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ligand binding and substrate discrimination by udp-galactopyranose mutase.galactofuranose (galf) residues are present in cell wall glycoconjugates of numerous pathogenic microbes. uridine 5'-diphosphate (udp) galf, the biosynthetic precursor of galf-containing glycoconjugates, is produced from udp-galactopyranose (udp-galp) by the flavoenzyme udp-galactopyranose mutase (ugm). the gene encoding ugm (glf) is essential for the viability of pathogens, including mycobacterium tuberculosis, and this finding underscores the need to understand how ugm functions. considerable ...200919500588
acute suppurative klebsiella thyroiditis: a case report.acute suppurative thyroiditis and thyroid abscess are extremely rare disorders. the most common pathogens causing acute suppurative thyroiditis are gram-positive bacteria, including staphylococcal and streptococcal species. thyroid abscess is mostly located in the left thyroid lobe. we report the case of a 75-year-old female patient with acute suppurative thyroiditis and right lobe thyroid abscess caused by klebsiella spp. the patient had a firm, livid, hardly mobile cervical swelling. axial com ...200919499695
activity of the anti-mrsa carbapenem razupenem (ptz601) against enterobacteriaceae with defined resistance mechanisms.razupenem (previously known as ptz601, pz-601, smp-601 or sm-216601) is a novel carbapenem, active against enterobacteriaceae as well as gram-positive bacteria including methicillin-resistant staphylococci and enterococci.200919497942
pneumonia in a cardiothoracic intensive care unit: incidence and risk factors.the purpose of this study was to determine the incidence, risk factors, and pathogens causing pneumonia in a cardiothoracic intensive care unit (cticu).200919497769
characterization of ctx-m esbls in enterobacter cloacae, escherichia coli and klebsiella pneumoniae clinical isolates from cairo, egypt.a high rate of resistance to 3rd generation cephalosporins among enterobacteriaceae isolates from egypt has been previously reported. this study aims to characterize the resistance mechanism (s) to extended spectrum cephalosporins among resistant clinical isolates at a medical institute in cairo, egypt.200919497111
carbapenem resistance among klebsiella pneumoniae isolates: risk factors, molecular characteristics, and susceptibility patterns.carbapenem resistance among isolates of klebsiella pneumoniae has been unusual.200919496647
new thioureides of 2-(4-methylphenoxymethyl) benzoic acid with antimicrobial activity.the purpose of this study was to evaluate in vitro, by means of qualitative and quantative methods, the antimicrobial activity of some new synthesized chemical compounds previously solubilized in dmf (dimethyl formamide). the qualitative screening of the susceptibility spectra of different microbial strains versus these compounds was performed by three adaptated diffusion methods: paper filter disk impregnation with the tested substances solutions, the disposal of tested solutions in agar wells ...200819496477
molecular identification of (bla)shv, (bla)len and (bla)okp beta-lactamase genes in klebsiella pneumoniae by bi-directional sequencing of universal sp6- and t7-sequence-tagged (bla)shv-pcr amplicons.plasmid encoded (bla)shv enzymes represent an important sub-group of class a beta-lactamases causing an esbl-phenotype which is increasingly found in enterobacteriaceae including klebsiella pneumoniae. the chromosomally encoded beta-lactamase (bla)len and (bla)okp enzymes, which so far only have been found in k. pneumoniae, do not hydrolyse extended-spectrum cephalosporins. in the present study, multiple displacement amplified dna derived from 20 k. pneumoniae clinical isolates with a (bla)shv-l ...200919496249
cephalosporin mic distribution of extended-spectrum-{beta}-lactamase- and pampc-producing escherichia coli and klebsiella species.the acquisition of beta-lactamases in members of the enterobacteriaceae family poses a challenge to antimicrobial susceptibility testing in the clinical laboratory. we correlated the distribution of the mics for klebsiella spp. and escherichia coli with the presence of extended-spectrum beta-lactamase (esbl) and plasmid-mediated ampc beta-lactamase (pampc) genes. a total of 264 isolates were subjected to cefazolin, ceftriaxone, cefotaxime, ceftazidime, cefepime, and aztreonam agar dilution mic d ...200919494061
the microbiota of lafun, an african traditional cassava food product.lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ...200919493582
transconjugation and genotyping of the plasmid-mediated ampc beta-lactamase and extended-spectrum beta-lactamase genes in klebsiella pneumoniae.ampc beta-lactamases and extended-spectrum beta-lactamases (esbls) are becoming predominant causes of resistance to third and forth-generation cephalosporins in klebsiella pneumoniae (k. pneumoniae). it is very difficult to treat infectious diseases caused by multidrug-resistant k. pneumoniae. the purpose of the present study was to investigate transconjugation and characteristics of beta-lactamase genes in k. pneumoniae producing ampc beta-lactamases and esbls.200919493446
application of a novel biopolymer for removal of salmonella from poultry wastewater.this study evaluated the potential of an extracellular, novel biopolymeric flocculant produced by a strain of klebsiella terrigena for removal of salmonella, a potent pathogen prevalent in poultry wastewater. the purified biopolymer was applied to poultry wastewater containing 3 log cfu cells of salmonella. an optimized dosage of 2 mg l(-1) of the purified bioflocculant was sufficient to remove 80.3% salmonella spp. within 30 min, at ambient temperature. also this bioflocculant showed high flocc ...200919492545
