Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influence of sexual preference and chronic hepatitis b virus infection on t lymphocyte subsets, natural killer activity, and suppressor cell activity. | to determine whether abnormalities of cell-mediated immunity are associated with chronic hepatitis b virus infection or with homosexuality, we studied 15 homo- and 11 heterosexual men with chronic hepatitis b virus infection, as well as 11 homo- and 16 heterosexual apparently healthy men. t lymphocyte subsets, natural killer activity, and suppressor cell activity were determined. homosexual men had significantly lower peripheral blood t4/t8 ratios resulting from a significant increase in t8-posi ... | 1986 | 2951429 |
| localization of motoneurons innervating individual abdominal muscles of the cat. | the motor pools of the individual abdominal muscles of the cat were localized in studies by using either intramuscular injections of horseradish peroxidase (hrp) to retrogradely label abdominal motoneurons or electrical microstimulation of the ventral horn at different segmental levels to produce localized twitches of the abdominal muscles. the segmental distribution of each motor pool was as follows: rectus abdominis, t4-l3; external oblique, t6-l3; transverse abdominis, t9-l3; and internal obl ... | 1987 | 2951401 |
| subset specificity of antilymhocyte antibodies in systemic lupus erythematosus. ii. preferential reactivity with t4 + cells is associated with relative depletion of autologous t4 + cells. | using indirect immunofluorescence and flow cytometry, we determined the proportion and number of t3+, t4+, and t8+ cells in the peripheral blood of patients with systemic lupus erythematosus whose sera were positive for cold-reactive antilymphocyte antibodies versus values in patients whose sera were negative for these antibodies. there was a disproportionate reduction in t4+ peripheral lymphocytes when cold-reactive antilymphocyte antibodies preferentially cytotoxic for this subpopulation were ... | 1987 | 2950862 |
| immunoregulatory function of t8 and t4 cells from synovial fluid of patients with chronic arthritis. | 1986 | 2950734 | |
| [current status of the treatment of laryngeal cancer]. | the state of the art in laryngeal cancer treatment in the rsfsr is outlined on the basis of an analysis of morbidity and other statistical data. measures which can be taken to considerably improve the effectiveness of treatment are discussed. the importance of a complex of diagnostic procedures to be employed in therapy planning is shown. preoperative hbo-mediated gamma therapy and mean fractionation of radiation were used to improve combined treatment. the following function-saving procedures w ... | 1987 | 2950661 |
| [hormonal, beta-endorphin and renin activity changes in man during partial immersion, as a therapeutic method, in water at 38 degrees c]. | this research was carried out to define the effects on men of head-out water immersion in a bath at 38.41 +/- 0.04 degrees c (mean +/- s.e.) with a method similar to that used for therapeutical rehabilitation and time of immersion of 30 minutes. beta-endorphin, renin activity, aldosterone, cortisol, hgh, fsh, lh, tsh, t3, t4 and prolactin haematic levels were analysed. seventeen healthy subjects (fourteen males and three females), aged 21-65 years (mean age 29.8 +/- 2.6) were studied. water imme ... | 1987 | 2950339 |
| stimulation of protein-directed strand exchange by a dna helicase. | the protein-mediated exchange of strands between a dna double helix and a homologous dna single strand involves both synapsis and branch migration, which are two important aspects of any general recombination reaction. purified dna-dependent atpases from escherichia coli (reca protein), ustilago (rec 1 protein) and phage t4 (uvsx protein) have been shown to drive both synapsis and branch migration in vitro. the t4 gene 32 protein is a helix-destabilizing protein that greatly stimulates uvsx-prot ... | 1987 | 2950327 |
| visualization of cytokine and virus receptors common to the immune and central nervous system. | interleukin 1 (il-1) and the human retrovirus htlv-iii/lav represent two distinct molecular and biological constituents that are apparently shared by both the immune and central nervous systems. il-1 is a cytokine produced principally by monocytes of the immune system and glial cells of brain in situ or in vitro. htlv-iii/lav represents a unique class of human retrovirus which is the recognized etiological vector of acquired immunodeficiency syndrome (aids) which exhibits cellular tropism to hel ... | 1987 | 2950283 |
| controlling mechanisms for expression of the bacteriophage t4 beta-glucosyltransferase gene. | the plasmid pthf3047 carries a 2200 bp fragment containing the phage t4 beta-glucosyltransferase (beta gt) gene and part of the upstream gene 42 cloned in pbr313 under the control of the amp promoter p1. in t4-infected cells the beta gt gene may be expressed by a mechanism antagonizing rho action. the plasmid pthf3047 expressed about threefold higher beta-glucosyltransferase activity in a strain carrying the polarity-suppressing rho-102 mutation than in an otherwise isogenic rho+ strain, and pro ... | 1987 | 2950203 |
| monoclonal antibodies reactive with swine lymphocytes. ii. detection of an antigen on resting t cells down-regulated after activation. | the expression of an antigen on porcine t lymphocytes detected by murine monoclonal antibody (mab) 8/1 was investigated by functional studies and dual-parameter immunofluorescence. mab 8/1 reacts with greater than 95% of thymocytes and in peripheral blood with all t lymphocytes and with cells of the monocyte/macrophage lineage, but not with b cells, erythrocytes, and platelets. pretreatment of peripheral blood lymphocytes with mab 8/1 plus complement abrogated the proliferative response in vitro ... | 1987 | 2950172 |
| a defect of immunoregulatory t cell subsets in systemic lupus erythematosus patients demonstrated with anti-2h4 antibody. | the cell surface phenotype of peripheral blood lymphocytes (pbl) of systemic lupus erythematosus (sle) patients was characterized with the anti-2h4 monoclonal antibody that defines the human suppressor inducer subset. the t4+2h4+ population of cells has been shown to be critical for the activation of t8+ suppressor cells. patients with sle has a markedly decreased percentage of t4+2h4+ cells (13 +/- 2%) in their pbl compared with normal controls (21 +/- 1%) (p less than 0.001). this reduction wa ... | 1987 | 2950134 |
| [lymphocyte subpopulations and hla-dr determinations in diseases of the inner ear and bell's palsy]. | thymus lymphocytes-subpopulations (t-helper t4 and t-suppressorcells t8) were determined in inner ear diseases (e.g. sudden hearing loss, neuronitis vestibularis, morbus menière, bell's palsy) by specific monoclonal antibodies. t-cell subset ratio (t4/t8) was elevated (less than 3) in about 50% of all patients. dr-typifying was performed because of the well-known control of the immunoregulation through the class ii hla-dr antigens. there was a relative risk of 5.2 in peripheral vestibular lesion ... | 1986 | 2950069 |
| induction of suppression following autologous mixed lymphocyte reaction; role of a novel 2h4 antigen. | in this study, we have investigated the cellular and molecular basis for immunoregulatory function of t4+ cells after autologous mixed lymphocyte reaction (amlr) activation. we demonstrated that the t4+ 2h4+ subset but not the t4+2h4- subset can proliferate maximally in response to autologous non-t cells. t4+ cells activated by amlr exerted suppressor activity on pokeweed mitogen-driven igg synthesis of autologous peripheral blood lymphocytes. the suppressor activity by amlr-activated t4+ cells ... | 1987 | 2949986 |
