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a highly unusual combination of pulmonary pathogens in an hiv infected patient.we report on a case with an unusual combination of pulmonary pathogens including pneumocystis carinii, rhodococcus equi and toxoplasma gondii in an hiv infected patient. the multiple microbiological procedures required to ascertain the etiological diagnoses of pneumonitis in hiv-seropositive subjects are discussed.19948036478
rhodococcus equi: first case in a heart transplant recipient.rhodococcus equi is a gram-positive diphtheroid that occasionally affects immunocompromised patients, usually causing a chronic respiratory infection with cavitating pulmonary opacities on chest radiograph that resemble mycobacterial or fungal disease. etiologic diagnosis presents a number of pitfalls, because rhodococcus equi isolates mimic many of the characteristics of other microorganisms more familiar to the laboratory staff. the treatment of choice for this disease has not yet been establi ...19948031817
purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in rhodococcus ruber.the n-terminal amino acid sequence of the polyhydroxyalkanoic acid (pha) granule-associated m(r)-15,500 protein of rhodococcus ruber (the ga14 protein) was analyzed. the sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated m(r) of 14,175 which was recently localized downstream of the pha synthase gene (u. pieper and a. steinbüchel, fems microbiol. lett. 96:73-80, 1992). a recombinant strain of escherichia coli xl1-b ...19948021220
metabolism of styrene by rhodococcus rhodochrous ncimb 13259.rhodococcus rhodochrous ncimb 13259 grows on styrene, toluene, ethylbenzene, and benzene as sole carbon sources. simultaneous induction tests with cells grown on styrene or toluene showed high rates of oxygen consumption with toluene cis-glycol and 3-methylcatechol, suggesting the involvement of a cis-glycol pathway. 3-vinylcatechol accumulated when intact cells were incubated with styrene in the presence of 3-fluorocatechol to inhibit catechol dioxygenase activity. experiments with 18o2 showed ...19948017910
fatal rhodococcus equi infection in an angora goat. 19948011764
molecular cloning and nucleotide sequence of the gene coding photosensitive nitrile hydratase. 19948010666
[emergent pathologies: rhodococcus equi infection in the acquired immunodeficiency syndrome].rhodococcus equi is an intracellular facultative, gram-positive, partially acid-fast, cocco-bacillary microorganism of increasing importance as a pathogen in severely immunocompromised hiv-positive patients. rhodococcus equi may cause invasive pulmonary infection, bacteremia and disseminated infection. rhodococcus equi is easily cultured from blood or sputum on standard media, but is frequently regarded as a contaminant. delay in establishing a definite diagnosis may result in a poor outcome in ...19948003385
rapid degradation of the triazinone herbicide metamitron by a rhodococcus sp. isolated from treated soil.seven bacterial isolates which degraded the herbicide metamitron (3-methyl-4-amino-6-phenyl-1,2,4-triazin-5-one) were obtained from field-enhanced soil by liquid enrichment culture. all isolates appeared to be identical and a representative, 0246b, was identified as a rhodococcus sp. by cell wall and fatty acid analyses. this isolate degraded metamitron as the sole source of carbon within 24 h at 25 degrees c and this is the first report of a bacterium capable of growing with metamitron as the s ...19948002472
stereoselective epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile.the chiral intermediate (3s,4r)-trans-3,4-dihydro-3,4-dihydroxy-2,2-dimethyl- 2h-1-benzopyran-6-carbonitrile [(+)-trans diol 3] was made by the stereoselective microbial epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile 1. this compound is a potential intermediate for the total synthesis of potassium-channel openers. several microbial cultures were found which catalyzed the transformation of 1 to the corresponding (3s,4s)-epoxide 2 and (+)-trans diol 3. the two best cultures, corynebact ...19948000876
enzymatic asymmetric synthesis of alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols by arylalkyl acylamidases.with the novel microbial enzyme, 'arylalkyl acylamidase', optically active alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols have been obtained through enantioselective hydrolysis of their racemic amides and esters. (s)-enantiomers of 1-methylbenzylamine, 1-methyl-3-phenylpropylamine and 1-methyl-3-phenylpropanol of high optical purity (> 94% e.e.) were synthesized with the cells of nocardia erythropolis iam 1440 or cellulomonas fimi aku 671. (r)-enantiomer of 1-methyl-3-phenylprop ...19948000864
a kinetic study and application of a novel carbonyl reductase isolated from rhodococcus erythropolis.the newly described carbonyl reductase from rhodococcus erythropolis (recr) accepts a broad range of substrates. based on the kinetic constants of a variety of methyl and ethyl ketones a hypothetical model of the substrate-binding site is proposed. whether a substrate of interest may be reduced by the recr can be predicted from this model together with the kinetic data. a study of initial velocities and product inhibition is presented, which shows that the kinetics of the recr follow a theorell- ...19948000863
permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes.the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ...19948000549
evaluation of a monoclonal antibody-based colony blot test for rapid identification of virulent rhodococcus equi.we recently generated a monoclonal antibody immunoglobulin g1 (mab 10g5), which can recognize 15- to 17-kda antigens, virulence-associated antigens of rhodococcus equi, and developed a colony blot enzyme-linked immunosorbent assay with mab 10g5 for the rapid identification of virulent r. equi. in this epidemiologic study, we evaluated the results of the colony blot test in the identification of virulent isolates of r. equi from feces of horses and soil and compared them with those from a convent ...19947999891