optic neuritis after klebsiella pneumonitis and liver abscess.a 56-year-old woman developed a left optic neuropathy in the context of a klebsiella pneumonitis that had also produced a liver abscess. ophthalmic examination was normal apart from no light perception vision in the left eye and a left afferent pupil defect. orbit and brain mri revealed enhancement of the left optic nerve and several round enhancing areas in the gray-white junction of the frontal and temporal lobes consistent with microabscesses. although the patient recovered systemically with ...200919491637
klebsiella pneumoniae carbapenemase-producing enterobacteriaceae, northeast florida.since 2001 there have been several reported outbreaks due to carbapenem-resistant klebsiella pneumoniae (kp), particularly in the northeastern states.200919487990
subphrenic abscess secondary to actinomycosis meyeri and klebsiella ozaenae following laparoscopic cholecystectomy.a case is reported of a subphrenic abscess 12 months post-laparoscopic cholecystectomy in a 72-year-old male with identification of actinomyces meyeri and the oropharyngeal commensal klebsiella ozaenae. the first organism is exceptionally rare following laparoscopic cholecystectomy and is presumed to be a result of inadvertent gallstone spillage. the second organism has not previously been reported in a subphrenic abscess. the etiopathogenesis and management of this condition are presented.200919487988
[mutant prevention concentrations of antibacterial agents to ocular pathogenic bacteria].to establish a method to measure mutant prevention concentration (mpc) in vitro, and to measure mpc of antibacterial agents for ocular bacteria caused keratitis.200919484933
tetracycline and chloramphenicol efficiency against selected biofilm forming bacteria.despite the constantly increasing need for new antimicrobial agents, antibiotic drug discovery and development seem to have greatly decelerated in recent years. presented with the significant problem of advancing antimicrobial resistance, the global scientific community has attempted to find alternative solutions; one of the most promising ones is the evaluation and use of old antibiotic compounds. a number of old antibiotic compounds, such as aminoglycosides, chloramphenicol, and tetracycline, ...200919484302
characterization of a t7-like lytic bacteriophage of klebsiella pneumoniae b5055: a potential therapeutic agent.characterization of bacteriophages to be used prophylactically or therapeutically is mandatory, as use of uncharacterized bacteriophages is considered as one of the major reasons of failure of phage therapy in preantibiotic era. in the present study, one lytic bacteriophage, kpo1k2, specific for klebsiella pneumoniae b5055, with broad host range was selected for characterization. as shown by tem, morphologically kpo1k2 possessed icosahedral head with pentagonal nature with apex to apex head diam ...200919484297
screening of platelet concentrates for bacterial contamination: spectrum of bacteria detected, proportionof transfused units, and clinical follow-up.screening of platelet concentrates (pcs) for bacterial contamination with cultivation methods is carried out as a routine procedure in some countries. the aim is to prevent the transfusion of contaminated pcs. the german evaluation of regular monitoring study group conducted a prospective multicenter study on 52,243 pcs to investigate the prevalence of bacteria (bact/alert, biomerieux). this study describes the detected bacterial spectrum, the proportion of pcs with a positive test result that h ...200919484239
septicemia complicating rotavirus gastroenteritis in 5-year-old child. 200919483530
novel furanylarylene arylsulfonylindolesulfonamides: synthesis and their antibacterial evaluation.an array of furanylarylene arylsulfonylindolesulfonamides was synthesized through multi-step synthetic protocols involving bromination, stannylation, stille cross coupling, reduction, arylsulfonylation, chlorosulfonylation, and condensation reactions. as a preliminary evaluation, these analogs were tested for antibacterial activity against a series of bacterial strains such as bacillus subtilis, enterococcus faecalis, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and klebsiell ...200919483339
prevalence of plasmid-mediated quinolone resistance determinants in esbl enterobacteriaceae clinical isolates over a 1-year period in a french hospital.the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants (qnra, qnrb, qnrs, aac(6')-ib-cr, and qepa) was investigated in a collection of 47 extended-spectrum beta-lactamase (esbl) producing enterobacterial isolates with reduced susceptibility to fluoroquinolones, recovered at nantes university hospital, in 2006. qnr, aac(6')-ib-cr, and qepa genes were screened by pcr, and positive results were subsequently confirmed by sequencing. the epidemiological relationship between posit ...200919481883
bacterial translocation after mild therapeutic hypothermia. 200919481854
synthesis and in vitro antibacterial activity of 7-(4-alkoxyimino-3-amino-3-methylpiperidin-1-yl)fluoroquinolone derivatives.a series of novel 7-(4-alkoxyimino-3-amino-3-methylpiperidin-1-yl)fluoroquinolone derivatives were designed, synthesized and characterized by (1)h nmr, ms and hrms. these fluoroquinolones were evaluated for in vitro antibacterial activity against representative gram-positive and gram-negative strains. all of the title compounds have considerable activity against the twelve strains, and exhibit exceptional potency in inhibiting the growth of staphylococcus aureus, staphylococcus epidermidis and k ...200919481308
kpc-2-producing klebsiella pneumoniae infections in greek hospitals are mainly due to a hyperepidemic clone.to verify the presence of klebsiella pneumoniae carbapenemase-producing (kpc-producing) klebsiella pneumoniae in greece, we asked 40 greek hospitals participating in the greek system for the surveillance of antimicrobial resistance (gssar) to apply a combination of the modified hodge test plus edta synergy test on all k. pneumoniae clinical isolates obtained from february 2008 which displayed reduced susceptibility to carbapenems (mic of imipenem > or = 1 mg/l). the presence of the blakpc gene w ...200919480809