| suppressor cell activity in melanoma-draining lymph nodes. | we previously demonstrated that the cells of lymph nodes near to a melanoma respond well to stimulation by mitogens, alloantigens, and interleukin 2 than do nodes further away. in this study we examined suppressor t-cell activity in nodes at different distances from primary melanoma, using a concanavalin a (con a) suppressor cell assay. tumor-free regional nodes were classified as proximal, intermediate, and distal relative to primary melanoma. lymph node lymphocytes (lnl) were stimulated with 5 ... | 1987 | 2949828 |
| treatment of htlv-iii/lav-infected patients with d-penicillamine. | 13 asymptomatic, htlv-iii/lav-infected male homosexuals with generalized lymphadenopathy were treated with oral d-penicillamine. all patients had depressed t4/t8 ratios and 12 had impaired t-cell function. an escalating dose schedule was employed over 2-6 weeks with doses from 0.5 to 2 g/day. generalized skin rashes developed in 4 patients which required discontinuation of therapy in one patient. two patients developed mild transient elevations of hepatocellular enzymes. reversible decreases in ... | 1986 | 2949756 |
| alterations in helper-specific circulating t lymphocytes and in the autologous mixed lymphocyte reaction in chronic hepatitis b. | defects in t lymphocyte subpopulations and in the functional characteristics of such cells have been thought to play a role in the evolution of chronic hepatitis b, but the precise nature of the alterations and their significance remains unresolved. we studied lymphocyte subsets in 27 patients with chronic hepatitis b utilizing standard monoclonal antibodies including leu 1,2a,3a,7 and d1/12 (against the common determinant of the dr molecule), as well as a newly described monoclonal antibody, "5 ... | 1987 | 2949600 |
| immunoregulatory defect in patients with autoimmune thrombocytopenic purpura (atp). | con a-induced suppressor cells were studied in 20 patients with autoimmune thrombocytopenic purpura and 20 normal controls. percentage of suppression was determined on autologous and allogenic lymphocytes during blast transformation with phytohemagglutinin (pha). in 10 patients and 10 controls helper (t4) and suppressor (t8) subsets were also determined, using monoclonal antibodies. we found a significant decrease in patients' suppressor cell activity both with autologous and allogeneic lymphocy ... | 1986 | 2949403 |
| [effect of pind-avi on various ratios of helper and suppressor t lymphocytes]. | the immunomodulating effect of a new heterologous antigen (pind-avi) on helper (inducer) and suppressor t cells was investigated. primarily decreased t4 values (less than or equal to mean t4 - 2 s.d.) increased after treatment with pind (p less than 0.005, using student's t-test; n = 15). primarily increased t 8 values (greater than or equal to mean t8 + 2 s.d.; obtained for normal persons) decreased after therapy (p less than 0.001; n = 10). an increase of t4/t8 ratio (p less than 0.005 and les ... | 1986 | 2949105 |
| the value of lymph node biopsy in patients with the acquired immunodeficiency syndrome (aids) and the aids-related complex (arc): a morphological and immunohistochemical study of 90 cases. | the morphological and immunohistochemical findings in lymph nodes of nine patients with the acquired immunodeficiency syndrome (aids) and 81 patients with the aids-related complex (arc) are presented. three basic histological patterns were observed: follicular hyperplasia (29 cases), mixed hyperplasia (49 cases) and lymphocyte depletion (12 cases). while the first two variants were detected in typical arc patients, lymphocyte depletion was always associated with aids. immunohistochemistry on fro ... | 1986 | 2948898 |
| t8 cell suppression of antigen- and mitogen-induced t4 cell dependent immunoglobulin production. | the suppressor effect of t8 cells on antigen-induced, as compared to pokeweed mitogen-induced, t4 cell dependent immunoglobulin (ig) production by b cells of healthy subjects was studied. the antigens used were purified protein derivative of tuberculin (ppd) and tetanus toxoid (tt). the suppressor effect of t8 cells on igg, igm and iga responses in co-cultures of t4 cells and b cells was significantly stronger in the pokeweed mitogen driven system than in ppd- and tt-driven cultures under the sa ... | 1986 | 2948744 |
| human helper-t-cell function does not require t4 antigen expression. | the relationship between immunoregulatory t-cell function and the expression of t-cell subset-specific differentiation antigens was examined using a phenotypically anomalous human t-cell line (tcl), termed h-1. h-1 cells were found to express t11, extremely high levels of t3, but no t4 nor t8 antigen. despite their lack of t4 antigen expression, h-1 cells could be activated by coculture with pokeweed mitogen (pwm), anti-t3 antibody, or autologous b cells to provide potent help for b-cell differe ... | 1986 | 2948666 |
| c-reactive protein reduces the promotion of human b-cell colony formation by autoreactive t4 cells and t-cell proliferation during the autologous mixed-lymphocyte reaction. | utilizing a dual liquid/semisolid culture system with human b cells suspended in a semisolid matrix, t4 helper cells activated in the autologous mixed-lymphocyte reaction (amlr) were much more effective than t8 suppressor cells in promoting b-cell colony formation in the presence of staph protein a. c-reactive protein (crp) inhibited the promotion of b-cell colony formation by autoactivated t cells and inhibition was observed only when crp was present during the early amlr. coculture experiments ... | 1986 | 2948663 |
| physical and functional association of the t cell receptor and the t3 molecular complex on cytotoxic t cell clones that are differentially inhibitable by anti-t3 antibodies. | to examine the hypothesis that the antigen-specific t cell receptor (tcr) can function independently from the t3 complex on cytolytic t lymphocyte (ctl) clones, the physical and functional association of the t3 molecular complex and the t cell receptor has been examined on ctl clones that are differentially susceptible to inhibition by anti-t3 antibodies. from a panel of nine dpw2-specific ctl clones derived from the same donor, two clones (8.4 and 8.8) that were the most disparate in their susc ... | 1986 | 2948659 |
| characterization of a distinct subgroup of high-risk persons with kaposi's sarcoma and good prognosis who present with normal t4 cell number and t4:t8 ratio and negative htlv-iii/lav serologic test results. | three homosexual male patients with biopsy-proved kaposi's sarcoma were classified as having the acquired immune deficiency syndrome (aids) by centers for disease control criteria when first seen in 1983 and 1984. these patients, however, differed from most patients with aids and kaposi's sarcoma in having normal cd4 cell numbers and normal cd4:cd8 ratio. furthermore, these immunologic parameters remained normal for eight to 24 months of follow-up, and the disease did not progress. results of re ... | 1986 | 2948385 |
| immunoregulatory function of t8 and t4 cells from synovial fluid and blood of patients with rheumatoid arthritis or other forms of chronic arthritis. | the suppressor effect of synovial fluid (sf) t8 cells and blood t8 cells on the pokeweed mitogen (pwm)-induced t4 cell-dependent immunoglobulin production of autologous blood b cells was studied in nine patients with chronic rheumatic diseases (six patients with rheumatoid arthritis (ra), one patient with juvenile ra, and two patients with other forms of chronic arthritis). the suppressor effect of sf t8 cells was of the same magnitude as that of equal numbers of blood t8 cells from patients and ... | 1986 | 2948275 |
