Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immune response to trichinella epitopes: the antiphosphorylcholine plaque-forming cell response during the biological cycle. | phosphorylcholine (pc), an immunodominant component of the cell wall of certain bacteria, fungi and nematodes, is known to induce low anti-pc antibody levels during natural infection by trichinella spiralis. this article reports a study in which spleen cells from bcf1 mice infected with trichinella sp. larvae were found to produce large numbers of direct haemolytic plaques in response to pc conjugated to sheep red blood cells (srbc) after muscle-encysted larvae had been killed by treatment with ... | 1987 | 2441342 |
| simultaneous isolation of preparative amounts of rna and dna from trichinella spiralis by cesium trifluoroacetate isopycnic centrifugation. | a rapid method for simultaneously banding preparative amounts of rna and dna from trichinella spiralis muscle larvae by isopycnic centrifugation in cesium trifluoroacetate (cstfa) is described. larvae were homogenized in guanidinium isothiocyanate and the dna, rna, glycogen, and denatured protein components were isopycnically separated without prior purification. this procedure resulted in the isolation of nucleic acids suitable for molecular biological application. agarose gel electrophoresis o ... | 1987 | 2440349 |
| lymphokine production by t cells generated during infection with trichinella spiralis. | lymphokine production by mesenteric lymph node cells (mlnc) taken from mice during infection with the intestinal nematode parasite trichinella spiralis was investigated. upon stimulation in vitro with a protective crude antigen preparation of the infective l1 larvae, mlnc proliferated in a specific manner, and were observed to release the t-cell lymphokines il-2, il-3 and interferon. il-2 and il-3 release by mlnc was greatest when taken during the early intestinal phases of infection. interferon ... | 1987 | 2437059 |
| trichinella spiralis: immunochemical characterization of antigens in experimental infections. | 1987 | 2436937 | |
| conservation of diagnostic antigen epitopes among biologically diverse isolates of trichinella spiralis. | crude and immunoaffinity-purified excretory-secretory antigens derived from a domestic pig isolate of trichinella spiralis were used in an enzyme-linked immunosorbent assay to test serum from mice infected with 25 different pig and wild animal isolates of t. spiralis sspp. all of the sera were found positive by elisa using either of the antigen preparations, indicating all isolates shared certain antigen epitopes. excretory-secretory antigens were prepared from 3 distinct isolates of t. spiralis ... | 1986 | 2434638 |
| immunization of mice with surface antigens from the muscle larvae of trichinella spiralis. | surface antigens of muscle larvae of trichinella spiralis were stripped from the cuticle of live worms by the cationic detergent cetyltrimethylammonium bromide (ctab). such antigen preparations were shown to contain surface antigens of approximate molecular weights 100,000, 90,000, 69,000, 55,000, 46,000 and 35,000. immunoprecipitation experiments confirmed that the surface of muscle larvae share antigenic epitopes with antigens contained within and secreted by the stichosome. ctab antigen prepa ... | 1986 | 2433672 |
| chemical composition of newborn larvae, muscle larvae and adult trichinella spiralis. | 1986 | 2430908 | |
| mast cells in the regulation of intestinal electrolyte transport. | experiments were performed to determine the ionic basis and physiological messengers of transepithelial ion transport alterations (short-circuit current, isc) measured during the induction of intestinal anaphylaxis in an ussing chamber. antigens derived from trichinella spiralis, an intestinal parasite, were used to challenge jejunal tissue from guinea pigs immunized by infection with the parasite. histamine (10(-4) m) caused an increased in isc that was similar to that induced by antigen. diphe ... | 1986 | 2426973 |
| muscle fiber selectivity of trichinella spiralis and trichinella pseudospiralis (1983). | the biceps, semimembranosus, biceps femoris, and soleus muscles of female rockland wistar mice infected with either 1,000 trichinella spiralis or 1,000 trichinella pseudospiralis larvae were removed on days 12, 14, 16, and 18 post-infection (pi), sectioned and stained histochemically for their myosin atpase activity. light microscopic examination of the sections revealed that larvae of t. spiralis invade only the slow twitch muscle fibers, and those of t. pseudospiralis invade both the fast twit ... | 1986 | 2426435 |
| antigen-specific t-cell lines transfer protective immunity against trichinella spiralis in vivo. | t-cell lines specific for infective muscle larvae antigens of the intestinal nematode trichinella spiralis have been generated in vitro. these antigen-specific t-cell lines express the l3t4+ ly2- phenotype and secrete the lymphokines il-2, il-3 and gamma-ifn. they are stable in culture for up to 15 weeks and are protective when adoptively transferred into naive recipients. as few as 2 x 10(5) t. spiralis-specific tract. in addition, intestinal mastocytosis and peripheral blood eosinophilia were ... | 1986 | 2423438 |
| molecular identity of a major antigen of schistosoma mansoni which cross-reacts with trichinella spiralis and fasciola hepatica. | a glycoprotein cross-reactive among schistosoma mansoni, trichinella spiralis and fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against s. mansoni glycoproteins. four monoclonal antibodies recognized the same antigen which was one of the major s. mansoni glycoproteins precipitated by sera of hosts infected with either s. mansoni or t. spiralis. this antigen was expressed in s. mansoni cercariae, adult male and female worms, and eggs, and in s. haemat ... | 1986 | 2421229 |
| anamnestic stimulus-specific myoelectric responses associated with intestinal immunity in the rat. | changes in small intestinal motility reflective of local anaphylaxis are hypothesized to be associated with the rapid, immune-mediated rejection of infective larvae of the nematode trichinella spiralis. this hypothesis was tested by comparing intestinal myoelectric activity in primarily and secondarily infected rats; the latter group represented immunized hosts, i.e., immunized by initial contact with the parasite. patterns of slow waves, action potentials, interdigestive migrating myoelectric c ... | 1986 | 2420191 |
| partial characterization of two antigens secreted by l1 larvae of trichinella spiralis. | two protein antigens were isolated from excretory-secretory products of trichinella spiralis by biochemical methods and characterized with respect to their chemical and immunological properties. one antigen, of apparent mr 43,000, is an abundant secreted protein of infective l1 larvae, while the other, of 45-50 kda, is present in smaller amounts. yields, extinction coefficients, isoelectric points, amino acid compositions, and partial n-terminal amino acid sequences for each are reported. partia ... | 1990 | 2398916 |