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
incidence of multiple antibiotic resistant gram-negative bacteria isolated from surface and underground water sources in south western region of nigeria.in most rural and urban settlements, particularly in nigeria, wells, spring, streams or rivers and lakes serves as major sources of water supply for drinking and other domestic purposes. unfortunately, many of the available water sources are not potable without some form of treatment which is seldom available in most settings. the use of untreated surface water sources for drinking and for domestic purposes remains a major threat to public health as these could serve as reservoirs the for transf ...200919474486
[comparative assessment of the vitek 2 and phoenix systems for detection of extended-spectrum beta-lactamases].detection of beta-lactam resistance in escherichia coli and klebsiella pneumoniae strains is clinically relevant. moreover, it is important to differentiate between extended-spectrum beta-lactamase (esbl) production and other mechanisms of resistance to avoid inadequate treatment of infection caused by these strains. the aim of this study was to compare the performance of the vitek 2 and bd phoenix automated systems for confirmatory testing of esbl production.200919473732
chemical compositions and antibacterial activities of the essential oils from aerial parts and corollas of origanum acutidens (hand.-mazz.) ietswaart, an endemic species to turkey.essential oils extracted by hydrodistillation from the aerial parts and corollas of origanum acutidens (hand.-mazz.) ietswaart, an endemic turkish flora species, were analyzed by gc-ms. the amounts of essential oil obtained from the aerial parts and the corollas were 0.73% and 0.93%, respectively. twenty-five components in both the aerial parts oil and the corolla oil, representing 95.11% and 93.88%, respectively, were identified. the aerial parts and corolla oils were characterized by the predo ...200919471191
[implementation of antimicrobial resistance surveillance and antibiotic consumption in an intensive care burn department].the increasing consumption of antibiotics in hospitals and the economic implications of this increase lead to survey this consumption in the various hospital units.200819469305
[epidemiology of bacterial intrahospital infections in newborns].nosocomial infections are important problem for the modern medicine. especially sensitive category, susceptible to nosocomial infections, are the infants. the aim of the research was to establish an active monitoring over the breaking out of the nosocomial infections in the neonatology department of the gynecology and obstetrics clinic of the university clinical center tuzla (ukc) and to determine the kind of bacteria that causes the nosocomial infections as well as the type of infection accordi ...200819469274
[characteristics of broncho-obstruction in childhood].the prevalence of broncho-obstruction in children is in permanent increase at any age and in global. according to many studies, about 50% of children have at least one episode of broncho-obstruction in the first six years of life. risk factors for broncho-obstruction in children include not only intrinsic, but a lot of extrinsic factors which could accelerate beginning of early symptoms. globally, there is increase in first hospitalization with broncho-obstruction and many authors described decr ...200819469269
[microbiol landscape and production of beta-lactamases with extended spectrum activity in patients with urinary tract infection].urinary tract infections (utis) are widespread in russia. one of the major problems facing practical medicine in the country is laboratory diagnosis of pyoinflammatory diseases of the urogenital tract caused by opportunistic microorganisms. a major difficulty in this context is posed by polyresistance of certain gram-negative bacteria (e.coli, klebsiella spp., etc.) associated with their production of a great variety of beta-lactamases (bl). this study was designed to determine species compositi ...200919469259
antimicrobial activity of selected plant species from "the argentine puna" against sensitive and multi-resistant bacteria.the plant species reported here are traditionally used in the "puna" or "altiplano" of argentina for ailments related to bacterial infections. the aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant gram-positive and gram-negative bacteria.200919467313
activity of antimicrobial peptides in the presence of polysaccharides produced by pulmonary pathogens.antimicrobial peptides (amps) are secreted in the airway and contribute to initial defence against inhaled pathogens. infections of the respiratory tract are a major cause of morbidity and mortality in preterm newborns and in patients with cystic fibrosis (cf). in this latter group, the state of chronic lung infection is due to the ability of bacteria to grow as mucoid biofilm, a condition characterised by overproduction and release of polysaccharides (pss). in this study, we investigate the eff ...200919466693
outcomes of malignant external otitis: survival vs mortality.in patients with malignant external otitis (meo), mortality was not related to age, sex, degree of glucose tolerance, duration of diabetes mellitus (dm), microorganism, comorbid disease, or involvement of a single cranial nerve. severe complications such as skull base osteomyelitis, intracranial extension, and involvement of multiple cranial nerves were correlated with mortality.201019466617
gas-forming pyogenic liver abscess. 200919465375
antityrosinase and antimicrobial activities of trans-cinnamaldehyde thiosemicarbazone.tyrosinase (ec 1.14.18.1) is a key enzyme in pigment biosynthesis of organisms. trans-cinnamaldehyde thiosemicarbazone, a derivative of benzaldehyde thiosemicarbazone, was synthesized as an inhibitor of tyrosinase. the inhibitory effects of this compound on the activity of mushroom tyrosinase were investigated. the results showed that trans-cinnamaldehyde thiosemicarbazone could potently inhibit both monophenolase activity and diphenolase activity of tyrosinase. for monophenolase activity, trans ...200919462982