haemolytic and phospholipase c (plc) activities of rhodococcus equi.rhodococcus equi, an intracellular organism causing pneumonia and lung abscesses in foals, is generally thought to be non-haemolytic. in the present study, however, 13 of 14 representative isolates were found to be haemolytic when tested on agar media containing washed red blood cells rather than whole blood. red cells of rabbits, dogs, horses and man were more sensitive to lysis than were those of ruminants. two new enzymatic activities of the species were defined: a lecithinase and a phosphati ...19947989259
review of 40 cases of lung abscesses in foals and adult horses.a retrospective study of 40 horses with primary lung abscesses without pleuropneumonia showed that lung abscesses occurred predominantly in young horses, usually in foals aged < or = 6 months. hyperfibrinogenaemia was present in all but 2 horses, and other common clinical and haematological findings were hyperthermia, tachycardia, polypnoea, and neutrophilic leucocytosis. the most common bacterial species isolated from transtracheal aspirates were streptococcus zooepidemicus (20/34) and rhodococ ...19947988535
rhodococcus equi pneumonia in an hiv-infected patient.we report a case of a 46-year-old hiv-infected patient suffering rhodococcus equi pneumonia and septicaemia. after the failure of an initial antibiotic treatment, the upper lobe of the right lung was resected due to a cavitating pneumonia. after that the patient stabilized for a period of 7 months by administration of a resistogram-adapted combination of tetracycline, erythromycin and clindamycin. due to the patient's decision, antibiotic treatment was stopped when a cytomegalovirus retinitis wa ...19947987063
brain abscess due to gordona terrae in an immunocompromised child: case report and review of infections caused by g. terrae.a brain abscess complicated antineoplastic chemotherapy for a primary cerebral rhabdoid tumor in an immunocompromised boy. culture of purulent exudate obtained by surgical puncture of an intracranial hematoma yielded a gram-positive microorganism initially identified as a rhodococcus species by conventional biochemical analysis; however, the isolate was subsequently identified as gordona terrae by ribosomal dna analysis. to our knowledge, this is the third case of human infection caused by g. te ...19947986897
identification of a heroin esterase in rhodococcus sp. strain h1.a strain of a rhodococcus sp. (termed h1) capable of utilizing heroin as its sole carbon and energy source was isolated by selective enrichment. an inducible heroin esterase was partially purified and shown to catalyze the hydrolysis of both of the acetylester groups of heroin. the enzyme displays optimum activity at ph 8.5 and appears to be a trimer of identical subunits with an m(r) or 39,000 and a native m(r) of 120,000.19947986057
transfer of rhodococcus aichiensis tsukamura 1982 and nocardia amarae lechevalier and lechevalier 1974 to the genus gordona as gordona aichiensis comb. nov. and gordona amarae comb. nov.we investigated the taxonomic status of rhodococcus aichiensis dsm 43978t (t = type strain) and nocardia amarae dsm 43392t by using both chemotaxonomic and genetic methods. the occurrence of mk-9(h2) as the predominant menaquinone, the presence of relatively long-chain mycolic acids (48 to 62 carbon atoms), and the phylogenetic position as determined by a 16s ribosomal dna sequence analysis provide strong evidence that both r. aichiensis and n. amarae should be transferred to the genus gordona a ...19947981103
[serodiagnosis of caseous lymphadenitis (pseudotuberculosis) in sheep].random postmortal isolation of corynebacterium pseudotuberculosis from the sheep used in a research project and the consequently determined serological positivity of these animals indicated the serological examination of the flock from which the sheep came. no symptoms of caseous lymphadenitis were ever observed in the flock in question and no data on the occurrence of this disease were available in the flock history. the sera of 228 head of sheep were used for examinations, while this number of ...19947975051
evaluation of api coryne system for identifying coryneform bacteria.to identify rapidly and accurately coryneform bacteria, using a commercial strip system.19947962633
characterization of the desulfurization genes from rhodococcus sp. strain igts8.rhodococcus sp. strain igts8 possesses an enzymatic pathway that can remove covalently bound sulfur from dibenzothiophene (dbt) without breaking carbon-carbon bonds. the dna sequence of a 4.0-kb bstbi-bsiwi fragment that carries the genes for this pathway was determined. frameshift and deletion mutations established that three open reading frames were required for dbt desulfurization, and the genes were designated soxabc (for sulfur oxidation). each sox gene was subcloned independently and expre ...19947961424
bacterial oxidation of propane.the bacterial metabolism of propane and the pathway(s) involved are poorly understood, as the relative importance of terminal versus subterminal oxidation of propane, via propan-1-ol and propan-2-ol, respectively, is still unclear. in the case of bacteria, the ability to oxidize propane appears to be confined mainly to the gram-positive corynebacterium - nocardia - mycobacterium - rhodococcus complex. studies on propane oxidation have been hampered by a lack of firm enzymological data; for examp ...19947958761
the enzymes of beta-oxidation in rhodococcus ruber. 19947958285
purification and characterization of an amidase from an acrylamide-degrading rhodococcus sp.a constitutively expressed aliphatic amidase from a rhodococcus sp. catalyzing acrylamide deamination was purified to electrophoretic homogeneity. the molecular weight of the native enzyme was estimated to be 360,000. upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified preparation yielded a homogeneous protein band having an apparent molecular weight of about 44,500. the amidase had ph and temperature optima of 8.5 and 40 degrees c, respectively, and its isoelectric poin ...19947944367