| biochemical effects of luxabendazole on trichinella spiralis. | biochemical changes produced by luxabendazole in muscle-stage trichinella spiralis larvae consisted of a decrease in free glucose and glycogen levels (46.71% and 35.66%, respectively) after in vivo treatment, slight in vitro inhibition of fumarate reductase activity (24.15%) and, finally, inhibition of [3h]-colchicine-tubulin binding, which was found to be of a competitive nature, with an inhibition constant (ki) of 0.9 x 10(-7) m. in a parallel study, luxabendazole did not appear to be inhibito ... | 1990 | 2381894 |
| asymmetric fab glycosylation in guinea-pig igg1 and igg2. | the presence of asymmetric antibody molecules has been investigated in both igg1 and igg2 subclasses of guinea-pig immunoglobulins. it was found that about 20% of the igg1 and 10% of the igg2 were of asymmetric type. the proportion was essentially the same in the sera of normal animals, animals hyperimmunized with dinitrophenyl-bovine gamma globulin (dnp-bgg) and freund's adjuvant, and animals infected with trichinella spiralis. in the case of animals immunized with dnp-bgg, no differences were ... | 1990 | 2379937 |
| pathogenesis and serodiagnosis of experimental trichinella spiralis spiralis and trichinella spiralis nativa infections in cattle. | trichinella spiralis spiralis infections were established in cattle by gavage and by feeding infected musculature in the ration. trichinae were present in greatest numbers in masseter, tongue and diaphragm. trichinella spiralis nativa had a low infectivity to cattle although a light infection was established in one cow by a heavy challenge. cattle had an aversion to eating musculature unless it was camouflaged with molasses. clinical signs of reluctance to eat and masticate were observed between ... | 1990 | 2379115 |
| survey of trichinosis in breeding and cull swine, using an enzyme-linked immunosorbent assay. | serum samples obtained from 40,927 swine at various locations in north carolina between aug 1, 1987 and july 31, 1988, were tested for antibodies to trichinella spiralis, using an elisa based on a larval t spiralis excretory-secretory antigen. in the elisa, samples were considered to have positive results if the optical density (od) reading was equal to or 5 times greater than the mean od value of 4 negative-control sera from trichina-free swine. of the 40,927 serum samples tested, 154 (0.38%) w ... | 1990 | 2368950 |
| [preparation of monoclonal antibodies against trichinella spiralis and localization of antigens]. | balb/c mice were infected per os with the infective larvae (l1) of trichinella spiralis, and challenged by injecting 0.2ml soluble l1 antigen 3 days before cell fusion. sp2/0 myeloma and immune spleen cells were fused at the ratio of 1:5 in the presence 30%peg (mw 4,000). the fusion rate and antibody positive rate were 92.1% and 16.6% respectively using elisa. among these the chromosomes of 4 strains were 2n greater than 100 and that of the sp2/0 myeloma cells 2n less than or equal to 70. the ti ... | 1990 | 2365331 |
| infectivity, antigenicity and host responses to isolates of the genus trichinella. | comparisons were made of the infectivity and antigenicity of 4 trichinella spiralis isolates (s, d, y, w), of quite different geographical origins, and t. pseudospiralis (p) in rapid- and slow-responder inbred mice. infectivity was measured by the index of reproductive capacity (icr) expressed as the ratio between the number of muscle larvae recovered on day 30 post-infection (p.i.) and the numbers of larvae given at infection. antigen recognition was measured by the degree of proliferation of m ... | 1990 | 2362774 |
| zinc deficiency and zinc repletion: effect on the response of rats to infection with trichinella spiralis. | the expulsion of a primary infection of trichinella spiralis was studied in rats fed diets containing (per kg diet) either 3 mg zn [zinc deficient (zn-)] or 40 mg zn [zinc adequate (zn+)]. a dose of 2000 muscle larvae (ml) impaired weight gain (mg/g body wt) in all groups compared with uninfected controls [eg, 0-7 d postinfection (dpi): infected zn-, -105 +/- 10 (means +/- sem); uninfected zn-, 54 +/- 19 (p less than 0.001)]. in a study with 20.5 ml/g body wt, some zn- rats were transferred at t ... | 1990 | 2360546 |
| the intestinal gland of trichinella spiralis with emphasis on morphology and antigenicity. | the intestinal gland of muscle larvae and adult worms of trichinella spiralis was investigated with emphasis on its morphology and antigenicity. the gland is situated at the junction between the stichosome and the ampullar portion of the midgut. the cytoplasm was characterized by the presence of cytoplasmic granules. the granules, measuring 1 micron at maximum, were round or sometimes irregular in shape, and of homogeneous appearance with medium to high electron density. rough endoplasmic reticu ... | 1990 | 2358771 |
| the role of mucus in antibody-mediated rapid expulsion of trichinella spiralis in suckling rats. | rat pups suckling dams parasitized by trichinella spiralis express rapid expulsion, a protective response that is associated with the entrapment of infectious muscle larvae in intestinal mucus. immunofluorescent studies revealed that antibodies were bound to the surfaces of the entrapped larvae. mucus binding and rapid expulsion occurred in normal pups dosed with larvae coated with antibodies prepared from infected rat serum. subsequent experiments revealed that entrapped larvae escaped from muc ... | 1990 | 2354857 |
| synergistic interaction between immune serum and thoracic duct cells in the adoptive transfer of rapid expulsion of trichinella spiralis in adult rats. | rapid expulsion of trichinella spiralis could be transferred to naive adult rats with thoracic duct lymphocytes and immune serum. thoracic duct cells collected from days 3-5 and immune serum collected on day 28, respectively, after infection were effective. both cells and serum were unable to transfer rapid expulsion when given alone, even in large volumes. recipients of immune serum and cells eliminated a significantly higher number of larvae than control rats by 1 hr after challenge with muscl ... | 1990 | 2354718 |
| trichinella spiralis: secreted antigen of the infective l1 larva localizes to the cytoplasm and nucleoplasm of infected host cells. | antibodies were elicited against a purified antigen with an apparent molecular weight of 43k. this antibody preparation also detected a second antigen consisting of a group of closely related components of 45-50k. these antigens are stage specific for the infective first stage larva of trichinella spiralis and are among the repertoire of secreted antigens originating from the stichosome. antibody raised against the 43k antigen reacted with the stichosome and cuticle of the mature larva and the c ... | 1990 | 2354715 |
| trichinella spiralis in an agricultural ecosystem: transmission in the rat population. | four hundred forty-three norway rats (rattus norvegicus) were examined to determine their role in the transmission and maintenance of trichinella spiralis on a pig farm. rats, classified by sex and weight, were examined for trichinellosis by peptic digestion of muscle samples. over a 25-mo period, 188 (42.4%) rats were found to be infected with t. spiralis. the mean intensity of infection was 293.2 larvae per gram (lpg) of muscle; 65 (34.6%) infected rats had intensities of infection greater tha ... | 1990 | 2352066 |