| localization of the t4 antigen/aids virus receptor in monkey and rat brain: prominence in cortical regions. | 1986 | 2948211 | |
| lymphocyte subsets in the young and aging marmoset (callithrix jacchus). | t lymphocytes were studied in healthy young and old marmosets (callithrix jacchus). data indicate that monoclonal antisera to human t lymphocyte antigens can be used to identify these cells in marmosets. total t and suppressor t cells were lower in the older group; there were no differences in helper t or in b cells. helper/suppressor ratios (t4/t8) were increased in the older group. thus, immune system alternations with aging and/or immune deficiency syndromes may be studied in this animal mode ... | 1986 | 2948018 |
| atrial natriuretic peptide in hypothyroidism. | the effect of hypothyroidism on circulating levels of atrial natriuretic peptide (anp) was studied in 11 hypothyroid patients (aged 11-65 yr; mean, 31 yr) and 13 normal subjects (aged 28-39 yr; mean, 32 yr). plasma anp was 32 +/- 9 (+/- sd) pg/ml in normal subjects and 20 +/- 5 pg/ml in the hypothyroid patients (p less than 0.005). in 7 hypothyroid patients, plasma anp levels were measured after 10-14 weeks of l-t4 therapy. anp increased from 22 +/- 5 to 46 +/- 18 pg/ml (p less than 0.02), along ... | 1987 | 2947909 |
| identification of a new lymphocyte subset surface antigen, the expression of which disappears after in vitro and in vivo stimulation. distribution, biochemistry, and functional studies. | two monoclonal antibodies (moabs) are described (md 2.6, igg1 and md 4.3, igg2a) that react with a nonlineage specific lymphocyte subset surface antigen. this antigen is expressed on b cells, a subset of both t8+ and t4+ cells, cells that exert killer and natural killer cell activity in vitro, b cells in lymph nodes, and a small percentage of thymocytes. expression of the antigen was found to be variable on t cells but not on b cells among individuals. following polyclonal activation, expression ... | 1986 | 2947882 |
| examination of the low proliferative capacity of human jejunal intraepithelial lymphocytes. | the proliferation of human jejunal intraepithelial lymphocytes (iel) was examined to determine how it differed from that of peripheral blood (pb) t lymphocytes. the iel were mainly t lymphocytes of the cytotoxic-suppressor (t8+) phenotype. they demonstrated lower proliferative responses to various stimuli (2,501 +/- 565 ct/min with phytohaemagglutinin; pha) compared to unseparated pb t lymphocytes (73,678 +/- 2,495) or the t8+ subset (68,939 +/- 10,053 ct/min) (p less than 0.001). this low proli ... | 1986 | 2947761 |
| suppressor cell activities in autologous human tumor systems. suppression of tumor immunity. | autologous t-cell clones or lines, established from peripheral blood lymphocytes (pbls) and lymphocytes from tumor-affected lymph nodes, were used to examine the immunoregulatory circuitry that might influence cell-mediated cytotoxic responses against human cancers. in a chromium 51-release microcytotoxicity assay, pbls, when activated in vitro against autologous tumor cells in interleukin-2, generated marked cytotoxicity against the autologous targets. in four solid-tumor systems, such generati ... | 1986 | 2947550 |
| microtubule reorganization is related to rate of heart myocyte hypertrophy in rat. | since stimulation of heart hypertrophy by pressure overload was previously shown to be accompanied by a densification of microtubule network in rat heart myocytes, we verified that similar process occurred during postnatal growth in euthyroid rats and in hypothyroid rats whose growth was stimulated by 4 micrograms/day l-thyroxine (t4). for this purpose, tubulin, the constituent protein of microtubules, was immunolabeled in myocytes isolated at various times after birth. myocyte hypertrophy was e ... | 1986 | 2947475 |
| rejection of skin grafts and generation of cytotoxic t cells by mice depleted of l3t4+ cells. | in mice, "helper/inducer" t cells can be depleted by treatment with a rat monoclonal antibody to the cell surface antigen, l3t4, which is homologous to the human antigen t4 (cd4). in order to examine the contribution of "helper/inducer" t cells to cellular immunity, c57bl/6 (h-2b) mice were treated weekly with 1 mg i.v. of a monoclonal antibody to l3t4. three days after the first injection, the mice received skin grafts from balb/c (h-2d) mice. the mice were then examined for skin graft rejectio ... | 1986 | 2947360 |
| t lymphocyte subpopulations defined by monoclonal antibodies in essential mixed cryoglobulinemia and in secondary cryoglobulinemias. | t lymphocyte subpopulations defined by monoclonal antibodies were determined in patients with essential mixed cryoglobulinemia (emc) and secondary cryoglobulinemias (sc). a decrease of circulating lymphocytes and a reduction in the absolute number of t3+, t4+ and t8+ (p less than 0.01) as well as in the percentage of t4+ lymphocytes (p less than 0.05) were found in emc. a significant decrease of t8+ cells, both in percentage (p less than 0.01) and absolute number (p less than 0.001), was evidenc ... | 1986 | 2947310 |
| theory of an immune system retrovirus. | human immunodeficiency virus (hiv; formerly known as human t-cell lymphotropic virus type iii/lymphadenopathy-associated virus, htlv-iii/lav), the retrovirus that infects t4-positive (helper) t cells of the immune system, has been implicated as the agent responsible for the acquired immune deficiency syndrome. in this paper, i contrast the growth of a "normal" virus with what i call an immune system retrovirus: a retrovirus that attacks the t4-positive t cells of the immune system. i show that r ... | 1986 | 2947242 |
| cloning of t4 gene 32 and expression of the wild-type protein under lambda promoter pl regulation in escherichia coli. | bacteriophage t4 gene 32 encodes a single-stranded dna binding protein required for t4 dna replication, recombination, and repair. previous attempts at cloning gene 32 have failed due to a presumably deleterious effect on host cell viability. in addition, overexpression of gene 32 would be expected to be limited by the autoregulatory ability of the gene 32 product g32p. a repetitive a + t-rich sequence flanking the ribosome binding site of gene 32 has been implicated in this translational regula ... | 1986 | 2947239 |
| selective loss of the suppressor-inducer t-cell subset in progressive multiple sclerosis. analysis with anti-2h4 monoclonal antibody. | the t4+ lymphocyte population includes a subset that induces suppressor t lymphocytes (t8+ cells) and can be distinguished by dual-color fluorescence analysis with anti-2h4 and anti-t4 monoclonal antibodies. to investigate the possible role of these cells in multiple sclerosis, we used anti-2h4 antibody to characterize peripheral-blood lymphocyte subsets in 63 patients with multiple sclerosis that was progressive, stable, or acute (relapsing-remitting). twenty-three of 37 patients with progressi ... | 1987 | 2946956 |
| the interleukin 2 secretion defect in vitro in systemic lupus erythematosus is reversible in rested cultured t cells. | interleukin 2 (il 2) secretion in response to mitogenic stimulation in vitro is strongly reduced in circulating t lymphocytes from patients with sle. it is still not clear how this abnormality relates to the b cell hyperactivity in the disease. some investigators proposed that an intrinsic t helper cell defect could lead to suppressor cell dysfunction and autoimmunity. others have found that in fact increased suppressor cell activity can cause il 2 hyposecretion. in the present study we report t ... | 1986 | 2946762 |
