Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| synthesis and in vitro anti-protozoal activity of a series of benzotropolone derivatives incorporating endocyclic hydrazines. | the preparation and evaluation as potential anti-protozoal agents of molecules bearing an endocyclic hydrazine moiety is presented. the synthetic route to this new series of compounds is straightforward, involving a hetero diels-alder reaction between different benzotropolone esters and diethyl azodicarboxylate (dead). while they show limited or no in vitro activity against leishmania donovani, plasmodium falciparum and trypanosoma brucei rhodesiense, several of the compounds have activities aga ... | 2003 | 14642327 |
| toxicity and antileishmanial activity of a new stable lipid suspension of amphotericin b. | the aim of the present study was to evaluate the toxicity and the activity of a new lipid complex formulation of amphotericin b (amb) (lc-amb; dimyristoyl phosphatidylcholine, dimyristoyl phosphatidylglycerol, and amb) that can be produced by a simple process. like other lipid formulations, this new complex reduced both the hemolytic activity of amb (the concentration causing 50% hemolysis of human erythrocytes, >100 microg/ml) and its toxicity toward murine peritoneal macrophages (50% inhibitor ... | 2003 | 14638481 |
| mechanism of impairment of cytochrome p450-dependent metabolism in hamster liver during leishmaniasis. | the mechanism of impairment of cytochrome p450 (p450)-dependent metabolism in hamster liver during leishmaniasis is reported. a significant decrease in the level of p450 was observed on the 20th day of infection when the parasite load in the liver was maximum. the decrease in p450 level was accompanied by a significant increase in the level of marker enzymes of liver and degeneration of liver tissue. the impairment was isozyme-specific and concomitant with the induction of nitric oxide synthase. ... | 2003 | 14630021 |
| effects of topoisomerases inhibitors protoberberine on leishmania donovani growth, macrophage function, and infection. | dna topoisomerases play a pivotal role in the regulation of cell division. inhibition of leishmania spp. topoisomerases represents an alternative to control parasite growth. cancer research led to the development of several potent topoisomerase inhibitors such as topoisomerase i, topoisomerase ii, or both (monobenzimidazole, terbenzimidazole, and protoberberine alkaloid-related compounds) that are effective antitumor agents. in the present study, we evaluated the efficacy of these compounds agai ... | 2003 | 14627155 |
| a novel extracellular calcium-dependent cysteine proteinase from crithidia deanei. | an extracellular cysteine proteinase from an aposymbiotic strain of crithidia deanei was purified 39-fold by a combination of anion-exchange and gel filtration chromatographies. the native molecular mass of this proteinase was estimated to be 225 kda by gel filtration chromatography and it migrates in sds-page as a single band of 80 kda. the optimal enzymatic activity on gelatin was found to occur in the presence of calcium at a neutral ph and at 28 degrees c. the enzyme was completely blocked b ... | 2003 | 14622969 |
| molecular and functional characterization of adenylate kinase 2 gene from leishmania donovani. | atp-regenerating enzymes may have an important role in maintaining atp levels in mitochondria-like kinetoplast organelle and glycosomes in parasitic protozoa. adenylate kinase (ak) (atp:amp phosphotransferase) catalyses the reversible transfer of the gamma-phosphate group from atp to amp, releasing two molecules of adp. this study describes cloning and functional characterization of the gene encoding ak2 from a genomic library of leishmania donovani and also its expression in leishmania promasti ... | 2003 | 14622299 |
| orally effective drugs for kala-azar (visceral leishmaniasis): focus on miltefosine and sitamaquine. | currently there are no effective orally administered drugs or visceral leishmaniasis or kala-azar, a parasitic disease affecting about 0.5 million people a year, majority of whom are in india and adjacent areas of nepal. symptoms of affected patients are fever, cachexia, hepatosplenomegaly and pancytopenia. the disease is usually fatal, if left untreated. traditionally kala-azar is treated with four weeks of injections of sodium stibogluconate, a pentavalent antimonial. however, this treatment h ... | 2003 | 14621038 |