| [epidemiology of alveolar echinococcosis in france. 1. intestinal helminths in the red fox (vulpes vulpes l.) from haute-savoie]. | in the course of an epidemiological survey concerning the alveolar echinococcosis and its causative agent, echinococcus multilocularis, the autopsies of 150 wild red foxes captured in the department of the haute-savoie between december 1983 and august 1988 disclosed 139 cases of intestinal helminthiasis with cestodes and nematodes; no trematode was found. multiple infections were frequent. the cestoda recorded consist of the following species: taenia crassiceps: 44 cases (29%); echinococcus mult ... | 1990 | 2344133 |
| effect of some drugs on some histopathological and immunological aspects of experimental trichinosis in albino rats. | the present work is concerned with study of the effects of praziquantel, thiabendazole, mebendazole, cyclophosphamide and cortisone on histopathology of the small intestine (during intestinal phase) and skeletal muscles (during muscular phase), in addition to t. lymphocytes count and serum igg estimation in experimental trichinosis in albino rats. praziquantel has no effect on the histopathology of small intestine or skeletal muscles. thiabendazole and mebendazole treatment resulted in complete ... | 1990 | 2332669 |
| role of t cell subsets in the maternal-to-neonatal transmission of immunity against trichinella spiralis during lactation in rats. | we have previously demonstrated the maternal-to-neonatal transfer of immunity to t. spiralis during lactation and have shown that antigen-specific t lymphocytes, when injected into the mother or orally fed to neonates, can mediate this transfer. to further analyze the t cell subsets involved in conferring this protection, t lymphocytes were isolated from the mesenteric lymph nodes of syngeneic donor rats infected 4-6 days earlier with t. spiralis. the t cells were incubated in vitro with either ... | 1990 | 2329549 |
| trichinella spiralis: altered expression of muscle proteins in trichinosis. | mammalian muscle undergoes significant alterations morphologically, ultrastructurally, and biochemically following infection by trichinella spiralis larvae. to investigate this host/parasite relationship in more detail, a new method to isolate t. spiralis-infected cells (nurse cells) in preparative quantities was developed. nurse cells isolated by sequential protease treatments contain larvae and retain many of the characteristics of those in situ. when analyzed by sds-page, a wide range of prot ... | 1990 | 2323397 |
| trichinella spiralis: major histocompatibility complex-associated elimination of encysted muscle larvae in swine. | a heretofore undescribed host-mediated reactivity against encapsulated muscle larvae (ml) of the nematode trichinella spiralis is reported. inbred miniature swine (nih minipigs) of three independent sla phenotypes, which received a primary oral dose of 300 t. spiralis ml, successfully resisted a secondary infection of 10,000 ml; however, only pigs of the slaa/a phenotype exhibited an unusual and highly significant reduction in the numbers of encysted ml from the primary infection (p less than 0. ... | 1990 | 2323396 |
| a spectrum of antibody response with time after trichinella spiralis infection in rats. | a chronology of class-specific antibody response against the trichinella spiralis infection in fischer rats was investigated. g-class antibodies against the cuticle inner layer(s), hypodermis, hemolymph, glycogen aggregates, discrete areas in genital primordial cells, intestinal gland cell granules, and cytoplasmic granules in the cords were detectable 2 wk after infection (the rapid-responding group), whereas g-class antibodies against the cuticle surface, stichocyte granules, and the esophagus ... | 1990 | 2319424 |
| effect of high temperature on infectivity of toxoplasma gondii tissue cysts in pork. | to study the effect of high temperature on infectivity of toxoplasma gondii tissue cysts, pork from infected pigs was mixed with infected mouse brains and homogenized thoroughly. twenty-gram samples of infected homogenized meat were sealed in plastic pouches, pressed to a uniform thickness of 2 mm, and subjected to water-bath temperatures of 49, 52, 55, 58, 61, 64, and 67 c for 0.01, 3, 6, 9, 12, 24, 48, and 96 min. treated samples were digested in hcl-pepsin solution and bioassayed in mice. tox ... | 1990 | 2319420 |
| a thymus-independent (type 1) phosphorylcholine antigen isolated from trichinella spiralis protects mice against pneumococcal infection. | a phosphorylcholine (pc)-containing glycoprotein of 68,000 molecular weight (mw) was isolated from trichinella spiralis. the potential of this antigen (tsp) as a species-specific vaccine against streptococcus pneumoniae was studied in both immunologically deficient (cba/n) and normal (cfw) mice. unlike the pc determinant found in s. pneumoniae, tsp is a type 1 thymus-independent (ti-1) antigen, as it was able to stimulate pc-specific antibody production in cba/n animals, though less well than in ... | 1990 | 2312167 |
| trichinella spiralis: murine strain variation in response to monoclonally defined, protective, nonstage-specific antigens. | nine hybridoma cell lines secreting monoclonal antibodies (mabs) against trichinella spiralis muscle larvae (ml) excretory/secretory antigens (esa) were developed. two mabs, 6-d8-e3 (6d8) and 6-b1-g10 (6b1), were studied in detail. western blot analysis using ml esa showed that 6d8 recognized 35- and 40-kda constituents whereas 6b1 identified a doublet of 33 kda. however, western blots of sds-page of crude ml homogenate showed that 6d8 identified proteins of approximately 35 and 43-60 kda, where ... | 1990 | 2311715 |
| trichinella spiralis: the in situ localization of muscle larva antigens recognized by humans. | 1990 | 2295323 | |
| a tube latex test based on colour separation for the detection of igm antibodies to either one of two different microorganisms. | a simple two stage assay was developed for the detection of igm antibodies to either one of two microorganisms chosen arbitrarily for this study. salmonella enteritidis and trichinella spiralis. in the first stage, magnetic polystyrene beads (dynabeads) coated with anti-mu (mouse) antibodies were incubated with the test material for 45 min to capture the igm antibodies. in the second stage, indicator latex particles were incubated with the dynabeads for 30 min and the results read following sett ... | 1990 | 2273269 |
| molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae. | a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ... | 1990 | 2270099 |
| isolation of trichinella-specific antigens for diagnosis by gradient monoclonal antibody affinity chromatography. | monoclonal antibodies were generated for the isolation of specific antigens from trichinella spiralis. two monoclonal antibodies (7g6-2 and 10b6-1) of class igg2b and igg1 were selected according to their reactivities in an enzyme-linked immunosorbent assay and western blot. clone 7g6-2 reacted with an antigen with molecular mass of approximately 60 kda, and clone 10b6-1 bound to multiple antigens ranging from 49 to 62 kda on western blot. antibodies of each clone were purified partially from mo ... | 1990 | 2254818 |