pulmonary malacoplakia associated with rhodococcus equi infection in a patient with acquired immunodeficiency syndrome.pulmonary malacoplakia associated with rhodococcus equi, an opportunistic gram-positive coccobacillus, is unusual. although this patient is the ninth reported to have pulmonary malacoplakia, he is only the third patient with the acquired immunodeficiency syndrome who has been reported to have pulmonary malacoplakia associated with r equi infection. the patient was a 49-year-old man infected with the human immunodeficiency virus who on initial examination was found to have cavitary pneumonia in t ...19947942603
[new macrolides].the macrolides are still widely used in general practice and the new 14- or 15 membered-ring compounds-roxithromycin, clarithromycin, dirithromycin, azithromycin -may offer a new development. their antibacterial activity is similar to erythromycin but azithromycin is more active against gram negative strams. roxythromycin exhibits high serum concentrations; azithromycin and dirithromycin present low serum concentrations but prolonged half-lives allowing a reduction of the duration of treatment p ...19947939238
phosphorylative inactivation of rifampicin by nocardia otitidiscaviarum.rifampicin was inactivated by nocardia otitidiscaviarum to yield two products designated rip-3 and rip-4. the full structures of rip-3 and rip-4 were determined to be 21-(o-phosphoryl) rifampicin and 3-formyl-21-(o-phosphoryl) rifamycin sv, respectively, but neither product had any antimicrobial activity.19947928806
survival and replication of rhodococcus equi in macrophages.rhodococcus equi is a facultative intracellular bacterium of macrophages that can cause serious pneumonia in both young horses and immunocompromised people. essential to understanding rhodococcus pathogenesis is a quantitative documentation of the intracellular events that follow macrophage phagocytosis of the organism. by using a bacterial immunofluorescence staining assay, we verified the intracellular survival and replicative potential of r. equi in both murine peritoneal macrophages and equi ...19947927672
tamponade due to rhodococcus equi in acquired immunodeficiency syndrome.infection with rhodococcus equi has been described as a cause of cavitary pneumonia in aids patients. we report such a case, complicated by bacteremia, tamponade, and possible brain and liver abscesses. medical treatment was successful with pericardiocentesis and antibiotherapy with teicoplanin, gentamicin, clarithromycin, rifampicin, supplemented by empirical treatment of cerebral toxoplasmosis. antibiotherapy was terminated after 6 months, without relapse 2 months later.19947924515
detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens.the polymerase chain reaction (pcr) and automated dna sequencing were used to detect a genetic locus, rpob, associated with rifampin resistance in mycobacterium tuberculosis (tb) in clinical isolates and directly in clinical specimens. primers derived from the sequence of a tb rpob gene fragment were used to amplify dna from bacterial and mycobacterial isolates. an rpob-specific pcr product was obtained for five of five tb, seven of eight other mycobacterial species, nocardia sp., corynebacteriu ...19947924218
bacteria and pesticides: a new aspect of interaction--involvement of a new biofactor.positively charged hydrophobic pesticides of the dipyridyl family [diquat, paraquat, benzylviologen (bv++), etc.] were shown to provoke accumulation of 2-methylbutane-1,2,3,4-tetraol-2,4- cyclopyrophosphate in the cells corynebacterium (brevibacterium) ammoniagenes while neutral dipyridyls were not. hydrophobicity was also an important factor in this phenomenon. of the other pesticides tested, only linuron was effective. bv++ also induced biosynthesis of the compound in rhodococcus rhodochrous, ...19947916959
amidase coupled with low-molecular-mass nitrile hydratase from rhodococcus rhodochrous j1. sequencing and expression of the gene and purification and characterization of the gene product.the cloned 9.4-kb insert of plasmid pnhj20l containing low-molecular-mass nitrile hydratase (l-nhase) gene from rhodococcus rhodochrous j1 [kobayashi, m. et al. (1991) biochim. biophys. acta 1129, 23-33] was digested with various restriction enzymes, and the trimmed fragments were inserted into puc18 or puc19. a 1.96-kb ecori-sphi region located 1.9-kb downstream of the l-nhase gene was found to be essential for the expression of amidase activity in escherichia coli; the gene arrangement of the ...19937916690
[nosocomial infections: new agents, incidence, prevention].nosocomial infection is one of the major health problems confronting clinicians in intensive care units. incidence has been estimated to vary from 5 to 10%, leading to death in one of every 5000 persons hospitalized. this situation is not however static since hospital epidemics and nosocomial infections evolve as a consequence of advances in antibiotic drug therapy and surgical and intensive care techniques. microbial investigations themselves are of major importance as the collection of epidemi ...19957899352
[distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms].the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ...19947898396
isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker.the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ...19957896070
brevibacterium linens pbl33 and rhodococcus rhodochrous prc1 cryptic plasmids replicate in rhodococcus sp. r312 (formerly brevibacterium sp. r312).the replication of two cryptic plasmids from brevibacterium linens atcc 9174 (pbl33) and rhodococcus rhodochrous atcc 4276 (prc1) was investigated in rhodococcus sp. r312 (formerly brevibacterium sp. r312). the recombinant plasmids psp33 (pbl33 derivative) and pspc1 (prc1 derivative) were found to be suitable for establishing new host-vector systems for rhodococcus sp. r312. they all carry the tn903 neomycin-resistance-encoding gene (aphi).19957867954
o-, m- and p-hydroxybenzoate degradative pathways in rhodococcus erythropolis.rhodococcus erythropolis strain s1 uses the gentisate pathway to metabolize salicylate and m-hydroxybenzoate and the protocatechuate pathway to degrade p-hydroxybenzoate. m-hydroxybenzoate 6-hydroxylase was induced by growth on m-hydroxybenzoate or gentisate, and salicylate 5-hydroxylase only by growth on salicylate. p-hydroxybenzoate 3-hydroxylase could be induced only by growth on p-hydroxybenzoate. m-hydroxybenzoate or p-hydroxybenzoate could repress the induction of salicylate 5-hydroxylase. ...19957867918