| breakthrough in the management of visceral leishmaniasis. | 2003 | 14621030 | |
| immune complex antigens as a tool in serodiagnosis of kala-azar. | the 63 kda surface antigen of leishmania promastigotes is one of the most important virulent factors in establishing the host parasite relationship. this glycoprotein is revealed by surface iodination study as well as by metabolic labeling and immunoblot methods. in search of this specific antigen for serodiagnosis, immune complexes (ics) were isolated from kala-azar patient sera and analysed by sds-page and western immunoblotting. the immunoblot of kala-azar ic with patient sera, anti-promastig ... | 2003 | 14619969 |
| heat-induced reformulation of amphotericin b-deoxycholate favours drug uptake by the macrophage-like cell line j774. | heat treatment of deoxycholate-amphotericin b (amb-doc) leads to a therapeutically interesting supramolecular rearrangement (h-amb-doc); this reformulation improves the therapeutic index of amb-doc by reducing amphotericin b (amb) toxicity in mammalian cell lines from 3- to 10-fold. its activity in experimentally induced fungal infection in mice remains unchanged compared with amb-doc, whereas its activity is 2.5 times higher in leishmania donovani-infected mice. this work investigates the in vi ... | 2003 | 14613963 |
| detection of antileishmanial igg antibodies and leishmania-specific antigens in sera from yemeni patients with visceral leishmaniasis. | as part of an attempt to develop an effective method for the serodiagnosis of visceral leishmaniasis (vl), the sera from 43 yemeni cases of the disease were screened both for antileishmanial igg antibodies and for leishmania-specific antigens. indirect elisa and capture elisa were used to test each serum for the antibodies and antigens, respectively. sera from patients with diseases other than leishmaniasis (29 for the antibody-detection assays and 42 for the antigen-detection) and from apparent ... | 2003 | 14613628 |
| screening of plant extracts for antiprotozoal and cytotoxic activities. | methanolic and aqueous extracts derived from 43 plant species, selected either from ethnobotanical or chemotaxonomical data, were screened for their antiprotozoal activity against leishmania donovani and trypanosoma brucei brucei. the cytotoxic activity against kb cells was also determined. eight extracts had ic50 values of less than 10 microg/ml against leishmania donovani. the most active was triclisia patens with an ic50 value of 1.5 microg/ml against leishmania donovani. annona purpurea and ... | 2003 | 14611881 |
| real-time pcr assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. | to evaluate the usefulness of a real-time pcr for leishmania dna in the diagnosis and follow-up of patients with human immunodeficiency virus type 1 (hiv-1) and leishmania coinfection, leishmania dna levels were measured in whole peripheral blood from 25 hiv-infected patients with clinical features suggestive of visceral leishmaniasis. leishmania dna was detected in 10 of 25 patients with microscopically confirmed visceral leishmaniasis and in none of those without this disease. following treatm ... | 2003 | 14605142 |
| parasite detection in patients with post kala-azar dermal leishmaniasis in india: a comparison between molecular and immunological methods. | to evaluate the sensitivity and specificity of serological, immunohistochemical, and molecular methods in the diagnosis of post kala-azar dermal leishmaniasis (pkdl). | 2003 | 14600129 |
| differential induction of tnfalpha and il-18 in human peripheral blood mononuclear cells infected with leishmania major or leishmania donovani. | several cytokines are involved in the host response to leishmania. however, the role played by cytokines during infection with different species of leishmania is not univocal. in this work, the production of tumor necrosis factor alpha (tnfalpha) and interleukin 18 (il-18) during interaction of human phagocytes with leishmania major or l. donovani was comparatively investigated. peripheral blood mononuclear cells (pbmc) and monocytes from healthy donors were used. the release of tnfalpha and il- ... | 2003 | 14596352 |
