Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| absence of persistence and transfer of genetic material by recombinant escherichia coli in conventional, antibiotic-treated mice. | strain bst-1 is a derivative of escherichia coli k-12 that carries a plasmid designated pura-4 and is the expression system used by the upjohn company in the production of recombinant bovine somatotropin (rbst). this plasmid also encodes an ampicillin resistance gene. the plasmidless carrier strain, bst-1c, contains a gene for tetracycline resistance which is provided by the chromosomal insertion of the transposon tn10. therefore, bst-1 is resistant to ampicillin and tetracycline, while bst-1c i ... | 1993 | 7763898 |
| escherichia coli k12 does not colonize the gastrointestinal tract of fischer-344 rats. | the colonizing potential of escherichia coli k12 containing a vector coding for somidobove (bovine somatotropin) was determined. treated male and female fischer-344 rats were given a single oral gavage inoculum of sucrose with/without tetracycline (15 micrograms/ml). untreated control animals received similar drinking water regimes. all animals survived until termination. there were no clinical signs of toxicity observed and no treatment-related effect upon body weight, food consumption, or effi ... | 1993 | 7763897 |
| potential for gene transfer from recombinant escherichia coli k-12 used in bovine somatotropin production to indigenous bacteria in river water. | this study examined the transfer of the plasmid pbgh1, an expression vector for bovine somatotropin (bst), from escherichia coli k-12 strain w3110g [pbgh1] to indigenous microorganisms present in flasks containing missouri river water. strain lbb269 is a nalidixic acid-resistant derivative of w3110g which was used as a plasmid-free control strain in these studies. water samples were inoculated with strains w3110g [pbgh1] and lbb269; after 21 days of incubation the number of viable colony-forming ... | 1993 | 7763895 |
| bovine adrenodoxin--a mitochondrial iron-sulphur protein--binds to chaperonin groel. | the interaction of bovine adrenodoxin with the chaperonin groel was investigated using sucrose density centrifugation and analytical ultracentrifugation. it could be clearly established that denatured mature adrenodoxin comigrated in a sucrose density gradient with the groel oligomer, indicating that a complex had been formed. up to 2 moles of adrenodoxin/mol groel can be bound. from the partial concentrations, association constants of 4.3 x 10(5) m-1 for the first adrenodoxin molecule and of 1. ... | 1995 | 7763228 |
| a native plasmid of pasteurella haemolytica serotype a1: dna sequence analysis and investigation of its potential as a vector. | the complete nucleotide sequence of a 4.3 kilobase pair plasmid, pab2, isolated from a bovine strain of pasteurella haemolytica serotype a1, was determined. it encodes a rob-1 type beta-lactamase and a region with homology to the mobilisation (mob) region of the escherichia coli plasmid, cole1. an insertion mutant of pab2 (ptc2/81) carrying a copy of tn5 was transferred to e coli k12 by conjugation. subsequently ptc2/81 could be transferred by transformation to e coli hb101, but not to p haemoly ... | 1995 | 7761696 |
| overexpression and characterization of human tetrameric pyruvate dehydrogenase and its individual subunits. | pyruvate dehydrogenase (e1), an alpha 2 beta 2 tetramer, is the first component of the pyruvate dehydrogenase complex which catalyzes a two-step oxidative decarboxylation of pyruvic acid. to overexpress human e1 and its subunits individually, cdnas for the mature forms of human e1 alpha and e1 beta were subcloned either individually or together into a plasmid pqe-9 and expressed in escherichia coli m15. a polyhistidine extension was added at the nh2-termini of the recombinant e1 alpha and e1 bet ... | 1995 | 7756842 |
| cloning, sequencing and bacterial expression of human glycine trna synthetase. | the human glycine trna synthetase gene (glyrs) has been cloned and sequenced. the 2462 bp cdna for this gene contains a large open reading frame (orf) encoding 685 amino acids with predicted m(r) = 77,507 da. the protein sequence has approximately 60% identity with b. mori glyrs and 45% identity with s. cerevisiae glyrs and contains motifs 2 and 3 characteristic of class ii trna synthetases. a second orf encoding 47 amino acids is found upstream of the large orf. translation of this orf may prec ... | 1995 | 7753621 |
| characterization of an operon encoding an nadp-reducing hydrogenase in desulfovibrio fructosovorans. | a genomic dna fragment from desulfovibrio fructosovorans, which strongly hybridized with the hydab genes from desulfovibrio vulgaris hildenborough, was cloned and sequenced. this fragment was found to contain four genes, named hnda, hndb, hndc, and hndd. analysis of the sequence homologies indicated that hnda shows 29, 21, and 26% identity with the 24-kda subunit from bos taurus complex i, the 25-kda subunit from paracoccus denitrificans nadh dehydrogenase type i, and the n-terminal domain of ho ... | 1995 | 7751270 |
| applications of molecular biology to study aldehyde dehydrogenase. | liver mitochondrial aldehyde dehydrogenase has been cloned and expressed in e. coli as the mature or the precursor form. this has allowed for site directed mutagenesis to be performed to study active site components of the enzyme. residues investigated were cysteine, histidines and a glutamate. the import of the precursor into mitochondria in the presence and absence of added alcohols was investigated. alcohols, in a dose dependent manner, inhibited the import of the precursor into isolated rat ... | 1993 | 7748349 |
| effects of an escherichia coli j5 vaccine on mild clinical coliform mastitis. | efficacy of an escherichia coli (o111:b4) j5 bacterin was tested in an experimental challenge trial. nineteen cows were vaccinated with an e. coli j5 bacterin, and 10 cows were injected with a placebo containing adjuvant only. vaccine and placebo were administered at drying off, 30 d after drying off, and within 48 h after calving. cows were challenged approximately 30 d after calving by intramammary infusion with a smooth heterologous strain of e. coli previously shown to cause mild clinical ma ... | 1995 | 7745148 |
| isolation of a high affinity inhibitor of urokinase-type plasminogen activator by phage display of ecotin. | ecotin, a serine protease inhibitor found in the periplasm of escherichia coli, is unique in its ability and mechanism of inhibiting serine proteases of a broad range of substrate specificity. however, although the catalytic domain of human urokinase-type plasminogen activator (upa) has 40% identity to bovine trypsin and the substrate specificities of these two proteases are virtually identical, ecotin inhibits upa almost 10,000-fold less efficiently than trypsin. ecotin was expressed on the sur ... | 1995 | 7744876 |
| apparent inhibition of superoxide dismutase activity in vitro by diesel exhaust particles. | the inhibitory effects of diesel exhaust particles (dep) on superoxide dismutase (sod) activity were examined in vitro because intratracheal administration of dep to mice resulted in a suppression of the pulmonary enzyme activity (sagai et al., free radic. biol. med. 14:37-47; 1993). superoxide production, based on the reduction of cytochrome c, was suppressed considerably by the soluble fraction of mouse lung and by purified sod from bovine erythrocytes, but the suppression was drastically dimi ... | 1995 | 7744321 |
