Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| brain-gut microbiome interactions and functional bowel disorders. | alterations in the bidirectional interactions between the intestine and the nervous system have important roles in the pathogenesis of irritable bowel syndrome (ibs). a body of largely preclinical evidence suggests that the gut microbiota can modulate these interactions. a small and poorly defined role for dysbiosis in the development of ibs symptoms has been established through characterization of altered intestinal microbiota in ibs patients and reported improvement of subjective symptoms afte ... | 2014 | 24583088 |
| impact of crispr immunity on the emergence and virulence of bacterial pathogens. | crispr-cas systems protect prokaryotes from viruses and plasmids and function primarily as an adaptive immune system in these organisms. recent discoveries, however, revealed unexpected roles for crispr loci as barriers to horizontal gene transfer and as modulators of gene expression. we review how both of these functions of crispr-cas systems can affect the emergence and virulence of human bacterial pathogens. | 2013 | 24581697 |
| impact of crispr immunity on the emergence and virulence of bacterial pathogens. | crispr-cas systems protect prokaryotes from viruses and plasmids and function primarily as an adaptive immune system in these organisms. recent discoveries, however, revealed unexpected roles for crispr loci as barriers to horizontal gene transfer and as modulators of gene expression. we review how both of these functions of crispr-cas systems can affect the emergence and virulence of human bacterial pathogens. | 2013 | 24581697 |
| health risk assessment for splash parks that use rainwater as source water. | in the netherlands, rainwater becomes more and more popular as an economic and environmentally sustainable water source for splash parks, however, the associated public health risk and underlying risk factors are unknown. since splash parks have been associated with outbreaks of infectious diseases, a quantitative microbial risk assessment was performed using legionella pneumophila as a target pathogen to quantify the risk of infection for exposure due to inhalation and campylobacter jejuni for ... | 2014 | 24576701 |
| disulfide bond formation in prokaryotes: history, diversity and design. | the formation of structural disulfide bonds is essential for the function and stability of a great number of proteins, particularly those that are secreted. there exists a variety of dedicated cellular catalysts and pathways from archaea to humans that ensure the formation of native disulfide bonds. in this review we describe the initial discoveries of these pathways and report progress in recent years in our understanding of the diversity of these pathways in prokaryotes, including those newly ... | 2014 | 24576574 |
| diagnostic metagenomics: potential applications to bacterial, viral and parasitic infections. | the term 'shotgun metagenomics' is applied to the direct sequencing of dna extracted from a sample without culture or target-specific amplification or capture. in diagnostic metagenomics, this approach is applied to clinical samples in the hope of detecting and characterizing pathogens. here, i provide a conceptual overview, before reviewing several recent promising proof-of-principle applications of metagenomics in virus discovery, analysis of outbreaks and detection of pathogens in contemporar ... | 2014 | 24576467 |
| applying the resfinder and virulencefinder web-services for easy identification of acquired antibiotic resistance and e. coli virulence genes in bacteriophage and prophage nucleotide sequences. | extensive research is currently being conducted on the use of bacteriophages for applications in human medicine, agriculture and food manufacturing. however, phages are important vehicles of horisontal gene transfer and play a significant role in bacterial evolution. as a result, concern has been raised that this increased use and dissemination of phages could result in spread of deleterious genes, e.g., antibiotic resistance and virulence genes. meanwhile, in the wake of the genomic era, severa ... | 2014 | 24575358 |
| a comparison between hippurate hydrolysis and multiplex pcr for differentiating campylobacter coli and campylobacter jejuni. | species identification is important for epidemiological, clinical and treatment purposes. the aim of this study was to find out whether hippurate hydrolysis is a reliable test for differentiating between campylobacter coli and campylobacter jejuni. to achieve this, hippurate hydrolysis test was compared with multiplex polymerase chain reaction (mpcr) for their ability to speciate c. coli and c. jejuni. eighteen campylobacter strains from poultry samples were used for this study. the results from ... | 2011 | 24575212 |
| campylobacter concisus and inflammatory bowel disease. | investigation of the possible role of campylobacter concisus (c. concisus) in inflammatory bowel disease (ibd) is an emerging research area. despite the association found between c. concisus and ibd, it has been difficult to explain how c. concisus, a bacterium that is commonly present in the human oral cavity, may contribute to the development of enteric diseases. the evidence presented in this review shows that some c. concisus strains in the oral cavity acquired zonula occludens toxin (zot) g ... | 2014 | 24574800 |
| role of the gut microbiota in inflammatory bowel disease pathogenesis: what have we learnt in the past 10 years? | our understanding of the microbial involvement in inflammatory bowel disease (ibd) pathogenesis has increased exponentially over the past decade. the development of newer molecular tools for the global assessment of the gut microbiome and the identification of nucleotide-binding oligomerization domain-containing protein 2 in 2001 and other susceptibility genes for crohn's disease in particular has led to better understanding of the aetiopathogenesis of ibd. the microbial studies have elaborated ... | 2014 | 24574795 |
| inflammation and colorectal cancer, when microbiota-host mutualism breaks. | structural changes in the gut microbial community have been shown to accompany the progressive development of colorectal cancer. in this review we discuss recent hypotheses on the mechanisms involved in the bacteria-mediated carcinogenesis, as well as the triggering factors favoring the shift of the gut microbiota from a mutualistic to a pro-carcinogenic configuration. the possible role of inflammation, bacterial toxins and toxic microbiota metabolites in colorectal cancer onset is specifically ... | 2014 | 24574765 |
| omics approaches in food safety: fulfilling the promise? | genomics, transcriptomics, and proteomics are rapidly transforming our approaches to the detection, prevention, and treatment of foodborne pathogens. microbial genome sequencing in particular has evolved from a research tool into an approach that can be used to characterize foodborne pathogen isolates as part of routine surveillance systems. genome sequencing efforts will not only improve outbreak detection and source tracking, but will also create large amounts of foodborne pathogen genome sequ ... | 2014 | 24572764 |
| functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (ppx/gppa) of campylobacter jejuni. | the inorganic polyphosphate (poly-p) is a key regulator of stress responses and virulence in many bacterial pathogens including campylobacter jejuni. the role of exopolyphosphatases/guanosine pentaphosphate (pppgpp) phosphohydrolases (ppx/gppa) in poly-p homeostasis and c. jejuni pathobiology remains unexplored. here, we analyzed deletion mutants (∆ppx1, ∆ppx2) and the double knockout mutant (dkppx), all ∆ppx mutants exhibited increased capacity to accumulate poly-p; however only ∆ppx1 and dkppx ... | 2014 | 24569519 |
| a pcr-rflp assay for the detection and differentiation of campylobacter jejuni, c. coli, c. fetus, c. hyointestinalis, c. lari, c. helveticus and c. upsaliensis. | although campylobacter jejuni and campylobacter coli are the most common bacterial causes of human gastrointestinal diseases, other campylobacter species are also involved in human and animal infections. in this study, we developed a cytolethal distending toxin (cdt) gene-based pcr-rflp assay for the detection and differentiation of c. jejuni, c. coli, c. fetus, c. hyointestinalis, c. lari, c. helveticus and c. upsaliensis. previously designed common primers, which can amplify the cdtb gene of c ... | 2014 | 24568882 |
| antimicrobial resistance of bacterial enteropathogens isolated from stools in madagascar. | diarrheal diseases are a major public health problem in developing countries, and are one of the main causes of hospital admissions in madagascar. the pasteur institute of madagascar undertook a study to determine the prevalence and the pathogenicity of bacterial, viral and protozoal enteropathogens in diarrheal and non-diarrheal stools of children aged less than 5 years in madagascar. we present here the results of the analysis of antimicrobial susceptibility of the bacteria isolated during thi ... | 2014 | 24568189 |
| attenuation of intestinal inflammation in interleukin-10-deficient mice infected with citrobacter rodentium. | interleukin-10 (il-10) curtails immune responses to microbial infection and autoantigens and contributes to intestinal immune homeostasis, yet administration of il-10 has not been effective at attenuating chronic intestinal inflammatory conditions, suggesting that its immune functions may be context dependent. to gain a broader understanding of the importance of il-10 in controlling mucosal immune responses to infectious challenges, we employed the murine attaching and effacing pathogen citrobac ... | 2014 | 24566625 |
| corbi: a new r package for biological network alignment and querying. | in the last decade, plenty of biological networks are built from the large scale experimental data produced by the rapidly developing high-throughput techniques as well as literature and other sources. but the huge amount of network data have not been fully utilized due to the limited biological network analysis tools. as a basic and essential bioinformatics method, biological network alignment and querying have been applied in many fields such as predicting new protein-protein interactions (ppi ... | 2013 | 24565104 |
| a base composition analysis of natural patterns for the preprocessing of metagenome sequences. | on the pretext that sequence reads and contigs often exhibit the same kinds of base usage that is also observed in the sequences from which they are derived, we offer a base composition analysis tool. our tool uses these natural patterns to determine relatedness across sequence data. we introduce spectrum sets (sets of motifs) which are permutations of bacterial restriction sites and the base composition analysis framework to measure their proportional content in sequence data. we suggest that t ... | 2013 | 24564274 |
| high frequency, spontaneous mota mutations in campylobacter jejuni strain 81-176. | campylobacter jejuni is an important cause of bacterial diarrhea worldwide. the pathogenesis of c. jejuni is poorly understood and complicated by phase variation of multiple surface structures including lipooligosaccharide, capsule, and flagellum. when c. jejuni strain 81-176 was plated on blood agar for single colonies, the presence of translucent, non-motile colonial variants was noted among the majority of opaque, motile colonies. high-throughput genomic sequencing of two flagellated transluc ... | 2014 | 24558375 |
| gram-negative flagella glycosylation. | protein glycosylation had been considered as an eccentricity of a few bacteria. however, through advances in analytical methods and genome sequencing, it is now established that bacteria possess both n-linked and o-linked glycosylation pathways. both glycosylation pathways can modify multiple proteins, flagellins from archaea and eubacteria being one of these. flagella o-glycosylation has been demonstrated in many polar flagellins from gram-negative bacteria and in only the gram-positive genera ... | 2014 | 24557579 |
| campylobacter infection in chickens modulates the intestinal epithelial barrier function. | asymptomatic carriage of campylobacter jejuni is highly prevalent in chicken flocks. thus, we investigated whether chronic campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. an experiment was carried out in which commercial chickens were orally infected with c. jejuni (1 × 10(8) cfu/bird) at 14 days of life. changes in ion transport and barrier function were assessed by short-circuit current (i(sc)) and transepithelial ion conductance (g(t)) in ... | 2015 | 24553586 |
| comparative proteomic label-free analysis of campylobacter jejuni nctc 11168 cultured with porcine mucin. | campylobacter jejuni is a major gastrointestinal pathogen in humans. poultry is a primary reservoir for c. jejuni, and c. jejuni appears to be highly adapted to the gastrointestinal tracts of avian species. we determined the protein expression profiles of c. jejuni nctc 11168 cultured in medium containing porcine mucin. differentially expressed proteins in the presence and absence of porcine mucin were identified using the label-free method. we identified 52 proteins with expression that was eit ... | 2014 | 24552179 |
| prediction of ctl epitope, in silico modeling and functional analysis of cytolethal distending toxin (cdt) protein of campylobacter jejuni. | campylobacter jejuni is a potent bacterial pathogen culpable for diarrheal disease called campylobacteriosis. it is realized as a major health issue attributable to unavailability of appropriate vaccines and clinical treatment options. as other pathogens, c. jejuni entails host cellular components of an infected individual to disseminate this disease. these host-pathogen interfaces during c. jejuni infection are complex, vibrant and involved in the nicking of host cell environment, enzymes and p ... | 2014 | 24552167 |
| starvation induces phenotypic diversification and convergent evolution in vibrio vulnificus. | starvation is a common stress experienced by bacteria living in natural environments and the ability to adapt to and survive intense stress is of paramount importance for any bacterial population. a series of starvation experiments were conducted using v. vulnificus 93u204 in phosphate-buffered saline and seawater. the starved population entered the death phase during the first week and approximately 1% of cells survived. after that the population entered a long-term stationary phase, and could ... | 2014 | 24551129 |