| rab5-mediated endosome-endosome fusion regulates hemoglobin endocytosis in leishmania donovani. | to understand the trafficking of endocytosed hemoglobin (hb) in leishmania, we investigated the characteristics of in vitro fusion between endosomes containing biotinylated hb (bhb) and avidin-horseradish peroxidase (ahrp). we showed that early endosome fusion in leishmania is temperature and cytosol dependent and is inhibited by atp depletion, atpgammas, gtpgammas and n-ethylmaleimide treatment. the rab5 homolog from leishmania donovani, ldrab5, was cloned and expressed. our results showed that ... | 2003 | 14592970 |
| proteasomal degradation of a trypanosomal ornithine decarboxylase. | mammalian ornithine decarboxylase (odc), which catalyses the first step in polyamine biosynthesis, has a very fast turnover. it is degraded by the 26s proteasome in an ubiquitin-independent process and the degradation is stimulated by polyamines in a feedback control of the enzyme. interestingly, there is a major difference in the metabolic stability between odcs from various trypanosomatids. trypanosoma brucei and leishmania donovani both contain stable odcs, whereas crithidia fasciculata has a ... | 2003 | 14586177 |
| immunotherapy against murine experimental visceral leishmaniasis with the fml-vaccine. | the fucose mannose ligand (leishmania donovani fml)-saponin vaccine has earlier shown its immunoprophylactic potential against visceral leishmaniasis in the cb hamster (87.7% of parasite load reduction), balb/c (84.4%) and swiss albino mouse (85-93%) models. in this investigation its specific immunotherapeutic efficacy against l. donovani infection in balb/c mice was studied. the effects of vaccine treatment on the humoral response, delayed type of hypersensitivity to promastigote lysate (dth), ... | 2003 | 14585674 |
| modulation of t-cell costimulation as immunotherapy or immunochemotherapy in experimental visceral leishmaniasis. | cd40 ligand (cd40l)-deficient c57bl/6 mice failed to control intracellular leishmania donovani visceral infection, indicating that acquired resistance involves cd40-cd40l signaling and costimulation. conversely, in wild-type c57bl/6 and balb/c mice with established visceral infection, injection of agonist anti-cd40 monoclonal antibody (mab) induced killing of approximately 60% of parasites within liver macrophages, stimulated gamma interferon (ifn-gamma) secretion, and enhanced mononuclear cell ... | 2003 | 14573667 |
| synthesis and evaluation of novel 7-trifluoromethyl-4-(4-substituted anilino)quinolines as antiparasitic and antineoplastic agents. | several novel derivatives bearing the 7-trifluoromethyl-4-(4-substituted anilino)-quinoline skeleton were synthesized and evaluated for their in vitro activity against the blood streaming form of the parasites trypanosoma brucei rhodesiense, the trypomastigotes of trypanosoma cruzi cultured in rat skeletal myoblasts, the amastigotes form of leishmania donovani, plasmodium falciparum (k1 strain) infected erythrocyte suspension, as well as their toxicity towards rat skeletal l-6 cells. in addition ... | 2003 | 14558440 |
| 2,4-diaminopyrimidines as inhibitors of leishmanial and trypanosomal dihydrofolate reductase. | this paper describes the synthesis of 4'-substituted and 3',4'-disubstituted 5-benzyl-2,4-diaminopyrimidines as selective inhibitors of leishmanial and trypanosomal dihydrofolate reductase. compounds were then assayed against the recombinant parasite and human enzymes. some of the compounds showed good activity. they were also tested against the intact parasites using in vitro assays. good activity was found against trypanosoma cruzi, moderate activity against trypanosoma brucei and leishmania d ... | 2003 | 14556785 |
| visceral leishmaniasis in eastern sudan: parasite identification in humans and dogs; host-parasite relationships. | in 1996, an epidemic outbreak of visceral leishmaniasis (vl) started in barbar el fugara, a village in gedarif state (eastern sudan). from 1997 to 2000, regular epidemiological studies were carried out in the human population, as well as in mammals and sand flies. in symptomatic patients, 46/69 lymph node, 6/20 post kala-azar dermal leishmaniasis (pkdl) and 1/4 cutaneous cultures in nnn medium were positive. in 69 dogs, 23/79 lymph node cultures were positive. in other mammals (47 rodents, five ... | 2003 | 14554251 |
