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interactions of butyric acid- and acetic acid-treated salmonella with chicken primary cecal epithelial cells in vitro.in vitro studies of the interaction between pathogenic bacteria and the chicken intestinal epithelium are hampered by the lack of a host- and tissue-specific in vitro model. therefore, a reproducible method for isolation and cultivation of chicken primary cecal epithelial cells was developed. cecal crypts were isolated and cultured in vitro to form a semiconfluent layer of epithelial cells. incubation of salmonella enteritidis with these cells resulted in invasion. pretreatment of the salmonella ...200415283426
improved efficacy of whole genome amplification from bacterial cells. 200415283198
effect of sunlight on the survival of salmonella on surfaces.to investigate the effect of simulated full-spectrum tropical sunlight on the survival of salmonella in droplets on surfaces.200415281943
mesenteric adenitis caused by salmonella enterica serovar enteritidis.mesenteric adenitis is a self-limited condition characterized by fever, localized right lower quadrant abdominal pain, and frequent leukocytosis, making it difficult to differentiate from appendicitis. we report a case of mesenteric adenitis in an 8-year-old boy who presented at the emergency department with right lower quadrant abdominal pain, diarrhea, and fever up to 40 degrees c. acute appendicitis was initially suspected, but further abdominal ultrasound and contrast enhanced computed tomog ...200415278192
observations on salmonella contamination of eggs from infected commercial laying flocks where vaccination for salmonella enterica serovar enteritidis had been used.eggs were collected monthly from 12 cage-layer flocks on four farms where salmonella enteritidis was present in vaccinated flocks despite vaccination with an s. enteritidis bacterin. where possible, hens were also taken for culture at the end of the laying period, and faecal and environmental samples were taken from the laying houses before and after cleaning and disinfection. twenty-four batches of six egg shells from the 13 652 tested (0.18% [0.11 to 0.26 ci(95)] single egg equivalent) were po ...200415276979
survival of escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes in kimchi.the survival of gram-positive and gram-negative foodborne pathogens in both commercial and laboratory-prepared kimchi (a traditional fermented food widely consumed in japan) was investigated. it was found that escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes could survive in both commercial and laboratory-prepared kimchi inoculated with these pathogens and incubated at 10 degrees c for 7 days. however, when incubation was prolonged, the s. aureu ...200415270509
prevalence of high-risk egg-preparation practices in restaurants that prepare breakfast egg entrées: an ehs-net study.salmonella enterica serotype enteritidis (se) is a common cause of foodborne illness in the united states. foods prepared with raw shell eggs have often been associated with se outbreaks. the federal government published the egg safety action plan in december 1999 that called for reduction of egg-preparation practices that may contribute to the survival and proliferation of se. in seven states, an interview and brief site evaluation of 153 restaurants that prepare eggs during all hours of operat ...200415270499
validation of time and temperature values as critical limits for salmonella and background flora growth during the production of fresh ground and boneless pork products.to provide pork processors with valuable data to validate the critical limits set for temperature during pork fabrication and grinding, a study was conducted to determine the growth of salmonella serotypes and background flora at various temperatures. growth of salmonella typhimurium and salmonella enteritidis and of background flora was monitored in ground pork and boneless pork chops held at various temperatures to determine growth patterns. case-ready modified atmosphere packaged ground pork ...200415270491
detection and characterization of enterocins from enterococcus sp.examination of 90 isolates of enterococcus sp. revealed production of enterocin by two isolates of e. faecalis which was inhibitory to listeria monocytogenes. two isolates of e. gallinarum produced enterocin active against staphylococcus aureus. none of the isolates antagonized salmonella enteritidis. the enterocins of e. faecalis isolates were inactivated by alpha-chymotrypsin but not by trypsin and papain, while those of e. gallinarum were resistant to all the three enzymes. enterocins produce ...200315266908
enhanced intracellular replication of salmonella enteritidis in hla-b27-expressing human monocytic cells: dependency on glutamic acid at position 45 in the b pocket of hla-b27.to reveal the cause of the impaired elimination of salmonella enteritidis in hla-b27-transfected human monocytic cells and to study whether the b pocket of hla-b27 contributes to these modulatory effects.200415248225
selection of lactobacilli for chicken probiotic adjuncts.during inhibitory activity screening of 296 strains of lactic acid bacteria from the gastro-intestinal tract of chicks, 77 strains showed inhibition against enteric indicator strains (salmonella enteritidis and escherichia coli). eight different strains identified as lactobacillus salivarius were selected for the following attributes: their ability to inhibit all the indicator strains; a high adhesion efficiency to the epithelial cells of chickens and also their resistance to a number of antibio ...199815244067
mutant prevention concentrations of ciprofloxacin and enrofloxacin for salmonella enterica.to determine the mutant prevention concentrations (mpcs) of ciprofloxacin and enrofloxacin against four strains of salmonella enterica serovar enteritidis and four strains of s. typhimurium including one fully susceptible, one multiply resistant (mar), one gyra mutant and one gyra/mar mutant. further, to examine mutants arising after exposure to sub-mpc concentrations of the antibiotics for susceptibility to ciprofloxacin and enrofloxacin, and cyclohexane tolerance.200415243029
colonization of the chicken reproductive tract and egg contamination by salmonella. 200415239689