| processing of chromogranin b in bovine adrenal medulla. identification of secretolytin, the endogenous c-terminal fragment of residues 614-626 with antibacterial activity. | chromogranins constitute a family of acidic soluble proteins widely distributed in endocrine cells and neurons. chromogranin a, the major soluble component in bovine adrenal medullary secretory granules in chromaffin cells, has been shown to be actively processed to peptide fragments [metz-boutigue, m. h., garcia-sablone, p., hogue-angeletti, r. & aunis, d. (1993) eur. j. biochem. 217, 247-257]. in the present paper, the structural features of the proteolytic degradation mechanism of chromograni ... | 1995 | 7744058 |
| the impact of infection on gluconeogenesis in the conscious dog. | the effect of infection on gluconeogenesis was assessed in the chronically catheterized conscious dog. dogs were studied 42 h after implantation of a sterile (n = 7) or an escherichia coli containing (n = 7) fibrinogen clot into the peritoneum (54 h fasted). infection increased arterial plasma glucagon and cortisol (4.2- and 2.1-fold, respectively), but it did not alter arterial plasma insulin, catecholamines, or glucose concentrations. infection increased tracer ([3-3h]glucose) determined gluco ... | 1994 | 7743359 |
| neutralizing antibodies against shiga-like toxins from escherichia coli in colostra and sera of cattle. | previous or present infection with shiga-like toxin producing e. coli (sltec) was detected by an indirect neutralization assay of antibody titer. bovine colostra and sera blocked the cytotoxic effects of shiga-like toxin on vero cell monolayers. slt neutralizing antibodies were present in 84.0% (189/225) of the colostrum samples from randomly chosen cows in bavaria, germany. while all of the colostra with neutralizing activity reacted with slt-i, only 14.7% neutralized both slt-i and -ii. approx ... | 1995 | 7740752 |
| targeting phosphodiesterases as a strategy for killing tumor cells. | ribonucleases (rnases) are being employed as alternative cytotoxic proteins to the conventionally used ones such as ricin and pseudomonas exotoxin. mammalian rnases are attractive enzymes because of their comparable cytotoxicity when suitably directed and the likelihood of lower immunogenicity compared to plant and bacterial toxins. bovine seminal rnase (bsrnase) is a member of the rnase superfamily, but differs in many interesting ways. unlike the rest of the family it is dimeric, and possesses ... | 1994 | 7736530 |
| cloning and expression of a catalytic core bovine brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. | a cdna encoding the catalytic core of a novel brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoenzyme was isolated from a lambda gt10 bovine brain library. this brain cdna begins and ends in an open reading frame encoding a peptide of 476 amino acids. this peptide contains both the catalytic kinase and bisphosphatase domains and has an overall 65% and 67% indentity with the bovine heart and liver isozymes, respectively, whereas the nh2 and cooh-termini are divergent. an active catal ... | 1995 | 7733968 |
| escherichia coli o157:h7: clinical, diagnostic, and epidemiological aspects of human infection. | e. coli o157:h7 is one of many e. coli organisms that contain genes encoding one or more toxins similar in structure and function to shiga toxin. e. coli o157:h7 is the most frequently isolated diarrheagenic type of e. coli isolated in north america today; this pathogen can cause serious, even fatal disease. syndromes caused by e. coli o157:h7 include diarrhea, hemorrhagic colitis, and hus. poorly cooked ground beef has been the most frequently implicated vehicle of transmission, but additional ... | 1995 | 7727633 |
| solution structure of a low molecular weight protein tyrosine phosphatase. | protein tyrosine phosphatases (ptps) are important enzymes involved in signal transduction, cell cycle regulation, and the control of differentiation. despite the importance of this class of enzymes in the control of critical cell processes, very little structural information is available for this family of proteins. in this paper, we present the first solution structure of a protein tyrosine phosphatase. this protein is a low molecular weight cytosolic ptp that was initially isolated from bovin ... | 1994 | 7727361 |
| dna polymerase delta holoenzyme: action on single-stranded dna and on double-stranded dna in the presence of replicative dna helicases. | dna polymerase delta requires proliferating cell nuclear antigen and replication factor c to form a holoenzyme efficient in dna synthesis. we have analyzed three different aspects of calf thymus dna polymerase delta holoenzyme: (i) analysis of pausing during dna synthesis, (ii) replication of double-stranded dna in the absence of additional factors, and (iii) replication of double-stranded dna in the presence of the two known replicative dna helicases from simian virus 40 and bovine papilloma vi ... | 1995 | 7711022 |
| molecular characterisation of escherichia coli o157:h7 isolates by pulsed-field gel electrophoresis and plasmid dna analysis. | foods of bovine origin have been identified as sources of escherichia coli o157:h7. genomic dna of e. coli o157:h7 isolates from patients (six isolates), food samples (18 isolates from ground beef and six isolates from raw milk) and calf faecal samples (31 isolates) were characterised by pulsed-field gel electrophoresis (pfge) and plasmid dna analysis. these isolates originated from different locations in the usa during 1992 and 1993. twenty-one distinct genomic profiles were generated from the ... | 1995 | 7707333 |
| the crystal structure of recombinant human neutrophil-activating peptide-2 (m6l) at 1.9-a resolution. | neutrophil-activating peptide-2 (nap-2) is a 70-residue carboxyl-terminal fragment of platelet basic protein, which is found in the alpha-granules of human platelets. nap-2, which belongs to the cxc family of chemokines that includes interleukin-8 and platelet factor 4, binds to the interleukin-8 type ii receptor and induces a rise in cytosolic calcium, chemotaxis of neutrophils, and exocytosis. crystals of recombinant nap-2 in which the single methionine at position 6 was replaced by leucine to ... | 1995 | 7706245 |
| expression, purification and immunologic analysis of three pasteurella haemolytica a1 28-30 kda lipoproteins. | three genes, tandemly arranged on the pasteurella haemolytica a1 chromosome and encoding similar 28-30 kda proteins, were previously cloned and sequenced by our laboratory. in this study, we demonstrate that the cloned genes encode lipoproteins, as previously suggested by dna sequence analysis. to further analyze the bovine immune response to these proteins, the individual genes were cloned separately into an expression vector and recombinant forms of the three proteins were purified after expre ... | 1994 | 7700132 |
| expression in escherichia coli, purification and functional activity of recombinant human chaperonin 10. | we have recently reported the cloning of a cdna coding for a stress inducible human chaperonin 10. the protein was shown to possess 100% identity with the bovine homologue and a single amino acid replacement (glycine to serine at position 52) compared to rat chaperonin 10. here we report the heterologous expression of human chaperonin 10 in escherichia coli, its purification and its functional characterization. the recombinant protein was purified to homogeneity as judged by different analytical ... | 1995 | 7698325 |
| anti-alpha-galactosyl immunoglobulin a (iga), igg, and igm in human secretions. | anti-alpha-galactosyl (anti-gal) is a natural human serum antibody that binds to the carbohydrate gal alpha 1,3gal beta 1,4glcnac-r (alpha-galactosyl epitope) and is synthesized by 1% of circulating b lymphocytes in response to immune stimulation by enteric bacteria. we were able to purify secretory anti-gal from human colostrum and bile by affinity chromatography on silica-linked gal alpha 1,3gal beta 1,4glcnac. we found similar secretory anti-gal antibodies in human milk, saliva, and vaginal w ... | 1995 | 7697518 |