| helminth parasitism in martens (martes americana) and ermines (mustela erminea) from washington, with comments on the distribution of trichinella spiralis. | helminths are reported for the first time from ermines (mustela erminea) and martens (martes americana) in washington (usa). among 22 adult ermines, 41% were infected by one or more of five species (taenia mustelae, alaria mustelae, molineus patens, m. mustelae and trichinella spiralis). among 78 adult martens from three geographic localities, the prevalence was 83%. nine species were identified (mesocestoides sp., t. mustelae and t. martis americana, euryhelmis squamula, m. patens, baylisascari ... | 1990 | 2250320 |
| effect of t. spiralis infection on intestinal motor activity in the fasted state. | we sought to determine the effects of trichinella spiralis infection on small intestinal motor activity in the fasted state in dogs and relate it to clinical symptoms during the intestinal phase of trichinosis. motor activity was recorded by strain gauge force transducers. infection with t. spiralis resulted in a significant increase in the incidence and proximal origination of giant migrating contractions (gmcs) during the first 5 days postinfection. this was also the time when the dogs had dia ... | 1990 | 2240213 |
| pattern of eosinophilia and muscle larval burdens in experimental trichinosis. | absolute eosinophilic counts to different developmental stages of trichinella spiralis in experimental trichinosis were studied to identify the stage responsible for eosinophilia. two peaks of eosinophilia were noticed, the first (545 +/- 172.3) was attributed to the adult stage while the migrating larvae were accused to be responsible for the more pronounced second peak (1265 +/- 331.7). with encapsulation a steady raised level was demonstrated. chromatographic studies to determine the nature o ... | 1990 | 2230342 |
| 3-methylcholanthrene-induced immunosuppression in mice to trichinella spiralis antigens. | the immunosuppressive effects of in vivo (subcutaneous) exposure to 40 or 80 mg/kg 3-methylcholanthrene (mc) were examined in aryl hydrocarbon hydroxylase (ahh) responsive c57bl/6 (b6) and ahh non-responsive dba/2 (d2) inbred strains of mice. twenty-four hours after treatment with carcinogen or vehicle alone, animals were primed with crude l1 muscle larvae antigen from t. spiralis. immune status was assessed in vitro after six days as antigen-specific lymphoproliferation. the proliferation of sp ... | 1990 | 2229923 |
| [acute trichinosis in children. intrafamilial epidemic outbreak in mexico city]. | four cases of trichinosis found in a family living in iztapalapa, mexico, d.f. are reported. the family reported to frequently eat raw sausage originally from villanueva, zacatecas from which trichinella spiralis larvae were isolated. diagnosis was difficult in the first case since no epidemiological history was associated with the patient, besides the fact that the patient showed no signs of eosinophilia and blood tests were negative. the diagnosis in this case was confirmed through muscular bi ... | 1990 | 2222824 |
| genetic analysis of the relationship between interleukin production and worm rejection in trichinella spiralis-infected inbred mice. | the production of interleukin 1 (il-1), il-2, and il-3 by peritoneal macrophages, mesenteric lymph node (mln), or spleen cells from inbred strains of mice infected with trichinella spiralis was examined. the mice belonged to the worm rejection phenotypes previously characterized as strong (nfs), intermediate (c3h, bub, dba/1, swr, cba, etc.), or weak (b10.q, b10.br, etc.). strong responder nfs mice produced approximately twice as much il-1 as intermediate responder c3heb/fe or weak responder b10 ... | 1990 | 2213413 |
| immunocytochemical evaluation of trichinella spiralis larval antigen. | the antigenicity of trichinella spiralis muscle larvae was studied for the purpose of developing an immunodiagnostic test for trichinosis. ultrathin sections of fixed larvae were treated with sera from patients with a variety of parasitic infections including trichinosis, anisakiasis, gnathostomiasis, fascioliasis, dirofilariasis, severe trichuriasis, moderate trichuriasis, paragonimiasis, cysticercosis cellulosae hominis and diphyllobothriasis, and then treated with protein a-tagged colloidal g ... | 1990 | 2212922 |
| trichinosis in a dog. | trichinella spiralis infection was identified by direct fecal examination as the cause of gastrointestinal disease in a dog. the source of infection was believed to be a woodchuck. management included supportive care and benzimidazole treatment. vomiting, diarrhea, anorexia, and myalgia are the main signs of trichinosis, but routine fecal examination seldom reveals t spiralis in natural infections. mebendazole is the recommended anthelmintic and should be used to eliminate intestinal larvae and ... | 1990 | 2211292 |
| antigen-antibody complexes in experimental infection with trichinella spiralis and their role in the development of kidney lesions. | the kidneys of rats, experimentally infected with trichinella spiralis were studied by means of light and fluorescent microscopy. the results obtained suggest that this is a case of a proliferative intra- and extracapillary nephritis. highest intensity of the pathological process was noted between the 30th and 50th day of the infection. the immunofluorescent reaction of kidney glomeruli demonstrated that the immune complexes were located predominantly in the basal membrane and the mesangium. the ... | 1990 | 2186668 |
| distribution of intestinal mast cell proteinase in blood and tissues of normal and trichinella-infected mice. | a sensitive and specific enzyme-linked immunosorbent assay (elisa) was developed for mouse intestinal mast cell proteinase (imcp). specificity was demonstrated by the absence of immunoreactivity with extracts of isolated serosal mast cells (smc), or with high concentrations (50 micrograms/ml) of the antigenically similar rat mast cell proteinases i or ii. the small and large intestines in normal mice were the major sources of imcp, there being little or no imcp in non-mucosal tissues. concentrat ... | 1990 | 2179829 |
| the effects of ethanol consumption on the expression of immunity to trichinella spiralis in rats. | the effects of ethanol ingestion on immune responses of female rats against trichinella spiralis (t. spiralis) infections were investigated. female rats were pair-fed either ethanol-containing or isocaloric control liquid diets for 68 days, during which time they underwent one pregnancy cycle. for some studies, animals were fed ethanol diets for 68 days beginning prior to pregnancy and continuing through lactation and involution. they were infected on day 7 of involution with 1000 l1 larvae of t ... | 1990 | 2178479 |
| altered smooth muscle function in worm-free gut regions of trichinella-infected rats. | we have extended a recent study demonstrating increased contractility of jejunal smooth muscle of rats infected with the nematode parasite trichinella spiralis, the primary habitat of which is the jejunum. in this study, muscle from the worm-free ileum of infected rats showed decreased contractility compared with control, illustrating regional differences in muscle responses to the infection. however, in jejunal muscle from worm-free segments that had been excluded from the rest of the gut befor ... | 1990 | 2166442 |