| defective suppressor cell function mediated by t8+ cell lines from patients with progressive multiple sclerosis. | activated suppressor cell function, induced with either concanavalin a or okt3 and mediated by either unfractionated mononuclear cells or "panning" enriched t8+ cells, freshly isolated from peripheral blood, is reduced in patients with progressive multiple sclerosis (ms) as compared with control donors. in this study, we generated t8+ cell lines from the peripheral blood of these same patients and controls. suppressor activity, mediated by t8+ cells exposed to okt3 on days 1, 7, and 14 of cultur ... | 1986 | 2946761 |
| the effect of nutritional support on t-lymphocyte subpopulations in protein calorie malnutrition. | t-lymphocyte subpopulations were measured in the two major types of adult malnutrition, adult marasmus and kwashiorkor-like hypoalbuminemic malnutrition. the population of t-cells (t3) and the percentage of both helper (t4) and suppressor (t8) t-cells were significantly (p less than .05) decreased in patients with kwashiorkor-like hypoalbuminemic malnutrition, but did not differ from control values in patients with adult marasmus. the ratio of helper t-cell (t4), to suppressor t-cells (t8) (rang ... | 1986 | 2946750 |
| rapid phosphorylation and modulation of the t4 antigen on cloned helper t cells induced by phorbol myristate acetate or antigen. | a cell surface protein known as t4 (cd4, leu3), mr = 55,000, is expressed on the subset of human t lymphocytes which provides helper function for b cell and cytotoxic t cell activities. we show that t4 is constitutively phosphorylated and that phorbol myristate acetate (pma) induces a rapid serine phosphorylation which is followed by a fast dephosphorylation. within 5 min after pma treatment, there is a 24% reduction of t4 on the cell surface, by 4 h the loss is nearly complete, and by 20 h t4 i ... | 1986 | 2946683 |
| structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model. | physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ... | 1986 | 2946570 |
| hypothesis: aids is an autoimmune disease directed at the immune system and triggered by a lymphotropic retrovirus. | by attaching to the cd4 (t4) molecule of the helper-inducer lymphocyte, the human immunodeficiency virus (hiv) envelope imitates the normal ligand for this receptor, namely, an invariant component of the class ii major histocompatibility antigen (mhc). depending on the degree of antigen mimicry, the normal immune response to retrovirus envelope would be expected to recognize and cross-react to self-mhc. by disguising as "self" the virus then provokes an autoimmune attack of class-ii-bearing cell ... | 1986 | 2946499 |
| [polymorphism of human thyroxine binding serum globulin (tbg); existence of a new form of serum protein without detectable triiodothyronine binding power]. | two kinds of tbg polymorphism are described in human, one found in deglycosylated tbg from individual blood donors, the other is a genetically determined polymorphism. tbg from plasma samples from a patient with toxic goiter, not autoimmune, (p)tbg, from the patient's mother (m)tbg and from individual donors (n)tbg, were labeled with [125i]t4 or [125i]t3 and submitted to isoelectric focusing (ief), followed by autoradiography. three faint [125i]t4 radiolabeled bands were detectable in (p)tbg whi ... | 1986 | 2946372 |
| cloning of the lambda resistant genes from brevibacterium albidum and proteus vulgaris into escherichia coli. | genes from proteus vulgaris atcc13315 and brevibacterium albidum atcc15831 were introduced into escherichia coli, which rendered the host resistant to coliphage lambda. the clones transformed by any one of the two recombinant plasmids, prmg101 or prmg216, were totally resistant against the infection of virulent lambda and n4, but sensitive to ø80, t4 and t7. however, when maltose transport systems of the clones were induced by maltose, the clones were no more resistant to the phage: thus, this p ... | 1986 | 2946296 |
| time relationship of immune changes to htlv-iii/lav seroconversion in patients with hemophilia. | longitudinal immune studies of patients with hemophilia a were begun in 1982 by the regional hemophilia center in st. louis, missouri. serum samples collected from 74 participants between 1982 and 1985 were analyzed for antibody to human t-lymphotropic virus type iii (htlv-iii)/lymphadenopathy-associated virus (lav). the incidence of antibody to htlv-iii/lav has increased significantly in this population of patients with hemophilia. only one of eight hemophiliacs had detectable antibody before j ... | 1986 | 2946253 |
| immunofluorescent assessment of tumour infiltrating cells in laryngeal carcinoma. application of monoclonal antibodies. | in order to gain some insight into host cell accumulations within primary tumour, frozen sections from surgical specimens of laryngeal carcinoma were subjected to indirect immunofluorescence using a panel of monoclonal antibodies against various human lymphocyte subsets as well as macrophages. in addition, polyclonal antibodies against ig were used in order to trace b cells. numerous host cell infiltrates seen at the tumour periphery were composed of t4 (helper) lymphocytes and macrophages. lymp ... | 1986 | 2946139 |
| ion etching of bacteriophage lambda: evidence that the right end of the dna is located at the outside of the phage dna mass. | bacteriophage lambda was etched in an ar+ plasma under conditions in which the capsid and some of the dna were eroded (by sputtering) from the particle surface. analysis of the dna remaining in etched phage demonstrated an enrichment in sequences derived from the left end and middle of the genome; sequences from the right end were selectively lost. the results suggest that the dna in the mature phage is arranged with its left end toward the center and its right end toward the exterior of the ove ... | 1986 | 2945932 |
| increased t-helper lymphocytes following active specific intralymphatic immunotherapy of cancer. | sixteen malignant cancer patients (seven melanoma, three colon, three lung, and three other) who received tumor vaccine therapy--14 autologous and two allogeneic--were studied for t-cell phenotyping changes. blood samples were obtained prior to therapy, and also at various time intervals following the therapy. a consistent and reproducible elevation of t3 and t4 subsets, as tested on day 21 after the therapy, was observed in 12 patients. the mean percentages of t3 and t4 fractions before and aft ... | 1986 | 2945905 |
| the role of the 2h4 molecule in the generation of suppressor function in con a-activated t cells. | the molecular basis for the suppression generated in a concanavalin a (con a)-activated t cell culture remains unknown. in this study, we have attempted to determine whether the 2h4 and 4b4 molecules on con a-activated t cells play some role in the generation of suppression by such cells. we have shown that con a-activated suppressor cells belong to the 2h4+ subset of t cells but not the 4b4+ (2h4-) subset. con a-activated t cells exerted their optimal suppressor function on day 2 in culture, a ... | 1986 | 2945865 |
| evidence for htlv-iii/lav expression by primary cultures of t8 cells. | the selective targets for htlv-iii/lav, the causal infectious agent of aids and aids-related complex (arc), are t4 cells, apparently because the virus receptor is associated with t4 antigen determinants. this accounts for t4 cell depletion in aids and for a decrease of il-2 production by aids peripheral blood lymphocytes (pbl) after in vitro pha activation. by contrast, t8 cells are not targets for htlv-iii/lav, since t8 cells from pbl and from long-term cultured t cells (ctc) could not be infec ... | 1986 | 2945790 |