| in vitro detection of bovine immunodeficiency-like virus using monoclonal antibodies generated to a recombinant gag fusion protein. | an escherichia coli recombinant fusion protein containing the major core protein of bovine immunodeficiency-like virus (biv) was used to immunize mice for generation of monoclonal antibodies to biv p26. eight hybridomas specific for biv p26 were identified and two antibodies, designated 104 and 142, were further characterized. both 104 and 142 antibodies were isotyped as igg1; they reacted specifically with both biv p26 and the recombinant fusion protein in western immunoblot analyses. however, ... | 1993 | 7693743 |
| [enterotoxigenic escherichia coli k99+, serotype 08:k25, produce cytotoxic necrotizing factor cnf1 and alpha-hemolysin]. | a total of 54 k99+ and/or f41+ escherichia coli strains isolated of calves and piglets with diarrhoea in different countries were investigated for production of heat-labile (lt) and heat-stable (sta) enterotoxins, verotoxins (vt1 and vt2), cytotoxic necrotizing factors (cnf1 and cnf2), alpha-haemolysin (hly) and enterohaemolysin (enthly). fifty-one (94%) strains were sta+, two (4%) were sta+cnf1+hly+ and one (2%) was not toxigenic. the two sta+cnf1+hly+ e. coli strains expressed the k99 intestin ... | 1993 | 7691086 |
| bovine seminal ribonuclease produced from a synthetic gene. | bovine seminal ribonuclease (bs-rnase), a homolog of bovine pancreatic ribonuclease (rnase a), is isolated as a dimer in which the subunits are cross-linked by two disulfide bonds. in addition to this anomalous quaternary structure, the enzyme has extraordinary biological properties, such as antispermatogenic, antitumor, and immunosuppressive activities. the molecular bases for these properties are well-suited for exploration with the techniques of recombinant dna. accordingly, a gene encoding b ... | 1993 | 7688724 |
| polymerization of 70-kda heat shock protein by yeast dnaj in atp. | dnak, the escherichia coli hsp70 protein, interacts with dnaj, a protein cofactor that appears to be involved in presenting protein substrates to dnak. the yeast dnaj homolog, ydj1, has also been shown to interact with yeast hsp70, although the function of this interaction is unknown. in the present study, we investigated the interaction of ydj1 with both yeast and bovine brain hsp70. we found that, in the presence of atp, where hsp70 is normally monomeric, ydj1 induced almost all of the yeast a ... | 1995 | 7673245 |
| nucleotide sequences of two fimbrial major subunit genes, pmpa and ucaa, from canine-uropathogenic proteus mirabilis strains. | proteus mirabilis strains were isolated from dogs with urinary tract infection (uti) and fimbriae were prepared from two strains. the n-terminal amino acid sequences of the major fimbrial subunits were determined and both sequences appeared identical to the n-terminal amino acid sequence of a urinary cell adhesin (uca) (wray, s. k., hull, s. i., cook, r. g., barrish, j. & hull, r. a., 1986, infect immun 54, 43-49). the genes of two different major fimbrial subunits were cloned using oligonucleot ... | 1995 | 7670636 |
| interaction of alpha-lipoic acid enantiomers and homologues with the enzyme components of the mammalian pyruvate dehydrogenase complex. | lipoic acid (alpha-lipoic acid, thioctic acid) is applied as a therapeutic agent in various diseases accompanied by polyneuropathia such as diabetes mellitus. the stereoselectivity and specificity of lipoic acid for the pyruvate dehydrogenase complex and its component enzymes from different sources has been studied. the dihydrolipoamide dehydrogenase component from pig heart has a clear preference for r-lipoic acid, a substrate which reacts 24 times faster than the s-enantiomer. selectivity is m ... | 1995 | 7669066 |
| oxidative damage to 5-methylcytosine in dna. | exposure of pyrimidines of dna to ionizing radiation under aerobic conditions or oxidizing agents results in attack on the 5,6 double bond of the pyrimidine ring or on the exocyclic 5-methyl group. the primary product of oxidation of the 5,6 double bond of thymine is thymine glycol, while oxidation of the 5-methyl group yields 5-hydroxymethyluracil. oxidation of the 5,6 double bond of cytosine yields cytosine glycol, which decomposes to 5-hydroxycytosine, 5-hydroxyuracil and uracil glycol, all o ... | 1995 | 7667100 |
| cloning, expression, and purification of rat brain protein l-isoaspartyl methyltransferase. | protein l-isoaspartyl methyltransferase (pimt) methylates isoaspartyl residues in peptides and proteins using s-adenosyl-l-methionine as the methyl donor. a cloned source of this enzyme should be useful in the identification of cellular substrates and for quantitation and localization of isoaspartyl sites in purified proteins, including recombinant proteins used as pharmaceuticals. rat brain pimt cdna was amplified using the polymerase chain reaction. the reaction product was directionally clone ... | 1995 | 7663167 |
| [the presence of escherichia coli o157:h7 in healthy cattle]. | 1995 | 7660091 | |
| clinical pharmacology of cefixime in unweaned calves. | cefixime is a unique third-generation oral cephalosporin. its in vitro activity and pharmacokinetic properties have been studied to assess its potential for use in the therapy of newborn calf infections due to gram-negative bacteria. the minimum inhibitory concentrations of cefixime for 90% (mic90) of field isolates of escherichia coli, salmonella and pasteurella were 0.10-0.40 micrograms/ml. the serum disposition kinetics of cefixime following intravenous and oral administration was evaluated. ... | 1995 | 7629935 |
| expression and characterization of the 66-kilodalton (nqo3) iron-sulfur subunit of the proton-translocating nadh-quinone oxidoreductase of paracoccus denitrificans. | the proton-translocating nadh-quinone oxidoreductase (ndh-1) of paracoccus denitrificans is composed of at least 14 dissimilar subunits which are designated nqo1-14 and contains one noncovalently bound fmn and at least five epr-visible iron-sulfur clusters (n1a, n1b, n2, n3, and n4) as prosthetic groups. comparison of the deduced primary structures of the subunits with consensus sequences for the cofactor binding sites has predicted that nqo1, nqo2, nqo3, nqo9, and probably nqo6 subunits are cof ... | 1995 | 7629145 |
| identification and characterization of is1296 in mycoplasma mycoides subsp. mycoides sc and presence in related mycoplasmas. | is1296, a new insertion sequence belonging to the is3 family of insertion elements has been identified in mycoplasma mycoides subsp. mycoides (mmm) biotype small colony (sc), the agent of contagious bovine pleuropneumonia (cbpp). is1296 is 1485-bp long and has 30-bp inverted repeats. it contains two open reading frames, orfa and orfb, which show significant similarities to the orfs which encode the transposase function of is elements of the is3 family, in particular is150 of escherichia coli. is ... | 1995 | 7628725 |
| immunochemical localization of calcium/calmodulin-dependent protein kinase i. | ca2+/calmodulin-dependent protein kinase i (cam kinase i) was originally identified in rat brain based on its ability to phosphorylate site 1 of synapsin i. recently a cdna for the rat brain enzyme has been cloned and the primary structure elucidated [picciotto et al. (1993), j. biol. chem., 268:26512-26521]. the rat cdna encoded a protein of 374 amino acids with a calculated m(r) of 41,636. antibodies have now been raised against the recombinant kinase expressed in e. coli as a glutathione-s-tr ... | 1995 | 7624832 |