| alterations in the longevity and fecundity of adult trichinella pseudospiralis related to method of isolation of infective larvae. | the infectivity of trichinella pseudospiralis infective larvae was reduced significantly following exposure to low ph or a combination of 1% pepsin at low ph compared to that for larvae isolated in phosphate-buffered saline (pbs) at ph 7.0. reduction of host gastric ph by administration to mice of sodium bicarbonate solution in pbs was accompanied by an increase in the infectivity of larvae isolated in 1% pepsin/hcl (p/hcl) compared to that for worms inoculated into hosts given pbs alone. fewer ... | 1990 | 2161917 |
| t-helper subset function in the gut of rats: differential stimulation of eosinophils, mucosal mast cells and antibody-forming cells by ox8- ox22- and ox8- ox22+ cells. | thoracic duct lymphocytes (tdl) collected 3 days after infection of rats with trichinella spiralis (ts) and adoptively transferred into normal, uninfected recipients, increased the numbers of both mucosal mast cells (mmc) and eosinophils (eos) in the intestine. the cd4+ t-helper cell population was separated into two subsets (ox22+ and ox22-) using ox22 monoclonal antibody (mab) and panning techniques. after adoptive transfer of these t-helper subsets i.v., rats were challenged with ts 24 hr lat ... | 1990 | 2146212 |
| intraintestinal migration to the epithelium of protective, dividing, anti-trichinella spiralis cd4+ ox22- cells requires mhc class ii compatibility. | we have measured the intraintestinal migration of activated th cells belonging to the ox8- ox22- and ox8- ox22+ subsets derived from thoracic duct lymph of rats infected with trichinella spiralis. cells in s-phase were labeled with 125i-udr or 3h-tdr in vitro and transfused i.v. identical proportions of both helper cell subsets localized in the small intestine but three to four times as many ox22- cells as ox22+ cells migrated to the intestinal epithelium. experiments using mhc class i and ii re ... | 1990 | 2142708 |
| dna analysis in the diagnosis of infection and in the speciation of nematode parasites. | dna analysis is playing an increasingly important role in characterising and classifying nematode parasites. though less emphasis has been placed on utilising dna elements to study nematodes of veterinary importance, correct diagnosis is, nevertheless, critical to proper treatment and control. the genus trichinella presents a particularly interesting problem since the level of classification within this genus remains unclear. herein we discuss the application of dna analysis to the diagnosis and ... | 1990 | 2132695 |
| immunodiagnosis of nematode infections and prospects for vaccination, with special reference to trichinella spiralis. | within the phylum nematoda are species not only responsible for several chronic, debilitating diseases of man, but also for significant economic losses resulting from parasitaemias of livestock. parasitic zoonoses caused by nematodes, such as trichinosis, present a public health concern as well. in an effort to combat these diseases, much research has been directed towards the development of vaccines that will protect man and animals from selected parasitic infections. concomitantly, sensitive, ... | 1990 | 2132694 |
| immunotoxicity of bis(tri-n-butyltin)oxide in the rat: effects on thymus-dependent immunity and on nonspecific resistance following long-term exposure in young versus aged rats. | to investigate whether immune function suppression observed in an earlier study after short-term bis(tri-n-butyltin)oxide (tbto) exposure also occurred after long-term treatment, function studies for specific and nonspecific resistance were performed after exposure of weaned male rats to diets containing 0, 0.5, 5, or 50 mg tbto/kg for 4-6 and 15-17 months. treatment for 4.5 months had no effect on body weight but reduced thymus weight at 50 mg/kg. regarding the thymus-dependent immunity, delaye ... | 1990 | 2118283 |
| involvement of type i hypersensitivity in rapid rejection of trichinella spiralis from adult rats. | the role of type i hypersensitivity in the rapid rejection of trichinella spiralis from actively and passively immunized rats was examined. net intestinal fluid secretion, which occurs during the rejection of the parasites, was used to verify the expression of local anaphylaxis and was examined for its possible role in the rejection process. worm establishment in the small intestine 30 min after intraduodenal inoculation was significantly reduced in rats that were passively immunized with immune ... | 1990 | 2099353 |
| infectivity, reproductive capacity and distribution of trichinella spiralis and t. pseudospiralis larvae in experimentally infected sheep. | twelve merino sheep were experimentally shown to be susceptible to infection with trichinella spiralis or t. pseudospiralis by feeding on infected carcasses of mice or by oral intubation with recovered muscle larvae. the larvae recovered from the sheep showed variable tissue distribution. the diaphragm and tongue were most affected. the viability of the recovered larvae was confirmed by successful passage in mice. the reproductive capacity of t. spiralis in sheep was higher than that of t. pseud ... | 1990 | 2094791 |
| functional correlations between mucosal mast cell activity and immunity to trichinella spiralis in high and low responder mice. | levels of intestinal mast cell protease (imcp) were quantified in serum, gut tissue and in intestinal fluids taken from mice infected with trichinella spiralis during primary and secondary infections. the ability to generate a mast cell response was dependent on the response phenotype of the mouse strain used. the mast cell response in rapid responder mice (nih) occurred sooner and was more pronounced than in either intermediate (swr) and low responder (b1o) mice. this pattern was also reflected ... | 1990 | 2084610 |
| catecholaminergic neurons in trichinella spiralis (nematoda). | 1991 | 2047375 | |
| isolation of specific antigens from trichinella spiralis by the rotating horizontal ampholine column method. | by preparative isoelectric focusing in a rotating ampholine column, specific antigens with molecular weights of 45, 47 and 53 kda were successfully isolated from crude somatic extracts of trichinella spiralis muscle larvae. these antigens, with pi 5.5, 4.3 and 4.4, were found to possess the same epitope found in specific antigens in the excretory/secretory products that were recovered by in vitro culture of muscle larvae. when the antigens obtained by the two methods were compared using enzyme-l ... | 1991 | 2047374 |
| sequelae after infection with trichinella spiralis: a prospective cohort study. | it is still a matter of debate whether or not infection with trichinella spiralis is followed by long lasting sequelae. a common source epidemic of trichinosis which occurred in bitburg, frg, in october 1982 gave us the opportunity of performing a controlled cohort study (171 patients and 51 controls). 145 patients and 44 controls completed the 3 year follow-up. complaints persisted in 36% of patients aged less than 30 years and in 100% of patients aged greater than 50 years. complaints in order ... | 1991 | 2042369 |
| procedure for selecting monoclonal antibodies for use in a ligand displacement assay of serum antibody levels. | monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. a screening process is described which involves measurement of displacement of antibody by an elisa procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody ... | 1991 | 2040708 |