| isolation, characterization and expression of a cyclin from leishmania donovani. | we have cloned and sequenced a dna fragment (approximately 1 kb) containing a complete open reading frame from a cdna library of leishmania donovani promastigotes. the alignment of the derived polypeptide sequence and the modeling studies revealed that the protein is highly homologous to the mammalian cyclins having conserved cyclin box and substrate-docking motif. northern blot analysis of the rna isolated from synchronized l. donovani promastigotes showed periodic expression of the message wit ... | 2003 | 14553924 |
| an in vitro system for developmental and genetic studies of leishmania donovani phosphoglycans. | glycoconjugates have been shown to play important roles in leishmania development. however, the ability to study these molecules and other processes would benefit greatly from improved methods for genetic manipulation and analysis of the amastigote stage. this is especially challenging for l. donovani, the agent of the most severe form of leishmaniasis, which can rapidly lose virulence during in vitro culture. here we report on a clonal subline of an l. donovani 1s2d (ldbob or ldb), which differ ... | 2003 | 14550894 |
| drug targeting by macromolecules without recognition unit? | his review will summarize available information on the ability of macromolecular conjugates containing no specific recognition motifs to deliver anthracyclines (daunomycin, adriamycin) or methotrexate to target cells such as tumour cells or macrophages. conjugates with natural (proteins, dna, carbohydrates) and synthetic macromolecules (linear and branched chain poly-alpha-amino acids, non-biodegradable divema, hpma etc.) will be reviewed. experimental data from several laboratories indicate tha ... | 2003 | 14523942 |
| slc11a1 (formerly nramp1) and susceptibility to visceral leishmaniasis in the sudan. | genetic susceptibility to visceral leishmaniasis (vl) is indicated by differences in incidence and clinical phenotypes between ethnic groups in sudan. in mice, innate susceptibility to leishmania donovani, the etiological agent of vl, is controlled by slc11a1 (formerly nramp1). we therefore examined polymorphisms at slc11a1 in 59 multicase families of vl from the high-incidence masalit tribe in sudan. multipoint nonparametric analysis in allegro shows a significant linkage across slc11a1 (zlr sc ... | 2004 | 14523377 |
| images in clinical medicine. kala-azar. | 2003 | 14523156 | |
| characterisation of leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine). | leishmania donovani promastigote lines resistant to hexadecylphosphocholine (hepc, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 microm were developed in vitro by continuous step-wise drug pressure. the 40 microm line was 15 times more resistant to hepc than the wild-type clone and showed cross-resistance to the ether lipid et-18-och3 (edelfosine) but not to the standard anti-leishmanial drugs. resistance was stable up to 12 weeks in drug-free culture medium. no amplification of specific genes, ... | 2003 | 14522101 |
| functional cloning of the miltefosine transporter. a novel p-type phospholipid translocase from leishmania involved in drug resistance. | the antitumor drug miltefosine (hexadecylphosphocholine, mil) has recently been approved as the first oral agent for the treatment of visceral leishmaniasis. little is known about the mechanisms of action and uptake of mil in either parasites or tumor cell lines. we have cloned a putative mil transporter (ldmt) by functional rescue, using a leishmania donovani-resistant line defective in the inward-directed translocation of both mil and glycerophospholipids. ldmt is a novel p-type atpase belongi ... | 2003 | 14514670 |