[choice of probiotic for rational therapy of infection caused by klebsiella in children].to choose lactobacilli-contained probiotic for complex treatment of acute enteric infection caused by klebsiella in infants.200919462517
[analysis of distribution, drug resistance and risk factors of pathogens isolated from septicemic patients].to investigate distribution, drug resistance and risk factors of pathogens isolated from septicemic patients in a hospital in the past 6 years.200919460728
effect of soft segment crystallization and hard segment physical crosslink on shape memory function in antibacterial segmented polyurethane ionomers.shape memory polyurethane (smpu) ionomers containing constant 75 wt.% soft segment content were synthesized using poly(epsilon-caprolactone)diol, 4,4'-diphenylmethane diisocyanate, 1,4-butanediol and/or n,n-bis(2-hydroxyethyl)-isonicotinamide. to introduce substrate bonding antibacterial activity, pyridinium was prepared through a neutralization reaction using 1-iodooctane as neutralization agent. for the smpu ionomer film obtained, tensile testing at 70 degrees c and dynamic mechanical analysis ...200919460466
mortality rate in neonates infected with extended-spectrum beta lactamase-producing klebsiella species and selective empirical use of meropenem.infection with resistant gram-negative bacteria is a growing threat to hospitalised patients.200919460263
activity of the antimicrobial peptide and thanatin analog s-thanatin on clinical isolates of klebsiella pneumoniae resistant to conventional antibiotics with different structures.the treatment of infections caused by bacteria resistant to the vast majority of antibiotics is a challenge worldwide. to evaluate the effect of s-thanatin (an analog of thanatin, a cationic antimicrobial peptide isolated from the hemipteran insect podisus maculiventris) against microbial resistant to antibiotics, we studied its bactericidal kinetics, synergistic effect, resistance, and activity on clinical isolates of klebsiella pneumoniae resistant to conventional antibiotics with different st ...200919459007
correlation of the expression of acrb and the regulatory genes mara, soxs and rama with antimicrobial resistance in clinical isolates of klebsiella pneumoniae endemic to new york city.nosocomial isolates of klebsiella pneumoniae resistant to all commonly used antimicrobial agents have emerged in many regions of the world. it is unknown if efflux systems contribute to the multidrug resistance phenotype.200919457933
detection of ampc beta-lactamases in escherichia coli, klebsiella spp., salmonella spp. and proteus mirabilis in a regional clinical microbiology laboratory.there are currently no standardized diagnostic tests available for the reliable detection of ampc beta-lactamases in klebsiella spp., escherichia coli, proteus mirabilis and salmonella spp. a study was designed to evaluate a confirmation disk test using cefotetan (ctt) and cefoxitin (fox) with phenylboronic acid (pba). it also investigated the most suitable screening concentrations of fox, ceftriaxone (cro) and ceftazidime (caz) for the detection of ampc beta-lactamases. a total of 126 control ( ...201019456838
cationic compounds with activity against multidrug-resistant bacteria: interest of a new compound compared with two older antiseptics, hexamidine and chlorhexidine.use of antiseptics and disinfectants is essential in infection control practices in hospital and other healthcare settings. in this study, the in vitro activity of a new promising compound, para-guanidinoethylcalix[4]arene (cx1), has been evaluated in comparison with hexamidine (hx) and chlorhexidine (chx), two older cationic antiseptics. the mics for 69 clinical isolates comprising methicillin-resistant staphylococcus aureus, methicillin-sensitive s. aureus, coagulase-negative staphylococci (co ...201019456831
extended-spectrum beta-lactamase klebsiella pneumoniae meningitis treated with tigecycline. 200919448375
genome sequencing and comparative analysis of klebsiella pneumoniae ntuh-k2044, a strain causing liver abscess and meningitis.nosocomial infections caused by antibiotic-resistant klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired k. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. in particular, an invasive form of k. pneumoniae that causes liver abscesses was first observed in asia and then was found worldwide. we are interested in how differences in gene content of the same species result in different diseases. thus, w ...200919447910
synthesis and antibacterial evaluation of isoxazolinyl oxazolidinones: search for potent antibacterial.a series of (5s) n-(3-{3-fluoro-4-[4-(3-aryl-4,5-dihydro-isoxazole-5-carbonyl)-piperazin-1-yl]-phenyl}-2-oxo-oxazolidin-5-ylmethyl)-acetamide(6a-o) were synthesized and their in vitro antibacterial activity against various resistant gram-positive and gram-negative bacteria were evaluated. most of the synthesized compounds showed 2 to 10 fold lower mic values compared to linezolid against staphylococcus aureusatcc 25923, atcc 70069, atcc 29213,bacillus cereusmtcc 430,enterococcus faecalismtcc439, ...200919447611
bacteriocin production by pediococcus pentosaceus isolated from marula (scerocarya birrea).strain st44am, isolated from marula, was identified as pediococcus pentosaceus based on biochemical tests, sugar fermentation reactions (api 50chl), pcr with species-specific primers and 16s rdna sequencing. strain st44am produces a 6.5 kda class iia bacteriocin, active against lactic acid bacteria, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, listeria innocua, listeria ivanovii subsp. ivanovii and listeria monocytogenes. the peptide is inactivated by proteolytic enzymes, but ...200919446352
