Publications

[mobilization using incompatibility group p1 plasmids in strains of pseudomonas pseudomallei and pseudomonas mallei as potential vectors for dna cloning].	the cells of pseudomonas pseudomallei and pseudomonas mallei have been shown to serve as recipients for the plasmid rsf1010 and its recombinant derivatives pva1 and pva4. the conjugative plasmids rp1 and pth10 of the incompatibility group p1 are able to mobilize the nontransmissive vector plasmids for conjugation transfer into pseudomonas pseudomallei and pseudomonas mallei strains. the smr determinant of the plasmid rsf1010 is expressed in the latter strains. these data makes the mentioned vect ...	1992	1280770
melioidosis: when in doubt leave the quinolone alone!		1992	1280318
structure of the polysaccharide chains of pseudomonas pseudomallei lipopolysaccharides.	the pathogenic bacterium pseudomonas pseudomallei strain 57576 produces two partially o-acetylated o-antigenic polysaccharides (ps-i and ps-ii). methylation analysis and 1h and 13c nmr spectroscopy, including noe experiments, showed ps-i to have the structure [formula: see text] and ps-ii to have the structure [formula: see text] where 6dmanhep is the unusual higher sugar 6-deoxy-d-manno-heptose. ps-ii is produced also by p. pseudomallei strains 100 and 110, and ps-i and o-deacetylated ps-ii by ...	1992	1280183
an outbreak of melioidosis in imported primates in britain.	an outbreak of melioidosis, a bacterial infection caused by pseudomonas pseudomallei, was identified in a batch of feral cynomolgus monkeys (macaca fascicularis) imported to britain from the philippines. thirteen confirmed or possible cases occurred among a batch of 50 animals. subsequent investigations revealed that the infection was uncommon among imported primates from a variety of sources, although three other cases were identified in monkeys imported from indonesia. the majority of the affe ...	1992	1279882
a case of human melioidosis originating in south-west western australia.	to report the first human case of autochthonous melioidosis in temperate australia (latitude 31 degrees 10's) and to describe the extent of the presence of the causative agent, pseudomonas pseudomallei, in southwest western australia.	1992	1279367
[demonstration of pseudomonas pseudomallei (whitmore's bacillus) in the mud of iranian ricefields (author's transl)].	whitmore bacillus had killed a horse and a mule in a herd bred for serum production in 1970 (baharsefat and amjadi). out of 157 soil samples from rice fields in northern iran it was possible to isolate 19 times pseudomonas pseudomallei (types i and ii) possessing a very high pathogenicity for animals. attempts at an evaluation of the human incidence of the disease.	1975	1243750
induction of microbial variants of pseudomonas pseudomallei in cultured rabbit alveolar macrophages.	cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of pseudomonas pseudomallei. variants were iduced only by normal alveolar macrophages. the variants reverted to typical pseudomonas forms either spontaneously or upon transfer into broth or agar.	1975	1220870
bovine melioidosis in south-eastern queensland.	acute fatal infection with pseudomonas pseudomallei caused pneumonia, placentitis and endometritis in a pregnant cow. pneumonia was also present in the foetal lung. mononuclear cell response with extensive karyorrhexis occurred in maternal and foetal lung and the same cellular pathology, but with diffuse distribution, was responsible for plaques on the endometrium. a more chronic disease with encapsulated caseous lesions in the lung, together with arthritis, occurred in a bull on the same proper ...	1975	1191141
human-to-human transmission of pseudomonas pseudomallei.	melioidosis, the clinical manifestation of infection with pseudomonas pseudomallei, has occurred infrequently in american citizens; almost all reported cases have been in vietnam veterans, usually associated with respiratory disease. a vietnam veteran from mississippi developed chronic prostatitis, with no other clinical manifestations, during service in vietnam, and p. pseudomallei was isolated from prostatic secretions 2 years after his return to the united states. the patient had had sexual c ...	1975	1174405
pulmonary melioidosis. observations in thirty-nine cases.	during the 6-year period from 1965 through 1970, 39 patients with pulmonary melioidosis were treated at fitzsimons army medical center. although this is a disease with a well-defined endemic area that does not include the united states, cases will no doubt continue to be seen in this country. these cases may result from acute infection in the endemic area, with subsequent travel to this country, or from delayed reactivation of a latent infection acquired months or years earlier. given the potent ...	1975	1163890