| ancistrotanzanine a, the first 5,3'-coupled naphthylisoquinoline alkaloid, and two further, 5,8'-linked related compounds from the newly described species ancistrocladus tanzaniensis. | the first phytochemical investigation of the recently discovered east african liana ancistrocladus tanzaniensis is described, resulting in the isolation and structural elucidation of two new naphthylisoquinoline alkaloids, ancistrotanzanines a (5) and b (6), and the known compound ancistrotectoriline a (7). ancistrotazanine a (5) represents a hitherto unprecedented 5,3'-coupling type between the naphthalene and isoquinoline portions, while 6 and 7 are 5,8'-coupled. the structures of the compound ... | 2003 | 14510589 |
| the role of interleukin-10 in susceptibility of balb/c mice to infection with leishmania mexicana and leishmania amazonensis. | recent studies have demonstrated the critical role of il-10 in susceptibility to cutaneous and visceral leishmaniasis caused by leishmania major and leishmania donovani, respectively. to determine whether il-10 also plays a similar role in the susceptibility and pathogenesis of cutaneous leishmaniasis caused by the new world species, l. mexicana and l. amazonensis, we analyzed their course of infection in il-10-deficient balb/c mice and their wild-type counterparts. although il-10-deficient mice ... | 2003 | 14500669 |
| [persistence of leishmania donovani in the body of the dog after clinical and serological cure of general leishmaniasis]. | 1962 | 14488321 | |
| [experimental leishmaniasis in the mouse (leishmania donovani and leishmania tropica)]. | 1961 | 14486196 | |
| [contribution to the study of the pathogenesis and histopathology of leishmania donovani infection in the golden hamster (cricetus auratus)]. | 1961 | 14467439 | |
| serum proteins of animals infected with leishmania donovani, with special reference to electrophoretic patterns. | 1960 | 14439190 | |
| stages in the life-cycle of leishmania donovani. | 1960 | 14435316 | |
| [behavior of leishmania donovani in various rodent species]. | 1964 | 14340332 | |
| [on the susceptibility of some xerothermophilic rodents for experimental infections with leishmania donovani (calcutta strain)]. | 1964 | 14316627 | |
| an autopsy case of mixed infection of leishmania donovani and blastomyces brasiliensis with emphasis on the histopathogenesis of reticuloendotheliosis. | 1964 | 14246014 | |
| [installation, multiplication and maintenance of a leishmania donovani strain in cell culture]. | 1964 | 14185702 | |
| the fine structure of leishmania donovani and the role of the kinetoplast in the leishmani-leptomonad transformation. | 1964 | 14184112 | |
| [experimental infection of cerdocyon thous (common fox) with leishmania donovani. preliminary note]. | 1964 | 14183231 | |
| [comments on the phylogeny and pathogenicity of leishmania donovani]. | 1964 | 14172852 | |
| preservation of leishmania donovani by low-temperature freezing. | 1964 | 14134740 | |
| free amino-acid pool of leishmania donovani. | 1963 | 14076078 | |
| action of an antifungal antibiotic nystatin on the protozoa leishmania donovani. vi. studies on the action on isolated membrane and the isolation of antibiotic-rich cell particle from l. donovani. | 1963 | 14076073 | |
| [contribution to the study of the culture of leishmania donovani in chick embryos]. | 1963 | 14075064 | |
| action of an antifungal antibiotic, nystatin, on the protozoa, leishmania donovani. v: studies on the absorption of nystatin by l. donovani. | 1963 | 14057655 | |
| [contribution to the study of the culture of leishmania donovani in chick embryos]. | 1963 | 14053496 | |
| a new experimental host of leishmania donovani. | 1963 | 14053104 | |
| resistance to the drug propamidine in leishmania donovani. | 1963 | 14050730 | |
| [on the experimental infection of "phlebotomus longipalpis" by "leishmania donovani"]. | 1961 | 14039201 | |
| action of an antifungal antibiotic, nystatin, on the protozoa leishmania donovani. iv. studies on the cytological and cytochemical changes. | 1963 | 13947517 | |
| action of an antifungal antibiotic, nystatin, on the protozoa leishmania donovani. iii. studies on the lysis of the cells of l. donovani. | 1963 | 13947516 | |
| isolation and characterization of the membrane of leishmania donovani. | 1963 | 13947515 | |
| pathogenicity of leishmania donovani in man. | 1962 | 13910616 | |
| leishmanicidal activity of nystatin, a polyene antifungal antibiotic. ii. isolation of bound nystatin from cells of leishmania donovani and its clinical application. | 1962 | 13898230 | |