| a novel bacteristatic action of bovine and porcine serum that is reversed by norepinephrine. | a previously unreported anti-bacterial activity against escherichia coli has been found in porcine and bovine sera, but not in human or chicken sera. the activity is not affected by heat inactivation of the complement system, and is bacteriostatic rather than bactericidal. growth inhibition is reversible by addition of norepinephrine. | 1995 | 7623610 |
| interaction between cardiolipin and the mitochondrial presequence of cytochrome c oxidase subunit iv favours lipid mixing without destabilizing the bilayer structure. | we demonstrate the ability of a peptide corresponding to the presequence of the cytochrome c oxidase subunit iv to induce lipid mixing between large unilamellar liposomes. this lipid mixing requires the presence of cl or pe, lipids able to form non-bilayer structures, and is not observed with other negatively charged lipids. however, the fact that this mixing occurs without mixing of the liposome aqueous phases and without destabilizing the lipid organisation is unusual and has not been observed ... | 1995 | 7615071 |
| thermostability of respiratory terminal oxidases in the lipid environment. | the effect of the lipid environment on the thermostability of three respiratory terminal oxidases was determined. cytochrome-c oxidase from beef heart and bacillus stearothermophilus were used as representative proteins from mesophilic and thermophilic origin, respectively. quinol oxidase from the archaeon sulfolobus acidocaldarius represented the model for a extreme thermoacidophilic enzyme. all three integral membrane proteins were tested for their thermal inactivation in detergent and after r ... | 1995 | 7612641 |
| [udder disinfection and mastitis in cattle: a literature review]. | postmilking teat disinfection is accepted as an important part of standard preventive measures against mastitis in dairy cattle. the efficacy of postmilking teat disinfection against infections with contagious pathogens such as staphylococcus aureus and streptococcus agalactiae is beyond doubt. however, the efficacy of teat disinfection against infections with environmental pathogens such as escherichia coli is disputed, and a negative effect has even been described in some situations. this arti ... | 1995 | 7610449 |
| endogenous tumor necrosis factor (tnf) production and modification of pathological lesions in experimental escherichia coli endotoxemia of piglets. | we examined the kinetics of tumor necrosis factor (tnf) production induced by escherichia coli lipopolysaccharide (lps) in relation to lps tolerance and endotoxemic lesions of piglets. the plasma of piglets demonstrated cytotoxicity to tnf-sensitive l929 cells between 0.5 and 4 h after inoculation with 200 micrograms kg-1 of lps. this cytotoxicity was neutralized by anti-bovine tnf serum. these piglets had disseminated intravascular coagulation (dic) and meningoencephalitis. however, if piglets ... | 1995 | 7604537 |
| immunological response to recombinant vp8* subunit protein of bovine roravirus in pregnant cattle. | bovine rotavirus vp8*, n-terminal trypsin cleavage product of vp4, was produced in escherichia coli. to examine if this antigen could induce neutralizing antibody responses, different species of animals were immunized with the recombinant vp8* protein. the vp8* antigen was found to stimulate a neutralizing immune response in rabbits. when vp8*-immunized mice were exposed to bovine rotavirus strain c486, significantly higher antibody responses were observed than if they were only exposed to c486. ... | 1995 | 7595351 |
| cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase. | the cdna encoding qpc-9.5 kda (subunit vii) of bovine heart mitochondrial ubiquinol-cytochrome c reductase was cloned and sequenced. this cdna is 665 base pairs long with an open reading frame of 246 base pairs that encodes an 81-amino acid mature qpc-9.5 kda. the insert contains 395 base pairs of a 3'-noncoding sequence with a poly(a) tail. the amino acid sequence of qpc-9.5 kda deduced from this nucleotide sequence is the same as that obtained by protein sequencing except that residue 61 is tr ... | 1995 | 7592738 |
| molecular cloning and deduced amino acid sequences of the alpha- and beta- subunits of mammalian nad(+)-isocitrate dehydrogenase. | a 153 bp fragment of the cdna encoding the beta-subunit of pig heart nad(+)-isocitrate dehydrogenase (nad(+)-icdh) was specifically amplified by pcr, using redundant oligonucleotide primers based on partial peptide sequence data [huang and colman (1990) biochemistry 29, 8266-8273]. this pcr fragment was then used as a probe to isolate cdna clones encoding the complete mature form of the beta-subunit from a monkey testis cdna library. examination of the deduced amino acid sequence of the monkey s ... | 1995 | 7575427 |
| suitability of selective plating media for recovering heat- or freeze-stressed escherichia coli o157:h7 from tryptic soy broth and ground beef. | the efficacy of tryptic soy agar (tsa), modified sorbitol macconkey agar (msma), modified eosin methylene blue (memb) agar, and modified sd-39 (msd) agar in recovering a five-strain mixture of enterohemorrhagic escherichia coli o157:h7 and five non-o157 strains of e. coli heated in tryptic soy broth at 52, 54, or 56 degrees c for 10, 20, and 30 min was determined. nonselective tsa supported the highest recovery of heated cells. significantly (p < or = 0.05) lower recovery of heat-stressed cells ... | 1995 | 7574637 |
| investigations into the mechanism of toxicity of lipopolysaccharide (lps) in bovine aortic endothelial cells. | the cytotoxic effect of lipopolysaccharide (lps) was examined on bovine aortic endothelial cell proliferation in vitro. these lps-induced cytotoxicity (ic50 = 20 ng/ml) was not inhibitable by substances regulating the formation of nitric oxide (no). e.g. by ng-monomethyl-l-arginine (l-nmma), an inhibitor of no synthesis, and by the glucocorticoid dexamethasone, an inhibitor of the induction of no synthase. also other substances which inhibit the generation or action of oxygen radicals, as glutat ... | 1995 | 7568318 |
| a role for phosphatidylinositol transfer protein in secretory vesicle formation. | vesicular traffic in eukaryotic cells is characterized by two steps of membrane rearrangement: the formation of vesicles from donor membranes and their fusion with acceptor membranes. with respect to vesicle formation, several of the cytosolic proteins implicated in budding and fission have been identified. a feature common to all these proteins is that their targets, when known, are other proteins rather than lipids. here we report, using a previously established cell-free system derived from a ... | 1995 | 7566155 |
| a residue to residue hydrogen bond mediates the nucleotide specificity of ribonuclease a. | bovine pancreatic ribonuclease a (rnase a) catalyzes the cleavage of the p-o5 bond of rna after residues bound in the enzyme's b1 subsite. this subsite binds to cytidine 30-fold more tightly than to uridine and > 10(5)-fold more tightly than to adenine. structural studies had suggested that the hydroxyl group of thr45 can interact directly with the base of a bound nucleotide. in contrast, the carboxylate group of asp83 cannot interact directly with bound substrate but can accept a hydrogen bond ... | 1995 | 7563054 |
| a novel function of escherichia coli chaperone dnaj. protein-disulfide isomerase. | molecular chaperones, protein-disulfide isomerases, and peptidyl prolyl cis-trans isomerases assist protein folding in both prokaryotes and eukaryotes. the dnaj protein of escherichia coli and the dnaj-like proteins of eukaryotes are known as molecular chaperones and specific regulators of dnak-like proteins and are involved in protein folding and renaturation after stress. in this study we show that dnaj, like thioredoxin, protein-disulfide isomerase, and dsba, possesses an active dithiol/disul ... | 1995 | 7559385 |