[stellanin-peg 3% ointment: comparative antimicrobial activity against surgical infection pathogens].comparative antimicrobial activity of four ointments, i. e. stellanin-peg, levomecole, dioxydin 5% ointment and betadin was studied with the use of collection microbial strains and the most frequent pathogens of pyoinflammary processes in surgical patients. clinical strains of gram positive and gram negative organisms were used as the test cultures. stellanin-peg 3% ointment showed high antimicrobial activity against both the gram positive and the gram negative organisms, including methicillin r ...200819441651
preparation of biodegradable polymer/silver nanoparticles composite and its antibacterial efficacy.biodegradable poly(ethylene oxide) nanofibers containing silver nanoparticles were successfully prepared by the electrospinning without the further reduction process which could cause the damages of nanofibers. transmission electron microscopy showed that the silver nanoparticles were dispersed in poly(ethylene oxide) nanofibers without aggregation. this facile process would be the effective method to prepare the silver nanoparticles/polymer nanocomposites. moreover, the silver nanoparticles emb ...200919441464
[severe acute pancreatitis with pneumoperitoneum and retropneumoperitoneum]. 200919439282
[association of urinary tract infections and hiv infection in an internal medicine ward of hospital of point g-bamako-mali].we gathered 14 clinical observations of urinary track infections in aids patients in internal medicine wards from august 1, 1998 to july 31, 1999. urinary track infection and hiv were both present in 1.75% of 797 hospitalized patients. the specific prevalence was 14.43% among aids patients. the sex ratio (female/male) was 1.33. the mean age of patients was 37.2 years with ranges between 25 years and 59 years. urinary symptoms were discret. urinary tract infection was primarily evidenced by urine ...200719437827
[neonatal urinary tract infections in abidjan: the problem of bacterial resistance].background: neonatal infection is frequent with rate of lethality between 20 to 70% in the developing countries. among these neonatal infections, urinary infection is serious .the diagnosis of urinary infection is based on cytobacteriological exam. but it is rarely done for economic reasons. principal objective of this survey was to identify the germs responsible for the urinary infections at the newborn in order to propose an adequate therapeutic algorithm. study: from july to december 2004, 72 ...200819437812
community-acquired urinary tract pathogens and their resistance patterns in hospitalized children in southeastern ontario between 2002 and 2006.the purpose of the present study was to assess the epidemiology and resistance patterns of bacteria causing urinary tract infections in children who were admitted to kingston general hospital (kingston, ontario) - the regional tertiary care hospital of southeastern ontario.200819436535
high frequency of multidrug-resistant gram-negative rods in 2 neonatal intensive care units in the philippines.although hospital-acquired infections appear to be a growing threat to the survival of newborns in the developing world, the epidemiology of this problem remains poorly characterized.200919435448
antibacterial profile of fermented seed extracts of ricinus communis: findings from a preliminary analysis.the study was carried out to ascertain the antibacterial properties inherent in fermented seed extracts of ricinus communis. dry seeds of r. communis (castor oil plant) were deshelled, grounded to powder, fermented, and then extracted both with alcohol and water using soxhlet machine. different concentrations of the extracts were tested against selected bacteria using diffusion method of susceptibility testing on sensitivity testing agar medium. klebsiella pneumoniae, escherichia coli, proteus v ...200819434215
genetic requirements for klebsiella pneumoniae-induced liver abscess in an oral infection model.klebsiella pneumoniae is the predominant pathogen of primary liver abscess. however, our knowledge regarding the molecular basis of how k. pneumoniae causes primary infection in the liver is limited. we established an oral infection model that recapitulated the characteristics of liver abscess and conducted a genetic screen to identify the k. pneumoniae genes required for the development of liver abscess in mice. twenty-eight mutants with attenuated growth in liver or spleen samples out of 2,880 ...200919433545
urinary tract infections: bacteriology and antibiotic resistance patterns.the aim of this study was to identify the bacteria causing community acquired urinary tract infections (uti) and detection of antibiotics resistance of isolates in 912 children below 18 years in the west of iran. data were analyzed for 4 age groups: infants, toddlers, preteens and teens. fourteen antibiotics were tested by gel-diffusion method. of 912 patients, 34.2% had positive bacterial cultures. the most common isolates were e. coli (57.4 %), k. pneumoniae (9.7 %), s. aureus (5.8%) and a. ba ...200919430071
performance of the phoenix bacterial identification system compared with disc diffusion methods for identifying extended-spectrum beta-lactamase, ampc and kpc producers.phenotypic identification of ampc, kpc and extended-spectrum beta-lactamases (esbls) among members of the enterobacteriaceae remains challenging. this study compared the phoenix automated microbiology system (bd diagnostics) with the clinical and laboratory standards institute confirmatory method to identify esbl production among 200 escherichia coli and klebsiella pneumoniae clinical isolates. the phoenix system misclassified nearly half of the isolates as esbl-positive, requiring manual testin ...200919429754