| human t helper cells specific for antigens of typhus group rickettsiae enhance natural killer cell activity in vitro. | the peripheral blood mononuclear cells (pbmc) from 5 individuals immune to typhus group rickettsiae and from 13 nonimmune individuals were stimulated in vitro for 7 days with typhus group rickettsial antigen (tgra). at the end of day 7, lysis of the natural killer (nk)-susceptible target k562 by these pbmc was determined. as controls, pbmc from both groups of donors were cultured in vitro for 7 days without antigen or were freshly isolated, and lysis of the k562 target was determined. there was ... | 1986 | 2945787 |
| [determination of t4 and t8 lymphocytes in malignant melanoma of the choroid and their significance in diagnosis]. | 1986 | 2945770 | |
| a remarkable amino acid sequence homology between a phage t4 tail fibre protein and orf314 of phage lambda located in the tail operon. | we have found that the amino acid (aa) sequence of the tip of phage t4 tail fibre (gene 37) shows more than 50% homology with the aa sequence predicted from an open reading frame (orf314) in the phage lambda genome. orf314 is near the 3' end of the late morphogenetic operon, beyond gene j coding for the lambda tail fibre. the homologous sequences are for the most part composed of repeated aa, the most remarkable of which is a gly-x-his-y-his motif where x and y are small, uncharged aa, found six ... | 1986 | 2945762 |
| [lymphocytic interstitial pneumopathy in aids-related complex. presence of the lav virus in the bronchoalveolar lavage fluid]. | a new case of lymphocytic interstitial pneumonitis developed in the course of a persistent generalized lymphadenopathy syndrome is reported. the patient was a 30-year old haitian woman with only her ethnic risk factor. broncho-alveolar lavage showed high cellularity with mostly major lymphocytosis (76%) and a fall of the ok t4/ok t8 ratio to 0.23. the lav was isolated from the lavage fluid lymphocytes on the same day and within the same culture time as from blood, using lymphocyte culture and me ... | 1986 | 2945180 |
| immunoglobulin synthesis and secretion by leukemic b cells from patients with chronic lymphocytic leukemia. | we investigated the ability of purified e-rosette negative largely leukemic b cells from 15 patients with b-cell chronic lymphocytic leukemia (cll) to synthesize and secrete igm, iga and igg spontaneously or in the presence of purified autologous or allogeneic t4 cells from normal donors, in pwm-induced differentiation system. we observed moderate but significant igm synthesis and secretion (19.7 +/- 8.9 micrograms/dl, n = 5) by leukemic b cells alone in 5 of 15 patients examined. these igm conc ... | 1986 | 2945055 |
| suppressor t lymphocytes from lepromatous leprosy skin lesions. | the immune response in leprosy forms a spectrum with lepromatous leprosy patients exhibiting specific unresponsiveness to antigens of mycobacterium leprae. this unresponsiveness is thought to be related to the prevalence of t8-positive lymphocyte in these lepromatous lesions. to analyze the immunoregulatory function of these t8 cells, we developed simple procedures to extract lymphocytes from skin biopsy specimens of patients with leprosy. these lymphocytes were sorted for t8 and t4 positive cel ... | 1986 | 2944966 |
| possible t4-hla class ii interaction as an essential event in antigen-specific helper t lymphocyte-dependent b cell activation. | we have previously shown that the interaction between influenza virus-specific helper t lymphocytes and b lymphocytes is hla class ii restricted (fischer, a. et al., eur. j. immunol. 1985. 15: 620). in the present study, we used a panel of antibodies specific for the t4 and hla-dr molecules to investigate the role of both structures in t-b lymphocyte interaction. several anti-t4 monoclonal antibodies were shown to block the in vitro antibody production to a/bangkok influenza virus while they wer ... | 1986 | 2944748 |
| inhibition of n-linked oligosaccharide trimming mannosidases blocks human b cell development. | deoxymannojirimycin (dmm) or swainsonine (sw), which block conversion of high-mannose to complex-type n-linked glycans, strongly inhibited the production of immunoglobulin (ig) when added to cultures of human lymphocytes together with the polyclonal b cell activators pokeweed mitogen (pwm) and staphylococcus aureus (sac). to obtain the inhibitory effect, inhibitor had to be present during the first 36 h of culture. addition at later timepoints was less effective and showed that neither inhibitor ... | 1986 | 2944744 |
| histamine inhibits interferon-gamma production via suppression of interleukin 2 synthesis. | histamine, a modulator of various immune functions, inhibits the production of interleukin 2 (il-2) and interferon-gamma (ifn-gamma) by polyclonally activated human blood mononuclear cells. the histamine-induced inhibition of ifn-gamma synthesis can be completely eliminated by the addition of recombinant il-2. the ifn-gamma synthesis by t8+ lymphocytes is highly dependent on il-2 supplied either by the il-2 producing t4+ lymphocytes or through exogenous addition of recombinant il-2. it is conclu ... | 1986 | 2944612 |
| accessory function of thymic and tonsillar dendritic cells in interferon gamma production by t lymphocytes. | human dendritic cells (dc) were purified from tonsils and thymus by a three-step procedure. over 85% of cells in the purified dc preparations were identified as dc based on their characteristic morphology, presence of hla-dr, pro-iml and pro-im2 antigens, and absence of macrophage associated antigens okm1 and okm5. to study the accessory function of dc in ifn-gamma production, t lymphocytes and t4+ or t8+ subsets were purified from peripheral blood and tonsils. addition of autologous or allogene ... | 1986 | 2943948 |
| selective inhibition of escherichia coli recbc activities by plasmid-encoded gams function of phage lambda. | the gam locus of bacteriophage lambda encompasses two coding sequences with the same reading frame and translational stop, one corresponding to an mr 11646 polypeptide (gams gene), the other to an mr 16349 polypeptide (gaml gene). a dna segment encoding gams but not gaml was placed under lambda pr promoter control (regulated by the cits857-coded repressor) on a multicopy plasmid, and an insertion mutation (gams201) was constructed. expression of gams+, but not gams201, inhibited escherichia coli ... | 1986 | 2943636 |
| okt4/okt8 ratio and serum beta 2-microglobulin in mycosis fungoides and chronic benign dermatitis. | pre-treatment serum beta 2-microglobulin (s b2-m) and okt4/okt8 ratio (t4/t8 r) were studied in 15 patients with mycosis fungoides (mf) and in 10 subjects with chronic superficial benign dermatitis (csbd) in order to verify whether these parameters may lend support to an earlier differential diagnosis. s b2-m levels and t4/t8 r showed no significant difference in csbd as compared to normal controls. mf patients displayed elevated s b2-m and t4/t8 r values in comparison to healthy controls and su ... | 1986 | 2943593 |