| antisecretory and antimotility activity of aconitum heterophyllum and its significance in treatment of diarrhea. | the roots of the plant aconitum heterophyllum (eah) are traditionally used for curing hysteria, throat infection, dyspepsia, abdominal pain, diabetes, and diarrhea. therefore, the present study was undertaken to determine the mechanism involved in the anti-diarrheal activity of roots of a. heterophyllum. | 2014 | 24550590 |
| small distances can keep bacteria at bay for days. | transmission of pathogens between spatially separated hosts, i.e., indirect transmission, is a commonly encountered phenomenon important for epidemic pathogen spread. the routes of indirect transmission often remain untraced, making it difficult to develop control strategies. here we used a tailor-made design to study indirect transmission experimentally, using two different zoonotic bacteria in broilers. previous experiments using a single bacterial species yielded a delay in the onset of trans ... | 2014 | 24550476 |
| crystal structure and putative substrate identification for the entamoeba histolytica low molecular weight tyrosine phosphatase. | entamoeba histolytica is a eukaryotic intestinal parasite of humans, and is endemic in developing countries. we have characterized the e. histolytica putative low molecular weight protein tyrosine phosphatase (lmw-ptp). the structure for this amebic tyrosine phosphatase was solved, showing the ligand-induced conformational changes necessary for binding of substrate. in amebae, it was expressed at low but detectable levels as detected by immunoprecipitation followed by immunoblotting. a mutant lm ... | 2014 | 24548880 |
| burden of laboratory-confirmed campylobacter infections in guatemala 2008-2012: results from a facility-based surveillance system. | campylobacteriosis is one of the leading causes of gastroenteritis worldwide. this study describes the epidemiology of laboratory-confirmed campylobacter diarrheal infections in two facility-based surveillance sites in guatemala. | 2013 | 24534336 |
| burden of laboratory-confirmed campylobacter infections in guatemala 2008-2012: results from a facility-based surveillance system. | campylobacteriosis is one of the leading causes of gastroenteritis worldwide. this study describes the epidemiology of laboratory-confirmed campylobacter diarrheal infections in two facility-based surveillance sites in guatemala. | 2013 | 24534336 |
| the evidence for clonal spreading of quinolone resistance with a particular clonal complex of campylobacter jejuni. | campylobacter is the most prevalent cause of bacterial gastroenteritis worldwide and it represents a significant public health risk of increasing severity due to its escalating resistance to clinically important quinolone and macrolide antibiotics. as a zoonotic pathogen campylobacter is transmitted along the food chain and naturally cycles from environmental waters, feedstuff, animals and food to humans. we determined antibiotic resistance profiles, as well as multilocus sequence types and flaa ... | 2014 | 24534165 |
| gtfa and gtfb are both required for protein o-glycosylation in lactobacillus plantarum. | acm2, the major autolysin of lactobacillus plantarum wcfs1, was recently found to be o-glycosylated with n-acetylhexosamine, likely n-acetylglucosamine (glcnac). in this study, we set out to identify the glycosylation machinery by employing a comparative genomics approach to identify gtf1 homologues, which are involved in fimbria-associated protein 1 (fap1) glycosylation in streptococcus parasanguinis. this in silico approach resulted in the identification of 6 candidate l. plantarum wcfs1 genes ... | 2014 | 24532775 |
| reduction of microbiological risk in minced meat by a combination of natural antimicrobials. | responsibility for food safety must be taken through the entire food-production chain, to avoid consumer cross-contamination. the antimicrobial activities of an alpinia katsumadai seed extract and epigallocatechin gallate (egcg), and their combination, were evaluated against individual food-borne pathogenic strains of listeria monocytogenes, escherichia coli and campylobacter jejuni, individually and as a cocktail, in chicken-meat juice and sterile minced meat as food models, and in minced meat ... | 2014 | 24532379 |
| a new subfamily of polyphosphate kinase 2 (class iii ppk2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation. | inorganic polyphosphate (polyp) is a linear polymer of tens to hundreds of phosphate (pi) residues linked by "high-energy" phosphoanhydride bonds as in atp. polyp kinases, responsible for the synthesis and utilization of polyp, are divided into two families (ppk1 and ppk2) due to differences in amino acid sequence and kinetic properties. ppk2 catalyzes preferentially polyp-driven nucleotide phosphorylation (utilization of polyp), which is important for the survival of microbial cells under condi ... | 2014 | 24532069 |
| structure of an unusual s-adenosylmethionine synthetase from campylobacter jejuni. | s-adenosylmethionine (adomet) participates in a wide range of methylation and other group-transfer reactions and also serves as the precursor for two groups of quorum-sensing molecules that function as regulators of the production of virulence factors in gram-negative bacteria. the synthesis of adomet is catalyzed by adomet synthetases (mats), a ubiquitous family of enzymes found in species ranging from microorganisms to mammals. the adomet synthetase from the bacterium campylobacter jejuni (cjm ... | 2014 | 24531478 |
| norspermidine is not a self-produced trigger for biofilm disassembly. | formation of bacillus subtilis biofilms, consisting of cells encapsulated within an extracellular matrix of exopolysaccharide and protein, requires the polyamine spermidine. a recent study reported that (1) related polyamine norspermidine is synthesized by b. subtilis using the equivalent of the vibrio cholerae biosynthetic pathway, (2) exogenous norspermidine at 25 μm prevents b. subtilis biofilm formation, (3) endogenous norspermidine is present in biofilms at 50-80 μm, and (4) norspermidine p ... | 2014 | 24529384 |
| producing proficient methyl donors from alternative substrates of s-adenosylmethionine synthetase. | bacteria use quorum sensing to probe and respond to population densities in their external environment. the detection of quorum signaling molecules causes a virulence response in many pathogenic bacteria. blocking this signaling pathway, without interfering with critical metabolic functions, would produce compounds that can disarm pathogens without killing them. by not blocking growth per se, this therapeutic approach would have a lower associated risk for the development of bacterial resistance ... | 2014 | 24528526 |
| maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar t3ss export. | bacterial swimming is mediated by rotation of a filament that is assembled via polymerization of flagellin monomers after secretion via a dedicated flagellar type iii secretion system. several bacteria decorate their flagellin with sialic acid related sugars that is essential for motility. aeromonas caviae is a model organism for this process as it contains a genetically simple glycosylation system and decorates its flagellin with pseudaminic acid (pse). the link between flagellin glycosylation ... | 2014 | 24527847 |