in vitro effect of c2-c18 fatty acids on salmonellas.the susceptibility of salmonella spp. to 15 fatty acids was determined in vitro in cultures grown on glucose. antimicrobial activity was expressed as ic50 (a concentration at which only 50% of the initial glucose in cultures was utilized). caprylic acid was the only acid inhibiting glucose utilization. in cultures of s. enteritidis, s. infantis and s. typhimurium, ic50 of caprylic acid ranged from 0.75 to 1.17 mg/ml. a moderate adaptation effect was observed as these values increased 1.5-1.8 tim ...200415227798
effect of type 1 fimbriae of salmonella enterica serotype enteritidis on bacteraemia and reproductive tract infection in laying hens.research on the role of type 1 fimbriae of salmonella enterica serotype enteritidis in poultry to date has been focused on the intestinal phase of the infection. this study aimed to investigate the role of type 1 fimbriae in a systemic infection by intravenously inoculating chickens with a fimd mutant or its parent strain. the fimd mutant was present in the blood for 3 weeks after infection, while the wild type parent strain was cleared within the first 3 days. the fimd mutant was isolated at le ...200415223560
in vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens.the purpose of this study was to examine the effectiveness of selected essential oils for the control of growth and survival of pathogenic microorganisms of significant importance in food hygiene and to determine whether the antimicrobial effect was due to the major compounds of the oils. mic and mbc were determined by the tube dilution method. essential oils from thymus vulgaris from spain and france, salvia sclarea, salvia officinalis, salvia lavandulifolia, lavandula latifolia, lavandula angu ...200415222560
serological characterization and prevalence of spvr genes in salmonella isolated from foods involved in outbreaks in brazil.salmonella strains (n = 75) isolated from foods involved in foodborne outbreaks occurred in rio grande do sul state, brazil, during 1999 and 2000 were studied. strains were serotyped and submitted to pcr analysis to verify the prevalence of salmonella plasmid virulence (spvr) regulatory gene. among the 75 isolates, 73 (97%) were classified as salmonella enterica serovar enteritidis. all of the salmonella strains isolated in 1999 were classified as serotype enteritidis, whereas in 2000 two isolat ...200415222555
the effect of surface type and suspending medium on the persistence of salmonella enteritidis pt4. 200415222364
[salmonellosis in poland in 2002].total number of salmonellosis cases slightly increased. in 2002, 20,688 cases were reported (19,881 in previous year), incidence rate = 54.1 per 100,000 population. 68% of patients were hospitalized, but percentage of hospitalized cases with extraintestinal manifestations was much higher--above 91%. the seasonal peak was observed as in previous year in july and august. since 1995 (when salmonella strains were isolated in 91% patients) decreasing trend in confirmation of clinical diagnosis is obs ...200415218645
mycotic aneurysm of the abdominal aorta with retroperitoneal abscess: successful endovascular repair.mycotic aortic aneurysms are rare. improved diagnostic procedures, appropriate antibiotic treatment, and safe surgical techniques have reduced the high mortality associated with bacterial aortitis. however, definite evidence-based conclusions with regard to the surgical strategy cannot be drawn from the data available in the published literature. we report successful endovascular repair of a mycotic abdominal aortic aneurysm. endovascular treatment may offer a benefit, especially in critically i ...200415218478
[bacterial aortitis].a cohort of 14 patients with bacterial destruction of various segments of the aortic wall is presented. the salmonella enteritidis strain was predominantly responsible. most patients had typical history of symptomatic trias of sepsis, abdominal and/or back pain and positive blood cultures. ct scan showed pseudoaneurysm within the thoracic, subphrenic or subrenal aorta as well as acute hemorrhage in three patients. one of these was excluded from invasive treatment due to hopeless prognosis. in on ...200415216674
single-nucleotide polymorphism mutation spectra and resistance to quinolones in salmonella enterica serovar enteritidis with a mutator phenotype.resistance to quinolone antibiotics has been associated with single-nucleotide polymorphisms (snps) in the quinolone resistance-determining region (qrdr) of gyra. mutations in the gyra gene were compared by using mutant populations derived from wild-type salmonella enterica serovar enteritidis and its isogenic muts::tn10 mutator counterpart. spontaneous mutants arising during nonselective growth were isolated by selection with either nalidixic acid, enrofloxacin, or ciprofloxacin. qrdr snps were ...200415215081
[toxicological effects of copper on human intestinal caco-2 cells].to study the toxicological effects of copper on human intestinal caco-2 cells.200415211793
humoral immune response in hens naturally infected with salmonella enteritidis against outer membrane proteins and other surface structural antigens.a simple procedure for obtaining surface exposed antigens of salmonella enteritidis is described. a heat treatment of whole bacteria in saline solution induced the release of small membrane vesicles containing outer membrane components as well as surface appendage components, such as fimbriae and flagellin. the characterization of the structural components of this extract, called he, was established by sds-page and immunoblotting using polyclonal and monoclonal specific antibodies. five major gr ...200415210078
salmonella-based rodenticides and public health.several countries still permit strains of salmonella enterica serotype enteritidis, a leading cause of gastrointestinal illness in humans, to be used in rat baits. to assess the human health risk associated with such rat bait, we first reviewed historic data on health hazards associated with ratin, a rodenticide that was used in europe until the early 1960s. ratin caused outbreaks of human illness, including several deaths. we then compared s. enteritidis isolated from a current commercial produ ...200415207046