| food safety. trichinella spiralis infection. | 1991 | 2039687 | |
| cloning and expression of complementary dna encoding an antigen of trichinella spiralis. | complementary dna (cdna) encoding a 49-kda antigen of trichinella spiralis was cloned, characterized, and expressed in escherichia coli by recombinant dna methods. as a first step, 29 residues of n-terminal amino acid sequence were determined by direct analysis of highly purified antigen. mixed-sequence primers based on the amino acid sequence were then synthesized and used as primers to generate a partial cdna by the polymerase chain reaction (pcr). a nearly full-length cdna was then obtained b ... | 1991 | 2038363 |
| development of a serologic assay for cysticercosis, using an antigen isolated from taenia spp cyst fluid. | an ammonium sulfate-soluble fraction of taenia hydatigena cyst fluid (thfas) was further evaluated for use in the immunodiagnosis of cysticercosis. analysis of thfas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and protein immunoblot analysis confirmed earlier reports of a highly specific, low molecular weight antigen in this preparation; in contrast, other components of thfas were shown to react nonspecifically. antibodies against the less than 12-kd diagnostic antigen were dete ... | 1991 | 2035923 |
| detection of repetitive sequences of trichinella spiralis by the polymerase chain reaction in experimentally infected mice. | 1991 | 2027886 | |
| a role for ige in intestinal immunity. expression of rapid expulsion of trichinella spiralis in rats transfused with ige and thoracic duct lymphocytes. | in these experiments we characterize the protective antibodies in immune serum that interact synergistically with immune thoracic duct lymphocytes (tdl) to induce rapid expulsion (re) of trichinella spiralis in adult rats. antibodies with both reaginic and nonreaginic activity mediated re upon passive transfer to adult rats that had been adoptively transfused with immune tdl 7 days earlier. in serum collected 28 days after a primary infection, the most important antibody was homocytotropic ige. ... | 1991 | 2026881 |
| trichinella spp.: differential expression of acid phosphatase and myofibrillar proteins in infected muscle cells. | major alterations are induced in muscle cells infected by either trichinella spiralis or trichinella pseudospiralis. to investigate the response of muscle to these infections we have analyzed the expression of acid phosphatase (acp, ec 3.1.3.2), adult skeletal muscle myosin heavy chain, and muscle tropomyosin proteins in infected mouse skeletal muscle cells. using t. spiralis-infected cells, we provide strong evidence that the tartrate-sensitive acp of these cells was synthesized by the infected ... | 1991 | 2015871 |
| changes in nurse cell nuclei during synchronous infection with trichinella spiralis. | the rate of enlargement of nuclei was determined on 4-microns-thick sections of synchronously infected mouse thigh muscle. normal muscle nuclei had a geometric mean volume of 84 microns and a range of 42-170 microns 3. at days 5, 6, 7, 8, and 10 and 6 mo after infection, mean nuclear volume was 177 (100-315) microns 3, 254 (140-462) microns 3, 278 (172-447) microns 3, 681 (407-1,138) microns 3, 512 (326-804) microns 3, and 509 (298-870) microns 3, respectively. size of nuclei for any given day f ... | 1991 | 2010862 |
| blood vessels in trichinella spiralis infections: a study using vascular casts. | larvae of trichinella spiralis initiate the transformation of myocytes into nurse cells that become surrounded by elaborate networks of blood vessels. to examine the structure of these networks (i.e., retes), transcardic perfusion was used to clear the vascular tree of trichinella-infected mice and to inject a plastic that polymerized in situ. vascular complexes were found only around infected myocytes and were characterized by large circumferential vessels that give rise to the smaller channels ... | 1991 | 1992100 |
| a dot-elisa mimicry western blot test for the detection of swine trichinellosis. | a dot enzyme-linked immunosorbent assay (dot-elisa) using antigens purified by monoclonal antibody affinity chromatography was developed for detecting trichinella spiralis infection in swine. the test was as sensitive as an elisa using excretory-secretory products as antigen and western blot analysis, and nearly as specific as the western blot. the dot-elisa detected all of 20 low infections (0.08-4.74 larvae per gram of diaphragm), most of them by 5-6 wk postinfection. sera from 1,960 farm-rear ... | 1991 | 1992098 |
| the surface lipid of parasitic nematodes: organization, and modifications during transition to the mammalian host environment. | the biophysical properties of the surface lipid of a range of nematode species and their developmental stages were examined, using fluorescent lipid probes and fluorescence recovery after photobleaching (frap). these methods can be applied to living, intact parasites, and the analysis confined to lipid on the outermost surface. in all cases, surface lipid was unusual in its selectivity for the insertion of the lipid probes. in addition, a polar lipid probe was generally not free to diffuse in th ... | 1990 | 1978532 |
| peripheral blood mononuclear cell subsets during trichinella spiralis infection in pigs. | the immune response of 'yugoslav meat breed' pigs inoculated with low doses of trichinella spiralis muscle larvae was followed over two to nine weeks of primary infection, by analysing changes in peripheral blood mononuclear cell subsets, the development of a humoral antibody response and muscle larvae burden. during the course of the infection, infected animals showed a persistent elevation of both cd4+ and cd8+ t cell subsets from days 15 to 60 after the parasite exposure. during this time, th ... | 1990 | 1974364 |
| [differentiation of the sex of trichinella larvae collected in changchun]. | the length of the rectum in male trichinella spiralis larvae is about twice that in female larvae, being 54.39 (50.4-63.0) microns in males and 28.05 (18.9-31.35) microns in females. the gonad rudiment of the female larvae has already differentiated obviously into seminal receptacle rudiment and uterus rudiment, while that of the male larvae remains undifferentiated. the seminal receptacle rudiment is a granular, small mass and dark brown in color, while the uterus rudiment is composed of a colu ... | 1991 | 1959180 |
| igg response in guinea pigs to trichinella spiralis infection. | an enzyme-linked immunosorbent assay (elisa) using excretory-secretory antigens was developed to study the dynamics of the igg antibody response to varying levels of trichinella spiralis infection in the guinea pig. four groups of four hartley guinea pigs each were infected with 1250, 250, 50 or 10 t. spiralis infective muscle larvae. they were bled every 15 days for 6 months and the igg antibody response determined by elisa. the time of seroconversion was dose dependent as the larger the dose, ... | 1991 | 1957490 |