differentiation between pseudomonas cepacia and pseudomonas pseudomallei in clinical bacteriology.	if only conventional laboratory tests are used, the widely occurring species pseudomonas cepacia may, due to its variability, sometimes be difficult to differentiate from the dangerous pathogen pseudomonas pseudomallei. in a comparative study using fresh isolates it is shown that animal inoculation seems to be a useful additional method in the differentiation. also the determination of the ability to utilize selected carbon sources such as starch, hydroxybenzoate and uracil is helpful in reachin ...	1975	1155112
surveillance of some infectious diseases among aircrew personnel in southeast asia.	a 2-year analysis of specimens among aircrew personnel in southeast asia is reported. stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (sgot) and antibodies to entamoeba histolytica, toxoplasma gondii, chikungunya and japanese encephalitis viruses, rickettsia tsutsugamushi, leptospira sp. and pseudomonas pseudomallei. one to four specimens each were obtained from 537 adult males and 20 females. there ...	1975	1100040
[the in vitro sensitivity of 24 strains of pseudomonas pseudomallei to antibiotics (author's transl)].		1976	1021818
[pseudomonas pseudomallei infections: clinical and bacteriologic significance (author's transl)].		1976	1021817
a retrospective serological suvey of royal marines previously exposed to pseudomonas pseudomallei in south east asia.	following the suggestion that it was possible that cases of melioidosis amongst those who had been exposed abroad in the past, might be escaping notice, 487 royal marines were examined by indirect haemagglutination studies. four hundred and eleven of these subjects had served for variable times in areas where melioidosis has been known to occur in indonesia and malaya, between 1960 and 1974, occupied in activities in the jungle and paddy fields during which exposure to the disease was to be expe ...	1976	1006764
terminal exacerbation of chronic melioidosis in new south wales.	a fatal case of melioidosis, thought to be the third recorded from new south wales, is presented. infection probably occurred in queensland. the patients presented with a subcutaneous abscess complicated by pyaemia, extensive lung involvement and septicaemic shock. the diagnosis was bacteriologically confirmed shortly before death, by isolation of pseudomonas pseudomallei from blood, pus swabs and tracheal aspirates. there is a need for greater awareness of this disease in persons who have resid ...	1976	994967
interaction between pseudomonas pseudomallei and cultured rabbit peritoneal macrophages.	the interaction between two strains of pseudomonas pseudomallei of different virulence with normal and immune rabbit peritoneal macrophages was compared in vitro. phagocytic activity of macrophages and bacterial survival within macrophages were dependent upon the virulence of the bacterial strain and the immune status of the macrophages. virulent bacteria were more resistant than the less virulent strain to phagocytosis and destruction. immune macrophages were more phagocytic and bactericidal th ...	1976	987839
adsorption of pseudomonas pseudomallei cytophilic antibodies onto rabbit alveolar macrophages.	cytophilic antibody has been demonstrated in sera from rabbits immunized with pseudomonas pseudomallei. this antibody passively adsorbed onto normal rabbit alveolar macrophages rendered the macrophages agglutinable by p. pseudomalei antigens and capable of displaying 'rosette' formation with polysaccharide-sensitized erythrocytes.	1976	974906
wound infection by an indigenous pseudomonas pseudomallei-like organism isolated from the soil: case report and epidemiologic study.	a 27-year-old farmer in the oklahoma panhandle was pinned under his overturned tractor for 2 hr and received superficial and deep lacerations. he contracted an infection of a pelvic wound with an organism that had cultural and biochemical characteristics identical to those of pseudomonas pseudomallei. identical organisms were recovered from soil taken from the site of the accident. the organism isolated from the wound proved to be less virulent in guinea pigs than usual laboratory strains of p. ...	1977	833442
melioidosis presenting as prostatitis--a case report from sabah.	previous reports of melioidosis in sabah are reviewed and a detailed account of a case, presenting as prostatitis, in a 40-year-old british male is given. the history suggested that the organism, pseudomonas pseudomallei, was transmitted by a fly which entered the eye. diagnosis was delayed and treatment presented some difficulty, the organism being relatively insensitive to amplicillin and gentamicin. co-trimoxazole was the most effective, followed by minocycline. cure was eventually achieved a ...	1978	725999
melioidosis in a sulphur-crested cockatoo (cacatua galerita).	a case of melioidosis in a sulphur-crested cockatoo (cacatua galerita) kept as a pet in townsville is described. this case was characterised by liver necrosis with both a heterophil and mononuclear cell inflammatory response associated with giant cells. pseudomonas pseudomallei was isolated by blood plate culture and guinea pig inoculation. this is the first case of melioidosis reported in a native bird in australia.	1978	687299