ovarian cell-mediated immune response to salmonella enteritidis infection in laying hens (gallus domesticus).the aim of this study was to examine the response of cell-mediated ovarian immunity against salmonella infection in hens. laying hens were injected intraperitoneally with pbs (control) or salmonella enteritidis (se). ovarian stroma containing stromal follicles, small white follicles (swf), and third largest (f3) and the largest (f1) follicles were collected 12 or 24 h after inoculation and fixed in periodate-lysine-paraformaldehyde. frozen sections were stained first for cd3+, cd4+, or cd8+ t ce ...200415206628
cocaine hepatotoxicity and its potentiation by lipopolysaccharide: treatment and gender effects.this study was conducted to investigate the effect of a 7-day treatment as well as the influence of gender on cocaine hepatotoxicity (ch). lipopolysaccharide (lps) potentiation of ch was also investigated. male and female cf-1 mice were orally administered 20 mg/kg body weight cocaine hydrochloride once daily for 7 days. four hours after the last cocaine administration, the mice were administered 12 x 10(6) eu lps (or equal volume of sterile saline) intraperitoneally. plasma alanine aminotransfe ...200415204719
endovascular stent-graft for thoracic aorta aneurysm caused by salmonella.we describe the placement of an endovascular stent-graft in a patient with mycotic aneurysm of the descending thoracic aorta caused by salmonella. endovascular grafting combined with antibiotic therapy in thoracic mycotic aneurysms might represent an alternative to conventional surgery in patients with high operative risk.200415201011
endovascular repair for multiple salmonella mycotic aneurysms of the thoracic aorta presenting with cardiovocal syndrome.salmonella mycotic thoracic aortic aneurysm is a rare but life-threatening condition. we report a 59-year-old man with two salmonella mycotic thoracic aortic aneurysms, presented with fever and chills associated with hoarseness due to left vocal cord palsy (cardiovocal syndrome). successful endovascular repair was performed using two talent thoracic stent-graft devices deployed separately to cover the two mycotic aneurysms. subsequent computed tomography at 12 months after the operation confirme ...200415201010
the recognition and modification sites for the bacterial type i restriction systems kpnai, styseai, styseni and stysgi.using an in vivo plasmid transformation method, we have determined the dna sequences recognized by the kpnai, styseai, styseni and stysgi r-m systems from klebsiella oxytoca strain m5a1, salmonella eastbourne, salmonella enteritidis and salmonella gelsenkirchen, respectively. these type i restriction-modification systems were originally identified using traditional phage assay, and described here is the plasmid transformation test and computer program used to determine their dna recognition sequ ...200415199175
interactions between high pressure homogenization and antimicrobial activity of lysozyme and lactoperoxidase.it was the objective of this work to evaluate the effect of high pressure homogenization on the activity of antimicrobial enzymes such as lysozyme and lactoperoxidase against a selected group of gram positive and gram negative species inoculated in skim milk. lactobacillus helveticus, lactobacillus plantarum and listeria monocytogenes were the most pressure resistant species while bacillus subtilis, pseudomonas putida, salmonella typhimurium, staphylococcus aureus, proteus vulgaris and salmonell ...200415193800
outbreak of salmonella serotype enteritidis infections associated with raw almonds--united states and canada, 2003-2004.on may 12, 2004, the oregon state public health laboratory identified a cluster of five patients infected with salmonella enterica serotype enteritidis (se) isolates that were matched by using two-enzyme pulsed-field gel electrophoresis (pfge). the five patients were from four oregon counties; their onsets of illness occurred during february-april 2004. a subsequent investigation, still ongoing, has identified a total of 29 patients in 12 states and canada with matching se isolates, since at lea ...200415190247
the effect of temperature on food poisoning: a time-series analysis of salmonellosis in ten european countries.we investigated the relationship between environmental temperature and reported salmonella infections in 10 european populations. poisson regression adapted for time-series data was used to estimate the percentage change in the number of cases associated with a 1 degree c increase in average temperature above an identified threshold value. we found, on average, a linear association between temperature and the number of reported cases of salmonellosis above a threshold of 6 degrees c. the relatio ...200415188714
[occurrence of phage types of non-typhoid salmonella serovar in the slovak republic 2000-2003].salmonellae of non-typhoidal serovars are the most important pathogens involved in foodborne diseases in humans all over the world. the incidence rates of two major salmonella serovars, i.e. s. enterica serovar enteritidis (s. enteritidis) and s. enterica serovar typhimurium (s. typhimurium), in the slovak republic in 2000-2003 are given. over the period studied, 829 s. enteritidis strains and 258 s. typhimurium strains isolated from patients with salmonellosis were investigated in the national ...200415185632
a serological survey for pathogens in old fancy chicken breeds in central and eastern part of the netherlands.to get an impression of the presence of pathogens in multi-aged flocks of old fancy chicken breeds in the netherlands, plasma samples originating from 24 flocks were examined for antibodies against 17 chicken pathogens. these flocks were housed mainly in the centre and east of the netherlands, regions with a high poultry density. the owners of the tested flocks showed their chicken at national and international poultry exhibitions. antibodies against avian influenza, egg drop syndrome '76 virus, ...200415185615
uptake and replication of salmonella enterica in acanthamoeba rhysodes.the ability of salmonellae to become internalized and to survive and replicate in amoebae was evaluated by using three separate serovars of salmonella enterica and five different isolates of axenic acanthamoeba spp. in gentamicin protection assays, salmonella enterica serovar dublin was internalized more efficiently than salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium in all of the amoeba isolates tested. the bacteria appeared to be most efficiently internalize ...200415184177