| comparative genomics of unintrogressed campylobacter coli clades 2 and 3. | campylobacter jejuni and c. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. c. jejuni is scattered into several lineages, with no apparent linkage, whereas c. coli clusters into three distinct phylogenetic groups (clades) of which clade 1 has shown extensive genome-wide introgression with c. jejuni, yet the other two clades (2 and 3) have less than 2% of c. jejuni ancestry. we characterized a c. coli strain (76339) ... | 2014 | 24524824 |
| campylobacter prosthetic joint infection. | a 75-year-old man was diagnosed with probable campylobacter jejuni prosthetic knee infection after a diarrheal illness. joint aspirate and operative cultures were negative, but pcr of prosthesis sonicate fluid was positive, as was stool culture. nineteen additional cases of campylobacter prosthetic joint infection reported in the literature are reviewed. | 2014 | 24523462 |
| recent advances in human milk glycobiology. | 2014 | 24522101 | |
| raw milk consumption among patients with non-outbreak-related enteric infections, minnesota, usa, 2001-2010. | raw milk has frequently been identified as the source of foodborne illness outbreaks; however, the number of illnesses ascertained as part of documented outbreaks likely represents a small proportion of the actual number of illnesses associated with this food product. analysis of routine surveillance data involving illnesses caused by enteric pathogens that were reportable in minnesota during 2001-2010 revealed that 3.7% of patients with sporadic, domestically acquired enteric infections had rep ... | 2014 | 24520559 |
| prevalence of campylobacter among goats and retail goat meat in congo. | the prevalence of campylobacter jejuni and campylobacter coli was determined in goat and goat meat sold at retail outlets in lubumbashi, democratic republic of congo (dr congo). | 2014 | 24518626 |
| what you eat is what you get: novel campylobacter models in the quadrangle relationship between nutrition, obesity, microbiota and susceptibility to infection. | enterocolitis caused by campylobacter jejuni-infections represents an important socioeconomic burden worldwide. recent results from novel murine infection models reveal that the intestinal microbiota is essential for maintaining colonization resistance against c. jejuni. we extended these studies to investigate the role of nutrition and obesity in susceptibility to c. jejuni-infection. gnotobiotic (gb) mice generated by antibiotic treatment, which were fed with a human cafeteria diet (caf), as w ... | 2011 | 24516730 |
| perr controls peroxide- and iron-responsive expression of oxidative stress defense genes in helicobacter hepaticus. | chronic intestinal and hepatic colonization with the microaerophilic murine pathogen helicobacter hepaticus can lead to a range of inflammatory diseases of the lower digestive tract. colonization is associated with an active cellular immune response and production of oxygen radicals. during colonization, h. hepaticus needs to cope with and respond to oxidative stress, and here we report on the role of the h. hepaticus perr-regulator (hh0942) in the expression of the peroxidase-encoding kata (hh0 ... | 2011 | 24516727 |
| comparative variation within the genome of campylobacter jejuni nctc 11168 in human and murine hosts. | campylobacteriosis incited by c. jejuni is a significant enteric disease of human beings. a person working with two reference strains of c. jejuni national collection of type cultures (nctc) 11168 developed symptoms of severe enteritis including bloody diarrhea. the worker was determined to be infected by c. jejuni. in excess of 50 isolates were recovered from the worker's stool. all of the recovered isolates and the two reference strains were indistinguishable from each other based on comparati ... | 2014 | 24516617 |
| detection of cdt toxin genes in campylobacter spp. strains isolated from broiler carcasses and vegetables in são paulo, brazil. | campylobacteriosis is a worldwide distributed zoonosis. one of the main virulence factors related to campylobacter spp. in animals and humans is the cytolethal distending toxin (cdt), encoded by three adjacent genes (cdta, cdtb, cdtc). the occurrence of campylobacter spp. in samples of vegetables has not been reported in brazil yet, and has seldom been described in the international literature. the detection of cdt in these strains has not been reported, either. the objectives of the present stu ... | 2014 | 24516435 |
| detection of cdt toxin genes in campylobacter spp. strains isolated from broiler carcasses and vegetables in são paulo, brazil. | campylobacteriosis is a worldwide distributed zoonosis. one of the main virulence factors related to campylobacter spp. in animals and humans is the cytolethal distending toxin (cdt), encoded by three adjacent genes (cdta, cdtb, cdtc). the occurrence of campylobacter spp. in samples of vegetables has not been reported in brazil yet, and has seldom been described in the international literature. the detection of cdt in these strains has not been reported, either. the objectives of the present stu ... | 2014 | 24516435 |
| the impairment of methylmenaquinol:fumarate reductase affects hydrogen peroxide susceptibility and accumulation in campylobacter jejuni. | the methylmenaquinol:fumarate reductase (mfr) of campylobacter jejuni is a periplasmic respiratory (redox) protein that contributes to the metabolism of fumarate and displays homology to succinate dehydrogenase (sdh). since chemically oxidized redox-enzymes, including fumarate reductase and sdh, contribute to the generation of oxidative stress in escherichia coli, we assessed the role of mfr in c. jejuni after exposure to hydrogen peroxide (h2 o2 ). our results show that a mfr mutant (∆mfra) str ... | 2014 | 24515965 |
| cov2html: a visualization and analysis tool of bacterial next generation sequencing (ngs) data for postgenomics life scientists. | cov2html is an interactive web interface, which is addressed to biologists, and allows performing both coverage visualization and analysis of ngs alignments performed on prokaryotic organisms (bacteria and phages). it combines two processes: a tool that converts the huge ngs mapping or coverage files into light specific coverage files containing information on genetic elements; and a visualization interface allowing a real-time analysis of data with optional integration of statistical results. t ... | 2014 | 24512253 |
| an update in guillain-barré syndrome. | guillain-barré syndrome (gbs) was first described in 1916 (guillain g, 1916) and is approaching its 100th anniversary. our knowledge of the syndrome has hugely expanded since that time. once originally considered to be only demyelinating in pathology we now recognise both axonal and demyelinating subtypes. numerous triggering or antecedent events including infections are recognised and gbs is considered an immunological response to these. gbs is now considered to be a clinical syndrome of an acu ... | 2014 | 24511391 |
