bacilli to cefotaxime, cefuroxime, cefoxitin, cefamandole, cefazolin, tobramycin, netilmicin and amikacin were determined. amikacin was the most active amino-glycoside. however, the percentage sensitivity to cefotaxime of most of the species was higher than, or equal to any of the other antibiotics tested. cefotaxime was particulary active against providencia spp., serratia spp., klebsiella spp., and pseudomonas maltophilia, being 16 to ...1979478651
distribution in clinical material and identification of pseudomonas maltophilia.during the 10-year period ending march 1976, 128 (8.5%) of 1506 strains of gram-negative non-fermentative bacteria submitted to the national collection of type cultures for computer-assisted identification have been strains of pseudomonas maltophilia. these figures suggest that ps. maltophilia is both a relatively common species in clinical material in the united kingdom and also that many laboratories experience difficulty in identifying this species. we report the sources from which our strain ...1979429581
lipopolysaccharides from pseudomonas maltophilia: structural studies of the side-chain polysaccharide from strain n.c.t.c. 10257.a lipopolysaccharide has been isolated from pseudomonas maltophilia n.c.t.c. 10257. monosaccharide components identified were l-rhamnose, 3-o-methyl-l-xylose, l-xylose, d-glucose, d-mannose, d-galacturonic acid, 2-amino-2-deoxy-galactose, 2-amino-2-deoxyglucose, and a 3-deoxy-2-octulosonic acid. heptose was absent. in this and other respects, the lipopolysaccharide resembles the corresponding products from xanthomonas species. mild hydrolysis of the lipopolysaccharide with acid, followed by chro ...1979427832
physiological responses of bacteria to cytochalasin a: effects on growth, transport, and enzyme induction.cytochalasin a at 5 to 25 microgram/ml (1.0 x 10(-5) to 5.2 x 10(-5) m) inhibited the growth of three gram-positive bacteria, arthrobacter sialophilus, staphyloccus aureus, and bacillus amyloliquifaciens, but had little or no effect on the growth of three gram-negative bacteria, excherichia coli, pseudomonas maltophilia, and aeromonas proteolytica. a. sialophilus and s. aureus recovered spontaneously from cytochalasin a-mediated growth inhibition after a considerable lag period, which was depend ...1979422516
primary pseudomonas maltophilia infection of the lung.to our knowledge, this is the first reported case of primary pseudomonas maltophilia pneumonia. presenting symptoms were fever and chills of two days' duration and a density in the right upper lobe. sputum culture showed normal flora, and multiple blood cultures were negative. antibiotic therapy initially with penicillin and then with carbenicillin was unsuccessful. selective bronchial aspiration yielded pure cultures of p maltophilia. when an appropriate antibiotic, chloramphenicol, was given, ...1979419020
nonfermentative bacilli: evaluation of three systems for identification.three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ...1979389945
gentamicin resistance in christchurch hospitals.prior to june 1976, the isolation of gentamicin resistant organisms was an infrequent occurrence in north canterbury hospital board institutions. during july 1976, 20 different gentamicin resistant organisms were isolated from patients in christchurch hospital. gentamicin resistant organisms hav e been continually isolated from an increasingly wide area since then. the organisms involved are: providence species; pseudomonas aeruginosa; klebsiella species; e coli; staphyloccus aureus; proteus mir ...1979381994
specific gonadotropin binding to pseudomonas maltophilia.binding of 125i-labeled human chorionic gonadotropin to pseudomonas maltophilia is dependent on time, temperature, and ph and the binding to this procaryotic species is hormone-specific and saturable. the equilibrium dissociation constant is 2.3 x 10(-9) m. there are no cooperative interactions between binding sites (hill coefficient, 1.05). the number of sites is estimaated as 240 fmol/100 mug of protein. nacl and kcl, at concentrations from 1 to 10 mm, have no effect on binding. divalent catio ...1977265583
metabolism of glycosides by pseudomonas maltophilia.a study of the abilities of 23 strains of pseudomonas maltophilia to hydrolyze synthetically-prepared and naturally-occurring glycosides is presented. direct detection of liberated aglycones was used to determine hydrolysis of the five most-commonly-used glycosides (amygdalin, arbutin, esculin, onpg (o-nitrophenyl-beta-d-galactoside), and salicin). the capabilities of the strain for acid production from 17 glycoside substrates were also determined using a medium designed to minimize the producti ...1978250499
purification and properties of a serine protease from pseudomonas matophilia.the extracellular protease of pseudomonas maltophilia was partially purified by ammonium sulfate precipitation and chromatography on sephadex g-75 and bio-rex 70. gel electrophoresis revealed minor impurities. the enzyme exhibited the following properties: (i) molecular weight, 35,000; (ii) a see article; 10.8; (iii) isoelectric point, 9.3; (iv) ph optimum, 10.0; (v)s20, w equal 3.47. the enzyme was rapidly inactivated by ethylenediaminetetracetate, but activity could be partially restored with ...1975234950
[phosphohydrolase activity of pseudomonas maltophilia].the activity of phosphohydrolase (acid, alkaline, pyrophosphatase) was studied in two strains of pseudomonas maltophilia: vcm-b-591 and sse-b-715. the activity of these enzyme systems in these strains was found to be much higher than in other pseudomonas species, viz. ps. aeruginosa vcm-b-889, ps. fluorescens vcm-b-553, ps. geniculata sse-b-338, and in e. coli b. the phosphohydrolase activity of ps. maltophilia varied depending on the growth phase of the culture. the highest activity of acid pho ...1979231188
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
search by immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii in crohn's disease.crohn's-disease tissue was tested by indirect immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii. no reactions were obtained with anti-serum to p. maltophilia and m. kansasii, and a granular fluorescence seen with rotavirus antibody was probably non-specific.197880631
production of an extracellular ribonuclease by pseudomonas maltophilia.as part of a screening program for pseudomonad enzymes having an industrial interest, we selected ribonuclease (rnase) producing strains. of the 150 pseudomonads screened, 6 were found to produce an extracellular rnase activity when grown on solid medium. in broth culture, the rnase activity from these six species remained bound to the cells unless gelatin was added to the medium. gelatin was essential for the release of rnase in the broth culture, but the ph of the medium, addition of potential ...197936976
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