medium-chain fatty acids decrease colonization and invasion through hila suppression shortly after infection of chickens with salmonella enterica serovar enteritidis.the most common source of salmonella infections in humans is food of poultry origin. salmonella enterica serovar enteritidis has a particular affinity for the contamination of the egg supply. in this study, the medium-chain fatty acids (mcfa), caproic, caprylic, and capric acid, were evaluated for the control of salmonella serovar enteritidis in chickens. all mcfa were growth inhibiting at low concentrations in vitro, with caproic acid being the most potent. contact of salmonella serovar enterit ...200415184160
effects of furazolidone pretreatment of salmonella enteritidis pt4 at sub- and suprainhibitory concentrations on phagocytosis and intracellular survival in chicken macrophages.the antimicrobial effect of the nitrofuran derivative furazolidone at sub- and suprainhibitory concentrations on salmonella enteritidis pt4 and the influence with regard to interaction with avian macrophages was investigated in this study. phagocytosis of furazolidone-sensitive (fzs) s. enteritidis with chicken macrophages in the presence of furazolidone at concentrations of 1/8, 1/4, 1/2 and 8x mic resulted in an increase in the rate of phagocytic killing of approximately 3-, 6-, 6.5- and 9-fol ...200415182998
screening of selected indigenous plants of lebanon for antimicrobial activity.the objective of this study is to test in vitro the antimicrobial efficacy of 39 water and 39 methanol extracts derived from different parts of 27 indigenous wild plant species that have been commonly used in lebanese folk medicine. the antimicrobial efficacy was determined using the single disk diffusion method, with 10 and 20 microl load extract volume per disc. nine test microorganisms were used namely, escherichia coli, proteus sp., pseudomonas aeruginosa, shigella dysenteria, salmonella ent ...200415182897
cell-mediated immune responses to a killed salmonella enteritidis vaccine: lymphocyte proliferation, t-cell changes and interleukin-6 (il-6), il-1, il-2, and ifn-gamma production.two experimental approaches were used to investigate the immunological responses of chickens to a commercial killed salmonella enteritidis (se) vaccine. in the first, the effects of host age on antigen-specific proliferative responses and cytokine production were examined. compared with non-vaccinated controls, 4-wk-old vaccinated chickens showed higher proliferation to se lps and flagella. the lymphoproliferation responses to these antigens of 8-mo-old vaccinated chickens were not different com ...200415178000
method for assessment of functional affinity of antibodies for live bacteria.a rapid and convenient method for assessment of functional affinity of antibodies against live bacteria is described. when a combination of immunomagnetic separation (ims) with bioluminescent or fluorescent genetic labelling of the cells was employed, the method showed good correlation with plate count. however, the use of reporter bacteria allowed results to be obtained within 1 h compared with days using conventional methods. due to its lower detection limit, the bioluminescent assay performed ...200415177903
mesenteric cyst infected with non-typhoidal salmonella infection.although extra-intestinal non-typhoidal salmonella infections are common in developing countries, infection of the mesenteric cyst with salmonella enteritidis is an extremely rare occurrence. review of the english literature has revealed one report up to this date. the case of a 4-year-old boy with a mesenteric cyst infected with salmonella enteritidis is presented.200415168049
long-term alterations in neuroimmune responses after neonatal exposure to lipopolysaccharide.fever is an integral part of the host's defense to infection that is orchestrated by the brain. a reduced febrile response is associated with reduced survival. consequently, we have asked if early life immune exposure will alter febrile and neurochemical responses to immune stress in adulthood. fourteen-day-old neonatal male rats were given escherichia coli lipopolysaccharide (lps) that caused either fever or hypothermia depending on ambient temperature. control rats were given pyrogen-free sali ...200415163684
geographically dependent distribution of gyra gene mutations at codons 83 and 87 in salmonella hadar, and a novel codon 81 gly to his mutation in salmonella enteritidis.in all, 90 nalidixic acid-resistant clinical strains of salmonella hadar and salmonella enteritidis isolated in norway but of predominantly foreign origin were subjected to sequencing of the gyra, gyrb, parc and pare genes. all the isolates contained at least one mutation in gyra codon 83 or codon 87. a highly significant correlation between mutations in gyra codon 83 and strains originating from southeast asia was found in s. hadar but not in s. enteritidis. a novel gyra codon 81 gly to his mut ...200415153158
rapid, specific detection of salmonella enteritidis in pooled eggs by real-time pcr.an assay was developed for the specific detection of salmonella enteritidis in eggs with the use of an application of the fluorogenic 5' nuclease assay (taqman). in this assay, a segment of the gene sefa specific to salmonella group d strains such as salmonella enteritidis was used. the amplification of the target gene products was monitored in real-time by incorporating a fluorescent dye-labeled gene-specific probe in the pcr reaction. this method correctly detected and distinguished salmonella ...200415151219
frequency and polymorphism of sope in isolates of salmonella enterica belonging to the ten most prevalent serotypes in england and wales.translocated effector protein, sope, leads to actin cytoskeletal rearrangements and membrane ruffling. only a subset of salmonella enterica serotypes possess sope, with the majority of sope-carrying s. enterica serotype typhimurium associated with epidemics. using real-time pcr and sequencing, sope was investigated in the ten most prevalent serotypes of s. enterica in england and wales in 2001. sope was identified in s. typhimurium definitive phage types 29, 44, 49, 204b and 204c, all of which e ...200415150335