determination of emitted airborne microorganisms from a bio-pak wastewater treatment plant.results of a study conducted to assess the degree of airborne microbial contamination generated by a wastewater treatment plant (wwtp) with bioreactor "bio-pak" closed treatment system and evaluation of the dispersion of potential pathogens are described. over the year aerosol samples were collected simultaneously with sedimentation and impact methods from several plant sites and the surroundings. external upwind sites were used as control. total colony-forming counts of heterotrophic psychrophi ...200919428042
dark secrets behind the shimmer of contact lens: the indian scenario.we studied the bacteriological profile of soft contact lens and its accessories among the asymptomatic subjects and monitored the compliance level with the lens use and its cleaning protocol.200919426534
choroidal neovascularaization secondary to klebsiella pneumoniae endogenous abscess and endophthalmitis. 200919426406
a recent evaluation of empirical cephalosporin treatment and antibiotic resistance of changing bacterial profiles in spontaneous bacterial peritonitis.the aim of this research is to evaluate the recent changes in microorganisms causing spontaneous bacterial peritonitis in cirrhotic patients, antibiotic resistance, and response to empirical cephalosporin therapy. a total of 218 patients with ascites secondary to cirrhosis were enrolled. parenteral cefotaxime or cefepime was given to patients who had a neutrophil count of 250/mm(3) or more or a positive bacterial culture of ascitic fluid. antibiotic failure was defined by an absence of clinical ...201019424797
bacterial contamination of patients' medical charts in a surgical ward and the intensive care unit: impact on nosocomial infections.the purpose of this study was to determine the degree of bacterial contamination of patients' files, and to compare the colonized bacteria between files from the surgical intensive care unit (icu) and the surgical ward at the wan fang hospital, taipei, taiwan.200919424563
colonization with cefotazime-resistant enterobacter spp. and klebsiella spp. in hiv-positive cambodian children decreases with immune reconstitution after haart. 200919423482
prevalence of qnra, qnrb, qnrs and aac(6')-ib-cr (in qnr-positive isolates) among esbl-positive and/or ciprofloxacin-resistant isolates in turkey. 200919423478
comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome.we examined a total of 194 patients over 18 years of age with chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities. the following data were obtained for each patient: clinical history--the severity of chronic prostatitis symptoms scored by a croatian translation of the nih cpsi questionnaire, clinical status including digitorectal examination, urethral swab specimens, and selective samples of urine and expressed prostatic secretion, acc ...200919423471
a combination of thalidomide and augmentin protects balb/c mice suffering from klebsiella pneumoniae b5055-induced sepsis.despite extensive research, the mortality associated with sepsis in hospitals remains very high. we have evaluated the protective immunomodulatory effect of thalidomide alone or with augmentin in klebsiella pneumoniae b5055-induced sepsis in balb/c mice. the mouse model of sepsis was developed by placing k. pneumoniae b5055 entrapped in fibrin and thrombin clots in the peritoneal cavity of mice. the septic mice were treated with thalidomide alone (30 mg/kg/day/po), augmentin alone (20 microg/ml/ ...200919423468
comparative in vitro activity of tigecycline against bacteria recovered from clinical specimens in latin america.the present study was performed to evaluate the in vitro activity of tigecycline in comparison to other agents against isolates recovered from patients hospitalized in latin american. organisms were collected in 47 clinical laboratories from 4 countries of latin america between november 2005 and october 2006 and were tested by using disk diffusion method as described by the clsi. a total of 7966 isolates were assessed. tigecycline proved highly active against staphylococci and enterococci (>99% ...200919423466
brain abscess due to klebsiella pneumoniae in a liver-transplanted child.brain abscesses are a rare, severe complication of orthotopic liver transplantation (olt). they are almost exclusively due to fungi, nocardia, or toxoplasma, and usually occur within months of surgery. here we report the case of an adolescent who developed a brain abscess due to klebsiella pneumoniae 11.5 years after olt. fever was absent and laboratory parameters were not indicative of infectious disease, and therefore the diagnosis of a central nervous system neoplasm was considered. subsequen ...200919422671
comparison of chemical compositions and antimicrobial activities of essential oils from three conifer trees; pinus densiflora, cryptomeria japonica, and chamaecyparis obtusa.the chemical compositions, and antibacterial and antifungal effects of essential oils extracted from three coniferous species, pinus densiflora, cryptomeria japonica, and chamaecyparis obtusa, were investigated. gas chromatography mass analysis of the essential oils revealed that the major components and the percentage of each essential oil were 16.66% beta-phellandrene and 14.85% alpha-pinene in p. densiflora; 31.45% kaur-16-ene and 11.06% sabinene in c. japonica; and 18.75% bicyclo [2, 2, 1] h ...200919420996
design, synthesis, antibacterial activity and physicochemical parameters of novel n-4-piperazinyl derivatives of norfloxacin.we report herein the synthesis of some n-mannich bases in addition to different n-4 substituents of norfloxacin. the antibacterial activities of the newly synthesized compounds were evaluated and correlated with their physicochemical properties. results revealed that some of the tested compounds exhibited better inhibitory activities than the reference antibiotic norfloxacin against pseudomonas aeruginosa, escherichia coli, klebsiella pneumonia and staphylococcus aureus strains. correlation resu ...200919419875