enzyme-catalysed enantioselective hydrolysis of racemic naproxen nitrile.the bacterial strain rhodococcus butanica (atcc 21197), which exhibits nitrilase and nitrile hydratase/amidase activities, catalyses the enantioselective hydrolysis of racemic naproxen nitrile (r/s)-1 to furnish a moderate enantiomeric excess of (s)-naproxen (s)-3. racemic naproxen amide (r/s)-2 is not a good substrate for this strain. resting cells of the newly selected bacterial strain rhodococcus sp. c3ii catalyse the enantioselective hydrolyses of racemic naproxen nitrile (r/s)-1 and naproxe ...19947858980
dietzia, a new genus including dietzia maris comb. nov., formerly rhodococcus maris.sequencing of the 16s ribosomal dnas (rdna) of two strains of rhodococcus maris was performed to determine the relationship of this species to other mycolic acid-containing actinomycetes. for this purpose we also determined the 16s rdna sequences for the type species of the genus rhodococcus, rhodococcus rhodochrous, and for mycobacterium chlorophenolicum (formerly rhodococcus chlorophenolicus), rhodococcus erythropolis, gordona bronchialis, and gordona terrae, for which only partial sequence da ...19957857805
amino acid sequence analysis of ribosomal protein at-l30 from members of the family pseudonocardiaceae.the phylogenetic relationships of the genera belonging to the family pseudonocardiaceae were examined by a novel approach, amino acid sequencing of ribosomal at-l30 proteins. the results of partial amino acid sequencing of at-l30 preparations revealed that the members of the family pseudonocardiaceae are divided into four clusters; the first cluster contains the genus actinopolyspora, the second cluster contains the genus saccharopolyspora, the third cluster contains the genus amycolatopsis, and ...19957857791
routine identification of mycobacterium tuberculosis complex isolates by automated hybridization.methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. we describe here a 2-h identification system for mycobacterial isolates belonging to the mycobacterium tuberculosis complex using a dna probe. after 30 min of hands-off sample preparation, the 1.5-h hybridization test is totally automated in the newly developed vidas system (biomérieux, marcyl'etoile, france), which performs solid-phase specif ...19947852560
rhodococcus equi: fatal pneumonia in a patient without aids.rhodococcus equi infections are being recognized with greater frequency in immunocompromised patients and the population infected with the human immunodeficiency virus (hiv). r. equi usually causes a progressive pneumonia that may or may not cavitate and is relatively refractory to antibiotic therapy. we report a case of fatal r. equi pneumonia in a normal host.19947852068
cytologic appearance of rhodococcus equi in bronchoalveolar lavage specimens. a case report.immunocompromised patients are at risk of developing infections caused by rhodococcus equi, a gram-positive bacillus that can cause pneumonia in patients with acquired immunodeficiency syndrome. to the author's knowledge this is the first report describing the cytologic features of the infection in a patient who had a confirmatory tissue biopsy and positive culture. infection with r equi may go unrecognized by pathologists unaware of its presentation and appearance in cytologic material.19957846998
rhodococcus equi infection in hiv-positive patients: report of 5 cases and literature overview.the importance of rhodococcus equi infection, an uncommon human pathogen that almost exclusively affects immunocompromised hosts, has greatly increased following the advent of acquired immune deficiency syndrome (aids) epidemics. until the present time, 38 cases of r. equi infection have been described in human immunodeficiency virus (hiv)-infected patients; we now report a further five personal cases. r. equi was acquired via respiratory exposure to animals in less than half of the patients, an ...19947841972
degradation of the thiocarbamate herbicide eptc (s-ethyl dipropylcarbamothioate) and biosafening by rhodococcus sp. strain ni86/21 involve an inducible cytochrome p-450 system and aldehyde dehydrogenase.determination of the n-terminal sequences of two eptc (s-ethyl dipropylcarbamothioate)-induced proteins from thiocarbamate-degrading rhodococcus sp. strain ni86/21 resolved by two-dimensional electrophoresis enabled the localization of the respective structural genes on two distinct dna fragments. one of these strongly induced proteins is a nad(+)-dependent dehydrogenase active on aliphatic aldehydes. the second protein was identified as a cytochrome p-450 enzyme. the cytochrome p-450 gene repre ...19957836301
[rhodococcus equi pneumonia in patients with aids]. 19947827773
primary subcutaneous abscess caused by rhodococcus equi. 19957825776
photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by fourier transform infrared spectroscopy.nitrile hydratase (nhase) from rhodococcus sp. n-771 is a photosensitive enzyme that catalyzes hydration of nitriles to the corresponding amides. light-induced fourier transform infrared difference spectra between the inactive and active forms of nhase were measured with both the natural (14n) and 15n-labeled nhases. the results showed, for the first time, that nhase intrinsically possesses nitric oxide (no) molecules bound to the non-heme iron center. the possible role of no in the photoactivat ...19957821438
opacification of middlebrook agar as an aid in distinguishing nocardia farcinica within the nocardia asteroides complex.among 58 aerobic actinomycetes isolated from different sources and geographical locations, none of 23 nocardia asteroides isolates, at 18 n. farcinica isolates, 1 of 5 n. otitidiscaviarum isolates, and 1 of 4 rhodococcus species isolates opacified middlebrook 7h10 medium. within the n. asteroides complex, this characteristic, together with growth at 45 degrees c and resistance to each of erythromycin, cefotaxime, and tobramycin, provides a simple means of distinguishing n. farcinica from n. aste ...19947814557
chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp. kinetic and immunochemical comparison with analogous enzymes from gram-negative strains.chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp was purified to homogeneity. in contrast to chlorocatechol 1,2-dioxygenase from gram-negative strains which have a very broad substrate tolerance, the rhodococcus enzyme was relatively more specific and had a distinct preference for 4-substituted catechols. protein and metal analysis indicate an unusual stoichiometry of one atom each of iron and manganese/64-kda homodimer. the n-terminal amino acid sequence (27 residues) of the rho ...19947813460
interaction of virulent and non-virulent rhodococcus equi human isolates with phagocytes, fibroblast- and epithelial-derived cells.rhodococcus equi is a facultative, intracellular, gram-positive coccobacillus, increasingly reported in pneumonia of aids-infected patients. we investigated killing resistance properties of human r. equi virulent and avirulent human strains. avirulent beta-lactam-susceptible strains had lower intracellular colony forming units after 45 min incubation in murine macrophages j774 and human monocyte-macrophage tph-1 than those of virulent strains. only virulent beta-lactam-resistant strains persiste ...19947812268
[rhodococcus equi pneumonia in a patient with aids. primary resistance to imipenem]. 19947811779
[rhodococcus equi pneumonia in patients with immunodeficiency].in three patients, men of 30, 21 and 24 years old respectively, rhodococcus equi pneumonia was diagnosed. antibiotic treatment, followed by secondary prophylaxis, was successful. in one patient, lobectomy was done. r. equi, a well-known bacterial pathogen in horses, appears also to be pathogenic in humans in case of immunodeficiency.19947808528
biochemical and serological characteristics of rhodococcus equi isolates from animals and humans.in the present study, 17 rhodococcus equi isolates from animals and 13 r. equi isolates from humans were correctly identified by the use of the api coryne-test system. the biochemical characteristics revealed no significant differences between isolates from animals and humans. heat extractions at acid ph or autoclave extractions of the bacteria and the use of monospecific antisera against type antigens 1-7 allowed the serotyping of the bacteria. the majority of the r. equi isolates reacted with ...19947801718
active-site analysis of ferric p450 enzymes: hydrogen-bonding effects on the circular dichroism spectra.active-site cd spectra were analyzed for p450's with known x-ray structures (p450terp, p450bm-3, p450cam). cd spectral patterns for fe(3+)-substrate-free p450's reflect structure/function properties of the distal pocket. p450terp and p450bm-3 have an h-bond between 6th ligand and i-helix [hasemann, c.a., et al. (1995) structure, 3, 41-62], and the soret cd band at approximately 410 nm is approximately 2-fold larger than that at approximately 350 nm. for p450cam, the two cd bands are more nearly ...19957794248
characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader rhodococcus sp. strain rha1.rhodococcus sp. strain rha1 is a gram-positive polychlorinated biphenyl (pcb) degrader which can degrade 10 ppm of pcb48 (equivalent to aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in a few days. we isolated the 7.6-kb ecori-bamhi fragment carrying the biphenyl catabolic genes of rha1 and determined their nucleotide sequence. on the basis of deduced amino acid sequence homology, we identified six bph genes, bpha1a2a3a4, bphb, and bphc, that are responsible for the initial thre ...19957793929
what is your diagnosis? osteomyelitis, septic arthritis. 19957782239
distribution and characterization of beta-lactamases of mycobacteria and related organisms.the detailed distribution and precise features of mycobacterial beta-lactamases urgently need to be elucidated.19957780097
rhodococcal abortion and pneumonia in an equine fetus. 19957779954
metabolism of halohydroquinones in rhodococcus chlorophenolicus pcp-1.the actinomycete rhodococcus chlorophenolicus pcp-1 metabolizes pentachlorophenol into ultimate inorganic end products via tetrachloro-p-hydroquinone. this intermediate was further dehalogenated in the cytoplasm requiring reductant in the cell free system. tetrafluoro-p-hydroquinone and tetrabromo-p-hydroquinone were also dehalogenated. chlorophenol analogs, thiol blocking agents and molecular oxygen inhibited the activity. the dehalogenating reactions led to 1,2,4-trihydroxybenzene, which was f ...19957772938
radiologic findings in two aids patients with rhodococcus equi pneumonia.rhodococcus equi (r. equi) has been reported as an occasional cause of pulmonary infection in severely immunocompromised hosts, including aids patients. our purpose is to describe the radiologic findings in two aids patients with r. equi pneumonia. chest radiographs showed right-upper-lobe consolidation and cavitation in both patients. chest ct confirmed upper mediastinal involvement and precarinal lymphadenopathy in both cases. multiple lung nodules related to the bronchi were also identified i ...19957769626
nitrile hydratase gene from rhodococcus sp. n-774 requirement for its downstream region for efficient expression.for improvement of the production of nitrile hydratase (nhase) from rhodococcus sp. n-774 by recombinant dna techniques, several plasmids, each of which had a deletion of the upstream or downstream region of the genes encoding the alpha and beta subunits of nhase, were constructed. enzyme assays of recombinant r. rhodochrous and escherichia coli cells showed that a downstream region of the nhase genes was indispensable for the production of active nhase in both cells, but for the production of t ...19947765511
production of a bioflocculant by mixed culture.colony groups that form large amounts of slime externally were obtained from activated sludge among phthalate-assimilating microbes. that slime, which r-3 mixed microbes produced externally, had a high level of flocculation activity. r-3 mixed strains, one of such colony groups, efficiently produced a bioflocculant (apr-3) in liquid cultures of production medium, especially that containing starch and glucose (1:1) as carbon sources. identification of this group of bioflocculant-producing microbe ...19947765478