multiple genetic typing of salmonella enteritidis phage-types 4, 6, 7, 8 and 13a isolates from animals and humans in the uk.salmonella enterica serovar enteritidis is a common cause of salmonellosis in people in the uk. this study aimed to assess the degree of genetic diversity among animal and human isolates from uk, wales and northern ireland. a total of 250 isolates from humans (n = 59) and animals or their environment (n = 191), belonging to the most common phage-types, were fingerprinted by a combination of pfge, ps ribotyping and plasmid profiling. the different techniques identified different degrees of polymo ...200415145497
yersinia enterocolitica leads to transient induction of tnf-alpha and activates nf-kappab in synovial fibroblasts.the importance of the presence of bacterial antigen or even living bacteria for the pathogenesis of reactive arthritis has been discussed increasingly ever since bacterial antigen was found in inflamed joints. bacteria may persist in the body and drive the local immune response, maintaining arthritis. cytokines, in particular tumor necrosis factor-alpha (tnf-alpha) are essential for bacterial elimination. in reactive arthritis, the course of the disease is influenced by several cytokines, includ ...200415144120
comparison of methods for differentiation of salmonella enterica serovar enteritidis phage type 4 isolates.to compare molecular typing methods for the differentiation of salmonella enterica serovar enteritidis phage type (pt) 4 isolates that allowed for the determination of their genetic relatedness.200415141870
effect of food protein supplements on salmonella enteritidis infection and prevention in laying hens.reduction of intestinal colonization of salmonella enteritidis (se) during the grow-out period is crucial to provide safer eggs, minimize economic losses, and reduce the spread of human salmonellosis. in the search for novel elimination and prevention methods based on feed supplementation, the effects of feed supplemented with nonimmunized egg yolk powder (did not contain anti-s. enteritidis antibodies), immunized egg yolk powder (with anti-s. enteritidis antibodies), egg yolk proteins, egg whit ...200415141832
effects of a lactoperoxidase-thiocyanate-hydrogen peroxide system on salmonella enteritidis in animal or vegetable foods.lactoperoxidase (lpo) from skim milk was purified by ion-exchange chromatography. the purified protein was used to catalyze the oxidation of thiocyanate by h2o2 in an antibacterial system (lpo system). the lpo system was used to inactivate or inhibit salmonella enteritidis in tomato juice, carrot juice, milk, liquid whole egg, and chicken skin extract under various conditions. the system was found to be more effective against the organism in vegetable juices than in animal products, at low ph th ...200415135956
subpopulation characteristics of egg-contaminating salmonella enterica serovar enteritidis as defined by the lipopolysaccharide o chain.characterization of salmonella enterica serovar enteritidis was refined by incorporating new data from isolates obtained from avian sources, from the spleens of naturally infected mice, and from the united kingdom into an existing lipopolysaccharide (lps) o-chain compositional database. from least to greatest, the probability of avian isolates producing high-molecular-mass lps o chain ranked as follows: pooled kidney, liver, and spleen; intestine; cecum; ovary and oviduct; albumen; yolk; and who ...200415128529
acute renal tubular dysfunction in association with salmonella enteritidis.a thirteen-year-old boy presented with acute renal tubular dysfunction after an infection with salmonella enteritidis. the child recovered following treatment with ciprofloxacin for a week.200415123871
differences in the carriage and the ability to utilize the serotype associated virulence plasmid in strains of salmonella enterica serotype typhimurium investigated by use of a self-transferable virulence plasmid, pog669.most strains of salmonella enterica subspecies enterica serotype typhimurium (s. typhimurium) naturally harbour a virulence plasmid which carries the salmonella plasmid virulence (spv) genes. however, isolates belonging to certain phage types are generally found without the plasmid. we have utilized a self-transferable virulence plasmid, pog669 to investigate the effect of introduction of spv genes into strains of such phage types. the use of the co-integrate plasmid, pog669, was validated on a ...200415120160
analysis of chicken tlr4, cd28, mif, md-2, and litaf genes in a salmonella enteritidis resource population.salmonella enteritidis is a foodborne pathogen that negatively affects both animal and human health. genetic variations in response to pathogenic se colonization or to se vaccination were measured in a chicken resource population. outbred broiler sires and 3 diverse, highly inbred dam lines produced 508 f1 progeny that were evaluated for either bacterial colonization after pathogenic se inoculation or circulating antibody level after se vaccination. five candidate genes were selected for study, ...200415109052
effect of orally administered egg yolk antibodies on salmonella enteritidis contamination of hen's eggs. 200415107039
nontyphoid salmonellosis in taiwan children: clinical manifestations, outcome and antibiotic resistance.the purposes of this study were to investigate the epidemiologic, clinical, and microbiologic features of patients with nontyphoid salmonellosis and to elucidate the impact of resistance on the outcome of nontyphoid salmonellosis in taiwan. the authors also sought to develop a severity score to derive an objective guideline for antibiotic use in nontyphoid salmonellosis in the era of increasing antibiotic resistance.200415097441
chicken consumption is a newly identified risk factor for sporadic salmonella enterica serotype enteritidis infections in the united states: a case-control study in foodnet sites.the sources of sporadic salmonella enterica serotype enteritidis (se) infections in the united states are unclear. to determine risk factors for sporadic se infection, we conducted a population-based case-control study in 5 foodborne disease active surveillance network surveillance areas. during the 12-month study, 396 cases of se infection were ascertained. among the 182 case patients and 345 controls, se infection was univariately associated with international travel (matched odds ratio [mor], ...200415095196