| myositis, lameness, and paraparesis associated with use of an oil-adjuvant bacterin in beef cows. | right hind limb lameness, progressing to bilateral paraparesis, was observed in 56 of 610 (9%) beef cows. lameness began 6 days to 4 weeks after vaccination in the right longissimus lumborum (loin) muscle with an escherichia coli/campylobacter bacterin in an oil adjuvant. postmortem examination of 5 affected cows revealed a large inflammatory mass at the site of vaccination. in each cow, the mass spread through adjacent intervertebral foramina into the vertebral canal and compressed the lumbar p ... | 1995 | 7559029 |
| sequence, molecular properties, and chromosomal mapping of mouse lumican. | lumican is a major proteoglycan of vertebrate cornea. this study characterizes mouse lumican, its molecular form, cdna sequence, and chromosomal localization. | 1995 | 7558724 |
| potential role of nitric oxide in the pathophysiology of experimental bacterial meningitis in rats. | we have investigated the possible role of nitric oxide (no) in the pathophysiology of bacterial meningitis (bm) by using the rat model of experimental bm. the nitrite concentration in cerebrospinal fluid (csf) was used as a measure of no production in vivo since no rapidly degrades to nitrite and nitrate. rats were inoculated intracisternally with live bacteria (5 x 10(6) cfu of haemophilus influenzae type b strain dl42 or rd-/b+/o2), with bacterial endotoxin (20 ng of dl42 lipooligosaccharide [ ... | 1995 | 7558288 |
| casocidin-i: a casein-alpha s2 derived peptide exhibits antibacterial activity. | here we report the isolation and characterization of an antibacterial peptide from bovine milk inhibiting the growth of escherichia coli, and staphylococcus carnosus. the primary structure of the peptide was revealed as a 39-amino-acid-containing fragment of bovine alpha s2-casein (position 165-203) by means of edman amino acid sequencing and mass spectrometry. since human milk does not contain any casein-alpha s2, these findings could explain the different influence of human and bovine milk on ... | 1995 | 7556666 |
| [enterotoxigenic, verotoxigenic, and necrotoxigenic escherichia coli in food and clinical samples. role of animals as reservoirs of strains pathogenic for humans]. | toxigenic escherichia coli of human and animal origin have been classified into three categories: enterotoxigenic e. coli (etec), verotoxigenic e. coli (vtec), and necrotoxigenic e. coli (ntec), etec are a major cause of infant diarrhoea in less-developed countries and frequently cause colibacillosis in domestic animals. human etec strains may synthesize lt-i and/or sta enterotoxins and they may possess the colonization factors cfa/i to cfa/iv; porcine strains synthesize lt-i, sta and/or stb, an ... | 1995 | 7546450 |
| stereospecificity of human dna polymerases alpha, beta, gamma, delta and epsilon, hiv-reverse transcriptase, hsv-1 dna polymerase, calf thymus terminal transferase and escherichia coli dna polymerase i in recognizing d- and l-thymidine 5'-triphosphate as substrate. | l-beta-deoxythymidine (l-dt), the optical enantiomer of d-beta-deoxythymidine (d-dt), and l-enantiomers of nucleoside analogs, such as 5-iodo-2'-deoxy-l-uridine (l-idu) and e-5-(2-bromovinyl)-2'-deoxy-l-uridine (l-bvdu), are not recognized in vitro by human cytosolic thymidine kinase (tk), but are phosphorylated by herpes simplex virus type 1 (hsv-1) tk and inhibit hsv-1 proliferation in infected cells. here we report that: (i) l-dt is selectively phosphorylated in vivo to l-dtmp by hsv-1 tk and ... | 1995 | 7544886 |
| diffusion-limited interaction between unfolded polypeptides and the escherichia coli chaperone secb. | secb is a chaperone dedicated to protein translocation in escherichia coli. secb binds to a subset of precursor proteins, and targets them in a translocation-competent state to the seca subunit of the translocase. the nature and kinetics of the interaction of secb with polypeptides were studied by spectroscopic techniques using the reduced form of bovine pancreatic trypsin inhibitor (bpti) as a model substrate. binding of secb to bpti resulted in an increase in the fluorescence of the surface-ex ... | 1995 | 7543278 |
| recognition by viral and cellular dna polymerases of nucleosides bearing bases with nonstandard hydrogen bonding patterns. | the ability of dna polymerases (pols) to catalyze the template-directed synthesis of duplex oligonucleotides containing a nonstandard watson-crick base pair between a nucleotide bearing a 5-(2,4-diaminopyrimidine) heterocycle (d kappa) and a nucleotide bearing either deoxyxanthosine (dx) or n1-methyloxoformycin b (pi) has been investigated. the kappa-x and kappa-pi base pairs are jointed by a hydrogen bonding pattern different from and exclusive of those joining the at and gc base pairs. reverse ... | 1995 | 7541538 |
| virulence markers of shiga-like toxin-producing escherichia coli strains originating from healthy domestic animals of different species. | shiga-like toxin (verotoxin)-producing strains of escherichia coli (sltec) originating from healthy cattle, sheep, goats, pigs, cats, and dogs were investigated for properties which are related to virulence of e. coli for humans. the slt-ii (shiga-like toxin ii) and slt-iic genes were frequent in sltec from healthy cattle and dogs but were rarely found in sltec from other animals. the slt-iie gene was detected only in porcine sltec. sltec from goats and sltec from sheep were found to carry diffe ... | 1995 | 7538509 |
| characterization of gangliosides of epithelial cells of calf small intestine, with special reference to receptor-active sequences for enteropathogenic escherichia coli k99. | glycolipids were prepared from epithelial cells of the small intestine of a newborn calf and assayed for escherichia coli k99 binding activity on thin-layer chromatograms and in microtiter wells. the bacteria did not bind to any of the non-acid glycolipids, while in the acid fraction several binding-positive glycolipids were detected. the acid glycolipids were isolated and characterized by mass spectrometry, proton nmr spectroscopy and other methods. the following gangliosides were identified, m ... | 1994 | 7531686 |
| src-homology 2 (sh2) domain ligation as an allosteric regulator: modulation of phosphoinositide-specific phospholipase c gamma 1 structure and activity. | phosphoinositide-specific phospholipase c gamma 1 (pi-plc gamma 1) catalyses the hydrolysis of ptdins(4,5)p2 to generate the second messengers diacylglycerol and ins(1,4,5)p3. pi-plc gamma 1, an src-homology 2/3 (sh2/sh3)-domain-containing enzyme, is activated in response to growth-factor-induced tyrosine phosphorylation, and, in vivo, is translocated from the cytosol to the particulate cell fraction. here we report the bacterial expression of rat brain pi-plc gamma 1 under the control of the t7 ... | 1995 | 7531435 |
| [study of the transmembrane organization of the c-terminal domain of the alpha subunit of na+,k+-atpase using polyclonal antibodies to its perimembrane fragments]. | the fragments 825-842, 868-886, 928-945, 946-967, 962-978 of the alpha-subunit of the porcine na+,k(+)-atpase were expressed in e. coli as fusion proteins with human tumour necrosis factor. polyclonal antibodies were obtained to these fragments. the location of the chosen fragments according to the plasma membrane was determined with the polyclonal antibodies by elisa on intact, lysed or solubilized pig kidney embryo cells. the fragments 825-842, 928-945, and 962-978 contained intracellular epit ... | 1994 | 7530006 |