| receptors for the third component of complement: their association with maturation stage in non-hodgkin lymphomas (nhl) and their possible implication with the development of follicular structures. | in the majority of 188 non-hodgkin lymphomas (nhl) investigated in this study, we found a simultaneous expression of the receptors for c3b and c3d (cr1 & cr2; cccorr = 0.69, p less than 0.0005). an analysis of the different histological entities of the kiel classification revealed that this coexpression was most pronounced for germinal centre-derived (cccorr = 0.63, p = 0.0004) and immunocytic nhl (cccorr = 0.86, p = 0.0024), whereas in chronic lymphocytic leukaemias there tended to be a more he ... | 1986 | 2943542 |
| phenotypic and functional evaluation of suppressor cells in normal pregnancy and in chronic aborters. | to evaluate the potential role of immunoregulatory cells modulating the maternal immunologic response during pregnancy, we carried out phenotypic and functional studies in patients with normal obstetrical histories during each trimester and in patients with chronic idiopathic spontaneous abortions. using monoclonal antibodies (ortho), total numbers of t cells (t3+) and t4+ cells progressively increased during pregnancy (compared to nonpregnant controls) and then declined in the third trimester. ... | 1986 | 2943429 |
| the cellular basis for differential lymphokine responses to mitogen stimulation. | human mononuclear cells from some individuals produce macrophage migration inhibition factor (mif) when stimulated with con a while those of others produce migration stimulation factor (mstf). t cells were responsible for these different responses but t4 cells produced mif and t8 cells produced mstf regardless of the global response which was not explained by the individual t4:t8 ratios. admixing the t-cell subpopulations in vitro revealed that mif responses switched to mstf responses between t4 ... | 1986 | 2943417 |
| expression of human t antigens in interspecies hybridomas. | interspecies hybrids were constructed by fusing normal human male peripheral blood mononuclear cells with bw5147, a hgprt- thymoma derived from an akr mouse. hybrid cells were selected in hat media in culture dishes containing 1 x 10(7) human red blood cells. twelve weeks after fusion, hybridomas were diluted to 10-15 cells/well and characterized for their expression of the human immune cell surface antigens hla-dr, t3, t4, and t8 using fluorescent microscopy and cytographic analysis. more than ... | 1986 | 2943408 |
| functional capacities of t lymphocyte subsets from synovial fluid and blood in rheumatoid arthritis. | a reverse haemolytic plaque forming cell (pfc) assay was employed to analyse the impact of t suppressor/cytotoxic and t helper cells on b cell function in 10 patients with rheumatoid arthritis (ra). in all cases t8-enriched cells from synovial fluid and blood suppressed the pokeweed mitogen (pwm) induced igm, igg, and iga secretion by autologous lymphocytes to the same degree. the suppression was partly abolished by irradiation of t8-enriched cells. t4-enriched cells from blood increased the pwm ... | 1986 | 2943237 |
| human cord blood suppressor t lymphocytes. ii. characterization of inducer of suppressor cells. | previously, we reported an antigen nonspecific inducer of t suppressor cell factor (tisf) produced by cord blood mononuclear cells (mnc) in 48-hr, two-way mixed lymphocyte cultures (mlc). the target of this factor was a radiosensitive, t4+ (t8-) adult suppressor t cell subset. the cellular origin of this tisf was examined in the present study. igg production by pokeweed mitogen (pwm)-stimulated adult mnc was used as an assay for tisf activity. it was found that tisf-producing cells formed rosett ... | 1986 | 2943171 |
| evaluation of the functional maturity of newborn t8+ suppressor cells and the resistance of newborn lymphocytes to suppression. | concanavalin a (con a) was used to study the suppressor function of human umbilical cord blood t cells. the suppressor activity and the phenotype of con a-activated adult and cord t cells were compared using allogeneic adult mononuclear cell (mnc) response to pokeweed mitogen (pwm), phytohemagglutinin (pha), and alloantigen as indicator culture systems. it was found that con a-activated adult suppressor t cells were predominantly t8+ cells and suppressed pwm-induced igg production by adult lymph ... | 1986 | 2943170 |
| [interstitial lymphoid pneumonia and polyadenopathies in patients infected with the lav/htlv iii virus]. | seven patients of haitian and central african origin were investigated for interstitial pneumonitis and unexplained chronic lymphadenopathy. a study of broncho-alveolar lavage fluid showed no opportunistic micro-organisms and disclosed a high alveolar lymphocyte count with more than 80% t8+ cells. in 3 patients, open lung biopsy showed lymphoid interstitial pneumonitis. follicular hyperplasia was a constant finding in lymph nodes. benign lymphocytic infiltration was found in other organs of all ... | 1986 | 2942907 |
| genetically restricted suppressor t-cell clones derived from lepromatous leprosy lesions. | leprosy is a spectral disease in which immune responses to mycobacterium leprae correlate with the clinical, bacteriological and histopathological manifestations of disease, so study of its pathology provides insights into immunoregulatory mechanisms in man. at the tuberculoid pole, patients have few lesions in the skin which contain rare organisms and are able to mount strong cell-mediated immune responses to m. leprae antigens. in contrast, at the lepromatous pole, patients have disseminated s ... | 1986 | 2942780 |
| comparison of diethylstilbestrol, cyproterone acetate and medroxyprogesterone acetate in the treatment of advanced prostatic cancer: final analysis of a randomized phase iii trial of the european organization for research on treatment of cancer urological group. | patients with previously untreated category t3 to t4 mo or ml prostatic cancer were allocated randomly to receive 250 mg. cyproterone acetate per day, a loading dose of 500 mg. medroxyprogesterone acetate intramuscularly 3 times weekly for 8 weeks followed by 100 mg. orally twice daily, or 1 mg. diethylstilbestrol 3 times daily in a phase iii trial (protocol 30761) performed by the genitourinary tract cooperative group of the european organization for research on the treatment of cancer. of 236 ... | 1986 | 2942707 |
| transient immunoregulatory perturbation during the acute phase of rheumatic fever. | an abnormal immune response directed at streptococcal antigen is currently thought to be involved in the development of autoimmune phenomena in rheumatic fever (rf). we studied t-cell subsets using the okt monoclonal antibodies in 13 patients with newly-diagnosed rf. no difference was found in the total number of t cells or in the proportion of helper t cells (okt4+) at the acute phase (56.6 +/- 6.9) as compared to the recovery phase (55.2 +/- 9.2). however, a significantly decreased proportion ... | 1986 | 2942691 |
| lymphocyte subsets at different stages of subacute sclerosing panencephalitis: a study with monoclonal antibodies. | lymphocyte subsets in cerebrospinal fluid (csf) and peripheral blood were studied using monoclonal antibodies, in patients with subacute sclerosing panencephalitis, eight of whom were at stage 2 and seven at stage 4. eighteen subjects affected with non immunological diseases constituted the controls. regardless of the stage, patients with subacute sclerosing panencephalitis had lower percentages of okt3+ (pan-t) cells in both csf and peripheral blood, with an increase of okia+ cells (b cells, ma ... | 1986 | 2942644 |