| use of synthetic genes for cloning, production and functional expression of the bacteriocins enterocin a and bacteriocin e 50-52 by pichia pastoris and kluyveromyces lactis. | the use of synthetic genes may constitute a successful approach for the heterologous production and functional expression of bacterial antimicrobial peptides (bacteriocins) by recombinant yeasts. in this work, synthetic genes with adapted codon usage designed from the mature amino acid sequence of the bacteriocin enterocin a (enta), produced by enterococcus faecium t136, and the mature bacteriocin e 50-52 (bace50-52), produced by e. faecium nrrl b-32746, were synthesized. the synthetic enta and ... | 2014 | 24510220 |
| evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of shiga toxin-producing escherichia coli in produce. | shiga toxin-producing escherichia coli (stec) strains are a leading cause of produce-associated outbreaks in the united states. rapid, reliable, and robust detection methods are needed to better ensure produce safety. we recently developed a loop-mediated isothermal amplification (lamp) suite for stec detection. in this study, the stec lamp suite was comprehensively evaluated against real-time quantitative pcr (qpcr) using a large panel of bacterial strains (n = 156) and various produce items (s ... | 2014 | 24509927 |
| mycobacterium tuberculosis cyclophilin a uses novel signal sequence for secretion and mimics eukaryotic cyclophilins for interaction with host protein repertoire. | cyclophilins are prolyl isomerases with multitude of functions in different cellular processes and pathological conditions. cyclophilin a (ppia) of mycobacterium tuberculosis is secreted during infection in intraphagosomal niche. however, our understanding about the evolutionary origin, secretory mechanism or the interactome of m. tuberculosis ppia is limited. this study demonstrates through phylogenetic and structural analyses that ppia has more proximity to human cyclophilins than the prokaryo ... | 2014 | 24505389 |
| genome sequences of campylobacter jejuni 81-176 variants with enhanced fitness relative to the parental strain in the chicken gastrointestinal tract. | campylobacter jejuni is a major cause of food-borne infections in the united states due to its ability to asymptomatically colonize the gastrointestinal tracts of chickens. using competition assays with parental c. jejuni 81-176, variants with consistently improved fitness in chicken ceca relative to the parental strain were identified and sequenced. | 2014 | 24503981 |
| mining locus tags in pubmed central to improve microbial gene annotation. | the scientific literature contains millions of microbial gene identifiers within the full text and tables, but these annotations rarely get incorporated into public sequence databases. we propose to utilize the open access (oa) subset of pubmed central (pmc) as a gene annotation database and have developed an r package called pmcxml to automatically mine and extract locus tags from full text, tables and supplements. | 2014 | 24499370 |
| role of alkyl hydroperoxide reductase (ahpc) in the biofilm formation of campylobacter jejuni. | biofilm formation of campylobacter jejuni, a major cause of human gastroenteritis, contributes to the survival of this pathogenic bacterium in different environmental niches; however, molecular mechanisms for its biofilm formation have not been fully understood yet. in this study, the role of oxidative stress resistance in biofilm formation was investigated using mutants defective in catalase (kata), superoxide dismutase (sodb), and alkyl hydroperoxide reductase (ahpc). biofilm formation was sub ... | 2014 | 24498070 |
| biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ... | 2014 | 24498018 |
| [microbiological diagnosis of infections caused by campylobacter jejuni and campylobacter coli in humans]. | campylobacter jejuni and campylobacter coli are gram-negative, microaerophilic bacteria which are worldwide in distribution, causing a zoonotic disease in humans called campylobacteriosis. these infections are mainly caused by eating contaminated food products, most often improperly prepared poultry meat. campylobacteriosis usually takes the form of gastroenteritis, or inflammation of the intestines, and the characteristic symptoms are watery-mucous diarrhea often with the presence of blood in s ... | 2014 | 24491895 |
| long-term alteration of intestinal microbiota in patients with ulcerative colitis by antibiotic combination therapy. | previous work has demonstrated that intestinal bacteria, such as fusobacterium varium (f. varium), contribute to the clinical activity in ulcerative colitis (uc); thus, an antibiotic combination therapy (amoxicillin, tetracycline, and metronidazole (atm)) against f. varium can induce and maintain uc remission. therefore, we investigated whether atm therapy induces a long-term alteration of intestinal microbiota in patients with uc. patients with uc were enrolled in a multicenter, randomized, dou ... | 2014 | 24489770 |
| natural competence and the evolution of dna uptake specificity. | many bacteria are naturally competent, able to actively transport environmental dna fragments across their cell envelope and into their cytoplasm. because incoming dna fragments can recombine with and replace homologous segments of the chromosome, competence provides cells with a potent mechanism of horizontal gene transfer as well as access to the nutrients in extracellular dna. this review starts with an introductory overview of competence and continues with a detailed consideration of the dna ... | 2014 | 24488316 |
| distribution of indigenous bacterial pathogens and potential pathogens associated with roof-harvested rainwater. | the harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as australia, germany, and south africa, among others. however, conflicting reports on the microbial quality of harvested rainwater have been published. to monitor the presence of potential pathogenic bacteria during high-rainfall periods, rainwater from 29 rainwater tanks was sampled on four occasions (during june and august 2012) in a sustainable housing project in kleinmond, south africa. t ... | 2014 | 24487540 |
| comparative analysis and limitations of ethidium monoazide and propidium monoazide treatments for the differentiation of viable and nonviable campylobacter cells. | the lack of differentiation between viable and nonviable bacterial cells limits the implementation of pcr-based methods for routine diagnostic approaches. recently, the combination of a quantitative real-time pcr (qpcr) and ethidium monoazide (ema) or propidium monoazide (pma) pretreatment has been described to circumvent this disadvantage. in regard to the suitability of this approach for campylobacter spp., conflicting results have been reported. thus, we compared the suitabilities of ema and ... | 2014 | 24487529 |