[phagocytability of pseudomonas pseudomallei].	experiments were conducted on the cell culture of macrophages of animals significantly differing by the extent of resistance to melioidosis; the presence of correlation between the extent of the animal natural immunity and the intensity of dying of the microbes in the test system was demonstrated. the causative agent of melioidosis proved to be more resistant to phagocytosis with guinea pig macrophages than e. coli. ps. aeruginosa and a. mallei. it was impossible to establish any relationship be ...	1978	665019
the isolation of pseudomonas pseudomallei from soil in north queensland.		1977	588173
use of stable, sensitized cells in an indirect microhemagglutination test for melioidosis.	two evaluations were carried out in this study. the first was a comparison of the standard tube test with the automated microtitration test for the detection of antibodies to pseudomonas pseudomallei by the indirect hemagglutination method. data from this comparison indicated that the tests were equivalent. the second evaluation consisted of reproducibility studies on two lots of pyruvic aldehyde-stabilized sensitized erythrocytes in comparison with freshly prepared sensitized erythrocytes in th ...	1977	557498
nosocomial infection due to pseudomonas pseudomallei: two cases and an epidemiologic study.	pseudomonas pseudomallei was recovered from urine specimens of two patients who acquired the organism after they were admitted to a hospital in a region in which it is endemic. both patients were diabetic and both had urethral catheters passed while they were hospitalized. samples of soil taken from the hospital grounds yielded an isolate of p. pseudomallei that was identical biochemically and by antibiogram to the two isolates recovered from the urine specimens of the two patients. the two pati ...	1979	542764
isolation of pseudomonas pseudomallei from clay layers at defined depths.	twelve strains of pseudomonas pseudomallei were isolated from the soil and water of a sheep paddock over a two-year period. the organism was recovered from the clay layer of the soil profile as well as from water that seeps into this layer during the "wet" season. five isolates were obtained before the commencement of the "wet" season; environmental factors appear to play an important role in the survival of ps. pseudomallei during the "dry" season. lower isolation rates were recorded than those ...	1979	507042
an improved screening technique for isolation of pseudomonas pseudomallei from clinical specimens.	a selective medium consisting of trypticase soy agar with 4% glycerol, 5 mg/l crystal violet, 50 mg/l neutral red and 4 mg/l of gentamicin was devised for isolation of pseudomonas pseudomallei from clinical specimens. absorption of neutral red was found to be suitable for differentiating this organism from other bacteria, while gentamicin was effective in selecting ps. pseudomallei from organisms commonly found in clinical material. the medium was more suitable for screening clinical specimens t ...	1979	460953
[effect of d,l-ureidosuccinic dihydrazide on the biological properties of pseudomonas pseudomallei and salmonella typhimurium].		1979	398137
identification of pseudomonas pseudomallei in the clinical laboratory.	ninety-one strains of pseudomonas pseudomallei were tested in the api 20e system and in equivalent conventional tests. except for utilisation of citrate there was good correlation between api and conventional tests. seven-digit profiles were constructed from each strain after 48 hours' incubation, and numerical codes 2 006 727, 2 206 706, 2 206 707, and 2 206 727 accounted for 77% of strains tested. api 20 e, in combination with tests for heat stability of alkaline phosphatase, resistance to col ...	1979	381328
melioidosis antibodies in commonwealth soldiers.	titres of melioidosis haemagglutinating antibodies of 1/40 or more were found in 18 of 905 british, australian, and new zealand soldiers serving in west malaysia. previous mild unsuspected melioidosis seemed to be responsible for these positive titres, which were more common in men exposed to surface water at work and during recreation. this accords with the current view that soil and surface water is the normal habitat of pseudomonas pseudomallei, the causal organism. pyrexia of unknown origin ...	1976	54528
pseudomonas pseudomallei: ii. laboratory and experimental studies in animals.	the cultural, biochemical characteristics and antibiotic sensitivity of strains of pseudomonas pseudomallei isolated from four cases of melioidosis admitted to ramathibodi hospital are described. the organisms were gram-negative bacilli often with bipolar staining. the colonies were wrinkled when incubated for long periods. the characteristic non-specific uptake of dye from media into the colonies and their musty or earthy odour rendered them easily distinguishable from other organisms. all stra ...	1975	49930