| lung t cells in hypersensitivity pneumonitis: phenotypic and functional analyses. | cells recovered from bronchoalveolar lavage (bal) and tissue sections from transbronchial lung biopsies were studied in 16 patients with symptomatic hypersensitivity pneumonitis (hp) and in six subjects with a similar history of exposure but without features of disease by using a series of monoclonal antibodies (moab) detecting different lymphocyte subpopulations, including t and t subsets, b lymphocytes, and natural killer (nk) cells. their functional activities in cytotoxic and suppressor assa ... | 1986 | 2942600 |
| beta-endorphin and its congeners in rat pituitary and thyroid: effects of propylthiouracil and thyroid hormone administration. | immunoreactive beta-endorphin (ir-beta ep) and immunoreactive n-acetyl-endorphin (ir-nacep) have been demonstrated in the rat thyroid by specific ria and characterized by reverse phase hplc. in addition, pituitary and thyroid ir-beta ep and ir-nacep levels have been determined after manipulation of the pituitary-thyroid axis by chronic (21 days) treatment with 1) propylthiouracil (ptu), 2) l-t4, 3) l-t3, or 4) t3 plus ptu. no difference in anterior pituitary or neurointermediate lobe ir-beta ep ... | 1986 | 2942390 |
| decrease in helper (t4+) lymphocytes following cimetidine treatment for duodenal ulcer. | we studied the possible in vivo influence of cimetidine on peripheral blood lymphocyte (pbl) subpopulations defined with monoclonal antibodies (moab) in eight haematologically normal patients with uncomplicated duodenal ulcers before cimetidine treatment, 1 week after the start, and finally 1 week following cessation of therapy. after cimetidine had been given for 3-5 weeks there was a significant decrease, compared with pretreatment numbers, in the proportion of t3+ cells (64.6 +/- 8.1 (mean +/ ... | 1986 | 2942318 |
| defective autologous mixed lymphocyte reactivity in multiple sclerosis. | t cells from patients with multiple sclerosis (ms) and normal controls were assessed for their ability to respond in the autologous mixed lymphocyte reaction (amlr). cells from stable ms patients demonstrated a significant defect in their proliferative response to non-t cells in comparison to normal controls. despite the defective amlr response, t cells from ms patients reacted as well as t cells from normal controls to allogeneic stimuli. furthermore, ms non-t-cells were fully capable of stimul ... | 1986 | 2942317 |
| chronic t cell leukemia with unusual cellular characteristics in ataxia telangiectasia. | a 27-year-old male patient with ataxia telangiectasia (at) developed atypical chronic lymphocytic leukemia with increasing bone marrow infiltration in the absence of organomegaly. one-third of the leukemia cells expressed a mature suppressor/cytotoxic t cell phenotype (t3+ t4- t6- t8+ t10-), two-thirds demonstrated additional helper/inducer t cell-associated antigens (t3+ t4+ t6- t8+ t10-), and a small fraction reacted with a natural killer (nk) cell-specific monoclonal antibody (leu 11+). the p ... | 1986 | 2942200 |
| the malignant cells in a lennert's lymphoma are t lymphocytes with a mature helper surface phenotype. a multiparameter flow cytometric analysis. | the flow cytometric analysis of dna content in cells obtained from a case of lennert's lymphoma demonstrated the presence of a discrete hypotetraploid cell population. correlated multiparameter analysis of dna, light scatter, and surface antigens by flow cytometry showed that the hypotetraploid cells were intermediate to large cells expressing t11, t3, and t4 antigens and lacking b1 and t8 antigens. these findings suggest that lennert's lymphoma represents a malignant neoplasm of t-helper lympho ... | 1986 | 2942199 |
| biochemical evidence for cellular dedifferentiation in adult rat cardiac muscle cells in culture: expression of myosin isozymes. | myosin isozyme pattern in adult rat cardiac ventricular muscle cells in long-term culture was investigated. the myosin isozymes profile of cultured cardiac myocytes underwent a change in a serum-containing medium from two weeks onward, showing an embryonic rat ventricular myosin isozymes pattern that contained predominant isozyme v3. when adult cardiac myocytes were grown in a serum-containing medium supplemented with t4, these cells contained a predominant v1 band whose electrophoretic mobility ... | 1986 | 2942141 |
| activated t cells with immunoregulatory functions at different sites of involvement in sarcoidosis. phenotypic and functional evaluations. | cells from recovered bal fluid and from infiltrates in different involved tissues (lungs, lymph nodes, conjunctiva, liver, spleen, and skin) were studied in 22 patients with active sarcoidosis in order to define the surface phenotype, functional in vitro properties, and topographic distribution of the cells in granulomatous lesions. our data demonstrated a compartmentalization of activated t cells with immunoregulatory functions from the blood to all sites of disease activity. in fact, these cel ... | 1986 | 2942079 |
| predictive value of bronchoalveolar t cell subsets for the course of pulmonary sarcoidosis. | to study the value of knowing the proportions of bronchoalveolar t cell subsets when predicting the course of pulmonary sarcoidosis, we subjected 31 patients to clinical, physiologic, and radiographic evaluations, with controls, for at least 12 months. initially, when all patients were untreated, bal's were performed, and bal lymphocyte subsets were marked by the following monoclonal antibodies: okt3 (expressed by all t cells), okt4 (to mark helper-inducer t cells), okt8 (to mark suppressor-cyto ... | 1986 | 2942077 |
| alterations in cardiac myosin isoenzymes distribution as an adaptation to chronic environmental heat stress in the rat. | the distribution of cardiac myosin isoenzymes, using gel pyrophosphate electrophoresis, was studied in laboratory rats during acclimation to heat (34 degrees c, 0-2 month) with and without daily administration of triiodotyronin (0.3 micrograms/100 g b.wt) thyroxin (t4) and triiodotyronin (t3) concentrations during that period were measured in the acclimated rats as well. control rats exhibited only the v1 myosin form during the entire experimental period. in the heat acclimated rats, after three ... | 1986 | 2941591 |
| in vivo activated peripheral t cells in autoimmune disease. | starting from the observation, if that in patients suffering from inflammatory bowel diseases elevated numbers of activated peripheral immunocytes can be detected in correlation to the activity of the disease, subpopulations of lymphocytes in immune-mediated disorders were analyzed for the expression of activation associated antigens. it was found that in patients with immunovasculitis, sarcoidosis, m. behçet, multiple sclerosis, antibody-mediated hemophilia, sle,--but not in those with sclerode ... | 1986 | 2941589 |
| proliferative signals for suppressor t cells. helper cells stimulated with pokeweed mitogen in vitro produce a suppressor cell growth factor. | to define molecular signals elaborated by inducer populations supporting growth or differentiation of t8+ cells, we collected supernatants of pokeweed mitogen (pwm)-stimulated cultures depleted of t8+ cells. when added to purified t8+ cells, these supernatants caused significant proliferation. pwm plus interleukin 2 (il-2) in amounts equivalent to those in the supernatant could not reconstitute the response caused by the supernatant. t8+ cells activated by supernatants obtained from pwm-pulsed t ... | 1986 | 2941453 |
| proliferative and cytotoxic responses of human peripheral blood lymphocytes to autologous malignant effusions. an analysis at the clonal level. | peripheral blood lymphocytes from five patients were stimulated initially in mixed lymphocyte:tumour culture (mltc) with autologous malignant effusions and cloned by limiting dilution in interleukin-2 prior to phenotyping and assay for different functional capabilities namely proliferative responsiveness to autologous and allogeneic tumours in the primed lymphocyte test (plt) and cytotoxicity (ctx) toward a range of fresh tumour and cell line targets. for individual clones the two functional act ... | 1986 | 2941144 |