| humoral immune responses in human infection with the whipworm trichuris trichiura. | the humoral immune response to infection with trichuris trichirua was investigated by elisa and immunoblotting using human sera from the caribbean island of st lucia. immunoblot analysis of the degree of cross-reactivity with the related trichuroid trichinella spiralis and with the other commonly co-existent nematodes, ascaris lumbricoides and toxocara canis, was carried out using selected sera. the igm, iga, ige, and igg subclass antibody levels were measured in elisa using a detergent solubili ... | 1991 | 1956697 |
| immunoregulation of endometrial and jejunal epithelia sensitized by infection. | the hypothesis was tested that the uterus of the rat orally infected with the parasite trichinella spiralis becomes hypersensitized and that subsequent antigenic challenge affects functions in the endometrial epithelium. results of experiments comparing the immunological responsiveness of isolated rat uterus with that of the jejunum supports our hypothesis. antigenic challenge of uterus mounted in ussing-type chambers causes an elevation in transuterine short circuit current (isc) of 6.4 +/- 0.8 ... | 1991 | 1937920 |
| helminthotoxic responses of intestinal eosinophils to trichinella spiralis newborn larvae. | because the gastrointestinal lamina propria is the first line of defense against invasion with trichinella spiralis newborn larvae, we investigated the helminthotoxic characteristics of isolated lamina propria eosinophils. eosinophils were isolated from the intestinal lamina propria of rats and purified to nearly 90% purity by a combination of velocity sedimentation through percoll and unit gravity sedimentation through a continuous gradient of bovine serum albumin. isolated eosinophils were of ... | 1991 | 1937799 |
| genetic control of the immune response to trichinella spiralis: recognition of muscle larval antigens. | host antibody recognition of muscle larval (ml) antigens of trichinella spiralis was examined. monoclonal antibodies (moabs) to known host protective ml antigens have been produced in order to aid this examination. eleven strains of mice with independent mhc haplotypes and seventeen t. spiralis infected human patients were all found to recognize the same three major antigens as the monoclonal antibodies; i.e., of mw 41, 46 and 55 kd. however all serum samples tested also recognized further ml an ... | 1991 | 1923564 |
| [cardiac trichinosis--echocardiographic study]. | trichinosis is an intestinal and tissue infection of man caused by trichinella spiralis. the most frequent and serious complication is myocarditis. m-mode and two-dimensional echocardiographic studies were carried out on 16 patients with clinical manifestation of trichinosis. the parameters of the left ventricular function, measured by echocardiography were examined, and the results were compared with the same parameters in 50 normal individuals. in 11 patients a variety of morphologic findings, ... | 1991 | 1916420 |
| interferon-gamma treatment in mice experimentally infected with trichinella spiralis. | interferon-tau (ifn-tau) treatment of trichinella spiralis-infected balb/c mice was investigated. the therapeutic regimen consisted of daily intraperitoneal injection of 10(4) u murine ifn-tau for 7 days, starting at 2 weeks post-infection. striated muscle samples (diaphragm, thigh) were collected at 4, 8 and 12 weeks after infection. the muscle larval burden, the degree of encystation and the digestion of t. spiralis larvae were investigated. furthermore, immunohistochemical studies of the infl ... | 1991 | 1909790 |
| immunization against schistosoma mansoni by using trichinella spiralis and trichinella pseudospiralis antigens. | the antigenic fraction of a cell free homogenate of each of trichinella spiralis and t. pseudospiralis were prepared to be used for induction of immunity against s. mansoni in albino mice. the results of the study showed that immunization with either of the two antigens induced reduction in worm load ranging from 80.48% to 87.86% in the different groups of the study. reduction in tissue egg count was also reported: liver egg count was reduced from 69.47% to 100%. intestinal egg count reduction r ... | 1991 | 1908495 |
| h-2 (i-a) control of the antibody repertoire to secreted antigens of trichinella spiralis in infection and its relevance to resistance and susceptibility. | humans infected with the parasitic nematode trichinella spiralis vary in the specificity of their antibody responses to the antigens of the parasite. the possibility that such host variation in antigen recognition has a genetic basis was examined in infected inbred mice whose antigen recognition profiles were characterized by immunoprecipitation of biosynthetically labelled secreted materials of adult parasites and sds-page. the strains varied considerably in repertoire and none produced detecta ... | 1991 | 1904401 |
| trichinella spiralis infection--united states, 1990. | since 1947, when the public health service began to record statistics on trichinosis, the number of cases reported by state health departments each year has declined: in the late 1940s, health departments reported an average of 400 cases and 10-15 deaths each year; from 1982 through 1986, the number declined to an average of 57 per year (figure 1) and a total of three deaths (1,2). although this trend reflects a decline in the number of cases related to commercially purchased pork, recent outbre ... | 1991 | 1898978 |
| preliminary characterization and interaction of tubulin from trichinella spiralis larvae with benzimidazole derivatives. | tubulin was estimated to account for 0.3% of the total soluble protein in trichinella spiralis cytosolic fractions. tubulin from t. spiralis was partially purified by precipitation with either taxol or vinblastine sulphate. immunoblotting with alpha- and beta-tubulin monoclonal antibodies revealed the presence of tubulin in t. spiralis partially purified preparations. electrophoretic mobility of t. spiralis tubulin in sodium dodecyl sulphate-polyacrylamide gels was very similar to that shown by ... | 1991 | 1897122 |
| an immunocytochemical analysis of a class-specific antibody response against trichinella spiralis in humans. | the response of different classes of antibodies against antigens of the muscle larvae of trichinella spiralis was tested using an immunocytochemical approach. ultrathin sections of resin-embedded larvae were treated with sera from patients with trichinosis, then exposed to a biotinated second antibody and stained with avidin-gold complex. antibody of the m-class was a major component in the response against a slow-responding group of antigens that included stichocyte granules, the cuticle surfac ... | 1991 | 1895030 |
| trichinella spiralis: light microscope monoclonal antibody localization and immunochemical characterization of phosphorylcholine and other antigens in the muscle larva. | a panel of monoclonal antibodies was used to examine the structure of the muscle larva of trichinella spiralis under the light microscope. immunofluorescence and, in some cases, immunoperoxidase staining were used. all four antibodies reacted with the cuticle of the organism, although differences in the staining pattern were observed for some of these. interestingly, all the antibodies also reacted with the stichosome. one of the antibodies (ts2ab) is specific for the hapten, phosphorylcholine. ... | 1991 | 1889472 |