[predisposing factors of community acquired bacterial meningitis (excluding neonates)].meningeal defects and primitive ent infections are known to promote pneumococcal meningitis. other risk factors can be identified in the occurrence of community acquired bacterial meningitis (cabm) and play a key role either in the frequency of this kind of infection, the type of bacteria concerned, the prognosis or the risk of recurrence. thus, epidural infiltrations are rarely responsible for staphylococcal or streptococcal meningitis. cochlear implants are also known to increase the risk of p ...200919419829
nosocomial blood stream infection in intensive care units at assiut university hospitals (upper egypt) with special reference to extended spectrum beta-lactamase producing organisms.this study investigated the nosocomial blood stream infection (bsi) in the adult icus in assiut university hospitals to evaluate the rate of infection in different icus, causative microorganisms, antimicrobial resistance, outcome of infection, risk factors, prevalence of extended spectrum b-lactamase producing organisms and molecular typing of klebsiella pneumoniae strains to highlight the role of environment as a potential source of nosocomial bsi.200919419535
[etiology of urinary tract infections and antimicrobial susceptibility: a study conducted on a population of children hospitalized in the department of pediatrics at warsaw bielany hospital; 2004-2006].the basis of urinary tract infection (uti) diagnosis in children is based on positive results of microbiological tests. the selection of an antimicrobial drug for empirical treatment and prophylaxis of uti is determined by the susceptibility of bacterial strains isolated from urine cultures.200819418948
resistance patterns of bacterial isolates to antimicrobials from 3 hospitals in the united arab emirates.to compare the resistance pattern of common bacterial pathogens to commonly used drugs.200919417958
increase in detectable opportunistic bacteria in the oral cavity of orthodontic patients.this study was performed to detect the opportunistic bacteria and fungi from the oral cavities of orthodontic patients and examine the ability of the organisms to adhere to saliva-coated metallic brackets.200919416094
composition and antimicrobial activity of marrubium incanum desr. (lamiaceae) essential oil.the essential oil from the aerial parts of marrubium incanum desr. (lamiaceae), obtained by hydrodistillation, was analyzed by gc and gc-ms. forty-six compounds were identified, representing 96.3% of the total oil. the main components of the oil were (e)-caryophyllene (27.0%), germacrene d (26.2%) and bicyclogermacrene (11.5%). the microbial growth inhibitory properties of the isolated essential oil were determined using the agar diffusion and broth microdilution method against seven bacterial s ...200919413128
prevalence of qnr determinants among extended-spectrum beta-lactamase-positive enterobacteriaceae clinical isolates in southern stockholm, sweden.three hundred and nineteen extended-spectrum beta-lactamase-positive enterobacteriaceae clinical isolates were screened for qnr genes. twelve isolates were positive for qnr, including one qnra1, two qnrb1, three qnrb2, one qnrb4, one qnrb6 and four qnrs1. no qnr-positive strains were identified among the isolates recovered before 2006. the first qnr-positive escherichia coli was detected from a patient in 2006. qnr genes remained rare in e. coli (6/288; 2.1%), but appeared to be more prevalent i ...200919409763
[stability: a factor to consider in antibiotic-lock solutions].antibiotic-lock therapy (alt) has been related to a reduction in the need for catheter withdrawal in patients with catheter-related infection. the stability of the antimicrobial solutions used in alt has not been sufficiently investigated. a systematic literature review was performed to identify articles including studies on the stability of alt solutions. nine studies fulfilled the inclusion criteria requiring specific drug determination techniques, and no apparent drug alterations were observe ...201019409671
can we rely on one laboratory test in detection of extended-spectrum beta-lactamases among enterobacteriaceae? an evaluation of the vitek 2 system and comparison with four other detection methods in kuwait.background and200919406738
paradoxical effect of klebsiella pneumoniae ompk36 porin deficiency.to evaluate the effect of defined mutations in the major ompk35 and ompk36 porins in klebsiella pneumoniae on the activity of two common plasmid-mediated ampc enzymes.200919404854
retrospective analysis of clinical profile prognostic factors and outcomes of 19 patients of emphysematous pyelonephritis.we aimed to study the clinical profile, prognostic factors, and the 6-month outcome of patients with emphysematous pyelonephritis (epn) methods: all patients admitted with a diagnosis of emphysematous pyelonephritis between january 2001 and july 2007 were included.200919404766
pharmacokinetic considerations regarding tigecycline for multidrug-resistant (mdr) klebsiella pneumoniae or mdr acinetobacter baumannii urosepsis. 200919403778
klebsiella pneumoniae carbapenemase, canada. 200919402984
clinical features of neonatal sepsis caused by resistant gram-negative bacteria.clinical features and outcomes of neonatal sepsis caused by resistant gram-negative bacteria are not well described in jordan. the aim of the present study was therefore to describe microbiology and clinical features, laboratory findings and outcomes of early- and late-onset gram-negative neonatal sepsis.200919400829
acute pyelonephritis: clinical characteristics and the role of the surgical treatment.the epidemiology of acute pyelonephritis (apn) has changed with time. therefore we investigated the current clinical characteristics of apn and the significance of proper surgical management for treatment of 1,026 apn patients in south korea for the past 5 yr. the male-to-female ratio was about 1:8. the peak ages of female patients were 20s (21.3%) and over 60s (23.7%), while that of male was over 60s (38.1%). the occurrence of sepsis was 10.1%. complicated apn patients were 35.4%. ninety-four p ...200919399273