| proteomic identification of immunodominant membrane-related antigens in campylobacter jejuni associated with sheep abortion. | campylobacter jejuni clone sa is the predominant agent inducing sheep abortion and a zoonotic agent causing gastroenteritis in humans in the united states. in an attempt to identify antigens of clone sa that may be useful for vaccine development, immunoproteomic analyses were conducted to characterize the membrane proteome of c. jejuni clone sa. 2-de of c. jejuni membrane-related proteins was followed by immunoblotting analyses using convalescent sera that were derived from ewes naturally infect ... | 2014 | 24487037 |
| compositional and functional features of the gastrointestinal microbiome and their effects on human health. | the human gastrointestinal tract contains distinct microbial communities that differ in composition and function based on their location, as well as age, sex, race/ethnicity, and diet of their host. we describe the bacterial taxa present in different locations of the gi tract, and their specific metabolic features. the distinct features of these specific microbial communities might affect human health and disease. several bacterial taxa and metabolic modules (biochemical functions) have been ass ... | 2014 | 24486050 |
| s-layers: principles and applications. | monomolecular arrays of protein or glycoprotein subunits forming surface layers (s-layers) are one of the most commonly observed prokaryotic cell envelope components. s-layers are generally the most abundantly expressed proteins, have been observed in species of nearly every taxonomical group of walled bacteria, and represent an almost universal feature of archaeal envelopes. the isoporous lattices completely covering the cell surface provide organisms with various selection advantages including ... | 2014 | 24483139 |
| first complete genome sequence of escherichia albertii strain kf1, a new potential human enteric pathogen. | escherichia albertii has been recently recognized as an emerging human and bird enteric pathogen. here, we report the first complete chromosome nucleotide sequence of a clinical isolate of e. albertii strain kf1, which may provide information about the pathogenic potential of this new species and the mechanisms of evolution of enteropathogenic escherichia spp. | 2014 | 24482506 |
| synthesis and structure-activity relationship studies of n-terminal analogues of the antimicrobial peptide tridecaptin a(1). | chemical synthesis was used to increase the potency of the antimicrobial lipopeptide tridecaptin a1. lipid tail modification proved to be an ideal platform for synthesizing structurally simpler analogues that are not readily accessible by isolation. the stereochemical elements of the tridecaptin a1 lipid tail are not essential for antimicrobial activity and could be replaced with hydrophobic aliphatic or aromatic groups. some simpler analogues displayed potent antimicrobial activity against gram ... | 2014 | 24479847 |
| guillain-barré syndrome in pregnancy: an unusual case. | guillain-barré syndrome (gbs) is rare in pregnancy with an estimated incidence between 1.2 and 1.9 cases per 100,000 people annually, and it carries a high maternal risk. we report a 29-year-old primigravida who had pain and progressive heaviness of both lower limbs in her third trimester of pregnancy. the attending gynecologist ascribed these symptoms to ongoing pregnancy. the intrapartum period (lower segment caesarian section) passed uneventfully. on third postpartum day, the patient develope ... | 2013 | 24479054 |
| transition metal ions at the crossroads of mucosal immunity and microbial pathogenesis. | transition metal ions are essential micronutrients for all living organisms. in mammals, these ions are often protein-bound and sequestered within cells, limiting their availability to microbes. moreover, in response to infection, mammalian hosts further reduce the availability of metal nutrients by activating epithelial cells and recruiting neutrophils, both of which release metal-binding proteins with antimicrobial function. microorganisms, in turn, have evolved sophisticated systems to overco ... | 2014 | 24478990 |
| claudins in intestines: distribution and functional significance in health and diseases. | intestines are organs that not only digest food and absorb nutrients, but also provide a defense barrier against pathogens and noxious agents ingested. tight junctions (tjs) are the most apical component of the junctional complex, providing one form of cell-cell adhesion in enterocytes and playing a critical role in regulating paracellular barrier permeability. alteration of tjs leads to a number of pathophysiological diseases causing malabsorption of nutrition and intestinal structure disruptio ... | 2013 | 24478939 |
| potential of known and short prokaryotic protein motifs as a basis for novel peptide-based antibacterial therapeutics: a computational survey. | short linear motifs (slims) are functional stretches of protein sequence that are of crucial importance for numerous biological processes by mediating protein-protein interactions. these motifs often comprise peptides of less than 10 amino acids that modulate protein-protein interactions. while well-characterized in eukaryotic intracellular signaling, their role in prokaryotic signaling is less well-understood. we surveyed the distribution of known motifs in prokaryotic extracellular and virulen ... | 2014 | 24478765 |
| comparison of conventional pcr, multiplex pcr, and loop-mediated isothermal amplification assays for rapid detection of arcobacter species. | this study aimed to develop a loop-mediated isothermal amplification (lamp) method for the rapid detection of arcobacter species. specific primers targeting the 23s ribosomal rna gene were used to detect arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. the specificity of the lamp primer set was assessed using dna samples from a panel of arcobacter and campylobacter species, and the sensitivity was determined using serial dilutions of arcobacter species cultures. lamp show ... | 2014 | 24478488 |
| pathogens of bovine respiratory disease in north american feedlots conferring multidrug resistance via integrative conjugative elements. | in this study, we determined the prevalence of bovine respiratory disease (brd)-associated viral and bacterial pathogens in cattle and characterized the genetic profiles, antimicrobial susceptibilities, and nature of antimicrobial resistance determinants in collected bacteria. nasopharyngeal swab and lung tissue samples from 68 brd mortalities in alberta, canada (n = 42), texas (n = 6), and nebraska (n = 20) were screened using pcr for bovine viral diarrhea virus (bvdv), bovine respiratory syncy ... | 2014 | 24478472 |
| simultaneous detection of five enteric viruses associated with gastroenteritis by use of a pcr assay: a single real-time multiplex reaction and its clinical application. | we developed a highly sensitive reverse transcription and multiplex real-time pcr (rtpcr) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups i and ii; sapovirus genogroups i, ii, iv, and v; human rotavirus a; adenovirus serotypes 40 and 41; and human astrovirus. in comparison to monoplex rtpcr assays, the sensitivities and specificities of the multiplex rtpcr ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clini ... | 2014 | 24478418 |
| radical s-adenosylmethionine enzymes. | 2014 | 24476342 | |