| conjugation of recombinant reverse transcriptase of hiv-1 to beta-d-galactosidase from escherichia coli for ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) of anti-hiv-1 igg. | recombinant reverse transcriptase (rt) of hiv-1 was conjugated to beta-d-galactosidase from escherichia coli in three different ways. maleimide groups were introduced into beta-d-galactosidase molecules using n,n'-o-phenylenedimaleimide in the absence (method i) or presence (method ii) of n-ethylmaleimide or into beta-d-galactosidase molecules, which had been treated with excess of 4,4'-dithiodipyridine to block thiol groups, using n-succinimidyl-6-maleimidohexanoate (method iii). subsequently, ... | 1994 | 7518483 |
| interaction of immobilized recombinant mouse c-type macrophage lectin with glycopeptides and oligosaccharides. | inflammatory and tumoricidal macrophages express galactose- and n-acetylgalactosamine-specific ca(2+)-dependent lectins on their surfaces. this lectin is a family member of membrane-bound c-type animal lectins and consists of 304 amino acid residues (molecular weight 34,595). in the present study, expression vectors containing a nucleotide sequence corresponding to the carbohydrate-binding domain of mouse macrophage lectin cdna have been prepared. the carbohydrate-binding specificity of the reco ... | 1994 | 7517698 |
| three distinct human thymopoietins are derived from alternatively spliced mrnas. | thymopoietin (tp) was originally isolated as a 5-kda 49-aa protein from bovine thymus in studies of the effects of thymic extracts on neuromuscular transmission and was subsequently observed to affect t-cell differentiation and function. we now report the isolation of cdna clones for three alternatively spliced mrnas that encode three distinct human t-cell tps. proteins encoded by these mrnas, which we have named tp alpha (75 kda), tp beta (51 kda), and tp gamma (39 kda), contain identical n-ter ... | 1994 | 7517549 |
| interactions between fluoroquinolones, mg2+, dna and dna gyrase, studied by phase partitioning in an aqueous two-phase system and by affinity chromatography. | the primary target of fluoroquinolones has been identified as the enzyme dna gyrase, but the mechanism of action of these antibacterial agents is still a matter of controversy. using partitioning in aqueous polyethylene glycol (peg)-dextran systems, the affinities of several fluoroquinolones for dna were determined with accuracy and at equilibrium. it was proved that the binding was strongly dependent on the ability of the drugs to bind mg2+, with ka values of about 40 000 l mol-1, but was poorl ... | 1994 | 7516247 |
| cs31a capsule-like antigen as an exposure vector for heterologous antigenic determinants. | cs31a is a multimeric surface protein surrounding certain enterotoxigenic and septicemic bovine escherichia coli strains. the possibility of using cs31a as a carrier of foreign antigenic determinants was investigated. introduction of heterologous viral epitopes into the cs31a major subunit, clpg, was successfully performed in the v3 region of the molecule which encodes for an immunodominant linear epitope. e. coli k-12 strains producing the hybrid cs31a molecules or the purified chimeric protein ... | 1994 | 7514578 |
| effects of dsba on the disulfide folding of bovine pancreatic trypsin inhibitor and alpha-lactalbumin. | dsba is a protein found in the periplasm of escherichia coli that is required for the formation of disulfide bonds in secreted proteins. it contains only two cysteine residues, which can form reversibly a very unstable disulfide bond that has been proposed to be the oxidant that introduces disulfide bonds into secreted proteins. the present study investigates the effect of dsba on the well-characterized disulfide-coupled refolding processes of bpti and of alpha-lactalbumin. disulfide-bonded dsba ... | 1994 | 7513556 |
| characterization of a linear epitope on chlamydia trachomatis serovar l2 dnak-like protein. | a cytoplasmic 75-kda immunogen from chlamydia trachomatis serovar l2 has previously been characterized as being similar to the escherichia coli heat shock protein dnak. we have localized a linear epitope for one monoclonal antibody specific for c. trachomatis dnak. by use of a recombinant dna technique, the epitope was limited to 14 amino acids. with synthetic peptides, the epitope was further limited to eight amino acids. six of these amino acids are conserved in bovine hsp70, which has a known ... | 1994 | 7513310 |
| molecular characterization of dog albumin as a cross-reactive allergen. | indoor allergens comprise a group of allergenic proteins that are commonly derived from house dust mite and cat and dog dander. in addition to the two major dog allergens (molecular weights: 19 and 23 kd), dog albumin represents an important allergen for up to 35% of patients who are allergic to dogs. in ige immunoblot inhibition studies and histamine release tests it has been demonstrated that patients who react to dog albumin exhibit ige reactivity with purified albumins from cat, mouse, chick ... | 1994 | 7512102 |
| [antibodies against escherichia coli reca protein reveal two nuclear proteins in bovine spermatocytes which interact with synaptonemal complex structures of meiotic chromosomes of various eukaryotic organisms]. | three reca-like proteins were detected in bovine meiotic cells using antibodies against escherichia coli reca protein. after isolation and purification of these reca-like proteins their molecular weights appeared to be equal to 37, 70 and 130 kd. the 37 kd protein accompanies all the stages of spermatogenesis up to the stage of mature spermatozoa. the 70 kd protein is detectable only in nuclei of cells at the stage of prophase i of meiotic division. these reca-like proteins are involved in the f ... | 1993 | 7509766 |
| lipopolysaccharide (lps)-binding protein and soluble cd14 function as accessory molecules for lps-induced changes in endothelial barrier function, in vitro. | bacterial lps induces endothelial cell (ec) injury both in vivo and in vitro. we studied the effect of escherichia coli 0111:b4 lps on movement of 14c-bsa across bovine pulmonary artery ec monolayers. in the presence of serum, a 6-h lps exposure augmented (p < 0.001) transendothelial 14c-bsa flux compared with the media control at concentrations > or = 0.5 ng/ml, and lps (10 ng/ml) exposures of > or = 2-h increased (p < 0.005) the flux. in the absence of serum, lps concentrations of up to 10 mic ... | 1994 | 7509346 |
| proton-translocating nicotinamide nucleotide transhydrogenase. reconstitution of the extramembranous nucleotide-binding domains. | the nicotinamide nucleotide transhydrogenase of bovine mitochondria is a homodimer of monomer m(r) = 109,065. the monomer is composed of three domains, an nh2-terminal 430-residue-long hydrophilic domain i that binds nad(h), a central 400-residue-long hydrophobic domain ii that is largely membrane intercalated and carries the enzyme's proton channel, and a cooh-terminal 200-residue-long hydrophilic domain iii that binds nadp(h). domains i and iii protrude into the mitochondrial matrix, where the ... | 1995 | 7499307 |
| molecular characterization of a hamster oviduct-specific glycoprotein. | there is growing evidence that the oviduct is not a passive conduit for gamete and embryo transport but serves a function for the gametes and/or embryos. the oviductal epithelium secretes one or more specific glycoproteins that associate with the egg after ovulation. several published reports including our preliminary studies have suggested that the egg-associating glycoprotein(s) from the oviduct exists in several mammalian species including golden hamster. however, little or almost no biochemi ... | 1995 | 7492686 |