dramatic decrease in the incidence of salmonella serotype enteritidis infections in 5 foodnet sites: 1996-1999.salmonella serotype enteritidis (se) emerged as the most common salmonella serotype among infected persons in the united states during the 1980s and 1990s, with infections reaching a peak in 1995. during the past decade, farm-to-table control measures have been instituted in the united states, particularly in regions with the highest incidence of se infection. we report trends in the incidence of se in the 5 original surveillance areas of the foodborne diseases active surveillance network during ...200415095182
efficacy of egg cleaning compounds on eggshells contaminated with salmonella enterica serovar enteritidis.salmonella enteritidis infections of egg contents can be related to external contamination of the shell. in this study, the efficacy of three commercial cleaning and/or sanitizing compounds (sodium carbonate, sodium hypochlorite, and potassium hydroxide) was evaluated for bactericidal activity at ph values of 10, 11, and 12 against various concentrations (10(2), 10(4), or 10(6) cfu/ml) of salmonella enteritidis inoculated onto the eggshell surface. efficacy of these chemical agents was also asse ...200415083722
reduction of escherichia coli o157:h7 and salmonella enterica serovar enteritidis in chicken manure by larvae of the black soldier fly.green fluorescent protein-labeled escherichia coli o157:h7 and salmonella enterica serovar enteritidis were inoculated at 10(7) cfu/g into cow, hog, or chicken manure. ten- or 11-day-old soldier fly larvae (hermetia illucens l.) (7 to 10 g) were added to the manure and held at 23, 27, or 32 degrees c for 3 to 6 days. soldier fly larvae accelerated inactivation of e. coli o157:h7 in chicken manure but had no effect in cow manure and enhanced survival in hog manure. the initial ph values of the ho ...200415083719
salmonella enteritidis infections, united states, 1985-1999.salmonella enterica serotype enteritidis emerged as an important illness during the 1980s. investigations showed that consumption of undercooked eggs was the major risk factor for disease, and a variety of prevention and control efforts were initiated during the 1990s. we describe sporadic infections and outbreaks of s. enteritidis in the united states from 1985 through 1999 and discuss prevention and control efforts. after reaching a high of 3.9 per 100,000 population in 1995, s. enteritidis in ...200415078589
in vitro activity of nitrofuran derivatives on growth and morphology of salmonella enterica serotype enteritidis.to evaluate the effect of nitrofuran derivatives furazolidone (fz) and nitrofurantoin (nf) on salmonella enterica serovar enteritidis pt4 in vitro, with regard to cell growth, morphology and ultrastructure.200415078517
the effect of killed salmonella enteritidis vaccine prior to induced molting on the shedding of s. enteritidis in laying hens.effects of administering killed salmonella enterica serovar enteritidis (se) vaccines to laying hens prior to induced molting on egg production and on shedding of se were investigated. forty hens were vaccinated with one of two se vaccines available commercially in the united states and japan. twenty-five days after vaccination, feed was withdrawn for 2 wk from 20 vaccinated plus 10 unvaccinated hens to induce molt. four days after molt induction, all hens were challenged with a dose of 2.4 x 10 ...200415077813
effects of prior coinfection with different salmonella serovars on the progression of a salmonella enterica serovar enteritidis infection in hens undergoing induced molt.four trials were conducted to evaluate whether prior infection with salmonella enterica serovar typhimurium (s. typhimurium) or salmonella enterica serovar muenchen (s. muenchen) would modify the severity or the transmission of salmonella enterica serovar enteritidis (s. enteritidis) challenge in hens undergoing molt via feed withdrawal. hens were separated into two groups where one group received a prior s. typhimurium or s. muenchen infection, whereas the other group remained untreated until s ...200415077810
detection of airborne salmonella enteritidis in the environment of experimentally infected laying hens by an electrostatic sampling device.bacteriologic culturing of environmental samples taken from sources such as manure pits and egg belts has been the principal screening tool in programs for identifying commercial laying flocks that have been exposed to salmonella enteritidis and are thus at risk to produce contaminated eggs. because airborne dust and aerosols can carry bacteria, air sampling offers a potentially efficient and inexpensive alternative for detecting s. enteritidis in poultry house environments. in the present study ...200415077808
flagella and curli fimbriae are important for the growth of salmonella enterica serovars in hen eggs.salmonella enterica serovar enteritidis is unable to multiply in the albumen of fresh eggs and must gain access to the yolk contents in order to multiply to a high level (>10(6) c.f.u. per ml egg contents). as human salmonella infections resulting from the consumption of infected eggs more frequently involve serovar enteritidis phage type (pt) 4 than other serovars or pts, a number of isolates of various s. enterica serovars were examined for their ability to multiply to a high level in eggs ove ...200415073315
development of a multiplex pcr technique for detection and epidemiological typing of salmonella in human clinical samples.we have developed a multiplex pcr assay for salmonella detection and epidemiological typing. six sets of primers were designed to detect the major salmonella serotypes and phage types in spain. an internal amplification control was designed in order to detect pcr inhibition. the different amplification profiles obtained allowed us to detect salmonella bacteria and to distinguish the clinically prevalent salmonella enterica serotypes enteritidis, typhimurium and subspecies i serotype 4,5,12:i:-. ...200415071035