purification and characterization of a novel carbonyl reductase isolated from rhodococcus erythropolis.during growth on n-tetradecane a novel nadh-dependent carbonyl reductase is induced in the gram-positive bacterium rhodococcus erythropolis (peters, p., zelinski, t. and kula, m.r. (1992) appl. microbiol. biotechnol. 38, 334-340). the enzyme has been purified to homogeneity using fractional ph precipitation, anion exchange chromatography and affinity chromatography. the isoelectric point of the oxidoreductase is 4.4. the apparent molecular mass of the native enzyme is 161 kda, that of the subuni ...19947764739
enantioselective hydrolysis of racemic naproxen nitrile and naproxen amide to s-naproxen by new bacterial isolates.bacteria were enriched from soil samples with succinate as a carbon source and racemic naproxen nitrile [2-(6-methoxy-2-naphthyl)propionitrile] as sole source of nitrogen. since naproxen nitrile was only poorly soluble in water media amended with different water-immiscible organic phases were used for the enrichments. with pristane (2,6,10,14-tetramethylpentadecane) as the organic phase two bacterial strains were isolated (strain c3ii and strain mp50) which were identified as rhodococci. cells o ...19947764731
production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile by the combination of nitrile hydratase and stereoselective amidase in rhodococcus equi tg328.a new soil isolate, tentatively identified as rhodococcus equi tg328, was found to be effective in the production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile. the conversion is catalysed by two enzymes. first, a nitrile hydratase converts the (r,s)-nitrile to (r,s)-2-phenylpropionamide. second, a stereoselective amidase converts the s-(+)-amide to s-(+)-2-phenylpropionic acid. conditions for optimal enzyme production and accumulation of s-(+)-2-phenylpropionic acid by restin ...19937764117
characterization of nitrile hydratase genes cloned by dna screening from rhodococcus erythropolis.southern hybridization analysis using the genes encoding the alpha- and beta-subunits of nitrile hydratase (nhase) from rhodococcus sp. n-774 as probe suggested that two r. erythropolis strains, jcm6823 and jcm2892, among 31 strains mainly from japan culture of microorganisms (jcm) have nhase genes. restriction analysis of dna fragments showing positive hybridization showed that each fragment carried a nucleotide sequence very similar to that of the nhase genes from rhodococcus sp. n-774. nucleo ...19937764017
development of an enzymatic process for manufacturing acrylamide and recent progress. 19937763351
[the fist detection of giardia spp. in horses in the czech republic].the first occurrence of giardia spp. in horses in the czech republic is reported. during preventive examination of 360 five-month up to 14-year horses from various parts of the region of central bohemia carried out from january 1993 to june 1994 in the parasitological laboratory of the state veterinary institute in prague, the giardia cysts were detected in the excrements of 18 (5%) horses, mostly 2-4 years of age, and in two foals 3 and 6 weeks old. during the period between march 1993 and june ...19957762123
chronic osteitis due to rhodococcus equi in an immunocompetent patient. 19957756506
characterization of muconate and chloromuconate cycloisomerase from rhodococcus erythropolis 1cp: indications for functionally convergent evolution among bacterial cycloisomerases.muconate cycloisomerase (ec 5.5.1.1) and chloromuconate cycloisomerase (ec 5.5.1.7) were purified from extracts of rhodococcus erythropolis 1cp cells grown with benzoate or 4-chlorophenol, respectively. both enzymes discriminated between the two possible directions of 2-chloro-cis, cis-muconate cycloisomerization and converted this substrate to 5-chloromuconolactone as the only product. in contrast to chloromuconate cycloisomerases of gram-negative bacteria, the corresponding r. erythropolis enz ...19957751292
cauda equina syndrome, diskospondylitis, and a paravertebral abscess caused by rhodococcus equi in a foal.a 4-month-old male quarter horse was referred for evaluation of urinary incontinence. physical examination revealed clinical signs consistent with cauda equina syndrome. radiography revealed diskospondylitis of s2 through s4. infected bone was surgically curretted, and drainage was established for an associated paravertebral abscess. rhodococcus equi was isolated from specimens of bone and from fluid samples obtained from the paravertebral abscess. bethanechol was administered to stimulate urina ...19957751225
characterization of the bacillus stearothermophilus br219 phenol hydroxylase gene.the catabolic genes phea and pheb, coding for the conversion of phenol to catechol and catechol to 2-hydroxymuconic semialdehyde, respectively, have been cloned from bacillus stearothermophilus br219 into escherichia coli. following its localization on the 11-kb b. stearothermophilus dna insert by deletion and expression analysis, the phenol hydroxylase gene phea was subcloned as a 2-kb hindiii fragment, whose transformant expressed the enzyme after phenol induction and even more strongly after ...19957747948
disseminated abscesses caused by rhodococcus equi in a patient with chronic lymphocytic leukemia. 19957742468
rhodococcus equi--an increasingly recognized opportunistic pathogen. report of 12 cases and review of 65 cases in the literature.rhodococcus equi, a gram-positive, weakly acid-fast coccobacillus, initially isolated from horses, is becoming increasingly recognized as an important pathogen for immunosuppressed human hosts since the first human case was reported in 1967. a review of the english medical literature yielded 53 cases. during the last 11 years, the microbiology laboratories of the authors isolated the organism from 12 patients. of the total 65 cases, 60 occurred in immunosuppressed patients with hiv infection, ma ...19957741114
the evolutionary relationship of biphenyl dioxygenase from gram-positive rhodococcus globerulus p6 to multicomponent dioxygenases from gram-negative bacteria.the gram+ bacterium rhodococcus globerulus p6 (rgp6) catabolizes a range of polychlorinated biphenyl (pcb) congeners, thus being of interest in bioelimination processes for pcb. the first step in the pathway, a dioxygenase attack of one of the biphenyl (bp) rings, is catalyzed by biphenyl dioxygenase (bdo). in this study, the nucleotide (nt) sequences of the four clustered cistrons, bpha1a2a3a4, encoding the subunits of bdo and forming part of the bph operon of rgp6 for bp degradation, were dete ...19957737502