| molecular basis of antigenic variation between the glycoproteins c of respiratory bovine herpesvirus 1 (bhv-1) and neurovirulent bhv-5. | herpesvirus glycoprotein c (gc) functions as a major virus attachment protein. the gc sequence of the neurovirulent bovine herpesvirus type 5 (bhv-5) virus was determined and compared with the gc sequence of the nonneurovirulent bhv-1. alignment of the predicted amino acid sequences of bhv-1 and bhv-5 gc orfs showed that the amino-terminal third of the protein differed between the two viruses. whole or subgenomic fragments of gc coding regions from both viruses were expressed as trpe-gc fusion p ... | 1995 | 7491780 |
| recombinant human pancreatic ribonuclease produced in e. coli: importance of the amino-terminal sequence. | human pancreatic ribonuclease 1 (hrnase 1) in the mature form has been produced in e. coli using t7 expression system. the recombinant hrnase 1 protein was solubilized from the inclusion bodies, refolded in glutathione redox system, and purified through chromatographic procedures by utilizing cation-exchange and reversed-phase columns. the ribonucleolytic activity of recombinant hrnase 1 was examined on yeast rna and cytidylyl-3',5'-adenosine revealing the distinctive ribonucleolytic activity. t ... | 1995 | 7488119 |
| recombinant human dihydroorotate dehydrogenase: expression, purification, and characterization of a catalytically functional truncated enzyme. | an n-terminally truncated cdna for human dihydroorotate dehydrogenase (dhodase) was placed under the control of the inducible t7 lac promoter in a pyrimidine auxotrophic strain of escherichia coli lacking the endogenous enzyme. induction of gene expression rescued growth in media lacking exogenous pyrimidines. the recombinant enzyme was purified to homogeneity from detergent extracts of bacterial membranes by two chromatographic steps. the purity of the resulting enzyme was judged to be > 95% ba ... | 1995 | 7487077 |
| effect of phenylbutazone and flunixin meglumine on acute toxic mastitis in dairy cows. | a double-blinded randomized prospective clinical trial was designed to evaluate the effectiveness of flunixin meglumine and phenylbutazone for treatment of acute toxic mastitis in dairy cows. all cows were treated 4 times at 12-hour intervals by intramammary infusion of gentamicin (150 mg). a total of 45 dairy cows with toxic mastitis were randomly assigned to 1 of 3 treatment groups: group 1 (control), saline solution, iv; group 2, 1 g of flunixin meglumine, iv; or group 3, 4 g of phenylbutazon ... | 1995 | 7486401 |
| effects of staphylococcus aureus mastitis after endotoxin application on milk yield and composition during subsequent lactation of guinea-pigs. | the effects of staphylococcus aureus mastitis on milk yield and composition throughout subsequent lactation in lactating guinea-pigs and the role of endotoxin pretreatment on these phenomena were investigated. primiparous lactating guinea-pigs were intramammarily inoculated with sterile saline (group 1), s. aureus strain uc 6097 (group 2), or with s. aureus uc 6097 after endotoxin pretreatment (group 3). clinical signs and survival rate were monitored. during the second lactation, daily milk yie ... | 1995 | 7483905 |
| [determination of escherichia coli shiga-like toxins by means of the mtt bioassay]. | tissue culture cells' metabolism and viability are measured by the mitochondrial reduction rate of a yellow tetrazolium salt (mtt) to blue formazan crystals in the mtt-bioassay. thus the mtt-bioassay is a standardizable and reproducible bioassay for measuring cytotoxicity or cytostimulation. it is shown that the mtt-bioassay is also very suitable for determining bacterial cytotoxins using escherichia coli's shiga-like toxins as example. 177 strains of e. coli, isolated from carcasses and organs ... | 1995 | 7483864 |
| isolation of genomic and cdna clones encoding bovine poly(a) binding protein ii. | cdna clones for bovine poly(a) binding protein ii (pab ii) were isolated. their sequence predicts a protein of 32.8 kda, revising earlier estimates of molecular mass. the protein contains one putative rna-binding domain of the rnp type, an acidic n-terminal and a basic c-terminal domain. analyses of authentic pab ii were in good agreement with all predictions from the cdna sequence except that a number of arginine residues appeared to be post-translationally modified. poly(a) binding protein ii ... | 1995 | 7479061 |
| the role of tau phosphorylation in transfected cos-1 cells. | tau cdnas from each of the six human isoforms were transfected into cos-1 cells and, in every case, more than one peptide was observed. the diversity of expressed isoforms was due to different levels of tau phosphorylation. tau phosphorylation results in a decrease of the protein electrophoretic mobility. the major contribution to this mobility shift is due to the phosphorylation at the at the c-terminus of the molecule, as inferred from the expression of tau fragments. phosphorylation takes pla ... | 1995 | 7476937 |
| diffusion-in-gel enzyme-linked immunosorbent assay (dig-elisa): optimal conditions for quantitation of antibodies. | in the diffusion-in-gel enzyme-linked immunosorbent assay (dig-elisa) the quantitation of antibodies is based on their ability to form a diffusion gradient over an antigen-coated polystyrene surface. the antigen-antibody reaction is then visualized by an enzyme-conjugated anti-immunoglobulin. the enzyme-substrate reaction is finally performed by pouring a substrate-containing gel over the polystyrene surface. in this study with bovine serum albumin as antigen and a corresponding rabbit antiserum ... | 1980 | 7462651 |
| evidence of species specificity in the cytocidal effects of pasteurella haemolytica. | pasteurella haemolytica was found to have a cytotoxic effect on the peripheral blood mononuclear leukocytes of cattle, sheep, and goats, but no effect on the mononuclear leukocytes of swine, horses, or humans. in contrast, escherichia coli caused marked cell death among mononuclear leukocytes of all species tested. | 1980 | 7439998 |
| superoxide dismutase from bacillus stearothermophilus. complete amino acid sequence of a manganese enzyme. | superoxide dismutase from the thermophilic bacterium bacillus stearothermophilus is dimeric with a molecular weight of 45 487. it contains one atom manganese(iii) per dimeric molecule [brock, c. j., harris, j. i., & sato, s. (1976) j. mol. biol. 107, 175-178]. the subunits are identical, and the following primary structure comprising 203 amino acids has been determined: (formula: see text) the enzyme is identical in 60% of its residues with the escherichia coli b manganese enzyme [steinman, h. m ... | 1980 | 7397109 |
| infectious bovine keratoconjunctivitis: effects of vaccination on moraxella bovis carrier state in cattle. | a study was conducted to determine whether vaccination of cattle while they were undergoing an acute episode of infectious bovine keratoconjunctivitis (ibk), would cause vaccinated cattle to abort moraxella bovis infection sooner than nonvaccinated cattle. fourteen calves were allotted into two groups of seven calves each, and the eyes of each calf were exposed to a virulent culture of m bovis. twenty days after calves were infected and showing signs of ibk, seven calves were vaccinated with m b ... | 1980 | 7369598 |
| purification of a low molecular weight non-histone chromosomal protein. | a non-histone chromosomal proteins was extracted from rat liver chromatin with 0.35 m nacl and purified more than 2758 times to near homogeneity by hydroxyapatite, gel filtration, and phosphocellulose chromatography. the final fraction was greater than 95% pure as judged by non-denaturing gel electrophoresis. the protein, designated loosely bound non-histone chromosomal protein 1, had an observed molecular weight of 15 700. this protein was demonstrated to increase the amount of rna synthesized ... | 1980 | 7353013 |