| leishmanicidal activity of nystatin, a polyene antifungal antibiotic. i. the probable mechanism of action of nystatin on leishmania donovani. | 1962 | 13898229 | |
| action of an antifungal antibiotic, nystatin, on the protozoa leishmania donovani. ii. studies on the release of intracellular constituents. | 1961 | 13898228 | |
| action of an antifungal antibiotic, nystatin, on the protozoa leishmania donovani. i. studies on the metabolism of leishmania donovani. | 1961 | 13898227 | |
| [reservoirs of leishmania donovani in brazil]. | 1961 | 13884628 | |
| cytology of the flagellate form of leishmania donovani with consideration of the evidence for abnormal nuclear division. | 1962 | 13877899 | |
| mitotic cycle of the kala-azar parasite, leishmania donovani. | 1962 | 13877898 | |
| carbon dioxide assimilation by leishmania donovani. | 1960 | 13809443 | |
| [ultrastructure of leishmania donovani]. | 1960 | 13732544 | |
| treatment of leishmania donovani infections with surface-active agents. | 1960 | 13702571 | |
| structure-activity relationships of the antimalarial agent artemisinin. 8. design, synthesis, and comfa studies toward the development of artemisinin-based drugs against leishmaniasis and malaria. | artemisinin (1) and its analogues have been well studied for their antimalarial activity. here we present the antimalarial activity of some novel c-9-modified artemisinin analogues synthesized using artemisitene as the key intermediate. further, antileishmanial activity of more than 70 artemisinin derivatives against leishmania donovani promastigotes is described for the first time. a comprehensive structure-activity relationship study using comfa is discussed. these analogues exhibited leishman ... | 2003 | 13678403 |
| cultivation of leishmania donovani in human amnion epithelial cell tissue cultures: a preliminary report. | 1958 | 13585307 | |
| hexokinase activity in cell-free extracts of leishmania donovani. | 1958 | 13566200 | |
| electron microscopic investigations on the leptomonad form of leishmania donovani. | 1958 | 13524236 | |
| [evolution of kala-azar in dogs experimentally infected with leishmania donovani]. | 1957 | 13522254 | |
| transaminases of leishmania donovani, the causative organism of kala-azar. | 1957 | 13493550 | |
| [man, a possible reservoir of the virus of leishmania donovani; primary & secondary cutaneous localizations of kala-azar]. | 1957 | 13466290 | |
| cytochemical studies of mitochondria in leptomonad form of leishmania donovani, the kala-azar parasite. | 1956 | 13332233 | |
| [schizogony forms of leishmania donovani in human bone marrow]. | 1956 | 13325765 | |
| the ultrastructure of leishmania donovani. | 1956 | 13320253 | |
| [dogs naturally infected by leishmania donovani in ceará]. | 1954 | 13191740 | |
| [natural infection of phlebotomus longipalpis by leptomonas, probably leishmania donovani, in a focus of kala-azar in ceará]. | 1954 | 13191739 | |
| electrophoretic patterns of the serum proteins of chinchillas and hamsters infected with leishmania donovani. | 1954 | 13183092 | |
| [multiple reproduction of leishmania donovani laveran and mesnil 1903]. | 1954 | 13182505 | |
| [leishmania donovani laveran and mesnil 1903 also parasitic in erythrocytes]. | 1954 | 13182504 | |
| observations on the structure of leishmania donovani; the kala-azar parasite. | 1954 | 13173489 | |
| the metabolism of leishmania donovani in culture. | 1954 | 13140170 | |
| antiprotozoal activities of symmetrical bishydroxamic acids. | symmetrical bishydroxamic acids along with their sodium salts containing an alkyl spacer between two aromatic rings were synthesized, and their antiparasitic activities were evaluated. bishydroxamic acids were conveniently prepared from the alkylation of methyl 4-hydroxybenzoate with various dihalo-alkane, -alkene, and -ether followed by reaction with hydroxylamine. surprisingly, the bishydroxamic acids and their sodium salts possess strong inhibitory activities against plasmodium falciparum par ... | 2003 | 13129572 |
| the cytology of leishmania donovani (laveran & mesnil, 1903) ross, 1903. | 1953 | 13069725 | |