immunogenic properties of the salmonella atypical fimbriae in balb/c mice.components of the salmonella atypical fimbriae (saf) were investigated for potential inclusion in a salmonella vaccine. recombinant histidine-tagged safb chaperone complexed with safd adhesin was expressed in escherichia coli and purified. starch microparticles were used, as an adjuvant and recombinant cholera toxin b subunit (rctb) was included as a mucosal antigen-uptake enhancer. balb/c mice were immunized orally or subcutaneously with safb/d- and rctb-conjugated microparticles and nasally or ...200415063568
antibiotic resistance in salmonella enterica serotypes typhimurium, enteritidis and infantis from human infections, foodstuffs and farm animals in italy.the antimicrobial susceptibility of isolates of salmonella enterica serotypes typhimurium, enteritidis, and infantis isolated from humans, foodstuffs and farm animals in italy between 1999 and 2001 was examined. all the isolates were susceptible to cefotaxime and ciprofloxacin, but high rates of resistance were observed for several other drugs, especially for s. typhimurium. the rates of resistance and multiresistance were generally higher among animal and food isolates than in human strains; co ...200415061499
[mechanisms of resistance to beta lactams and nalidixic acid in clinical isolates of salmonella enteritidis serotype hadar and bsilla]. 200415056446
salmonella enterica subsp. enterica serovar enteritidis harbours cole1, cole2, and rolling-circle-like replicating plasmids.using dna hybridization, at least three distinct groups of low molecular mass plasmids were identified in salmonella enterica subsp. enterica serovar enteritidis. after sequencing representative plasmids from each group, we concluded that they belonged to cole1, cole2, and rolling-circle-like replicating plasmids. plasmid pk (4245 bp) is a representative of widely distributed cole1 plasmids. plasmid pp (4301 bp) is homologous to cole2 plasmids and was present predominantly in single-stranded dna ...200415052312
tubular glands of the isthmus are the predominant colonization site of salmonella enteritidis in the upper oviduct of laying hens.salmonella enterica serovar enteritidis is the serovar most frequently isolated from chicken eggs. colonization of the upper oviduct of hens is believed to play an important role in egg contamination. the interaction of s. enteritidis with gland epithelial cells of the isthmus and the magnum was, therefore, studied in vitro and in vivo. in the first experiment, s. enteritidis bacteria were added to confluent monolayers of primary cultures of chicken tubular epithelial cells of the isthmus (ictec ...200415049486
testing two lactobacillus plantarum and lactobacillus acidophilus strains for their suitability as a lipoid probiotic.two strains of lactobacilli (lactobacillus acidophilus t-135 and lactobacillus plantarum 4/97) were selected in order to study their inhibitory properties against frequent udder pathogens (escherichia coli, staphylococcus aureus, streptococcus agalactiae, streptococcus uberis, salmonella enteritidis and bacillus pumilus), their production of organic acids as well as their ability to survive on the teat skin, the teat duct mucosa and in a lipoid emulsion. both strains inhibited the tested pathoge ...200415046462
antibacterial effects of carica papaya fruit on common wound organisms.the purpose of the study was to investigate antibacterial activity of ripe and unripe carica papaya on selected micro-organisms. cultures of micro-organisms were routinely maintained in nutrient agar slants at 4 degrees c. extracts of immature, mature and ripe carica papaya fruit were obtained by separately grinding factions of the epicarp, endocarp and seeds and filtering them through gauze. sensitivity tests were conducted by adding 0.06 ml of extract to agar wells (6 mm diameter) prepared fro ...200315040064
rapid expression of chemokines and proinflammatory cytokines in newly hatched chickens infected with salmonella enterica serovar typhimurium.poultry meat and eggs contaminated with salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium are common sources of acute gastroenteritis in humans. however, the exact nature of the immune mechanisms protective against salmonella infection in chickens has not been characterized at the molecular level. in the present study, bacterial colonization, development of pathological lesions, and proinflammatory cytokine and chemokine gene expression were investigated in the l ...200415039338
injury recovery of foodborne pathogens in high hydrostatic pressure treated milk during storage.bacteria are expected to be injured or killed by high hydrostatic pressure (hhp). this depends on pressure levels, species and strain of the microorganism and subsequent storage. injured bacteria may be repaired which could affect the microbiological quality of foodstuffs with an important safety consideration especially in low acid food products. in this study two gram-positive (listeria monocytogenes ca and staphylococcus aureus 485) and two gram-negative (escherichia coli o157:h7 933 and salm ...200415039101
detection of specific antibodies against deflagellated salmonella enteritidis and s. enteritidis flic-specific 9kda polypeptide.specific antibody levels of laying hens and young chickens experimentally infected with salmonella enteritidis and vaccinated farm flocks were evaluated by enzyme-linked immunosorbent assays (elisas) with two different antigens, deflagellated s. enteritidis whole cell (dewc) and s. enteritidis flic-specific 9kda polypeptide (sep9). infected laying hens excreted s. enteritidis throughout the experimental period, and the specific antibody titers in dewc-elisa, were significantly higher than the un ...200415019102
dietary fructo-oligosaccharides and inulin decrease resistance of rats to salmonella: protective role of calcium.we have shown recently that rapid fermentable fructo-oligosaccharides (fos) decreased resistance of rats towards salmonella. it is not known whether inulin (which is fermented more gradually) has similar effects or whether buffering nutrients can counteract the adverse effects of rapid fermentation.200415016747