identification of the region of a 14-kilodalton protein of rhodococcus ruber that is responsible for the binding of this phasin to polyhydroxyalkanoic acid granules.the function of the polyhydroxyalkanoic acid (pha) granule-associated ga14 protein of rhodococcus ruber was investigated in escherichia coli xl1-blue, which coexpressed this protein with the polyhydroxybutyric acid (phb) biosynthesis operon of alcaligenes eutrophus. the ga14 protein had no influence on the biosynthesis rate of phb in e. coli xl1-blue(pskco7), but this recombinant e. coli strain formed smaller phb granules than were formed by an e. coli strain that expressed only the phb operon. ...19957730285
analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in alcaligenes eutrophus.a 5.0-kbp genomic ecori restriction fragment which complemented a third subclass of polyhydroxyalkanoic acid (pha)-leaky mutants of a. eutrophus that accumulated pha at a lower rate than the wild type was cloned from alcaligenes eutrophus h16. a 687-bp phapae gene on this fragment encoded a 24-kda protein (m(r) = 23,963), which was referred to as the ga24 protein. the ga24 protein was solubilized from the granules and purified to electrophoretic homogeneity, and antibodies against the ga24 prote ...19957730274
rhodococcus meningitis in an immunocompetent host.rhodococcus species are increasingly being recognized as pathogens, especially in patients infected with the human immunodeficiency virus. most cases of rhodococcus infection in these patients are due to rhodococcus equi and involve the lungs. cns infections due to rhodococcus species are rare, and meningitis due to non-equi rhodococcus has never been reported in a healthy host. we report a case of meningitis due to non-equi rhodococcus in a previously healthy 24-year-old woman. we also review a ...19957727645
alpha-mannosidase: a rapid test for identification of arcanobacterium haemolyticum.a 4-h alpha-mannosidase test for identification of arcanobacterium haemolyticum strains (n = 139) and differentiation of a. haemolyticum from actinomyces pyogenes strains (n = 30) and other gram-positive rods was evaluated. practically all a. haemolyticum strains (138 of 139) and the listeria monocytogenes type strain were alpha-mannosidase positive, while all a. pyogenes strains and corynebacterium (n = .25) strains as well as the rhodococcus equi and erysipelothrix rhusiopathiae type strains w ...19947710479
molecular characterization of a lipid-modified virulence-associated protein of rhodococcus equi and its potential in protective immunity.virulent strains of rhodococcus equi produce plasmid-mediated 15- and 17-kda proteins, which are thermoregulated and apparently surface-expressed. we demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) that r. equi produce three antigenically-related virulence-associated proteins, a diffuse 18-22-kda, a 17.5-kda and a 15-kda protein. phase partitioning of whole cells of r. equi strain 103 with triton x-114 (tx-114) and labelling with [3h]-labelled palmitic acid s ...19957704843
establishment of oil-degrading bacteria associated with cyanobacteria in oil-polluted soil.a unique natural microbial cocktail with promising potential for remediating oil-polluted desert in the gulf region is reported. oil-degrading micro-organisms immobilized within dense cyanobacterial mats on oily coasts of the arabian gulf were successfully established in oil-contaminated sand. those micro-organisms biodegraded 50% of the oil within 10-20 weeks. nocardioforms belonging to the genus rhodococcus predominated in the first few weeks, but after 22 weeks pseudomonas spp. increased, sha ...19957698954
sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens.the nucleotide sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens, located on plasmid preat701, has been determined. the gene encodes a 19-kda protein of 189 amino acids, with an ala-rich leader signal sequence (ss). at least five ss peptidase cleavage sites were found in this region. the molecular diversity of 15-17-kda antigens might be attributed to the multiple ss peptidase cleavage sites.19957698659
rhodococcus species fatal infection in an immunocompetent host.a 24-year-old woman had fatal pneumonia-associated adult respiratory distress syndrome caused by rhodococcus species. histological examination of lung biopsy tissue showed intracellular coccobacillary microorganisms. antimicrobial susceptibility tests on the patient's blood isolate showed that it was resistant to clindamycin and norfloxacin but susceptible to several other antimicrobial agents. also, the isolate's biochemical reactions and pattern of rna gene-containing restriction fragments wer ...19937683869
cloning, sequence analysis, and expression of the flavobacterium pentachlorophenol-4-monooxygenase gene in escherichia coli.the pcpb gene of flavobacterium sp. strain atcc 39723 was cloned by using a degenerate primer designed from the n-terminal sequence of the purified enzyme. the nucleotide sequence of pcpb was determined and found to encode an open reading frame of 1,614 nucleotides, yielding a predicted translation product of 538 amino acids, in agreement with the estimated size of the purified protein analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the transcriptional start of pcpb was fo ...19937678243
infections due to rhodococcus equi in three hiv-infected patients: microbiological findings and antibiotic susceptibility.infections of rhodococcus equi, a well-known pathogen in animals which causes cavitated pneumonia similar to that caused by mycobacteria, were studied in three hiv-infected patients. this microorganism was isolated in the bronchoalveolar washings of two patients and in the sputum of the third. in two patients, rh. equi represented the first clinical opportunistic manifestation of hiv disease. one patient died of concomitant pneumocystis infection. the eradication of the microorganism occurred in ...19947672872