| fluorescence-determined preferential binding of quinacrine to dna. | quinacrine complexes with native dna (calf thymus, micrococcus lysodeikticus, escherichia coli, bacillus subtilis, and colstridium perfringens) and synthetic polynucleotides (poly(da) . poly(dt), poly[d(a-t)] . poly[d(a-t)], poly(dg) . poly(dc) and poly[d(g-c)] . poly[d(g-c)]) has been investigated in solution at 0.1 m nacl, 0.05 m tris hcl, 0.001 m edta, ph 7.5, at 20 degrees c. fluorescence excitation spectra of complexes with dye concentration d = 5-30 microm and dna phosphate concentration p ... | 1981 | 7326321 |
| control of electron transfer in the cytochrome system of mitochondria by ph, transmembrane ph gradient and electrical potential. the cytochromes b-c segment. | 1. a study is presented of the effects of ph, transmembrane ph gradient and electrical potential on oxidoreductions of b and c cytochromes in ox heart mitochondria and 'inside-out' submitochondrial particles. 2. kinetic analysis shows that, in mitochondria at neutral ph, there is a restraint on the aerobic oxidation of cytochrome b566 with respect to cytochrome b562. valinomycin plus k+ accelerates cytochrome b566 oxidation and retards net oxidation of cytochrome b562. at alkaline ph the rate of ... | 1981 | 7305997 |
| occurrence of proteins immunoreactive with anti-coupling factor b in phosphorylating membrane preparations. | coupling factor b has been isolated from beef heart mitochondria, apparently in multiple forms which differ in molecular weight and specific activity. since it has no known intrinsic catalytic activity, detection and quantitation have been based upon the factor b-dependent stimulation of atp-linked activities in factor b-deficient sub-mitochondrial particles. this communication reports the development of a reliable and more universally applicable enzyme-linked immunosorbent assay (elisa) for det ... | 1981 | 7284357 |
| kinetics of cytochrome b oxidation in antimycin-treated submitochondrial particles. | it has been shown that in bovine heart submitochondrial particles, antimycin and 2-heptyl-4-hydroxyquinoline n-oxide (hqno) inhibit the oxidation of nadh, succinate, and reduced ubiquinone incompletely, the uninhibited rate being about 20-40 nmol of substrate oxidized min-1 (mg of protein)-1. by contrast, rotenone, cyanide, bal (2,3-dimercaptopropanol), and 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole [trumpower, b. l., & haggerty, j. g. (1980) j. bioenerg. biomembr. 12, 151-164] caused essentia ... | 1982 | 7150580 |
| the immunosuppressive effects of trichothecenes and cyclochlorotine on the antibody responses in guinea pigs. | immunosuppressive effects of trichothecenes of fusarium solani and fusarium nivale, t-2 toxin and fusarenon-x, and also of a mycotoxin of penicillium islandicum, cyclochlorotine, were studied by measuring the anti-2,4-dinitrophenyl (dnp) antibody responses in guinea pigs immunized with dnp-bovine serum albumin. among these mycotoxins, t-2 toxin alone suppressed strongly the anti-dnp antibody responses at a certain sublethal dose. with other mycotoxins, no effect was observed at any sublethal dos ... | 1982 | 7120038 |
| infectious agents associated with neonatal calf disease in southwestern idaho and eastern oregon. | over a 2-year period, 296 episodes of disease involving 226 1-to-60-day-old calves were referred to the caldwell veterinary teaching center. infectious disease agents were found in 96% of the cases. salmonella spp were the most frequently isolated agents from dairy calves, whereas coronavirus was the agent most commonly associated with diarrhea in beef calves. multiple agents were found in 20% of the dairy calves and in 12% of the beef calves. salmonella dublin, which is associated with severe d ... | 1982 | 7085442 |
| bovine mammary involution following intramammary infusion on colchicine and endotoxin at drying off. | effects of intramammary infusion of colchicine, endotoxin, and colchicine in combination with endotoxin on mammary involution were studied on 25 dairy cows in late lactation. both quarters of right udder halves were infused at or near drying off while left udder halves received no infusion. samples of mammary secretion were obtained 1 day prior to drying off, at drying off, and .5, 1, 2, 3, and 7 days post drying off. mammary secretion obtained during the early dry period from udder halves infus ... | 1982 | 7050191 |
| interactions of lipopolysaccharides, glycolipid, lipid a with acid soluble basement membrane collagen during thermal aggregation. | lipopolysaccharides derived from a number of escherichia coli and salmonella species were incorporated into thermal aggregates acid soluble basement membrane collagens. although most of these endotoxins were noted to inhibit basement membrane collagen aggregation, some lipopolysaccharides possessed little or no inhibitory properties. however, the lipid a derived from all of the latter lipopolysaccharides and the glycolipid from salmonella minnesota r595 were noted to be significant inhibitors of ... | 1981 | 7049550 |
| alkaline opening of imidazole ring of 7-methylguanosine. 1. analysis of the resulting pyrimidine derivatives. | column chromatography and spectroscopy have been employed in analyzing pyrimidine derivatives obtained from alkaline-treated 7-methylguanosine (7-meguo). high performance liquid chromatography (hplc) revealed that the alkaline generated products consist predominantly of two forms of ring opened 7-methylguanine (rom7gua) in equal amounts. material from both dowex 50 and sephadex lh-20 columns was readily resolvable into two hplc peaks. the species in one peak appears to be composed of formylated ... | 1982 | 7049418 |
| effects of dietary milk fat (whole milk) and propionic acid on intestinal coliforms and lactobacilli in calves. | calves fed whole milk had 2,000-fold fewer (p less than 0.001) coliforms in the cranial part of the small intestine than did calves fed skim milk (fat removed). calves fed milk with 32 mm added propionic acid had nearly 1,000-fold lower (p less than 0.001) counts of lactobacilli in the entire gastrointestinal tract than did calves fed milk without added propionic acid. | 1982 | 7049020 |
| electrical conductivity of milk for detection of mastitis. | the potential value of electrical conductivity of milk as a screening test for subclinical mastitis was evaluated. conductivity of foremilk and of postmilking strippings from 368 quarters of 92 cows was measured. infection status of quarters was determined by bacteriological analysis of strict foremilk samples. infections were classified as by primary or secondary pathogens, depending on the importance of the isolated organism as a mastitis pathogen. somatic cells were counted on foremilk sample ... | 1982 | 7047600 |
| scanning and transmission electron microscopic study of the small intestine of colostrum-fed calves infected with selected strains of escherichia coli. | colostrum-fed calves were fed milk replacer containing 10(11) enteropathogenic escherichia coli (eec) or nonenteropathogenic e coli (neec). the neec failed to colonize the small intestine or to be associated with the intestinal wall. the eec colonized the middle and caudal portions of the small intestine, and in these areas, 80% of the organisms were associated with the intestinal wall. light, immunofluorescence, scanning, and transmission electron microscopic studies demonstrated a layer of eec ... | 1982 | 7046530 |