| the development of leishmania donovani in vitro at 37 degree c; effects of the kind of serum. | suspensions of leishmanias from the spleen of hamsters infected with leishmania donovani were placed in culture flasks and incubated at 37 degrees c. in a medium of human erythrocyte extract and human serum there appeared within a day or two aflagellate forms resembling leishmanias but larger, as well as other aflagellate forms more nearly resembling rounded leptomonads. these intermediate forms multiplied during the first 4 days of culture. they then slowly died off, despite frequent renewal of ... | 1953 | 13022872 |
| developmentally induced changes of the proteome in the protozoan parasite leishmania donovani. | in order to proceed through their life cycle, protozoan parasites of the genus leishmania cycle between sandflies and mammals. this change of environment correlates with the differentiation from the promastigote stage (insect form) to the amastigote stage (intracellular mammalian form). the molecular basis underlying this major transformation is poorly understood so far; however, heat shock protein 90 (hsp90) appears to play a pivotal role. to further elucidate this process we identified protein ... | 2003 | 12973740 |
| post-kala-azar dermal leishmaniasis (pkdl): the first case report from nepal. | post-kala-azar dermal leishmaniasis (pkdl) is condition characterized by non-ulcerative lesions of the skin caused by leishmania donovani that is usually seen after the completion of treatment of kala-azar (visceral leishmaniasis). we document the first case report of pkdl in nepal. | 2003 | 12971510 |
| abortive infection of lutzomyia longipalpis insect vectors by aflagellated ldarl-3a-q70l overexpressing leishmania amazonensis parasites. | leishmania donovani adp-ribosylation factor-like protein 3a (ldarl-3a) is a small g protein isolated from the protozoan parasite l. donovani with no defined physiological function. previously [cuvillier, a., redon, f., antoine, j.-c., chardin, p., devos, t., and merlin, g. (2000) j cell sci 113: 2065-2074] we have shown that overexpression in l. amazonensis promastigotes of the mutated protein ldarl-3a-q70l, which remains constitutively associated with gtp, leads to the disappearance of the flag ... | 2003 | 12969377 |
| bioactive alkyl phenols and embelin from oxalis erythrorhiza. | the benzoquinone embelin and four alkyl phenols were isolated from an argentinean collection of oxalis erythrorhiza. 3-heptadecyl-5-methoxy-phenol is reported for the first time. the structures were determined by spectroscopic methods. embelin presented inhibitory effect on methicillin-resistant staphylococcus aureus, escherichia coli and the dermatophytic fungi epidermophyton floccosum, microsporum canis, microsporum gypseum, trichophyton mentagrophytes and trichophyton rubrum with mics ranging ... | 2003 | 12963150 |
| multinutrient undernutrition dysregulates the resident macrophage proinflammatory cytokine network, nuclear factor-kappab activation, and nitric oxide production. | we have described previously a murine model of multinutrient undernutrition that reproduced the features of moderate human malnutrition and led to increased early dissemination of leishmania donovani. peritoneal cells from these malnourished mice produced decreased no after stimulation with ifn-gamma/lps. we hypothesized that malnutrition may cause a deficit in nf-kappab activation, a principal transcription pathway for inducible no synthase and proinflammatory cytokines. macrophages from malnou ... | 2003 | 12960263 |
| functional dissection of the c-terminal domain of type ii dna topoisomerase from the kinetoplastid hemoflagellate leishmania donovani. | the amino acid sequences of the c-terminal domain (ctd) of the type ii dna topoisomerases are divergent and species specific as compared with the highly conserved n-terminal and central domains. a set of c-terminal deletion mutants of leishmania donovani topoisomerase ii was constructed. removal of more than 178 amino acids out of 1236 amino acid residues from the c-terminus inactivates the enzyme, whereas removal of 118 amino acids or less has no apparent effect on the ability of the parasite e ... | 2003 | 12954766 |