| helicobacter hepaticus, a new pathogenic species of the helicobacter genus: similarities and differences with h. pylori. | helicobacter hepaticus was discovered in 1992 as a cause of liver cancer in the a/jcr mouse model. in susceptible mice, infection by h. hepaticus causes chronic gastrointestinal inflammation leading to neoplasia. it can also cause morphological changes in breast-glands leading to neoplasm and adenocarcinoma in mouse models. studies performed on humans have revealed that h. hepaticus may also be a human pathogen since infection by h. hepaticus can be associated with cholecystitis, cholelithiasis ... | 2013 | 24475322 |
| a variable homopolymeric g-repeat defines small rna-mediated posttranscriptional regulation of a chemotaxis receptor in helicobacter pylori. | phase variation of hypermutable simple sequence repeats (ssrs) is a widespread and stochastic mechanism to generate phenotypic variation within a population and thereby contributes to host adaptation of bacterial pathogens. although several examples of ssrs that affect transcription or coding potential have been reported, we now show that a ssr also impacts small rna-mediated posttranscriptional regulation. based on in vitro and in vivo analyses, we demonstrate that a variable homopolymeric g-re ... | 2014 | 24474799 |
| rapid diagnosis of campylobacter jejuni by stool gram stain examination. | 2014 | 24473432 | |
| helicobacter pylori: expect the unexpected. | helicobacter pylori is one of the most common bacterial infections worldwide, and virtually all infected persons develop coexisting gastritis, a signature feature of which is the capacity to persist for decades. in support of its lifestyle, h. pylori has evolved to express an array of diverse phenotypes, including enzyme functional diversity, that help to subvert obstacles presented by the human host, which permits long-term microbial colonization. the versatility of the newly discovered enzyme ... | 2014 | 24471732 |
| bickerstaff brainstem encephalitis in pediatrics - a case report. | 2013 | 24470835 | |
| is the campylobacter jejuni secretory protein cj0069 a suitable antigen for serodiagnostics? | campylobacter spp. is the most common bacterial pathogen of gastroenteritis worldwide. poultry is the main reservoir and consequently the main origin of infections for humans. as a consequence of a primary campylobacter infection which typically manifests as diarrhea, there is an increased risk to suffer from post-infectious complications such as reactive arthritis, neuropathia, myositis or a guillain-barré syndrome. usually the verification of acute campylobacteriosis is made by stool culture. ... | 2011 | 24466437 |
| pre- and neonatal exposure to lipopolysaccharide or the enteric metabolite, propionic acid, alters development and behavior in adolescent rats in a sexually dimorphic manner. | alterations in the composition of the gut microbiome and/or immune system function may have a role in the development of autism spectrum disorders (asd). the current study examined the effects of prenatal and early life administration of lipopolysaccharide (lps), a bacterial mimetic, and the short chain fatty acid, propionic acid (ppa), a metabolic fermentation product of enteric bacteria, on developmental milestones, locomotor activity, and anxiety-like behavior in adolescent male and female of ... | 2014 | 24466331 |
| mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus. | dietary composition largely influences pig's gastrointestinal microbiota and represents a useful prophylactic tool against enteric disturbances in young pigs. despite the importance for host-microbe interactions and bacterial colonization, dietary responses of the mucosa-associated bacterial communities are less well investigated. in the present study, we characterized the mucosa-associated bacterial communities at the pars non-glandularis of the stomach, ileum and colon, and identified shifts i ... | 2014 | 24466298 |
| presence and analysis of plasmids in human and animal associated arcobacter species. | in this study, we report the screening of four arcobacter species for the presence of small and large plasmids. plasmids were present in 9.9% of the 273 examined strains. one arcobacter cryaerophilus and four arcobacter butzleri plasmids were selected for further sequencing. the size of three small plasmids isolated from a. butzleri and the one from a. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from a. butzleri, was 27.4 kbp. the g+c content ... | 2014 | 24465575 |
| refined nrfa phylogeny improves pcr-based nrfa gene detection. | dissimilatory nitrate reduction to ammonium (dnra) and denitrification are contrasting microbial processes in the terrestrial nitrogen (n) cycle, in that the former promotes n retention and the latter leads to n loss (i.e., the formation of gaseous products). the nitrite reductase nrfa catalyzes nitrite reduction to ammonium, the enzyme associated with respiratory nitrite ammonification and the key step in dnra. although well studied biochemically, the diversity and phylogeny of this enzyme had ... | 2014 | 24463965 |
| neurological aspects of human parvovirus b19 infection: a systematic review. | parvovirus b19 has been linked with various clinical syndromes including neurological manifestations. however, its role in the latter remains not completely understood. although the last 10 years witnessed a surge of case reports on b19-associated neurological aspects, the literature data remains scattered and heterogeneous, and epidemiological information on the incidence of b19-associated neurological aspects cannot be accurately extrapolated. the aim of this review is to identify the characte ... | 2014 | 24459081 |
| complete posttranslational modification mapping of pathogenic neisseria meningitidis pilins requires top-down mass spectrometry. | in pathogenic bacteria, posttranslationally modified proteins have been found to promote bacterial survival, replication, and evasion from the host immune system. in the human pathogen neisseria meningitidis, the protein pile (15-18 kda) is the major building block of type iv pili, extracellular filamentous organelles that play a major role in mediating pathogenesis. previous reports have shown that pile can be expressed as a number of different proteoforms, each harboring its own set of ptms an ... | 2014 | 24459079 |
| manipulation of intestinal epithelial cell function by the cell contact-dependent type iii secretion systems of vibrio parahaemolyticus. | vibrio parahaemolyticus elicits gastroenteritis by deploying type iii secretion systems (ttss) to deliver effector proteins into epithelial cells of the human intestinal tract. the bacteria must adhere to the human cells to allow colonization and operation of the ttss translocation apparatus bridging the bacterium and the host cell. this article first reviews recent advances in identifying the molecules responsible for intercellular adherence. v. parahaemolyticus possesses two ttss, each of whic ... | 2013 | 24455490 |