high production of 2,3-butanediol from glycerol by klebsiella pneumoniae g31.the microbial production of high amounts of 2,3-butanediol (2,3-bd) from glycerol as a sole carbon source by the bulgarian isolate klebsiella pneumoniae g31 was studied in a series of fed-batch processes. the following conditions were evaluated as optimal: micro-aerobic cultivation in modified media, without ph control. beginning at ph 8, 49.2 g/l of 2,3-bd was produced as negligible concentrations of by-products were received. the ph is the most important factor ruling the 2,3-bd production. sp ...200919396438
[candida-induced recurrent peritonitis after peritoneal catheter reinsertion]. 200919396335
microbial tolerance in secondary peritonitis is dose dependent.local microbial tolerance was investigated in a murine model of peritonitis. peritoneal bacterial burden and inflammatory cytokine concentrations were determined at different times, within 48h after infection. peritoneal macrophages were harvested from naïve mice or from mice 48h after infection and underwent ex vivo stimulation with different concentrations of klebsiella. cytokine secretion was determined in the supernatants. peritoneal bacteria concentrations, remained relatively steady betwee ...200919393606
two chromosomally located qnrb variants, qnrb6 and the new qnrb16, in citrobacter spp. isolates causing bacteraemia.the objective of this study was to determine the prevalence of the plasmid-borne quinolone resistance genes qnra, qnrb and qnrs in a collection of enterobacteriaceae causing bacteraemia. the presence of the three genes was tested for using multiplex pcr in 306 clinical isolates. plasmid analysis was performed using i-ceui and s1 nuclease digestion and hybridization with specific probes for the qnr and 23s rrna genes. five strains were found to carry a qnr gene, one of which, qnrb16, a new varian ...200919392890
correlation between time to positivity of blood cultures with clinical presentation and outcomes in patients with klebsiella pneumoniae bacteraemia: prospective cohort study.time to positivity (ttp) of blood cultures in patients with bacteraemia is considered to be a predictor of outcome for some bacterial species. two hundred and thirty-one patients with klebsiella pneumoniae monomicrobial bacteraemia at a hospital from 1 january to 31 december 2007 were prospectively enrolled. ttp <7 h (46 patients, 19.9%) was associated with a higher pittsburg bacteraemia score (6.2 +/- 5.5 vs. 3.7 +/- 4.3, p 0.002), fewer non-fatal diseases by the mccabe classification (39.1% vs ...200919392886
treatment of strongyloides stercoralis hyperinfection-associated septic shock and acute respiratory distress syndrome with drotrecogin alfa (activated) in a renal transplant recipient.we report a case of strongyloides stercoralis hyperinfection syndrome in a renal transplant recipient complicated by septic shock, acute respiratory distress syndrome, and klebsiella pneumoniae superinfection. the patient was treated successfully with drotrecogin alfa (activated), parenteral ivermectin, albendazole, and piperacillin/tazobactam. this outcome suggests that drotrecogin alfa (activated) may be useful therapy for transplant recipients who develop severe sepsis or septic shock seconda ...200919392733
endoscopic ultrasound-guided aspiration and oral antibiotic therapy as definitive treatment of an asymptomatic pancreatic abscess. 200919390411
prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in korea.the objective of this study was to assess trends in the prevalence and distribution of gram-negative bacteria isolated from bovine mastitis and their antimicrobial susceptibilities during a 6-yr period between 2003 and 2008 in korea. escherichia coli, pseudomonas fluorescens, klebsiella pneumoniae, enterobacter cloacae, acinetobacter lwoffi/junii, pseudomonas aeruginosa, and serratia marcescens were the most commonly observed pathogens during this period. generally, gram-negative bacteria showed ...200919389959
the prevalence of extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae among clinical isolates from a general hospital in iran.this study was conducted at a 900+ bed general teaching hospital, from may to september 2007, in iran. the aim of this study was to determine the prevalence of extended-spectrum beta-lactamase (esbl) producing escherichia coli and klebsiella pneumoniae and their antimicrobial pattern. the kirby-bauer disk diffusion method and the phenotypic disk confirmatory test were performed for each isolate. the total of 206 isolates including 106 e. coli and 100 k. pneumoniae were collected of which 122 iso ...200919388560
field applications of a bio-trickling filter for the removal of nitrogen oxides from flue gas.a bio-trickling filter (btf) packed with polyhedral spheres was used to remove nitrogen oxides (nox) from the flue gas of a coal-fired power plant. the btf system consistently removed 64-95% of the nox after start-up and acclimation under dynamic conditions (e.g., 120-240 m(3)/h flue gas flow rate and inlet 300-900 mg nox/m(3)). scanning electron microscopy of the biofilms that were formed showed a shift in the predominating bacteria. analyses by pcr-denaturing gradient gel electrophoresis showe ...200919387558
a pain in the neck -- an unexpected complication of transrectal ultrasound and biopsy.in the uk, over 26 000 cases of prostate cancer are diagnosed annually, with many more patients undergoing investigation. prostate-specific antigen (psa) testing and its interpretation has always been controversial. many patients undergo psa-driven biopsies, which can cause significant morbidity. we report an unusual but severe complication following transrectal ultrasound and biopsy.200919386955
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