| prophylactic potential of autoclaved leishmania donovani with bcg against experimental visceral leishmaniasis. | the prophylactic efficacy of autoclaved leishmania donovani (ald) and autoclaved l. major (alm)--a heterologous vaccine developed against cutaneous leishmaniasis (used as a reference vaccine), along with bcg--was evaluated against l. donovani in hamsters (mesocricetus auratus). animals were immunized with triple doses (21 days apart) of either ald or alm (1.0 mg) with or without bcg (0.1 mg) and challenged 21 days later with 1 x 10(6) l. donovani amastigotes intracardially. animals immunized wit ... | 2003 | 12954011 |
| case 2. cutaneous leishmaniasis, leishmania amastigotes. | 2003 | 12950363 | |
| membrane orientation of laminin binding protein. | earlier we presented several lines of evidence that a 67-kda laminin binding protein (lbp) in leishmania donovani, that is different from the putative mammalian 67-kda laminin receptor, may play an important role in the onset of leishmaniasis, as these parasites invade macrophages in various organs after migrating through the extracellular matrix. here we describe the membrane orientation of this leishmania laminin receptor. flow cytometric analysis using anti-lbp ig revealed its surface localiz ... | 2003 | 12950264 |
| naphthoquinone derivatives and lignans from the paraguayan crude drug "tayï pytá" (tabebuia heptaphylla, bignoniaceae). | the paraguayan crude drug "tayï pytá" is used to treat cancer, wounds and inflammation. it consist of the bark and trunkwood of tabebuia heptaphylla (bignoniaceae). a phytochemical study of the crude drug gave, in addition to previously described naphthoquinones and the known lignans cycloolivil and secoisolariciresinol, three new lapachenol (lapachonone)-, two naphthofuran-, a chromone and a naphthalene derivative. the structures were elucidated by means of high field nmr spectroscopy. the biol ... | 2003 | 12939034 |
| extracellular release of the surface metalloprotease, gp63, from leishmania and insect trypanosomatids. | protease activity was found in spent culture medium collected from leishmania donovani, l. mexicana, l. major, as well as the insect trypanosomatids, crithidia luciliae and leptomonas seymouri. released protease activity increased linearly over time and was correlated to promastigote density. in sds-page, zymogram gels showed that the protease's molecular weight ranged from 43-100 kda. spent culture medium proteases were blocked by the metallo-protease inhibitors, 1,10-phenanthroline and z-tyr-l ... | 2003 | 12923634 |
| selection of aptamers against kmp-11 using colloidal gold during the selex process. | selex procedure is a methodology in which single stranded oligonucleotides are selected from a wide variety of sequences based on their interaction with a target molecule. we have designed a novel selex methodology using colloidal gold to select high affinity single stranded dna aptamers against leishmania infantum kmp-11. kinetoplastid membrane protein-11 (kmp-11) is a major component of the cell membrane of kinetoplastid parasites. although its function is not known, the fact that kmp-11 is a ... | 2003 | 12901856 |
| novel mechanism of drug resistance in kala azar field isolates. | clinical resistance to pentavalent antimonial drugs in the form of sodium antimony gluconate (sag) has become a major problem in the treatment of kala azar (visceral leishmaniasis) in india. the mechanism of resistance is unclear in these clinical isolates, although work has been conducted with leishmania species mutants selected in vitro by stepwise increase of drug concentration, using antimony-related metal arsenic and, more recently, sag. in the present study, we investigated the molecular a ... | 2003 | 12898450 |
| immunochromatographic (rk39) strip test in the diagnosis of visceral leishmaniasis in bangladesh. | immunochromatographic strip test (ict strip test) for the diagnosis of visceral leishmaniasis was evaluated in this study in the context of a case-control study. a total sixty consecutive cases of kala-azar admitted in all four medicine units of mymensingh medical college hospital during the period of may 2002 to february 2003 was included here. parasitological confirmation was done by demonstration of leishmania donovani bodies in bone marrow or splenic aspiration in all cases. a total 120 cont ... | 2003 | 12894040 |