| trichinella spiralis: a 76-kda excretory/secretory larval antigen identified by a monoclonal antibody. | spleen cells from balb/c mice were fused with myeloma cells following infection of the mice with trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. a monoclonal antibody (mab), designated as ts 3g6 which did not react with sera or tissue extracts from noninfected mice, rats, and guinea pigs, was selected for further studies because of its high activity and specificity. when tested in elisa ts 3g6 did not cross-react with ascaris suum, a. lumbricoides, toxocar ... | 1991 | 1889470 |
| testicular changes in rats after trichinella spiralis infection. | infection of rats with trichinella spiralis was found to be associated with testicular enlargement. a paucity of leydig cells with marked oedema of interstitial areas were observed. most of the seminiferous tubules were lined by spermatogonia and primary spermatocytes. t. spiralis larvae were not detected in the testes. using the indirect immunoperoxidase procedure, t. spiralis antigen was detected as weak brown staining. the antigen was diffusely distributed in the cytoplasm of spermatogenic ce ... | 1991 | 1875071 |
| [parasite fauna of red foxes in berlin (west)]. | the infestation with ecto- and endoparasites of red foxes in berlin (west) was investigated. as shown by post mortems of 100 animals (47 males and 53 females), none of them was found to be free of parasites. with regard to ectoparasites, 3 species of ixodes ticks, 7 flea species, one species of biting lice and mite species were demonstrated. the sarcoptic mange is often inducing the death of the foxes and is considered to be a limiting factor of the population increment. concerning endoparasites ... | 1991 | 1872791 |
| immunohistochemical comparison of t-cell and macrophage populations in mammary tissue of control and trichinella spiralis-infected rats. | we have shown that antigen-specific t lymphocytes can mediate maternal-to-neonatal immunity during lactation. present studies address the dynamics of lymphocyte accumulation in the mammary gland during normal and disease stimulated conditions. monoclonal antibodies specific for total t cells, suppressor/cytotoxic and helper subsets, and macrophages were used in conjunction with immunohistochemistry to identify and count the individual cell types. in unstimulated mammary tissue, following a rise ... | 1991 | 1867400 |
| trichinella spiralis infection alters small bowel motor activity in the fed state. | the effect of trichinella spiralis infection on small intestinal transit and motor activity in the fed state during the intestinal phase of infection was studied. contractions were recorded by strain gauge transducers, and mean transit time was measured by marker dilution technique. the mean amplitude and area of individual phasic contractions decreased, but no change occurred in their mean duration during trichinosis. the total amplitude and area of phasic contractions also decreased; this was ... | 1991 | 1860631 |
| immunity in swine inoculated with larvae or extracts of a pig isolate and a sylvatic isolate of trichinella spiralis. | inoculation of swine with a sylvatic isolate of trichinella spiralis, designated t s nativa, resulted in low numbers of muscle larvae, compared with muscle larvae accumulation in swine inoculated with a pig type of t s spiralis. despite low infectivity of t s nativa for swine, primary inoculation resulted in high levels of immunity against challenge infection with t s spiralis. this immunity was expressed in accelerated expulsion of challenge adults from the intestine and reduced numbers of musc ... | 1991 | 1854102 |
| immunotoxicity of 2-methoxyethanol following oral administration in fischer 344 rats. | the immunotoxicity of the glycol ether 2-methoxyethanol (me) was evaluated in adult fischer 344 rats using a variety of in vitro and in vivo immune function assays. in the first phase of this study, male rats were dosed by oral gavage with me in water, at dosages ranging from 50 to 200 mg/kg/day, for 10 consecutive days. decreases in thymus weights were observed at dosages of 50-200 mg/kg/day in the absence of decreased body weights. lymphoproliferative (lp) responses to concanavalin a and phyto ... | 1991 | 1853347 |
| [the reproductive activity of trichinella spiralis in the early stages of cultivation on artificial nutrient media]. | reproductive activity of mature trichinella spiralis was studied during its cultivation in three nutrient media. most active hatching of trichinella larvae was observed in erl's saline, less active in eagle's medium and in the lactalbumin hydrolyzate medium. in erl's medium the hatching of larvae accounted for 80% of their total mass for the first four hours of the experiment and the absolute number of larvae was 2.5 to 10 times greater than that in the two other media. the use of erl's medium m ... | 1991 | 1852481 |
| reconstruction of immune-mediated ion secretion in gut mucosa of the athymic rat. | a defect in an immune-mediated, physiological reaction in the small intestine of congenitally immunosuppressed rats has been identified and corrected by recoupling the immunological trigger in the mucosa with epithelial effector cells. antigenic challenge of jejunum from euthymic rats sensitized by infection with trichinella spiralis evoked an anaphylactic response in vitro that was characterized by an elevation in transmural short-circuit current (isc) and caused by net cl- secretion. in contra ... | 1991 | 1852477 |
| impaired sympathetic nerve function in the inflamed rat intestine. | the effect of intestinal inflammation on norepinephrine release from the myenteric plexus in the trichinella spiralis-infected rat was assessed. longitudinal muscle-myenteric plexus preparations were preincubated with [3h]norepinephrine and release was evoked by electrical field stimulation and kcl administration. preincubation of preparations with desipramine or pretreatment of rats with 6-hydroxydopamine significantly suppressed the uptake and evoked release of [3h]norepinephrine; electrical f ... | 1991 | 1847118 |
| mucosal ornithine decarboxylase, polyamines, and hyperplasia in infected intestine. | ornithine decarboxylase (odc), through the regulation of polyamine biosynthesis, influences normal mucosal growth and cell proliferation. the purpose of our study was to determine whether mucosal odc activity and polyamines play a role in the dramatic increase in mucosal mass and crypt elongation associated with parasite-induced inflammation in the small intestine. rats were inoculated orally with l1 larvae of the parasitic nematode trichinella spiralis and killed at different times during the e ... | 1991 | 1846274 |
| signs, larval burdens, and serological responses of dogs experimentally infected with trichinella spiralis owen, 1835. | the effects of infections of trichinella spiralis on 10 specific-pathogen-free beagles were examined. eight puppies received either 100, 500, 1,000, or 5,000 larvae, and 2 adult dogs received 1,000 larvae. blood was drawn every 4 days, beginning 5 days before infection, for the determination of relative eosinophil numbers. creatine kinase levels were monitored before infection, two weeks after infection, and one month after infection. the dogs were euthanized 1 month postinfection, and larvae we ... | 1991 | 1839680 |