| manipulation of intestinal epithelial cell function by the cell contact-dependent type iii secretion systems of vibrio parahaemolyticus. | vibrio parahaemolyticus elicits gastroenteritis by deploying type iii secretion systems (ttss) to deliver effector proteins into epithelial cells of the human intestinal tract. the bacteria must adhere to the human cells to allow colonization and operation of the ttss translocation apparatus bridging the bacterium and the host cell. this article first reviews recent advances in identifying the molecules responsible for intercellular adherence. v. parahaemolyticus possesses two ttss, each of whic ... | 2013 | 24455490 |
| single cell stochastic regulation of pilus phase variation by an attenuation-like mechanism. | the molecular triggers leading to virulence of a number of human-adapted commensal bacteria such as streptococcus gallolyticus are largely unknown. this opportunistic pathogen is responsible for endocarditis in the elderly and associated with colorectal cancer. colonization of damaged host tissues with exposed collagen, such as cardiac valves and pre-cancerous polyps, is mediated by appendages referred to as pil1 pili. populations of s. gallolyticus are heterogeneous with the majority of cells w ... | 2014 | 24453966 |
| incorporation of phosphorylcholine into the lipooligosaccharide of nontypeable haemophilus influenzae does not correlate with the level of biofilm formation in vitro. | nontypeable haemophilus influenzae (nthi) is an opportunistic pathogen that causes otitis media in children and community-acquired pneumonia or exacerbations of chronic obstructive pulmonary disease in adults. a large variety of studies suggest that biofilm formation by nthi may be an important step in the pathogenesis of this bacterium. the objective of this report was to determine the relationship between the presence of phosphorylcholine in the lipooligosaccharide of nthi and the level of bio ... | 2014 | 24452688 |
| detection of campylobacter in stool and determination of significance by culture, enzyme immunoassay, and pcr in developing countries. | campylobacter is a common bacterial enteropathogen that can be detected in stool by culture, enzyme immunoassay (eia), or pcr. we compared culture for c. jejuni/c. coli, eia (prospect), and duplex pcr to distinguish campylobacter jejuni/c. coli and non-jejuni/coli campylobacter on 432 diarrheal and matched control stool samples from infants in a multisite longitudinal study of enteric infections in tanzania, bangladesh, and peru. the sensitivity and specificity of culture were 8.5% and 97.6%, re ... | 2014 | 24452175 |
| a novel o-linked glycan modulates campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization. | campylobacter jejuni is an important cause of human foodborne gastroenteritis; strategies to prevent infection are hampered by a poor understanding of the complex interactions between host and pathogen. previous work showed that c. jejuni could bind human histo-blood group antigens (bgags) in vitro and that bgags could inhibit the binding of c. jejuni to human intestinal mucosa ex vivo. here, the major flagella subunit protein (flaa) and the major outer membrane protein (momp) were identified as ... | 2014 | 24451549 |
| flagellin a toll-like receptor 5 agonist as an adjuvant in chicken vaccines. | chicken raised under commercial conditions are vulnerable to environmental exposure to a number of pathogens. therefore, regular vaccination of the flock is an absolute requirement to prevent the occurrence of infectious diseases. to combat infectious diseases, vaccines require inclusion of effective adjuvants that promote enhanced protection and do not cause any undesired adverse reaction when administered to birds along with the vaccine. with this perspective in mind, there is an increased nee ... | 2014 | 24451328 |
| co-occurrence of anaerobic bacteria in colorectal carcinomas. | numerous cancers have been linked to microorganisms. given that colorectal cancer is a leading cause of cancer deaths and the colon is continuously exposed to a high diversity of microbes, the relationship between gut mucosal microbiome and colorectal cancer needs to be explored. metagenomic studies have shown an association between fusobacterium species and colorectal carcinoma. here, we have extended these studies with deeper sequencing of a much larger number (n = 130) of colorectal carcinoma ... | 2013 | 24450771 |
| twin-arginine translocation system in helicobacter pylori: tatc, but not tatb, is essential for viability. | the twin-arginine translocation (tat) system, needed to transport folded proteins across biological membranes, has not been characterized in the gastric pathogen helicobacter pylori. analysis of all h. pylori genome sequences available thus far reveals the presence of single copies of tata, tatb, and tatc needed for the synthesis of a fully functional tat system. based on the presence of the twin-arginine hallmark in their signal sequence, only four h. pylori proteins appear to be tat dependent: ... | 2014 | 24449753 |
| characterization and antigenicity of recombinant campylobacter jejuni flagellar capping protein flid. | campylobacter jejuni, a flagellated, spiral-rod, gram-negative bacterium, is the leading pathogen of human acute bacterial gastroenteritis worldwide, and chickens are regarded as a major reservoir of this micro-organism. bacterial flagella, composed of more than 35 proteins, play important roles in colonization and adhesion to the mucosal surface of chicken caeca. in this study, the flagellar capping protein, flid, encoded by the flid gene, from the campylobacter jenuni d1-39 isolate was express ... | 2014 | 24445509 |
| helicobacter pylori rna polymerase α-subunit c-terminal domain shows features unique to ɛ-proteobacteria and binds nikr/dna complexes. | bacterial rna polymerase is a large, multi-subunit enzyme responsible for transcription of genomic information. the c-terminal domain of the α subunit of rna polymerase (αctd) functions as a dna and protein recognition element localizing the polymerase on certain promoter sequences and is essential in all bacteria. although αctd is part of rna polymerase, it is thought to have once been a separate transcription factor, and its primary role is the recruitment of rna polymerase to various promoter ... | 2014 | 24442709 |
| on the fiftieth anniversary. postinfectious irritable bowel syndrome: mechanisms related to pathogens. | gastrointestinal (gi) infections resulting from bacterial, viral, and parasitic pathogens predispose to postinfectious irritable bowel syndrome (pi-ibs) and other functional gi disorders. existing literature supports the role of enterochromaffin cell hyperplasia, serotonin synthesis and reuptake, impaired barrier function, altered immune activation, and potentially mast cell activation in the pathophysiology of pi-ibs. | 2014 | 24438587 |
| campylobacter coli cultured from the stools of a patient with immunoproliferative small intestinal disease. | campylobacter has been associated with immunoproliferative small intestinal disease (ipsid), on the basis of 16s rdna sequencing, in situ hybridization, and immunohistochemistry. here, for the first time, we have cultured campylobacter from the stools of a patient with ipsid. phenotypic analysis and whole genome sequencing identified campylobacter coli. pcr on a ipsid tissue biopsy sample was positive for campylobacter coli and negative for campylobacter jejuni. these findings further support a ... | 2014 | 24438451 |