bacterial steroid monooxygenase catalyzing the baeyer-villiger oxidation of c21-ketosteroids from rhodococcus rhodochrous: the isolation and characterization.steroid monooxygenase from rhodococcus rhodochrous, isolated in homogeneity with a high yield, catalyzes baeyer-villiger oxidation of progesterone to produce testosterone acetate with the stoichiometric consumptions of nadph and molecular oxygen. it is a flavoenzyme with the molecular size of 60 kda in the monomeric form and the isoelectric point of 4.9. the absorption spectrum has the maxima at 278, 376, and 439 nm and the shoulders at 360 and 465 nm, indicating a strong hypsochromic shift (blu ...19957669800
clinical pharmacologic aspects of cefixime in dogs.the minimal inhibitory concentration (mic) of cefixime, a new third-generation orally administered caphalosporin, was determined for reference and clinical isolates from dogs. the mic of the drug for all but 1 of the 18 enterobacteriaceae isolates tested, 1 pasteurella canis, 1 rhodococcus equi, 1 streptococcus canis, and 1 streptococcus group g isolate, was less than 1.0 microgram/ml. the mic for 9 staphylococcus intermedius isolates ranged from 1.56 to 6.25 micrograms/ml and, for 8 sta aureus ...19957661460
machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols.six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ...19957656609
development of a new selective plating medium for rhodococcus equi.a new selective plating medium for rhodococcus equi containing ceftazidime (20 mg/l) and novobiocin (25 mg/l) on a mueller-hinton agar basis is described. it proved to be less inhibitory for r. equi than selective plating media devised earlier and grew only very few other nocardioform bacteria.19957651242
identification of virulent rhodococcus equi by amplification of gene coding for 15- to 17-kilodalton antigens.during a survey of the prevalence of virulent rhodococcus equi at horse-breeding farms by plasmid and protein profiles, cryptic plasmids of various sizes were found in 66 (3.8%) of 1,725 isolates from feces of horses and 129 (5.9%) of 2,200 isolates from soil. twenty-two isolates, which contained cryptic plasmids of different sizes, were found by plasmid profiles, and their protein profiles and mouse pathogenicities were examined. of the 22 isolates, 7 were virulent r. equi, contained both virul ...19957650199
cloning of the genes for degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine from rhodococcus sp. strain te1.the degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in rhodococcus sp. strain te1. plasmid dna libraries of rhodococcus sp. strain te1 were constructed in a rhodococcus-escherichia coli shuttle vector, pbs305, and transferred into rhodococcus sp. strain te3, a derivative of rhodococcus sp. strain te1 lacking herbicide degradation activity, to select transformants capa ...19957646050
a single cytochrome p-450 system is involved in degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine by rhodococcus sp. strain ni86/21.during atrazine degradation by rhodococcus sp. strain n186/21, n-dealkylated metabolites and an hydroxyisopropyl derivative are produced. the cytochrome p-450 system that is involved in degradation of thiocarbamate herbicides by strain n186/21 (i. nagy, g. schoofs, f. compernolle, p. proost, j. vanderleyden, and r. de mot, j. bacteriol. 177:676-687, 1995) is also required for atrazine degradation. atrazine-degrading activity was conferred on the atrazine-negative strains, mutant faj2027 of rhodo ...19957646049
identification of two new genes in the pseudomonas aeruginosa amidase operon, encoding an atpase (amib) and a putative integral membrane protein (amis).the nucleotide sequence of the amidase operon of pseudomonas aeruginosa has been completed and two new genes identified amib and amis. the complete gene order for the operon is thus amiebcrs. the amib gene encodes a 42-kda protein containing an atp binding motif that shares extensive homology with the clp family of proteins and also to an open reading frame adjacent to the amidase gene from rhodococcus erythropolis. deletion of the amib gene has no apparent effect on inducible amidase expression ...19957642533
identification and activity of two insertion sequence elements in rhodococcus sp. strain igts8.two putative insertion sequence (is) elements, is1166 and is1295, were identified on a plasmid present in rhodococcus sp. igts8. four copies of is1166 were present in strain igts8: one copy on each of two separate plasmids and two copies on a third plasmid or in the chromosome. of eight rhodococci tested, only r. zopfii contained a copy of an is1166-like element. two mutants of strain igts8 were isolated in which an additional copy of is1166 was present, suggesting that at least one copy of this ...19957642133
aliphatic nitrilase from a soil-isolated comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure.an aliphatic nitrilase, active on adiponitrile and cyanovaleric acid, was identified and purified from comamonas testosteroni sp. (ct). oligodeoxyribonucleotide probes were designed from limited amino acid (aa) sequence information and used to clone the corresponding gene, named nita. high homologies were found at the aa level between ct nitrilase and the sequences of known nitrilases. multi-alignment of sequenced nitrilases suggests that cys163 of ct plays an essential role in the active site. ...19957642130
characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil.in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ...19957641143
another cause of endobronchial lesions found in hiv patients. 19957634912
[susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine and combinations of these compounds].using a broth microdilution technique, the in vitro susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine, and combinations of these compounds was determined. the bacterial strains (n = 88) isolated recently from horses with respiratory symptoms belonged to the following species: streptococcus equi subsp. zooepidemicus (n = 34), streptococcus equi subsp. equi (n = 22), staphylococcus aureus (n = 9), klebsiella pneumoniae (n = 7), rh ...19957624856
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