| the alpha-glucosyltransferases of bacteriophages t2, t4 and t6. a comparison of their primary structures. | with the aim of comparing the primary structures of gene products coded for by t-even bacteriophages we constructed clone libraries of the dnas of bacteriophages t2 and t6. using hybrid m13 phages carrying the gene for the t4-coded alpha-glucosyl transferase (alpha gt) we isolated corresponding t2 and t6 clones. the nucleotide sequences of the three alpha gt genes and the amino acid sequences derived were compared. the differences between the genes and their products are discussed in terms of st ... | 1986 | 2940438 |
| activated suppressor cell dysfunction in progressive multiple sclerosis. | concanavalin a (con a)-induced suppressor activity has previously been shown to be reduced in multiple sclerosis (ms) patients with active clinical disease. in this study, we demonstrate that okt3, as well as con a induced suppressor activity mediated by unfractionated peripheral blood mononuclear cells is reduced in patients with the progressive form of ms. by performing reconstitution experiments involving e+, t4+, or t8+ cells derived from either ms patients or controls, and normal allogeneic ... | 1986 | 2940299 |
| functional characterization of peripheral blood lymphocytes in chronic hbsag carriers. | increased proportions of suppressor/cytotoxic t cells have been identified in the peripheral blood of chronic hbsag carriers and to investigate a possible relationship to t cell cytotoxicity against autologous hepatocytes, suppressor cell activity, viral replication and the histological type of disease, 42 consecutive hbsag carriers undergoing a liver biopsy have been investigated. the proportion of suppressor/cytotoxic lymphocytes directly correlated with t cell cytotoxicity to autologous hepat ... | 1986 | 2940037 |
| lymph node interdigitating reticulum cell sarcoma. | a 67-year-old man presented with a malignant tumor involving abdominal lymph nodes, spleen, liver, and lungs, with associated protracted fever and night sweats. the tumor consisted of large pleomorphic cells, often surrounding microabscesses. intracytoplasmic s100 protein, surface t6, leu-3a (t4), and hla-dr antigens were demonstrated. the malignant cells also possessed atpase activity. ultrastructurally, the cells exhibited numerous interdigitating cell processes but no birbeck granules. the an ... | 1986 | 2939710 |
| potential length determiner and dna injection protein is extruded from bacteriophage t4 tail tubes in vitro. | bacteriophage t4 tails contain a set of extended protein molecules in the central channel of the tail tube through which the dna must exit during infection. treatment of tails with guanidine hydrochloride separates the baseplates, leaving the tail tube and several specific tube-associated proteins. methods were developed to purify these structures. using specific antisera, immunoblotting, and electrophoretic analysis, these structures were shown to contain proteins gp19, 29, and 48. electron mic ... | 1986 | 2939620 |
| direct demonstration of immunoregulatory t-cell defects in patients with systemic lupus erythematosus. | the present study was undertaken to determine directly whether immunoregulatory t cells have a defective suppressor function in patients with systemic lupus erythematosus (sle), and whether anti-t-cell antibodies are essential for immunoregulatory t-cell defects. peripheral blood t cells and t-cell subsets were determined in 52 sle patients. the ratio of t4 to t8 cells was distributed over a wider range in patients with sle than in the controls. patients with sle were divided into three groups ( ... | 1986 | 2939553 |
| concanavalin a induction of suppressor activity in the t-helper subset defined by monoclonal antibodies. | human monocyte-depleted peripheral blood mononuclear cells were separated in t4+ and t8+ populations by a panning technique. petri dishes coated with goat anti-mouse antibodies were plated by peripheral blood mononuclear cells coated by monoclonal antibodies, either t4 or t8. the cell populations were separated into adherent and non-adherent populations based on binding to the goat anti-mouse-coated plastic dishes. the purity of the adherent populations was 96%. t4+ and t8+ populations were used ... | 1986 | 2939549 |
| immunopathology of renal allograft rejection analyzed with monoclonal antibodies to mononuclear cell markers. | the composition of the mononuclear cell infiltrate in rejecting renal allografts was determined on 96 renal biopsies and 22 nephrectomy specimens by the use of monoclonal antibodies to mononuclear cell surface markers and an indirect immunoperoxidase staining technique. during rejection the composition of the infiltrate was heterogeneous, with t cells (t11), monocytes (okm1) and hla-dr expressing mononuclear cells the most frequent sub-populations. b cells (b1) and activated t cells, identified ... | 1986 | 2939279 |
| in vitro effects of thyroid hormones on red blood cell ca++-dependent atpase activity. | thyroid hormones (th) have been shown to exert a direct stimulatory effect on the ca++-dependent atpase from human and other mammalian erythrocytes. in this in vitro system, t4 has been shown to be more effective than t3. in the present study, th effects on ca++-dependent atpase were investigated, using rabbit and human erythrocyte membranes, after preincubation with 10(-10) m t4, in the presence or in the absence of exogenous calmodulin (cam) (5.10(-12) m to 5.10(-9) m). ca++-dependent atpase a ... | 1986 | 2939127 |
| effects of thyroid hormone on cardiac size and myosin content of the heterotopically transplanted rat heart. | infrarenal heterotopic cardiac isografts maintain structural and functional integrity. we have used this transplantation model to further explore the mechanisms of thyroid hormone-induced cardiac hypertrophy. thyroid hormone administration, 1-thyroxine (t4) 10 micrograms/animal per d, led to a significant 30% increase in total heart weight and a 40% increase in the myosin content of the in situ heart when compared with control. in contrast, t4 treatment was without effect on the heart weight, pr ... | 1986 | 2939104 |
| failure of dehydroepiandrosterone enanthate to promote growth. | adrenal androgens may promote pubertal growth. to assess this possibility, we administered dehydroepiandrosterone (dhea) enanthate in monthly im injections in a dose of 70 mg/m2 for 1 yr to five boys with constitutional short stature (aged 11-13 4/12 yr) and one boy (aged 13 4/12 yr) with panhypopituitarism (coincidentally receiving t4 and human gh). all had bone age delay of at least 3 yr and subnormal levels of dhea and dhea sulfate (dhea-s) for their chronological age. pretreatment growth vel ... | 1986 | 2939100 |
| purification and characterization of the t4 bacteriophage uvsx protein. | gene uvsx of bacteriophage t4 encodes a 40,000-dalton protein that plays a key role in the major pathway for genetic recombination in t4-infected cells. mutations at the uvsx locus lead to increased sensitivity to various dna-damaging agents, reduced phage bursts, decreased genetic recombination, and early arrest of dna synthesis. like the escherichia coli reca protein, the purified uvsx protein is a dna-dependent atpase that catalyzes pairing between homologous single- and double-stranded dna m ... | 1986 | 2939071 |