[miller-fisher syndrome: clinical features, associated infections and clinical course in 8 cases].miller-fisher syndrome (mfs) is considered the most common variant of guillain-barré syndrome (gbs) and is characterized by the clinical triad of ophthalmoplegia, ataxia and areflexia. respiratory involvement and relapses are unusual. patients with mfs usually have a good recovery and no residual deficits. we describe the clinical features, associated infections and evolution in eight patients with mfs.200415012892
random amplified polymorphic dna and phenotyping analysis of salmonella enterica serovar enteritidis isolates collected from humans and poultry in uruguay from 1995 to 2002.molecular and phenotyping techniques were applied to study salmonella enterica serovar enteritidis strains both from human cases of infection and of avian origin isolated in uruguay from 1995 to 2002. a group of 62 isolates was subjected to random amplified polymorphic dna (rapd) assay and analysis of antibiotic resistance patterns. twenty-one of these strains were further characterized by phage typing and analysis of their protein expression profiles. rapd fingerprinting with five different pri ...200415004068
prosthetic valve endocarditis caused by salmonella enteritidis.until now, only 12 cases of prosthetic valve endocarditis caused by salmonella spp. have been reported in the english literature. high complication and mortality rates, a fulminant course and the requirement for early surgical intervention deserve special attention in this kind of infective endocarditis. a new case of salmonella prosthetic valve endocarditis complicated by sepsis-induced cholestasis and a literature review are presented in this report.200415000568
transmission of salmonella between broiler chickens fed with fermented liquid feed.in the light of food safety and the control of salmonella at chicken farms, fermented liquid feed (flf) was studied. this moistened feed reduced the susceptibility of chickens for salmonella. to assess the effect of the fermented feed on the transmission of salmonella between chickens, a transmission experiment was performed. salmonella shedding was followed within groups of two susceptible chickens together with two previously inoculated chickens. the between-chicken transmission was quantified ...200414979596
role of nicotinamide adenine dinucleotide phosphate oxidase 1 in oxidative burst response to toll-like receptor 5 signaling in large intestinal epithelial cells.the nadph oxidase 1 (nox1) is a gp91(phox) homologue preferentially expressed in the colon. we have established primary cultures of guinea pig large intestinal epithelial cells giving 90% purity of surface mucous cells. these cells spontaneously released superoxide anion (o(2)(-)) of 160 nmol/mg protein/h and expressed the nox1, p22(phox), p67(phox), and rac1 mrnas, but not the gp91(phox), nox4, p47(phox), p40(phox), and rac2 mrnas. they also expressed novel homologues of p47(phox) and p67(phox) ...200414978110
effect of disinfectants on the metabolism of salmonella enterica serovar enteritidis.antimicrobial activity of 19 commercially manufactured disinfectant substances on a salmonella enteritidis strain was determined. the substances represented 8 quaternary ammonium salts (qas) and 10 qas combined with other additives. the antimicrobial efficacy was characterized by influencing the growth of bacterial cells expressed as mic and ed50 values as well as by the inhibition of the incorporation rate of 14c-adenine and 14c-leucine. according to their efficacy the disinfectants were divide ...200314976722
salmonella osteomyelitis of the thoracic spine: an unusual presentation.a case of thoracic vertebral osteomyelitis due to salmonella enteritidis phage type 2 in an immunocompetent patient is reported. the patient initially presented with abdominal, urinary and chest symptoms, which were followed by a large pleural effusion. the infection was successfully treated with ciprofloxacin. this is the only case of salmonella thoracic vertebral osteomyelitis in an immunocompetent patient reported in the english literature.200414970302
antimicrobial susceptibilities and epidemiological analysis of salmonella enteritidis isolates in korea by phage typing and pulsed-field gel electrophoresis.a total of 81 isolates of salmonella enteritidis were analyzed by antibiotic susceptibility, phage typing, and pulsed-field gel electrophoresis (pfge). thirty-two isolates came from broiler carcasses and pig feces, and 49 isolates were from humans in seoul and suburbs of seoul, korea. antibiotic resistance was most prevalent among human isolates. of human isolates, 89.8% were resistant to more than two antibiotics, while 64.7% of poultry isolates and 13.3% of pig isolates showed multiple resista ...200414968957
changes in salmonella enteritidis contamination in two layer lines vaccinated during the rearing period. 200314965141
genetic variability in the anti-microbial activity of hen egg white. 200314965115
in vivo characterization of lactobacillus johnsonii fi9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry.to test the efficacy of lactobacillus johnsonii fi9785 in reducing the colonization and shedding of salmonella enterica serotype enteritidis, escherichia coli o78:k80 and clostridium perfringens in poultry.200414962040
[course of the epidemic acute gastroenteritis due to salmonella enteritidis gaertner in the state boys school in brno in september, 1950]. 195214939974
pyogenic liver abscess: a review with a case report of a solitary abscess caused by salmonella enteritidis. 195214937218
the isolation of salmonella dublin from an outbreak of dysentery in pigs. 195114867190
salmonella enteritidis in a whale. 195014860957
[behaviour of toxins of clostridium botulinum and salmonella enteritidis towards antibiotics, chemotherapeutics and dyes]. 195114837307
[elimination of a salmonella enteritidis infection in test animals by means of an aerosol and conclusions to be drawn with regard to future therapy]. 195114815633
[multiplication of bacteriophage and bacteria in mice infected with salmonella enteritidis, var. danysz]. 195014811115
[chloromycetine in salmonellosis therapy]. 195114804658
[liberation of glucido-lipidic antigen of salmonella enteritidis var. danysz by bacteriophage lysis]. 195014799974
resistance to infection with salmonella enteritidis and mycobacterium tuberculosis of mice fed different diets. 195014778962
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